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1

Runyen-Janecky, L. J., S. A. Reeves, E. G. Gonzales, and S. M. Payne. "Contribution of the Shigella flexneri Sit, Iuc, and Feo Iron Acquisition Systems to Iron Acquisition In Vitro and in Cultured Cells." Infection and Immunity 71, no. 4 (2003): 1919–28. http://dx.doi.org/10.1128/iai.71.4.1919-1928.2003.

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ABSTRACT Shigella flexneri possesses multiple iron acquisition systems, including proteins involved in the synthesis and uptake of siderophores and the Feo system for ferrous iron utilization. We identified an additional S. flexneri putative iron transport gene, sitA, in a screen for S. flexneri genes that are induced in the eukaryotic intracellular environment. sitA was present in all Shigella species and in most enteroinvasive Escherichia coli strains but not in any other E. coli isolates tested. The sit locus consists of four genes encoding a potential ABC transport system. The deduced amin
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2

Boulette, Megan L., and Shelley M. Payne. "Anaerobic Regulation of Shigella flexneri Virulence: ArcA Regulates fur and Iron Acquisition Genes." Journal of Bacteriology 189, no. 19 (2007): 6957–67. http://dx.doi.org/10.1128/jb.00621-07.

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ABSTRACT Invasion and plaque formation in epithelial monolayers are routinely used to assess the virulence of Shigella flexneri, a causative agent of dysentery. A modified plaque assay was developed to identify factors contributing to the virulence of S. flexneri under the anaerobic conditions present in the colon. This assay demonstrated the importance of the ferrous iron transport system Feo, as well as the global transcription factors Fur, ArcA, and Fnr, for Shigella plaque formation in anoxic environments. Transcriptional analyses of S. flexneri iron transport genes indicated that anaerobi
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3

Fisher, Carolyn R., Nicola M. L. L. Davies, Elizabeth E. Wyckoff, Zhengyu Feng, Edwin V. Oaks, and Shelley M. Payne. "Genetics and Virulence Association of the Shigella flexneri Sit Iron Transport System." Infection and Immunity 77, no. 5 (2009): 1992–99. http://dx.doi.org/10.1128/iai.00064-09.

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ABSTRACT The sit-encoded iron transport system is present within pathogenicity islands in all Shigella spp. and some pathogenic Escherichia coli strains. The islands contain numerous insertion elements and sequences with homology to bacteriophage genes. The Shigella flexneri sit genes can be lost as a result of deletion within the island. The formation of deletions was dependent upon RecA and occurred at relatively high frequency. This suggests that the sit region is inherently unstable, yet sit genes are maintained in all of the clinical isolates tested. Characterization of the sitABCD genes
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4

Luck, Shelley N., Sally A. Turner, Kumar Rajakumar, Harry Sakellaris, and Ben Adler. "Ferric Dicitrate Transport System (Fec) of Shigella flexneri 2a YSH6000 Is Encoded on a Novel Pathogenicity Island Carrying Multiple Antibiotic Resistance Genes." Infection and Immunity 69, no. 10 (2001): 6012–21. http://dx.doi.org/10.1128/iai.69.10.6012-6021.2001.

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ABSTRACT Iron uptake systems which are critical for bacterial survival and which may play important roles in bacterial virulence are often carried on mobile elements, such as plasmids and pathogenicity islands (PAIs). In the present study, we identified and characterized a ferric dicitrate uptake system (Fec) in Shigella flexneriserotype 2a that is encoded by a novel PAI termed theShigella resistance locus (SRL) PAI. Thefec genes are transcribed in S. flexneri, and complementation of a fec deletion inEscherichia coli demonstrated that they are functional. However, insertional inactivation of f
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5

Runyen-Janecky, Laura, Aaron Daugherty, Benjamin Lloyd, Christopher Wellington, Haig Eskandarian, and Matthew Sagransky. "Role and Regulation of Iron-Sulfur Cluster Biosynthesis Genes in Shigella flexneri Virulence." Infection and Immunity 76, no. 3 (2008): 1083–92. http://dx.doi.org/10.1128/iai.01211-07.

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ABSTRACT Shigella flexneri, a causative agent of bacterial dysentery, possesses two predicted iron-sulfur cluster biosynthesis systems called Suf and Isc. S. flexneri strains containing deletion mutations in the entire suf operon (UR011) or the iscSUA genes (UR022) were constructed. Both mutants were defective in surviving exposure to oxidative stress. The suf mutant showed growth that was comparable to that of the parental strain in both iron-replete and iron-limiting media; however, the isc mutant showed reduced growth, relative to the parental strain, in both media. Although the suf mutant
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6

Runyen-Janecky, Laura, Ellyn Dazenski, Stephanie Hawkins, and Lisa Warner. "Role and Regulation of the Shigella flexneri Sit and MntH Systems." Infection and Immunity 74, no. 8 (2006): 4666–72. http://dx.doi.org/10.1128/iai.00562-06.

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ABSTRACT Shigella flexneri possesses at least two putative high-affinity manganese acquisition systems, SitABCD and MntH. Mutations in the genes encoding the components of both of these systems were constructed in S. flexneri. The sitA mntH mutant showed reduced growth, relative to the wild type, in Luria broth (L broth) containing the divalent metal chelator ethylene diamino-o-dihydroxyphenyl acetic acid, and the addition of either iron or manganese restored growth to the level of the wild-type strain. Although the sitA mntH mutant was not defective in surviving exposure to superoxide generat
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7

Runyen-Janecky, L. J., and S. M. Payne. "Identification of Chromosomal Shigella flexneri Genes Induced by the Eukaryotic Intracellular Environment." Infection and Immunity 70, no. 8 (2002): 4379–88. http://dx.doi.org/10.1128/iai.70.8.4379-4388.2002.

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ABSTRACT Upon entry into the eukaryotic cytosol, the facultative intracellular bacterium Shigella flexneri is exposed to an environment that may necessitate the expression of particular genes for it to survive and grow intracellularly. To identify genes that are induced in response to the intracellular environment, we screened a library containing fragments of the S. flexneri chromosome fused to a promoterless green fluorescent protein gene (gfp). Bacteria containing promoter fusions that had a higher level of gfp expression when S. flexneri was intracellular (in Henle cells) than when S. flex
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8

Lozano Aguirre, Luis Fernando, Juan Carlos Salazar, José Ignacio Vásquez, and Víctor Antonio García-Angulo. "Interdependency of regulatory effects of iron and riboflavin in the foodborne pathogen Shigella flexneri determined by integral transcriptomics." PeerJ 8 (September 15, 2020): e9553. http://dx.doi.org/10.7717/peerj.9553.

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Shigella flexneri is the causative agent of dysentery. For pathogens, iron is a critical micronutrient as its bioavailability is usually low in bacterial niches. This metal is involved in critical physiological processes mainly as a component of important metabolic molecules involved in redox reactions. Usually bacteria respond to fluctuations in iron availability to regulate iron acquisition and other iron-related functions. Recently the close metabolic feedback between iron and riboflavin, another pivotal biological redox agent, began to draw attention in bacteria. This is a widespread biolo
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9

Lucchini, Sacha, Hong Liu, Qi Jin, Jay C. D. Hinton, and Jun Yu. "Transcriptional Adaptation of Shigella flexneri during Infection of Macrophages and Epithelial Cells: Insights into the Strategies of a Cytosolic Bacterial Pathogen." Infection and Immunity 73, no. 1 (2005): 88–102. http://dx.doi.org/10.1128/iai.73.1.88-102.2005.

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ABSTRACT Shigella flexneri, the etiologic agent of bacillary dysentery, invades epithelial cells as well as macrophages and dendritic cells and escapes into the cytosol soon after invasion. Dissection of the global gene expression profile of the bacterium in its intracellular niche is essential to fully understand the biology of Shigella infection. We have determined the complete gene expression profiles for S. flexneri infecting human epithelial HeLa cells and human macrophage-like U937 cells. Approximately one quarter of the S. flexneri genes showed significant transcriptional adaptation dur
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10

Mey, Alexandra R., Elizabeth E. Wyckoff, Lindsey A. Hoover, Carolyn R. Fisher, and Shelley M. Payne. "Vibrio cholerae VciB Promotes Iron Uptake via Ferrous Iron Transporters." Journal of Bacteriology 190, no. 17 (2008): 5953–62. http://dx.doi.org/10.1128/jb.00569-08.

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ABSTRACT Vibrio cholerae uses a variety of strategies for obtaining iron in its diverse environments. In this study we report the identification of a novel iron utilization protein in V. cholerae, VciB. The vciB gene and its linked gene, vciA, were isolated in a screen for V. cholerae genes that permitted growth of an Escherichia coli siderophore mutant in low-iron medium. The vciAB operon encodes a predicted TonB-dependent outer membrane receptor, VciA, and a putative inner membrane protein, VciB. VciB, but not VciA, was required for growth stimulation of E. coli and Shigella flexneri strains
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11

Lawlor, K. M., P. A. Daskaleros, R. E. Robinson, and S. M. Payne. "Virulence of iron transport mutants of Shigella flexneri and utilization of host iron compounds." Infection and Immunity 55, no. 3 (1987): 594–99. http://dx.doi.org/10.1128/iai.55.3.594-599.1987.

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12

Pieper, Rembert, C. R. Fisher, Moo-Jin Suh, S. T. Huang, P. Parmar, and S. M. Payne. "Analysis of the Proteome of Intracellular Shigella flexneri Reveals Pathways Important for Intracellular Growth." Infection and Immunity 81, no. 12 (2013): 4635–48. http://dx.doi.org/10.1128/iai.00975-13.

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ABSTRACTGlobal proteomic analysis was performed withShigella flexneristrain 2457T in association with three distinct growth environments:S. flexnerigrowing in broth (in vitro),S. flexnerigrowing within epithelial cell cytoplasm (intracellular), andS. flexnerithat were cultured with, but did not invade, Henle cells (extracellular). Compared toin vitroand extracellular bacteria, intracellular bacteria had increased levels of proteins required for invasion and cell-to-cell spread, including Ipa, Mxi, and Ics proteins. Changes in metabolic pathways in response to the intracellular environment also
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13

Wyckoff, Elizabeth E., Alexandra R. Mey, Andreas Leimbach, Carolyn F. Fisher, and Shelley M. Payne. "Characterization of Ferric and Ferrous Iron Transport Systems in Vibrio cholerae." Journal of Bacteriology 188, no. 18 (2006): 6515–23. http://dx.doi.org/10.1128/jb.00626-06.

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ABSTRACT Vibrio cholerae has multiple iron acquisition systems, including TonB-dependent transport of heme and of the catechol siderophore vibriobactin. Strains defective in both of these systems grow well in laboratory media and in the infant mouse intestine, indicating the presence of additional iron acquisition systems. Previously uncharacterized potential iron transport systems, including a homologue of the ferrous transporter Feo and a periplasmic binding protein-dependent ATP binding cassette (ABC) transport system, termed Fbp, were identified in the V. cholerae genome sequence. Clones e
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14

Vokes, Steven A., Stephanie A. Reeves, Alfredo G. Torres, and Shelley M. Payne. "The aerobactin iron transport system genes in Shigella flexneri are present within a pathogenicity island." Molecular Microbiology 33, no. 1 (1999): 63–73. http://dx.doi.org/10.1046/j.1365-2958.1999.01448.x.

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15

Mehlman, Ira J., Aida Romero, and Barry A. Wentz. "Improved Enrichment for Recovery of Shigella sonnei from Foods." Journal of AOAC INTERNATIONAL 68, no. 3 (1985): 552–55. http://dx.doi.org/10.1093/jaoac/68.3.552.

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Abstract Shigella species were recovered from foods by the procedure described in the Bacteriological Analytical Manual, 5th Ed. The method is effective if Shigella species are present at about 106 cells/g. A 25 g food portion was incubated in Gram-negative (GN) and selenite cystine broths for 16 h at 35°C and streaked onto MacConkey, Levine's eosin methylene blue, desoxycholate citrate, and xylose lysine desoxycholate agars. 5. sonnei cells were recovered quantitatively at 44.5°C, and along with other Shigella species, were grown with Escherichia coli in a tryptone broth under anaerobic condi
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16

Ochoa, Theresa J., Eric L. Brown, Chase E. Guion, Jane Z. Chen, Robert J. McMahon, and Thomas G. Cleary. "Effect of lactoferrin on EnteroaggregativeE. coli(EAEC)This paper is one of a selection of papers published in this Special Issue, entitled 7th International Conference on Lactoferrin: Structure, Function, and Applications, and has undergone the Journal’s usual peer review process." Biochemistry and Cell Biology 84, no. 3 (2006): 369–76. http://dx.doi.org/10.1139/o06-053.

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We previously demonstrated that lactoferrin inhibits adherence of enteropathogenic Escherichia coli to HEp-2 cells and decreases invasiveness of Shigella flexneri in HeLa cells by disruption of the type III secretory system (TTSS) of both enteropathogens. To determine whether these effects were specific to the TTSS, we assessed the activity of bovine lactoferrin on enteroaggregative E. coli (EAEC), enteropathogens whose virulence is not TTSS dependent. Bovine lactoferrin at a concentration of 1.0 and 0.1 mg/mL inhibited EAEC growth. Saturation with iron reversed the bacteriostatic effect. Lact
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17

Runyen-Janecky, Laura J., Alexandria N. Brown, Brittany Ott, Haddis G. Tujuba, and Rita V. M. Rio. "Regulation of High-Affinity Iron Acquisition Homologues in the Tsetse Fly Symbiont Sodalis glossinidius." Journal of Bacteriology 192, no. 14 (2010): 3780–87. http://dx.doi.org/10.1128/jb.00161-10.

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ABSTRACT Sodalis glossinidius is a facultative intracellular bacterium that is a secondary symbiont of the tsetse fly (Diptera: Glossinidae). Since studies with other facultative intracellular bacteria have shown that high-affinity iron acquisition genes are upregulated in vivo, we investigated the regulation of several Sodalis genes that encode putative iron acquisition systems. These genes, SG1538 (hemT) and SG1516 (sitA), are homologous to genes encoding periplasmic heme and iron/manganese transporters, respectively. hemT promoter- and sitA promoter-gfp fusions were constructed, and in both
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18

MAROLDA, C. L., M. A. VALVANO, K. M. LAWLOR, S. M. PAYNE, and J. H. CROSA. "Flanking and Internal Regions of Chromosomal Genes Mediating Aerobactin Iron Uptake Systems in Enteroinvasive Escherichia coli and Shigella flexneri." Microbiology 133, no. 8 (1987): 2269–78. http://dx.doi.org/10.1099/00221287-133-8-2269.

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19

Derbyshire, P., T. Baldwin, P. Stevenson, et al. "Expression in Escherichia coli K-12 of the 76,000-dalton iron-regulated outer membrane protein of Shigella flexneri confers sensitivity to cloacin DF13 in the absence of Shigella O antigen." Infection and Immunity 57, no. 9 (1989): 2794–98. http://dx.doi.org/10.1128/iai.57.9.2794-2798.1989.

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20

Afridi, Ome Kalsoom, Johar Ali, and Jeong Ho Chang. "Next-Generation Sequencing Based Gut Resistome Profiling of Broiler Chickens Infected with Multidrug-Resistant Escherichia coli." Animals 10, no. 12 (2020): 2350. http://dx.doi.org/10.3390/ani10122350.

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The study was designed to investigate the fecal microbiome and resistome of broiler chickens infected with multidrug-resistant (MDR) Escherichia coli (E. coli). Fecal samples (n = 410) from broiler chickens were collected from thirteen randomly selected sites of Khyber Pakhtunkhwa and screened for the presence of MDR E. coli. Upon initial screening, thirteen (13) MDR E. coli isolates were then subjected to shotgun metagenome next-generation sequencing (NGS). NGS based resistome analysis identified the multidrug efflux pump system-related genes at the highest prevalence (36%) followed by aminog
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21

Griffiths, E., P. Stevenson, T. L. Hale, and S. B. Formal. "Synthesis of aerobactin and a 76,000-dalton iron-regulated outer membrane protein by Escherichia coli K-12-Shigella flexneri hybrids and by enteroinvasive strains of Escherichia coli." Infection and Immunity 49, no. 1 (1985): 67–71. http://dx.doi.org/10.1128/iai.49.1.67-71.1985.

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22

Jalili, Mahsa, Mansour Amraei, Nourkhoda Sadeghifard, and Sobhan Ghafourian. "Evaluation of Biofilm Formation and Anti-biofilm Properties of Peganum Harmala and Crocus Sativus in Shigella Flexneri Clinical Isolates." Open Microbiology Journal 13, no. 1 (2019): 297–300. http://dx.doi.org/10.2174/1874285801913010297.

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Background: Biofilm formation causes many serious problems in the treatment of bacterial infections. In addition, chronic infections due to biofilm formation can pose a huge burden to the health care systems. Also, many bacteria are biofilm producers as an important strategy for pathogenicity. Furthermore, the traditional use of herbal medicines such as Peganum harmala and Crocus sativus in Iran is interesting. Objective: The purpose of the current study was to investigate the biofilm formation in Shigella flexneri clinical isolates and to evaluate the anti-biofilm properties of P. harmala and
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23

Ranjbar, Reza, Mohammad Pourshafie, and Mohammad Dallal. "The study of antimicrobial resistance among Shigella flexneri strains isolated in Tehran, Iran." Journal of Pediatric Infectious Diseases 06, no. 02 (2015): 125–29. http://dx.doi.org/10.3233/jpi-2011-0307.

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24

Rajabi, Z., M. S. Dallal, M. Pourshafie, R. Ranjbar, and S. Heidarzadeh. "943 The Study of Antimicrobial Resistance Among Shigella Flexneri Strains Isolated of Children in Tehran, Iran." Archives of Disease in Childhood 97, Suppl 2 (2012): A270. http://dx.doi.org/10.1136/archdischild-2012-302724.0943.

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25

Tarhriz, Vahideh, Shirin Eyvazi, Elia Shakeri, Mohammad Saeid Hejazi, and Azita Dilmaghani. "Antibacterial and Antifungal Activity of Novel Freshwater Bacterium Tabrizicola aquatica as a Prominent Natural Antibiotic Available in Qurugol Lake." Pharmaceutical Sciences 26, no. 1 (2020): 88–92. http://dx.doi.org/10.34172/ps.2019.56.

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Background : Recently, resistant pathogenic microorganisms have become increasingly wide spread. The search for new natural antibiotics is a viable solution to this problem. For this aim we investigated the antimicrobial ability of Tabrizicola aquatica, the novel bacterium isolated from Qurugol Lake located nearby Tabriz city, Iran. Methods: The antimicrobial properties of Tabrizacola aquatica was investigated using well diffusion test. Tabtizicola aquatica was incubated at 40℃ in shaking incubator at 150 rpm for 14 days. The culture was centrifuged to obtain cell free supernatant, which was s
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26

"Shigella enterotoxin 1 (ShET1): An iron-regulated enterotoxin elbaroated by Shigella Flexneri 2a active in rabbit small intestine." Gastroenterology 108, no. 4 (1995): A284. http://dx.doi.org/10.1016/0016-5085(95)23797-6.

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27

Moradi, Farhad, Nahal Hadi, Maryam Akbari, Zahra Hashemizadeh, and Reyhaneh Rouhi Jahromi. "Frequency and Antimicrobial Resistance of Shigella Species in Iran During 2000 - 2020." Jundishapur Journal of Health Sciences 13, no. 2 (2021). http://dx.doi.org/10.5812/jjhs.114902.

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Context: Numerous studies have shown the high frequency and antibiotic-resistant patterns of Shigella species in different provinces of Iran. In this study, we performed a comprehensive review from 2000 to 2020 in Iran to describe the prevalence rate and antibiotic-resistant patterns of S. sonnei, S. dysenteriae, S. flexneri, and S. boydii. Evidence Acquisition: We systematically searched the biomedical databases including Scopus, Google Scholar, PubMed, SID, and Web of Science for related articles published in English or Persian. Finally, out of 70 articles, 34 studies were included in the st
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28

Mahdian, Nahid, Ebrahim Kouhsari, Ali Gheysarzadeh, Abbas Maleki, Hassan Valadbeigi, and Nourkhoda Sadeghifard. "The Prevalence of Shiga toxin-1 in non-Shigella dysenteriae isolates collected from diarrhea samples in patients, Ahvaz, Iran." Infectious Disorders - Drug Targets 20 (December 7, 2020). http://dx.doi.org/10.2174/1871526520666201207122924.

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Background: Acute diarrhea is a major public health problem particularly in developing countries. Shigellosis is one of the substantial causative agents of microbial dysentery and still has a remarkable prevalence particularly in areas with poor hygienic infrastructures. The probable existence of the deadly Shiga toxin (Stx) protein in some Shigella strains would manifest life-threatening clinical symptoms of the infection. Methods: The aim of this study was to determine the presence of Shigella toxin 1 (Stx1) in isolated from patients with diarrhea. Totally, 227 Shigella species including 60
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Karkoub, Maryam, Ebrahim Kouhsari, Soheila Khaghani, and Nourkhoda Sadeghifard. "Clonal Lineage Analysis of Shigella flexneri Isolates Circulating in Ahvaz, Iran." Clinical Laboratory 67, no. 04/2021 (2021). http://dx.doi.org/10.7754/clin.lab.2020.200830.

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30

Teimourpour, Roghayeh, Behnam Babapour, Majid Esmaelizad, Mohsen Arzanlou, and Hadi Peeri-Doghaheh. "Molecular characterization of quinolone resistant Shigella spp. isolates from patients in Ardabil, Iran." Iranian Journal of Microbiology, January 14, 2020. http://dx.doi.org/10.18502/ijm.v11i6.2221.

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Background and Objectives: Shigella is an etiological agent of shigellosis. Antibiotic therapy has a critical role in decreas- ing serious complications of shigellosis. The present study aimed to determine the multi-drug resistance strains and to detect fluoroquinolone related mutations.
 Materials and Methods: In this descriptive, cross sectional study, a total of 113 Shigella isolates were collected from 1280 patients admitted to Bu-Ali hospital in Ardabil province during 2015-17. Antibiotic resistance pattern of isolates was evalu- ated using Kirby Bauer method and finally, the MICs of
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