Relevant bibliographies by topics / Serum biochemical profile

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Serum biochemical profile'

Author: Grafiati
Published: 4 June 2021
Last updated: 6 February 2022

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Journal articles on the topic "Serum biochemical profile"

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Souza, D. C., D. G. Silva, T. G. Rocha, B. M. Monteiro, G. T. Pereira, L. C. Fiori, R. B. Viana, and J. J. Fagliari. "Serum biochemical profile of neonatal buffalo calves." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 71, no. 1 (February 2019): 187–96. http://dx.doi.org/10.1590/1678-4162-10176.

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ABSTRACT Serum blood samples from 50 Murrah buffalo calves were examined in this study. The animals were allocated into three groups according to the number of parturitions of their mothers: G1 (n= 15) calves from primiparous buffaloes, G2 (n= 19) calves from buffaloes with two to four parturitions, and G3 (n= 16) calves from buffaloes with five or more parturitions. Blood samples were taken at birth, before colostrum ingestion, at 24h, 48h, and 72h after birth, and at 7, 14, 21, and 30 days after birth for determination of levels of gammaglutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatine kinase, total protein, albumin, globulins (including immunoglobulin G), iron, total calcium, ionized calcium, phosphorus, sodium, and potassium. The age of the calves was found to influence all of the biochemical parameters, with the exception of ionized calcium and potassium in the calves in groups G1 and G3. The calving order was found to influence AST, GGT, total protein, albumin, and globulins, including IgG. The high serum ALP activity in the first two days after birth indicates that measurement of the levels of this enzyme may be used as an indirect method of assessing passive immunity transfer.
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Stockham, Steven L. "Interpretation of Equine Serum Biochemical Profile Results." Veterinary Clinics of North America: Equine Practice 11, no. 3 (December 1995): 391–414. http://dx.doi.org/10.1016/s0749-0739(17)30307-3.

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Varanis, Laura Ferrari Monteiro, Karla Alves Oliveira, Carolina Moreira Araújo, Wendell Fernando Guimarães da Cruz, and Gilberto de Lima Macedo Júnior. "Serum biochemical reference ranges for pregnant sheep." Bioscience Journal 37 (July 2, 2021): e37036. http://dx.doi.org/10.14393/bj-v37n0a2021-47695.

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Late pregnancy is accompanied by dramatic changes in the metabolism of sheep, in which the nutrient requirements increase and the metabolic capacity of ewes is under severe stress due to the rapid foetal growth and development of the mammary gland. The blood metabolic profile can be used to monitor these alterations, which can lead to metabolic disorders such as pregnancy toxaemia. However, data available on serum parameters in sheep do not consider physiological state. Therefore, the present study aimed to determine the biochemical reference ranges for pregnant ewes, including serum energy, protein and enzyme-related metabolites. Data from a variety of metabolites were obtained from experiments performed in several institutions and commercial farms using Santa Inês, Dorper, Lacaune, Morada Nova, Bergamacia and Suffolk ewes reared under different conditions (grazing, feedlot, semi-feedlot, collective and/or individual pens, and metabolic cages) from 2006 to 2017. All animals were healthy and without feed restriction. Data from ewes with any clinical manifestations were removed. The metabolic energy profile included data of glucose, cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein and very-low-density lipoprotein levels; the metabolic protein profile included the metabolites total protein, uric acid, urea, albumin, and creatinine; and the metabolic enzymatic profile included the enzymes aspartate aminotransferase, gamma-glutamyl transferase and alkaline phosphatase. The reference ranges were estimated using the software RefVal 4.11. Dixon’s test was used to identify and remove outliers. The confidence intervals and percentiles were estimated using the nonparametric method of bootstrap when data were not normally distributed. A 95% confidence level was used. The serum biochemical reference ranges for pregnant sheep determined in our study were strongly divergent from those established by one of the most cited books on the topic, especially considering the high serum urea and cholesterol concentrations and low levels of blood glucose observed. Therefore, it is essential to consider physiological status when evaluating the blood metabolic profile of pregnant ewes in order to maintain an adequate nutritional management and to prevent health disorders that may lead to productive and reproductive losses.
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de Miranda, Renata Lima, Antonio Vicente Mundim, Ana Carolina Silveira Saquy, Álisson Souza Costa, Ednaldo Carvalho Guimarães, Felipe César Gonçalves, and Frederico Ozanam Carneiro e Silva. "Biochemical serum profile of equines subjected to team penning." Comparative Clinical Pathology 18, no. 3 (January 10, 2009): 313–19. http://dx.doi.org/10.1007/s00580-008-0803-6.

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Veena, M. P., K. Nagarajan, S. P. Vairamuthu, S. Subapriya, H. Dhanalaksmi, G. P. Kalmath, H. S. Shwetha, and K. Megaha. "Evaluation of Serum Biochemical Profile of Kidney Disorders in Canine." International Journal of Current Microbiology and Applied Sciences 9, no. 3 (March 10, 2020): 700–705. http://dx.doi.org/10.20546/ijcmas.2020.903.084.

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Ramesh, D., V. Aswathanarayanappa, G. Kumar, M. Vinuthan, M. Veena, and M. Shivakumar. "Study on Serum Biochemical Profile in Hassan Sheep of Karnataka." International Journal of Livestock Research 8, no. 5 (2018): 98. http://dx.doi.org/10.5455/ijlr.20170411093512.

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Almeida, Adriana Jardim de, Letícia da Silva Leite, Luiz Antônio Eckhardt, Antônio Peixoto Albernaz, Anderson Barros Teixeira, and Karoll Andrea Alfonso Torres. "Serum biochemical profile of emus (Dromaius novaehollandiae) reared in captivity." Journal of Applied Animal Research 46, no. 1 (August 24, 2017): 593–98. http://dx.doi.org/10.1080/09712119.2017.1367686.

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Bayir, Abdulkadir, A. Necdet Sirkecioğlu, Harun Polat, and N. Mevlüt Aras. "Biochemical profile of blood serum of siraz Capoeta capoeta umbla." Comparative Clinical Pathology 16, no. 2 (March 13, 2007): 119–26. http://dx.doi.org/10.1007/s00580-006-0663-x.

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Acharya, R., and P. B. Pal. "Study of Hematological and Serum Biochemical Profile in Preparturient Murrah Cross Buffaloes in Chitwan District." Nepalese Veterinary Journal 34 (December 21, 2017): 36–40. http://dx.doi.org/10.3126/nvj.v34i0.22892.

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The aim of this study was to establish the reference values for hematology and serum biochemistry of pre-parturient Murrah crossbred buffaloes of Western Chitwan (Bharatpur Metropolitancity). The study was cross-sectional in which31 apparently healthy pregnant Murrah cross buffaloes ranging from 5-8 years were selected randomly after confirmation by rectal palpation. Blood samples collected from jugular vein were stored in EDTA tubes for hematological analysis and for biochemical analysis centrifuged at 1500 rpm for 20 minutes to separate serum after 1 hour of collection. Hematological indices were determined manually using routine laboratory techniques and serum biochemicals were analysed using automated analyzer (Dimension RXL). Serum biochemicals like Glucose, BUN, Creatinine, Calcium, Phosphorous, Magnesium, AST, ALT, total serum Protein and albumin level were found within the normal range. Similarly, hematologic indices Hb, PCV, Platelet counts, RBC counts, WBC counts, and differential leukocyte counts were also in normal ranges. However, eosinophil (7.83±0.82), and monocyte (4.4±0.53) counts were higher at significant numbers which suggests gastrointestinal parasitic infestations and/or allergic diseases along with some chronic infectious diseases in the buffalo herd of Bharatpur Metropolitancity in western Chitwan.
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Wardoyo, Suwaji Handaru, and Solichan Badri. "Effect Acupuncture for Low Back Pain; Biochemical and Protein Profile Analysis." Jurnal Kesehatan Masyarakat 16, no. 2 (November 29, 2020): 207–15. http://dx.doi.org/10.15294/kemas.v16i2.22650.

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Low back pain (LBP) is one of the common symptoms experienced by some of the world community. Acupuncture as a method of healing a disease offers a solution for LBP symptom. This study aimed to determine the effect of acupuncture with local and Yamamoto New Scalp Acupuncture (YNSA) points with biochemical analysis related to total protein, albumin, and globulin, and analysis of blood serum protein profiles using the SDS-PAGE method. This research was conducted using sterile acupuncture needles that inserted in patients with several local points, namely BL22 (Sanjiaoshu), BL23 (Shenshu), BL24 (Qihaishu), BL25C (Dachangshu), and BL26C (Guanyuanshu) and combined with the YNSA points through somatotope D points and extra lumbar points H and I. Blood serum was collected for total protein, albumin, and globulin analysis. That blood serum was also used for protein profile analysis using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. The results showed that six times of acupuncture treatments gave positive correlation in total protein, albumin, and globulin. Analysis of blood serum protein profiles using SDS-PAGE showed a protein band of about 12 KDa and it might be a interleukin-13 protein. The results were expected can be useful as scientific information especially related to acupuncture for LBP treatment and this of about 12 KDa protein band can be used as biomarker candidates for the symptoms.
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Dissertations / Theses on the topic "Serum biochemical profile"

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Traesel, Carolina Kist. "Perfil bioquímico sérico de frangos de corte alimentados com dieta suplementada com óleos essenciais e pimenta." Universidade Federal de Santa Maria, 2009. http://repositorio.ufsm.br/handle/1/10067.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico
Essential oils of natural origin and low cost have antimicrobial properties and can increase broilers performance, constituting an alternative to growth promoters based on antibiotics for poultry production; however, they can produce toxic effects. Serum biochemical parameters regarding to pancreatic, renal and hepatic functions of broiler chickens fed diets supplemented with antibiotics or essential oils of oregano, sage, rosemary and pepper aqueous crude extract (OLES) were evaluated. Animals (n=910) were distributed within five treatment groups with seven replicates of 26 birds each. Control group received a diet without additives; treatment Tatb received an antibiotic growth promoter diet; treatments T50, T100 and T150 received feed supplemented with 50, 100 and 150 ppm of OLES, respectively. At 42 days, 11 animals per group were slaughtered and blood samples were collected for serum biochemical profile analysis (lipase, amylase, urea, uric acid, aspartate aminotransferase - AST, gama glutamyltransferase, total cholesterol, high-density lipoprotein, low-density lipoprotein, triglycerides, total protein, albumin, globulins and albumin:globulins ratio). The results showed elevated serum lipase, uric acid, urea and AST, suggesting the impairment of kidney and liver functions caused by OLES. More studies are needed for essential oils dose adjustment in the diet, providing benefits without being harmful to the animal.
Devido às exigências atuais do mercado externo por alimentos de qualidade, custo acessível e de origem natural, os extratos de plantas com atividade antimicrobiana, como os óleos essenciais, são uma alternativa aos promotores de crescimento à base de antibióticos na produção de frangos, podendo aumentar o desempenho dos animais. Essas substâncias possuem rápida metabolização hepática, curta meia vida e eliminação renal e podem produzir efeitos tóxicos em altas doses ou estimular a secreção pancreática. Nesse contexto, avaliaram-se as funções pancreática, renal e hepática de frangos de corte alimentados com ração isonutritiva, antibióticos ou óleos essenciais de orégano, sálvia, alecrim e extrato aquoso de pimenta (OLES) em diferentes concentrações na ração. Novecentos e dez frangos foram alocados em cinco grupos de tratamento, com sete repetições de 26 aves por tratamento. O grupo controle recebeu ração sem aditivos; o tratamento Tatb recebeu ração suplementada com promotor de crescimento à base de antibióticos; os tratamentos T50, T100 e T150 receberam ração suplementada com OLES nas concentrações de 50, 100 e 150 ppm, respectivamente. Aos 42 dias de idade, 11 aves por grupo foram abatidas e amostras de sangue foram colhidas para realização das mensurações séricas de lipase, amilase, uréia, ácido úrico, aspartato aminotransferase (AST), gama glutamiltransferase, colesterol total, lipoproteína de alta densidade, lipoproteína de baixa densidade, triglicerídeos, proteínas totais, albumina, globulinas e relação albumina:globulinas. Os resultados obtidos demonstraram elevação sérica significativa de lipase, ácido úrico, uréia e AST, sugerindo o comprometimento renal e hepático pelos OLES. Mais estudos são necessários para adequar as doses dos óleos essenciais na ração, que propiciem benefícios sem provocar danos ao animal.
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Fu-Chien, Lai, and 賴富健. "The Serum Biochemical Profile of Formosan puppies." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/79848972932064255460.

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碩士
國立中興大學
獸醫學系
87
Serum biochemistry is used as an important indicator not only for disease diagnosis, but also for physiological and pharmacological examinations in animals. At present, the principle biochemical values of canines are based on adult samples. However, biochemical variations in puppies during their growing process is seldem reported. Eleven newborn Formosan puppies were used as the experimental group and six adults with an average age of 2.4 years old as the control group. The variations of serum biochemical profile in 2 groups were continuously observed. Collections of puppy serum samples were performed at birth and 24 hours after parturition, and then once a week for 28 weeks. The collection time of blood samples was identical in adults. The serum biochemical components, including alanine aminotransferase( ALT ), alkaline phosphatase( ALP ), aspartate aminotransferse( AST ), blood urea nitrogen( BUN ), calcium( Ca ), creatinine, g-glutamyl transpeptidase( GGT ), glucose, inorganic phosphate( IP ), lactate dehydrogenase( LDH ), total bilirubin( T.B. ), and total protein( TP ) were examined. All samples were analyzed by a Ciba-CorningO 550 Express Plus clinical biochemical analyze, and the analysis of serum protein and alkaline phosphatase isoenzymes were performed by a Helena Rep electrophoretic analyzer. Upon intake of colostrum, all values of the serum biochemical indices, except creatinine, were increased in the neonatal puppies( 1-day old ). In the growing puppies, levels of Ca、CK、IP、bone ALP( BALP ) and unknown ALP( UALP ) gradually decreased while TP, Alb, b2- and g-globulins gradually increased with age. Although the serum biochemical indices of the puppies were different from the adult samples, they were still in the normal range. Striking variations occurred at two weeks postpartum, but gradually stabilized approaching the adult index. By 28 weeks, the puppy indices appeared no different from that of adults, except ALP, Ca, CK, creatinine, IP, TP, a1-, b1-, b2-, g-globulin, liver ALP( LALP ), BALP and UALP. These results can clearly demonstrate that adult canines are much more ideal than puppies to serve as experimental subjects for physiological examinations.
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Hsei, Rac, and 謝志昇. "The relationship of dairy cows' serum biochemical profile and anagement, season and reproductive performance in Taiwan." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/97342556714416125300.

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碩士
國立中興大學
獸醫學系
82
For realizing the relationship of diary cows' serum biochemical profile with management, season, and reproductive performance in Taiwan, 414 blood samples were collected from 4 different farms. The samples were grouped by the farms, pregnancy, seasons and the health condition of the cows. After analyzed the serum samples with Hitachi 7050 automatic analyzer, the results were analyzed by GLM procedure in SAS. The results were shown as followed: 1)Pregnant cows had higher magnesium (Mg), triglyceride (Tg) con- cerntration and alkaline phosphatase (ALP) activity. The cowsorn in 40 days had higher total protein (TP) and globumin (Glob) concentration and lower cholesterol (Chol) concentration than the cows open more than 90 days. Normal open cows have higher calcium (Ca), Mg and Chol concentration and ratio of Ca and phosphate (P) and lower total bilirubin (Tbil) concentration than the cows with retained placenta. 2)In seasons, cold one had the highest Tg concentration and ALP activity, hot one had lowest Chol concentration and highest Tbil concentration, and the one with fickle temperatures had highest TP, blood urea nitrogen (BUN) and Ca concentration and ratio of Ca and P, and lowest P and Tg concentration. 3)Expect of Tbil, there were noticable differences in the serum biochemial profile between farms. 4)When the reproductive performance was improved, the serum bio- chemical profile would change. According the results, the different management would affect the serum biochemical profile, and seasonal variations affected the concentration of most serum constituents, and when the reproductive performance was improved, the serum biochemicalrofile would change.
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Shin, Liao Jia, and 廖家信. "The relationships between serum biochemical profile, body condition scores and diseases during eight weeks prepartum and postpartum." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/87541478323352079955.

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碩士
國立中興大學
獸醫學系
83
Serum biochemistry and body condition scores (BCS) at 4 weeks prepartum and 4 weeks postpartum period were studied in 64 primiparous and multiparous Holstein dairy cows. According to their clinical conditions, these cows were divided into 6 groups: i) 11 normal cows; ii) 23 cows with prolonged open days greater than 90 days; iii) 6 cows that showed anestrous; iv) 12 cows with postpartum uterine infection; v) 7 cows with laminitis during pre- and post- partum period; vi) 5 cows tha t were removed from the herds after calving. Five hundred and twelve serum samples were collected from the 64 cows. From the results, changes in serum biochemical profiles and BCS were observed in accordance to the reproductive and disease status of the cows before and after parturition.
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Yueh-shein, Lin, and 林岳賢. "The different of ovary activity, uterine activity and serum biochemical profile during twenty weeks after parturition in dairy cow." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/25670236457377960043.

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碩士
國立中興大學
獸醫學系
82
There were four purposes to study: In order to clarify.1)The alteration of ovary, uterine activity and serum biochemical profile during 20 weeks afterr parturition. 2) The relationship between the convalescenceof ovary, uterine and biochemical confstituent values in serum. 3)The difference which the mean of ovary, uterine activition and biochemical constituent values within eight groups. 4)The effect of ovary, uterine activition and biochemical constituent values whereof day open. The results of every items tested were all within normal range, the statistical analysis reveealed significant differences among some groups, which were presented as follows: 1) The ovary activity, urterine activity and values of AST, ALT, ALP, GGT, BUN, Cholesterol, Total Protein, Albumin, Ca/P, P were change regularly during 20 weeks after parturition. 2) The ovary activity were correlated with ALT, BUN, Cholesterol, and the uterine activity were correlated with AST, ALT,ALP, GGT, BUN, Cholesterol, Total Protein, Albumin, Ca/P, P. 3) There were significantly different within eight cow-groups expect Ca/P, Ca,and Mg. 4) The days open were correlated with ovary activity, uterine, AST, ALT, ALP, GGT, A/G, Albumin, Globulin, and Ca.
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Lin, Zheng-Yi, and 林政毅. "Effects of season, ovarian hormones, uterus status and serum biochemical profile on uterine protein secretion of dairy cow in Taiwan." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/39192133239135026551.

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Books on the topic "Serum biochemical profile"

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Lopez, Berenice, and Patrick J. Twomey. Biochemical investigation of rheumatic diseases. Oxford University Press, 2013. http://dx.doi.org/10.1093/med/9780199642489.003.0062.

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It is important for rheumatologists to have an understanding of biochemical tests including an awareness of their limitations. The biological variability of an analyte both within and between individuals, the limitations of the measurement technology, the sensitivity of laboratory internal quality control and external quality assurance procedures, as well as interlaboratory variations in practices including sample collection procedures, may all impact on the interpretation of a result. Biochemical tests are often requested to monitor organ-specific dysfunction arising as an adverse consequence of pharmacotherapy or as a component of a systemic rheumatic disease, although dysfunction may also reflect infection or coincidental pathology. Patients with rheumatic diseases are at high risk of renal and hepatic disease. Serum creatinine and its derivative estimated glomerular filtration rate (eGFR) are the most readily available surrogate markers of GFR and are used to assess renal impairment and monitor its course. However, the use of creatinine alone lacks sensitivity and a substantial loss of function must occur before creatinine levels are increased. Additional biochemical screening for kidney damage can be performed by assessment of glomerular integrity, including proteinuria or albuminuria and haematuria. A wide spectrum of rheumatic diseases can affect the liver with various degrees of involvement and hepatic pathology. These often present with cholestatic or hepatitic biochemical profiles. The medical management of rheumatic diseases also involves medications that are hepatotoxic, and routine monitoring of liver function is recommended. This approach is not problem-free and may be improved by quantitative determinations of non-invasive markers of liver fibrosis in the future. Together with imaging techniques, biochemical tests play an important role in the assessment and differential diagnosis of metabolic bone disease.
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Book chapters on the topic "Serum biochemical profile"

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Bityutskyy, Volodimir, Svitlana Tsekhmistrenko, Oksana Tsekhmistrenko, Olexander Melnychenko, and Viktor Kharchyshyn. "Effects of Different Dietary Selenium Sources Including Probiotics Mixture on Growth Performance, Feed Utilization and Serum Biochemical Profile of Quails." In Modern Development Paths of Agricultural Production, 623–32. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-14918-5_61.

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Lopez, Berenice, and Patrick J. Twomey. "Biochemical investigation of rheumatic diseases." In Oxford Textbook of Rheumatology, 451–56. Oxford University Press, 2013. http://dx.doi.org/10.1093/med/9780199642489.003.0062_update_001.

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It is important for rheumatologists to have an understanding of biochemical tests including an awareness of their limitations. The biological variability of an analyte both within and between individuals, the limitations of the measurement technology, the sensitivity of laboratory internal quality control and external quality assurance procedures, as well as interlaboratory variations in practices including sample collection procedures, may all impact on the interpretation of a result. Biochemical tests are often requested to monitor organ-specific dysfunction arising as an adverse consequence of pharmacotherapy or as a component of a systemic rheumatic disease, although dysfunction may also reflect infection or coincidental pathology. Patients with rheumatic diseases are at high risk of renal and hepatic disease. Serum creatinine and its derivative estimated glomerular filtration rate (eGFR) are the most readily available surrogate markers of GFR and are used to assess renal impairment and monitor its course. However, the use of creatinine alone lacks sensitivity and a substantial loss of function must occur before creatinine levels are increased. Additional biochemical screening for kidney damage can be performed by assessment of glomerular integrity, including proteinuria or albuminuria and haematuria. A wide spectrum of rheumatic diseases can affect the liver with various degrees of involvement and hepatic pathology. These often present with cholestatic or hepatitic biochemical profiles. The medical management of rheumatic diseases also involves medications that are hepatotoxic, and routine monitoring of liver function is recommended. This approach is not problem-free and may be improved by quantitative determinations of non-invasive markers of liver fibrosis in the future. Together with imaging techniques, biochemical tests play an important role in the assessment and differential diagnosis of metabolic bone disease.
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Conference papers on the topic "Serum biochemical profile"

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Zhou, B., and J. She. "Serum Metabolite Profiles as Potential Biochemical Markers in Young Patients with Community Acquired Pneumonia." In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a7438.

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Hunter, N. R., I. R. MacGregor, J. Dawes, and D. S. Pepper. "MICROCARRIER CULTURE OF HUMAN ENDOTHELIAL CELL TYPES - A SOURCE OF METABOLITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643348.

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The production of human endothelial cell secretory products in amounts sufficient for biochemical studies is largely restricted by the culture growth area. Conventional flat bed systems yield at best 20-30 x 106 cells per 180cm2 culture flask. To overcome this problem, cells may be grown on Cytodex 3 microcarriers allowing large numbers of cells to be grown and conditioned in small culture volumes. A typical microcarrier unit will contain 200-300 x 106 cells and may be expanded in excess of 1000 x 106 cells at confluence. High viability (95%) and recovery (70-80%) in sub-culturing of microcarrier to microcarrier culture can be achieved with careful management of culture conditions and brief exposure to enzymes.Human umbilical artery and vein, and saphenous vein endothelial cells were prepared and grogn on microcarrier cultures to cell populations of 200-450 x 106 cells and conditioned for 14 day periods in serum-free media.The production profiles of several endothelial cell proteins including thrombospondin (TSP), von Willebrand Factor (vWF) and issue plasminogen activator (t-PA) were measured by radioimmunoassay under these conditions, and demonstrate the use of microcarrier cultures in producing milligram quantities of engothelial cell protein. For example, a HUVEC culture of 200 x 106 cells conditioned with serum-free media for 14 days yielded a total of 6.9mg TSP, 0.7mg vWF and 48.9ug t-PA. In this laboratory one such application of the system was the purification of endothelial proteins in amounts sufficient for immunisation of mice prior to the production of monoclonal antibodies and for subsequent characterisation.
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