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Rahardjani, Kamilah Budhi. "Hubungan antara Malondialdehyde (MDA) dengan Hasil Luaran Sepsis Neonatorum." Sari Pediatri 12, no. 2 (2016): 82. http://dx.doi.org/10.14238/sp12.2.2010.82-7.
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Latar belakang. Sepsis merupakan penyebab morbiditas dan mortalitas terbanyak pada neonatus. Pada sepsis terjadi peningkatan stres oksidatif yang menyebabkan kerusakan jaringan dan hemolisis. Kadar MDA serum merupakan biomarker adanya stress oksidatif.Tujuan. Mengetahui hubungan antara MDA serum dengan hasil luaran sepsis pada neonatus.Metode. Penelitian observasional prospektif dilakukan pada neonatus sepsis yang dirawat di PBRT (Peawatan Bangsal Risiko Tinggi) RSUP Dr. Kariadi Semarang, Oktober 2007 sampai dengan Januari 2008. Diagnosis sepsis berdasar gejala klinik dan pemeriksaan laboratorium. Keluaran sepsis dikelompokkan menjadi dua yaitu perburukan (BR) dan perbaikan (BI). Kadar MDA serum diukur dua kali saat terdiagnosis sepsis (MDA1) dan pada hari ke-5 atau bila terjadi disfungsi organ / meninggal / perburukan (MDA2) dengan menggunakan metode spektofotometri. Analisis statistik menggunakan Uji Wilcoxon Signed – Rank, Mann-Whitney, ROC analysis dan Fisher-exact.Hasil. Subjek penelitian terdiri dari 41 neonatus sepsis, 33 neonatus BI dan 8 neonatus BR. Rerata kadar MDA1 2,97±0,14 dan MDA2 3,05±0,34. Kadar MDA 1 kelompok BI 2,95±0,117, kelompok BR 3,08±0,172 (p=0,03). Kadar MDA2 kelompok BI 2,97±0,182, kelompok BR 3,38±0,591 (p=0,006). Kelompok BI MDA2 meningkat tak bermakna (p=0,9), kelompok BR, MDA2 meningkat bermakna (p=0,01). Kurva ROC luas area bawah kurva MDA1 0,75 (p=0,03), cut-off-point= 2,928 ng/mL. Dijumpai hubungan bermakna antara kategori MDA1 dengan luaran sepsis (p=0,02). Risiko relatif MDA1 ≥2,928 ng/mL untuk hasil luaran sepsis perburukan 7,4 X (95% CI=1,4-37,2).Kesimpulan. Terdapat hubungan antara kadar MDA serum dengan hasil luaran sepsis neonatorum. (
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Crnogaj, M., R. Petlevski, V. Mrljak, et al. "Malondialdehyde levels in serum of dogs infected with Babesia canis." Veterinární Medicína 55, No. 4 (2010): 163–71. http://dx.doi.org/10.17221/77/2010-vetmed.
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Babesiosis is a common tick born disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The aim of the present study was to confirm the presence of oxidative stress by examining serum malondialdehyde (MDA), an end product of lipid peroxidation, in 35 dogs naturally infected with Babesia canis (B. canis). MDA was examined in 14 healthy dogs as well. Blood smears were prepared from peripheral blood and they showed the presence of B. canis in infected dogs. B. canis was confirmed using the PCR (Polymerase chain reaction) method. On the basis of clinical and laboratory data the 35 infected dogs were clinically classified into two groups, complicated (seven dogs) and uncomplicated (28 dogs). The noted complications were renal dysfunction (5/7), hepatic dysfunction (3/7), muscular involvement (2/7) and ARDS (1/7). Levels of blood urea nitrogen concentration (BUN), creatinin, total bilirubin, alanin aminotranspherase (ALT), alkaline phosphatase (AP), and gammaglutamil aminotranspherase (GGT) were significantly increased in dogs with complicated versus uncomplicated babesiosis. Furthermore the uncomplicated group of dogs was, depending on the severity of anaemia, classified as suffering from severe, moderate or mild disease. Levels of serum MDA were significantly higher in sick dogs (36.90 μmol/l ± 13.95) than healthy animals (8.13 μmol/l ± 1.78). There was no significant statistical difference in serum MDA levels between dogs with complicated (38.48 μmol/l ± 12.11) and uncomplicated babesiosis (36.50 μmol/l ± 14.55). Comparison of the groups based on the severity of anaemia showed that there was no significant statistical difference in serum MDA levels between them. The study demonstrated the involvement of oxidative damage in dogs naturally infected with B. canis.
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Widowati, Hesty, Hidayat Sujuti, and Karyono Mintaroem. "Effect of per oral sipermetrin exposure on serum 17 estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)." Majalah Obstetri & Ginekologi 26, no. 1 (2018): 20. http://dx.doi.org/10.20473/mog.v1i12018.20-25.
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Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).
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Widowati, Hesty, Hidayat Sujuti, and Karyono Mintaroem. "Effect of per oral sipermetrin exposure on serum 17-beta estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)." Majalah Obstetri & Ginekologi 26, no. 1 (2018): 20. http://dx.doi.org/10.20473/mog.v26i12018.20-25.
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Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).
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Sagar, Pallavi, Kumar Pranay, Ravi Shekhar, Prit Pal Singh, and Praveen Kumar. "Serum malondialdehyde in different stages of chronic renal disorder." Asian Journal of Medical Sciences 14, no. 6 (2023): 113–18. http://dx.doi.org/10.3126/ajms.v14i6.52003.
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Background: Chronic kidney disease (CKD) is a widespread public health problem, which may have several adverse consequences such as renal failure, cardiovascular disease, and premature death. Kidney-related pathologies have increasing prevalence rates, produce a considerable financial burden, and are characterized by elevated levels of oxidative stress (OS). Several markers emerged as well-suited indicators of OS such as malondialdehyde (MDA) and lipid hydroperoxides. The reduced activities of antioxidant enzymes status and increased production of MDA in the CKD patients confirm the presence of OS. The alteration in antioxidant status and MDA in CKD patients supports the role of OS in CKD patient. Aims and Objectives: The aim of the study was to compare serum MDA in different stages of CKD with that of control. It is well known that inflammation has an important role in CKD and MDA is an oxidant biomarker. Materials and Methods: This was a cross-sectional study having 400 participants with 300 known cases of CKD and 100 healthy controls. Serum MDA levels were measured by thiobarbituric acid assay. Results: There was a significant difference between the groups regarding the MDA values (P<0.001) with that of control. The mean MDA value in Stage III (5.64±1.93 Umol/L), Stage IV (6.14±1.584 Umol/L), and Stage V (10.761±3.347 U moL/L) of CKD patients were high in comparison to healthy control (1.88±0.181 U mol/L). Conclusion: We concluded that MDA is a useful biomarker in CKD patients. The correlations of serum MDA among different stages of CKD patients were significant. Larger studies focused on CKD severity and antioxidant/oxidant biomarkers are required.
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Merendino, Rosaria Alba, Francesco Salvo, Antonella Saija, et al. "Malondialdehyde in benign prostate hypertrophy: a useful marker?" Mediators of Inflammation 12, no. 2 (2003): 127–28. http://dx.doi.org/10.1080/0962935031000097745.
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Benign prostate hypertrophy (BPH) is the most common benign tumor in men due to obstruction of the urethra and, finally, uremia. Malondialdehyde (MDA) is a product derived from peroxidation of polyunsaturated fatty acids and related esters. Evaluation of MDA in serum represents a non-invasive biomarker of oxidative stress. Prostate-specific antigen (PSA) is a sensitive marker for prostatic hypertrophy and cancer. We analyzed MDA serum levels to evaluate the oxidative stress in BPH. To this end, 22 BPH patients and 22 healthy donors were enrolled. Data show an increase of MDA level in BPH patients and a positive correlation between PSA and MDA levels. In conclusion, we describe a previously unknown relationship between PSA and MDA as an index of inflammation and oxidative stress in BPH.
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U., Sreenivasulu, Kishore A.V Rama, R. Shyam Prasad B., Durga T., and Priya Dsouza Hilda. "Study of Serum Sialic Acid and Malondialdehyde Levels in Chronic Renal Failure Patients: A Hospital Based Study in Government General Hospital, Anantapur, Andhrapradesh." International Journal of Toxicological and Pharmacological Research 12, no. 2 (2022): 133–39. https://doi.org/10.5281/zenodo.12533262.
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<strong>Background: </strong>Chronic renal failure (CRF) is a progressive and irreversible destruction of kidney tissue. Renal failure is the cause of death in uncontrolled diabetes especially in the case of early onset insulin dependent diabetes. Serum sialic acid, a marker of acute phase response is related to the presence of diabetic micro and macro vascular complications. Malondialdehyde (MDA) is a highly toxic product formed by lipid peroxidation by free radicals. The concentration of MDA is high in diabetic patients. <strong>Aim: </strong>The aim of the Present study is estimation of serum sialic acid, serum malon dialdehyde (MDA), in Chronic Renal failure patients (CRF) and also to study the correlation between serum sialic acid, serum MDA and plasma glucose and serum creatinine levels. <strong>Materials and Methods: </strong>Present study was done in the Govt General Hospital, Anantapur; Andhra Pradesh. 100 subjects were taken for the study. Among them 50 was chronic renal failure patients, remaining 50 were taken as healthy controls. The study was divided in to 2 groups. Group -1: Chronic renal failure patients, Group-2: healthy controls. The parameters plasma glucose, serum creatinine, blood urea, total protein was estimated in clinical biochemistry lab GGH by using semi-auto analyzer. Serum sialic acid was estimated by modified thiobarbituric acid method of Warren, serum malondialdehyde (MDA) was estimated by thiobarbituric acid (TBA) method. <strong>Results: </strong>The mean value of plasma glucose, blood urea, serum creatinine, serum sialic acid, serum malondialdehyde (MDA) is high in CRF patients when compared to controls. The mean value of serum total protein was low in CRF patients when compared to controls. There is a positive correlation between serum sialic acid and serum MDA(r=0.1859), serum sialic acid & serum creatinine(r=0.1462) and also a positive correlation between serum MDA and plasma glucose(r=0.164). A negative correlation was absorbed between serum sialic acid and serum total protein(r=-0.1015). <strong>Conclusion: </strong>Serum sialic acid is a marker for assessment of micro and macro vascular complications in diabetic patients. Malondialdehyde (MDA) is a biochemical marker to asses’ oxidative stress and free radical damage to the body. Screening for earliest stages of renal damage can have a significant impact on prevention and progression of diabetic nephropathy.
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Gunarsih, Arum, Pustika Amalia, and Imam Boediman. "Variables associated with malondialdehyde level in thalassemia major patients." Paediatrica Indonesiana 52, no. 3 (2012): 125. http://dx.doi.org/10.14238/pi52.3.2012.125-31.
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BackgmundThalassemia is the most cormnon hereditary haemolyticanaemia in the world, including in Indonesia. The main treatmentfor thalassemia is regular transfusions, but these are knO\vn to causeiron overload. Moreover, iron overload in jJthalassemia patientsgenerates oxygen free radicals and peroxidative lipid injury. Ferritinserum concentration is used as indirect measurement of iron overload.Malondialdehyde (MDA), a terminal compound oflipid peroxidation,is used as an index of oxidative stress status.Objective To assess the correlation between iron overload (serumferritin level) and MDA as a marker of oxidative stress in thalassemiamajor patients.Methods This c rosssectional study was conducted at CiptoMangunkusumo Hospital, Jakarta, from MayJune 2009. Subjectswere thalassemia major patients (homozygous jJthalassemia orjJthalassemia;HbE) who received regular blood transfusions, ironchelation, and vitamin E as an antioxidant. Data was collected by historytaking, physical examination, medical records, and questionnaires.Blocd specimens were dra\Vll from the thalassemia major subjects beforetransfusion and examined for serum ferritin and MDA levels.Results Fiftyfive subjects Mth thalassemia major (34 homozygousjJthalassemia and 21 jJthalassemia;HbE) were included in ourstudy. Mean serum ferritin level was 3693.2 (SD 21423),ug/L andme811 MDA level was 0.641 (SD 0.283) nmolimL. No cor relationwas found between serum ferritin and MDA levels in thalassemiamajor subjects (r=0.147, P=0.285). As additional results, this studyalso showed no correlation between MDA to reguler vitamin Econsumption (r=0.277, P=0.028) as well as MDA and nutritionalstatus (F0371, P0.()J4).Conclusion There was no cor relation between serum ferritin leveland plasma MDA level in thalassemia major subjects, no cor relationsbetween MDA and regular vitamin E consumption, as well as MDAand nutritional status. [paediatr Indones. 2012;52:125,31].
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Decroli, Eva, Asman Manaf, Syafril Syahbuddin, Yuliarni Syafrita, and Dwisari Dillasamola. "The Correlation between Malondialdehyde and Nerve Growth Factor Serum Level with Diabetic Peripheral Neuropathy Score." Open Access Macedonian Journal of Medical Sciences 7, no. 1 (2019): 103–6. http://dx.doi.org/10.3889/oamjms.2019.029.
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AIM: This study was conducted to identify malondialdehyde (MDA) serum level, nerve growth factor (NGF) serum level, diabetic peripheral neuropathy score and the correlation between MDA and NGF serum level with diabetic peripheral neuropathy score.
 METHODS: A cross-sectional study was conducted to observe diabetic patients in the internal medicine department in Dr M. Djamil Hospital, Padang, Indonesia. The MDA serum level was measured using Beuge method with thiobarbituric acid. The NGF serum level was analysed using ELISA method. Diabetic peripheral neuropathy score was defined when history score in Michigan Neuropathy Screening Instrument (MNSI) ≥ 7 and physical assessment score in MNSI > 2.
 RESULTS: Thirty subjects with diabetes has diabetic peripheral neuropathy score 3.53 (± 0.91), MDA serum level 2.16 (± 2.89) nmol/ml, and NGF serum level 10.56 (± 2.89) pg/dl. There were significant correlations between the MDA serum level and the diabetic peripheral neuropathy score (r = 0.364, p = 0.048), and between the NGF serum level with the diabetic peripheral neuropathy score (r = -0.59, p = 0.001).
 CONCLUSION: There are high MDA serum level and low NGF serum level in patients with diabetic peripheral neuropathy. Low NGF serum level plays a bigger role than high MDA serum level in diabetic peripheral neuropathy.
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Mahmoud, Tayfoor. "The Effect of Regular Exercise Training on Serum Level of Malondialdehyde." Zanco Journal of Medical Sciences 13, no. 1 (2009): 6–11. http://dx.doi.org/10.15218/zjms.2009.002.
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Background and objective: Poly unsaturated fatty acids(PUFAs ) that contain two or more double bonds are particularly susceptible to oxidation by free radicals and other highly reactive species. Malondialdehyde( MDA) is one of many low molecular weight endproducts of lipid hydroperoxide decomposition and is the most often measured as an index of lipid peroxidation. The aim of the present study was to measure the serum level of MDA in healthy non athletics and athletics. Methods : This study was carried out during the period from April 2007 to September 2007 on 53 healthy non athletics (27 males and 26 females), and 31 healthy athletics (16 males and 15 females). Serum MDA level was measured colorimetrically using thiobarbituric acid method . Results: The mean value of serum MDA was significantly higher in healthy athletics than that of healthy non athletics (p<0.05). The mean value of serum MDA in females was non significantly higher than that of males (p>0.05) in both groups . The mean value of serum MDA was significantly higher in healthy non athletic smokers than that of non smokers (p<0.001) . Conclusion: Based on the findings of the present study , it can be concluded that regular exercise training causes excess lipid peroxidation and generation of significant amounts of MDA, one of the most important harmful free radicals .Therefore athletics should take a diet rich in antioxidants or appropriate amount of antioxidant vitamins ( A, C,and E) .
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Nasruddin, Nina Indriyani, Novi Silvia Hardiany, and Wiji Lestari. "Flavonoid intake and its correlation to malondialdehyde serum among reproductive-aged women with obesity." World Nutrition Journal 5, no. i2 (2022): 8–17. http://dx.doi.org/10.25220/wnj.v05.i2.0003.
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Background: Obesity modulates oxidative stress and can be detected by examining malondialdehyde (MDA) serum levels. Flavonoids are natural bioactive ingredients that can be found in various types of vegetables and fruits that function as antioxidants to suppress that oxidative stress.
 Objective: This study aimed to determine the correlation between flavonoid intake and MDA serum levels in obese women of reproductive age.
 Methods: This cross-sectional study was conducted in Kendari, Indonesia from April to October 2021. The purposive sampling method was used to obtain 88 subjects who met the research criteria. Data were collected through interviews covering flavonoids intake using validated SQ-FFQ. Anthropometric measurements were performed to assess nutritional status, and laboratory tests were applied to determine MDA serum levels.
 Results: The average intake of flavonoids in the subjects was 142,26 ± 56,53 mg per day. Meanwhile, the average MDA serum level in the subjects was 2.16 mol/L, ranging from 1.09 nmol/ml to 6.71 nmol/ml. There was no significant correlation between total flavonoid intake and MDA serum levels in obese women of reproductive age (r=0,188, p=0,079). However, there was a weak correlation between the intake of flavonoid subclasses, namely flavan-3-ols/flavanols and MDA serum levels (r=0.325, p=0.002).
 Conclusion: We conclude that there was no correlation between total flavonoid intake and MDA serum levels. However, there was a correlation between flavan-3-ols/flavanols and MDA serum levels in the subjects.
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Pratama, Dyah Ayu Oktavianie, Zulfa Aulia, Aulanni'am Aulanni'am, and Fajar Shodiq Permata. "THE STUDY OF ORGANOPHOSPHATE (DIAZINON) TOXICITY TOWARD LIVER HISTOPATHOLOGY AND MALONDIALDEHYDE (MDA) SERUM LEVELS ON RATS (Rattus norvegicus)." Veterinary Biomedical and Clinical Journal 1, no. 2 (2019): 15–23. http://dx.doi.org/10.21776/ub.vetbioclinj.2019.001.02.3.
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Diazinon is an insecticide that has a higher toxicity than other insecticides. Normally, insecticides are detoxified by liver, but this process produced free radicals which causes cell damage. Free radicals also increase lipid peroxidation which directly increase malondialdehyde levels. This research was aimed to determine the influence of diazinon toxicity to the liver histopathology and levels of malondialdehyde (MDA) in the serum of rats (Rattus norvegicus). The rats were divided into 4 groups which consist of negative control group and three experimental groups which were given diazinon 20 mg/kgBW (P1), 40 mg/ kgBW (P2), and 60 mg/ kgBW (P3). The rats were given diazinon for 8 weeks orally. The parameters used in this research was liver histopathology with hematoxilin eosin stain and Malondialdehyde levels with Thiobarbituric test. Liver histopathology was analyzed in descriptive qualitative and the level of MDA was analyzed quantitatively using ANOVA and Tukey's exact test with α = 0.05. The result of this research showed the influence of organophosphate (diazinon) on liver histolopathology was shown by inflammatory cells infiltration in hepatic parenchyma, sinusoidal congestion, and cytoplasmic vacuolation of the hepatocytes. Statistical analysis proved that the diazinon was able to increase the level of MDA serum significantly (p<0,05) up to 71% in experimental group P1 (dose 20 mg/kgBW), which the increase level of MDA serum depend on dose of diazinon. This research concluded that diazinon is one of the organophosphate pesticide that toxic based on liver histopathology and MDA serum levels.
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Merino de Paz, Nayra, Juan Carlos Quevedo-Abeledo, Fuensanta Gómez-Bernal, et al. "Malondialdehyde Serum Levels in a Full Characterized Series of 430 Rheumatoid Arthritis Patients." Journal of Clinical Medicine 13, no. 3 (2024): 901. http://dx.doi.org/10.3390/jcm13030901.
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Background. Oxidative stress has been involved in the pathogenesis of rheumatoid arthritis (RA). The serum malondialdehyde (MDA) level is a reliable biomarker of oxidative stress status. In the present work, we aimed to analyze how a comprehensive characterization of the disease characteristics in RA, including a lipid profile, insulin resistance, and subclinical atherosclerosis, relates to serum MDA levels. Methods. In a cross-sectional study that included 430 RA patients, serum MDA levels were evaluated. Multivariable analysis was performed to examine the relationship of MDA with disease activity scores and disease characteristics, including subclinical carotid atherosclerosis, a comprehensive lipid molecule profile, and indices of insulin resistance and beta cell function indices. Results. The erythrocyte sedimentation rate (ESR) showed a significant and positive relationship with MDA. However, this did not occur for other acute phase reactants such as C-reactive protein or interleukin-6. Although the DAS28-ESR score (Disease Activity Score in 28 joints) had a positive and significant association with MDA serum levels, other disease activity scores that do not use the erythrocyte sedimentation rate in their formula did not show a significant relationship with MDA. Other disease characteristics, such as disease duration and the existence of rheumatoid factor and antibodies against citrullinated protein, were not related to serum MDA levels. This also occurred for lipid profiles, insulin resistance indices, and subclinical carotid atherosclerosis, for which no associations with circulating MDA were found. Conclusions. The disease characteristics are not related to circulating MDA levels in patients with RA.
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Verma, Vinita, Hina Oza, Riddhi Thaker, and Sunil Kumar. "Interleukin-6 and Malondialdehyde in Women with Preterm Birth." Current Immunology Reviews 15, no. 2 (2019): 207–12. http://dx.doi.org/10.2174/1573395515666191026140006.
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Background: Preterm Birth (PTB) is one of the main causes of neonatal death and infant mortality and morbidity. The pro-inflammatory cytokine interleukin-6 (IL-6) is a major proinflammatory mediator of the host response to infection and malondialdehyde (MDA) is a marker of oxidative stress. Objective : To evaluate potential associations between IL-6 and MDA levels in women with preterm birth. Method: A total of 150 women (66 with full-term and 84 with PTB) were enrolled in this case-control study. Predesigned performas were filled through questionnaire interviews to collect data on personal, demographic, occupational, lifestyle and reproductive history. Blood samples were collected within 36 hours of delivery. Serum concentrations of IL-6 and MDA were determined in mothers with full-term and preterm birth. Results: The mean age was marginally higher; whereas BMI was slightly lower in cases (PTB) as compared to controls (full-term) subjects. Serum IL-6 and MDA levels were significantly higher in subjects with PTB than full-term birth. The data were further analyzed with respect to underweight, normal and overweight/obese BMI. In all the BMI categories, the levels of IL-6 and MDA were higher in PTB cases. Among the PTB categories, the levels of IL-6 and MDA were highest in moderate to late preterm birth. A significant positive correlation was found between IL-6 and MDA levels. There was a weak negative correlation between either IL-6 or MDA and the number of gestational weeks. Conclusion : Elevated maternal serum levels of Interleukin-6 and Malondialdehyde in preterm as compared to full-term birth might suggest that inflammation and oxidative stress play a critical role in PTB.
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Salih Hamad, Mahde, Salim Jasim Khalaf, and Entedhar Riffat Sarhat. "Relationship between malondialdehyde activity (MDA) &Lipid Profile in Diabetic Patients." Tikrit Journal of Pharmaceutical Sciences 5, no. 1 (2023): 27–32. http://dx.doi.org/10.25130/tjphs.2009.5.4.27.32.
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The current investigation was designed to seek for the relationship of malondialdehyde (MDA) with the parameters of serum lipid profile in diabetes mellitus. To achieve this purpose, 55 diabetics (l I IDDM patients and 44 NIDDM patients) and 25 age matched healthy subjects were enrolled. lt was found that significant (P<0.001) elevations of MDA, S.Tc, S.Tg, LDl-cholesterol and VLDLtriglycerides in both types of diabetics when compared with those of the healthy individuals.The correlation analysis established marked conelation of MDA with S.Tc, STg, and LDl-cholesterol levels in two types of diabetics while VLDLtriglycerides demonstrated obvious correlation with MDA in IDDM. The result pointed out the interrelation ships of MDA with serum lipid profile in diabetes mellitus.
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Kamilah Mulhimah, Tisa, Dian Lestari, Woki Bilyaro, and Jhonatan Anugrah Lase. "PENGARUH KUNYIT DAN CABAI DALAM PAKAN YANG MENGANDUNG MINYAK JELANTAH TERHADAP MALONDIALDEHYDE DARAH BROILER." Journal of Agriculture and Animal Science 3, no. 1 (2023): 18–27. http://dx.doi.org/10.47637/agrimals.v3i1.729.
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Penelitian ini bertujuan mengkaji gambaran kadar malondialdehyde acid (MDA) darah ayam broiler yang diberi tepung kunyit dan cabai merah dalam pakan yang mengandung minyak jelantah. Penelitian menggunakan Rancangan Acak Lengkap (RAL) faktorial 2x3 dengan 3 ulangan, faktor pertama pemberian tepung kunyit (0% dan 0,016%) dan faktor kedua pemberian tepung cabai merah (0%, 0,5%, dan 0,75%). Total ayam yang digunakan sebanyak 180 ekor. Peubah yang diamati yaitu bobot badan, mortalitas, dan malondialdehyde acid (MDA) dalam serum darah. Data yang diperoleh dianalisis ragam (ANOVA) dan Tukey. Hasil penelitian menunjukkan adanya aktivitas antioksidan dan ada interaksi antara tepung kunyit dan cabai merah. Pemberian tepung kunyit 0,016% dan tepung cabai merah 0,75% menghasilkan kadar MDA dalam serum darah lebih rendah dari kontrol. Rendahnya MDA dalam serum darah ayam broiler yang diberi tepung kunyit dan tepung cabai merah menunjukkan adanya aktivitas antioksidan yang mempengaruhi kadar MDA.
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Ozenirler, Seren, Banu Sancak, and Ugur Coskun. "Serum and Ascitic Fluid Superoxide Dismutase and Malondialdehyde Levels in Patients with Cirrhosis." Biomarker Insights 3 (January 2008): BMI.S639. http://dx.doi.org/10.4137/bmi.s639.
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Serum and ascitic fluid superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured in 43 patients with cirrhosis and in a 10 healthy control group. Compensated cirrhotic patients had no clinically detectable ascites, but decompensated patients had massive ascites. Cirrhotic patients were divided into three groups: patients with compensated cirrhosis (n = 16), patients with decompensated cirrhosis with Spontaneous bacterial peritonitis (SBP) (n = 14), and patients with decompensated cirrhosis without SBP (n = 13). All cirrhotic patients in the experimental group had significantly higher serum SOD (p < 0.001) and MDA levels (p < 0.01) than those in the control group. There were no significant differences with respect to serum SOD and MDA levels among the three different groups of patients. There was no remarkable difference in ascitic fluid SOD and MDA levels between decompensated cirrhotic patients with and without SBP (p > 0.05). These results suggest that the increase in serum SOD and MDA levels are not related to the presence of SBP and the status of liver cirrhosis. To sum up, clarifying the impact of increased serum SOD and MDA levels in cirrhotic patients needs further investigation.
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Ashar, Yulia Khairina. "SERUM MALONDIALDEHID PADA OBESITAS DAN NON OBESITAS." Jurnal Kesehatan Tambusai 4, no. 4 (2023): 7053–57. http://dx.doi.org/10.31004/jkt.v4i4.22473.
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Obesitas dapat meningkatkan masalah kesehatan masyarakat karena merupakan faktor penyebab utama dalam pengembangan berbagai penyakit seperti dislipidemia, aterosklerosis, kardiovaskular dan lainnya. Hal tersebut terjadi melalui peningkatan produksi Reactive Oxygen Species (ROS). Peningkatan produksi ROS menyebabkan stres oksidatif hingga kerusakan sel. Stres oksidatif dalam tubuh dapat dilihat dari kadar Malondialdehyde (MDA) serum. Penelitian ini bertujuan untuk mengetahui hubungan obesitas dengan kadar MDA serum orang yang obesitas dan non – obesitas. Jenis penelitian menggunakan desain case control study. Subjek penelitian berjumlah 35 orang yang dibagi menjadi dua kelompok, yaitu orang yang obesitas dan non – obesitas. Pengukuran kadar MDA serum dilakukan dengan metode TBARs (Thiobarbituric Acid and Reactive Substances) dengan spektrofotometri, selanjutnya data dianalisis dengan menggunakan uji T-Test Independent. Penelitian ini menghasilkan rata-rata kadar MDA serum yang obesitas adalah 1,7 ± 0,4 nmol/ml dan rata-rata kadar MDA serum yang non – obesitas adalah 1,5 ± 0,5 nmol/ml. Hal ini menunjukkan orang yang obesitas memiliki kadar MDA yang lebih tinggi dibandingkan orang yang non – obesitas, dengan nilai p=0,200 (p>0,05) yang artinya tidak terdapat perbedaan yang signifikan rata-rata kadar MDA serum antara orang yang obesitas dengan non – obesitas. Kesimpulan dari penelitian ini adalah tidak terdapat perbedaan yang signifikan rata-rata kadar MDA serum antara obesitas dengan non – obesitas.
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Kartavenka, Kostya, Parinya Panuwet, Volha Yakimavets, et al. "LC-MS Quantification of Malondialdehyde-Dansylhydrazine Derivatives in Urine and Serum Samples." Journal of Analytical Toxicology 44, no. 5 (2020): 470–81. http://dx.doi.org/10.1093/jat/bkz112.
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Abstract We developed a robust analytical method for quantification of malondialdehyde (MDA) in urine and serum samples using dansylhydrazine (DH) as a derivatizing reagent. The derivatization procedure was partially carried out using an autosampler injection program to minimize errors associated with the low-volume addition of reagents and was optimized to yield a stable hydrazone derivative of MDA and its labeled d2-MDA analogue. The target MDA-DH derivatives were separated on an Agilent Zorbax Eclipse Plus Phenyl-Hexyl (3.0 × 100 mm, 3.5 μm) column. The mass-to-charge ratios of the target derivatives [(M+H)+ of 302 and 304 for MDA-DH and d2-MDA-DH, respectively] were analyzed in single ion monitoring mode using a single quadrupole mass spectrometer operated under positive electrospray ionization. The method limits of quantification were 5.63 nM (or 0.405 ng/mL) for urine analysis and 5.68 nM (or 0.409 ng/mL) for serum analysis. The quantification range for urine analysis was 5.63–500 nM (0.405–36.0 ng/mL) while the quantification range for serum analysis was 5.68–341 nM (0.409–24.6 ng/mL). The method showed good relative recoveries (98–103%), good accuracies (92–98%), and acceptable precisions (relative standard deviations 1.8–7.3% for inter-day precision; 1.8–6.1% for intra-day precision) as observed from the repeat analysis of quality control samples prepared at different concentrations. The method was used to measure MDA in individual urine samples (n = 287) and de-identified archived serum samples (n = 22) to assess the overall performance of the method. The results demonstrated that our method is capable of measuring urinary and serum levels of MDA, allowing its future application in epidemiologic investigations.
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Parahita, Astra, Galuh Hardaningsih, and Anindita Soetadji. "The Association between Serum Malondialdehyde and Nitric Oxide Level of Children Living In Area of Chronic Pesticide Exposure." Diponegoro International Medical Journal 1, no. 1 (2020): 10–15. http://dx.doi.org/10.14710/dimj.v1i1.7897.
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Introduction: Chronic exposure of organophosphate pesticides is an oxidative stress that causes liver and aortic damage. Malondialdehyde (MDA) is a biological marker of oxidative damage to cell lipids membrane. The liver produces insulin growth factor-1 (IGF-1) which stimulates the enzyme nitric oxide synthase (eNOS) to produce vascular nitric oxide (NO). Children who are living in those area may be exposed to pesticide chronically. The aim of this study is to determine the relationship between serum MDA and NO level of children living in an area of chronic pesticide exposure.Method: Cross sectional study was conducted to 50 children aged between 8 – 10 years in agriculture areas of Brebes. Serum MDA and NO level was measured at the same time using ELISA method, data were shown on numerical scale. Statistical analysisby Pearson correlation.Result: Fifty children met the criteria, consisting of 30 males (60%) and 20 females (40%). The mean of serum MDA level in males, females, and total subjects were normal 6.03 (3.86) µg/ mL, 5.18 (2.11) µg/ mL, and 5.69 (2.60) µg/ mL, respectively. The mean of serum NO level in males, females, and total subjects were increased 79.42 (50.78) µmol/ L, 68.11 (50.81) µmol/ L, and 74.90 (50.58) µmol/ L, respectively. There was no association between serum MDA and NO level found.Conclusion: Serum NO level of children living in an area of chronic pesticide exposure was higher than normal value. However, there was no association between serum MDA and NO level found.Key words: pesticides, oxidative stress, malondialdehyde, nitric oxide
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Lepara, Zahid, Orhan Lepara, Almir Fajkić, Damir Rebić, Jasmin Alić, and Hajrudin Spahović. "Serum malondialdehyde (MDA) level as a potential biomarker of cancer progression for patients with bladder cancer." Romanian Journal of Internal Medicine 58, no. 3 (2020): 146–52. http://dx.doi.org/10.2478/rjim-2020-0008.
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AbstractIntroduction. Bladder cancer is the most common malignancy involving the urinary system. Recent research tends to emphasize the role of oxidative stress products in the carcinogenesis of bladder cancer. The level of oxidative stress can be measured by assessing the MDA levels. This study aimed to evaluate serum MDA levels in patients with bladder cancer, as well as to determine its potential role as a biomarker in the diagnosis of the disease and progression risk considerations.Methods. The study was designed as a cross-sectional study and included 90 patients, divided into three groups with 30 patients each: Ta, T1and T2–T4 group, based on histopathological findings after transurethral resection of the tumor. The control group included 30 healthy volunteers. MDA level was determined using the spectrophotometric method.Results. Serum MDA level in patients with bladder cancer [0.86 (0.78–1.05) μmol/L] was significantly higher than the serum MDA level in control group [0.70 (0.69–0.72) μmol/L] (p < 0.001). Serum MDA level in Ta group [0.73 (0.70–1.05) μmol/L], T1 group [0.85 (0.80–1.12) μmol/L] and in T2–T4 group [0.91 (0.84–1.04) μmol/L] was significantly higher than the serum MDA level in control group [0.70 (0.69–0.72) μmol/L] (p < 0.01). MDA level in T1 and T2–T4 group was significantly higher than the MDA level in Ta group (p < 0.01). No significant difference was observed in MDA level between T1 and T2–T4 group (p = NS). A statistically significant positive correlation was found between tumor size and serum MDA level in patients with bladder cancer (rho = 0.254 p < 0.01).Conclusions. The results of the present study suggest that MDA serum level might play a significant role as a biomarker in the diagnosis of bladder cancer, as well as in the monitoring of its progression.
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Matsuura, Toshiyuki, Kei Takayama, Hiroki Kaneko, et al. "Nutritional Supplementation Inhibits the Increase in Serum Malondialdehyde in Patients with Wet Age-Related Macular Degeneration." Oxidative Medicine and Cellular Longevity 2017 (2017): 1–9. http://dx.doi.org/10.1155/2017/9548767.
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Purpose. To compare serum levels of malondialdehyde (MDA) in patients with wet age-related macular degeneration (wAMD), patients with dry AMD (dAMD), and patients without AMD and to evaluate the efficacy of nutritional supplementation for treating elevated serum MDA in patients with wAMD. Methods. MDA levels were measured in sera from 20 patients with wAMD, 20 with dAMD, and 24 without AMD. Patients with wAMD were randomized to receive or not receive nutritional supplementation (10 patients in each group), and MDA levels were measured after 3 months of treatment. Results. MDA levels in patients with wAMD were significantly greater compared with patients without AMD. In eyes with wAMD, there was a significant correlation between MDA levels and choroidal neovascularization lesion area. Serum MDA levels decreased in most patients that received supplementation and significantly increased in those who did not. Conclusion. Baseline serum MDA levels were elevated in patients with wAMD, and MDA levels were directly correlated with choroidal neovascularization lesion area. In addition, nutritional supplementation appeared to exert a protective effect against oxidative stress in patients with wAMD.
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Khajehnasiri, Farahnaz, Seyed Bagher Mortazavi, Abdolamir Allameh, Shahin Akhondzadeh, and Hassan Hashemi. "Total Antioxidant Capacity and Malondialdehyde in Depressive Rotational Shift Workers." Journal of Environmental and Public Health 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/150693.
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Shift work is associated with sleep deprivation, occupational stress, and increased risk of depression. Depressed patients show increased oxidative stress. During excessive oxidative stress, Malondialdehyde (MDA) increases and total antioxidant capacity (TAC) decreases in body. This cross-sectional study was conducted to determine the serum level of TAC and MDA among depressed rotational shift workers in Shahid Tondooyan Tehran Oil Refinery. 21-item Beck Depression Inventory was used to measure depression level. The level of TAC and MDA was measured by 8 mL fasting blood sample. MDA was determined by thiobarbituric acid reaction. Serum total antioxidants were measured using the ABTS. Results of this study showed that TAC mean and standard deviation concentration was 2.451 (±0.536) mg/dL and MDA was 3.725 (±1.098) mic·mol/L, and mean and standard deviation of depression score and BMI were 14.07 (±3.84) and 24.92 (±3.65) kg/m2, respectively. Depression score had a positive correlation with rotational shift work experience and work experience (r=0.218andr=0.212), respectively, (P<0.05).
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Dewi, Puspita Kusuma, Maharani Cahyono, Riski Prihatningtias, Liana Ekowati, and Arief Wildan. "The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models." Journal of Biomedicine and Translational Research 6, no. 3 (2020): 86–88. http://dx.doi.org/10.14710/jbtr.v6i3.9414.
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Introduction Retinopathy of prematurity (ROP) is the leading cause of blindness in newborn babies worldwide. The benefit of anti-oxidant was investigated for ROP cases by assessing its effect on the oxidative stress of the tissues. Glutathione is a primary endogenous in human body and its supplementation has been discovered for its benefits towards some ocular diseases. This study aims to understand the effect of glutathione on oxidative stress marker, serum Malondialdehyde (MDA), in ROP rat models.Materials and methods This was an experimental study with post test only controlled group design. Sixteen Wistar rats that met our study criterias were divided into two groups, study group and control group. The study group were exposed to 95% oxygen for 4 hours / day followed by normoxic laboratory condition for 20 hours. Glutathione 1.5 mg / day were injected intramuscularly to rats in study group. The control group was exposed to 95% oxygen followed by normoxic laboratory condition with the same manner, and did not received glutathione. This cycle was repeated for 14 days. Both groups were settled in a room temperature settings on days 15-22. Serum sampel was collected from retroorbital vein. The malondialdehyde level was analyzed using MDA analyser kit.Results MDA level was found significantly higher in study group compared to control group (546.99 ng/ml vs 201.51 ng/ml, respectively, p 0,001).Conclusion Our study demonstrated a higher MDA levels in ROP rat models given glutathione injection compared to the control group.
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Utami, Annisa Nadia, Risahmawati ., and Muniroh . "The Effect of Ramadan Fasting on Serum Malondialdehyde Levels as Indicator of Oxidative Stress and Cellular Damage." Avicenna Medical Journal 3, no. 1 (2022): 7–10. http://dx.doi.org/10.15408/avicenna.v3i1.19716.
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Background: Ramadan fasting is one of five pillars of Islam. Fasting is to refrain from eating and drinking from sunrise (sahur) to sunset (ifthar). Ramadan fasting has been shown to impact on body systems in different manners. One of the benefits of Ramadan fasting is protection against oxidative stress and cellular damage. Malondialdehyde (MDA) is a product of free radicals (hydroxil radicals) with polyunsaturated fatty acids (PUFAs) produced from lipid peroxidation of cell membranes. This paper aims to determine the effect of Ramadan fasting on serum MDA levels.Methods: The design of this study is quasiexperimental with one group pre and post design. The sample of this study was 16 preclinical students of FK UIN Syarif Hidayatullah Jakarta who performed Ramadan fasting for 17 consecutive days. Blood samples were taken 1 day before Ramadan fasting and 18 days of Ramadan fasting to measure serum MDA levels. The study was conducted in the biochemical laboratory of FK UIN Syarif Hidayatullah.Results: The mean serum MDA levels before fasting was 0.90 ± 0.54 nmol/mL. The median value of serum MDA levels after fasting was 0.43 nmol/mL, with a range between 0.03 - 2.75 nmol/mL. Ramadan fasting decreases serum MDA levels, but the decrease that occurs on the 17th day of Ramadan fasting was not significant (p> 0.05). There was an increase in serum MDA levels in 4 subjects (25%) after Ramadan fasting.Conclusion: Ramadan fasting can reduce serum MDA levels as a marker of oxidative stress and cell damage. Further study is needed to investigate the various results in serum MDA levels after fasting.
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Liu, Wei-Nung, Yi-Chiung Hsu, Chia-Wen Lu, Ssu-Chin Lin, Tsung-Jui Wu, and Gen-Min Lin. "Serum Malondialdehyde-Modified Low-Density Lipoprotein as a Risk Marker for Peripheral Arterial Stiffness in Maintenance Hemodialysis Patients." Medicina 60, no. 5 (2024): 697. http://dx.doi.org/10.3390/medicina60050697.
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Background and Objectives: Peripheral arterial stiffness (PAS), assessed by brachial-ankle pulse wave velocity (baPWV), is an independent biomarker of cardiovascular diseases (CVD) in patients on maintenance hemodialysis (HD). Malondialdehyde-modified low-density lipoprotein (MDA-LDL), an oxidative stress marker, has been linked to atherosclerosis and CVD. However, the association between serum MDA-LDL and PAS among HD patients has not been fully elucidated. This study aimed to examine the association of serum MDA-LDL with PAS in HD patients and to identify the optimal cutoff value of serum MDA-LDL for predicting PAS. Materials and Methods: A cross-sectional study was conducted in 100 HD patients. Serum MDA-LDL was quantified using an enzyme-linked immunosorbent assay (ELISA), and baPWV was measured using a volume plethysmographic device. Patients were divided into the PAS group (baPWV > 18.0 m/s) and the non-PAS group (baPWV ≤ 18.0 m/s). The associations of baPWV and other clinical and biochemical parameters with serum MDA-LDL were assessed by multivariable logistic regression analyses. A receiver operating characteristic (ROC) curve analysis was performed to determine the optimal cutoff value of serum MDA-LDL for predicting PAS. Results: In multivariable logistic regression analysis, higher serum MDA-LDL, older age, and higher serum C-reactive protein [odds ratios (ORs) and 95% confidence intervals: 1.014 (1.004–1.025), 1.044 (1.004–1.085) and 3.697 (1.149–11.893)] were significantly associated with PAS. In the ROC curve analysis, the optimal cutoff value of MDA-LDL for predicting PAS was 80.91 mg/dL, with a sensitivity of 79.25% and a specificity of 59.57%. Conclusions: Greater serum MDA-LDL levels, particularly ≥80.91 mg/dL, were independently associated with PAS in HD patients. The findings suggest that oxidative stress plays a crucial role in the pathogenesis of PAS, and targeting MDA-LDL may be a potential therapeutic strategy for reducing cardiovascular risk in HD patients.
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Carbonneau, M. A., E. Peuchant, D. Sess, P. Canioni, and M. Clerc. "Free and bound malondialdehyde measured as thiobarbituric acid adduct by HPLC in serum and plasma." Clinical Chemistry 37, no. 8 (1991): 1423–29. http://dx.doi.org/10.1093/clinchem/37.8.1423.
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Abstract Assay of free and total malondialdehyde (MDA) in human serum and plasma from healthy subjects and from patients with high risk of lipoperoxidation was performed as follows: (a) acidic (HClO4, pH 1, at 20 degrees C) or basic (NaOH, pH 13, at 60 degrees C) treatments for 30 min; (b) reaction of the protein-free extract (obtained by acid precipitation) with thiobarbituric acid (TBA); (c) HPLC separation on C18 columns with an eluting solution of methanol/phosphate buffer, 10 mmol/L, pH 5.8 (40/60, by vol), at a flow rate of 1.5 mL/min. Free MDA averaged 0.042 (SEM 0.008) and 0.043 (SEM 0.007) mumol/L, respectively, in serum and plasma from healthy subjects. Free (+/- SEM) MDA increased significantly in the plasma from cancer patients (0.270 +/- 0.047 mumol/L) and from hemodialyzed patients (0.214 +/- 0.035 mumol/L). In serum of hemodialyzed patients, analyses for total MDA were unsuitable because of interfering peaks. MDA bound to NH2 groups constituted 83.2% and 83.5% of total MDA in serum and plasma of healthy subjects, respectively, and only 58% in plasma of hemodialyzed patients.
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Pahrul, Dedi, Irfannuddin Irfannuddin, and Swanny Swanny. "Paparan Gas Amonia Karet Terhadap Perubahan Kadar Serum MDA(Malondialdehyde)." Biomedical Journal of Indonesia 3, no. 3 (2019): 113–19. http://dx.doi.org/10.32539/bji.v3i3.8601.
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Di Indonesia, amonia sudah dikenal luas sebagai bahan baku yang merupakan komoditas yang penting dalam perindustrian salah satunya yaitu industri crumb rubber (karet remah). Amonia adalah gas tajam yang tidak berwarna terdiri dari satu unsur nitrogen (N) dan tiga unsur hiodrogen (H3) dengan titik didih -33,5oC cairannya mempunyai panas penguapan yang bebas yaitu 1,37 Kj/g pada titik didihnya. Amonia secara langsung merangsang stres oksidatif dan nitrosatif pada astrosit melalui peningkatan kalsium intraselular yang menyebabkan disfungsi mitokondria dan kegagalan produksi energi selular melalui pembukaan pori-pori transisi mitokondria.. Tujuan penelitian ini adalah unt uk mengetahui pengaruh paparan gas amonia karet terhadap perubahan kadar MDA pada kelompok berisiko di Kota Palembang. Metode: design Cross Sectional. Jumlah sampel yang digunakan dalam penelitian ini sebanyak 22 orang di Kelurahan Karang Anyar Palembng dan 22 di Desa Pajar Bulan Ogan Ilir. Pemeriksaan kadar udara ambient oleh Petugas BTKL Palembang dan untuk pemeriksaan kadar serum MDA diperiksa di Laboratorium Biomolekuler FK Unsri Palembang. Data dianalisis menggunakan uji alternatif Mann Whitney. Hasil: Kadar udara ambient amonia di Kelurahan Karang Anyar sebesar 2,18 ppm lebih tinggi di bandingkan di Desa Pajar Bulan sebesar 0,0020 ppm. Rerata kadar serum MDA pada kelompok berisiko (28.7 ng/ml) lebih rendah dibandingkan kelompok tidak berisiko (35.2 ng/ml) dengan p=0.173 Kesimpulan: Tidak Terdapat Perbedaan Rerata
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Sakuraba, K., A. Krishnamurthy, A. Circiumaru, et al. "SAT0017 METABOLIC CHANGES INDUCED BY ANTI-MALONDIALDEHYDE/MALINDIALDEHYDE-ACETALDEHYDE ANTIBODIES PROMOTE OSTEOCLAST DEVELOPMENT." Annals of the Rheumatic Diseases 79, Suppl 1 (2020): 938.2–939. http://dx.doi.org/10.1136/annrheumdis-2020-eular.5013.
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Background:Malondialdehyde (MDA) is a highly reactive compound produced by lipid-peroxidation in situations associated with oxidative stress. MDA can irreversibly modify proteins residues such as lysine, arginine and histidine. In addition, MDA adducts can further react with acetaldehyde to generate malondialdehyde-acetaldehyde (MAA) modifications. Such modifications can give rise to immunogenic neo-epitopes that are recognized by autoantibodies. In fact, anti-MDA/MAA IgG antibodies are significantly increased in the serum of patients with autoimmune diseases, such as rheumatoid arthritis (RA) (1) and systemic lupus erythematosus (2). Recently, we have shown that anti-MDA/MAA IgG antibodies are able to promote osteoclast (OC) differentiationin vitro(1).Objectives:To investigate the molecular mechanisms triggered by anti-MDA/MAA autoantibodies during osteoclastogenesis.Methods:OCs were generated from monocyte-derived macrophages in the presence of the cytokines RANK-L and M-CSF. The development of OCs was monitored by light microscopy following tartrate-resistant acid phosphatase (TRAP) staining and erosion area on synthetic calcium phosphate-coated plates. Three different recombinant human monoclonal anti-MDA/MAA antibodies, cloned from single synovial B cells of RA patients, control antibodies and Fab fragments of the antibodies were added to OC cultures. Glycolysis was inhibited by 2-deoxyglucose, and Fc-gamma receptor I or II by anti-CD64 or anti-CD16 neutralizing antibodies. IL-8 levels were measured by enzyme linked immunosorbent assay. Cellular metabolism was monitored using Seahorse XF Analyzer (extracellular acidification rate and oxygen consumption) and a colorimetric L-Lactate assay.Results:Lactic acid production correlated with the osteoclastogenetic effect of some but not all anti-MDA/MAA antibodies on OCs (R=0.4758, p=0.0252) suggesting an antibody-mediated regulation of glycolysis. Further, extracellular acidification (ECAR) and oxygen consumption (OCR) rate of the developing OCs were increased by the osteoclastogenic anti-MDA/MAA clones (maximum increase of 54% for the ECAR and 78% for the OCR by clone 146+:01G07, and maximum increase of 28% for the ECAR and 39% for the OCR by clone 1103:01H05), but not by the non-osteoclastogenetic anti-MDA/MAA clones or control antibodies. The glycolysis inhibitor 2-deoxyglucose completely abolished the osteoclastogenetic effect of the anti-MDA/MAA clones at drug concentrations that did not influenced baseline OC development. Fab2 fragments of the osteocalstogenetic anti-MDA/MAA clones had no effect on OC development and metabolism. In accordance with this, Fc-gamma receptor I neutralizing antibodies completely abolished the osteocalstogenetic effect of the anti-MDA/MAA clones. The osteoclastogenetic effect of the anti-MDA/MAA antibodies was independent of IL-8 production. In contrast to anti MDA/MAA antibodies, ACPA-mediated osteoclastogenesis was independent of glycolysis and Fc-gamma receptors but dependent on IL-8.Conclusion:Our results describe a novel glycolysis-dependent mechanism by which anti-MDA/MAA antibodies promote osteoclast development that is different from the one previously described for ACPA.References:[1] C. Grönwall et al. Journal of Autoimmunity 84 (2017) 29-45.[2] C. Wang et al. Arthritis and Rheumatism 62 (2010) 2064-2072Disclosure of Interests:Koji Sakuraba: None declared, Akilan Krishnamurthy: None declared, Alexandra Circiumaru: None declared, Meng Sun: None declared, Vijay Joshua: None declared, Marianne Engström: None declared, Xiaowei Zheng: None declared, Cheng Xu: None declared, Khaled Amara: None declared, Vivianne Malmström Grant/research support from: VM has had research grants from Janssen Pharmaceutica, Sergiu-Bogdan Catrina: None declared, Caroline Grönwall: None declared, Bence Réthi: None declared, Anca Catrina: None declared
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Susiati, Asih Luklu, Ilhamjaya Patellongi, and Mushawwir Taiyeb. "ANALISIS MALONDIALDEHYDE (MDA) DAN SERUM GLUTAMIC PYRUVIC TRANSMINASE (SGPT) SETELAH BERSEPEDA 30 KM." JURNAL ILMU KEOLAHRAGAAN 22, no. 1 (2023): 43–52. https://doi.org/10.24114/jik.v22i1.45213.
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Bersepeda membuat radikal bebas meningkat sehingga kadar antioksidan berubah menyebabkan kadar Malondialdehid (MDA) dan Serum Glutamic Pyruvic Transminase (SGPT) meningkat sehingga berimplikasi pada kondisi patologi kerusakan sel. Penelitian bertujuan untuk mengetahui pengaruh umur dan kecepatan rata-rata terhadap perubahan MDA dan SGPT pada pesepeda. Penelitian ini pra-eksperimental, desain penelitian one group pretest-posttest design. Jumlah sampel dibagi menjadi 15 orang umur 30-45 dan 15 orang umur 46-60 yang memenuhi kriteria inklusi. Rerata kadar MDA kelompok umur 30-45 pretest 448,72, pada posttest 571,69 sedangkan rerata kadar SGPT pretest 20,13 posttest 25,60. Rerata kadar MDA kelompok umur 46-60 pretest 369,07, pada posttest 590,33 sedangkan rerata kadar SGPT pretest 18,80 sedangkan posttest 25,53. Hasil penelitian ada perbedaan kadar MDA dan SGPT antara sesudah bersepeda 30 km lebih tinggi secara signifikan dibanding sebelum bersepeda. Kadar MDA dan SGPT secara signifikan lebh tinggi sebelum bersepeda 30 km dibandingkan setelah bersepeda 30 km dengan kecepatan rata-rata pesepeda >20 km/jam.
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Lee, Sun Min, Young Hye Cho, Sang Yeoup Lee, et al. "Urinary Malondialdehyde Is Associated with Visceral Abdominal Obesity in Middle-Aged Men." Mediators of Inflammation 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/524291.
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The purpose of the present study was to investigate multiple anthropometric parameters used to evaluate obesity, particularly visceral abdominal fat area, and various metabolic parameters including malondialdehyde (MDA) as an oxidative stress marker. We evaluated various measures of obesity, including body mass index (BMI), waist circumference (WC), sagittal abdominal diameter, fat percentages using dual-energy X-ray absorptiometry, visceral fat area (VFA), subcutaneous fat area, multiple biomarkers related to metabolic disease, and urinary MDA, in 73 asymptomatic middle-aged men who were not severely obese. We examined relationships between multiple measures of obesity, metabolic markers, and urinary MDA levels and evaluated associations between VFA and urinary MDA. In the visceral obesity group,γ-glutamyl transferase (GGT), uric acid, and urinary MDA levels were significantly higher than in the nonvisceral obesity group (P= 0.008,P= 0.002, andP= 0.018). Urinary MDA (r= 0.357,P= 0.002) and uric acid (r= 0.263,P= 0.027) levels were only significantly positively correlated with VFA among measures of obesity. Urinary MDA, serum GGT, and serum CRP were significantly positively associated with VFA (P= 0.001,P= 0.046, andP= 0.023, resp.), even after adjusting for BMI and WC.
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Lidesna Shinta Amat, Anita, Herman Pieter Louis Wungouw, and Prisca Pakan. "The effect of robust coffee towards the malondialdehyde level in healthy adults." Journal of Medical Pharmaceutical and Allied Sciences 12, no. 4 (2023): 5983–86. http://dx.doi.org/10.55522/jmpas.v12i4.5562.
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Coffee contains more than a thousand molecules, including phenolic compounds, vitamins, minerals and alkaloids. Caffeine, cafestol, kahweol and chlorogenic acid are related to lipid metabolism and can affect serum lipid profiles. Clinical studies have reported the effects of caffeine on cardiac arrhythmias, liver function, serum cholesterol and blood pressure. Malondialdehyde (MDA) in the human body is a by-product of free radicals (oxidative stress), which can indicate whether oxidative stress is detrimental. This study aims to analyse the effect of strong coffee on MDA levels in healthy adults. This study used a quasi-experimental design with a nonequivalent control group. This design was divided into two groups: the treatment group and the control group. The treatment group was given the treatment of drinking coffee and not eating foods that can raise cholesterol. In the control group, they did not drink coffee or eat foods that could cause increased malondialdehyde levels. The results showed that coffee increased MDA levels in 91% of the samples. Thus, strong coffee can increase malondialdehyde (MDA) levels in healthy adults.
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Ayodeji, K. ADEFEMI, M. OLUMODEJI Ayokunle, O. ADEDEJI Modupe, et al. "Serum Malondialdehyde Levels in HPV-Negative and HPV-Positive DNA Tests in Women Infected with HIV in a Tertiary Hospital in Nigeria." Medical Science Research Bulletin 02, no. 02 (2025): 14–19. https://doi.org/10.5281/zenodo.14850971.
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<strong>ABSTRACT:</strong> <strong>BACKGROUND</strong> Human immunodeficiency virus (HIV) infection and human papillomavirus (HPV) are two major global health concerns, particularly in sub-Saharan Africa. Both viruses have been associated with increased oxidative stress, which can be quantified by measuring serum malondialdehyde (MDA) levels. <strong>AIM</strong> <strong>Assessing Oxidative Stress</strong>: Malondialdehyde is a biomarker for oxidative stress, which can be elevated in conditions like HIV infection. By measuring serum MDA levels, the study aims to evaluate the degree of oxidative stress in women with HIV, comparing those with and without HPV co-infection. <strong>Comparing MDA Levels in HPV Status</strong>: The study seeks to determine if there are significant differences in MDA levels between HIV-infected women who are HPV-positive and those who are HPV-negative. This could provide insights into the interaction between HPV infection and oxidative stress in the context of HIV. <strong>METHODOLOGY</strong> A total of 150 women with HIV infection who were yet to be commenced on highly active antiretroviral therapy (HAART) were enrolled, comprising 75 whom tested positive for HPV DNA and 75 tested negatives for HPV DNA test. Serum MDA levels were measured using spectrophotometry. This study employed a comparative cross-sectional approach, recruiting 150 women from LASUTH's gynaecology, colposcopy, and medical oncology clinics via convenience sampling. Fasting blood samples were collected with consent, and serum malondialdehyde levels were measured spectrophotometrically. <strong>Data Analysis</strong>: Data was analysed using statistical package of the social sciences SPSS version 29. Serum MDA levels between HPV-negative and HPV-positive groups were estimated. We also assessed the correlation between MDA levels and HPV status using regression analysis. There was a significant difference in MDA levels between HPV-negative and HPV-positive HIV-infected women. <strong>CONCLUSION</strong> Exploring serum MDA levels as a potential biomarker for cervical cancer risk assessment holds promise for improving early detection and intervention strategies. By understanding the role of oxidative stress in cervical carcinogenesis and evaluating the utility of serum MDA, healthcare professionals can develop more effective screening programs and targeted interventions to mitigate the impact of cervical cancer on women's health globally.
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Sakuraba, K., A. Krishnamurthy, A. Circiumaru, et al. "POS0400 METABOLIC CHANGES INDUCED BY ANTI-MALONDIALDEHYDE/MALINDIALDEHYDE-ACETALDEHYDE ANTIBODIES PROMOTE OSTEOCLAST DEVELOPMENT." Annals of the Rheumatic Diseases 80, Suppl 1 (2021): 429. http://dx.doi.org/10.1136/annrheumdis-2021-eular.3678.
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Background:Malondialdehyde (MDA) is a highly reactive compound generated during lipid-peroxidation in conditions associated with oxidative stress. MDA can irreversibly modify proteins (e.g. lysine, arginine and histidine residues). In addition, acetaldehyde can further react with MDA adducts to form malondialdehyde-acetaldehyde (MAA) modification. Such protein modifications can lead to immunogenic neo-epitopes that are recognized by autoantibodies. In fact, anti-MDA/MAA IgG antibodies are significantly increased in the serum of patients with autoimmune diseases, such as rheumatoid arthritis (RA) (1). Interestingly, anti-MDA/MAA antibodies have been shown to promote osteoclast (OC) differentiation in vitro suggesting a potential role for these autoantibodies in bone damage associated with RA (1).Objectives:Little is known about the molecular mechanisms activated by autoantibodies in RA. Here, we elucidate the pathways specifically triggered by anti-MDA/MAA autoantibodies in developing osteoclasts.Methods:Recombinant human monoclonal anti-MDA/MAA antibodies, which were previously cloned from single synovial B cells of RA patients, were added to different OC assays. OCs were generated from monocyte-derived macrophages in the presence of the cytokines RANK-L and M-CSF. OC development was monitored by light microscopy following tartrate-resistant acid phosphatase staining and by erosion assays using calcium phosphate-coated plates. Bone morphometrics were studied in anti-MDA/MAA-injected mice using X-ray microscopy. Cellular metabolism was analyzed by mass spectrometry, Seahorse XF Analyzer and a colorimetric L-Lactate assay.Results:Anti-MDA/MAA antibodies induced a robust OC differentiation in vitro and bone loss in vivo. The anti-MDA/MAA antibodies acted on developing OCs by increasing glycolysis via an Fcγ receptor I-mediated pathway and the upregulation of the transcription factors HIF-1α, Myc and CHREBP. Such regulation of cellular metabolism was exclusively observed in the presence of the osteoclastogenic anti-MDA/MAA clones, whereas other RA-associated autoantibodies (anti-MDA/MAA or anti-citrullinated protein antibodies) had no effect on metabolism. The anti-MDA/MAA treatment induced a shift in the tricarboxylic acid (TCA) cycle activity in developing OCs, leading to the accumulation of citrate and aconitate.Conclusion:We described a novel type of autoantibody-induced pathway in RA, which might contribute to increased OC activation and a consequent bone loss. Anti-MDA/MAA antibodies promoted osteoclast development by increasing glycolysis and by modulating the TCA cycle through a signaling pathway that included Fcγ receptor I and a network of transcription factors acting on glycolysis. A TCA cycle bias towards citrate production suggests that the anti-MDA/MAA antibodies might stimulate OCs via increasing lipid biosynthesis in the cells.References:[1]Grönwall C. et al. J. Autoimmunity 84 (2017): 29-45.Acknowledgements:This Project has received funding from FOREUM, Foundation for Research in Rheumatology, from the European Research Council (ERC) grant agreement CoG 2017 - 7722209_PREVENT RA, the EU/EFPIA Innovative Medicine Initiative grant agreement 777357_RTCure, the Konung Gustaf V:s och Drottning Victorias Frimurarestiftelse and Knut and Alice Wallenberg Foundation.Disclosure of Interests:Koji Sakuraba: None declared, Akilan Krishnamurthy: None declared, Alexandra Circiumaru: None declared, Vijay Joshua: None declared, Heidi Wähämaa: None declared, Marianne Engström: None declared, Meng Sun: None declared, Xiaowei Zheng: None declared, Cheng Xu: None declared, Khaled Amara: None declared, Vivianne Malmström Grant/research support from: collaboration with Pfizer, unrelated to the abstract, Sergiu-Bogdan Catrina: None declared, Caroline Grönwall: None declared, Bence Réthi: None declared, Anca Catrina Grant/research support from: collaboration with BMS and Pfizer, unrelated to the present abstract
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Intihar, Urska, Arta Krasniqi, Anze Djordjevic, et al. "Oxidative Stress in Postoperative Atrial Fibrillation: Does Malondialdehyde Hold Predictive Value?" Medicina 61, no. 5 (2025): 778. https://doi.org/10.3390/medicina61050778.
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Background and Objectives: Postoperative atrial fibrillation (POAF) is a common complication following cardiac surgery, associated with increased morbidity and prolonged hospital stays. Oxidative stress has been implicated in POAF pathogenesis, with malondialdehyde (MDA), a marker of lipid peroxidation, proposed as a potential biomarker. However, conflicting evidence exists regarding its predictive value. This study aimed to assess the association between serum MDA levels and POAF incidence in patients undergoing cardiac surgery. Materials and Methods: This prospective observational study included 99 consecutive patients undergoing elective on-pump cardiac surgery. Patients with preoperative atrial fibrillation, chronic kidney disease requiring dialysis, or emergency surgery were excluded. Blood samples for MDA measurement were collected at six perioperative time points: preoperatively, intraoperatively after aortic clamp release, and at 8, 24, 48, and 72 h postoperatively. Patients were monitored for new-onset POAF during the first three postoperative days. Statistical analyses included independent samples t-tests, Mann–Whitney U-tests, and Fisher’s exact tests, with significance set at p < 0.05. Results: POAF occurred in 33 (33%) patients. Patients who developed POAF were significantly older (p = 0.017) and had higher EuroSCORE II values (p = 0.019). No significant differences were observed in serum MDA concentrations between POAF and non-POAF patients at any measured time point. The incidence of POAF was higher in patients undergoing valvular surgery (p = 0.014). Conclusions: Serum MDA levels were not associated with POAF development, suggesting that lipid peroxidation alone may not play a central role in POAF pathogenesis. These findings challenge the predictive value of MDA for POAF risk stratification. Future research should explore alternative oxidative stress markers and their potential therapeutic implications in POAF prevention.
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Atik, Nur, S. Putri Pratiwi, and Laniyati Hamijoyo. "Correlation between C-reactive Protein with Malondialdehyde in Systemic Lupus Erythematosus Patients." International Journal of Rheumatology 2020 (July 1, 2020): 1–5. http://dx.doi.org/10.1155/2020/8078412.
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Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by an inflammatory process. One of the inflammation markers that can be measured is C-reactive protein (CRP). Another indicator of inflammation is malondialdehyde (MDA), though it is still uncommon to be analyzed in SLE patients. The study looked for the MDA value and found a correlation with CRP. A cross-sectional study design with a correlative analytical was performed. CRP level data was taken from Hasan Sadikin lupus registry data, and MDA levels were analyzed from a bioarchive patient’s serum. We collected the patients’ data who had CRP level from Hasan Sadikin lupus registry and analysed MDA levels from the serum sample. MDA levels were analyzed using an ELISA method. The data obtained were analyzed using the Pearson correlation and Eta correlation test. The study involved 78 data patients as subjects. It was found that the median of CRP and MDA was 0.85 mg/l and 153.10 ng/ml, respectively. These results indicate that the CRP levels in SLE patients are still within normal limits. Statistical analysis showed no correlation between CRP and MDA level (r=0.2, P>0.05). Additionally, the correlation between CRP and MDA with organ involvement, such as lupus nephritis (LN), lupus cutaneous (LC), and lupus musculoskeletal (LM), showed no correlation (Fh<Ft). There is no correlation between CRP and MDA levels in SLE patients, and specific organ involvement of the disease does not affect the correlation.
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Gubaljevic, Jasmina, Nahida Srabović, Adlija Jevrić-Čaušević, et al. "Serum levels of oxidative stress marker malondialdehyde in breast cancer patients in relation to pathohistological factors, estrogen receptors, menopausal status, and age." Journal of Health Sciences 8, no. 3 (2018): 154–61. http://dx.doi.org/10.17532/jhsci.2018.263.
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Introduction: The aim of this study was to determine the serum levels of malondialdehyde (MDA) in patients with invasive breast cancer in relation to its serum levels in patients with benign breast disease, and to investigate correlation between MDA serum levels with pathohistological prognostic factors (tumor size, lymph node involvement, and histologic grade [HG]), estrogen receptor (ER) status, and with breast cancer patient’s age and menopausal status.
 Methods: A total of 43 with well-documented invasive breast cancer were included in this study: 27 with positive axillary’s lymph nodes, and 16 with negative axillary’s lymph nodes, and 39 patients with findings of benign breast diseases. MDA determination in serum of breast cancer and benign breast disease patients was performed by the fluorimetric method, immunohistochemical staining was performed for ER, and routine pathohistological examination was conducted for pathohistological factors.
 Results: MDA serum levels in breast cancer patients were significantly higher than MDA serum levels in benign breast disease patients (p = 0.042). No statistically significant difference between MDA serum levels in breast cancer patients with and without lymph node metastases was found (p = 0.238). No statistically significant correlations between MDA serum levels and tumor size (p = 0.256), HG (p = 0.124), or number of positive lymph nodes (0.113) were found. A statistically significant correlation between serum MDA levels and ages of breast cancer patients with lymph node metastases was found (p = 0.006).
 Conclusion: Obtained results support the importance of MDA in the carcinogenesis of breast cancer. According to our findings, serum level of MDA could not be a useful prognostic factor in breast cancer.
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Nerurkar, Alka V., Shalaka S. Prabhu, Sachin A. Patharkar, et al. "Study of serum malondialdehyde and uric acid levels in patients with malaria." International Journal of Clinical Biochemistry and Research 8, no. 3 (2021): 219–21. http://dx.doi.org/10.18231/j.ijcbr.2021.045.
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Malaria is parasitic disease of humans caused by parasitic protozoan and genus plasmodium, widely present in tropical region. In the blood, the parasite travel to the liver to mature and reproduce. Oxidative stress is generated through the invasion of malarial parasites in human system. Malondialdehyde is a highly reactive compound is assayed in vivo as a biomarker of oxidative stress. Uric acid contributes to the pathology of human malaria by stimulating the production of cytokines from immune system. To estimate serum MDA & serum uric acid levels in patients with malarial infection and compare same with healthy individuals. This is a cross-sectional observational study, cases and controls were selected using random sampling method, attending hospital OPD. Study includes 50 laboratory diagnosed cases of malaria patients with equal age and sex matched controls. MDA was estimated using MDA - thiobarbituric acid method, uric acid was estimated by phosphotungstic acid method. Standardization of both the methods was carried out prior to experiment. There is generalized increase in serum MDA and uric acid levels in cases as compared to the control group.Shalaka S Prabhu, Dr. Sachin A Patharkar, Dr. Neelam J Patil, Jalinder B Sanap, Kalpana U Shinde, Rupa R Dalvi.
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Nagaraj, Satish Kumar D, and Prashant V. Paunipagar. "Study of serum malondialdehyde and vitamin c in smokers." Journal of Scientific and Innovative Research 3, no. 6 (2014): 569–71. http://dx.doi.org/10.31254/jsir.2014.3604.
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Smoking is associated with high incidence of morbidity and mortality. The free radicals released during smoking thought to play an important role in forming imbalance between oxidants and antioxidants. A total number of 200 subjects comprising of 50 healthy controls and 150 cases of smokers divided into mild, moderate and heavy smokers studied. In all the subjects, serum levels of malondialdehyde (MDA) as a biomarker of lipid peroxidation and antioxidant serum vitamin C were estimated. Serum MDA was significantly increased in smokers when compared to controls. The oxidative stress level was elevated in accordance with the intensity of smoking. The antioxidant serum vitamin C was significantly decreased in smokers when compared to controls. The presence of increased systemic oxidative stress in smokers seems to be associated with current active smoking and systemic inflammation. The decrease in antioxidant levels appears to be mainly a consequence of increased oxidative stress. Hence by advising cessation of smoking and taking diet rich in antioxidants may prevent oxidative damage and prevent oxidative stress related diseases.
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Aktas, Mustafa Sinan, Fatih Mehmet Kandemir, Akin Kirbas, Basak Hanedan, and Mehmet Akif Aydin. "Evaluation of oxidative stress in sheep infected with Psoroptes ovis using total antioxidant capacity, total oxidant status, and malondialdehyde level." Journal of Veterinary Research 61, no. 2 (2017): 197–201. http://dx.doi.org/10.1515/jvetres-2017-0025.
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AbstractIntroduction:The study aimed at evaluating oxidative stress using malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) markers in sheep naturally infected withPsoroptes ovis(Acari).Material and Methods:The study was performed on 40 sheep divided into two equal groups: a healthy group (group I) and a group naturally infected withPsoroptes ovis(group II). The sera were obtained by centrifuging blood samples collected from the vena jugularis and serum MDA level changes in the samples were measured spectrophotometrically. Commercially available test kits were used for the measurement of TAC and TOS levels. The percentage ratio of TOS level to TAC level was accepted as OSI.Results:The serum malondialdehyde, total oxidant status levels, and oxidative stress index increased significantly (P < 0.01) in group II, while the serum total antioxidant capacity levels decreased significantly (P < 0.01) in this group. Negative correlations between total antioxidant capacity and total oxidant status and total antioxidant capacity and malondialdehyde, and a positive correlation between total oxidant status and malondialdehyde were found in infected sheep.Conclusion:The obtained results indicated the relationship between oxidant/antioxidant imbalance andPsoroptes ovisinfection in sheep. Their MDA, TAC, TOS, and OSI markers may be used to determine the oxidative stress in natural infections withPsoroptes ovis.
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Firsta Yosika, Ghana, Pamuji Sukoco, Adi Pranoto, and Septyaningrum Putri Purwoto. "Penurunan malondialdehyde serum setelah latihan interval dan continuous di pagi hari pada perempuan obesitas." Jurnal SPORTIF : Jurnal Penelitian Pembelajaran 6, no. 2 (2020): 288–303. http://dx.doi.org/10.29407/js_unpgri.vi.14289.
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Penelitian ini bertujuan untuk menganalisis penurunan malondialdehyde (MDA) setelah latihan interval dan continuous di pagi hari pada perempuan obesitas. Penelitian ini adalah true experiment dengan rancangan penelitian a Basic Time Series Design dengan menggunakan subjek 27 perempuan obesitas usia 20-25 tahun, body mass index (BMI) 25-35 kg/m2, percentage body fat (PBF) di atas 30% dan VO2max 25-35 ml/kg/min dan secara random dibagi menjadi tiga kelompok, yaitu CON (n=9, kontrol tanpa intervensi), MIE (n=9, moderate interval exercise) dan MCE (n=9, moderate continuous exercise). Intervensi dilakukan di pagi hari pukul 07.00-09.00 WIB. Intervensi MIE dan MCE dilakukan selama 40 menit menggunakan treadmill. Pengambilan sampel darah dilakukan pre-exercise, 10 menit dan 6 jam post-exercise. Pengukuran serum MDA menggunakan metode Thiobarbituric Acid Reactive substance (TBARs). Teknik analisis data menggunakan uji ANOVA dan LSD post hoc test dengan Statistic Package for Social Science (SPSS) versi 21. Serum MDA menurun signifikan setelah 10 menit dan 6 jam pasca intervensi MIE dan MCE (P<0.05), tetapi tidak pada CON (P>0.05). Berdasarkan hasil penelitian disimpulkan bahwa serum MDA menurun setelah 10 menit dan 6 jam pasca intervensi MIE dan MCE dibandingkan dengan CON pada perempuan obesitas. Tetapi pola penurunan serum MDA pada MCE lebih rendah dibandingkan dengan MIE dan CON.
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Ioannidou, Stavroula, Argyrios Ginoudis, Kali Makedou, Magda Tsolaki, and Evgenia Lymperaki. "Serum and Cerebrospinal Fluid Malondialdehyde Levels in Patients with Mild Cognitive Impairment." Journal of Xenobiotics 15, no. 2 (2025): 50. https://doi.org/10.3390/jox15020050.
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Mild cognitive impairment (MCI) is recognized as an intermediate stage between normal aging and dementia. Oxidative stress is implicated in the pathophysiology of neurodegenerative diseases, playing a crucial role. This study aimed to investigate the differences in malondialdehyde (MDA) levels in the serum and cerebrospinal fluid (CSF) of patients with MCI compared to FDA-approved biomarkers, based on age, sex, and education level. Participants aged 55–90 years old were categorized into three groups based on FDA-approved biomarkers, especially the CSF Aβ42/Aβ40 ratio and clinical screening assessments: 30 MCI (A+) patients with abnormal CSF Aβ42/Aβ40 ratios (Group A), 30 MCI (A−) patients with normal CSF Aβ42/Aβ40 ratios (Group B), and 30 healthy (A−) participants with normal CSF Aβ42/Aβ40 ratios (Group C). The measurements of CSF FDA-approved biomarkers were performed using an automated immunochemical method (Fujirebio, Inc.), while MDA determination was performed using a competitive inhibition enzyme immunoassay technique (ELK Biotechnology Co., Ltd.). Our results showed that the mean CSF MDA values were significantly lower in group C than in group A (83 ng/mL vs. 130 ng/mL, p = 0.024) and group B (83 ng/mL vs. 142 ng/mL, p = 0.011), respectively. Differences in serum and CSF MDA levels were presented in the study groups based on sex, age, and education level. These findings suggest that lipid peroxidation, as indicated by CSF MDA, could serve as a potential biomarker for the early recognition of MCI.
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Yulianti, Maria Eka Patri, Elvira Yunita, Zhafran Hafizhki, Meiny Suzery, Neni Susilaningsih, and Suhartono Suhartono. "Ekstrak Labu Siam (Sechium edule) Dapat Menurunkan Kadar Serum Malondialdehid pada Tikus Putih (Rattus norvegicus) yang Diberikan Pakan Kolesterol." Journal of Telenursing (JOTING) 4, no. 1 (2022): 128–34. http://dx.doi.org/10.31539/joting.v4i1.3587.
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This study aimed to analyze the effect of chayote extract (Sechium edule) on malondialdehyde serum levels in white rats (Rattus norvegicus) fed a high-cholesterol diet and to prove the antioxidant content of chayote. The method used is experimental with a post-test-only control group design. This study used white rats, which were divided into five groups. The results of this study indicate that the administration of chayote extract at a dose of 0.25 g/kg BW and 0.5 g/kg BW can reduce the serum MDA concentration, although it is not statistically significant, while the administration of a chayote extract at a dose of 0.75 g/Kg BW can significantly reduce serum MDA levels in white rats induced by hypercholesterolemia. In conclusion, chayote extract at a dose of 0.75 g/kg BW can reduce blood MDA serum levels in white rats induced by hypercholesterolemia.
 Keywords: Chayote Extract, Hypercholesterolemia, Malondialdehyde
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Hou, Jia-Sian, Chih-Hsien Wang, Yu-Hsien Lai, et al. "Serum Malondialdehyde-Modified Low-Density Lipoprotein Is a Risk Factor for Central Arterial Stiffness in Maintenance Hemodialysis Patients." Nutrients 12, no. 7 (2020): 2160. http://dx.doi.org/10.3390/nu12072160.
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Circulating malondialdehyde-modified low-density lipoprotein (MDA-LDL) acts as a marker of oxidative stress and is associated with atherosclerotic cardiovascular disease. The relationship between serum MDA-LDL levels and aortic stiffness (AS) in patients with hemodialysis (HD) was evaluated. There were 155 HD patients enrolled in this study. Carotid-femoral pulse wave velocity (cfPWV) was measured by a validated tonometry system. Patients with cfPWV >10 m/s were used to define the AS group, while those with values of ≤10 m/s were regarded as the control group. Serum MDA-LDL levels were measured using a commercial enzyme-linked immunosorbent assay. Sixty-eight patients (43.9%) who were defined as AS sufferers, and were older, had a higher percentage of diabetes and hypertension and higher systolic blood pressure and serum MDA-LDL level compared to subjects in the control group. After adjusting for factors significantly associated with AS by multivariable logistic regression analysis, it was revealed that serum MDA-LDL levels, diabetes, and hypertension were independent predictors of AS in HD patients. Multivariable forward stepwise linear regression analysis also showed that a logarithmically transformed MDA-LDL level was significantly correlated with cfPWV values in HD patients. In HD patients, a high serum MDA-LDL level was positively associated with cfPWV values and was a significant predictor of the development of high AS.
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Zor, Ramazan Kürşad, Serpil Erşan, Erkut Küçük, Gamze Yıldırım, and İsmail Sarı. "Serum malondialdehyde, monocyte chemoattractant protein-1, and vitamin C levels in wet type age-related macular degeneration patients." Therapeutic Advances in Ophthalmology 12 (January 2020): 251584142095168. http://dx.doi.org/10.1177/2515841420951682.
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Purpose: The purpose of this study was to investigate the serum levels of malondialdehyde (MDA) which is a marker of oxidative stress, monocyte chemoattractant protein-1 (MCP-1) which has an important role in inflammation, and vitamin C which has antioxidant properties in patients with wet age-related macular degeneration (wAMD). Methods: Thirty patients with wAMD were included in the study and serum levels of MDA, MCP-1, and vitamin C were compared with healthy participants ( n = 30). Serum vitamin C and MDA levels were measured using a spectrophotometric method. Serum MCP-1 levels were determined by the ELISA method. Results: MCP-1 and MDA levels were higher in patients with wAMD compared with the control group ( p < 0.05). Serum vitamin C levels were lower in patients with wAMD compared with the control group ( p < 0.05). Conclusions: The increase in the MCP-1 levels in patients with wAMD may be associated with increased inflammation in wAMD. Decreased serum vitamin C and elevated MDA levels in patients with wAMD suggest increased oxidative stress in wAMD patients. These results indicate that the increased oxidative stress and inflammation can play a role in the pathogenesis of wAMD.
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Merino de Paz, Nayra, María García-González, Fuensanta Gómez-Bernal, et al. "Relationship between Malondialdehyde Serum Levels and Disease Features in a Full Characterized Series of 284 Patients with Systemic Lupus Erythematosus." Antioxidants 12, no. 8 (2023): 1535. http://dx.doi.org/10.3390/antiox12081535.
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Malondialdehyde (MDA) is a marker of oxidative stress and antioxidant status. Oxidative stress has been observed to be increased in systemic lupus erythematosus (SLE). Some studies have shown that MDA is upregulated in SLE compared to controls. However, the literature lacks reports regarding the relationship of MDA to disease manifestations. This is relevant since SLE is a multisystemic disease which may affect virtually any organ in the body. In this study, we set out to analyze how MDA serum levels are associated with disease expression in a large series of SLE patients who were fully characterized in clinical and laboratory terms. A total of 284 patients with SLE were recruited. Serum levels of MDA, and the activity (SLEDAI), severity (Katz) and damage index (SLICC-DI) scores, full lipid profile, and carotid subclinical atherosclerosis were assessed. In addition, a full characterization of the complement system was performed in SLE patients’ samples. Multivariable linear regression analysis was executed to study the relationship between clinical and laboratory disease characteristics and MDA. A statistically significant negative relationship was found between disease duration and MDA. In contrast, the presence of anti-nucleosome antibodies was positively associated with MDA. Regarding the SLICC-DI areas, both the musculoskeletal domain and the cutaneous domain were significantly related to higher serum MDA values. Furthermore, after adjustment for confounding factors, lower levels of the classical complement pathway, which denotes activation, were associated with higher serum levels of MDA. In conclusion, cumulative musculoskeletal and skin damage in SLE patients is associated with superior serum levels of MDA. In addition, activation of the complement system is also related to higher circulating MDA levels.
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Safithri, Mega, Kustiariyah Tarman, Iriani Setyaningsih, Yanti Fajarwati, and Imanniar Yuta Ellana Dittama. "In Vitro and In Vivo Malondialdehyde Inhibition Activities of Stichopus hermanii and Spirulina platensis." HAYATI Journal of Biosciences 29, no. 6 (2022): 771–81. http://dx.doi.org/10.4308/hjb.29.6.771-781.
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Previous research showed that Stichopus hermannii and Spirulina platensis had an antioxidant activity. It is indicated by the reduced malondialdehyde (MDA) level in the liver of the diabetic rats. However, the STZ administration did not significantly increase MDA concentration of diabetic rats' blood serum for 14 days. This research aimed to determine in vitro and in vivo MDA inhibition of S. hermanii and S. platensis. The in vitro antioxidant activity test was conducted using the MDA-TBA method, and a positive control used α-tocopherol. For in vivo experiment, diabetic rats (DM) were induced by streptozotocin for 21 days. Twenty-five rats which were divided into five groups: normal rat group (NA), diabetic rat group (DA), diabetic rat group + glibenclamide (DG), diabetic rats + Stichopus hermanii (SH), and diabetic rats + Spirulina platensis (SP). The in vitro results showed that the antioxidant activity of 25 ppm Spirulina platensis had the same MDA inhibitory activity as 200 ppm α-tocopherol, but 200 ppm Stichopus hermanii had lower inhibition than 200 ppm α-tocopherol. The in vivo result showed that Stichopus hermanii treatment was more effective in suppressing blood serum MDA concentration, but Spirulina platensis was more effective in suppressing liver MDA concentration.
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Sekunda, Kaninta Nuga, Ariosta Ariosta, Charles Limantoro, and Andreas Arie Setiawan. "Difference in Profiles of Oxidative Stress Marker (MDA) in STEMI and NSTEMI." DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) 10, no. 2 (2021): 145–50. http://dx.doi.org/10.14710/dmj.v10i2.29673.
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ABSTRACT Introduction: Acute Myocardial Infarction (AMI) results in necrosis of the myocardium due to blockage in the coronary artery. AMI is classified into two categories, STEMI and NSTEMI. AMI is a multifactorial condition closely related to the increase in production of reactive oxygen species (ROS). As the end product of lipid peroxidase, Malondialdehyde (MDA) is often used as a biomarker for oxidative stress. Purpose: To prove the difference between profiles of oxidative stress marker (MDA) in STEMI and NSTEMI. Methods: An observational analytic study with a cross-sectional study approach done in Dr. Kariadi Central Public Hospital and Diponegoro National Hospital, Semarang, Central Java, between April and September 2020. Subjects were diagnosed by the presence of chest pains and an increase in CKMB and troponin levels. The study was conducted on 47 subjects consisting of 20 STEMI samples and 27 NSTEMI samples. Serum MDA was examined using the TBARS method. Data were analyzed by a computer program. Results: Mean serum MDA in STEMI and NSTEMI was 0,22 ± 0,12 μmol/L and 0,82 ± 0,92 μmol/L respectively. From the statistical analysis, results showed that the difference in serum MDA concentrations between STEMI and NSTEMI were significant (p = 0,007). Conclusion: Malondialdehyde concentration in NSTEMI was significantly higher than in STEMI. It is suggested that further research be done to know which type of MDA is more accurate, to understand the effect of lipid profile towards STEMI and NSTEMI, and to put patient’s medical history into consideration. Key Words: Acute Myocardial Infarction, Reactive Oxygen Species, Oxidative Stress, Malondialdehyde
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Rismayanti, Levana, Yulistiani Yulistiani, Mia Ratwita Andarsini, and Mariyatul Qibtiyah. "ANALYSIS OF DL--TOCOPHEROL AS ANTIOXIDANT ON MALONDIALDEHYDE LEVEL IN PEDIATRIC PATIENTS WITH -THALASSEMIA MAJOR." Folia Medica Indonesiana 53, no. 1 (2017): 49. http://dx.doi.org/10.20473/fmi.v53i1.5490.
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Thalassemia is a hereditary form of anemia that affects the synthesis of hemoglobin. The management of therapy in patients with b-thalassemia major which patients should receive continuous blood transfusions and increased iron absorption from the digestive tract causes excess iron in the body. This will lead to an increase of free iron level that triggers Radical Oxygen Species (ROS). Increased level of ROS can initiate lipid peroxidation which used as an indicator of oxidative stress in cells and tissues and produce reactive carbonyl, mainly malondialdehyde (MDA). Thus, MDA measurement is widely used as an indicator of lipid peroxidation. On the other hand, the risk of oxidative damage can be reduced by antioxidant, one of them is Vitamin E that is a fat-soluble vitamin with high potential antioxidant. The objective of this study was to analyze the effect of the dl-a-tocopherol (Vitamin E) administration on decrease of MDA serum level on pediatric patients with b-thalassemia major. This was a longitudinal observational study design for one group without comparison was conducted to examine the use of vitamin E to decreased MDA serum level on children patients with b-thalassemia major. The inclusion criteria were patients who rely on blood transfusions, patients who received only one type of iron chelating agents during the study period, the clinical condition is stable, agrees, and has completed the informed consent. In the course of the study of 21 patients there were variations in patient compliance in taking vitamin E tablet dosage 200 IU once-daily for one month: only 11 out of 21 patients consumed 30 tablets of vitamin E 200 IU (total dose of 6000 IU) in the 1-month study, and only data from those 11 samples will be analysed further. MDA serum level was measured pre- and post-administration of vitamin E and patient’s characteristics of subjects was obtained for additional information. Pre-administration of vitamin E, serum level of MDA was 1239.4 ± 502.55 ng/mL with a range of 216.95 to 2297.3 ng/mL, whereas in the group post administration of vitamin E, MDA serum level was 786.49 ± 704.88 ng/mL with a range of 6.5380 to 1958.6 ng/mL. In conclusion, there was no significant difference in MDA serum level in the group pre- and post- administration of vitamin E (p = 0.15).
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Gulzar I. Ibrahim, Saman M. Abdulkareem, and Lutfiya M. Hasan. "Estimation of Nitric Oxide, Malondialdehyde, and Adenosine Deaminase in Serum of Hypertensive Patients and Normotensive Individuals in Erbil City." Tikrit Journal of Pure Science 24, no. 3 (2019): 17–22. http://dx.doi.org/10.25130/tjps.v24i3.364.
Full textAbstract:
This study was aimed to estimate nitric oxide (NO), malondialdehyde (MDA), and adenosine deaminase (ADA) in the serum of hypertensive patients. Fifty patients (25 males and 25 females) age 40-70 diagnosed with hypertension involved in the study. Fifty healthy individuals, who had no hypertension in the last year, were identified as the control group. NO, MDA and ADA have performed accordingly. MDA was higher with aging and gender in hypertensive patients. Serum level of MDA was higher in females compared with male due to oxidative stress more in female than a male with aging. ADA was higher among hypertensive with aging, though no significant differences among gender. Serum level of NO was lower with aging with no significant differences among gender.