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Journal articles on the topic 'Seven-day chicken embryo'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Khokhlov, R. Y., and S. I. Kuznetsov. "Morphogenesis of the ovary of a chicken embryo in the germinal period of embryonic ontogenesis." International Journal of Veterinary Medicine, no. 4 (February 2, 2023): 353–56. http://dx.doi.org/10.52419/issn2072-2419.2022.4.353.

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The article is devoted to the morphogenesis of the ovary of chickens in embryonic ontogenesis, namely, in the embryonic period, which lasts from the beginning of incubation to the 8-day age of the chicken embryo. The aim of the study was to study the morphology of the development of the ovary of chickens in early embryonic ontogenesis. The objectives of the study included: determining the timing of the onset of ovarian development in chicken embryos; determining the age of the chicken embryo in which the reduction of one of the gonads begins, namely the right one; determining the size of epith

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2

Putra, Raditya Pradana, Sri Murtini, and Okti Nadia Poetri. "Gambaran Patologi Embrio Ayam yang Terinfeksi Avian orthoreovirus Isolat Lokal." JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI 9, no. 1 (2024): 68. http://dx.doi.org/10.36722/sst.v9i1.2143.

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<p><strong><em>Avian orthoreovirus</em></strong><strong> (ARV) is widespread and found in almost every commercial poultry farm and other poultry species. Virus isolation from local isolates is necessary to obtain vaccine seeds that are homologous to viruses in the field. The R&amp;D Laboratory of PT Vaksindo Satwa Nusantara successfully isolated ARV from field cases. Inoculation of SPF Embryonated Chicken Eggs (ECE) is the protocol used to evaluate virus titer, especially to calculate the antigen titer of vaccine prototype candidates. The gross pathology

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Huang, Lvwen, Along He, Mengqun Zhai, Yuxi Wang, Ruige Bai, and Xiaolin Nie. "A Multi-Feature Fusion Based on Transfer Learning for Chicken Embryo Eggs Classification." Symmetry 11, no. 5 (2019): 606. http://dx.doi.org/10.3390/sym11050606.

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The fertility detection of Specific Pathogen Free (SPF) chicken embryo eggs in vaccine preparation is a challenging task due to the high similarity among six kinds of hatching embryos (weak, hemolytic, crack, infected, infertile, and fertile). This paper firstly analyzes two classification difficulties of feature similarity with subtle variations on six kinds of five- to seven-day embryos, and proposes a novel multi-feature fusion based on Deep Convolutional Neural Network (DCNN) architecture in a small dataset. To avoid overfitting, data augmentation is employed to generate enough training im

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Hansen, Cristina M., Brandt W. Meixell, Caroline Van Hemert, Rebekah F. Hare, and Karsten Hueffer. "Microbial Infections Are Associated with Embryo Mortality in Arctic-Nesting Geese." Applied and Environmental Microbiology 81, no. 16 (2015): 5583–92. http://dx.doi.org/10.1128/aem.00706-15.

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ABSTRACTTo address the role of bacterial infection in hatching failure of wild geese, we monitored embryo development in a breeding population of Greater white-fronted geese (Anser albifrons) on the Arctic Coastal Plain of Alaska. During 2013, we observed mortality of normally developing embryos and collected 36 addled eggs for analysis. We also collected 17 infertile eggs for comparison. Using standard culture methods and gene sequencing to identify bacteria within collected eggs, we identified a potentially novel species ofNeisseriain 33 eggs,Macrococcus caseolyticusin 6 eggs, andStreptococc

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5

Kencana, Gusti Ayu Yuniati, I. Nyoman Suartha, I. Made Kardena, and Arini Nurhandayani. "ISOLATION AND CHARACTERIZATION OF AVIAN INFLUENZA VIRUS H5N1 SUBTYPE FIELD ISOLATES FROM BALI FOR VACCINE CANDIDATES." Jurnal Veteriner 21, no. 4 (2020): 530–38. http://dx.doi.org/10.19087/jveteriner.2020.21.4.530.

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A research on the isolation and characterization of the Avian Influenza H5N1 subtype field isolate has been carried out at the BSL-3 Laboratory of PT Sanbio Laboratories, Bogor. The aim of the study was to prepare a candidate for the H5N1 subtype Avian Influenza virus vaccine. Virus isolates were taken from field isolates from Bali. A total of seven field H5N1 AI subtypes from Bali were characterized in Bogor. The isolates were: isolate 3A, isolate 4A, isolate 9C, isolate 10 A, isolate 10 C, isolate P65, isolate P67. The passage of isolates was carried out on 9-day-old embryonic Specific Patho

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6

Shah, Abid Ullah, Zhisheng Wang, Yating Zheng, et al. "Construction of a Novel Infectious Clone of Recombinant Herpesvirus of Turkey Fc-126 Expressing VP2 of IBDV." Vaccines 10, no. 9 (2022): 1391. http://dx.doi.org/10.3390/vaccines10091391.

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The increased virulence of infectious bursal disease virus (IBDV) is a threat to the chicken industry. The construction of novel herpesvirus of turkey-vectored (HVT) vaccines expressing VP2 of virulent IBDV may be a promising vaccine candidate for controlling this serious disease in chickens. We generated a novel infectious clone of HVT Fc-126 by inserting mini-F sequences in lieu of the glycoprotein C (gC) gene. Based on this bacterial artificial chromosome (BAC), a VP2 expression cassette containing the pMCMV IE promoter and a VP2 sequence from the virulent IBDV NJ09 strain was inserted into

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Herlina, Pratiwi, Haskito AEP, Setianingrum Ani, et al. "Evaluating Teratogenic Effects: Examination of the Impact of TCDD on Craniofacial Characteristics and Body Measurements of Isa Brown Chicken Embryos Under Controlled Experimental Conditions." GPH-International Journal of Agriculture and Research 07, no. 01 (2024): 01–07. https://doi.org/10.5281/zenodo.10453678.

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<strong>2,3,7,8-tetrachlorodibenzo-p-dioxin, sometimes referred to as TCDD is a chemical molecule that is generated via the process of burning. It poses a significant threat to both people and animals due to its toxic effects. The potential adverse effects include dermatological injury, immunosuppression, hepatotoxicity, increased risk of cancer, harm to the developing fetus, reproductive disorders, disruption of development processes, neurobehavioral impairments, and particular endocrine disturbances. Furthermore, this chemical has the capacity to influence the process of embryonic developmen

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8

Stedman, N. L., T. P. Brown, and C. C. Brown. "Localization of Avian Leukosis Virus Subgroup J in Naturally Infected Chickens by RNA In Situ Hybridization." Veterinary Pathology 38, no. 6 (2001): 649–56. http://dx.doi.org/10.1354/vp.38-6-649.

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The novel subgroup J of avian leukosis virus (ALV-J) has emerged as a significant cause of myeloid neoplasia and weight suppression in broiler chickens. We investigated viral tropism using RNA in situ hybridization (ISH) in naturally infected chickens. Formalin-fixed tissues were collected from 12-day-old embryos (seven infected, two control) and from 0-week-old (four infected, one control), 3-week-old (five infected, one control), 6-week-old (five infected, one control), and 9-week-old (10 infected, two control) chickens naturally infected with ALV-J in ovo. A 636-base antisense riboprobe com

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9

Jia, Nan, Bin Li, Yuliang Zhao, et al. "Exploratory Study of Sex Identification for Chicken Embryos Based on Blood Vessel Images and Deep Learning." Agriculture 13, no. 8 (2023): 1480. http://dx.doi.org/10.3390/agriculture13081480.

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The identification of a chicken’s sex is a massive task in the poultry industry. To solve the problems of traditional artificial observation in determining sex, such as time-consuming and laborious, a sex identification method of chicken embryos based on blood vessel images and deep learning was preliminarily investigated. In this study, we designed an image acquisition platform to capture clear blood vessel images with a black background. 19,748 images of 3024 Jingfen No. 6 breeding eggs were collected from days 3 to 5 of incubation in Beijing Huadu Yukou Poultry Industry. Sixteen thousand se

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10

Moriya, K., J. T. Pearson, W. W. Burggren, A. Ar, and H. Tazawa. "Continuous measurements of instantaneous heart rate and its fluctuations before and after hatching in chickens." Journal of Experimental Biology 203, no. 5 (2000): 895–903. http://dx.doi.org/10.1242/jeb.203.5.895.

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There has been considerable interest in heart rate (fh) fluctuations in relation to cardiovascular control systems and foetal conditions during pregnancy in mammals. Prominent fluctuations in fh also occur in avian embryos, which are an important experimental model for studying developmental physiology. The present study determined the instantaneous fh of seven chick embryos continuously from the last stage of prenatal development (day 18), throughout the pipping (perinatal) period (days 19–21) until hatching and, subsequently, of newly hatched chicks (up to day 2). The distinctive patterns of

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11

Sawai, S., K. Kato, Y. Wakamatsu, and H. Kondoh. "Organization and expression of the chicken N-myc gene." Molecular and Cellular Biology 10, no. 5 (1990): 2017–26. http://dx.doi.org/10.1128/mcb.10.5.2017-2026.1990.

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We cloned the chicken N-myc gene and analyzed its structure and expression. We found that it consisted of three exons with coding regions in exons 2 and 3. Comparison to mammalian N-myc genomic sequence indicated that nucleotide sequences of the 5'-flanking region, noncoding exon 1, and introns were not conserved, but coding and 3' noncoding sequences showed significant homology to mammalian N-myc. Alignment of deduced amino acid sequences of chicken and mammalian N-myc proteins revealed nine conserved domains interrupted by different lengths of nonhomologous sequences. Two of the domains were

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12

Sawai, S., K. Kato, Y. Wakamatsu, and H. Kondoh. "Organization and expression of the chicken N-myc gene." Molecular and Cellular Biology 10, no. 5 (1990): 2017–26. http://dx.doi.org/10.1128/mcb.10.5.2017.

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We cloned the chicken N-myc gene and analyzed its structure and expression. We found that it consisted of three exons with coding regions in exons 2 and 3. Comparison to mammalian N-myc genomic sequence indicated that nucleotide sequences of the 5'-flanking region, noncoding exon 1, and introns were not conserved, but coding and 3' noncoding sequences showed significant homology to mammalian N-myc. Alignment of deduced amino acid sequences of chicken and mammalian N-myc proteins revealed nine conserved domains interrupted by different lengths of nonhomologous sequences. Two of the domains were

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13

Abala, Aline, Anna Teresa Acuña, Lojsam Daan, Jonessa Pearl Dico, Aleana Dannyze Fontillas, and Kevin Christian Dela Cruz. "The Antiangiogenic Activity of the Pereskia grandifolia (Seven Star Needle) Crude Leaf Extract Using the Chick Embryo Chorioallantoic Membrane (CAM) Assay." Recoletos Multidisciplinary Research Journal 1, Special Issue (2025): 21–30. https://doi.org/10.32871/rmrj25si.t2414.

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Background: The study focused on the anticancer potential of Pereskia grandifolia, specifically its antiangiogenic properties. Methods: Soxhlet extraction and rotary evaporation yielded a crude leaf extract containing flavonoids, phenols, tannins, steroids, and alkaloids. An ex-ovo Chorioallantoic Membrane (CAM) Assay was performed on twenty-one 4-day-old chicken embryos, divided into three groups: the experimental group (40.5 mg/50 mcL crude extract), positive control (7.5 mcg/50 mcL dexamethasone), and negative control (50 mcL distilled water). Results: The experimental group exhibited the h

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14

Uslu, Muhittin, Rahmi Canbar, Mustafa Sedat Arslan, and Harun Kızılay. "Determination of the embryotoxic effect of Metronidazole using an in ovo model." Revista Científica de la Facultad de Ciencias Veterinarias XXXIV, no. 1 (2024): 1–4. http://dx.doi.org/10.52973/rcfcv-e34310.

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Metronidazole is an imidazole group bactericidal antibiotic used against anaerobic bacteria and some protozoa. There is no detailed information about the embryotoxic effect of Metronidazole. This study aims to determine the embryotoxic activity of Metronidazole using an in ovo method. A total of 210 fertile chicken eggs were placed in an incubator, divided into seven equal groups of 30. The first group was considered as the control group. On the seventh day of the study, Metronidazole was administered to the other six groups within 50 µL saline solution at doses of 250, 125, 62.5, 31.25, and 1

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15

Mebatsion, Teshome, Leonie T. C. de Vaan, Niels de Haas, Angela Römer-Oberdörfer, and Marian Braber. "Identification of a Mutation in Editing of Defective Newcastle Disease Virus Recombinants That Modulates P-Gene mRNA Editing and Restores Virus Replication and Pathogenicity in Chicken Embryos." Journal of Virology 77, no. 17 (2003): 9259–65. http://dx.doi.org/10.1128/jvi.77.17.9259-9265.2003.

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ABSTRACT Editing of P-gene mRNA of Newcastle disease virus (NDV) enables the formation of two additional proteins (V and W) by inserting one or two nontemplated G residues at a conserved editing site (5′-AAAAAGGG). The V protein of NDV plays an important role in virus replication and is also a virulence factor presumably due to its ability to counteract the antiviral effects of interferon. A recombinant virus possessing a nucleotide substitution within the A-stretch (5′-AAgAAGGG) produced 20-fold-less V protein and, in consequence, was impaired in replication capacity and completely attenuated

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16

Cooper, Rory L., and Michel C. Milinkovitch. "In vivo sonic hedgehog pathway antagonism temporarily results in ancestral proto-feather-like structures in the chicken." PLOS Biology 23, no. 3 (2025): e3003061. https://doi.org/10.1371/journal.pbio.3003061.

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The morphological intricacies of avian feathers make them an ideal model for investigating embryonic patterning and morphogenesis. In particular, the sonic hedgehog (Shh) pathway is an important mediator of feather outgrowth and branching. However, functional in vivo evidence regarding its role during feather development remains limited. Here, we demonstrate that an intravenous injection of sonidegib, a potent Shh pathway inhibitor, at embryonic day 9 (E9) temporarily produces striped domains (instead of spots) of Shh expression in the skin, arrests morphogenesis, and results in unbranched and

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17

Hodovanyi, O. V., N. L. Chukhrai, O. I. Mrochko, and O. I. Martovlos. "Preclinical evaluation of a gel composition based on a flavonoid complex for the treatment of periodontal diseases in orthodontic patients." Zaporozhye Medical Journal 25, no. 4 (2023): 352–59. http://dx.doi.org/10.14739/2310-1210.2023.4.274894.

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The aim of the work was a preclinical assessment of acute toxicity, skin resorptive, irritant effects, cumulative and catalase activity, as well as sensitizing properties of the local gel composition “Benzidaflaziverdine” (GCB) used for the treatment of periodontal diseases in orthodontic patients. Materials and methods. 119 animals were involved in the experiment, assigned to seven main and two control groups. GCB was administered intragastrically in doses of 300–600 mg/kg and intradermally of 200 μg into the outer surface of the ear. The native solution of GCB was applied to the skin and muc

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18

Lañada, Eugene, Harvie Portugaliza, Analyn Blanza, Mercy Cheryll Espejo, and Ma Delia Pagente. "Conventional biotyping revealed Mycoplasma and antimicrobial resistant Escherichia coli and Salmonella isolates during disease outbreak in broiler farms and hatcheries in Leyte, Philippines." Annals of Tropical Research, March 23, 2022, 74–85. http://dx.doi.org/10.32945/atr4416.2022.

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A respiratory disease outbreak investigation was carried out from October to December 2015 in three broiler farms and two hatcheries in Leyte. Three- to seven-day-old chicks from farms were initially brought to CVM diagnostic laboratory for necropsy and microbial analyses. Subsequent visits to hatcheries and farms were made for random swabbing and sampling of physical facilities, equipment, vehicle, unhatched eggs, 18-day old eggs, eggshells, and day-old chicks. Chick necropsy, embryo examination, and bacteriological method for Mycoplasma, Escherichia coli, Salmonella, and Aspergillus were per

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19

Lei, Qiuxia, Xin Hu, Haixia Han, et al. "Integrative analysis of circRNA, miRNA, and mRNA profiles to reveal ceRNA regulation in chicken muscle development from the embryonic to post-hatching periods." BMC Genomics 23, no. 1 (2022). http://dx.doi.org/10.1186/s12864-022-08525-5.

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Abstract Background The growth and development of skeletal muscle are regulated by protein-coding genes and non-coding RNA. Circular RNA (circRNA) is a type of non-coding RNA involved in a variety of biological processes, especially in post-transcriptional regulation. To better understand the regulatory mechanism of circRNAs during the development of muscle in chicken, we performed RNA-seq with linear RNA depletion for chicken breast muscle in 12 (E 12) and17 (E 17) day embryos, and 1 (D 1), 14 (D 14), 56 (D 56), and 98 (D 98) days post-hatch. Results We identified 5755 differentially expresse

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20

"Using in-ovo injection of olive pulp extract and vitamin C to improve hatchability, post hatch growth performance, carcass traits and some biochemical blood analysis in broiler chickens." International Journal of Veterinary Science 12, no. 3, 2023 (2022): 353–59. http://dx.doi.org/10.47278/journal.ijvs/2022.200.

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A total of 630 fresh hatching eggs containing live embryo at 14 day of incubation were distributed randomly into seven treatment groups (three replicates/treatment, 30eggs/each) and the fertile eggs were injected in the yolk sac as following groups: eggs were un –injected (T1), eggs were injected with hole only as negative control (T2), eggs were injected with distilled water as positive control (T3), eggs were injected with OPE at 500 and 1000ppm/egg, T4 and T5), while eggs were injected with Vit C at 4 and 8mg/egg (T6 and T7) respectively. The newly hatched chicks resulting from previous tre

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