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Orruño, Aguado Estibalitz. "The seed storage proteins of sesame (Sesamum indicum) in relation to food allergy." Thesis, University of Leeds, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.396938.
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Moazzami, Ali A. "Sesame seed lignans : diversity, human metabolism and bioactivities /." Uppsala : Department of Food Science, Swedish University of Agricultural Sciences, 2006. http://epsilon.slu.se/200698.pdf.
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Chien, Yung-hsin. "SHELF LIFE EXTENSION OF SEED BUTTER MADE WITH SESAME, SUNFLOWER AND PUMPKIN SEEDS." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1449235219.
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Gungor, Ugras. "Design Of A New Equipment For Sesame Seed Dehulling." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/2/678/index.pdf.
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In this study, new methods and processing equipments for sesame dehulling were investigated.
First, water absorption of sesame seed was studied at 20, 30, and 40°C. The data could be modeled using Peleg equation where it was found that the constant k1 was inversely related to temperature but the effect of temperature on k2 was negligible. In the second phase of the work a lab scale continuous screw conveyor as dehuller and two equipments, (1) fluidized bed dryer and (2) hull separator to function as agitator, dryer and separator, for hull separation were designed. Fluidized bed unit was unsuccessful as it caused rapid drying of seeds before hulls can be removed. Using designed dehuller and hull separator, seeds at 30.5, 50.4 and 70.7 % db moisture contents were processed at dehuller speeds of 420, 840, and 1150 rpm. It was found that the percentage of dehulled seeds was linearly dependent on moisture content, optimal speed of designed dehuller was 840 rpm, and results for the efficiency of dehulling the seeds were significantly the same at 420 and 1150 rpm. Repeated passes of seeds through dehuller not only increased the efficiency of dehulling but also the percentage of damaged seeds. A dehulling efficiency of about 92.5 % was attained after four passes. The possibility of soaking seeds in an enzyme solution before dehulling was also investigated. By this means, after soaking in 0.2 % (v/v) Peelzyme-I solution for 15 min, a dehulling efficiency of 95 % was achieved.
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Jacklin, Alix. "An investigation into the bioactivity of compounds within the non-oil component of sesame seed oil." Thesis, University of Hull, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.417171.
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Pires, Raquel Maria de Oliveira. "Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas a diferentes condições de estresse." Universidade Federal de Viçosa, 2014. http://locus.ufv.br/handle/123456789/4632.
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Previous issue date: 2014-02-26Conselho Nacional de Desenvolvimento Científico e Tecnológico
Seeds frequently encounter biotic and abiotic stress that adversely affects growth, development, or productivity. The objective of this work was to investigate the effect of nitric oxide (NO) like protective agent in sesame seeds submitted to different stresses. Therefore, two experiments were conducted. In the first one, sesame seeds were placed in soaked substrate with water or with different concentrations of cadmium chloride and cadmium chloride plus sodium nitroprusside (SNP) in the following treatments: 1) water (control), 2) +200 μM of SNP, 3) 800 μM of CdCl2, 4) 800 μM of CdCl2 +200 μM of SNP, 5) 600 μM of CdCl2, 6) 600 μM of CdCl2 +200 μM of SNP, 7) 400 μM of CdCl2 and 8) 400 μM of CdCl2 +200 μM of SNP. Were done the following determinations: germination (G), first count of germination (PC), speed germination index (IVG), hipocotyl length (CH), radicle length (CR), dry mass of hipocotyl (MSH) and dry mass of radicle (DMR) and imbibition curve. It were quantified the activity of antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and total peroxidase (POX). It was adopted the completely randomized design with five replicates. The data were submitted to variance analysis and the means obtained for treatments were compared by Tukey test at 5% of significance. The means obtained in treatments with and without SNP were compared by F test at 5% and the times of imbibition by Tukey test at 5%. For the realization of imbibition curve was realized a polynomial regression fit 3. In the second experiment, sesame seeds were placed in soaked substrate with water or with different concentrations of PEG 6000 and PEG 6000 plus SNP in the following treatments: 1) water (control), 2) +200 μM of SNP, 3) 0,1MPa, 4) -0,1MPa +200 μM of SNP, 5) - 0,2MPa, 6) -0,2MPa +200 μM of SNP, 7) -0,3MPa and 8) -0,3MPa, +200 μM of SNP. The same determinations done in experiment one, was made in the experiment two. Both stress caused the reduction of germination and vigor, being the highest concentrations, the most harmful to sesame seeds. NO was able to minimize the damage caused by the stresses, reversing partially all the biochemical parameters evaluated, what provides higher and faster germination, beyond the higher initial development of xiii seedlings. Cadmium and PEG 6000 provoked less absorption and less gain of mass in higher concentrations in relation to water, and an extension of Phase II. In relation to the antioxydative system, there was an increaseof enzymatic activity in period of 0 to 24 houras, showing the organization of the antioxidant system with the sesame seeds over time. Was observed that in higher potentials and in solutions more concentrated, the activity enzymatic was higher, what suggest detoxifying activity of these molecules in seeds submitted to water deficit and solution of cadmium more strict. The use of SNP, increased the activity of antioxidative enzymes, evidencing an efficient of elimination of EROs formed.
Sementes estão frequentemente expostas a diversos estresses bióticos e abióticos que prejudicam o seu crescimento, desenvolvimento e produtividade. O objetivo desse trabalho foi investigar o efeito do óxido nítrico (ON) como agente protetor em sementes de gergelim (Sesamum indicum L.) submetidas à diferentes estresses. Para tanto, foram conduzidos dois experimentos. No primeiro, as sementes foram semeadas em substrato umedecido com água ou com diferentes concentrações de cloreto de cádmio (CdCl2) e cloreto de cádmio acrescido de nitroprussiato de sódio (SNP) nos seguintes tratamentos: 1) água (controle), 2) +200 μM de SNP, 3) 800 μM de CdCl2, 4) 800 μM de CdCl2 +200 μM de SNP, 5) 600 μM de CdCl2, 6) 600 μM de CdCl2 +200 μM de SNP, 7) 400 μM de CdCl2 e 8) 400 μM de CdCl2 +200 μM de SNP. As seguintes determinações foram feitas: germinação (G), primeira contagem de germinação (PC), índice de velocidade de germinação (IVG), comprimento de hipocótilo (CH) e radícula (CR), massa seca de hipocótilo (MSH) e radícula (MSR), curva de embebiçao, além da quantificação da atividade das enzimas antioxidativas, superóxido dismutase (SOD), catalase (CAT), ascorbato peroxidase (APX) e peroxidases totais (POX). O delineamento estatístico utilizado foi o inteiramente casualizado com cinco repetições. Os dados foram submetidos à análise de variância (ANOVA) e as médias obtidas para os tratamentos foram comparadas pelo teste Tukey a 5% de significância. As médias obtidas nos tratamentos com e sem SNP foram comparadas pelo teste F a 5% de significância e os tempos de embebição na análise das atividades das enzimas, também pelo teste Tukey a 5%. Para a realização da curva de embebição realizou-se regressão com ajuste polinomial de grau 3. No segundo experimento, as sementes de gergelim foram semeadas em substrato umedecido com água ou em diferentes concentrações de polietileno glicol (PEG 6000) e PEG 6000 acrescido de SNP nos seguintes tratamentos: 1) água (controle), 2) +200 μM de SNP, 3) 0,1Mpa, 4) -0,1MPa +200 μM de SNP, 5) - 0,2MPa, 6) -0,2MPa +200 μM de SNP, -7) 0,3MPa e 8) -0,3MPa, +200 μM de SNP. As mesmas determinações e análises estatísticas do primeiro experimento foram realizadas. Conclui-se que ambos os estresses causaram a redução da germinação e do vigor, sendo Xi as maiores concentrações as mais prejudiciais às sementes de gergelim. O ON foi capaz de amenizar os danos causados pelos estresses invertendo parcialmente todos os parâmetros fisiológicos avaliados, o que proporcionou maior e mais rápida germinação além de maior desenvolvimento inicial das plântulas. O cádmio e o PEG 6000 provocaram menor absorção e menor ganho de massa nas maiores concentrações em relação à água assim como um prolongamento da Fase II da embebição. Em relação ao sistema antioxidante, houve aumento da atividade enzimática no período de 0 a 24 horas, demonstrando organização do sistema antioxidante nas sementes de gergelim com o decorrer do tempo. Observou-se que nos maiores potenciais e nas soluções mais concentradas, a atividade enzimática foi maior, o que sugere atividade desintoxicante dessas moléculas em sementes submetidas ao déficit hídrico e solução de cádmio mais rigorosa. O uso do SNP, de maneira geral, aumentou a atividade das enzimas antioxidantes, evidenciando um eficiente sistema de eliminação das espécies reativas de oxigênio formadas.
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Tamura, Hitoshi [Verfasser], Peter [Akademischer Betreuer] Schieberle, and Karl-Heinz [Akademischer Betreuer] Engel. "Identification of new key aroma compounds in roasted sesame seeds with emphasis on sulfur components / Hitoshi Tamura. Gutachter: Karl-Heinz Engel. Betreuer: Peter Schieberle." München : Universitätsbibliothek der TU München, 2012. http://d-nb.info/1030100160/34.
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Kareem, Zana [Verfasser]. "Biomedical Applications and Secondary Metabolite Profiling of Hyoscyamus niger and Sesamum indicum Seed, Root and Hairy Root Cultures / Zana Kareem." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1223706249/34.
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Santos, Manoel Galdino dos. "Gergelim irrigado em função da adubação nitrogenada em duas safras agrícolas." Universidade Federal Rural do Semi-Árido, 2017. http://bdtd.ufersa.edu.br:80/tede/handle/tede/767.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico
Sesame is an oleaginous that presents great economic potential, due to the possibilities of exploration. It is a crop of low productivity, and may be superior when management in fertilization is appropriate. However, more information about the nutrition of the crop is needed. Nitrogen is one of the nutrients that most limits the production of sesame because it is responsible for important functions of metabolism and nutrition of the crop. Therefore, the objective of this work was to evaluate the cultivars of irrigated sesame in the function of nitrogen fertilization in two agricultural crops. Experiments were conducted at the Experimental Farm Rafael Fernandes, belonging to the Federal Rural Semi-Arid University, Mossoró-RN, from February to May (1st crop) and July to October (2nd crop) in 2016. The experimental design was in randomized complete blocks with four replications in a subdivided plots scheme, being allocated in the plots five nitrogen doses (0, 30, 60, 90 and 120 kg ha-1), and the four sesame cultivars in the subplots (CNPA G2 , CNPA G3, CNPA G4 and BRS Silk). The variables evaluated were: nitrogen content in the leaf diagnosis, plant height, stem diameter, number of leaves and capsules, dry mass (leaf, stem, capsules and plants), seed productivity, agronomic efficiency, oil and protein content. The dose of 120 kg ha-1 of nitrogen provided the best agronomic performance for sesame. The cultivar CNPA G4 had the highest productive performance. The cultivar BRS Silk had a higher oil content. The second crop provided better agronomic performance to the sesame crop
O gergelim é uma oleaginosa que apresenta grande potencial econômico, devido às possibilidades de exploração. É uma cultura de baixa produtividade, podendo ser superior quando há manejo adequado na adubação. Contudo, maiores informações sobre a nutrição da cultura precisam ser obtidas. O nitrogênio é um dos nutrientes que mais limitam a produção do gergelim, por ser responsável por importantes funções do metabolismo e da nutrição da cultura. Diante disso, o objetivo deste trabalho foi avaliar cultivares de gergelim irrigado em função de adubação nitrogenada em duas safras agrícolas. Os experimentos foram conduzidos na Fazenda Experimental Rafael Fernandes, pertencente à Universidade Federal Rural do Semi-Árido, Mossoró-RN, no período de fevereiro a maio (1ª safra agrícola) e julho a outubro (2ª safra agrícola) de 2016. O delineamento experimental foi em blocos completos casualizados com quatro repetições em esquema de parcelas subdivididas, sendo alocadas nas parcelas cincos doses de nitrogênio (0, 30, 60, 90 e 120 kg ha-1), e nas subparcelas as quatros cultivares de gergelim (CNPA G2, CNPA G3, CNPA G4 e BRS Seda). As variáveis avaliadas foram: teor de nitrogênio na folha diagnóstico, altura de plantas, diâmetro do caule, número de folhas e cápsulas, massa seca (folha, caule, cápsulas e plantas), produtividade de sementes, eficiência agronômica, teor de óleo e proteína. A dose de 120 kg ha-1 de nitrogênio propiciou o melhor desempenho agronômico para o gergelim. A cultivar CNPA G4 teve o maior desempenho produtivo. A cultivar BRS Seda teve maior teor de óleo. A 2ª safra agrícola proporcionou melhor desempenho agronômico à cultura do gergelim
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Rajendran, S. "Studies on the major protein components of Sesame seed (Sesamum Indicum L.)." Thesis, 1990. http://hdl.handle.net/2009/1900.
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Prakash, Karnika. "Studies on sesame seeds and their co-product (White/black) for its functional and nutritional applications." Thesis, 2017. http://localhost:8080/xmlui/handle/12345678/7503.
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Li-Jen, Lin, and 林麗娟. "Steroleosins,Sterol-Binding Dehydrogenase in Sesame Seed Oil Bodies." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/61161095577708835179.
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博士國立中興大學
生物科技學研究所
92
ABSTRACT Seed oil bodies are lipid storage organelles that comprise a triacylglycerol matrix surrounded by a monolayer of phospholipids embedded with proteins. The oil-body associated proteins include the abundant structural protein, oleosin and at least three minor proteins, Sop1-3. Recently, the gene encoding Sop1 protein was cloned from maturing sesame seeds. The Sop1 sequence analyses and biochemical studies indicated that the protein is calcium-binding, and thus named caleosin. This dissertation intended to study structure-function relationship of Sop2 and Sop3 and their targeting to oil bodies during seed maturation. In 2000, the Sop2 cDNA sequence and its corresponding genomic sequence from maturing sesame seeds was cloned by immuno-screening. The deduced protein, tentatively named steroleosin, seems to exist in diverse seed oil bodies, and comprises an oil-body anchoring segment preceding a sterol-binding dehydrogenase involved in signal transduction in diverse organisms. Southern hybridization implies that one steroleosin gene and certain steroleosin-like genes may exist in sesame genome. The results suggest that different sterol-binding dehydrogenases/reductases may be present in diverse plant tissues and involved in signal transduction. In 2002, the Sop3 cDNA sequence was cloned by PCR, the last and the least abundant of the proteins identified in oil bodies of sesame seeds as far. Sequence comparison revealed that Sop2 and Sop3 were homologous proteins, and thus tentatively named steroleosin-A and steroleosin-B. These two steroleosins possessed a conserved NADP+ binding subdomain but a diverse sterol-binding subdomain of different size. Although Sop2 and Sop3 were found homologous, they could not be cross-recognized immunologically with the antibodies prepared in our lab. Dehydrogenase activity detected in their expressed proteins indicated that steroleosin-B might comparably possess a broader sterol selectivity and higher NADP+ specificity than steroleosin-A. In 2003, I proved that steroleosin-A/steroleosin-B and caleosin/oleosin may target to maturing oil bodies via distinct pathways. The hydrophobic domain responsible for oil-body anchoring is located in the N-terminal region of steroleosin, but in the central region of caleosin or oleosin. Steroleosin possessed a free methionine at its N-terminus while caleosin and oleosin were N-terminally blocked. In contrast with caleosin and oleosin, steroleosin preferentially targeted to microsomal membranes instead of artificial oil emulsions in an in vitro competition experiment.
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Kafando, Wendata Achille, and 艾書立. "Sesame Seed Exports in Burkina Faso: Performance and Determinants." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/27u926.
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碩士國立臺灣大學
農業經濟學研究所
107
Following recent tremendous growth in volume and value of sesame seed exports from BFA, and with an unequaled performance observed, we deemed it necessary to identify through a co-integration approach the effects of key determinants and their magnitude on sesame exports performance. Purposely, this thesis specified export earnings of sesame seed as the independent variable to analyze the country export performance using time series data for the period of 47 years (1970-2016). One co-integration vector is observed in the system. Thereby the Vector Error Correction Model (VECM) is performed in order to tie the short-run dynamics to the long-run equilibrium. The empirical results reveal that nominal exchange rate, producer price, world export volume of sesame seed as a proxy of world demand, and world export price are key factors affecting the country’s exports performance in the short-run. Moreover, the empirical results show that nominal exchange rate, the production, producer price and world demand are factors affecting positively exports performance of sesame seed in the long-run. However, the effects of producer price and world demand are insignificant. The long-run results show evidence of a significant and negative effect of international prices on sesame exports performance in the long-run. Both the negative effects and the insignificant effects could be caused by the unaddressed short-run inefficiencies and development constraints in the sector. Identifying and critically addressing these inefficiencies is necessary for the country to keep its exports competiveness in the future. The Error Correction Term (ECT) adjusts any deviation from the long-run to the equilibrium by 36.86% within a year.
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Cai, Ying-Chen, and 蔡映茞. "Studies on chemical and functional properties of sesame flour and proteins prepared from sesame seed cake." Thesis, 1987. http://ndltd.ncl.edu.tw/handle/16179698545599925344.
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Chang, Mei-Ling, and 鄭美玲. "Purification and characterization of cysteine protease inhibitor in sesame seed." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/3v395r.
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碩士元培科學技術學院
生物技術研究所
94
Plant cysteine protease are found particularly in the vacuoles and are responsible for the mobilization of storage proteins during seeds germination. This provides nitrogenous nutrients to support the growth of seedlings. Cystains, the cysteine protease inhibitors, are assumed to regulate endogenous cysteine protease activities during seed maturation and germination when stored reserves are mobilized for embryogenesis and seedling growth. Also they are found to play roles in response to plant defense by inhibiting digestive proteases from insect. Several models of interaction between cystatins and their target cysteine protease are studied, revealing the improtance of three conserved regions of cystatins: glycine residue near the amino-terminal, a central QXVXG motif, and a tryptophan residue near the carboxyl-terminal. Sesame cystatin with molecular mass 22 kDa, an additional extension sequences with unkonwn role is found in sesame cystatin followed three conserved regions. For the study of roles of sesame cystatin in the regulation of endogenous cysteine protease activity, both full-length and N-terminal consreved sequences are cloned and expresse in E.coli. The purified recombinant proteins are coupled as affinity tags to purify the endogenous cysteine proteases in mature and germinated seeds. Furthermore, we have tested the sesame cystatin against to phytopathogenic fungi and bacteria. Four of six purified proteins with molecular masses 30, 34, 45 and 48 kDa form mature seeds and 32, 45 , 48 and 52 kDa from germination seeds, respectively, as determined by SDS-polyacrylamide gel electrophoresis showed protease activities analyzed by the zymographic assay. The sesame cystatin did not exert antibacterial activity, by contrast, the full-length SiCYS, SiCYS-N and SiCYS-C cystatin fully inhibited the growth of two fungi (Trichoderma reesei ， Aspergillus sydowii ).
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Chen, Emily C. F., and 陳靖棻. "Identification of Three Novel Seed Oil Body Proteins in Sesame." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/94237134531967892518.
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Jacob, Jacqueline P. "The feeding value of Kenyan sorghum, sunflower seed cake and sesame seed cake for poultry." Thesis, 1993. http://hdl.handle.net/2429/2109.
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The availability of affordable, quality feed has been a limiting factor in the development of the Kenyan poultry industry. Kenya already has an established feed industry, but feed production has been limited by the availability of suitable feedstuffs. The potential of Kenyan sorghum, sunflower seed cake (SFC) and sesame seedcake (SSC) as feed ingredients in poultry diets was evaluated at the University of Nairobi, Kenya.
A survey of Kenyan sorghum revealed a large variation in the composition of the grains grown. Local sorghum varieties tended to have very high tannin content (up to 13.8% of dry matter) and would most likely be unsuitable for inclusion at high levels in poultry diets. Two of the improved sorghum varieties (Serena and Seredo) had tannin levels of less than 5% and may be suitable energy sources in poultry feeds. Serena was the improved sorghum variety most readily available for purchase in large quantities and was the brown sorghum variety used in all feed trials. A survey of locally produced SFC revealed a large variation in the composition of the cakes available. The main factor limiting the use of SFC in poultry diets is its high fibre content. The crude fibre content of the SFC samples analyzed varied from 24.1 to 40.2% of dry matter. The average composition of the SFC samples, on a dry matter basis, was 29.8% crude protein, 12.5% ether extract, 30.8% crude fibre, 7.2% ash and 19.7% nitrogen-free extract but, because of the wide variation in composition of the cakes, the use of average values for diet formulation could lead to substandard feeds. Locally produced SSC appeared to have less variation in its composition than the locally produced SFC. Excluding one sample which had a very high ether extract and crude fibre content, the average composition of the SSC samples, on a dry matter basis, was 49.8% crude protein, 11.1% ether extract, 9.8% crude fibre, 14.2% ash, and 21.8% nitrogen-free extract. From the results of this study it was concluded that the composition of Kenyan sorghum, SFC and SSC is very different from that of similar products produced in North America or Europe. The use of North American or European tables of composition for diet formulation, therefore, is not recommended. A preliminary study involving two four-week feeding trials investigated the possibility of overcoming any detrimental effects of sorghum tannin on growth or feed efficiency by supplementing the diet with intact protein or with D,L-methionine. In both trials, however, Serena sorghum (tannin content of 2.0-2.2% catechin equivalents) was substituted for white maize with no effect on final body weight or feed conversion ratio (FCR). The feeding values of Kenyan Serena sorghum, SFC and SSC were studied in an eight week broiler trial and a twenty-four week layer trial. Substituting Serena sorghum for white maize in the diets reduced growth for broilers and egg production for layers. The reduction in feed costs associated with the Serena sorghum diets was not sufficient to compensate for the reduction in body weight or egg production so that, based on December 1992prices, it was not economical to substitute Serena sorghum for maize in broiler or layer diets. Serena sorghum would be an economical substitute for white maize in broiler diets if the price of sorghum was reduced 16.7-26.8%.A 6.3-21.6% reduction in the price of sorghum is required to make it an economical substitute for white maize inlayer diets. The level of the price reduction required is dependent upon the main protein source of the diet. Substituting a portion of the imported soybean meal (SBM) in the diet with locally produced SFC had no effect on final body weight for broilers, but resulted in significantly lower egg production for layers. Based on December 1992 prices, the SFC/SBM broiler diets were more economical than the SBM broiler diets. A 300%increase in the price of SFC is required before it would no longer be economical to replace 30% of the imported SBM of broiler diets with local SFC. The reduction in feed costs associated with the SFC/SBM layer diets was not sufficient to compensate for the reduction in egg production. The price of SFC would have to be almost zero before the SFC/SBM maize diets would be economically competitive with the SBM maize diets. Even if SFC was available at no charge, the SFC/SBM sorghum diets would not be economically competitive with the SBM sorghum diets.
Substituting locally produced SSC for imported SBM in the diet resulted in a significantly higher final bodyweight for broilers, but significantly lower egg production for layers. Based on December 1992 prices, SSC was an economical substitute for imported SBM in the broiler, but not the layer, diets. A 240-250% increase in the price of SSC, is required before SSC would no longer be an economical substitute for imported SBM in broiler diets. A slight reduction in the price of SSC or an increase in the price of SBM would make SSC an economical substitute for imported SBM in layer diets.
Based on the results of this study Kenyan Serena sorghum appears to be a suitable substitute for maize in both broiler and layer diets but further research is required to determine the maximum concentration of sorghum tannin that can be included in the diet without adversely affecting production. Locally produced SFC and SSC appear to be suitable substitutes for imported SBM in broiler but not layer diets. Further research is required into the cause of the depressed egg production for the SFC/SBM and SSC layer diets.
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Wang, Ruey-Ching, and 王瑞卿. "Changes of capsule and seed characters during mature stage in sesame." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/39590455217859768178.
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碩士國立中興大學
農藝學系
87
This study is on change of capsule and seed characters during maturation in sesame. The experiment materials including Tainan 1,Tainan white s-2,BL26,BL18,W94,W88 varieties. The results obtained are summarized as follows： Change of capsule dry weight and capsule fresh weight showed no significant difference in the 4-6 nodes from above marker node on 41-44 days after anthesis in sesame. But the BL26 change of capsule dry weight and capsule fresh weight showed no significant difference on 40 days after anthesis in all nodes. The change of 100 seeds weight coincides with capsule fresh weight. When capsule dry weight is at the largest stage that capsule fresh weight is at the smallest stage, therefore the seed is in the optima mature stage. Change of capsule water percentage showed no significant difference in about the 4 nodes from above marker node on 43-44 days after anthesis in sesame. Change of capsule width showed no significant difference in the 4-7 nodes from above marker node on 42-44 days after anthesis, and the same change as in the capsule dry weight. The trend of Capsule length has change no different in most of nodes. Change of capsule chlorophyll content of different node with mature stage showed difference in sesame varieties. And all capsule chlorophyll content are not in trend to stable stage.
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YAOGO, sophie, and 堯素芬. "Sesame Seed Import Demand Analysis in Japan, South Korea and Taiwan." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/31361061495911858983.
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碩士國立臺灣大學
農業經濟學研究所
101
Sesame is an important export crop in Burkina Faso. Annual exports of sesame from Burkina Faso are valued at about US$55 million in 2010 and Burkina Faso is among the suppliers of sesame seed to the world’s largest importer, Japan. These features are poorly recognized and it is timely to develop support and action to facilitate the continued expansion of this sector. The position of sesame is not well understood either in Burkina Faso or in the global trade, and a significantly clearer interpretation of Burkina Faso’s competitive position and opportunities in these asian countries is essential. Therefore, the import demand behavior in these countries is very important in order to make proper recommendations to Burkina Faso government. The compilation of this report has revealed that the import demand in this countries is less responsive to the import price, while it is slightly responsisive to GDP. Based on these results and also a SWOT analysis of the sesame sector in Burkina Faso, the policy recommendations to Burkina Faso government focused on the sector improvement for more competitiveness in these countries where the economy is growing and the import demand is less responsive to price than GDP.
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CIPOLLA, DARIO. "Sesame (Sesamum indicum sp) seed security and breeding programme to improve resilience of small holder farmers in Somalia." Doctoral thesis, 2015. http://hdl.handle.net/2158/990207.
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This work tested on field 5 sesame accessions to identify the most appropriate GAP and to have a phenotypic description of the most relevant morpho–productive traits in different ecosystems. Eventually through the adoption of SSR markers it has been measured the genetic distances among these accessions and 6 more from different African countries in order to design the most appropriate breeding programme per the different ecosystems considered.
A cropping financial analysis was eventually conducted to measure the profitability of the improved crop revealing that Those farmers in riverine on the Shabelle river who adopted the identified GAP (including the seasonal crop rotation with Maize) and best performing varieties increased their income at 1.04 USD/day/person.
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Hong, Hsin-Hui, and 洪心惠. "Molecular cloning and expression of asparaginyl endopeptidase, a specific protease in maturing sesame seed." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/66284419250506411711.
Full textAbstract:
碩士國立中興大學
農業生物科技學研究所
87
There is increasing interest in using crop plants as bioreactors in the past decade including production of modified starches, plastics, industrial and pharmaceutical proteins such as enzymes, antibodies, and antigens. Oleosin, a unique structural protein in plant seed oil bodies, has been designed to carry foreign proteins for expression in seeds. The low density of oil bodies provides an easy method (simply done by subsequent centrifugation) to purify recombinant proteins carried by oleosins. However, the bottleneck for application of this expression system is the high cost of using a specific protease to cleave target proteins from the carrier, oleosin. This study aims to search for a specific protease in seeds to overcome the above obstacle. Seed storage proteins are disulfide-bonded and specifically cleaved into two subunits by a protease termed asparaginyl endopeptidase before their deposit into protein bodies. Using the cDNA library constructed with maturing sesame seeds, the corresponding gene of asparaginyl endopeptidase has cloned by PCR and completely sequenced. Currently, I am trying to over-express this gene in E. coli using the T7 polymerase-promoter system, to purify the expressed protein for antibody induction, and to inspect the adequate condition for the specific protease digestion of the expressed asparaginyl endopeptidase.
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Kusuma, Ramadhani Mahendra, and 馬漢德. "Study on the Interaction of a Cysteine Protease and Its Inhibitor from Sesame Seed." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/6rtxwh.
Full textAbstract:
碩士國立屏東科技大學
生物科技系所
106
Papain-like cysteine protease has been widely used worldwide and has been known to have a specific relationship with cystatin. To study the interactions between cysteine protease and cystatin, soluble fraction of recombinant cysteine protease expressed in Escherichia coli cloned from germinating sesame seed (SiCP) was purified using immobilized-cystatins coupled to sepharose affinity column. Purified-SiCP demonstrates a very similar proteolytic activity compared with papain toward substrate and decisively inhibited by SiCYS. SiCP enzymatic activity was found to be optimum at a slightly acidic pH and stable throughout a wide range of temperature ranged from 30 to 70 oC.
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Huang, Min-Shuan, and 黃閔鉉. "Analysis of Genes Expressed During Germination and Those Related to Lipid Metabolism from Sesame Seed." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/12069899158962797204.
Full textAbstract:
碩士國立屏東科技大學
生物科技系所
101
Sesame (Sesamun indicum L.), an edible oil crops that is versatile with oil content up to 45~60%, is an annual herb belongs to family Pedaliaceae. The oils are majorly triacylglycerols (TAGs) stored in a specialized organelle - oil bodies in seeds, and are the resources of energy and nutrients for seed germination. The oil bodies are degraded during seed germination, and the TAGs are released and decomposed into glycerols and free fatty acids by the action of lipases. Each time through β-oxidation the fatty acids are decomposed to release two carbons of acetyl-CoA which is associated with the glyoxylate cycle (2 acetyl-CoA + 2 NAD+ + FAD → oxaloacetic acid + 2 CoA-SH + 2 NADH + FADH2 + 2H+). Both malate and oxaloacetate can be converted into phosphoenolpyruvate for the production of glucose in gluconeogenesis. The glucose then might enter electron transport chain and citric acid cycle (TCA) to release energy by the formation of ATP supplied for seed germination, and might provide carbon skeleton for cell division. Up to 50% of sesame seed oil is utilized two days after imbibition, it is speculated that lipases with high activity are synthesized before this period. Three partial cDNA fragments of lipases are cloned by PCR cloning with the use of degenerate primers designed based on the conserved sequences of lipases from sesame cDNA library constructed from the early period of seed germination. Specific primers are designed for the cloning of full-length cDNAs for functional expression and characterization. The investigations of this study on actions of different lipases involved in lipid metabolism from sesame would be the important basis for understanding their physiological roles in oil crops and for industrial applications.
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Shi, Ying-ru, and 施盈如. "The Structure and Development of the Sesame Seed Studied with Microscopic Magnetic Resonance Imaging with Various Weighting Approaches." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/48056909855415842514.
Full textAbstract:
碩士國立中山大學
化學系研究所
94
Microscopic magnetic resonance imaging (μMRI) is a powerful tool for studying the structure and dynamics of various biological systems in vivo. A particular advantage ofμMRI related to this type of applications is that many different images can be acquired on the same object, each of them being important and meaningful. In this work, we report the results of applyingμMRI to the study of the biological process of a real plant (sesame seed). With weighting of longitudinal and transverse relaxation, diffusion as well as multi-quantum coherences, a series of images of sesame seed in the process of germination, with spatial resolution of several micrometers or tens of micrometers, has been obtained (examples given in Fig.1). The images are analyzed based on the physiological characteristics of the sesame seed. Some insights are drawn from these images obtained with different weightings.
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Yang, Wei-yuan, and 楊威源. "Investigation of Germination of Sesame Seed with Magic-Angle-Spinning Solid State Nuclear Magnetic Resonance Spectroscopy and Micro-Imaging." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/8d4sbs.
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CHIANG, ZHENG-HE, and 姜政合. "Effect of Adding Soybean, Sunflower Seed, Cottonseed or Sesame Oil on Volatile Components and Fatty Acid Compositions of Peanut Oil." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/6vxkrv.
Full textAbstract:
碩士大仁科技大學
食品科技研究所
106
Although peanut oil (PO) widely consumed has a high nutrition and unique aroma, few manufacturers may mix a low-quality edible oil or even blend some flavors with pure PO in order to make a better profit. Each food lipid composes of its specific fatty acid profile, thus analyzing the compositions of fatty acids is often used to judge its purity or adulteration. Yet this method may not easy to identify its accurate purity or adulterate if the PO is mixed with a small amount of low-quality food oil. PO has a unique flavor, so the analysis combining fatty acid compositions and volatile compounds should have better accuracy. Thus this research was to investigate the fatty acids and volatile components of PO mixed with 0-40% soybean, sunflower seed, cottonseed and 0-10% sesame oils, and furthermore to apply this technique for judging the adulteration of commercial peanut oil products purchased from food market in Taiwan. The results indicated that the ratio of oleic acid/linoleic acid (OA/LA) exceeded a normal range (0.93 - 1.16) as the PO is mixed with 20 % soybean oil, 20% cottonseed oil or 25% sunflower seed oil. Because soybean, sunflower seed, and cottonseed oils had low aroma, the volatile compounds of blended PO mixed with these oils significantly decreased. However, it showed no significant difference in the ratio of 2,5-dimethyl pyrazine/methyl pyrazine (2,5-DMP/MP). As PO mixed with 1-10 % sesame oil, OA/LA ratio of the mixtures remained in a normal range. However, the 2,5-DMP/MP ratio gradually decreased by increasing the amount of sesame oil. When sesame oil was added over 5%, the 2,5-DMP/MP ratio showed lower than 1, which didn't meet reasonable range of pure peanut oil. Compared to PO, PO mixed with 20 % soybean oil, 30% sunflower seed oil, 20% cottonseed oil or 5% sesame oil had similar aroma in consumers’ sensory evaluation. From the analysis of fatty acid composition, it was difficult to identify the difference in the OA/LA ratio between pure PO and PO containing 30% soybean oil plus 5-10% sesame oil. However, the 2,5-DMP/MP ratio of blended PO with >5% sesame oil was obviously lower than that of PO. Two out of five samples from commercial peanut oil products had low OA/LA ratio (< 0.93), and four samples had low 2,5-DMP/MP ratio (< 1). As a consequence, only 20% commercial peanut oil products complied with the standard of pure peanut oil as examining by both OA/LA and 2,5-DMP/MP ratios.
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Sacco, Sandra M. "The effect of sesame seed, tamoxifen, and their combination on tumor growth and bone in ovariectomized athymic mice with estrogen-responsive breast cancer." 2006. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=450618&T=F.
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Ho, Ya-Lan, and 何雅嵐. "Investigation on the antioxidation activities of the embryo of Nelumbo nucifera Gaertn. seed and sesame meal and the effects on prolonging the lifespan of Caenorhabditis elegans." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/63608003454690120654.
Full textAbstract:
碩士國立臺灣大學
食品科技研究所
95
Aging is an inherently complex process that is manifested within an organism at molecular, cellular, organ, and system levels. There are many theories that have been put forward to explain the process of aging, and one mechanism involving free radical damage seems to emerge in a quite consensual manner. Reactive oxygen species (ROS), produced by a variety of processes, can cause extensive oxidative damages, which would be responsible for age-associated loss of functional capacity. Dietary interventions with antioxidants seem to demonstrate some potential benefits on the average life expectancy, probably by reducing the onset of age-related diseases. In the previous study conducted in our laboratory, using “inhibition of aggregated Aβ-induced PC-12 cell death” as the model for Alzheimer’s disease, has shown that embryo of Nelumbo nucifera Gaertn. seed and defatted Sesamum indicum L. have good anti-Alzheimer’s disease capacity. Since both aging and neurodegenerative disease such as Alzheimer’s disease are associated with in vivo oxidative damage, we are therefore interested in investigating the in vitro antioxidation and in vivo anti-aging effects of embryo of Nelumbo nucifera Gaertn. seed and defatted Sesamum indicum L. The crude extracts from different solvents were prepared by extracting these two raw materials with boiling water and four solvents (95% ethanol, acetone, ethyl acetate and n-hexane) at room temperature. Then the different solvent extracts of embryo of Nelumbo nucifera Gaertn. seed and defatted Sesamum indicum L. were tested for two antioxidative assays, including DPPH free radical scavenging effect and Trolox equivalent antioxidant capacity (TEAC) assay. They were also examined for anti-aging activity using the “prolongation of C. elegans lifespan” as the model, and EUK-8 as the positive control. The results of antioxidant assays revealed that the best effects was exhibited by the 95% ethanol extract of the embryo of Nelumbo nucifera Gaertn. seed, followed by its water, acetone, ethyl acetate and n-hexane extract. The EC50 value of embryo of Nelumbo nucifera Gaertn. seed 95% ethanol extract in scavenging DPPH free radicals was 47.32±1.20 μg/mL, while its TEAC value was 0.31 mM Trolox equivalent. The EC50 of the positive control (α-tocopherol) is 8.40±0.13 μg/mL for DPPH scavenging ability and ascorbic acid for TEAC assay (TEAC value was 1.16 mM Trolox equivalent). Different solvents extracts of the defatted Sesamum indicum L. did not show good antioxidant activity from both antioxidant assays. Results of anti-aging model experiment showed that treatment of worms with embryo of Nelumbo nucifera Gaertn. seed 95% ethanol extract had the best effect on extension the lifespan of C. elegans. Treatment of worms with 1000 μg/mL embryo of Nelumbo nucifera Gaertn. seed 95% ethanol extract resulted in an increase in mean lifespan of 25.8% (p＜ 0.001) and also extended maximum lifespan (35 days) and 80% death time (28.2 days). The effect was similar to that of 100 μM (35.5 μg/mL) EUK-8 which can increase mean lifespan by 24.1% (p＜0.001) and can also extend maximum lifespan (35 days) and 80% death time (27.0 days). After liquid-liquid partition of the 95% ethanol extract of the embryo of Nelumbo nucifera Gaertn. seed with n-hexane, ethyl ether, ethyl acetate and water consecutively, the water fraction showed the best antioxidant activity. The EC50 value of the water fraction in scavenging DPPH free radical was 25.17±0.19 μg/mL, and the result of TEAC assay (0.41 mM Trolox equivalent) was also the highest. Among the four fractions, water fraction of the embryo of Nelumbo nucifera Gaertn. seed 95% ethanol extract had the best effect on the lifespan of C. elegans. Treatment of worms with 100 μg/mL water fraction increased their mean (16.3% (p=0.0036)) and maximum (17.2%) lifespan in addition to 80% death time (12.5%). Extension of the lifespan of C. elegans also exhibited dose response was observed. The effect of 100 μg/mL water fraction had similar effect as the 25 μM (8.9 μg/mL) EUK-8 which increased mean lifespan by 16.3% (p=0.0044) and also extended maximum lifespan (13.8%) and 80% death time (14.3%). This study showed that the water fractions of the 95% ethanol extract of the embryo of Nelumbo nucifera Gaertn. seed contained components with antioxidant activity and could also prolong the lifespan of C. elegans. It thus has good potential to be developed as a functional food for anti-aging.
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Sahu, Ranjit Kumar. "Studies on the Structural stability of Alpha Globulin the major protein fractional of sesame (sesamum indicum L.) seeds in specific solvent systems." Thesis, 2002. http://hdl.handle.net/2009/2736.
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Chih-Yu, Chien, and 簡志宇. "Using Supercritical Carbon Dioxide to Extract the Functional Components from Sesame Seeds." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/76196572592266424215.
Full textAbstract:
碩士中州科技大學
保健食品系
101
Sesame indicium(Sesamun L.)is Taiwan's traditional crops, oil crops is also one of the ancient crops, ancient times it was known as the longevity of senior health food, by modern nutritional analysis from now, sesame contains the human body needs a variety of nutrients, including vitamins A, D, E, and rich in B-complex vitamins, and rich in unsaturated fatty acids, sesamin and oleic acid, physical health has great effect. Chinese pharmacopoeia of medicinal functions for sesame that call blindly tonic, there is blood, intestines, fluid, through milk, hair care, nourishing the five internal organs and other effects. For frail and cold hands and feet.Sesame oil(sesameoil),the sesame seeds indicium(Sesamun L.)seeds are derived, which containslignanshave considerable physiological activity included in the human body can be excluded with fatty liver the role of oxygen, improve immune function, anti-tumor, scavenging free radicals, inhibiting lipid peroxidation, anti-colon cancer,lower blood pressure , antithrombotic, cholesterol. Sesame the main lignans compounds sesamin、sesamolin、sesamol、sesaminol and sesamolinol .Traditional ingredients sesame lignans extraction method is to use a solvent extraction method or a cold method, but poor extraction efficiency, heat damage to the heat-sensitive ingredients and the issue of residual organic solvents. In this study, the use of supercritical carbon dioxide extraction technology compounds extracted lignans and set extraction conditions of temperature (40 ℃, 50 ℃, 60 ℃), pressure (10 MPa, 20 MPa, 30 MPa), time (20mins, 30 mins, 40 mins ), auxiliary solvent consumption of alcohol (1.5ml, 3ml, 4.5ml), to achieve the best extraction parameters are temperature 40 ° C (A1), pressure 30MPa (B3), dynamic extraction time 40 minutes (C3) and the auxiliary solvent dosage 4.5ml (D3).The sesame seeds used in this study are commercially available TN1 kinds of extracts obtained compared with the commercially available oils found TN1 sesame seed extract sesamin, sesamol and sesamolin contents were 49.76mg / g, 2.02mg / g and 13.87mg / g, were higher than the commercially available six kinds of sesame oil products sesamin (5.96mg/g-2.73mg/g), sesamol (0.86mg/g-0.08mg/g) and sesamolin (4.50mg / g - 0.41mg / g) content. Therefore, the use of supercritical carbon dioxide extraction technology to replace conventional extraction methods sesame active ingredient can be developed and issued by high purity solvent residue of the active ingredient Sesame health food.
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Ke, Min-Shing, and 柯閔翔. "Construction of cDNA Libraries from Germinating Sesame Seeds and Cloning of Lipase Genes." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/59149066548621481243.
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碩士國立屏東科技大學
生物科技研究所
98
Lipase (triacylglycerol acylhydrolase; EC 3. 1. 1. 3), an enzyme which catalyzes the hydrolysis of triacylglycerol to glycerol and fatty acids, has been widely used in fat, food ingredients, detergents and biotechnology industries. Sesame（Sesamun indicum L.）, belongs to the Pedaliaceae family, is one of most important oilseed crops in the world. Sesame seed possesses more than 50% oil content in dry weight. The storage triacylglycerols (TAGs) is used as energy source for germination and subsequent post-germinative growth. Because more than 50% of oil in sesame seed is exhausted after sprouting for two days, it is suggested that the lipase exhibits highest activity during this stage. The purpose of this study is to construct cDNA libraries from germinating sesame seeds and to clone the lipase genes for functional analysis of gene expression. Sesame seed extracts from different time points after imbibition are subjected for assaying lipase activity. It is found that lipase activity increases after imbibition and the maximum activity is detected at 16 hours after imbibition. Detection of lipase gene expression and cloning of partial cDNAs at different time points are conducted by PCR cloning by the use of designed plant lipase degenerate primers. Three partial lipase/esterase cDNA sequences are cloned, and the result of sequence alignment shows that they may be different lipase/esterase genes. Therefore, cDNA libraries of 8 and 12 hours after imbibition are constructed and used to clone full-length lipase/esterase cDNA sequences. Expression analysis of three lipase genes could be further studied.
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Tai, Sorgan S. K., and 戴守谷. "Expression Pattern and Deposition of Protein Body and Oil Body Proteins in Developing Sesame Seeds." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/81028716411971514497.
Full textAbstract:
博士國立中興大學
農業生物科技學研究所
89
Plant seeds store protein, starch, and oil as amino acid and energy sources for germinating and post-germinative growth. In sesame seeds, two major reserved organelles are protein body and oil body, which comprise 15-25% protein and 45-60% oil of seed dry weight, respectively. Protein bodies are 0.1-25 mm in diameter and deposited of storage proteins during seed development stage. To examine whether the abundant 11S globulin and/or 2S albumin account for the high quality of nutritious content in sesame, the corresponding cDNA sequences of two 11S globulin and two 2S albumin precursors were cloned. Deduced amino acid sequences indicate that one of the 2S albumin is a sulfur-rich protein. In addition, a full-length cDNA encoding a putative 7S globulin as a minor storage protein in sesame was confirmed by immunodetection. Both Northern and Western analyses suggest that storage proteins are expressed and deposited to protein bodies later than the initiation of oil body formation in accordance with the observation in electron microscopy. Immunogold labeling reveals that all the three storage proteins are co-existent in each protein body of sesame seeds, and that 2S albumin and 7S globulin are preferentially located in the peripheral portion of protein bodies. Oil bodies are 0.5-2.5 mm in diameter and contain a TAG matrix surrounded by a monolayer of phospholipids (PLs) embedded with structural proteins, oleosins, and some minor proteins of higher molecular mass. In order to investigate the oleosin isoforms in sesame, two full-length cDNA clones were sequenced and deduced to encode sesame oleosin isoforms of 17 kDa and 15 kDa oleosin, respectively. During seed development, 17 kDa oleosin emerged later than 15 kDa oleosin, but represented the most abundant protein in mature oil bodies. Artificial oil bodies were reconstituted with triacylglycerol, phospholipid, and sesame oleosin isoforms. The results indicated that while reconstituted oil bodies could be stabilized by both oleosin isoforms, 15 kDa olesin provided a better structural stability than 17 kDa oleosin. Expression pattern and deposition of storage proteins and oleosins revealed that their corresponding promoters are expressed at various levels and regulated temporally. The results may provide a useful clue for the investigation of gene regulation and potential application of sesame seeds as a system for foreign protein expression and purification.
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Melo, Diana Isabel Lopes de. "Chia, flax, poppy and sesame seeds: valorisation of by-products from oil extraction and label compliance." Master's thesis, 2019. https://hdl.handle.net/10216/121565.
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Chih-Yu, Chan, and 詹智宇. "Effects of Different Drying Temperatures, Drying Days and Cutting Treatments on the Compositions of Sesame Seeds." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/04710367574341735242.
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碩士國立嘉義大學
農藝學系研究所
97
This study aimed to investigate (1) the best drying temperature and drying days and (2) the changes of the compositions and fatty acid of sesame seeds under different treatments. By reducing the damages caused during the rainy seasons or typhoons, the benefits of the farmers were thus to be increased. These sesame seeds, TN1, were planted in the field as spring and fall crops in 2007. Five different drying temperatures, five different drying days and three different cutting treatments were conducted to investigate the agronomic characters, compositions and fatty acid of sesame seeds after harvested. Results were summarized as follows: As for the character of ratio of mature seeds no., ratio of mature seeds wt., and 1000 seeds wt., T5D10C1 (45℃, 10 drying days and non-cutting treatments) was found to be the best treatment. As for the compositions of sesame seeds, T4~5D8~10 and T5D8~10 (40℃or 45℃and 8~10 drying days) gained the highest percentage of crude protein and fat. If the space of drying machine and delivering belt were limited, half-cutting sesame plants were able to reserve higher crude protein and fat percentages. As for the compositions of fatty acid, the treatment of T2D2~10 (30℃ and 2~10 drying days) gained higher percentage of oleic acid (C18:1), linolenic acid (C18:3), palmitic acid (C16:0). However, T5D2~10 (45℃ and 2~10 drying days) was found to gain higher percentages of stearic acid (C18:0), linoleic acid (C18:2) and arachidic acid (C20:0).
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Melo, Diana Isabel Lopes de. "Chia, flax, poppy and sesame seeds: valorisation of by-products from oil extraction and label compliance." Dissertação, 2019. https://hdl.handle.net/10216/121565.
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Peng, chi-chung, and 彭及忠. "Ontogeny of oil bodies Monitored with the accumulation of their three essential constituents in developing Sesame Seeds." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/19565447190997884907.
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碩士國立中興大學
農業生物科技學研究所
85
ABSTRACTPlant seeds store triacylglycerols in discrete organelles called oil bodies. An oil body is surrounded by a half-unit membrane of phospholipids and alkaline proteins termed oleosins. Triacylglycerols and two oleosin isoforms of 17 and 15 kDa were exclusively accumulated in oil bodies of developing sesame seeds. During seed development, oleosin 17 kDa emerged later than oleosin 15 kDa, yet developed subsequently into the most abundant protein in mature oil bodies. Phosphotidylcholine, the major phospholipid in oil bodies, was amassed in ER during the formation of oil bodies. The results support that oil bodies originate from ER with actively synthesized triacylglycerols and phosphotidylcholine whereas oleosins target onto budding oil bodies directly without packaging in ER. Prior to the formation of oil bodies, few oil droplets of smaller size were observed in vivo and in vitro. These oil droplets were unstable presumably due to the lack of steric hindrance shielded by oleosins. The temporary maintenance of these droplets as small entities seemed to be provided by phospholipids wrapped in ER. Oil bodies assembled in late developing stages possessed a higher ratio of oleosin 17 kDa over oleosin 15 kDa and were utilized earlier during germination. It seems that the proportion of oleosin 17 kDa on the surface of oil bodies is related to the priority of their utilization.
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Hsiang-Chun, Hsieh, and 謝香君. "Effects of Different Drying Temperatures, Drying Days and Cutting Treatments on the Antioxidant abilities of Sesame Seeds." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/59202410606586890249.
Full textAbstract:
碩士國立嘉義大學
農藝學系研究所
99
This study aimed to investigate (1) the best drying temperature and drying days and (2) the changes of the antioxidant abilities and antioxidant contents of sesame seeds under different treatments. By reducing the damages caused during the rainy seasons or typhoons, the benefits of the farmers were thus to be increased. These sesame seeds, TN1, were planted in the field as spring and fall crops in 2008. Five different drying temperatures, five different drying days and three different cutting treatments were conducted to investigate the agronomic characters, antioxidant abilities and antioxidant contents of sesame seeds after harvested. Results were summarized as follows: As for the character of ratio of mature seeds no., ratio of mature seeds wt., and 1000 seeds wt., T3D10C1 (35℃, 10 drying days and non-cutting treatments) was found to be the best treatment. Results of this study indicated, among five drying temperature treatments, temperature 35℃ had the best effect on DPPH• scavenging activity of the fall crops. Among different drying day treatments, day duration between 10~14 days had the best effect on the antioxidant ability (Both DPPH and ABTS scavenging activity) of the spring crops. The effect on antioxidant ability showed no significant difference among those three different cutting treatments of the spring and fall crops. As for the contents of antioxidant precursors, the treatment of D10 (10 drying days) gained higher sesamin and sesamolin contents.
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Hsiao, Shih-Liang, and 蕭世良. "Determination of Storage Proteins in Sesame Seeds and Glycosylation of Pectin Methylesterase in Jelly Fig Achenes by Mass Spectrometry." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/82117130931017341371.
Full textAbstract:
博士國立中興大學
生物科技學研究所
97
Mass spectrometry has been nowadays extensively used in protein research, including protein identification, peptide sequencing and post-translational modification such as phosphorylation, glycosylation and disulfide bond formation. The present study consists of two parts, which were both mainly achieved by mass spectrometry. In the first part, the relative positions of major sesame seed storage proteins, 11S globulin and 2S albumin, were demarcated in SDS-PAGE. These proteins are potential allergens, and the designation thereof could be beneficial for the diagnosis of allergens in pertinent food products. In the second part, the glycosylation site and glycan structures of pectin methylesterase in jelly fig achenes are determined to provide a better understanding on the relation between glycosylation and enzymatic activity. In the first part, two gene families separately encoding four 11S globulin (two were newly found; two were formerly published) and three 2S albumin (one was newly found; two were formerly published) were identified from a database search of 3328 expressed sequence tag (EST) sequences. The full-length cDNA sequences of the new isoforms had been completed in our laboratory. In the present work, the four 11S globulin and three 2S albumin isoforms resolved in 12.5% and 18% polyacrylamide gels were confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the second part, pectin methylesterase (PME) in jelly fig achenes has been detected as a glycoprotein previously. In the present work, the glycosylation site was identified to be at Asn153 of mature jelly fig PME by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). The major N-glycans released from native PME were identified as xylosylated, non-core fucosylated, pauci-mannose and complex-type structures by tandem mass spectrometry (MS/MS) analyses. Molecular modeling of jelly fig PME indicated that the N-glycan was putatively attached to the back region of the active site.
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Chuang, Wei-gang, and 莊維綱. "Liquid State Nuclear Magnetic Resonance Spectroscopic Investigation of Phosphorus Metabolism during Germination of Sesamum indicum Seed." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/72508814757878020674.
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Kareem, Zana. "Biomedical Applications and Secondary Metabolite Profiling of Hyoscyamus niger and Sesamum indicum Seed, Root and Hairy Root Cultures." Doctoral thesis, 2020. http://hdl.handle.net/21.11130/00-1735-0000-0005-1520-F.
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Romano, Ana Rita Jorge. "Metals in Food Products with Rising Consumption : Nutritional and Toxicological Evaluation." Master's thesis, 2014. http://hdl.handle.net/10451/26890.
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Ye, Guan-Hong, and 葉冠宏. "Secondary Metabolites from The Oil Bodies of Sesamum indicum, Camellia oleifera and C. brevistyla, and from The Seed Coats of C. oleifera." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/s3wvub.
Full textAbstract:
碩士國立虎尾科技大學
生物科技研究所
103
The sesame oil and tea oil are appreciated as healthy oil in Taiwan, mainly due to the potential antioxidant activity of sesame oil and high content of polyunsaturated fatty acids in tea oil. However, the secondary metabolites from the oil bodies of these species were not comprehensively studied. Therefore, in the present study it was aimed to purify and identify the secondary metabolites in the oil bodies of seeds of Sesamum indicum, Camellia oleifera, and C. brevistyla. With the assistance of these results, it could construct the whole blueprint regarding the chemical constituents and physiological mechanism. It is also hopeful to elucidate the relationship between the compounds and signal transduction. Moreover, it could provide the bioactivity data of these principles. Totally, fifteen, eight, and four compounds were identified from the methanol extracts of oil bodies of S. indicum, C. oleifera, and C. brevistyla, respectively. In addition, seven compounds were isolated from the water layer of methanol extracts of kernels of C. oleifera. Some of these identified compounds were reported to exhibit antioxidant and anti-inflammatory bioactivity. These results indicated the high potential of sesame oil and tea oil as healthy food in the future.
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Lin, Mei-Chi, and 林美琪. "1.Constituents from the Root Bark and Stem Bark ofMoringa oleifera2.Constituents from the Seeds of Sesamum indicumand their Antioxidant Activity3.Bioactive Constituents from the Chinese Medicine Extracts and their Inhibiting Activity Against Colletot." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/8bjk4v.
Full textAbstract:
碩士國立虎尾科技大學
生物科技研究所
99
In the preliminary cytotoxic bioassay, the chloroform layer of methanol extracts of M. oleifera demonstrated the significant cytotoxicity toward the doxorubicin resistant human breast adenocarcinoma cell lines (MCF-7/ADR) and rat pulmonary fibrosis cells (MLF). Therefore, the present research is aimed to purify and identify the constituents of M. oleifera. The methanol extracts of root barks and stem barks of M. oleifera were fractionated and further purified to afford 27 and 11 compounds, respectively. Among the isolates two compounds was reported from the natural sources for the first time. These isolates would be subjected into the biological activity examinations to explore new natural anticancer drugs. The methanol extract of sesame (Sesamum indicum) seeds was fractionated and purified to afford 30 compounds. Among these isolates two were isolated from the natural sources for the first time. To evaluate their in vitro antioxidant potentials, the purified lignans and their derivative prepared by semi-synthesis were examined for the scavenging activities of DPPH free radicals and superoxide anions. Moreover, the capability of chelating ferrous ions and reducing power of these sesame lignans were also tested. The results suggest that, besides the well-known sesamolin and sesamin, the minor sesame lignans (+)-pinoresinol, (–)-piperitol and (+)-(7S,8''R,8R)-acuminatolide are also adequate active ingredients and may be potential sources for the development of healthy foods. In the organic vegetables cultivation fields, the cabbage anthracnose caused by Colletotrichum higginsianum was a serious problem. Thus in the present study, five different traditional Chinese medicines, including Galla rhois, Eugenia caryophyllata Thunb., Cinnamomum cassia, Polygonum cuspidatum Sieb. et Zucc., and Rheum officinale Baillon were extracted and purified. The contents of indicator compounds in the crude extracts and fractions were analyzed with the aid of high-performance liquid chromatography. Furthermore, the antifungal activities of the crude extracts, fractions, and indicator compounds were examined to explore the active principles against C. higginsianum. From our research results, it is hopeful to discover new botanical pesticides derived from the plant sources.