Dissertations / Theses on the topic 'Seroprevalencia'
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Rojas, Guerrero Wilder Wilfredo. "Seroprevalencia de Brucella sp. en caprinos de Huarochirí-Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2004. https://hdl.handle.net/20.500.12672/2260.
Full textBrucella sp. is a Gram negative bacteria, optional intracellular, that can affect several animal species, including men, producing the disease known Nke brucelosis. Goats are considered like the disease carrier. Our country has Z047,718 of goats, 184,181 animals are in Lima. This study purpose was the seroprevalence determination of Brucella sp.in Goats from Huarochiri , Lima. There have been recollected 384 blood samples from female, goats with more than 6 months of age, without vaccination, since July up to december 2002, in the districts of San Mateo, Matucana, Surco, San Bartolomé, Huarochirí, Mariatana, Tupicocha, Santo Domingo de Olleros, Langa and Antioquia; for the Antibodies detection, using the Rosa de Bengala Technique. There was found that the 100% of animais were negative to the test. This study result proves a 0% seroprevalence.
Tesis
Velasquez, Vila Stephanie Ivonne. "Seroprevalencia de toxocariasis humana en estudiantes de medicina veterinaria." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2018. https://hdl.handle.net/20.500.12672/10002.
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Bautista, Rojas Lucy Ingrid. "Seroprevalencia de leptospirosis en ovinos en dos localidades de Puno." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2013. https://hdl.handle.net/20.500.12672/14625.
Full textDetermina la prevalencia de la Leptospira interrogans en dos localidades del departamento de Puno: Estación experimental ILLPA –INIA (Paurcarcolla)y la Ganadería Asiruni. Se muestrearon 100 sueros de ovinos de diferentes edades y sexos usando la prueba serológica de aglutinación microscópica (MAT) a fin de detectar anticuerpos frente a nueve serovares: canicola, icterohaemorrhagiae, bratislava, pomona, hardjo, australis, pyrogenes, ballum y giorgia, durante los meses de marzo y mayo del 2006. La prevalencia total hallada fue de 57%, correspondiendo a la Estación Experimental ILLPA (INIA) un 56.8% y a la Ganadería Asiruni (57.14%). Siendo reactores a los nueve serovares evaluados, observándose mayor prevalencia de ballum, icterohaemorrhagiae y australis con títulos hasta 1:400. No se determinó de una manera clara que el sexo sea un factor de riesgo por la poca cantidad de machos que había, mientras que en el factor edad, se encontraron reactores en los tres estratos etarios que se evaluaron. Se demuestra la presencia de la infección por Leptospira interrogans en ovinos en la zona ganadera evaluada.
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Zavala, Carlos Magali I. "Seroprevalencia de Brucella canis en caninos del distrito de Pucusana." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2011. https://hdl.handle.net/20.500.12672/4912.
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Meza, Cristóbal Alan. "Seroprevalencia de brucelosis bovina en el distrito de Puerto Inca, Huánuco." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2008. https://hdl.handle.net/20.500.12672/2863.
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Silva, Sánchez Pablo Saúl. "Seroprevalencia de Neospora caninum en bovinos lecheros del Valle de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2002. https://hdl.handle.net/20.500.12672/3122.
Full text--- The reproductive problems in dairy cattle produced for Neospora caninum have a great economic impact and world-wide distribution. The aim of this study was found the seroprevalence of Neospora caninum in dairy bovines from the valley of Lima in 2000. was evaluated with IFAT 304 serum samples of 19 dairy herds from north (n = 12) and south (n = 7) Lima. 29.61± 5.13% (90/304) presented antibodies against this parasite in 1:200 dilution. 40.83% ± 8.79% (49/120) in north zone and 22.28% ± 6.01% (41/184) in the south zone. Which confirm the presence of Neospora caninum in dairy bovines in the valley of Lima.
Tesis
Ventocilla, Guerra Sandra Paola. "Seroprevalencia de Brucella spp. en bovinos del distrito de Tarma-Junín." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2006. https://hdl.handle.net/20.500.12672/679.
Full text--- The objective of this study was to determine the presence of Brucella spp. in cattle of the district of Tarma, Junín, through the detection of antibodies in blood using the Rose Bengal test. A total of 344 serum samples were analysed and were not positive animals (seroreactors). This indicated the absence of Brucella spp. or a lower prevalence to 1% in the studied population. The results suggest a strict plan of monitoring epidemiologist and also the implementation of an eradication and control program for bovine brucellosis in the district of Tarma with the purpose of maintaining the area “free” of the disease. Key Words: bovine brucellosis, Brucella spp., seroprevalence, Rose Bengal, antibodies.
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Galindo, Huamán David Javier. "Seroprevalencia del virus de la rinoneumonitis equina en caballos (Equus caballus) del Perú." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2014. https://hdl.handle.net/20.500.12672/4353.
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Moya, Funcke Raúl Guillermo. "Seroprevalencia de Neospora caninum en llamas hembras de la provincia de Melgar, Puno." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2003. https://hdl.handle.net/20.500.12672/855.
Full textThe parasitic etiology reproductive problems constitute a limitation in the breeding of south american camelids. Neosporosis, caused by the prozooan Neospora canium, has been associated with reproductive problems in cattle worldwide, and it has been demonstrated e a variety of animal species, as the camels. The aim of this work was to found the seroprevalence of Neospora canium. The 16.72±4.41% (46/275) of the serum samples had antibodies at 1:50 dilution, this seroprevalence is moderate. The seroprevalence found in the her of Santa Rosa was low, with 4.65±4.45(4/85) and in the herd of Nuñoa it was ,moderate with 22.22 ±5.93%(42/189), existing significant statistical differences between both herds. This study confirms the presence of Neospora canium in female llamas from Melgar province, Puno.
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Chilón, Cornejo Vicente Jesús. "Seroprevalencia de Encephalitozoon cuniculi en conejos destinados como mascotas en la provincia de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2014. https://hdl.handle.net/20.500.12672/3807.
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García, Alamo Bruno Roberto. "Seroprevalencia y distribución geográfica de cisticercosis porcina en caseríos rurales del departamento de Tumbes." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2009. https://hdl.handle.net/20.500.12672/701.
Full textPorcine cysticercosis by Taenia solium has a wide global distribution, mainly in Asia, Africa and Latin America. In our country the disease is endemic in rural areas, which causes huge losses in small pig farms, is also a health risk public. In this study were evaluated, 97% (1872/1927) of pigs from seventeen rural villages in the province of Tumbes, in the department of Tumbes. These animals were higher at 7 months old and evaluated by EITB test to determine the level of the disease. Subsequently, the variables were assessed village of origin, age and sex to determine whether there was association with regard to the presentation of the disease, to this end made the Chi square test and logistic regression analysis, which used the statistical program STATA 9.2. The results showed that the probability of finding an infected pig in the village of Pechichal was not statistically different with respect to the village of Plateros, while the remaining villages in this probability was higher in relation to Plateros. Regarding the age of animals, found that the older animals at 12 months had a higher probability of finding parasites of pigs. But for the variable no association was found for the production of swine cysticercosis. Levels of infection found in this study for each village were in the range of 17 ± 9.2 % to 70 ± 7.8 %, with a seroprevalence of 45 ± 2.3% for the entire pig population evaluated in this study. These results show that porcine cysticercosis is a serious public health problem for the villages surveyed.
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Conislla, Limaylla Dayanne. "Seroprevalencia de los marcadores infecciosos de VHB (HBsAg y Anticore VHB) y VHC (Anti VHC) en predonantes que acudieron al Banco de Sangre del Hospital Nacional Dos de Mayo durante el periodo 2011-2014." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2015. https://hdl.handle.net/20.500.12672/4476.
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Moreno, Beas Eduardo Alejandro. "Seroprevalencia de nueve serovares de Leptospira interrogans en carnívoros, ungulados y primates silvestres en cautiverio." Tesis, Universidad de Chile, 2012. http://repositorio.uchile.cl/handle/2250/131550.
Full textSe recolectaron 130 muestras de suero desde 236 animales de los Órdenes Carnivora, Ungulata y Primates mantenidos en cautiverio en el Parque Zoológico Buin Zoo, y se determinó la presencia de anticuerpos para 9 serovares de Leptospira interrogans usando la Prueba de Microaglutinación (MAT). Trece (10%) de los sueros fueron reaccionantes a uno o más de los serovares. No existió diferencia significativa entre sexos donde 8,6% y 11,7% de las hembras y los machos respectivamente, fueron positivos. La edad tampoco resultó ser determinante, pese a que el 84,6% de los seropositivos son >36 meses. La ubicación dentro del zoológico no tiene relación significativa con los seropositivos, pese a que del total de seroreactivos, un 53,8% pertenecieron al sector C. Sin embargo, la seroprevalencia fue significativamente alta (p<0.05) en Ungulados (20,4%) en comparación a Carnívoros (3,8%) y Primates (3,4%). De los siete serovares detectados en este estudio, el más frecuente fue Autumnalis, presente en el 53,4% de los seropositivos. La mayoría de los animales reaccionantes presentaron títulos ≤1:200, a excepción de un Chrysocyon brachyurus que presentó una titulación de 1:400 frente al serovar Hardjo. En conclusión, la población ha estado expuesta a L. interrogans, pero presentando una baja seroprevalencia
Gómez, Reyes Víctor Hugo. "Seroprevalencia de brucelosis canina por B. canis en clínicas veterinarias del Gran Santiago 2002-2003." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/133553.
Full textSe muestrearon aleatoriamente 384 caninos de clínicas veterinarias de las 34 comunas del Gran Santiago, determinándose la seroprevalencia de brucelosis canina por Brucella canis. Los animales correspondieron a mayores de seis meses de edad, sin distinción de sexo, raza ni condición sanitaria. La técnica empleada fue contrainmunoelectroforesis con antígeno LPS-R de Brucella ovis, y se realizó en los Laboratorios de Microbiología de la Facultad de Ciencias Veterinarias y Pecuarias de la Universidad de Chile. Junto a la extracción de muestra sanguínea se realizó una encuesta por animal, para determinar el conocimiento sobre la patología por parte del propietario de la mascota, la existencia o no de sospecha del Médico Veterinario tratante y, antecedentes reproductivos relacionados. Serológicamente se obtuvieron 21 muestras sospechosas, a las cuales no se les realizó seguimiento y por lo tanto fueron descartadas. De las 363 restantes se obtuvieron 16,8% (61/363) resultados positivos y 83,2% (302/363) negativos. Según positividad y sexo, el 19,6% de los positivos correspondieron a machos y el 14,5% a hembras, sin diferencias significativas (p>0.05). Se observó una relación de positividad con la edad de las mascotas, obteniéndose la mayor positividad en el grupo de animales de 3 a 5 años. La sensibilidad del médico veterinario a diagnosticar los positivos fue de 24,5% y la especificidad a establecer los negativos fue de 90%. El valor predictivo entre sospecha clínica del veterinario tratante y seropositividad fue de 34,1%. La encuesta reveló escaso conocimiento de los propietarios sobre brucelosis canina; sólo el 4,2% recordó al menos un signo de la enfermedad. La mayoría de los animales positivos resultaron asintomáticos y dentro de los signos clínicos observados, apareció con mayor frecuencia el aborto en hembras y la orquitis en machos. Se destaca la presencia de animales menores de 1 año positivos a brucelosis canina y con antecedentes de no presentar cruza previa
Ramírez, Rabanal Julia Nelly. "Seroprevalencia de Toxoplasma gondii en alpacas de cuatro distritos de la provincia de Canchis-Cusco." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2005. https://hdl.handle.net/20.500.12672/1525.
Full textToxoplasmosis is a parasitic zoonosis widely spread in nature and causes of large economic losses in sheep and goat production. This is used as a reference to determine its possible role in the presentation of reproductive problems in South-American Camelids. The objective of the present study was to quantify the seroprevalence of Toxoplasma gondii in alpacas of diverse communities belonging to the districts of Marangani, Pitumarca, Checacupe and San Pablo, located in the province of Canchis, department of Cusco. A total of 272 blood samples were collected, in march 2003, for the detection of antibodies anti - T. gondii. The used proof was the indirect immunofluorescence test (IFAT), finding a moderate seroprevalence of 35.7 ± 5.7 % (97/272). There were no association among the following variables: district, sex, breed and IFAT response. However it was association between the age and the IFAT response and the percentage of reagent alpacas was increased of the animal matures. Animals of 2 years of age where presented 20.0 ± 12.4 % (8/40), animals of 4 years of age 33.8 ± 11.5 % (22/65) and animals of 6 years or older 40.1 ± 7.4 % (67/167). The results obtained in alpacas of communities agree with other studies already found in South- American Camelids in different zones of the south of Peru.
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Santos, Sánchez Yvan Roger. "Seroprevalencia de leptospirosis en alpacas de la localidad de Quimsachata-Puno en época de lluvias." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2006. https://hdl.handle.net/20.500.12672/678.
Full text--- The Leptospirosis is a zoonotic infections disease, affecting wild and domestics animals and human beings, caused by the Leptospira interrogans. The objective of this study was to estimate the prevalence of leptospirosis in the alpacas during the period of rains. With such an intention were analyzed by means of microaglutination tests, serum of 344 animals of C.I.P. Quimsachata - E.E. ILLPA - INIA - Puno, evaluating the association of the sex and age with the reaction of the test. Of the serum samples analyzed, the 44,77% (154/344) was positive. The detected serovars were pomona, icterohaemorrhagiae, and canicola, not being antibodies against hardjo. Of the 4 studied serovars, pomona showed the highest prevalence 43,60% (150/344), followed by icterohaemorrhagiae with 9,88%(34/344) and canicola with 1,45%(5/344), not finding positive samples in the young animals, whereas for the adults was 60,98 % in males and 74,36 % in females. Relating the positive serum to the sex, there exists absence of statistical significance (p> 0.05) indicating independence, and considering the age, statistical association exists (p menor a 0,05). The high titles found for pomona and icterohaemorrhagiae in these animals, suggest a strong active infection during the period of rain. Key Words: leptospirosis, alpacas, seroprevalence.
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Valdivia, Pabón Lesmes Helbert. "Seroprevalencia de Brucella sp. en bovinos criollos de crianza extensiva de la provincia de Parinacochas, Ayacucho." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2002. https://hdl.handle.net/20.500.12672/3235.
Full text--- The objetive of the present study was to determine the seroprevalence of brucella sp. In pastured Creole cattle of the province of Parinacochas, departament of ayacucho. To this end bovine serum samples were obtained, amog cows and heifers from 24 herds (n= 385), from the 4 districts with the largest bovine population: Chumpi, Cora cora, puyusca and Pullo, to detection of antibodies against Brucella sp. By Rose Bengal agglutination test. Brucella sp. Sero reactor animals were not found, wich suggest absent of Brucella sp. Infection or if it occurred, it would have a prevalence of Brucelosis in the area sampled may be due to the effective surveillance made by the sanitary authorities on internal movement of animals, as well as the weather, kind of breeding, wich may be adverse factors for Brucella sp. Infection to spread among the animals from the districts sampled.
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Soto, Soto Gabriel Angel. "Evaluación de la seroprevalencia y estado de infección por Toxoplasma gondii en gatos de Lima Metropolitana." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2019. https://hdl.handle.net/20.500.12672/10405.
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Castillo, Laura Helen. "Rol de la infección palúdica durante el embarazo sobre la enfermedad de chagas de la madre y del niño en el hospital Materno Infantil de la ciudad de Yacuiba en el departamento de Tarija en los años 2004 al 2005." Universidad Mayor de San Andrés. Programa Cybertesis BOLIVIA, 2007.
Find full textGómez, Oré Felices Rosas. "Determinación de la seroprevalencia de la toxoplasmosis en alpacas y llamas en la estación experimental Inia-Puno." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2002. https://hdl.handle.net/20.500.12672/1238.
Full textThe objetive of this study was to determine the seroprevalence of Toxoplasma gondii in alpacas and llamas from the experimental station of INIA (Quimsachata) Puno, placed in the district of Santa Lucía, province of Lampa, Puno. To this end, they were gathered in June of 2000, blood serum of 200 alpacas and 136 llamas among females and males, to detection of antibodies by Indirect Hemaglutination (IHA). 44.50±6.89% (89/200) of the samples from alpacas showed antibodies with tittles that varied from 1/16 to 1/512. Seroprevalence found in females 56± 9.73% (30.88/100) was bigger than that in males 33± 9.22% (25/100), which increased as age range of animals increased, thus in age ranges from birth to 1 year, more than 1 to 2 years, more than 2 to 3 years and more than 3 years, seroprevalence was 30±12.70%, 38±13.45%, 48±13.85% and 62±13.45% respectively. 27.94±7.54% (38/136) of the samples from llamas showed antibodies with tittles which varied from 1/16 to 1/256. Seroprevalence found in females 30.88± 10.98% (56/100) was similar to that in males 25± 10.29% (33/100), which increased as the age range of animals increased, thus in age ranges from birth to 1 year, more than 1 to 2 years, more than 2 to 3 years and more than 3 years seroprevalences was 0%, 26.4741.38%, 38.24± 16.34% and 47.06±16.78% respectively. The result of this research showed seroprevalence relatively higher in alpacas than that in llamas in INIA station, Puno. It was found that sex represents a risk factor of acquiring toxoplasmosis in alpaca, however it does not occur the same in llamas. It was found that the age variable is a risk factor for the infection with Toxoplasma gondii in llamas. Moreover for the infection with Toxoplasma gondii in llamas. Moreover age constitutes a risk factor of acquiring toxoplasmosis in llamas (p<0.0001)Key words: Toxoplasma gondii, seroprevalence, IHA, alpacas, llamas, Puno.
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Cornejo, Prado Nathann Jesús. "Seroprevalencia de N. caninum en perros de establos lecheros de la cuenca izquierda del Valle del Mantaro." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2004. https://hdl.handle.net/20.500.12672/1468.
Full textThe neosporosis is an emergent disease, considered important cause of abortion in the cattle and serious neuromuscular disease in dogs. The aim of the present study was to determine the seroprevalencia of Neospora caninum in dogs of dairy farms of the left basin of the Valley of Mantaro. 124 serum of dogs were evaluated of 24 dairy farms of the provinces: Huancayo, Jauja and Concepcion. A prevalencia of antibodies against N. caninum dectect was 19.35 ± 6.95 % (24/124), in a dilution of 1:50, by means of the indirect inmunofluorescense test. The percentage of stables that possessed at least an infected dog was 62.5 % (15/24). Association were not situated between the rate of infection and the variables geographical location, age, gender, and origin (of the stable or of around). This result shows, at dogs in the Valley of the Mantaro, a seroprevalence of N. caninum moderate, by what there is recommended the control of the aproach of the dogs for the dairy establishments, moreover that infection is present in the majority of stables in the Valley of the Mantaro.
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Llanco, Albornoz Luis Antonio. "Seroprevalencia y riesgo de infección de leptospirosis bovina en una localidad de costa y otra de sierra." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2007. https://hdl.handle.net/20.500.12672/4960.
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Oré, Ramos Brunella, and Espinoza Alvaro Patiño. "Factores asociados a los diferentes patrones de marcadores para Virus de la Hepatitis B en pacientes peruanos viviendo con VIH, 2016-2017." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2021. http://hdl.handle.net/10757/655170.
Full textIntroduction: Hepatitis B virus (HBV) and HIV Co-infection affects 2.7 million people, which is equivalent to 5-10% of people living with HIV. This comorbidity changes the natural history of both pathologies. In Peru, the characteristics of HIV patients according to the different serological patterns of HBV are not well defined. Methods: Secondary analytical cross-sectional study of a database obtained by reviewing medical records in two public hospitals in Lima, Peru. People aged 18 years or older with a confirmed diagnosis of HIV who started/restarted highly active antiviral treatment (HAART) 2016-2017 were included. Surface antigen (HBsAg), Anti-surface antigen (anti-HBs), Anti-core antigen, and serological patterns according to these markers were evaluated. Crude and adjusted Prevalence Ratios (aPR) were obtained for the different characteristics using Poisson regression with robust variance. Results: 429 patients were included with a prevalence of HBsAg of 4.43% (2.69% -6.83%). There was significant association between HBsAg prevalence and intravenous drugs use (aPR: 1.70; 95% CI 1.07-2.68), transaminasemia (aPR: 3.69; 95% CI 2.97-4.57) and prior HAART use (aPR: 0.52; 95% CI 0.35-0.77). 129 patients without active infection were evaluated for anti-HBs, with 8.53% (4.33% -14.74%) being positive. Association was found with age ≥30 years (aPR: 5.09; 95% CI 2.72-9.54), previous HAART use (aPR: 3.01; 95% CI 1.84-4.93) and viral load ≥1000 copies / ml (aPR: 0.29; 95% CI 0.13-0.63). Conclusions: Intravenous drug use and transaminasemia were associated with a higher prevalence of active infection (HBsAg +), while previous use of HAART showed a lower prevalence. Age ≥30 years and HAART were associated with a higher prevalence of anti-HBs in patients without active infection, while viral load ≥1000 copies/ml was associated with lower prevalence.
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Zuzunaga, Dávalos Mijail Heckla. "Seroprevalencia de Toxoplasma gondii en vicuñas de la Reserva Nacional de Pampa Galeras-Proyecto San Cristóbal y aledaños." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2006. https://hdl.handle.net/20.500.12672/670.
Full text--- Toxoplasma gondii is a protozoan parasite, which definitive host is the feline and has a wide range of species like sheep, goats, pigs, birds and humans as intermediate hosts. This protozoan is worldwide distributed and causes reproductive problems in cattle, specially sheep. Its importance takes root in its zoonótica implication. Studies performed in wild South American camelids like the vicunas are scarce, specially those directed to determine reproductive and epidemiological problems. The objective of this study was to determine the Seroprevalence of T. gondii in vicunas of the National Reserve of Pampa Galeras in the department of Ayacucho. Therefore, 191 serum of vicunas were evaluated, male and female, by using the inmunofluorescent antibody test (IFAT). The Seroprevalence obtained was 5.8 % +/- 3.3. Significant statistical differences were not observed in the results, comparing the variables age and sex. The present study demonstrates that infection of T. gondii in vicunas of the National Reserve of Pampa Galeras occurred. Key Words: Toxoplasmosis, wild SC , serology, antibodies, Ayacucho.
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Aguilar, Salomón Renzo Edward. "Seroprevalencia del virus de la diarrea viral bovina en ganado lechero de crianza intensiva del valle de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2006. https://hdl.handle.net/20.500.12672/5440.
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Fernández, Rojas Milagros Esthefany. "Determinación de la seroprevalencia de Toxoplasma gondii en caprinos destinados al consumo humano en el departamento de Piura." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2021. https://hdl.handle.net/20.500.12672/17522.
Full textPerú. Universidad Nacional Mayor de San Marcos. Proyecto con financiamiento para grupos de investigación 2019 del Vicerrectorado de Investigación y Posgrado. A19080541
Sánchez, Vega Francisco Javier. "Seroprevalencia de brucelosis canina en perros con dueño del gran Santiago y factores de riesgo asociados a su presentación." Tesis, Universidad de Chile, 2018. http://repositorio.uchile.cl/handle/2250/151069.
Full textSe determinó la seroprevalencia de brucelosis canina, producida por Brucella canis, en perros con dueño, con el fin de conocer la realidad de esta infección en el Gran Santiago. Para ello se muestrearon 449 perros pertenecientes a las 34 comunas que lo conforman, de ambos sexos y mayores a 6 meses de edad, los cuales fueron distribuidos por zonas (centro, norte, sur, oriente, poniente). A estos perros, se les extrajo una muestra sanguínea, las cuales se analizaron a través de la técnica de ELISA indirecto, utilizando como antígeno un lipopolisacárido rugoso (LPSR) de Brucella abortus RB51. Junto con la obtención de las muestras, se realizó una encuesta, con el fin de asociar la positividad a la infección de B. canis a factores de riesgo, mediante un modelo de regresión logística múltiple. Se obtuvieron 40 muestras positivas, lo que representa una seroprevalencia corregida por estrato de 8,7%; EE= 2,8% (IC: 5,9% - 11,5%) en el Gran Santiago, la mayoría provenientes de centros de esterilización municipales (74% del total), siendo la zona sur y poniente las de mayor importancia. De las 449 muestras totales, sólo 392 muestras fueron consideradas para identificar los factores de riesgo, ya que las 57 muestras restantes no presentaron información sobre la variable “Número de cruzas que ha tenido”. Las variables independientes “Edad (en años)” (O.R.= 1,18), “Total de perros que viven juntos” (O.R.= 1,12) y “Número de cruzas” (O.R.= 1,37) fueron significativas y consideradas factores de riesgo. Con los resultados de este estudio se concluye que el 8,7% de los perros con dueños del Gran Santiago se infectaron con Brucella canis, infección que se asoció positivamente a la edad, al número de perros que cohabitan y al número de cruzas, lo que recalca la importancia que implica el control de brucelosis canina por B. canis en salud pública, principalmente a través de la esterilización temprana de las mascotas, por ser una enfermedad zoonótica y por el hecho de que las muestras se obtuvieron de pacientes clínicamente sanos.
Rojas, Mendoza Efraín. "Seroprevalencia de cisticercosis en pacientes de consulta externa del Hospital Hugo Pesce Pescetto de Andahuaylas – Apurímac, mediante Western Blot." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2013. https://hdl.handle.net/20.500.12672/14240.
Full textEvalúa la seroprevalencia de cisticercosis en pacientes de consulta externa del Hospital Hugo Pesce Pescetto de Andahuaylas – Apurímac por Western blot. Se obtuvo una muestra de sangre, previo consentimiento informado, de 42 pacientes mayores de 20 años durante enero a noviembre del 2011. Se utilizó el kit Cistiblot, que incluye un control positivo con 9 bandas proteicas. Se realizó un estudio descriptivo transversal. Los datos fueron procesados en SPSS.19 con un análisis estadístico descriptivo. Se determinó 9,5% (4/42) de casos positivos a Cisticercosis, presentando de 2 a 8 bandas proteicas. La edad promedio de los pacientes fue 32,5±7,9 años, correspondiendo un 75% (3/4) al sexo femenino. Dentro de las características socio culturales encontradas tenemos: viviendas precarias (adobe 61,9%, techos de calamina 57,1%, piso de tierra 54,8%), hacinamiento (100%); Dentro de la sintomatología clínica encontramos: dolor de cabeza (100%), insomnio (75%), dolor abdominal (75%) y mareos (75%). Con costumbres alimenticias inadecuadas: consumo de verduras y frutas sin lavar (64,3%) y carne de cerdo mal cocida (19%), crianza de animales (78,9 %) y malos hábitos higiénicos. Todos estos factores permitieron la ingesta accidental de los huevos de la Taenia solium, siendo la principal causa de riesgo para esta zoonosis.
Tesis
Luyo, Avila Christian Felix. "Factores asociados a la seroprevalencia de Toxoplasma gondii en cerdos de granjas tecnificadas y no tecnificadas de Lima-Perú." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2015. https://hdl.handle.net/20.500.12672/4608.
Full text--- The study objective was to determine the seroprevalence of Toxoplasma gondii in pigs from well managed and poor managed farms of the Lima, Peru and identify factors associated with transmission. The work was done in 407 animals from 7 farms whit pig well managed and 10 poor managed located in the coastline of the department of Lima. Epidemiological surveys were applied on farms to identify potential factors associated with the transmission of T. gondii in pigs. Subsequently blood samples were collected from pigs in the finishing phase from well managed (264) and poor managed (143), Serum samples were preserved frozen (-70 ° C) until processing in the Laboratory of Parasitology, Faculty of Veterinary Medicine of UNMSM. To diagnose of swine toxoplasmosis was used the indirect technique ELISA. The association between seroprevalence of T. gondii and each of the evaluated variables (sex, provenance, animal density, water source, type of accommodation, presence of cats and rodent control) were analyzed mediantes multiple models of logistic regression. The seroprevalence of T. gondii in pig of the well managed farms and poor managed farms from Lima-Peru is 4.5 and 33.6% respectively. Factors associated with the transmission of T. gondii in pigs are the provenance (OR: 12.31), water source (OR: 6.44), type of accommodation: mixed (OR: 6.14) and not stabled (OR: 13.59); the presence of felines: 1 to 3 (OR: 5.29) and ≥4 (OR: 16.02); and the rodent control (OR: 7.81). KEYWORDS: swine, Toxoplasma gondii, indirect ELISA, logistic regression
Tesis
OJEDA, CARRASCO JUAN JOSE 360202, and CARRASCO JUAN JOSE OJEDA. "Estimación de las pérdidas económicas por abortos en la lechería en pequeña escala en el sur oriente del Estado de México." Tesis de doctorado, Universidad Autónoma del Estado de México, 2013. http://hdl.handle.net/20.500.11799/63965.
Full textConsejo Nacional de Ciencia y Tecnología (CONACYT), 3161/2012 ESP
Castillo, Vera Felipe Ignacio. "Determinación de la Seroprevalencia de Mycoplasma gallisepticum y Mycoplasma synoviae en una empresa de aves de postura de múltiples edades." Tesis, Universidad de Chile, 2014. http://repositorio.uchile.cl/handle/2250/145139.
Full textSe recolectaron 453 muestras de suero de gallinas ponedoras pertenecientes a una empresa productora de huevos ubicada en la Región de Valparaíso, y se determinó la seroprevalencia de Mycoplasma gallisepticum (MG) y Mycoplasma synoviae (MS) a través de la detección de anticuerpos utilizando la prueba de ensayo por inmunoabsorción ligado a enzimas (ELISA). Las muestras se obtuvieron desde los 10 sectores con aves de diferentes edades pertenecientes a las 4 granjas que componen la empresa, y se evidenció que todos éstos presentaron individuos positivos a MG y/o MS. Del total de las 453 muestras de sueros, se observó que 239 (52,8%) eran positivas a MG, mientras que 322 (71,1%) eran positivas a MS. Los rangos de positividad fluctuaron entre un 6,7% y un 100% tanto para MG como para MS en los distintos sectores evaluados. Para ambos agentes se evaluaron diferencias en los factores grupo etario, tipo de galpón, granja y sector productivo. En el caso de MG, la granja Los Ceibos, los sectores Ceibo 1, Ceibo 2 y Ceibo 3, y los galpones abiertos, fueron los que mostraron una mayor cantidad de muestras positivas. Mientras que para MS, la granja Los Ceibos, los sectores Ceibo 1, Ceibo 3, Los Boldos y Los Espinos, los galpones abiertos y los grupos etarios de mediana y avanzada edad fueron los que mostraron una mayor cantidad de muestras positivas.
453 serum samples from laying hens belonging to an egg producer located in the Valparaíso region were collected to determine the seroprevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) by using the enzyme-linked immunosorbent assay (ELISA). The samples were obtained from 10 sectors of the company, which had birds of different ages belonging to the company 4 farms. It was shown that all these sectors have positive MG and / or MS birds. Of the total of 453 serum samples 239 (52.8%) were positive to MG, while 322 (71.1%) were positive to MS. The ranges of positivity ranged between 6.7% and 100% for both MG and MS in the different sectors evaluated. Differences in certain factors were evaluated for both agents: age, type of barn, farm and production sector. For MG, the farm Los Ceibos, the Ceibo 1 sector, Ceibo 2 sector, Ceibo 3 sector and open sheds showed a greater number of positive samples. For MS, the farm Los Ceibos, the Ceibo 1 sector, Ceibo 3 sector, Los Boldos sector, Los Espinos sector, open sheds and the age groups of middle and old age were those who had a higher number of positive samples.
Whittembury, Vlásica Alvaro Manuel. "Seroprevalencia de hepatitis B y factores asociados en gestantes y su pareja de 6 pueblos indígenas amazónicos. Perú, 2007-2008." Master's thesis, Universidad Nacional Mayor de San Marcos, 2011. https://hdl.handle.net/20.500.12672/15812.
Full textDetermina la magnitud de la infección por hepatitis B, así como los factores asociados en gestantes indígenas de la Amazonía y su pareja masculina. El estudio es analítico, observacional, transversal. Participaron mujeres gestantes y sus parejas masculinas de 6 pueblos indígenas de la Amazonía peruana, Shipibo Konibo, Ashaninka, Candozi, Shapra, Chayahuita y Matsigenka. Se obtuvo muestras de suero y se encuestó a las gestantes y sus parejas entre junio 2007 a diciembre 2008. Las muestras se procesaron con pruebas de ELISA para anticuerpos contra el antígeno core de la hepatitis B (anti-HBc) y el antígeno de superficie de la hepatitis B (HBsAg). Asimismo, se obtuvo información con respecto a variables sociodemográficas y de comportamiento sexual. De 1251 gestantes y 778 parejas masculinas aceptaron participar en el estudio. La seroprevalencia de anti-HBc en gestantes fue de 42.06% (39.30% – 44.87%) y en las parejas masculinas de 54.09% (50.31% -57.84%). La seroprevalencia de HBsAg en gestantes fue de 2.80% (1.41% – 4.95%), y en sus parejas masculinas 4.81% (2.72% – 7.81%). La positividad a anti-HBc en las gestantes estuvo asociada a 1) edad (gestantes-cuantitativa): OR=1.06 (1.03 – 1.09; p<0.001); 2) Inicio de actividad sexual antes de los 16 años de edad: OR=1.86 (1.11 – 3.11; p=0.018); 3) Pareja masculina con anti-HBc(+): OR=6.85 (4.60 – 10.18; p<0.001); 4) Pareja masculina con contacto sexual con homosexual: OR=3.24 (1.29 – 8.12; p=0.012); 5); Uso de condón por pareja masculina: OR=0.58 (0.38 – 0.90; p=0.014). La positividad a anti-HBc en las parejas masculinas estuvo asociada a: 1) Gestantes anti-HBc (+): OR=6.41 (4.49 – 9.17; p<0.001); 2) gestante con inicio de actividad sexual antes de los 16 años de edad: OR=1.62 (1.03 – 2.56; p=0.038); 3) Edad pareja masculina (cuantitativa): OR=1.04 (1.02 – 1.06; p=0.000); 4) Pareja masculina con contacto sexual con homosexual: OR=3.95 (1.38 – 11.27; p=0.010). Se concluye que la seroprevalencia de anti-HBc de 42.06% en gestantes y de 54.09% en sus parejas, indican que la Hepatitis B es una enfermedad hiperendémica en las poblaciones indígenas estudiadas. Los factores asociados sugieren que la vía sexual es la principal vía de transmisión de esta enfermedad en la población en edad reproductiva.
Manrique, Asto Emma Nancy. "Seroprevalencia de virus linfotrópico HTLV1 en pacientes sintomáticos en el Hospital Nacional Daniel Alcides Carrión periodo enero 2008-diciembre 2012." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2014. https://hdl.handle.net/20.500.12672/13138.
Full textTrabajo académico
Navarro, Mamani Dennis Alexander. "Factores de riesgo asociados a la seroprevalencia de Toxoplasma gondii en mamíferos del orden carnívora y primate mantenidos en cautiverio." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2014. https://hdl.handle.net/20.500.12672/3602.
Full textTesis
Saravia, Palomino Marco Antonio. "Seroprevalencia de Toxoplasma gondii en llamas hembras adultas en la U.P. Alianza-Antacalla de la E.P.S. Rural Alianza Melgar - Puno." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2004. https://hdl.handle.net/20.500.12672/2264.
Full textThe objective of the present work was to determine the seroprevalence of Toxoplasma gondii in llamas adult females of two ends of parturition of the Unit of production Alianza-Antacalla in the Company of social property Rural Alliance in the province of Melgar-Puno, by means of the test of indirect inmunofluorescence; for which 157 of blood samples of llamas were obtained adult females, of them 112 proven of the end of parturition of alliance and 45 of the end of Río Grande. Found that the 10.19± 4.7% (16/157) of the total of llamas adult females they presented antibodies against T. gondii; the seroprevalences found in the ends of Río Grande and Alianza were of 13.33± 9.8% (6/45) and 8.93± 5.3% (10/112) respectively, not found significant statistic difference among them. The seroprevalences of T. gondii for ranks of ages from 2 to 3, 4 to 5 and greater or equal to 6 ages, were of 9.09± 8.5, 15.38± 13.87 and 9.19± 6.07 respectively; not presenting significant statistic difference between ages groups had possibly to that these animals were adult. The results of this study show a seroprevalence of T. gondii relatively low in this company, in relation to other camelids in similar studies; moreover we determ that the variables age and end of parturition did not represent factors of risk for infection of T. gondii in llamas adult females.
Tesis
Zacarías, Ríos Erik Alberto. "Seroprevalencia del virus de la rinotraqueitis infecciosa bovina en bovinos criollos de crianza extensiva de la provincia de Parinacochas, Ayacucho." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2002. https://hdl.handle.net/20.500.12672/1562.
Full textThe seroprevalence of Bovine Herpes Virus-1, the causative agent of Infectious Bovine Rinotracheitis (IBR), in criollo bovines from districts of Coracora, Chumpi, Puyusca and Pullo of the Parinacochas Province, Ayacucho was carried out. Four hundred sixty nine serum samples from twenty five herds were tested by virus neutralisation test to detect neutralizing antibodies. The 67.59 ± 4.24% (317/469) of the samples had antibodies against BHV-1. The seroprevalence of the virus was similar in the animals from the 4 district studied. The antibodies titers ranged from 1:2 to> 1:256. All the sampled herds had seroreactive animals. This study report the presence of the BHV-1 in criollos bovines from Parinacochas Province, with a prevalence superior to described in dairy herds of the country.
Tesis
Poma, de la Cruz Edwin Cléver. "Seroprevalencia de toxoplasma gondii en alpacas (Lama pacos) de la Unidad de Producción de Cochas de la SAIS Tupac Amaru." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2003. https://hdl.handle.net/20.500.12672/755.
Full text--- Toxoplasma gondii, causative agent of toxoplasmosis, is an important parasite in animal health because it is one of the major parasitic infections that produce reproductive problems, and also it is an important zoonosis worldwide. The objective of this study was to determine the seroprevalence of antibodies against Toxoplasma gondii in alpacas from the Unit of Production of Cochas of the SAIS Tupac Amaru, located in the district of Canchayllo, Province of Jauja, Departament of Junin, Perú. To this end, sera from 200 alpacas among females and male were gathered in the month of December of 2000, for the detection of antibodies by using the method of Indirect Hemaglutination (IHA). 21 5.64 % (42/200) of the samples showed antibodies, with titles ranging from 1/16 up to 1/1024. The seroprevalence found in males was 26.47 (10.47% (18/64), and it was greater than in females, 18.18 6.57 % (24/132), and for age groups, from the birth until 1 year old, more than 1 up to 2 years old and more than 3 years old; the seroprevalence found was 33.33 16.08%, 15.38 9.8, 20 7.31 respectively. The analysis by using The Logistic regression found that the genus did not represent a risk factor. The age did represent a risk factor, being younger alpacas (8 to 12 months) more susceptible than other age groups. These results are coherent with other studies obtained in different parts of world, however, it is observed that there are differences with recent studies obtained in Peru; this, this could be due to environmental factors, frequency of the definitive host. Prevalence found in the present study was moderate, should be done further studies to determine the real importance of T. gondii in the occurrence of abortion in alpacas.
Tesis
Escano, Lopez Josselyn Antuaneth. "Seroprevalencia del virus de Lengua Azul en caprinos de los departamentos del norte: Tumbes, Piura, Lambayeque, La Libertad y Cajamarca." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2021. https://hdl.handle.net/20.500.12672/17683.
Full textPortocarrero, Miranda Gonzalo Carlos. "Factores de riesgo asociados a la seroprevalencia de Neospora caninum en bovinos naturalmente infectados en Ceja de selva de Oxapampa – Perú." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2013. https://hdl.handle.net/20.500.12672/14460.
Full textDetermina los factores de riesgo asociados a la seroprevalencia de Neospora caninum en bovinos naturalmente infectados de Ceja de selva de Oxapampa-Perú. El trabajo se desarrolló en veinte hatos ganaderos de los distritos de Chontabamba y Oxapampa de la provincia de Oxapampa, departamento de Pasco. Se obtuvieron muestras de sangre por punción directa de la vena yugular y coccígea media en 388 bovinos, las mismas que fueron conservadas con refrigerantes, para su traslado y posterior procesamiento en el Laboratorio de Parasitología FMV-UNMSM. Mediante encuestas se levantó información situacional y epidemiológica de cada rebaño muestreado para identificar los potenciales factores de riesgo. Para determinar la presencia de anticuerpos a N. caninum se utilizó la técnica de Elisa de competencia(cELISA). La asociación entre la seroprevalencia a N. caninum y cada una de las variables evaluadas (grupo etario, procedencia, tamaño del hato, eliminación de restos de animales y nº de perros/hato) fueron analizadas mediante regresión logística, donde valores p<0.05 evidenciaron factores de riesgo.La seroprevalencia de la infección de N. caninum en bovinos de Ceja de Selva, de la provincia de Oxapampa, fue de 18.8 ±3.9% (prevalencia corregida de 18.1 ± 3.8% ). Los factores de riesgo asociados con la seropositividad de N. caninum en bovinos de ceja de selva fueron: Tamaño del hato >100 animales OR 2.89, eliminación de restos de animales al aire libre OR 3.31 y presencia de más de tres perros/hato OR 3.21 (p<0.05). La edad y procedencia de los animales no influyeron en el riesgo de presentación de N. caninum (p>0.05).
Tesis
Ayvar, Polo Viterbo. "Seroprevalencia de cisticercosis porcina en las villas de Nueva Esperanza, Matapuquio y Turpo en la provincia de Andahuaylas, departamento de Apurímac." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2002. https://hdl.handle.net/20.500.12672/963.
Full textPorcine cysticercosis is endemic in rural communities of Peru, due to the conditions that contribute to its onset, transmission and maintenance. This thesis assessed the real prevalence of porcine cysticercosis in three villages of Andahuaylas province, Apurimac Department. The entire porcine population was sampled (N = 304), except for suckling piglets under 2 months old and pregnant sows. The samples were examinee by the Enzyme Immuno Electro Transfer Blot (EITB) test. Resulting data was analyzed to determine the risk factors and was analyzed in a stocastic simulation to asses the real prevalence. This stocastic simulation used random numbers generation functions that followed beta binomial distributions. This simulation was implemented in Microsoft Excel 2000® while the functions were implemented using the commercial simulation software @Risk 4.0® from Palisade Corp. the infection level in Nueva Esperanza was significantly lower to those founded in Turpo and Matapuquio (p<0.05), it was also found that a bigger proportion of male pig infected, but it was not estatistically significant. In what respects to the age, a perfectly direct correlation was found between the animals age and the porcine cysticercosis prevalence. The logistic regression showed that the variables sampling location and age represent risk factors asociated with the disease (p<0.0001). Following the same criterion it was observed that the chance to find an cysticercotic pig is higher when the age of the pig raises. According to the serologic sampling results, the general prevalence ratio was 47.3±5.6 while the prevalences for each village were 26.5±7.6 for Nueva Esperanza, 72.3±9.5 for Turpo and 54.0±10.5 for Matapuquio. In what respects to in the simulation results, it was found that the real prevalence for the three villages under study would be of 23% and that the 96% of the happenings would be inside the 17% and 29% interval. It is concluded then that, porcine cysticercosis in the Andahuaylas’ villages is a public health problem.
Tesis
Herrera, Rosalino Antonio. "Seroprevalencia del virus de la diarrea viral en bovinos de crianza extensiva de la provincia de San Pablo, departamento de Cajamarca." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2009. https://hdl.handle.net/20.500.12672/664.
Full textThe aim of this study was to determine the seroprevalence of Bovine Viral Diarrhea Virus (BVDV) in bovines of extensive rearing without history of vaccination, from the province of San Pablo, department of Cajamarca. There were collected 385 blood serum samples of bovines divided in the following age groups: two to younger than six, six to younger than 12 months and older than 12 months of age. The detection of antibodies against BVDV was made through the viral neutralization test. The 27.1 ± 4.4% (104/385) of sampled bovines showed antibodies against BVDV, distributed into the three age groups; there was not statistical difference (p>0.05). The percentage of seroreactors females was 27.8 ± 4.5% (79/285) and males was 25.1 ± 4.3% (25/100); there was not statistical difference (p>0.05). The titles of antibodies were between 2 to higher than 256. The 60.3 ± 9.4% (50/83) of animals older than 12 months presented titles of antibodies between 128 and higher than 256. In conclusion, BVDV is presented on the population of sampled bovines from the province of San Pablo, although with a lower prevalence compared with similar studies made in other areas of the country.
Tesis
Pérez, Montero Xochitl Alondra. "Puesta a punto de una prueba serológica para estudios de seroprevalencia de babesiosis bovina utilizando proteínas recombinantes de Babesia bigemina como antígenos." Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2019. http://hdl.handle.net/20.500.11799/105738.
Full textRESUMEN El presente trabajo fue realizado en el laboratorio de Babesiosis del Centro Nacional de Investigaciones Disciplinarias en Salud Animal e Inocuidad del Instituto Nacional de Investigaciones Agrícolas y Pecuarias (CENID-SAI INIFAP), el objetivo fue poner a punto el Ensayo por Inmunoadsorción Ligado a Enzimas Indirecto (iELISA) para estudios serológicos de babesiosis bovina con el uso de proteína recombinante RAP-1 como antígenos para Babesia bigemina, mediante la inoculación de esta proteína en dos ovinos, con el fin de observar reconocimiento de la proteína a nivel inmunológico (formación de inmunocomplejos), ya que la enfermedad es específica de bovinos. Se realizaron seis inoculaciones para un ovino y siete para el otro, con espacio de 14 días entre inoculaciones a una concentración total de 1900μl/ml junto con adyuvante de Freund vía intramuscular, se extrajeron muestras sanguíneas de cada día que se administró la proteína y se centrifugaron en el Laboratorio de babesiosis del CENID-SAI del INIFAP para obtención de suero sanguíneo y la posterior realización de pruebas serológicas; las cuales fueron Inmunofluorescencia Indirecta (IFI) para la observación microscópica por epifluorescencia de la reacción antígeno nativo–anticuerpo reconocidos en las muestras (considerada como “la prueba de oro” en babesiosis bovina) y el Ensayo por Inmunoadsorción Ligado a Enzimas indirecto (iELISA), para poder identificar reconocimiento antígeno recombinante–anticuerpo. Los resultados obtenidos para la técnica de IFI demostraron positivas las muestras a partir del segundo muestreo para un ovino y partir del tercer muestreo. En la prueba de iELISA resultaron positivos a partir del segundo muestreo para ambos ovinos. De esta forma se demostró que existe reconocimiento inmunológico en ambas técnicas y que los productos derivados de las inoculaciones de la proteína recombinante RAP-1 de Babesia bigemina a ovinos fueron anticuerpos policlonales adquiridos exitosamente. Por otro lado, se determinó la concordancia entre ambas pruebas serológicas para medir el grado de acuerdo entre ellas (precisión) obteniendo una concordancia sustancial. Posteriormente se realizaron pruebas serológicas de un hato proveniente de una zona endémica para comprobar que el antígeno recombinante puede reconocer para el antígeno completo de B. bigemina de campo. Del mismo modo se realizó la prueba de Inmunofluorescencia indirecta para confirmar el reconocimiento de anticuerpos y determinar que efectivamente el ganado estuvo en contacto con los antígenos, posteriormente se realizó Ensayo por Inmunoadsorción Ligado a Enzimas, para determinar que el antígeno recombinante RAP-1 de Babesia bigemina es capaz de reconocer al antígeno completo de Babesia bigemina. Los resultados demostraron que el antígeno recombinante RAP-1 reconoce al anticuerpo completo de Babesia bigemina, siendo de utilidad para estudios epidemiológicos de babesiosis bovina. Palabras clave: Babesia bigemina, RAP-1, reconocimiento, IFI, ELISA.
Forma parte de un proyecto con financiamiento del CONACYT
Palacios, Cárdenas Miguel. "Puesta a punto de una prueba serológica para estudios de seroprevalencia de babesiosis bovina utilizando proteínas recombinantes de Babesia bovis como antígenos." Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2019. http://hdl.handle.net/20.500.11799/105743.
Full textEl presente trabajo fue realizado en el laboratorio de Babesiosis del Centro Nacional de Investigaciones Disciplinarias en Salud Animal e Inocuidad del Instituto Nacional de Investigaciones Agrícolas y Pecuarias (CENID-SAI INIFAP), el objetivo fue poner a punto el Ensayo por Inmunoadsorción Ligado a Enzimas Indirecto (iELISA) para estudios serológicos de babesiosis bovina con el uso de proteína recombinante MSA-1 como antígenos para Babesia bigemina, mediante la inoculación de esta proteína en dos ovinos, con el fin de observar reconocimiento de la proteína a nivel inmunológico
Proyecto No. 1-1.6-1373533989-P-M.2-2 "Desarrollo de pruebas de diagnostico rápido para la babesiosis bovina” del INIFAP a cargo del Dr. Julio Vicente Figueroa Millán
Gómez, Marín Ángel. "Seroprevalencia del virus de la artritis encefalitis caprina en cabras de la provincia de Lima, Canta, Huaura y Huaral del departamento de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2014. https://hdl.handle.net/20.500.12672/4180.
Full textTesis
Santome, Tolentino Elva Isela. "Principales factores de riesgo y la seroprevalencia de hepatitis C en donantes del banco de sangre del Hospital Nacional Hipólito Unanue durante el 2005." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2007. https://hdl.handle.net/20.500.12672/14746.
Full textTrabajo académico
Cabellos, Rojas Karina Olinda. "Seroprevalencia de los virus : parainfluenza 3, respiratorio sincitial, diarrea viral bovina, en un rebaño mixto de una comunidad campensina de la provincia de Calca, Cusco." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2006. https://hdl.handle.net/20.500.12672/684.
Full text--- The seroprevalence of Bovine Viral Diarrhoea Virus (BVDV), Parainfluenza Virus Type 3 (PI3V) and Respiratory Syncytial Virus (RSV) in serum samples of alpacas (n igual 21), bovine (n igual 66) and sheep (n igual 152) of a rural community of Cusco, Peru. It was carried out by virus-neutralization test. The 15.8±16.4% (3/21), 4.8 ±9.1% (1/21) and 23.8±18.2% (5/21) of alpacas had neutralizing antibodies against BVD, RS and PI3 virus. The 90.9±6.9% (60/66), 87.88±7.9% (58/66) and 81.8 ±9.3% (54/66) of bovine and the 28.29±7.1% (43/152), 49.34 ±7.9% (75/152) and 50.0 ±7.9% (76/152) of sheep had antibodies to BVDV, RSV and PI3V respectively. These results confirm the presence of viral infections in ruminants of mixed breeding system of a rural community.
Tesis
Ramos, Miraval Rocío del Rosario. "Seroprevalencia y factores de riesgo de hepatitis B y C en donantes de banco de sangre del Hospital Naval, enero de 1999 - abril de 2004." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2005. https://hdl.handle.net/20.500.12672/1870.
Full textThe aim of the present work was to determine the seroprevalence of the virus of the hepatitis C (VHC) and of the hepatitis B (VHB) in military and civil donors who came to the Bank of Blood of the Navy Hospital as well as the associate factors of risk. It is a question of a transverse, descriptive and retrospective study realized in the Bank of Blood, which information epidemiological and proved from the general tamizaje was obtained of the Books of records of the Bank of Blood and Clinical Histories in case of the military donors, in the included period of January, 1999 until April, 2004. Murex’s Test HBsAg Version 3 and Hepanostika Anti-HBc UniForm were used for Hepatitis B, whereas for the virus of the Hepatitis C there was in use Elisa's Test of third generation. A whole of 7009 donors was registered between 1999 and 2004.Were included in the present study 320, those who were presenting antibodies against the virus C (anti-VHC) and for the virus of hepatitis B: Surface antigen (HBsAg) and/or Antigen Core-IgG. The information was gathered, percentages were obtained and the results were analyzed by means of SPSS 10 Windows version Statistics Program: chi-square or Fisher’s Tests. RESULTS: The prevalence of carriers of VHC and VHB was 0.74% and 0.34%, respectively. The principal infection people are between 18 to 30 years old, and belong to health navy personal for HBV infection and Marine Infantry for HCV infection. It couldn’t find risks factors in relation to the two etiologists, due a negative rate of 96,4%. CONCLUSIONS: The results suggest a low prevalence of infection for VHB but high for VHC infection in the studied population as it are indicate in the national and international reports. The poor or useless association between de risks factors and the illness it could be in relation to a limited effective and a poor utility of de Confidential Autoexclusion Exam. It’s necessary that this exam must be complete and have the property to identify more risks factors on the transmission of HBV and HCV.
Tesis de segunda especialidad
García, Pinto John, and Luna Gerardo Jiménez. "Estudio comparativo sobre la seroprevalencia de HTLV-I en una población de adultos en régimen privado de libertad y una población urbano marginal de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2002. https://hdl.handle.net/20.500.12672/2076.
Full textTesis de segunda especialidad
Salinas, Villaorduña Katherine Rocio. "Seroprevalencia de HTLV 1 y 2 y características epidemiológicas de donantes de sangre seropositivos de un hospital público de Lima - Perú en el año 2018." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2020. https://hdl.handle.net/20.500.12672/11852.
Full textTesis
Choque, Ramos Oscar Raúl. "Seroprevalencia de marcadores infecciosos hemotransmisibles y factores de riesgo asociados en postulantes a donación en el banco de sangre del Hospital María Auxiliadora marzo 2015 – marzo 2016." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2017. https://hdl.handle.net/20.500.12672/7086.
Full textTesis