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Journal articles on the topic 'Sertoli cells'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Xiong, Weipeng, Haikun Wang, Hui Wu, Yongmei Chen, and Daishu Han. "Apoptotic spermatogenic cells can be energy sources for Sertoli cells." REPRODUCTION 137, no. 3 (2009): 469–79. http://dx.doi.org/10.1530/rep-08-0343.

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Apoptotic spermatogenic cells and residual bodies are phagocytosed and degraded by Sertoli cells during mammalian spermatogenesis. The meaning of this event remains to be clarified. In this report, we demonstrate that apoptotic spermatogenic cells and residual bodies can be used to produce ATP by Sertoli cells after phagocytosis of them. Sertoli cells produced the highest level of ATP compared with other testicular cells. Phagocytosis assayin vitroshowed that engulfment of apoptotic spermatogenic cells increases ATP production by Sertoli cells. The increased ATP production was detected in semi

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2

Golat, Brian T., and Don F. Cameron. "Sertoli Cells Enhance Formation of Capillary-Like Structures in Vitro." Cell Transplantation 17, no. 10-11 (2008): 1135–44. http://dx.doi.org/10.3727/096368908787236512.

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Sertoli cells isolated from the testis (referred to as extratesticular Sertoli cells) have been shown to facilitate allo- and xenogeneic cell transplantations. It appears likely that the ability of these cells to enhance the success of cell engraftment is due, in part, to the retention of their intratesticular functions of trophic support and immunoprotection. Sertoli cells also are involved in the regulation of angiogenesis in the testis, which may also contribute to enhanced cell engraftment success facilitated by extratesticular Sertoli cells. Because the maintenance of the cell's intratest

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3

Griswold, Michael D. "50 years of spermatogenesis: Sertoli cells and their interactions with germ cells." Biology of Reproduction 99, no. 1 (2018): 87–100. http://dx.doi.org/10.1093/biolre/ioy027.

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Abstract The complex morphology of the Sertoli cells and their interactions with germ cells has been a focus of investigators since they were first described by Enrico Sertoli. In the past 50 years, information on Sertoli cells has transcended morphology alone to become increasingly more focused on molecular questions. The goal of investigators has been to understand the role of the Sertoli cells in spermatogenesis and to apply that information to problems relating to male fertility. Sertoli cells are unique in that they are a nondividing cell population that is active for the reproductive lif

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Cameron, D. F., J. J. Hushen, T. Dejarlais, et al. "A Unique Cytoplasmic Marker for Extratesticular Sertoli Cells." Cell Transplantation 11, no. 6 (2002): 507–12. http://dx.doi.org/10.3727/000000002783985530.

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In the absence of a definitive cell marker for testis-derived Sertoli cells, their identification in cell culture or in Sertoli cell-facilitated cell transplantation protocols is difficult and limits the creditable evaluation of experimental results. However, the production by prepubertal Sertoli cells of Mullerian inhibiting substance (MIS) presents the possibility of specifically identifying extratesticular Sertoli cells as well as Sertoli cells in situ, by the immunodection of this unique glycoprotein. This study was designed to determine if isolated rat Sertoli cells could be identified by

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Zhang, Lianjun, Min Chen, Qing Wen, et al. "Reprogramming of Sertoli cells to fetal-like Leydig cells by Wt1 ablation." Proceedings of the National Academy of Sciences 112, no. 13 (2015): 4003–8. http://dx.doi.org/10.1073/pnas.1422371112.

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Sertoli and Leydig cells, the two major somatic cell types in the testis, have different morphologies and functions. Both are essential for gonad development and spermatogenesis. However, whether these cells are derived from the same progenitor cells and the mechanism regulating the differentiation between these two cell types during gonad development remains unclear. A previous study showed that overactivation of Ctnnb1 (cadherin-associated protein, beta 1) in Sertoli cells resulted in Sertoli cell tumors. Surprisingly, in the present study, we found that simultaneous deletion of Wilms’ Tumor

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6

Xiong, Weipeng, Yongmei Chen, Huizhen Wang, et al. "Gas6 and the Tyro 3 receptor tyrosine kinase subfamily regulate the phagocytic function of Sertoli cells." REPRODUCTION 135, no. 1 (2008): 77–87. http://dx.doi.org/10.1530/rep-07-0287.

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The apoptotic spermatogenic cells and residual bodies are phagocytosed and degraded by Sertoli cells during spermatogenesis. The mechanisms of this process are largely unknown. Here, we demonstrate that Gas6 and its receptors, the Tyro 3 subfamily of receptor tyrosine kinases (RTKs; Tyro 3, Axl, and Mer), regulate the phagocytic function of Sertoli cells. The phagocytic ability of Sertoli cells increased by five times in the presence of Gas6 in serum-free medium when compared with controls. The Sertoli cells lacking Mer showed a 35% reduction in phagocytosis of apoptotic spermatogenic cells wh

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7

Wang, Shuai, Pengxiang Wang, Dongli Liang, and Yuan Wang. "BRG1 Is Dispensable for Sertoli Cell Development and Functions in Mice." International Journal of Molecular Sciences 21, no. 12 (2020): 4358. http://dx.doi.org/10.3390/ijms21124358.

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Sertoli cells are somatic supporting cells in spermatogenic niche and play critical roles in germ cell development, but it is yet to be understood how epigenetic modifiers regulate Sertoli cell development and contribution to spermatogenesis. BRG1 (Brahma related gene 1) is a catalytic subunit of the mammalian SWI/SNF chromatin remodeling complex and participates in transcriptional regulation. The present study aimed to define the functions of BRG1 in mouse Sertoli cells during mouse spermatogenesis. We found that BRG1 protein was localized in the nuclei of both Sertoli cells and germ cells in

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8

Yokonishi, Tetsuhiro, and Blanche Capel. "Differentiation of fetal sertoli cells in the adult testis." Reproduction 162, no. 2 (2021): 141–47. http://dx.doi.org/10.1530/rep-21-0106.

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Sertoli cells proliferate and construct seminiferous tubules during fetal life, then undergo differentiation and maturation in the prepubertal testes. In the adult testes, mature Sertoli cells maintain spermatogonia and support spermatogenesis during the entire lifetime. Although Sertoli-like cells have been derived from iPS cells, they tend to remain immature. To investigate whether Sertoli cells can spontaneously acquire the ability to support spermatogenesis when transferred into the adult testis, we transplanted mouse fetal testicular cells into a Sertoli-depleted adult testis. We found th

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9

Hadley, M. A., S. W. Byers, C. A. Suárez-Quian, H. K. Kleinman, and M. Dym. "Extracellular matrix regulates Sertoli cell differentiation, testicular cord formation, and germ cell development in vitro." Journal of Cell Biology 101, no. 4 (1985): 1511–22. http://dx.doi.org/10.1083/jcb.101.4.1511.

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Sertoli cell preparations isolated from 10-day-old rats were cultured on three different substrates: plastic, a matrix deposited by co-culture of Sertoli and peritubular myoid cells, and a reconstituted basement membrane gel from the EHS tumor. When grown on plastic, Sertoli cells formed a squamous monolayer that did not retain contaminating germ cells. Grown on the matrix deposited by Sertoli-myoid cell co-cultures, Sertoli cells were more cuboidal and supported some germ cells but did not allow them to differentiate. After 3 wk however, the Sertoli cells flattened to resemble those grown on

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10

THOMPSON, ERIK W., ALAN W. BLACKSHAW, and SAMIR S. RAYCHOUDHURY. "Secreted Products and Extracellular Matrix from Testicular Peritubular Myoid Cells Influence Androgen‐Binding Protein Secretion by Sertoli Cells in Culture." Journal of Andrology 16, no. 1 (1995): 28–35. http://dx.doi.org/10.1002/j.1939-4640.1995.tb01726.x.

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ABSTRACT: Metabolic cooperation mediated by secreted factors between Sertoli cells and peritubular myoid cells has been well documented. We have confirmed that factors secreted by peritubular myoid cells modulate androgen‐binding protein (ABP) secretion by Sertoli cells and shown further that this can also be achieved with peritubular myoid cell extracellular matrix (ECM). While peritubular myoid cell ECM potentiated the stimulatory effect of dibutyryl cyclic AMP on Sertoli cell ABP secretion, secreted factors did not, suggesting that the two components influence Sertoli cells through distinct

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11

Cameron, D. F., A. I. Othberg, C. V. Borlongan, et al. "Post-Thaw Viability and Functionality of Cryopreserved Rat Fetal Brain Cells Cocultured with Sertoli Cells." Cell Transplantation 6, no. 2 (1997): 185–89. http://dx.doi.org/10.1177/096368979700600212.

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Testis-derived Sertoli cells have been used to create an immune “privileged” site outside of the testis to facilitate cell transplantation protocols for diabetes and neurodegenerative diseases. In addition to secreting immunoprotective factors, Sertoli cells also secrete growth and trophic factors that appear to enhance the posttransplantation viability of isolated cells and, likewise, the postthaw viability of isolated, cryopreserved cells (26). It would be beneficial if Sertoli cells could be cryopreserved with the transplantable cell type without deleterious effects on the cells. This repor

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12

Zabludoff, Sonya D., Martin Charron, Joshua N. DeCerbo, Natalia Simukova, and William W. Wright. "Male Germ Cells Regulate Transcription of the Cathepsin L Gene by Rat Sertoli Cells*." Endocrinology 142, no. 6 (2001): 2318–27. http://dx.doi.org/10.1210/endo.142.6.8106.

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Abstract It is well known that male germ cells regulate the steady state levels of numerous transcripts expressed by Sertoli cells. To date, however, there has been no direct test of whether this regulation reflects changes in gene transcription and/or transcript stability. This study used two experimental approaches to test the hypothesis that germ cells regulate transcription of the cathepsin L gene by rat Sertoli cells. We examined this gene because, in vivo, steady state levels of cath L messenger RNA in Sertoli cells change in a stage-specific manner as the surrounding germ cells progress

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Yan, Rong-Ge, Qi-Lin Yang, and Qi-En Yang. "E4 Transcription Factor 1 (E4F1) Regulates Sertoli Cell Proliferation and Fertility in Mice." Animals 10, no. 9 (2020): 1691. http://dx.doi.org/10.3390/ani10091691.

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In the mammalian testes, Sertoli cells are the only somatic cells in the seminiferous tubules that provide structural, nutritional and regulatory support for developing spermatogenic cells. Sertoli cells only proliferate during the fetal and neonatal periods and enter a quiescent state after puberty. Functional evidences suggest that the size of Sertoli cell population determines sperm production and fertility. However, factors that direct Sertoli cell proliferation and maturation are not fully understood. Transcription factor E4F1 is a multifunctional protein that serves essential roles in ce

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14

Tajima, Y., H. Onoue, Y. Kitamura, and Y. Nishimune. "Biologically active kit ligand growth factor is produced by mouse Sertoli cells and is defective in SId mutant mice." Development 113, no. 3 (1991): 1031–35. http://dx.doi.org/10.1242/dev.113.3.1031.

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In order to define the role of Kit ligand (KL) growth factor encoded at the mouse steel (SI) locus in spermatogenesis, we have examined its production in Sertoli cells. As a measure KL growth factor bioactivity, the ability to support proliferation and maintenance of mast cells was used in co-culture with primary mouse Sertoli cells. On the sertoli cells derived from +/+ and Wv/Wv mice, +/+ mast cells proliferated and were supported for more than 2 weeks, but not W/Wv mast cells. In contrast, Sertoli cells from SId/SId mice could not support +/+ mast cell proliferation under similar conditions

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15

RAYCHOUDHURY, SAMIR S., ANGELA F. FLOWERS, CLARKE F. MILLETTE, and MARY F. FINLAY. "Toxic Effects of Polychlorinated Biphenyls on Cultured Rat Sertoli Cells." Journal of Andrology 21, no. 6 (2000): 964–73. http://dx.doi.org/10.1002/j.1939-4640.2000.tb03428.x.

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ABSTRACT: Polychlorinated biphenyls (PCBs) are ubiquitous and persistent environmental contaminants. In mammals, PCBs affect spermatogenesis and may be associated with Sertoli cell changes. Therefore, our aim was to evaluate in vitro toxic effects of hydroxylated PCB (PCB‐22; 2′,3′,4′,5′‐tetrachloro‐4‐biphenylol) and PCB congener (PCB‐77; 3,3′,4,4′‐tetrachlorobiphenyl) on Sertoli cells isolated from 19‐ to 21‐day‐old male rats. Sertoli cells incubated for 24 hours in 10−7 M PCB‐22 and 10−8 M PCB‐77, but not in 0.05% ETOH or 10−7M 17β‐estradiol (E2) showed morphological changes. Sertoli cells d

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16

Camacho-Moll, Maria E., Leendert H. J. Looijenga, Roland Donat, Chitranjan J. Shukla, Anne Jørgensen, and Rod T. Mitchell. "Expression of Intermediate Filaments in the Developing Testis and Testicular Germ Cell Cancer." Cancers 14, no. 22 (2022): 5479. http://dx.doi.org/10.3390/cancers14225479.

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Cytokeratin and desmin expression have been associated with Sertoli cell maturity and the development of testicular germ cell cancer (TGCC). Thus, the present study aimed to characterize the expression of these intermediate filaments in normal testis development and TGCC. Cytokeratin and desmin were determined by immunohistochemistry and immunofluorescence in human fetal, and adult testis and tissue from patients with pre-invasive germ cell neoplasia in-situ (GCNIS) or invasive TGCC. Desmin was expressed in Sertoli cells of the human fetal testis, and the proportion of desmin expressing Sertol

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17

Sun, Hongyan, Guomin Zhang, Fulu Dong, Feng Wang, and Wenguang Cao. "Reprogramming Sertoli Cells into Pluripotent Stem Cells." Cellular Reprogramming 16, no. 3 (2014): 196–205. http://dx.doi.org/10.1089/cell.2013.0083.

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18

Calado, Ana Margarida. "150 anos de investigação após a descoberta de Enrico Sertoli." História da Ciência e Ensino: construindo interfaces 27 (January 5, 2024): 209–20. http://dx.doi.org/10.23925/2178-2911.2023v17esppp209-220.

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Resumo A complexa morfologia das células de Sertoli, bem como as suas interações com as células germinativas tem sido foco de investigação desde que a sua descrição pela primeira vez em 1865 por Enrico Sertoli. Nos últimos anos, a investigação no âmbito das células de Sertoli vai muito além da morfologia, da fisiologia e tornou-se cada vez mais focada nas questões moleculares. O objetivo dos investigadores tem sido entender o papel das células de Sertoli na espermatogénese e aplicar essa informação em problemas relacionados à fertilidade masculina. Os numerosos e distintos complexos juncionais

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19

Thomas, Phillip A., Eric D. Schafler, Sophie E. Ruff, Maud Voisin, Susan Ha, and Susan K. Logan. "UXT in Sertoli cells is required for blood–testis barrier integrity†." Biology of Reproduction 103, no. 4 (2020): 880–91. http://dx.doi.org/10.1093/biolre/ioaa121.

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Abstract Spermatogenesis is a complex process that establishes male fertility and involves proper communication between the germline (spermatozoa) and the somatic tissue (Sertoli cells). Many factors that are important for spermatozoa production are also required for Sertoli cell function. Recently, we showed that the transcriptional cofactor ubiquitously expressed transcript (UXT) encodes a protein that is essential in germ cells for spermatogenesis and fertility. However, the role of UXT within Sertoli cells and how it affects Sertoli cell function was still unclear. Here we describe a novel

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Wang, Huizhen, Haikun Wang, Weipeng Xiong, et al. "Evaluation on the phagocytosis of apoptotic spermatogenic cells by Sertoli cells in vitro through detecting lipid droplet formation by Oil Red O staining." Reproduction 132, no. 3 (2006): 485–92. http://dx.doi.org/10.1530/rep.1.01213.

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During spermatogenesis, more than half of the differentiating spermatogenic cells undergo apoptosis before they mature into spermatozoa. Ultrastructure studies showed that the formation of lipid droplets in Sertoli cells was associated with phagocytosis of residual bodies and apoptotic germ cells by Sertoli cells. Here, a relationship between the phagocytosis of apoptotic spermatogenic cells and lipid droplet formation in Sertoli cells was studiedin vitroby Oil Red O (ORO) staining. The results confirmed that the formation of lipid droplets was a result of phagocytosis of apoptotic spermatogen

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Muir, Tera, Ingrid Sadler-Riggleman, and Michael K. Skinner. "Role of the Basic Helix-Loop-Helix Transcription Factor, Scleraxis, in the Regulation of Sertoli Cell Function and Differentiation." Molecular Endocrinology 19, no. 8 (2005): 2164–74. http://dx.doi.org/10.1210/me.2004-0473.

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Abstract Sertoli cells are a postmitotic terminally differentiated cell population in the adult testis that form the seminiferous tubules and provide the microenvironment and structural support for developing germ cells. The transcription factors that regulate Sertoli cell differentiation remain to be elucidated. The basic helix-loop-helix transcription factors are involved in the differentiation of a variety of cell lineages during development and are expressed in pubertal Sertoli cells. A yeast-two-hybrid procedure was used to screen a Sertoli cell library from 20-d-old pubertal rats to iden

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Maboundou, Jean-Claude, Mohamed Fofana, Jacqueline Fresnel, Jean Bocquet, and Dominique Le Goff. "Effect of lipoproteins on cholesterol synthesis in rat Sertoli cells." Biochemistry and Cell Biology 73, no. 1-2 (1995): 67–72. http://dx.doi.org/10.1139/o95-008.

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Lipoprotein metabolism has been investigated in cultured rat Sertoli cells. Cells incubated with low-density lipoproteins (LDLs) or high-density lipoproteins (HDLs) showed a concentration-dependent decrease of sterol synthesis, indicating a net cholesterol delivery to the Sertoli cells. At 50 μg/mL, lipoproteins inhibited the incorporation of [14C]acetate into free cholesterol by 83% for the LDL and 47% for the HDL. Electron microscopic examinations of the Sertoli cells provide evidence of the internalization of gold-labelled HDL into coated pits and coated vesicles. Competitive studies betwee

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O’Donnell, Liza, Laura F. Dagley, Michael Curley, et al. "Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid." PLOS ONE 18, no. 9 (2023): e0290846. http://dx.doi.org/10.1371/journal.pone.0290846.

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Sertoli cells support the development of sperm and the function of various somatic cells in the interstitium between the tubules. Sertoli cells regulate the function of the testicular vasculature and the development and function of the Leydig cells that produce testosterone for fertility and virility. However, the Sertoli cell-derived factors that regulate these cells are largely unknown. To define potential mechanisms by which Sertoli cells could support testicular somatic cell function, we aimed to identify Sertoli cell-enriched proteins in the testicular interstitial fluid (TIF) between the

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Chang, Wen-Lin, Lina Cui, Yanli Gu, et al. "TBC1D20 deficiency induces Sertoli cell apoptosis by triggering irreversible endoplasmic reticulum stress in mice." Molecular Human Reproduction 25, no. 12 (2019): 773–86. http://dx.doi.org/10.1093/molehr/gaz057.

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Abstract Male ‘blind sterile’ mice with the causative TBC1 domain family member 20 (TBC1D20) deficiency are infertile with excessive germ cell apoptosis and spermatogenesis arrest at the spermatid stage. Sertoli cells are characterised as ‘nurse cells’ essential for normal spermatogenesis, but the role and corresponding molecular mechanisms of TBC1D20 deficiency in Sertoli cells of mice are not clear to date. In the present study, the histopathology of the testis and Sertoli cell proliferation and apoptosis were determined, and the corresponding molecular mechanisms were investigated by wester

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Ueffing, Mareike, Marion Langeheine, Sarah Gniesmer, et al. "The impact of Connexin 43 deficiency on the cell shape and cytoskeleton of murine Sertoli cells: A house with ramshackle walls?" PLOS One 20, no. 4 (2025): e0321292. https://doi.org/10.1371/journal.pone.0321292.

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Genetically induced loss of the gap-junction protein Connexin 43 (Cx43) in murine Sertoli cells leads to an arrest of spermatogenesis at the level of spermatogonia, highly vacuolated tubules, and intratubular cell clusters. Transmission electron microscopy as well as 3D-reconstruction of Sertoli cells based on serial block-face scanning electron microscopy imaging revealed severe cell shape changes in Cx43 deficient Sertoli cells. Since the cytoskeleton is important for the transport of germ cells within the seminiferous epithelium and for keeping the cell shape, the study at hand aimed to rev

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Kongmanas, Kessiri, Arpornrad Saewu, Wongsakorn Kiattiburut, et al. "Accumulation of Seminolipid in Sertoli Cells Is Associated with Increased Levels of Reactive Oxygen Species and Male Subfertility: Studies in Aging Arsa Null Male Mice." Antioxidants 10, no. 6 (2021): 912. http://dx.doi.org/10.3390/antiox10060912.

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Seminolipid (also known as sulfogalactosylglycerolipid-SGG), present selectively in male germ cells, plays important roles in spermatogenesis and sperm–egg interaction. The proper degradation of SGG in apoptotic germ cells is also as important. Sertoli cells first phagocytose apoptotic germ cells, then Sertoli lysosomal arylsulfatase A (ARSA) desulfates SGG, the first step of SGG degradation. We have reported that aging male Arsa−/− mice become subfertile with SGG accumulation in Sertoli cell lysosomes, typical of a lysosomal storage disorder (LSD). Since reactive oxygen species (ROS) levels a

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Enders, G. C., and C. F. Millette. "Pachytene spermatocyte and round spermatid binding to Sertoli cells in vitro." Journal of Cell Science 90, no. 1 (1988): 105–14. http://dx.doi.org/10.1242/jcs.90.1.105.

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Spermatogenic cells differentiate in vivo while in continuous contact with the Sertoli cell. During differentiation, the spermatogenic cells and Sertoli cells form a number of morphologically distinct stage-specific adhesions. We describe an in vitro assay system for studying the adhesion of spermatogenic cells to Sertoli cell monolayers. Mixed populations of spermatogenic cells or enriched fractions of pachytene spermatocytes and round spermatids were labelled with the vital dye, fluorescein diacetate, prior to their addition to Sertoli cell monolayers so that the adhesion of viable spermatog

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Benbrahim-Tallaa, Lamia, Bénazir Siddeek, Aline Bozec, et al. "Alterations of Sertoli cell activity in the long-term testicular germ cell death process induced by fetal androgen disruption." Journal of Endocrinology 196, no. 1 (2007): 21–31. http://dx.doi.org/10.1677/joe-07-0062.

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Fetal androgen disruption, induced by the administration of anti-androgen flutamide (0.4, 2, and 10 mg/kg day) causes a long-term apoptosis in testicular germ cells in adult male rat offspring. One of the questions raised by this observation is the role of the Sertoli cells in the adult germ cell apoptotic process. It is shown here that Sertoli cells originating from 15-day-old rats treated in utero with the anti-androgen (10 mg/kg d) did no longer protect adult germ cells against apoptosis. Indeed, untreated spermatocytes or spermatids exhibited increased (P<0.0001) active caspase-3 levels

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Chung, Nancy P. Y., and C. Yan Cheng. "Is Cadmium Chloride-Induced Inter-Sertoli Tight Junction Permeability Barrier Disruption a Suitable in Vitro Model to Study the Events of Junction Disassembly during Spermatogenesis in the Rat Testis?*." Endocrinology 142, no. 5 (2001): 1878–88. http://dx.doi.org/10.1210/endo.142.5.8145.

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Abstract The events of germ cell movement during spermatogenesis are composed of intermittent phases of junction disassembly and reassembly. Although primary Sertoli cells cultured in vitro can be used to study junction reassembly, an in vitro model to study the events of junction disassembly is still lacking. We have assessed whether the CdCl2-induced inter-Sertoli tight junction (TJ) permeability barrier disruption in vitro can fill this gap. When Sertoli cells (1.2 × 106 cells/cm2) were cultured on Matrigel-coated bicameral units to allow the assembly of inter-Sertoli TJs, it was manifested

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MRUK, DOLORES, LI‐JI ZHU, BRUNO SILVESTRINI, WILL M. LEE, and C. YAN CHENG. "Interactions of Proteases and Protease Inhibitors in Sertoli‐Germ Cell Cocultures Preceding the Formation of Specialized Sertoli‐Germ Cell Junctions In Vitro." Journal of Andrology 18, no. 6 (1997): 612–22. http://dx.doi.org/10.1002/j.1939-4640.1997.tb02438.x.

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ABSTRACT: The biochemical mechanism(s) by which germ cells can form specialized junctions with Sertoli cells in the seminiferous epithelium at various stages of the spermatogenic cycle is unknown. This study sought to examine the biochemical changes that are involved when germ cells are cocultured with Sertoli cells in vitro preceding the establishment of specialized Sertoli‐germ cell junctions. While isolated germ cells were allowed to attach to Sertoli cells, media from both the apical and basal compartments of bicameral units were collected to assess serine and cysteine protease activity. T

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Chaudhary, Jaideep, Andrea S. Cupp, and Michael K. Skinner. "Role of Basic-Helix-Loop-Helix Transcription Factors in Sertoli Cell Differentiation: Identification of an E-Box Response Element in the Transferrin Promoter*." Endocrinology 138, no. 2 (1997): 667–75. http://dx.doi.org/10.1210/endo.138.2.4942.

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Abstract Sertoli cells are critical for testicular function and maintenance of the spermatogenic process. The induction of Sertoli cell differentiation in the embryo promotes testicular development and male sex determination. The progression of Sertoli cell differentiation during puberty promotes the onset of spermatogenesis. The maintenance of optimal Sertoli cell differentiation in the adult is required for spermatogenesis to proceed. The current study was designed to investigate the transcriptional regulation of Sertoli cell differentiation through the analysis of a previously identified ma

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Othberg, Agneta I., Alison E. Willing, Don F. Cameron, et al. "Trophic Effect of Porcine Sertoli Cells on Rat and Human Ventral Mesencephalic Cells and Hnt Neurons in Vitro." Cell Transplantation 7, no. 2 (1998): 157–64. http://dx.doi.org/10.1177/096368979800700210.

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The poor survival of embryonic dopaminergic (DA) neurons transplanted into patients with Parkinson's disease (PD) has encouraged researchers to search for new methods to affect the short- as well as long-term survival of these neurons after transplantation. In several previous rodent studies Sertoli cells increased survival of islet cells and chromaffin cells when cotransplanted in vivo. The aims of this study were to investigate whether porcine Sertoli cells had a positive effect on the survival and maturation of rat and human DA neurons, and whether the Sertoli cells had an effect on differe

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Cameron, D. F., and K. E. Muffly. "Hormonal regulation of spermatid binding." Journal of Cell Science 100, no. 3 (1991): 623–33. http://dx.doi.org/10.1242/jcs.100.3.623.

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A Sertoli-spermatid coculture model is described in which a large percentage (greater than 76%) of round spermatids remain viable for 48 h and bind to Sertoli cells. The effects of follicle-stimulating hormone (FSH) and testosterone on spermatid binding (expressed as the spermatid density; SD = the number of spermatids per unit area of Sertoli cell cytoplasm), ultrastructure of the Sertoli-spermatid junctional complex, and distribution in the Sertoli cell of junction-related F-actin and vinculin are described. Following 48 h of incubation, neither FSH alone nor testosterone alone affected sper

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Guo, Jian, Shi-Xin Tao, Min Chen, et al. "Heat Treatment Induces Liver Receptor Homolog-1 Expression in Monkey and Rat Sertoli Cells." Endocrinology 148, no. 3 (2007): 1255–65. http://dx.doi.org/10.1210/en.2006-1004.

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We demonstrated in this study that liver receptor homolog-1 (LRH-1) was expressed in the round spermatids in normal monkey testis, and no LRH-1 signal was observed in the Sertoli cells. After local warming (43 C) the monkey testis, however, LRH-1 expression was induced in the Sertoli cells in coincidence with activation of cytokeratin 18 (CK-18), a Sertoli cell dedifferentiated marker. Furthermore, we isolated rat primary Sertoli cells from testes at various stages of development and treated with 43 C water in vitro. The changes in LRH-1 as well as CK-18 expression were analyzed by confocal im

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Fofana, Mohamed, Jean-Claude Maboundou, Jean Bocquet, and Dominique Le Goff. "Transfer of cholesterol between high density lipoproteins and cultured rat Sertoli cells." Biochemistry and Cell Biology 74, no. 5 (1996): 681–86. http://dx.doi.org/10.1139/o96-074.

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In the testes, the Sertoli cells are separated from the blood capillaries by the basement membrane, thereby excluding the passage of low density lipoproteins (LDLs) but allowing the passage of high density lipoproteins (HDLs). The present study examines first the capacity of Sertoli cells to uptake cholesterol from HDL and secondly the role of apolipoproteins (apo) A-I and E in cholesterol flux between HDL and cultured rat Sertoli cells. In the presence of HDL in cultured medium, rat Sertoli cells accumulated few amounts of esterified cholesterol. Incubation of [14C]cholesterol–labelled Sertol

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NEWTON, SEAN C., and CLARKE F. MILLETTE. "Sertoli Cell Plasma Membrane Polypeptides Involved in Spermatogenic Cell‐Sertoli Cell Adhesion." Journal of Andrology 13, no. 2 (1992): 160–71. http://dx.doi.org/10.1002/j.1939-4640.1992.tb01651.x.

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ABSTRACT: This study concerns Sertoli cell‐spermatogenic cell adhesive interactions in the seminiferous tubule. Sertoli cell surface polypeptides involved in germ cell‐Sertoli cell adhesion were identified by serological inhibition of an in vitro Sertoli‐germ cell adhesion assay. This assay was modified from a previously reported adhesion assay, and employs a scanning laser cytometer for quantification of adherent cells. Reactivity of the polyclonal antiserum raised against rat Sertoli cells was also assessed via immunofluorescent microscopy. The addition of antiserum to the adhesion assay res

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Li, Shu Feng, Hui Li Tong, Cong Cong Liu, and Yun Qin Yan. "Effects of IGF-I on the Proliferation of Testis Sertoli Cells in Mouse." Advanced Materials Research 287-290 (July 2011): 112–15. http://dx.doi.org/10.4028/www.scientific.net/amr.287-290.112.

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Sertoli cells are an important part of the male reproductive system. Except the function of promoting spermatogenesis such as supporting, providing nutrition and secreting, it also can maintain the immune privilege of testis through the expression of a special surface molecule named FasL. This feature aviods the immune response triggered by self antigen (germ cells) contacting with the body, which can be used to provide immune privilege environment for transplanted cells in areas other than the testis. In this article, we purified mouse sertoli cells, through adding different concentrations of

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Zhang, Zhen, Shan Shao, Gunapala Shetty, and Marvin L. Meistrich. "Donor Sertoli cells transplanted into irradiated rat testes stimulate partial recovery of endogenous spermatogenesis." REPRODUCTION 137, no. 3 (2009): 497–508. http://dx.doi.org/10.1530/rep-08-0120.

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Irradiation of rat testes leads to the failure to support differentiation of the surviving spermatogonia due to damage of the somatic environment. To determine the involvement of Sertoli cells in this somatic damage, we transplanted seminiferous tubule cells from normal immature GFP-transgenic rats into the testes of irradiated rats. The donor Sertoli cells colonized and developed in the host testes. In many seminiferous tubules, the donor Sertoli cells formed abnormal spherical structures in the lumen, but in some tubules they formed a normal-appearing epithelium, but with only isolated sperm

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RAYCHOUDHURY, SAMIR S., ALAN W. BLACKSHAW, and MICHAEL G. IRVING. "Hormonal Modulation of the Interactions of Cultured Rat Testicular Sertoli and Peritubular Myoid Cells." Journal of Andrology 14, no. 1 (1993): 9–16. http://dx.doi.org/10.1002/j.1939-4640.1993.tb00362.x.

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ABSTRACT: Previous investigators have suggested metabolic cooperation between Sertoli and peritubular cells. This study concerns Sertoli cell and peritubular myoid cell interactions in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). We have tested the effect of hormones and other regulatory agents such as a combination of FSH, insulin, retinol, and testosterone (FIRT) on monocultures of Sertoli and peritubular myoid cells, and have examined whether or not coculture of Sertoli and peritubular myoid cells substitutes for the sti

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Benahmed, M., J. Reventos, E. Tabone, and J. M. Saez. "Cultured Sertoli cell-mediated FSH stimulatory effect on Leydig cell steroidogenesis." American Journal of Physiology-Endocrinology and Metabolism 248, no. 2 (1985): E176—E181. http://dx.doi.org/10.1152/ajpendo.1985.248.2.e176.

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To determine the precise role of Sertoli cells in the stimulating effects of follicle stimulating hormone (FSH) on Leydig cell activity, porcine purified Leydig and Sertoli cells were cultured separately or together in a chemically defined medium in the absence or presence of porcine, FSH 50 ng/ml. Leydig cell activity was evaluated using two parameters: human chorionic gonadotropin (hCG) binding sites; and hCG-stimulated cAMP production and testosterone secretion. First, it was found that FSH increases Leydig cell activity in crude Leydig cell preparations (40–60% of Leydig cells), whereas it

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Ueda, H., L. L. Tres, and A. L. Kierszenbaum. "Culture patterns and sorting of rat Sertoli cell secretory proteins." Journal of Cell Science 89, no. 2 (1988): 175–88. http://dx.doi.org/10.1242/jcs.89.2.175.

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A cocultivation chamber and two types of permeable substrates have been used to study: (1) the culture patterns of rat Sertoli and peritubular cells, and Sertoli cells cocultured with spermatogenic cells or peritubular cells; and (2) the polarized secretion of Sertoli cell-specific proteins transferrin, S70 and S45-S35 heterodimeric protein. Substrates included a nylon mesh (with openings of 100 micron) coated with extracellular matrix (ECM) material and an uncoated microporous filter (with pores of 0.45 micron). Sertoli cells cultured on ECM-coated nylon mesh organized a continuous sheet of m

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Regadera, Javier, Francisco MartÍnez-GarcÍa, Pilar González-Peramato, Alvaro Serrano, Manuel Nistal, and Carlos Suárez-Quian. "Androgen Receptor Expression in Sertoli Cells as a Function of Seminiferous Tubule Maturation in the Human Cryptorchid Testis1." Journal of Clinical Endocrinology & Metabolism 86, no. 1 (2001): 413–21. http://dx.doi.org/10.1210/jcem.86.1.7109.

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Androgen receptor (AR) immunohistochemistry was performed in an archival collection of adult human cryptorchid testes to determine whether AR cellular distribution and intensity of immunostaining were functions of the severity of cellular dysgenesis. The seminiferous tubule histology of cryptorchid testes collected from adults is marked by three specific patterns. 1) Seminiferous tubules are characterized as maintaining focal areas of germinal cell differentiation (albeit incomplete) that are interspersed with 2) tubules composed of Sertoli cells only, these latter cells being principally of t

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Meachem, S. J. "002.Sertoli cell terminal differentiation: doing a 'U' turn on a one way street." Reproduction, Fertility and Development 17, no. 9 (2005): 62. http://dx.doi.org/10.1071/srb05abs002.

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The concept of terminal differentiation of Sertoli cells has been challenged and this new information has important implications for male fertility. The mammalian Sertoli cell has two distinct functions: (i) formation of the seminiferous cords and (ii) provision of nutritional and structural support to the developing germ cells. For these to occur successfully, Sertoli cells must undergo numerous maturational changes between foetal and adult life, the main switches occur around the onset of puberty, coincident with the rise in serum gonadotrophins. These switches include the loss of proliferat

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Nicholls, Peter K., Peter G. Stanton, Justin L. Chen, et al. "Activin Signaling Regulates Sertoli Cell Differentiation and Function." Endocrinology 153, no. 12 (2012): 6065–77. http://dx.doi.org/10.1210/en.2012-1821.

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Abstract Throughout development, activin A signaling stimulates proliferation and inhibits differentiation of testicular Sertoli cells. A decline in activin levels at puberty corresponds with the differentiation of Sertoli cells that is required to sustain spermatogenesis. In this study, we consider whether terminally differentiated Sertoli cells can revert to a functionally immature phenotype in response to activin A. To increase systemic activin levels, the right tibialis anterior muscle of 7-wk-old C57BL/6J mice was transduced with an adeno-associated virus (rAAV6) expressing activin A. We

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LI, JONATHAN C. H., WILL M. LEE, DOLORES MRUK, and C. YAN CHENG. "Regulation of Sertoli Cell Myotubularin (rMTM) Expression by Germ Cells In Vitro." Journal of Andrology 22, no. 2 (2001): 266–77. http://dx.doi.org/10.1002/j.1939-4640.2001.tb02180.x.

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ABSTRACT: Recent studies have shown that rat myotubularin (rMTM), the homolog of human myotubularin, which is a putative protein tyrosine phosphatase (PTP), is expressed by Sertoli cells in the rat testis. In addition, a significant increase in its steady‐state mRNA level was detected in Sertoli cells at the time of inter‐Sertoli tight junction (TJ) assembly in vitro. Since the interplay of protein kinases and phosphatases that determines the intracellular phosphoprotein content can, in turn, regulate the assembly and maintenance of TJ and anchoring junctions (AJ) in vitro, as demonstrated in

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LAMPA, JOHN, JOS W. HOOGERBRUGGE, WILLY M. BAARENDS, et al. "Follicle‐Stimulating Hormone and Testosterone Stimulation of Immature and Mature Sertoli Cells in vitro: Inhibin and N‐cadherin Levels and Round Spermatid Binding." Journal of Andrology 20, no. 3 (1999): 399–406. http://dx.doi.org/10.1002/j.1939-4640.1999.tb02534.x.

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ABSTRACT: The in vitro response of Sertoli cells isolated from adult rat testes to testosterone (T) and follicle‐stimulating hormone (FSH) treatment was investigated. Sertoli cells from >70‐day‐old Sprague‐Dawley rats were isolated by a combined enzymatic treatment followed by the removal of the majority of contaminating germ cells with immobilized peanut agglutinin lectin. Sertoli cells were then cultured for 6–10 days, forming a confluent layer with a cell viability of >83% and 74–77% purity. The contaminating cells were peritubular cells (4–6%), pachytene spermatocytes (4–5%), round s

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Patnaik, A. K., and F. K. Mostofi. "A Clinicopathologic, Histologic, and Immunohistochemical Study of Mixed Germ Cell-Stromal Tumors of the Testis in 16 Dogs." Veterinary Pathology 30, no. 3 (1993): 287–95. http://dx.doi.org/10.1177/030098589303000310.

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In a review of 262 neoplasms in 225 dogs, 18 tumors in 16 dogs were mixed germ cell–stromal tumors containing intimately admixed germ cells and Sertoli cells in tubular structures of various sizes. Seven of the 18 neoplasms were predominantly composed of germ cells, and 11 were predominantly composed of Sertoli cells. In 15 neoplasms, the germ cells were more anaplastic than the Sertoli cells, and in three neoplasms, both cell types were anaplastic. Five of the seven mixed-cell tumors with predominantly germ cells had germ cells infiltrating the adjoining tissues. Immunocytochemical studies of

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Sawaied, Alaa, Bat-El Levy, Eden Arazi, Eitan Lunenfeld, Qinghua Shi, and Mahmoud Huleihel. "Follicle-Stimulating Hormone and Testosterone Play a Role in the Regulation of Sertoli Cell Functions Following Germ Cell Depletion In Vitro." International Journal of Molecular Sciences 26, no. 6 (2025): 2702. https://doi.org/10.3390/ijms26062702.

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Spermatogenesis is a process of self-renewal of spermatogonial stem cells and their proliferation and differentiation to generate mature sperm. This process involves interactions between testicular somatic (mainly Sertoli cells) and spermatogonial cells at their different stages of development. The functionality of Sertoli cells is regulated by hormones and testicular autocrine/paracrine factors. In this study, we investigated the effects of follicle-stimulating hormone (FSH) and testosterone addition on Sertoli cell cultures that undergo hypotonic shock, with a primary focus on Sertoli cell a

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Enders, G. C., J. H. Henson, and C. F. Millette. "Sertoli cell binding to isolated testicular basement membrane." Journal of Cell Biology 103, no. 3 (1986): 1109–19. http://dx.doi.org/10.1083/jcb.103.3.1109.

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We have examined the adhesion of primary Sertoli cells to a seminiferous tubule basement membrane (STBM) preparation in vitro. The STBM isolation procedure (Watanabe, T.K., L.J. Hansen, N.K. Reddy, Y.S. Kanwar, and J.K. Reddy, 1984, Cancer Res., 44:5361-5368) yields segments of STBM that retain their histotypic form in both three-dimensional tubular geometry and ultrastructural appearance. The STBM sleeves contain two laminae: a thick, inner basal lamina that was formed in vivo between Sertoli cells and peritubular myoid cells; and a thinner, outer basal lamina that was formed between myoid ce

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DUFOUR, JANNETTE M., RAY V. RAJOTTE, and GREGORY S. KORBUTT. "Development of an In Vivo Model to Study Testicular Morphogenesis." Journal of Andrology 23, no. 5 (2002): 635–44. http://dx.doi.org/10.1002/j.1939-4640.2002.tb02305.x.

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ABSTRACTWe have developed an in vivo model to examine testicular cord formation by isolated Sertoli and myoid cells when implanted under the kidney capsule of severe combined immunodeficient (SCID) mice. Neonatal porcine Sertoli (92.5% ± 3.5%) and myoid (2.2% ± 0.7%) cellular aggregates were transplanted underneath the kidney capsule of SCID mice. Grafts were removed between 0 and 60 days posttransplantation and examined histologically for the progressive development of structures resembling testicular cords. Aggregates began to reorganize by day 3, and cord structures were present at day 7 po

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