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1

Nógrády, Noémi, Margit Király, Klára Borbás, Ákos Tóth, Judit Pászti, and István Tóth. "Antimicrobial resistance and genetic characteristics of integron-carrier shigellae isolated in Hungary (1998–2008)." Journal of Medical Microbiology 62, no. 10 (2013): 1545–51. http://dx.doi.org/10.1099/jmm.0.058917-0.

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Antimicrobial susceptibility, integron carriage, genetic relationship and presence of some important virulence genes of the integron-carrier strains of Shigella sonnei (n = 230) and Shigella flexneri (n = 22) isolated from stool samples of patients in Hungary between 1998 and 2008 were investigated. Sixty-seven per cent (168/252) of the strains were resistant to sulfamethoxazole/trimethoprim (SxT) followed by streptomycin (S, 47 %), ampicillin (A, 32 %) and tetracycline (Tc, 28 %). Thirty-six per cent (90/252) exhibited multidrug resistance, mostly showing SSxTTc or ASSxTc, ASSxTTc resistance
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2

Makarova, M. A., Z. N. Matveeva, L. A. Kaftyreva, et al. "Characteristics of susceptibility to antibiotics of Shigella sonnei caused three outbreaks in the North-Western region of Russia in 2018." Bacteriology 5, no. 2 (2020): 18–23. http://dx.doi.org/10.20953/2500-1027-2020-2-18-23.

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The work is devoted to the study of susceptibility to antibiotics of 42 strains of Shigella sonnei isolated from patients involved in three food-borne outbreaks that occurred in the North-Western region of Russia in 2018. All strains belonged to biovar IIg, showed resistance to tetracycline and trimethoprim/sulfamethoxazole. The strains were susceptibility to nitrofurantoin, ciprofloxacin, but were resistant to nalidixic acid, were susceptibility to aminoglycosides (gentamycin, tobramycin, and amikacin), and resistance to streptomycin. All strains were susceptibility to azithromycin (MIC 8 mg/
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3

Bondarenko, A. P., T. A. Zaitseva, O. E. Trotsenko, et al. "SONNEI DYSENTERY MORBIDITY IN KHABAROVSK AND KHABAROVSK REGION DUE TO ATYPICAL MANNITOL-NEGATIVE CAUSATIVE AGENT." Journal of microbiology epidemiology immunobiology, no. 1 (February 28, 2017): 20–28. http://dx.doi.org/10.36233/0372-9311-2017-1-20-28.

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Aim. Determine features of epidemic process (EP) of Sonnei dysentery in Khabarovsk Region in 2012 - 2014 due to atypical causative agent. Materials and methods. Detailed characteristics of 161 cultures of Shigella sonnei isolated from 81 patients from epidemic focus in children boarding school in Bikin as well as from 22 patients from sporadic and group foci of dysentery in Khabarovsk (biochemical type, colicin-genotype, spectrum of drug resistance) is given. Molecular-biologic subtyping was carried out for 11 strains by Pulsed Field Gel Electrophoresis method (PFGE). Results. Materials of obs
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June, Geraldine A., Patricia S. Sherrod, R. Miguel Amaguana, Wallace H. Andrews, and Thomas S. Hammack. "Effectiveness of the Bacteriological Analytical Manual Culture Method for the Recovery of Shigella sonnei from Selected Foods." Journal of AOAC INTERNATIONAL 76, no. 6 (1993): 1240–48. http://dx.doi.org/10.1093/jaoac/76.6.1240.

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Abstract The relative retention of the indigenous morphological, biochemical, and serological characteristics by Shigella sonnei was tested under various storage conditions (room temperature, refrigeration, freezing at −20°C and at −70°C, and lyophilization). The use of a selective (desoxycholate citrate) agar rather than a nonselective (brain heart infusion) agar gave a lower conversion rate of smooth to rough colonies, and the percentage of rough colonies derived from cultures stored for prolonged periods increased under all conditions. With respect to biochemical characteristics, there were
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Rodríguez, Edna Catering, Adriana Marcela Bautista, Lucy Angeline Montaño, María Victoria Ovalle, and Francia Patricia Correa. "Laboratory-based surveillance of Shigella spp. from human clinical cases in Colombia, 1997-2018." Biomédica 41, no. 1 (2021): 65–78. http://dx.doi.org/10.7705/biomedica.5113.

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Introduction: Shigellosis is endemic in low-and middle-income countries, causing approximately 125 million episodes of diarrhea and leading to approximately 160 .000 deaths annually one-third of which is associated with children.Objective: To describe the characteristics and antimicrobial resistance profiles of Shigella species recovered in Colombia from 1997 to 2018.Materials and methods: We received isolates from laboratories in 29 Colombian departments. We serotyped with specific antiserum and determined antimicrobial resistance and minimal inhibitory concentrations for ten antibiotics with
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6

Grenón, S., L. Leguizamón, A. Del Valle, S. Pallares, M. Salvi Grabulosa, and M. von Specht. "Prevalence and antimicrobial profile of Shigella causing acute diarrheal disease in pediatrics in Posadas, Misiones." Revista de Ciencia y Tecnología, no. 38 (October 31, 2022): 71–75. http://dx.doi.org/10.36995/j.recyt.2022.38.009.

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Our objective was to evaluate the presence and microbiological characteristics of Shigella in children with acute diarrhoeal disease (ADD), attended at the Paediatric Hospital of Posadas, Misiones between 2016 and 2019. All ADEs documented in the laboratory were included. Standardised microbiological techniques were used. We considered age groups: infants (L)≤ 2 years, preschool (PE): 3-5 years, school (E): 5- 14 years. We recorded 106/2128 (5%) Shigella spp. isolates, with a higher frequency in summer (54.5%). S. flexneri (64; 60.4% serotypes 02(54), 03(3), AA479(2) and 01(1)) and S. sonnei (
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7

Jones, Nick K., Trang Nguyen Hoang Thu, Ruklanthi de Alwis, et al. "The seroincidence of childhood Shigella sonnei infection in Ho Chi Minh City, Vietnam." PLOS Neglected Tropical Diseases 17, no. 10 (2023): e0011728. http://dx.doi.org/10.1371/journal.pntd.0011728.

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Background Shigella sonnei is a pathogen of growing global importance as a cause of diarrhoeal illness in childhood, particularly in transitional low-middle income countries (LMICs). Here, we sought to determine the incidence of childhood exposure to S. sonnei infection in a contemporary transitional LMIC population, where it represents the dominant Shigella species. Methods Participants were enrolled between the age of 12–36 months between June and December 2014. Baseline characteristics were obtained through standardized electronic questionnaires, and serum samples were collected at 6-month
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Vasileva, O. V., A. S. Volynkina, I. V. Kuznetsova, S. V. Pisarenko, and A. N. Kulichenko. "MOLECULAR-GENETIC CHARACTERISTICS OF SHIGELLA SONNEI-2013 STRAIN ISOLATED DURING THE OUTBREAK IN DYSENTERY IN THE REPUBLIC ABKHAZIA IN 2013." Journal of microbiology epidemiology immunobiology, no. 1 (February 28, 2018): 72–76. http://dx.doi.org/10.36233/0372-9311-2018-1-72-76.

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Aim. Study of molecular-genetic properties of Shigella sonnei-2013 strain isolated during the outbreak in dysentery in the republic Abkhazia in 2013. Materials and methods. Genetic typing of the tested strains using multilocus sequence typing (MLST). Analyzed of nucleotide sequence fragments 7 of conservative «housekeeping» genes adk, fumC, icd, mdh, purA, recA, gyrB. Sequenced of DNA fragments compared with reference sequences from database of Escherichia coli MLST. Phylogenetic analysis was performed using UPGMA method and computer program START 2. Whole-genome sequencing performed on a gene
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WU, F. M., M. P. DOYLE, L. R. BEUCHAT, J. G. WELLS, E. D. MINTZ, and B. SWAMINATHAN. "Fate of Shigella sonnei on Parsley and Methods of Disinfection." Journal of Food Protection 63, no. 5 (2000): 568–72. http://dx.doi.org/10.4315/0362-028x-63.5.568.

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Outbreaks of shigellosis associated with chopped parsley used as a garnish for foods occurred in four states in the United States and in two Canadian provinces in 1998. This prompted a study to determine survival and growth characteristics of Shigella sonnei inoculated onto raw parsley. Two inoculum levels (∼103 and 106 CFU/g) were applied to parsley leaves, portions of which were then chopped. Inoculated whole and chopped parsley leaves were held at 4°C or 21°C for up to 14 days. Initial populations of the organism on chopped parsley receiving high or low levels of inoculum increased by appro
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Nundwe, Mike, Joseph Yamweka Chizimu, John Mwaba, et al. "Antimicrobial Resistance Patterns and Serological Diversity of Shigella Species from Patient Isolates at University Teaching Hospital in Lusaka, Zambia." Bacteria 4, no. 2 (2025): 18. https://doi.org/10.3390/bacteria4020018.

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Background: Shigella species are the leading cause of human shigellosis. In Zambia, more than 30% of children experiencing diarrhea are infected with Shigella species. The increasing resistance of Shigella species to the recommended therapy is of great concern. Therefore, this study investigated the antibiotic resistance profiles and phenotypic and genotypic characteristics of Shigella isolates at the largest referral hospital in Zambia. Methodology: Of the forty-eight archived presumptive Shigella isolates, thirty-two were serologically confirmed and subjected to antimicrobial susceptibility
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Cristea, Daniela, Mihaela Oprea, Adriana Simona Ciontea, Felicia Antohe, and Codruta-Romanita Usein. "Prevalence of virulence markers and pHS-2-like plasmids among Shigella sonnei and Shigella flexneri isolates originating from shigellosis cases in Romania." Revista Romana de Medicina de Laborator 24, no. 1 (2016): 103–10. http://dx.doi.org/10.1515/rrlm-2016-0003.

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AbstractThe surveillance of shigellosis is carried out under the auspice of the European Centre for Disease Prevention and Control which requires a reliable laboratory-based surveillance at national level. To date, little information is published about the members of Shigella spp. responsible for Romanian cases of shigellosis which hinders the understanding of the current epidemiology of shigellosis. Consequently, this retrospective study aimed to assess the diversity of virulence profiles displayed by the strains circulating in our region, by using key chromosome- and plasmid-associated marke
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12

Bose, Puja, Goutam Chowdhury, Gourab Halder, et al. "Prevalence and changing antimicrobial resistance profiles of Shigella spp. isolated from diarrheal patients in Kolkata during 2011–2019." PLOS Neglected Tropical Diseases 18, no. 2 (2024): e0011964. http://dx.doi.org/10.1371/journal.pntd.0011964.

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Background The primary aim of this study was to investigate the occurrence, characteristics, and antimicrobial resistance patterns of various Shigella serotypes isolated from patients with acute diarrhea of the Infectious Diseases Hospital in Kolkata from 2011–2019. Principal findings During the study period, Shigella isolates were tested for their serotypes, antibiotic resistance pattern and virulence gene profiles. A total of 5.8% of Shigella spp. were isolated, among which S. flexneri (76.1%) was the highest, followed by S. sonnei (18.7%), S. boydii (3.4%), and S. dysenteriae (1.8%). Antimi
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NYGREN, B. L., K. A. SCHILLING, E. M. BLANTON, B. J. SILK, D. J. COLE, and E. D. MINTZ. "Foodborne outbreaks of shigellosis in the USA, 1998–2008." Epidemiology and Infection 141, no. 2 (2012): 233–41. http://dx.doi.org/10.1017/s0950268812000222.

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SUMMARYWe examined reported outbreaks of foodborne shigellosis in the USA from 1998 to 2008 and summarized demographic and epidemiological characteristics of 120 confirmed outbreaks resulting in 6208 illnesses. Most reported foodborne shigellosis outbreaks (n = 70, 58%) and outbreak-associated illnesses (n = 3383, 54%) were restaurant-associated. The largest outbreaks were associated with commercially prepared foods distributed in multiple states and foods prepared in institutional settings. Foods commonly consumed raw were implicated in 29 (24%) outbreaks and infected food handlers in 28 (23%
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14

Hong, Seong Jin, Chang Hoon Lee, Jun Ho Wang, Wonkeun Song та Suk Hoon Jung. "Clinical Characteristics of Extended-spectrum β-lactamase Producing Shigella sonnei Infection Outbreaked in Chungju Area". Annals of Laboratory Medicine 26, № 3 (2006): 168–73. http://dx.doi.org/10.3343/kjlm.2006.26.3.168.

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15

AGBABIAKA, Toyin Olayemi, and Blessing Olubunmi OLOFINTOYE. "Microbial Diversity in Water and Biofilm Samples from Well Sources in Ilorin Metropolis, Nigeria." Notulae Scientia Biologicae 11, no. 1 (2019): 56–62. http://dx.doi.org/10.15835/nsb11110337.

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The aim of the study was to investigate the microbial populations of water and biofilm samples from seven hand dug wells in Ilorin, Nigeria, which are for private and public use. Physicochemical parameters such as pH, turbidity, total dissolved solids, temperature, conductivity, dissolved oxygen and biological oxygen demand were determined. Biofilm growths were induced from water samples obtained from selected wells. Twenty bacteria isolated were identified using morphological and biochemical characteristics as well as molecular methods and includes Shigella sonnei, Shigella dysenteriae, Micro
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NA-UBOL, M., S. SAMOSORNSUK, L. VON SEIDLEIN, et al. "Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand." Epidemiology and Infection 134, no. 5 (2006): 997–1003. http://dx.doi.org/10.1017/s0950268806005899.

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In this study, we used plasmid profile analysis, XbaI macrorestriction with pulsed-field gel electrophoresis (PFGE), and PCR of the ipaH gene, to study the molecular characteristics of 183 Shigella spp. isolated during May 2000 to April 2003 from rectal swabs of patients with watery and/or bloody diarrhoea in a new industrialized area of Thailand. Among the 183 isolates, 167 were S. sonnei and 16 were S. flexneri. For plasmid profile analysis, the 183 isolates revealed 16 different plasmid patterns, designated patterns A to P. The sizes of the plasmid bands were: 6, 5·5, 5, 4·5, 4, 3·25, 2·75,
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Pan, Jing-Cao, Rong Ye, Dong-Mei Meng, Wei Zhang, Hao-Qiu Wang, and Ke-Zhou Liu. "Molecular characteristics of class 1 and class 2 integrons and their relationships to antibiotic resistance in clinical isolates of Shigella sonnei and Shigella flexneri." Journal of Antimicrobial Chemotherapy 58, no. 2 (2006): 288–96. http://dx.doi.org/10.1093/jac/dkl228.

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Oliveira Lima, Karina, Cristian Mauricio Barreto Pinilla, Ailén Alemán, et al. "Characterization, Bioactivity and Application of Chitosan-Based Nanoparticles in a Food Emulsion Model." Polymers 13, no. 19 (2021): 3331. http://dx.doi.org/10.3390/polym13193331.

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In this study, chitosan nanoparticles (CNPs) were prepared by the ionic gelation technique with tripolyphosphate (TPP), and the effect of CNP composition and physicochemical characteristics were evaluated. After the synthesis optimization, CNPs were used as carriers for a fish protein hydrolysate (FPH) with bioactive properties (CNPH). The physicochemical characteristics, antioxidant capacity and antimicrobial, antihypertensive and emulsifier properties of unloaded and loaded CNPs in a food system model were studied. CNPH showed a uniform particle distribution, size ~200 nm, high stability (ze
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Ibrahim, Aaliya F., Kathryn Glass, Deborah A. Williamson, et al. "The changing epidemiology of shigellosis in Australia, 2001–2019." PLOS Neglected Tropical Diseases 17, no. 3 (2023): e0010450. http://dx.doi.org/10.1371/journal.pntd.0010450.

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Shigellosis is an increasing cause of gastroenteritis in Australia, with prolonged outbreaks reported in remote Aboriginal and Torres Strait Islander (hereafter “First Nations”) communities and among men who have sex with men (MSM) in major cities. To determine associations between Shigella species and demographic and geographic factors, we used multivariate negative binomial regression to analyse national case notifications of shigellosis from 2001 to 2019. Between 2001 and 2019, Australian states and territories reported 18,363 shigellosis cases to the National Notifiable Diseases Surveillan
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KOUTSOTOLI, A. D., M. E. PAPASSAVA, V. E. MAIPA, and Y. P. ALAMANOS. "Comparing Shigella waterborne outbreaks in four different areas in Greece: common features and differences." Epidemiology and Infection 134, no. 1 (2005): 157–62. http://dx.doi.org/10.1017/s0950268805004747.

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SUMMARYIn Greece the public health surveillance for most infectious diseases, including shigellosis, is not well developed. However, four waterborne outbreaks of shigellosis have been recorded in Greece in the last three decades, in different areas of the country, under different environmental and socioeconomic conditions. This study presents the epidemiological characteristics of these four outbreaks in order to examine common points and differences and to investigate their possible association with environmental and socioeconomic conditions. All four outbreaks were caused by Shigella sonnei
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Munoz, Carlos, James G. Olson, James R. Murphy, et al. "Characteristics of Shigella sonnei Infection of Volunteers: Signs, Symptoms, Immune Responses, Changes in Selected Cytokines and Acute-Phase Substances." American Journal of Tropical Medicine and Hygiene 53, no. 1 (1995): 47–54. http://dx.doi.org/10.4269/ajtmh.1995.53.47.

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Rana, Md Masud, and Md Anwar Ul Islam. "Isolation, Characterization and Antimicrobial Activities of the Metabolites from Different Sources of Bangladesh." Bangladesh Pharmaceutical Journal 20, no. 2 (2018): 172–79. http://dx.doi.org/10.3329/bpj.v20i2.37882.

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Antibiotic resistance and emergence of new infections are becoming serious health concern in the recent days. To overcome these problems, the present study was designed to isolate and characterize new microbial species from different locations of Rajshahi division, Bangladesh that can produce bioactive metabolites effective against resistant microorganisms. A total of 56 microbial isolates were obtained by cross streaking and agar disc diffusion method and tested against Bacillus cereus, Staphylococcus aureus ATCC-259233, Listeria monocytogenes, Agrobacterium spp., Escherichia coli FPFC-1407,
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Shetty, Akshata, and Manish Bhat. "Preliminary Analysis of Soy Curd by Natural Fermentation Using Green Chilies." International Journal of Current Microbiology and Applied Sciences 13, no. 7 (2024): 43–53. http://dx.doi.org/10.20546/ijcmas.2024.1307.006.

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Plant-based soy yogurt, as an alternative to traditional dairy yogurt, has gained widespread acceptance owing to its nutritional benefits and versatility. This study investigated soy curd prepared with various starting materials, including chili, cow yogurt starter, and buffalo yogurt starter, and evaluated their bacterial counts in CFU/ml. Significant variations in bacterial counts were observed among different preparations. The Soy curd milk was fermented naturally with the use of green chilies. For this pH was found to be 5.5±0.2, with 1.325g/L TA. The CFU count was found to be 1.1×106. The
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Joshi, Sneha, Shreya Baniya, Ashis Shrestha, Sumana Bajracharya, Piyush Rajbhandari, and Sushmita Joshi. "Demographic profile of patient with acute watery diarrhea during monsoon 2022: Patan Hospital, Nepal." Journal of Patan Academy of Health Sciences 10, no. 2 (2023): 65–71. http://dx.doi.org/10.3126/jpahs.v10i2.59283.

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Introduction: Diarrhea is the passage of three or more loose or liquid stools per day or more. Acute watery diarrhea is a major public health problem worldwide. In Nepal, diarrhea is among the top 10 inpatient morbidity. This study’s objective is to monitor demographic characteristics and laboratory findings of stool specimens of diarrhea. Method: This is a cross-sectional descriptive study done at Patan Hospital. Ethical approval was taken from the Ethical committee. Data for Monsoon 2022 (June to September 2022) were collected from the Patan Hospital record system. Cases from within the Lali
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Gao, Qi, Qiuhong Liao, Qingming Li, Qichang Yang, Fang Wang, and Jianming Li. "Effects of LED Red and Blue Light Component on Growth and Photosynthetic Characteristics of Coriander in Plant Factory." Horticulturae 8, no. 12 (2022): 1165. http://dx.doi.org/10.3390/horticulturae8121165.

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Coriander is a whole-plant edible micro vegetable frequently used in the food industry. Its fresh eating features give it a flavor that is both tasty and refreshing, as well as potentially dangerous due to the bacteria (e.g., Shigella sonnei) it may contain. Artificial light-based plant factories are becoming increasingly popular due to the development of light-emitting diodes (i.e., LEDs). These plant factories employ artificial light to recreate the ideal lighting conditions for photosynthesis, ensuring plant yield and safety. Red (R) light and blue (B) light are essential for crop developme
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Schaffner, Donald W., Dane Jensen, Charles P. Gerba, David Shumaker, and James W. Arbogast. "Influence of Soap Characteristics and Food Service Facility Type on the Degree of Bacterial Contamination of Open, Refillable Bulk Soaps." Journal of Food Protection 81, no. 2 (2018): 218–25. http://dx.doi.org/10.4315/0362-028x.jfp-17-251.

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ABSTRACT Concern has been raised regarding the public health risks from refillable bulk-soap dispensers because they provide an environment for potentially pathogenic bacteria to grow. This study surveyed the microbial quality of open refillable bulk soap in four different food establishment types in three states. Two hundred ninety-six samples of bulk soap were collected from food service establishments in Arizona, New Jersey, and Ohio. Samples were tested for total heterotrophic viable bacteria, Pseudomonas, coliforms and Escherichia coli, and Salmonella. Bacteria were screened for antibioti
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Koilybayeva, Moldir, Zhanserik Shynykul, Gulbaram Ustenova, et al. "Molecular Characterization of Some Bacillus Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency." Molecules 28, no. 7 (2023): 3210. http://dx.doi.org/10.3390/molecules28073210.

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Numerous natural habitats, such as soil, air, fermented foods, and human stomachs, are home to different Bacillus strains. Some Bacillus strains have a distinctive predominance and are widely recognized among other microbial communities, as a result of their varied habitation and physiologically active metabolites. The present study collected vegetable products (potato, carrot, and tomato) from local markets in Almaty, Kazakhstan. The bacterial isolates were identified using biochemical and phylogenetic analyses after culturing. Our phylogenetic analysis revealed three Gram-positive bacterial
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Nakayama, Shu-ichi, Akira Kushiro, Takashi Asahara, et al. "Activation of hilA expression at low pH requires the signal sensor CpxA, but not the cognate response regulator CpxR, in Salmonella enterica serovar Typhimurium." Microbiology 149, no. 10 (2003): 2809–17. http://dx.doi.org/10.1099/mic.0.26229-0.

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A two-component regulatory system, cpxR–cpxA, plays an important role in the pH-dependent regulation of virF, a global activator for virulence determinants including invasion genes, in Shigella sonnei. The authors examined whether the cpxR–cpxA homologues have some function in the expression of Salmonella enterica serovar Typhimurium invasion genes via the regulation of hilA, an activator for these genes. In a Salmonella cpxA mutant, the hilA expression level was reduced to less than 10 % of that in the parent strain at pH 6·0. This mutant strain also showed undetectable synthesis of an invasi
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Musa, Muhammad, Farzana Gul Jan, Muhammad Hamayun, et al. "An Endophytic Fungal Isolate Paecilomyces lilacinus Produces Bioactive Secondary Metabolites and Promotes Growth of Solanum lycopersicum under Heavy Metal Stress." Agronomy 13, no. 3 (2023): 883. http://dx.doi.org/10.3390/agronomy13030883.

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Heavy metal (HM) contamination in the soil and accumulation in plants poses a serious threat to crops and human health. HMs such as cadmium, mercury, lead and arsenic are widely acknowledged for their adverse effects on plants such as chlorosis, necrosis, low food quality and crop yields. Endophytic fungi secrete secondary metabolites and enhance the plant’s ability to tolerate stressful conditions. However, the role of most fungal endophytes in their host plant growth or production of metabolites under HM stress conditions needs further understanding. In the present study, we studied the HM s
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Andrade-Ochoa, Sergio, Karla Fabiola Chacón-Vargas, Luvia Enid Sánchez-Torres, Blanca Estela Rivera-Chavira, Benjamín Nogueda-Torres, and Guadalupe Virginia Nevárez-Moorillón. "Differential Antimicrobial Effect of Essential Oils and Their Main Components: Insights Based on the Cell Membrane and External Structure." Membranes 11, no. 6 (2021): 405. http://dx.doi.org/10.3390/membranes11060405.

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The biological activity of essential oils and their major components is well documented. Essential oils such as oregano and cinnamon are known for their effect against bacteria, fungi, and even viruses. The mechanism of action is proposed to be related to membrane and external cell structures, including cell walls. This study aimed to evaluate the biological activity of seven essential oils and eight of their major components against Gram-negative and Gram-positive bacteria, filamentous fungi, and protozoans. The antimicrobial activity was evaluated by determination of the Minimal Inhibitory C
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Altikriti, Shahad. "Determination of the Activity of the Green Synthesized Silver Nanoparticles Against Multidrug Resistant Bacteria Isolated from Children with Diarrhea under Five Years." INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 2, no. 1 (2019): 75–76. http://dx.doi.org/10.32441/ijms.2.1.9.

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Background: Bacterial resistance to wide range of antibiotics increased dramatically in the last decade. The most serious of such resistant is the multidrug resistance that leads to failure of treatment and increase in morbidity and mortality.Aim: To evaluate the synergistic inhibition of combination of nano particles with antibiotics against multidrug resistant bacteria.Materials and methods: One hundred twenty stool specimens were collected from children under five years old with diarrhea. These samples were collected during the period from May 2015 to October 2015 from different hospitals i
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Andreeva, Irina S., Aleksandr S. Safatov, Larisa I. Puchkova, et al. "Occurrence and characteristics of Bacillus cereus group bacterial atmospheric aerosols in Novosibirsk region." Vestnik Tomskogo gosudarstvennogo universiteta. Biologiya, no. 56 (2021): 60–85. http://dx.doi.org/10.17223/19988591/56/3.

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The microbial diversity of atmospheric bioaerosols involves microorganisms that can cause allergic and infectious diseases or toxic effects. They include bacteria of the Bacillus cereus group (B. cereus, B. thuringiensis, B. anthracis, B. mycoides, B. pseudomycoides, etc.), which can result in diarrhea, pneumonia, meningitis, septicemia, and other infectious diseases. Accordingly, monitoring the presence of Bacillus cereus group bacteria in aerosols is critical. However, practically no data exist on Bacillus cereus and other cereus-group bacteria in southwestern Siberia’s poorly investigated a
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Trivedi, Mahendra. "Evaluation of Phenotyping and Genotyping Characteristic of Shigella sonnei after Biofield Treatment." OMICS International, June 19, 2017. https://doi.org/10.5281/zenodo.813436.

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Shigella sonnei (S. sonnei) is a non-motile, rod shape, clinically significant, Gram-negative bacterium. It is commonly associated with dysentery (shigellosis). Recently, resistance to third and fourth generation cephalosporins and fluoroquinolones has been reported in S. sonnei. In the present study, we assessed the effect of biofield treatment on phenotyping and genotyping characteristic of S. sonnei (ATCC 9290). The lyophilized samples of S. sonnei were divided in three groups (G): G-I (control, revived), G-II (treatment, revived), and G-III (treatment, lyophilized). All these groups (contr
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Bardsley, Megan, Claire Jenkins, Holly D. Mitchell, et al. "Persistent Transmission of Shigellosis in England Is Associated with a Recently Emerged Multidrug-Resistant Strain of Shigella sonnei." Journal of Clinical Microbiology 58, no. 4 (2020). http://dx.doi.org/10.1128/jcm.01692-19.

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ABSTRACT Whole-genome sequencing has enhanced surveillance and facilitated detailed monitoring of the transmission of Shigella species in England. We undertook an epidemiological and phylogenetic analysis of isolates from all cases of shigellosis referred to Public Health England between 2015 and 2018 to explore recent strain characteristics and the transmission dynamics of Shigella species. Of the 4,950 confirmed cases of shigellosis identified during this period, the highest proportion of isolates was Shigella sonnei (54.4%), followed by S. flexneri (39.2%), S. boydii (4.1%), and S. dysenter
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Stefanovic, Aleksandra, Mosaab E. Alam, Nancy Matic, et al. "Increased severity of multidrug-resistant Shigella sonnei infections in people experiencing homelessness." Clinical Infectious Diseases, November 21, 2024. http://dx.doi.org/10.1093/cid/ciae575.

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Abstract Background Shigella sonnei has caused sexually transmitted enteric infections in men who have sex with men (MSM) in Vancouver. We recently observed a high rate of multidrug-resistant (MDR) S. sonnei bacteremia among persons experiencing homelessness (PEH). We aim to describe the wider epidemiology, clinical outcomes, and genomics of S. sonnei infections over time. Methods A retrospective review of 163 patients with S. sonnei infections was undertaken from 2015 –2022. We collected demographic, clinical, and microbiological data over two time periods: historical (2015-2020) and recent (
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Cohen, Dani, Orit Treygerman, Shifra Ken-Dror, et al. "Twenty-five years of sentinel laboratory-based surveillance of shigellosis in a high-income country endemic for the disease, Israel, 1998 to 2022." Eurosurveillance 29, no. 31 (2024). http://dx.doi.org/10.2807/1560-7917.es.2024.29.31.2400022.

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Background Shigella is a leading cause of moderate-to-severe diarrhoea worldwide and diarrhoeal deaths in children in low- and-middle-income countries. Aim We investigated trends and characteristics of shigellosis and antimicrobial resistance of Shigella sonnei in Israel. Methods We analysed data generated by the Sentinel Laboratory-Based Surveillance Network for Enteric Pathogens that systematically collects data on detection of Shigella at sentinel laboratories, along with the characterisation of the isolates at the Shigella National Reference Laboratory. Trends in the shigellosis incidence
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Charles, Hannah, Katy Sinka, Ian Simms, Kate S. Baker, Gauri Godbole, and Claire Jenkins. "Trends in shigellosis notifications in England, January 2016 to March 2023." Epidemiology and Infection 152 (2024). http://dx.doi.org/10.1017/s0950268824001006.

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Abstract We reviewed all diagnoses of Shigella species notified to the UK Health Security Agency from January 2016 to March 2023. An overall increase in notifications of shigellosis was seen between 2016 (n = 415/quarter) and 2023 (n = 1 029/quarter). However, notifications dramatically declined between March 2020 and September 2021 during the COVID-19 pandemic (n = 208/quarter) highlighting the impact of travel and social distancing restrictions on transmission. S. sonnei diagnoses were more affected by lockdown restrictions than S. flexneri, most likely due to a combination of species-specif
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"Isolation, Identification and Evaluation of Antibiotic Sensitivity and Resistance of Shigella Dysenteriae Isolated from Food, Water and Soil." Journal of Microbiological Sciences JMS, August 19, 2022, 5–10. http://dx.doi.org/10.38211/jms.2022.01.01.02.

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Background: Shigella is a genus of gram negative, facultative anerobic rod shaped bacteria divided into four species that are Shigella dysenteriae (serogroup A), Shigella flexneri (serogroup B), Shigella boydii (serogroup C) and Shigella sonnei (serogroup D). Shigella species are the etiological agent of shigellosis and most serious illnesses are caused by Shigella dysenteriae. Aim: The aim of this study was to determine antibiotic sensitivity and resistance of Shigella dysenteriae isolates to ensure that severe diarrhea infection remain treatable. Method: A total of 75 samples from different
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Huang, Mengying, Xiaoxuan Zhang, Chaochen Luo, Haibin Xu, Yufeng Qiu, and Jinsong Yang. "Genome and antibiotic resistance characteristics of Shigella clinical isolates in Fujian Province, Southeast China, 2005–2019." Microbial Genomics 10, no. 11 (2024). http://dx.doi.org/10.1099/mgen.0.001325.

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Shigellosis is a serious public health issue in many developing countries. The emergence of multidrug-resistant (MDR) Shigella isolates has deepened the treatment difficulty and health burden of shigellosis. China is the largest developing country in the world, but so far, the genome of MDR Shigella isolates has not been well characterized. In this study, 60 clinical isolates of Shigella spp. in Fujian Province, southeast China, from 2005 to 2019 were characterized for drug resistance phenotype, whole-genome sequencing and bioinformatics analysis. The results showed that the MDR rate of Shigel
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Nguyen, Dong Tu, Masatomo Morita, Tuan Cuong Ngo, et al. "Characterization of Shigella flexneri in northern Vietnam in 2012–2016." Access Microbiology 5, no. 6 (2023). http://dx.doi.org/10.1099/acmi.0.000493.v4.

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Introduction. Shigellosis remains a considerable public health concern in developing countries. Shigella flexneri and Shigella sonnei are prevalent worldwide and S. sonnei has been replacing S. flexneri . Gap Statement. S. flexneri still causes outbreaks of shigellosis in northern Vietnam but limited information is available on its genetic characteristics. Aim. This study aimed to characterize the genetic characteristics of S. flexneri strains from northern Vietnam. Methodology. This study used 17 isolates from eight incidents, collected in northern Vietnam between 2012 and 2016. The samples w
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Rahajamanana, Lalaina V., Paulin Andrianjakasolo, Dera S. Andriatahiana, Zakasoa Ravaoarisaina, Liliane J. Raboba, and Andry Rasamindrakotroka. "Laboratory confirmed shigellosis in Malagasy children." International Journal of Research in Medical Sciences, May 14, 2022. http://dx.doi.org/10.18203/2320-6012.ijrms20221329.

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Background: Diarrheal diseases are a major public health problem in developing countries with high mortality and morbidity rates, especially among children. Shigella species is the leading cause of paediatric bacterial diarrhea and shigellosis data are very scarce in Madagascar.Methods: A 3 year retrospective study from January 2018 to December 2020, at the University Hospital Mother and Child Tsaralalàna laboratory was performed to assess the bacteriological and epidemiological characteristics of laboratory confirmed shigellosis cases.Results: During the study period, 298 stool samples were e
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Liu, Wei, Jia-Wei Tang, Jing-Yi Mou, et al. "Rapid discrimination of Shigella spp. and Escherichia coli via label-free surface enhanced Raman spectroscopy coupled with machine learning algorithms." Frontiers in Microbiology 14 (March 8, 2023). http://dx.doi.org/10.3389/fmicb.2023.1101357.

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Shigella and enterotoxigenic Escherichia coli (ETEC) are major bacterial pathogens of diarrheal disease that is the second leading cause of childhood mortality globally. Currently, it is well known that Shigella spp., and E. coli are very closely related with many common characteristics. Evolutionarily speaking, Shigella spp., are positioned within the phylogenetic tree of E. coli. Therefore, discrimination of Shigella spp., from E. coli is very difficult. Many methods have been developed with the aim of differentiating the two species, which include but not limited to biochemical tests, nucle
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Hmar, El Bethel Lalthavel, Sujata Paul, and Hemanta Kumar Sharma. "The role of Shigella spp. in propagating bacillary dysentery in humans and the prominence of nanotechnology in disease prevention." Future Journal of Pharmaceutical Sciences 10, no. 1 (2024). http://dx.doi.org/10.1186/s43094-024-00676-4.

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Abstract Background Shigellosis, also known as bacillary dysentery, is an acute infection of the intestine. The symptoms can vary from mild watery diarrhoea to severe inflammatory bacillary dysentery, which is characterized by fever, intense abdominal cramps, and the presence of blood and mucus in the stools. While the disease typically resolves on its own, it can become life-threatening in immunocompromised individuals or in the absence of adequate medical care. Main body of the abstract Shigella is the primary cause of bacillary dysentery worldwide. It is comprised of four distinct species—S
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Liao, Ying-Shu, Bo-Han Chen, Yu-Ping Hong, et al. "The rise in domestic shigellosis and the genomic characteristics of Shigella clones linked to men who have sex with men in Taiwan, 2015‒2022." Microbiology Spectrum, February 13, 2025. https://doi.org/10.1128/spectrum.02290-24.

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ABSTRACT Since 2015, Taiwan has experienced a notable rise in domestic shigellosis cases, particularly among young adult males. In this study, we aimed to investigate this epidemiological trend through demographic analysis and genomic characterization of bacterial isolates. We analyzed demographic data on shigellosis cases from 2003–2014 to 2015–2022. For cases from 2015–2022, we conducted genomic analyses of Shigella isolates using pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing (WGS). Antimicrobial resistance and plasmid profiles were examined to identify genetic determin
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Benmessaoud, R., I. Jroundi, M. Nezha, et al. "Aetiology, epidemiology and clinical characteristics of acute moderate-to-severe diarrhoea in children under 5 years of age hospitalized in a referral paediatric hospital in Rabat, Morocco." Journal of Medical Microbiology (J Med Microbiol.), January 9, 2015. https://doi.org/10.1099/jmm.0.079830-0.

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The objective of the study was to describe the aetiology, epidemiology and clinical characteristics of the principal causes of acute infectious diarrhoea requiring hospitalization among children under 5 years of age in Rabat, Morocco. A prospective study was conducted from March 2011 to March 2012, designed to describe the main pathogens causing diarrhoea in hospitalized children >2 months and less than 5 years of age. Among the 122 children included in the study, enteroaggregative Escherichia coli (EAEC) and rotavirus were the main aetiological causes of diarrhoea detected. Twelve (9.8 %)
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Liao, Feng, Jing Qian, Ruian Yang, et al. "Metagenomics of gut microbiome for migratory seagulls in Kunming city revealed the potential public risk to human health." BMC Genomics 24, no. 1 (2023). http://dx.doi.org/10.1186/s12864-023-09379-1.

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Abstract Background Seagull as a migratory wild bird has become most popular species in southwest China since 1980s. Previously, we analyzed the gut microbiota and intestinal pathogenic bacteria configuration for this species by using 16S rRNA sequencing and culture methods. To continue in-depth research on the gut microbiome of migratory seagulls, the metagenomics, DNA virome and RNA virome were both investigated for their gut microbial communities of abundance and diversity in this study. Results The metagenomics results showed 99.72% of total species was bacteria, followed by viruses, fungi
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Botelho-Nevers, E., and P. Gautret. "Outbreaks associated to large open air festivals, including music festivals, 1980 to 2012." Eurosurveillance 18, no. 11 (2013). http://dx.doi.org/10.2807/ese.18.11.20426-en.

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In the minds of many, large scale open air festivals have become associated with spring and summer, attracting many people, and in the case of music festivals, thousands of music fans. These festivals share the usual health risks associated with large mass gatherings, including transmission of communicable diseases and risk of outbreaks. Large scale open air festivals have however specific characteristics, including outdoor settings, on-site housing and food supply and the generally young age of the participants. Outbreaks at large scale open air festivals have been caused by Cryptosporium par
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Samuel, Soi K., Ngeiywa M. Moses, and Too J. Emily. "Prevalence of Enterobacteriaceae Isolated from Childhood Diarrhoea in Mukuru Slums, Nairobi- Kenya." Journal of Advances in Microbiology, July 25, 2019, 1–9. http://dx.doi.org/10.9734/jamb/2019/v17i330142.

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Diarrhoea in young children continues to be a major public health concern in developing countries, including Kenya. Poor sanitation among other factors can predispose a child to diarrhoea. Therefore, the present study sought to determine the prevalence of enterobacteriaceae isolated from childhood diarrhoea in Mukuru Slums, Nairobi. It employed a cross-sectional design targeting children below 5 years of age. Stool specimens were obtained aseptically and cultured on MacConkey agar and Salmonella-Shigella agar. Biochemical tests were used to identify the isolated bacteria to genus and species u
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Mohammad, Al Sayasneh, Olena Ruban, Inna Kovalevska, and Natalia Khokhlenkova. "Selection of antimicrobial substances in the rectal cream composition containing thick extract of carrot and rutin." Annals of Mechnikov Institute, March 19, 2024, 13–17. https://doi.org/10.5281/zenodo.10838408.

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<strong>Introduction</strong>. Synthetic preservatives can significantly extend the shelf life of a perishable product, although they have many side effects that are harmful to human health. In view of this, there is a current trend towards the use of preservatives made from natural plant products. According to the literature, natural substances are widely used as effective preservatives. Regulatory authorities that control the quality, safety and efficacy of medicines pay great attention to natural substances that can be used in the pharmaceutical industry, which will reduce the risk of side
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