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1

Khanam, Tahmina, and Gulshan Ara Latifa. "Hematological Parameter of Heteropneustes fossilis (Shing fish)." Journal of Science Foundation 11, no. 1 (2013): 3–10. http://dx.doi.org/10.3329/jsf.v11i1.19398.

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Background: The physiological conditions of fish are essential for the successful fish culture. Objective: The purpose of the present study was to see the haematological parameter of air breathing cat fish (Heteropneustes fossilis) of Bangladesh. Methodology: This animal study was conducted in the “Bargen lab” in the Department of Zoology at University of Dhaka as well as in “The Peoples Pathological lab” which was a private diagnostic laboratory at Dhaka city from July’ 2008 to April’ 2009 for a period of 9(nine) months. The fish was Heteropneustes fossilis. Haemocytometer including two graduated pipettes was used for counting leucocytes. Result: The result indicated eight types of blood cells in peripheral condition of Heteropneustes fossilis. The average cellular counts of Heteropneustes fossilis were erythrocytes 8.45´106 m3, leukocytes 15.44´103m-3, Thrombocytes 34.72%, large lymphocytes 1.02%, small lymphocytes 26.7%, monocyte 3.9%, neutrophil 16.9%, eosinophils 6.97%, basophiles 8.6%, haemoglobin 11.7g 100ml1, erythrocyte sedimentation rate (ESR) 6.49g 100ml-1. The most of the hematological parameter showed intra specific variation except eosinophils and ESR of Heteropneustes fossilis (Shing fish). Conclusion: Hematological studies shows that the physiology of fish change with the change in the environment, time, season, maturity, nutritional state, activity level physical and chemical change in water.DOI: http://dx.doi.org/10.3329/jsf.v11i1.19398
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2

Islam, Nahidul, Moumita Choudhury, Md Sadiqur Rahman, Md Jamadul Islam, M. Anisur Rahman, and M. Aminur Rahman. "Identification and antimicrobial sensitivity profiling of bacteria isolated from cultured catfish shing (Heteropneustes fossilis)." Journal of Biological Studies 5, no. 3 (2022): 604–17. http://dx.doi.org/10.62400/jbs.v5i3.7081.

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The cultivated species of catfish (Heteropneustes fossilis), also known as "Shing" has a delectable flavor, a high market value, a high nutritional and therapeutic value, and a high iron and calcium content. In Bangladesh, the shing (H. fossilis) fish is one of the most sought-after, popular, and extremely valuable indigenous species. The purpose of this research was to isolate bacterial pathogens from infected shing (H. fossilis) fish and determine their antibiotic sensitivity. In total, 76 medically infected shing fish were collected by the cultivators from their own ponds in the Jashore district, in southern Bangladesh. Disease-causing bacteria were found in 62 (81.5%) of the infected fish, while normal flora were found in 14 (18.5%) of the 76 samples. There were 62 pathogenic bacterial isolates found, and Aeromona spp., Pseudomonas spp., Streptococcus spp., Flavobacterium spp. and Vibrio spp. were the main causes of disease in the sick fish. Among the species of bacteria that were isolated, the prevalence of the largest pathogens, Aeromonas spp. was 34 (51.4%) and the prevalence of the second largest, Pseudomonas spp. was 13 (20.3%). The other isolates were comprised of the following species: Streptococcus spp. 9 (9.4%), Flavobacterium spp. 4 (5.4%) and Vibrio spp. 2 (4.1%). The farming of shing (H. fossilis) fish has been increased rapidly all around the country. Therefore, bacterial infections may reduce the production of fish in ponds. In this research, bacterial strains showed a high degree of sensitivity to ciprofloxacin (77%) and levofloxacin (54%). Significantly, oxytetracycline (52%) enrofloxacin (45%) and erythromycin (45%) were the intermediate/moderate sensitive antibiotics. All the strains were found to be resistant to amoxicillin 57/62 (92%) and neomycin 42/62 (68%). The findings of this research will be beneficial to fish farmers in the treatment of bacterial infections in fish, as these diseases are caused by microorganisms. The PCR method might be studied further as a means of identifying all bacterial cultures, which would require additional research.
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3

Uddin, Md Asek, Md Ariful Islam, Rashedul Islam, Rabeya Yesmin, KM Abdul Halim, and KM Hasanuzzaman. "Study on health status of farmed shing (Heteropneustes fossilis) in Mymensingh region." Research in Agriculture Livestock and Fisheries 6, no. 3 (2020): 445–55. http://dx.doi.org/10.3329/ralf.v6i3.44811.

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Health status investigation of shing (Heteropneustes fossilis) was carried out through clinical and histopathological observations. During four months from January 2018 to April 2018. The data were collected from eight fish farms. Clinical signs of fish were physical deformity, reddish whitish lesions, abrasion and reduced mucus in the months of January and February from the investigated fish farms. Clinically fish were almost normal in the months of March and April. Histopathlogically observed pathological changes in gill were lamellar missing, splitted gill lamellae, hemorrhage, hypertrophy, vaccums and presence of parasites in January and February. In case of liver the examined fish had hemorrhage, vaccums, necrosis and fat bodies also in the months of January and February. Organs of the examined fishes were almost normal in the months of March and April. The purpose of the study was to determine the health status of farmed shing. The main objectives of the study were to observe clinical and histopathological changes in liver and gill of farmed shing (H. fossilis) at various fish farms of Mymensingh region and to determine the health status of the shing in the investigated months. From the present study, it was found that external organs like gill were more affected compared to internal organ like liver. From the present investigation, it was also observed that the fish were more affected in the months of January and February i.e. in winter season.
 Res. Agric., Livest. Fish.6(3): 445-455, December 2019
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4

ASHRAFULLAH, MD, MITHUN KARMAKAR, SHAYLA SULTANA MELY, MD TARIQUL ISLAM, and MD SHARIFUL ISLAM. "Integrated farming of stinging catfish (Heteropneustes fossilis) and rabbit (Oryctolagus cuniculus) in farmer’s pond." Bangladesh Journal of Fisheries 35, no. 1 (2023): 25–33. http://dx.doi.org/10.52168/bjf.2023.35.03.

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A study was carried out to evaluate the growth and production performances of stinging catfish (Heteropneustes fossilis) and rabbit (Oryctolagus cuniculus) under two treatments such as T1 (shing-rabbit integrated system) and T2 (shing monoculture) in farmers’ pond. Twelve ponds from two villages such as Sutiakhali and Salakandi under Mymensingh sadar upazila were selected for the study. The average pond size was 5.5±0.23decimal with a stocking density of 600 shing dec-1. The ponds were only fertilized with rabbit dung except at the time of pond preparationat a rate of 1 kg dec-1. Various environmental parameters such as temperature (oC), transparency (cm), PH, dissolve oxygen (mg L-1), PO4-P (mg L-1), NO3-N (mg L-1), NO2-N (mg L-1), NH3-N (mg L-1), chlorophyll and alkalinity (mg L-1) were monitored once in a week. The parameters were found to vary little from pond to pond and most of the parameters were within the suitable range for freshwater aquaculture in Bangladesh. The highest survival rate of fish was 95.36 ± 0.15 % in treatment T1 and the lowest was 90.39 ± 0.10 % in treatment T2. The survival rates of fish were not significantly different among the treatments (P > 0.05). The production of fish in T1 and T2 treatment was 25.36 and 20.43 kg dec-1, respectively. Daily growth of shing in two treatments were 1.95 ± 0.01 and 1.88 ± 0.01 % respectively. The study revealed that integrated shing-rabbit would be highly profitable using improve extensive culture system in the rural household ponds through effectively reducing the use of pond fertilizer and fish feeding cost.
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5

Ahamed, Saokat, Khandaker Rashidul Hasan, Yahia Mahmud, and Maliha Hossain Mou. "Culture Technique of Shing with Other High-Value Fish Species in the Semi-Arid Zone of Bangladesh." Transylvanian Review of Systematical and Ecological Research 26, no. 3 (2024): 81–90. https://doi.org/10.2478/trser-2024-0019.

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Abstract This study was conducted in farmer’s ponds within the northern region of Bangladesh to observe the growth and yield of Shing, Heteropneustes fossilis under a polyculture system. The highest total production of fish registered among the treatments in pattern-1 was 6,331 kg ha−1 with a benefit-cost ratio of 1.52. The best combinations were chosen for multi-location testing (MLT) to verify the previous results. After five months of multi-location testing of the selected combinations with Shing as the main species, the significantly (P < 0.05) highest production of Shing (5,828 kg ha−1), total production of fishes (7,352 kg ha−1) and benefit-cost ratio (1.73) were found.
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6

Rahman, Md Mamunur, Debashis Kumar Mondal, Md Rurul Amin, and Masum Billah. "Effects of stocking density on growth and production of shing (Heteropneustes fossilis) in ponds." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 2 (2016): 380–85. http://dx.doi.org/10.3329/aajbb.v1i2.61606.

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To assess the effects of stocking density on growth and production of shing (Heteropneustes fossilis)in ponds, an experiment was carried out for a period from 06 August to 22 December, 2015. Three stocking densities used were 80, 160 and 240 fish /decimal and designated as T1, T2 and T3, respectively. Each treatment has two replications. Shing having a mean body weight of 1.18g was used in T1, T2 and T3, respectively. A commercial feed fed at the rate of 50% of body weight up to first 15 days and then gradually it was readjusted to 35%, 25%, 15%, 5% and 2% respectively. The water quality parameters were monitored at 15 days interval and the ranges were: temperature 23.0 ºC to 30.25 ºC, pH 6.64 to 8.16, dissolved oxygen 4.91 to 6.99 mg/l and transparency 36.15 to 41.5 cm. Results showed that, the fish stocked at the rate of 80 fish/decimal in T1 get best individual weight gain (42.63g) followed by T2(38.52g) and T3 (34.82g), respectively. The SGR ranged between (1.77to1.81) %/day. There was significant variation (P<0.01) among the survival rate which ranged from 66.45% to 88.75%. The production was 3.02 kg, 4.96 kg and 5.55 kg/dec/134 days in T1, T2 and T3, respectively. But the highest production of 5.55kg/dec/134 days was obtained in T3 with stocking density of 240 fish/dec might be due to higher stocking density. Although the highest production was obtained in T3 but individual growth performance of shing (Heteropneustes fossilis) was highest in T1. The highest net profit was found (BDT 2591) in treatment T2 compared to treatments T1 (BDT 1800) and T3 (BDT 1479). The cost benefit ratios were 1.59, 1.48 and 1.10 % in T1, T2 and T3 respectively. Asian Australas. J. Biosci. Biotechnol. 2016, 1 (2), 380-385
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7

DAS, RAKHI, MD SHARIFUL ISLAM, and KHAN KAMAL UDDIN AHMED. "Polyculture potentials of Galda (Macrobrachium rosenbergii) with Heteropneustes fossilis and Clarias batrachus in South-western coastal ghers of Bangladesh." Bangladesh Journal of Fisheries 32, no. 1 (2020): 29–38. http://dx.doi.org/10.52168/bjf.2020.32.04.

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Prawn (Macrobrachium rosenbergii) culture is socially more acceptable, technically and economically viable and sustainable. The freshwater catfishes Heteropneustes fossilis (shing) and Clarias batrachus (magur) adapt well to hypoxic water bodies and high stocking densities as well. So, shing and magur may be a good candidate to culture with prawn in ponds/ghers to save the farmers from losing their investment in case of shrimp mortality and getting additional income from fish. With this view, this study was conducted to evaluate the feasibility of polyculture with three treatments each with three replications. Stocking density of shing was variable i.e., 200nos/decimal in T1, 300nos/decimal in T2 and 400 nos/decimal in T3. On the other hand, stocking density of magur and prawn juveniles was 50 and 30 nos/decimal respectably for all treatments. The experimental ghers were treated with salt (NaCl) (150g/decimal), potasium permanganate (5g/decimal) along with lime (125 kg/ha). After stocking fish were fed using floating nursery feed containing 32%, protein and 7% lipid at the rate of 10-2% for shing and magur nursing, floating oil coated grower feed (27% protein and 6% lipid) at the rate of 6-2% for shing and magur grow out and prawn grower (30% protein and 7% lipid) at the rate of 6-2.5% of estimated fish and prawn biomass. At least 10% of stocked catfish and galda were sampled fortnightly using cast net. Physico-chemical parameters of the experimental ghers water were monitored at weekly. After six months of culture the highest growth performance (47.0 g) and survival (19.94%) for shing obtained from T3. Whereas the average growth of shing was recorded 37.02 and 41.14g; growth of magur 99.21g and 99.51g and growth of galda 69.74 and 70.19 for Treatment T1 and T2 respectively. Except ammonia and iron content the other observed parameters of ghers water found congenial for fish health. The value of Benefit-Cost Ratio (BCR) was 1.4 that seems to be profitable and feasible of this polyculture practice.
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8

Kohinoor, AHM, MM Khan, S. Yeasmine, P. Mandol, and MS Islam. "Effects of Stocking Density on Growth and Production Performance of Indigenous Stinging Catfish, Heteropneustes fossilis (Bloch)." International Journal of Agricultural Research, Innovation and Technology 2, no. 2 (2013): 9–14. http://dx.doi.org/10.3329/ijarit.v2i2.14009.

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An on-farm experiment was undertaken in nine earthen ponds to evaluate the growth and production potentials of stinging catfish shing, Heteropneustes fossilis for the period of six months from March to August 2010. Three stocking densities such as 1,25,000 (T1), 1,87,500 (T2) and 2,50,000 ha-1 (T3) were tested with three replications each. Fish were fed with commercial pelleted feed containing 35% crude protein. After six months rearing, the mean harvesting weights of shing were 69.42±6.20, 58.74±3.85 and 49.50±4.52g in T1, T2 and T3, respectively. Significant (P<0.05) highest mean harvesting weight was found in T1. The best survival was found in T1 (87%) among the treatments. The calculated mean production of shing (H. fossilis) in three treatments such as T1, T2 and T3 were 7549±52, 9031±71 and 8786±60 kg ha-1, respectively, which were significantly different (p<0.05) from each other. DOI: http://dx.doi.org/10.3329/ijarit.v2i2.14009 Int. J. Agril. Res. Innov. & Tech. 2 (2): 9-14 December, 2012
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9

Ahmed, Gias Uddin, Md Mamunur Rahman, Mohammad Nurul Alam, and Md Ashraful Alam. "Trial of sex reverse percentage on female Shing (heteropneustes fossilis) by using 17?-Estradiol sex hormone." Research in Agriculture Livestock and Fisheries 2, no. 2 (2015): 313–17. http://dx.doi.org/10.3329/ralf.v2i2.25016.

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A study was conducted to evaluate the production of monosex (female) shing (Heteropneustes fossilis ) by using a sex hormone (17?-Estradiol). The experiment was composed of three treatments T1 (70 mg 17?-Estradiol /kg feed), T2 (80 mg 17? Estradiol /kg feed) and T3 (90mg 17?- Estradiol /kg feed) in duplicate were fed shing fishes in cemented cistern. The hormone was administered orally at variable doses with Tiger brand nursery fish feed (Eon Group) for 30 days. The fry were fed with the hormone mixed feed five times in a day. Every seven days intervals 50% water of the tanks were changed. A high mortality rate (47.6% to 67.4%) was observed in different treatments. The occurrence of female fish at the end of the experiment in treatment TI, T2 and T3 were 75%, 86%, and 96% female respectively. T3 (90 mg 17?-Estradiol /kg, feed) showed the best performance with 96% sex reversal to female progeny.Res. Agric., Livest. Fish.2(2): 313-317, August 2015
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10

Islam, MT, K. Mostafa, and MM Rashid. "Histopathological Studies of Experimentally Infected Shing, Heteropneustes fossiliswith Aeromonas hydrophila Bacteria." Progressive Agriculture 19, no. 1 (2013): 89–96. http://dx.doi.org/10.3329/pa.v19i1.17359.

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Heteropneustes fossilis (shing) of 35g body weight were experimentally infected with Aeromonas hydrophila by two different methods: viz. intraperitonial and intramuscular injection. A standard dose of infection (6.4 × 107 CFU/ml) was selected based on predetermined LD50. Each method gave rise to the mortality of shing up to 85%. Clinical signs of fish included injury, hemorrhage and large ulcerative lesions on the body at the injected area. In kidney, liver and intestine pathologically, massive atrophy and focal necrosis were found. Hemorrhage, Vacuolation and atrophy of hepatic sinusoids represented by necrosis of the sinusoidal lining cells, degeneration of hepatic tissue and distribution of bacterial cell all over the tissue were found in liver. Atrophy, hemorrhage, villi missing and missing of epithelium were found in intestine of the experimentally infected shing. Bacterial cells were distributed in the whole hematopoietic tissue including the renal tubules. Tissue abscess characterized by focal necrosis, hemorrhage and Vacuolation were also found in the kidney of the infected shing. But the above symptoms were not found in the organs of the apparently healthy shing species.DOI: http://dx.doi.org/10.3329/pa.v19i1.17359 Progress. Agric. 19(1): 89 - 96, 2008
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11

Ahammed, Tanvir, Shuvho Chakra Borty, Md Shirajum Monir, Nazneen Begum, Md Alimul Islam, and SM Lutful Kabir. "Isolation, identification and molecular detection of Aeromonas hydrophila from diseased stinging catfish shing (Heteropneustes fossilis)." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 1 (2016): 125–33. http://dx.doi.org/10.3329/aajbb.v1i1.61545.

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Fish play a crucial role in the Bangladeshi diet, providing more than 60% of animal source food, representing a crucial source of micro-nutrients, and possessing an extremely strong cultural attachment. In this study isolation and identification of Aeromionashydrophila was done by studying cultural properties, gram staining and biochemical properties of isolates of Shing fish (Heteropneustesfossilis) of different upazillas of Mymensingh district. Antibiogram profile of the isolated bacteria was studied by using wide range of commercially available antibiotics.Quantitative study of bacteria isolated from diseased Shing fish showed variation of number in different organ. Total bacterial load was found to be 1.90 × 105, 1.19 × 105, 3.21 × 105, 2.18 × 106 and 3.14 × 105cfu/g in lesions; 2.52 × 107, 2.34 × 108, 5.41 × 108, 2.54 × 109 and 5.21 × 109cfu/g in liver; 2.54 × 108, 2.41 × 108, 1.90 × 107, 3.65 × 107 and 3.45 × 108cfu/g in spleen; 3.51 × 107, 5.28 × 107, 3.14 × 106, 1.85 × 107 and 4.52× 107cfu/g in kidney in diseased Shing of Mymensingh sadar, Muktagacha, Tarakanda, Gouripur and Fulpurupazillas, respectively under Mymensingh districts. Aeromonashydrophilawas initially identified by their specific morphological, physiological and biochemical characteristics. Then molecular detection of A. hydrophila was done by PCR. PCR products of desired 760 bp were obtained for A. hydrophila. The results of the antibiotic sensitivity test is exhibited that most of the bacterial samples were sensitive against ciprofloxacin (92%) and levofloxacin (84%), intermediate resistant against gentamicin (40%) and resistant against novobiocin (84%), ampicillin (100%) and penicillin (92%). Asian Australas. J. Biosci. Biotechnol. 2016, 1 (1), 125-133
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12

Mohammad, Zakerin Abedin, Hasan Rubait, Sadiqur Rahman Md., et al. "Detachment, distinguishing proof of bacterial pathogens from infected Shing (Heteropneustes fossilis) cultured in freshwater ponds in Bangladesh." International Journal of Biomolecules and Biomedicine (IJBB) 12, no. 3 (2021): 10–16. https://doi.org/10.5281/zenodo.8330491.

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Among the local fishes, Shing (<em>Heteropneustes fossilis</em>) is one of the most demandable, popular and highly valuable fish in Bangladesh. A total of 84 clinically infected shing fishes were directly collected by a cultivator from their own ponds between April 2019 and December 2019. In total, eighty four fish-based ponds, 58(69.1%) were in Mymensingh region and the rest 26(30.9%) were in Netrakona districts in Bangladesh. Out of 84 infected fish samples, 74(88.1%) were infected with pathogenic bacteria and 10(11.9%) were with normal flora. A total of 74 pathogenic bacterial strains were isolated and among the isolates&nbsp;<em>Aeromonas&nbsp;</em>spp<em>, Pseudomonas&nbsp;</em>spp<em>, Staphylococcus&nbsp;</em>spp<em>, Citobacter&nbsp;</em>spp<em>,&nbsp;</em>and<em>&nbsp;Vibrio&nbsp;</em>spp<em>,</em>&nbsp;appeared to be the main pathogen in the diseased fishes. Among the isolated species of bacteria distribution of the largest pathogens&nbsp;<em>Aeromonas&nbsp;</em>species was 38 (51.4%), and second the largest<em>&nbsp;Pseudomonas&nbsp;</em>spp was 15(20.3%). The rest of isolates were distributed<em>&nbsp;as Staphylococcus&nbsp;</em>spp 7(9.4%)<em>, Citobacter&nbsp;</em>spp 4(5.4%),<em>&nbsp;Vibrio&nbsp;</em>spp 3(4.1%) and only 7(9.4%) others namely Bacillus spp<em>,&nbsp;</em><em>Edwardsiella spp,&nbsp;</em><em>Enterococcus&nbsp;</em>spp<em>, Flavobacterium&nbsp;</em>spp<em>, Klebsiella&nbsp;</em>spp in infected&nbsp;<em>H. fossilis</em>. The cultivation of shing (<em>H. fossilis</em>) fishes is dramatically increased all over the country. However, bacterial diseases may influence to decrease the production in ponds water. In this work, bacterial pathogens were sensitive against Ciprofloxacin (77%), Cotrimoxazole (97.3%), and Enorfloxacin (97.8%). All the strains showed resistant to 74/74(100%) Amoxicillin, and 63/74(85.1) erythromycin. The intermediate sensitive against Colistin was 35.1% and Doxycycline was 22.9% respectively. Published by the<strong>&nbsp;International Journal of Biomolecules and Biomedicine (IJBB)&nbsp;</strong>
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Rashid, MM, MA Hasan, K. Mostafa, and MA Islam. "Isolation of Aeromonas hydrophila from EUS Affected Shing Heteropneustes fossilis of a Fish Farm in Mymensingh." Progressive Agriculture 19, no. 1 (2013): 117–24. http://dx.doi.org/10.3329/pa.v19i1.17362.

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Aeromonas hydrophila bacteria was isolated from the suspected EUS-affected shing fish, Heteropneustes fossilis (Bloch). The disease investigations were primarily based on clinical signs and subsequently confirmed by the isolation of bacterial pathogen Aeromonas hydrophila from lesion of liver and kidney. The A. hydrophila isolates were identified by a series of morphological, physiological and biochemical tests. The total bacterial load in liver, intestine and kidney were 1.67 × 104 to 6.46 × 108 CFU/g, 1.71 × 103 to 1.18 × 109 CFU/g and 1.47 × 104 to 3.70 × 108 CFU/g respectively.DOI: http://dx.doi.org/10.3329/pa.v19i1.17362 Progress. Agric. 19(1): 117 - 124, 2008
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Monir, Md Shirajum, Mohammad Ashaf-Ud-Doulah, Md Khalilur Rahman, Jubaida Nasreen Akhter, Md Rayhan Hossain, and Sayeeda Sultana. "Histoarchitecture changes in the ovary of Stinging catfish, Shing (Heteropneustes fossilis) under cypermethrin toxicity." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 1 (2016): 47–53. http://dx.doi.org/10.3329/aajbb.v1i1.61530.

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Histoarchitecture effect of cypermethrin (pyrethroids) was investigated in the ovary of Stinging catfish, Shing (Heteropneustes fossilis) over a 96 h exposure period as an endpoint of toxicity. The fish were exposed to five acute concentrations (0.00ml/L, 0.025ml/L, 0.050ml/L, 0.075ml/L and 0.10ml/L) and the 96 h LC50 was 0.075 ml/L. The abnormal behavioural patterns noticed in the treated fish were erratic swimming, respiratory distress, discolorations of the skin, loss of reflex, hyperactivities, increasing opercula ventilation and excessive accumulation of mucus on the skin. There was an atretic follicle, cytoplasmic liquefaction, degeneration ovarian wall, invasion of granulosa layer and small inter-follicular spaces after treatment of 0.025 mg/L cypermethrin. Vacuolization at periphery, complete or partial rupture of maturing oocytes, vacuolization of oocytes, degenerating oocytes with disintegrated nuclei and alteration in structure were observed for 0.1 mg/L. The findings of this histoarchitecture analysis of ovary indicate a direct correlation between cypermethrin exposure and the histoarchitecture disorders. Asian Australas. J. Biosci. Biotechnol. 2016, 1 (1), 47-53
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HALIM, K. M. ABDUL, MD NAWSHAD ALI, TANVIR RAHMAN, and MD ALI REZA FARUK. "Constraints of health management of commercially cultured high valued fishes." Bangladesh Journal of Fisheries 32, no. 2 (2021): 333–32. http://dx.doi.org/10.52168/bjf.2020.32.38.

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This study was performed to understand the constraints of health management of commerciallycultured high value fishes including gulsha (Mystus cavasius), shing (Heteropneustes fossilis) and koi (Anabastestudineus). One hundred farmers were interviewed through structured questionnaire in Mymensingh andJashore districts. Most of the farmers practiced polyculture of gulsha and shing with carps and monocultureof koi high densities. All the three species suffered from a number of serious diseases under farmingconditions. Most prevalent clinical signs of diseases were ulcerative hemorrhagic lesion, whitishappearance over body surface, ventral and mouth reddening and fin rot. The most susceptiblespecies to disease was shing with a higher average mortality of 43.33% followed by gulsha(36.71%) and koi (38.76%). Diagnosis of disease was the single most important constraints ofhealth management followed by lack of farmer’s knowledge on fish health and diseases, presence ofhuge number of poor quality medicine, ineffectiveness and indiscriminate use of such medicine,lack of farmer’s ability on application of medicine and absence of qualified fish health adviser.Lack of good quality fish seed, feed and water were identified as the main resource problemsassociated with fish health management. The study also identified a number of general constraintsof farming of high value fish which included low price of fish, higher feed cost, and outbreak ofdiseases, high land tax, electricity bill and marketing problem. Further studies should focus on thedevelopment of strategies for better health management practices for sustainable commercial cultureof high value fishes.
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Monir, Md Shirajum, Shuvho Chakra Borty, Nazneen Bagum, Md Khalilur Rahman, Md Alimul Islam, and Yahia Mahmud. "Identification of pathogenic bacteria isolated from diseased stinging catfish, Shing (Heteropneustes fossilis) cultured in greater Mymensingh, Bangladesh." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 1 (2016): 116–24. http://dx.doi.org/10.3329/aajbb.v1i1.61544.

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Stinging catfish, Shing (Heteropneustes fossilis) is been cultured in Bangladesh which is one of the most popular and highly valuable fish species. A total of 120 diseased H. fossilis were collected from twenty five fish farms in greater Mymensingh region, Bangladesh between November 2013 to February 2014. Remarkable clinical symptoms of the affected fishes were loss of equilibrium and, erosions at the bases of fins and tail, hemorrhages and skin lesions on body surface, profuse mucous secretion and congestion along with enlargement of most of the internal organs. Three different kinds of selective media were used for the isolation of total population of bacteria from the affected fishes. A number of biochemical tests were performed for the identification of the isolated bacteria. Commercially available panel of antibiotics were used for the determination of sensitivity of the isolated bacteria. A total of 85 bacterial strains were isolated and the isolated were Aeromonas hydrophila, A. salmonicida, A. sobria, Pseudomonas angulliseptica, P. fluorescens and Vibrio anguillarum. Aeromonas spp. appeared to be the main pathogen in the diseased fishes. Among the isolated spp of bacteria distribution of Aeromonas species was as follows: A. hydrophila 34 (40.00%), A. salmonicida 14 (16.47%), A. sobria 8 (9.41%) as well as 5 (5.88%) unidentified Aeromons strains. The other isolated of Pseudomonas anguliseptica, P. fluorescens, Vibrio anguillarum and Vibrio spp were distributed as 12 (14.11%), 4 (4.70%), 3 (3.52%) and 5 (5.88%), respectively in infected H. fossilis. However, results of antibiotic sensitivity test revealed that all the bacterial isolates of H. fossilis were found more or less resistant to all most all the antibiotics with the exception of ciprofloxacin and levofloxacinn which were found highly sensitivity against all the strains of Aeromonass pp. and other species of bacteria. Asian Australas. J. Biosci. Biotechnol. 2016, 1 (1), 116-124
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Kamruzzaman, Md Abdullah Al Mahamud, Abdul Alim, Md Shahadat Hossen, Md Ariful Islam, and Md Abul Mansur. "Study on heavy metal content of Oreochromis niloticus, Heteropneustes fossilis and Pangasius sutchi collected from pond and open water." Research in Agriculture Livestock and Fisheries 5, no. 1 (2018): 117–26. http://dx.doi.org/10.3329/ralf.v5i1.36560.

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Present studies were conducted on the study of heavy metal content of three popular freshwater fish, tilapia Oreochromis niloticus, shing, Heteropneustes fossilis and pangus, Pangasius sutchi collected from pond and open water in Mymensingh. All samples were evaluated by studying unwanted heavy metal concentrations. Cadmium (Cd) concentration (μg/g) of pond water O. niloticus, H. fossilis and P. sutchi was found to be 0.21±0.02, 0.20±0.01 and 0.22±0.02 respectively and open water was 0.28±0.03, 0.25±0.02 and 0.29±0.03, respectively. Copper (Cu) concentration (μg/g) of pond water O. niloticus, H. fossilis and P. sutchi was 0.25±0.02, 0.29±0.03 and 0.21±0.02 respectively whereas open water had 0.28±0.01, 0.59±0.04 and 0.35±0.01 respectively. Lead (Pb) concentration (μg/g) of pond water O. niloticus and H. fossilis contained 0.07±0.01 and 0.08±0.01, respectively whereas the open water had 0.15±0.02 and 0.15±0.01, respectively. Pb concentration both in pond and in open water P. sutchi was nil. Pb concentration of O. niloticus was higher in open water fishes (0.15±0.02 μg/g) than the fishes of pond water (0.07±0.01 μg/g). Heavy metal concentration of O. niloticus, H. fossilis and P. sutchi was within permissible limits except Cd of open water fishes. The result revealed that open water fishes have higher concentration of heavy metals in their muscle than the fishes of pond water. The results indicate that the open water environment is polluted by various chemical composition as well as various toxic heavy metal pollutants.Res. Agric., Livest. Fish.5(1): 117-126, April 2018
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BASAK, DOLA, FATEMA JAHAN, K. M. ABDUL HALIM, MD NAWSHAD ALI, and MD ALI REZA FARUK. "Biosecurity practices in hatcheries of high value fishes." Bangladesh Journal of Fisheries 34, no. 1 (2022): 9–17. http://dx.doi.org/10.52168/bjf.2022.34.2.

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The present study was conducted to know the status of biosecurity from some commercial fish hatcheries producing high value fish seeds including gulsha (Mystus cavasius), shing (Heteropneustes fossilis) and koi (Anabas testudineus) in Mymensingh and Jessore districts. Data was collected from 90 hatcheries of each district through questionnaire interview. Biosecurity status of hatcheries was evaluated through some previously set criteria including infrastructure, restriction on entry of visitors, use of foot bath, protective clothing, hatchery hygiene, disinfection and sanitation, record keeping, hatchery personnel’s academic qualification and training. Severe lacking on some biosecurity measures were observed such as use of foot bath, restriction on visitors, record keeping system, pest control management, proper disinfection of equipment, and feed inspection. No hatchery owners were found to use any protective clothing and vaccination. Some biosecurity measures were found quite satisfactory such as good hygiene (86%), cleaning of hatchery units (100%), water quality (78%), stocking of disease free broods (77.50%) and internal quarantine procedure (80%). Hatchery owners reported some diseases in brood fishes which included gill and fin rot, abdominal distension, ulcerative hemorrhagic lesion and whitish appurtenance. Overall the biosecurity status in hatcheries was not that satisfactory. It is thus important to improve biosecurity status in fish hatcheries through updated training of hatchery personnel.
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Patwary, ZP, MAR Faruk, and MM Ali. "Clinical and Histopathological Study of Important Air-Breathing Fishes." Progressive Agriculture 19, no. 1 (2013): 69–78. http://dx.doi.org/10.3329/pa.v19i1.17109.

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A study was conducted to know the health and disease problems of three important air-breathing fishes viz. Shing (Heteropneustes fossilis), Magur (Clarias batrachus) and Thai Koi (Anabas testudineus) through clinical and histopathological technique from June 2007 to March 2008 collected from selected farms and from local markets. Generally, during December and January, acute pathologies were recorded. Clinical signs of Shing included haemorrhage, extended belly and ulceration. Histopathologically, partly missing and splitted epidermis and dermis, necrotic, vacuoled and ruptured myotomes of muscle with fungal granuloma were observed. Major gill pathologies included partly missing and highly hypertrophied, haemorrhagic gill lamellae, presence of monogenetic trematode and pyknotic cells. In liver, haemorrhagic areas, necrotic, vacuoled, hyperplasid hepatocytes, cell debris, pyknotic nuclei and plenty of inflammatory cells were evident. Haemorrhages, vacuolation, necrosis, missing and ruptured kidney tubules and pyknotic nuclei were the major pathologies of kidney. Clinically, dark red lesion, haemorrhage, necrosis and ulcer in body surface were seen in Magur. Histopathologically observed pathologies in Magur were almost similar to that of Shing. Clinical signs of Thai Koi included discoloration, loss of scales and fins, abnormal caudal fin, haemorrhage in gill and ulcer. Marked histopathology in the skin and muscle were observed such as totally lost epidermis, dermis separated from muscle, severely ruptured, degenerated and missing of myotomes in many places. In gills, hypertrophy, hyperplasia, telangiectasis, clubbing, haemorrhage and massive necrosis in both primary and secondary gill lamellae were found. Pathologies observed in liver and kidney were most alike to that of Shing. In the months of February and March, all the investigated organs of the three fish species were at a healing stage.DOI: http://dx.doi.org/10.3329/pa.v19i1.17109 Progress. Agric. 19(1): 69 - 78, 2008
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Salam, S., KMS Rana, and MA Salam. "Comparative performances of exotic and indigenous fish species with commercial culture systems of Mymensingh district in Bangladesh." Progressive Agriculture 29, no. 4 (2019): 371–79. http://dx.doi.org/10.3329/pa.v29i4.41351.

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An experiment was conducted to assess the relative performances of indigenous and exotic fish species under commercial culture system at Muktagacha and Trishal upazila in Mymensingh district. Six exotic fish species namely Thai koi (Anabas testudineus), Thai pangus (Pangusius hypophthalmus), Silver carp (Hypophthalmichthys molitrix), Vietnam koi (Anabas testudineus), Big head carp (Aristichthys nobilis) and Tilapia (Oreochromis niloticus) and nine indigenous fish species namely Rui (Labeo rohita), Shing (Heteropneustes fossilis), Sar punti (Puntius sarana), Gulsha (Mystus bleekeri), Koi (Anabas testudineus), Mrigal (Cirrhinus cirrhosus), Kalibaush (Labeo calbasu), Catla (Catla catla) and Magur (Clarias batrachus) were selected in Muktagacha while exotic Big head carp and indigenous Gulsha, Koi and Magur were lacking for observation in Trishal upazila. Results revealed that the production of exotic Thai pangus was comparatively higher in Muktagacha (5810.81 kg/acre) than Trishal (4521.74 kg/acre) upazila. In contrast, silver carp and tilapia production were 3063.04 kg/acre and 1008.26 kg/acre in Trishal, respectively which were higher than Muktagacha. Average selling price of tilapia, Vietnam koi and silver carp was also comparatively higher in Muktagacha than Trishal upazila. Similar observation was also obtained in respect of indigenous fish species. On the other hand, average selling price of shing was found highest (BDT. 333.33 per kg) in Muktagacha than Trishal while other fish species were sold at similar price. Gross income was also significantly higher with the exotic fishes in Muktagacha accounted for BDT. 858,716/acre/cycle while the highest gross income obtained with the indigenous fishes was in Trishal upazila, valued BDT. 121,5987/acre/cycle. It can be concluded that, both indigenous and exotic fish farming has spread in the area because of easy to adopt culture system, availability of fry, feed, good profit and high demand in the market. Some limitations of the ongoing practices have been identified that need to be addressed properly by the concerned authority.&#x0D; Progressive Agriculture 29 (4): 371-379, 2018
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Ahmed, Md Benjir, M. Mamnur Rashid, Md Waheduzzaman, and Md Shafiul Alam. "Isolation and Identification of Aeromonas hydrophila from Carps, Catfishes, Perches, and an Eel from Mymensingh Region of Bangladesh." Journal of Agroforestry and Environment 17, no. 2 (2024): 12–15. http://dx.doi.org/10.55706/jae1711.

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Aeromonas hydrophila is a causative agent of MAS (Motile Aeromonas Septicemia) disease, causing huge mortality of fish all over the country. Isolation and identification of this bacteria, A. hydrophila from eight infected fish species: catla (Catla catla), rui (Labeo rohita), mrigal (Cirrhina cirrhosus), tarabaim (Macrognathus aculeatus), tilapia (Tilapia mossambicus), shing (Heteropneustes fossilis), koi (Anabas testudineus), and magur (Clarias batrachus) were undertaken from Tarakanda and Trishal upazila of Mymensingh District of Bangladesh. Fishes showed pale body color, external hemorrhages, reddish head, and reddish anal region. The intestine, kidney, and liver of each fish were inoculated over the AIM (Aeromonas Isolation Medium) and TSA (Tryptone Soya Agar) plates. The colonies of the first plates (AIM) were used for characterizing Aeromonas and the colonies of TSA plates were used for quantitative study. TSA plates showed the minimum bacterial load was 1.20 × 102 CFU/g in the kidney of tilapia and a maximum of 8.70 × 106 CFU/g in the intestine of catla. Isolated bacteria were finally identified as Aeromonas hydrophila by their physiological, biochemical, and morphological characteristics. They were rod-shaped, gram-negative, and motile. The bacteria showed positive reactions for catalase, oxidase, fermented glucose, and were resistant to vibriostatic agent 0129. A shortcut method of identifying A. hydrophila has been developed by this work. Thus, the bacteria as well as MAS disease can be detected easily, and our fish culturists will be able to save their cultured fish by using any recognized anti-Aeromonas drug.
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Jahan, Iftear, AKM Nur Alam Siddiki, M. Niamul Naser, and Md Abdus Salam. "Bioaccumulation and Toxicity of Iron Salt on Shingi Fish Heteropneustes fossils (Bloch) and its Possible Impacts on Human Health." Bangladesh Pharmaceutical Journal 18, no. 2 (2015): 179–82. http://dx.doi.org/10.3329/bpj.v18i2.24319.

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The freshwater Stinging catfish locally known as Shingi (Heteropneustes fossilis) is exposed to various concentrations of analytical grade FeSO4.7H2O. The LC50 values for Fe2+ were found to be 109, 68 and 45 mg/l at 24, 48 and 72 hrs, respectively indicating that the toxicity increased with time. Gills appear to be the first target organ for iron toxicity followed by the liver and muscle. Moisture contents in fish tissue increased due to release of the toxicant by biological way. Protein and lipid contents are decreased and ash content is increased with the increase of exposure time. In contrast, iron is relatively nontoxic to Shingi fish at low dose, but long time exposure has adverse effects in fish tissue. Thus, exposure to iron salt at toxic level resulted in accumulation of iron in fish tissue. Hence, the consumption of this type of contaminated fish might have detremental effect on human health. However, this needs extensive study to make any final conclusion.Bangladesh Pharmaceutical Journal 18(2): 179-182, 2015
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Sultana, Nahid, Md Juwel Rana, and Md Aslam Khan. "Analysis of Proximate Composition of Shingi Fish (Heteropneustes fossilis) Fed with Poultry Offal Protein-Rich Formulated Feed under Captive Conditions." Asian Journal of Biology 20, no. 11 (2024): 64–72. http://dx.doi.org/10.9734/ajob/2024/v20i11449.

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The experiment aimed to evaluate the effects of poultry offal protein-rich formulated feed on the proximate composition of shingi fish (Heteropneustes fossilis) using four treatments and three replications over 60 days. By using the method of AOAC, dry matter (DM), moisture, crude protein (CP), crude fat (CF), and total ash were measured to observe the composition of shingi fish after feeding formulated feed. According to the study, shingi fish fed four distinct poultry offal-formulated feeds—Diet-1 (30% FM + 0% POM), Diet-2 (20% FM + 10% POM), Diet-3 (10% FM + 20% POM), and Diet-4 (0% FM + 30% POM)—had their proximate compositions changed. During the first phase, shingi fingerlings' proximate composition was ascertained to be 80.15% moisture, 19.85% dry matter, 58.51% crude protein, 13.02% ash, and 10.21% crude fat. After culturing with different formulated diets, the mean values of moisture, dry matter, crude protein, total ash, and crude fat were found 78.54%, 21.48%, 60.47%, 13.14%, and 12.04%, respectively. The study found significant variations (p&lt;0.05) in proximate composition between fingerling Shingi and those cultured with poultry offal-formulated diets. Among the formulated feds, diets-4 displayed the highest amount of crude protein (62.12%) and the lowest ash contents (12.14%) while Diet 3 contained the highest amount of dry matter (22.89%), ash (12.41%), and lipid contents (13.98%). The study suggests that poultry offal prepared-fed could be a low-cost, locally accessible protein substitute for fish meals that would also help sustainably farmed fisheries to boost fish yield.
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Sarkar, M. J. A., and M. M. Rashid. "Pathogenicity of the bacterial isolate Aeromonas hydrophila to catfishes, carps and perch." Journal of the Bangladesh Agricultural University 10, no. 1 (2012): 157–61. http://dx.doi.org/10.3329/jbau.v10i1.12108.

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Pathogenicity of a bacterial isolate Aeromonas hydrophila recovered from naturally diseased shing fish was investigated against catfishes (Heteropneustes fossilis and Clarias batrachus), carps (Labeo rohita, Catla catla and Cirrhinus cirrhosus) and perch (Anabas testudineus) of average body weight of 20.4 g for H. fossilis, 25.6 g for C. Batrachus, 35.2 g for L. rohita, 25.7 g for C. catla, 30.5 g for C. cirrhosus and 20.3 g for A. testudineus. Two different doses viz. 6.7 × 106 and 6.7 × 105 CFU/fish were injected intramuscularly. Pathogenicity of A. hydrophila was confirmed at water temperature of 30°C by mortality of 60% to 100% of all the tested fishes within 2-11 days. Injected A. hydrophila was re-isolated from liver, kidney and intestine of all the tested fishes. The highest bacterial loads in catfishes were found to be 5.5 × 108 CFU/g in the liver of H. fossilis and 5.6 × 107 CFU/g in the intestine of C. batrachus. The lowest bacterial loads were found to be 2.2 × 102 CFU/g in the kidney of H. fossilis and 2.4 × 103 CFU/g in the liver of C. batrachus. The highest bacterial loads in carps were found to be 4.9 × 109 CFU/g in the liver of C. catla, 7.7 × 108 CFU/g in the intestine of L. rohita and 5.8 × 108 CFU/g in the intestine of C. cirrhosus. The lowest bacterial loads were found to be 2.7 × 104 CFU/g in the kidney of C. catla, 3.0 ×104 CFU/g in the kidney of L rohita and 5.6 × 103 CFU/g in the kidney of C. cirrhosus. The highest and lowest bacterial load in perch was found to be 6.4 × 10' CFU/g and 1.6 × 102 CFU/g in the intestine and kidney of A. testudineus respectively. In all the cases of intramuscular injection, external pathology was found. Reddish anal region and fm bases were observed. It was understood that the isolate was a highly virulent pathogen for the challenged fishes. DOI: http://dx.doi.org/10.3329/jbau.v10i1.12108 J. Bangladesh Agril. Univ. 10(1): 157–161, 2012
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Chakraborty, BK, and NN Nur. "Growth and Yield Performance of Chingi, Heteropneustes fossilis and Koi, Anabas testudineus in Bangladesh under Semi-Intensive Culture Systems." International Journal of Agricultural Research, Innovation and Technology 2, no. 2 (2013): 15–24. http://dx.doi.org/10.3329/ijarit.v2i2.14010.

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Production potential of shingi, Heteropneustes fossilis and koi, Anabas testudineus in polyculture were assessed at a stocking density of 2, 47,000 and 3, 70,500 ha-1, respectively in treatment T1 and T2. Monoculture of H. fossilis and A. testudineus was designed at a stocking density of 2, 47,000 ha-1 in treatment T3 and T4, respectively. Culture period of shingi was 120 days and koi was 100 days in all treatments. All fingerlings were of the same age at stocking, with a mean weight of 2.54±0.08 g for shingi and 0.50±0.01 g for koi, respectively. Commercial fish feeds (30.0% crude protein) supplied at the rate of 100 to 5% of total biomass twice daily. Fish production in treatment T1, T2, T3 and T4 were 18,803±111, 12,388±115, 10,042±5 kg ha-1 day-120 and 22,176±7 kg ha-1 day-100, respectively. The feed conversion ratio (FCR) was significantly (P&lt;0.05) lower in T4 than that of the other three treatments. The net financial benefits incurred from treatment T1, T2, T3 and T4 were Bangladeshi Taka 17,65,769; 6,691; 15,83,990 and 16,29,409 BDT ha-1, respectively. The mean differences of gross yields and net benefits among different treatments were significant (P&lt;0.05). The polyculture technology of shingi and koi, and monoculture technology of koi may help to meet the dietary needs and improve the socio-economic status of the people of Bangladesh.DOI: http://dx.doi.org/10.3329/ijarit.v2i2.14010 Int. J. Agril. Res. Innov. &amp; Tech. 2 (2): 15-24, December, 2012
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Begum, Aleya, Ahmed Ismail Mustafa, Md Nurul Amin, Tasrina Rabia Chowdhury, Shamshad Begum Quraishi, and Nasrin Banu. "Levels of heavy metals in tissues of shingi fish (Heteropneustes fossilis) from Buriganga River, Bangladesh." Environmental Monitoring and Assessment 185, no. 7 (2012): 5461–69. http://dx.doi.org/10.1007/s10661-012-2959-4.

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Begum, Aleya, Ahmed Ismail Mustafa, Md Nurul Amin, Nasrin Banu, and Tasrina Rabia Chowdhury. "Accumulation and histopathological effects of arsenic in tissues of shingi fish (Stinging Catfish) Heteropneustes fossilis (Bloch, 1794)." Journal of the Asiatic Society of Bangladesh, Science 39, no. 2 (2014): 221–30. http://dx.doi.org/10.3329/jasbs.v39i2.17861.

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A 60-day experiment was conducted to compare the accumulation and toxicological effects of arsenic in muscle, intestine and liver of shingi fish, H. fossilis (Bloch) after exposure to two concentrations (7.0 and 20.0 ppm) of arsenic trioxide. The highest/maximum level of accumulation of arsenic was observed in the liver whereas the lowest level of arsenic was found to accumulate in the muscle tissues at the end of exposure period. It is apparent from the study that the damage of the liver of test fish due to 15 days exposed period was less compared to the damage caused by 60 days exposure periods. The intensity of histological alterations was observed to increase gradually with the arsenic concentration and the exposure time. DOI: http://dx.doi.org/10.3329/jasbs.v39i2.17861 J. Asiat. Soc. Bangladesh, Sci. 39(2): 221-230, December 2013
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Subba, Reddy K.V, and G. Simhachalam. "Dietary Aqua Fix Effectively Alters the Qualitative Protein Profile in the Head Kidney." Biolife 10, no. 4 (2022): 16–21. https://doi.org/10.5281/zenodo.7286178.

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<strong>Abstract</strong> The qualitative protein profile in head kidney of 6, 9 and 12 months old <em>Labeo rohita</em> was investigated by feeding with a diet mixed with an immunomodulator, Aqua Fix. 6 (group A), 9 (group B), and 12 (group C) months old fish (12-15 g wt.; 12 fish in each group) were fed with a diet mixed with Aqua Fix @ 50 mg/100 g of feed for 4 days.&nbsp; Another 3 groups (a, 6 months; b, 9 months; and c, 12 months) of same weight (12 fish in each group) were fed with a normal diet to act as controls for comparison.&nbsp; Head kidney showed significant alteration (with low and high molecular weight proteins) in the qualitative protein profile (analyzed by SDS page analysis), on day 1, 4, 7, 15 and 30 of experiment in all the three groups of 6 (group A), 9 (group B) and 12 (group c) months old fish.&nbsp; These results determine the effect of Aqua Fix in modulating the immune system of fish with regard to the marked changes in the profile of proteins. <strong>Keywords:</strong> Rohu<em>.</em> Aqua Fix, head kidney, qualitative proteins <strong>References</strong> Ashraf, M., Ayub, M. and Rauf, A. 2008. Effect of different feed ingredients and low temperature on diet acceptability, growth and survival of <em>Mrigal, Cirrhinus mrigala </em>fingerlings. Pakistan J. Zool. 40(2): 83-90. Delbarre-Ladrat, C., Cheret, R., Taylor, R. and Verrez-Bagnis, V. 2006. Trends in postmortem aging in fish: understanding of proteolysis and disorganization of the myofibrillar structure. Crit. Rev. Food Sci. Nutr. 46: 409-421. Demir, O., Gulle, I., Gumos, E., Kucuk, F., Gunlu, A. and Kepenek, K. 2010. Some reproductivefeatures of brown trout (Salmo truta macrostigma Dumeril, 1858) and its larval development under culture conditions. <em>Pak. Vet. J</em>. 30(4): 223-226. Demir, O., Gunlu,A., Kucuk, F., Gulle, I. and Gumus, E. 2011. Analysis of sarcoplasmic proteins in natural populations of mountain trout (<em>Salmo trutta macrostigma </em>Dumeril, 1858) with SDS-PAGE. Afri. J. Biotechnol. 10(55): 11758-11763. Fock, U. and Hinssen, H. 2002. Nebulin is a thin filament protein of the cardiac muscle of the agnathans. J. Muscle Research and Cell Motility. 23(3): 205-213. Gangwar, G., Rajesh Singh, Rao, A.P. and Tewari, S.C. 2007.Changes in biochemical composition of muscles of Indian major carp <em>Labeo rohita</em> (Ham.) in influence of age. The Asian J. Ani. Sci. 2(1 and 2): 46-48. Geri, G. Poli, B.M., Gualtieri, M.,Lupi, P. and Parisi, G. 1995.Body traits and chemical composition of muscles in the common carp (<em>Cyprinus carpio</em>) as influenced by age and rearing environment. Aquaculture, 129: 329-330. Gomez Guillen, M.C., Montero, P., Hurtado, O. and Borderias, A. 2000. Biological characteristics affect the quality of farmed Atlantic salmon and smoked muscle. J. Food Sci. 65(1): 53-60. Islam, M.T., Rashid, M.M. and Mostafa, K. 2008. Histopathological studies of experimentally infected shing, <em>Heteropneustes fossilis </em>with <em>Aeromonas hydrophila </em>bacteria. Progress. Agric. 19(1): 89-96. Jitender Kumar, J., Pal, K., Devivaraprasad Reddy, A., Sahu, N.P., Venkateshwarlu, G. and Vardia, H.K. (2012). Fatty acids composition of some selected Indian fishes. <em>African J. Basic Appl. Sci</em>. 4(5): 155-160 Ladrat, C., Chaplet, M., Verrez-Bagnis, V., Noel, J. and Fleurence, J. 2000. Neutral calcium-activated proteases from European sea bass (<em>Dicentrarchus labrax </em>L.) muscle: Polymorphism Biochem. Stud. Comp. Biochem. Physiol. 125B: 83-95. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophase T4 Nature. 227: 680-685. Li, M.H., Bosworth, B.G. and Robinsson, E.H. 2000. Effect of dietary protein concentration on growth and processing yield of channel catfish (<em>Ictalurus punctatus</em>). J. World Aquaculture. Soc. 31: 592-598. Magnodottir, B. 2010. Immunological control of fish diseases. J. Mar. Biotechnol. 12: 361-379. Mahaboob, S., Farooq, M., Mahmood, D., Nasir, N., Sultana, S., Choudhry, A.S., Al-Akel, A.S., Al-Balawi, H.F.A., Al-Misned, F. and Al-Ghanim, K.A. (2012). Phylogenetic relationship of cultured and wild <em>Labeo rohita</em> and <em>Cirrhinus mrigala</em> based on muscle proteins profile in different weight groups. A new tool in phylogenetic analysis. <em>Int. J. Food. Prop</em>. 25: 949-960. Manoj, C.K., Mohankumaran, Nair, C., Patel, M.B. and Salin, K.R. 2010.Haematobiochemical and histopathological changes in <em>Labeo rohita</em> infected with <em>Aeromonas hydrophila</em> by immersion challenge. Fishery Technol. 47(2): 151-160. Mathew, S. and Prakash, V. 2006.Effect of calcium salts on the properties of proteins from oil sardine (<em>Sardinella longiceps</em>) during frozen storage. J. Food Sci., 71: 178-183. Mitsuhashi, T., Kasai, M. and Hatae, K. 2002. Detection of giant myofibrillar proteins connection and nebulin in fish meat by electrophoresis in 3-5 gradient sodium dodecyl sulfate polyacrylamide slab gels. J. Agric. Food Chem., 50(26): 7499-7503. Montowska, M. and Pospiech, E. 2007.Species identification of meat by electrophoretic methods. Acta Sci. Pol. Technol. Aliment., 6(1): 5-16. Mostofa, K., Islam, M.T., Sabur, M.A. and Rashid, M.M. 2008. Experimental pathogenesis of <em>Aeromonas hydrophila</em> bacterium in stinging catfish <em>Heteropneustes fossilis</em>. Bangladesh J. Fish. Res., 12(1): 27-33. Okagaki, T., Takami, M., Hosokawa, K., Yano, M., Fujime, S.H. and Ooi, A. 2005. Biochemical properties of ordinary and dark muscle myosine from carp skeletal muscle. J. Biochem., 138: 255-262. Plumb, J.A. and Hanson, L.A. 2011. Health maintenance and principal microbial diseases of cultured fishes. 3<sup>rd</sup> Edition, Wiley, Blackwell, Ames. Press, C. and Evensen, M.c.L.ϕ. 1999. The morphology of the immune system in teleost Fishes. Fish &amp; Shellfish Immunology. 9: 309-318. Rahman, M.H., Suzuki, S. and Kawai, K. 2001.The effect of temperature on <em>Aeromonas hydrophila</em> infection in gold fish, <em>Carassius auratus,</em> J. Appl. Icthyol. 17: 282-285. Sahoo, P.K. and Mukherjee, S.C. 2003. Immunomodulation by dietary vitamin C in healthy and aflatoxin B1-induced immunocompromised rohu (<em>Labeo rohita</em>). Comp. Immunol. Microbiol. Infect. Dis. 26: 65-76. Sahoo, P.K. and Mukherjee, S.C. 2002.The effect of dietary immunomodulation upon <em>Edwardsiella tarda</em> vaccination in healthy and immunocompromised Indian major carp (<em>Labeo rohita</em>). Fish &amp; Shellfish Immunol. 12: 1-16. Sahoo, P.K., Kumari, J. and Mishra, B.K. 2005. Non-specific immune responses in juveniles of Indian major carps. J. Apl. Ichtyol. 21: 151-155. Salim, M. (2006). Role of fish as food to human nutrition. International Conference on &ldquo;Solving Problems of Fresh Water Fish Farming in Pakistan&rdquo;, p.20. November 27-28, UVAS, Lahore, Pakistan. Sarkar, M.G.A., Chowdhury, B.M.R., Faruk, M.A.R. Uddin, M.N. and Islam, M.J. 2000. Effect of water temperature on the infectivity of <em>Aeromonas hydrophila</em> isolates. Bangladesh J. Fish. 23(2): 99-105. Sultana, S., Zahra, A., Sultana, T., Al-Ghanim, K.A. and Mahboob, S. 2016.Effect of different artificial feeds formulated from local ingredients on the meat quality of Indian major carps. The J. Animal and Plant. Sci. 26(4): 1140-1145. Tanriverdi, E.A., Yilmaz, M., Koc, E., Akgul, A. and Ersan, Y. 2016.The effect of dimethoate on <em>Capoeta capoeta </em>(Guldenstaedt 1773) and the protective effect of ascorbic acid.Fres. Env. Bul. 25: 5974-5979. Vidyasagar Reddy, G. and Vijaya, Ch. (2014). Protein profile of selected commercial freshwater fishes of Nellore, India, <em>Biotechnol</em>. 3: 1-3.
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Shukla, Madhulekha, and Sunita Arya. "CADMIUM TOXICITY INDUCED MORPHOLOGICAL ALTERATION IN INDIGENOUS FISH Heteropneustes fossilis (Bloch.)." Green Chemistry & Technology Letters 3, no. 1 (2017): 21–25. http://dx.doi.org/10.18510/gctl.2017.315.

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The aim of present study was to determine the toxicity (LC50) of cadmium chloride in freshwater catfish Heteropneustes fossilis. Acute toxicity of cadmium on the indigenous fish H., fossilis was designed in the fish aquarium in laboratory at room temperature in Department of Zoology, DGPG College at Kanpur.Treated fish H., fossilis induced morphological alteration against cadmium chloride toxicity. H. fossilis showed morphological alteration such as increased opercula movement, abnormal swimming, and loss of buoyancy and fading of their body colour. Control fishes were also continuously monitored and compared with the changes caused by cadmium concentration. Heteropneustes fossilis exposed different concentration of cadmium chloride toxicity i.e. 0 ppm for 24 h, 10 ppm for 48h, 15ppm for 72h and 20 ppm for 96 h. From this present study it seems that the indigenous fish, H. fossilis is more susceptible to cadmium toxicity.
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30

Ali, A., T. Akter, and M. Das. "Use of Waste Energy by Adding Shing (Heteropneustes fossilis, Bloch 1797) into Pangus (Pangasianodon hypophthalmus, Sauvage, 1878) Pond." Journal of Environmental Science and Natural Resources 6, no. 1 (2015): 209–16. http://dx.doi.org/10.3329/jesnr.v6i1.22067.

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An experiment was conducted to evaluate the growth, survival and production performances of pangus (Pangasianodon hypophthalmus) and shing (Heteropneustes fossilis) in which shing was used as guest to utilize the waste energy in pangus pond. The experiment was run for four months from July to October, 2008. Fishes were reared in pond no I, II, III with stocking density 150, 250 and 300 per decimal of pangus respectively. Shing was stocked only 10/decimal in pond-I. A complete artificial feed was used containing 23% protein. The weight gain (385.17±2.15g) of pangus was significantly higher in pond-I than that of pond-II (325.15±2.70g) and pond-III (319.30±2.90g). However, the production of pangus per ha were (28158.00±0.10kg) in pond-I, (34159.112±0.50 kg) in pond-II and (37856.208±0.60 kg) in pond-III. Total production of shing in pond-I was 240.57±1.60 kg/ha/yr. The survival rates (%) of pangus under three stocking densities were 98.66%, 85.06% and 80.00% respectively. The food conversion ratio (FCR) of pangasius pond I, II and III were 1.53, 1.81 and 1.84 respectively. The lowest FCR of 1.53 was found in pond-I. The net return of the pond I, II and III were BDT 273503.10, 117456.73 and 107906.60 per ha per year. The addition of shing into pangasius pond as guest invites economic benefit to the pangus culture.DOI: http://dx.doi.org/10.3329/jesnr.v6i1.22067 J. Environ. Sci. &amp; Natural Resources, 6(1): 209-216 2013
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Md., Munsur Ali, Abdulla-Al-Asif, Azharul Islam Shabuj Md., et al. "Dose optimization with synthetic hormone flash for induced spawning of Shing (Heteropneustes fossilis)." International Journal of Fauna and Biological Studies 3, no. 1 (2015): 39–45. https://doi.org/10.5281/zenodo.5043420.

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An experiment on induced breeding of Heteropneustes fossilis was carried out to determine the optimum dose of Flash hormone at the Bogura Matshya Khamar, Parulia, Palash, Norshindhi. The present study consisted of three treatments (T1, T2 and T3) with nine replications of each. Forty five pairs of male and female were selected from the brood rearing ponds and the average body weight of the female and male were 100&plusmn;10 g and 55&plusmn;5 g respectively. To observe the effective dose forinduced breeding, the females were injected at the rate of 0.5 (T1), 0.45 (T2) and 0.42 (T3) ml Flash/kg body weight and correspondingly the males were administered a dose of 0.20 (T1), 0.18 (T2) and 0.17 (T3) ml Flash/kg body weight in all treatments. The fertilization rate and hatching rate were determined. Then the hatchlings were reared in aquarium up to 7 days and survival rate was determined. The water temperature was recorded between 26 to 310C during experimentation. Among the three treatments T1 showed the best result in terms of fertilization rate (80.33%), hatching rate (71.67%) and survival rate (61.56%). The present findings can be used in induced breeding of Heteropneustes fossilis for the development of hatchery propagation.
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32

Khalil, Sarker M. Ibrahim, Kirtunia Juran Chandra, and David Rintu Das. "Effects of Endoparasitism of Heteropneustes fossilis on condition factor, gonad and blood composition of host." Research in Agriculture Livestock and Fisheries 1, no. 1 (2015): 159–67. http://dx.doi.org/10.3329/ralf.v1i1.22379.

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Effects of endoparasitism of Heteropneustes fossilis on condition factor, gonad and blood composition of the host was conducted during July 2012 to June 2013. Fish samples were collected from various water bodies of Sylhet region. Total length, body weight, gonad weight and sex of hosts were recorded. Blood sample was collected from each fish. Six different species of parasites were identified from the hosts assessed as i). Euclinostomum multicaecum, ii) Allocreadium handiai, iii). Lytocestus indicus, iv). Pseudocaryophyllaeus heteropneustus, v). Procamallanus heteropneustus and vi). Paracamallanus equispiculus. Gonad weight, condition factors and blood parameters of H. fossilis were greatly affected with moderate infestations. The highest condition factor, 0.59, was found in uninfested fish and lowest, 0.45, in infested fish. Changes in the percent of haemoglobin and erythrocyte sedimentation rate (ESR) was also investigated. Percent loss of haemoglobin was 0.43 and erythrocyte sedimentation rate (ESR) was 5.19 mm/h. In general the health condition parameter due to effects of endoparasitism of the investigated fish was found negative impact for the culture production of the Singhi (Heteropneustes fossilis). DOI: http://dx.doi.org/10.3329/ralf.v1i1.22379 Res. Agric., Livest. Fish.1(1): 159-167, Dec 2014
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33

Mishra, Sachchidanand. "A histological observation on heavy metal induced implications on respiratory epithelium and its causative impact on a catfish Heteropneustes fossilis (Bloch)." International Journal of Zoology and Applied Biosciences 9, no. 6 (2024): 29–32. http://dx.doi.org/10.55126/ijzab.2024.v09.i06.005.

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Present study is based on lead nitrate induced histological effect on the respiratory organs of Heteropneustes fossilis (Bloch). Lead nitrate is a heavy metal compound which used in many industries and indiscriminately drains out in water system and contaminates aquatic medium leads serious hazardous effect on respiratory epithelium of fish. Lead nitrate induced alteration on respiratory epithelium associated with the air breathing organ, suprabranchial chamber and air sacs of Heteropneustes fossilis (Bloch) and also affect the oxygen consumption capacity of fish which adversely affect its survival. Heavy metal poisoning produced remarkable histopathological deleterious effect on the accessory respiratory organ decreases the rate of oxygen consumption. Various concentration of lead nitrate showed noticeable histopathological changes which is a sign of serious effect of this heavy metal and its bioaccumulation ultimately dangerous for human consumption of contaminated fish through food chain.
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34

Zafar, Abu. "Comparative growth performance assessment of Shing(Heteropneustes fossilis)feeding with prepared and commercial diet." Journal of Aquaculture & Marine Biology 9, no. 1 (2020): 10–13. http://dx.doi.org/10.15406/jamb.2020.09.00270.

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Prakash, Chandra, and Sunil Kumar Srivastav. "Microcystin-LR: Effects on Freshwater Catfish Heteropneustes fossilis Prolactin Cells." Iranian Journal of Toxicology 13, no. 4 (2019): 17–20. http://dx.doi.org/10.32598/ijt.13.4.562.1.

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Background: Previous studies have been reported on the toxicity of Microcystin-LR, which is produced by cyanobacterial growth in fish, such as Heteropneustes fossilis (H. fossilis). However, no studies have been conducted on the effects of Microcystin-LR on the prolactin cells of H. fossilis. Methods: H. fossilis fish were intraperitoneally injected with Microcystin-LR (2.5μg/25g) and sacrificed after 1, 3, 5, 10 and 15 days. The blood samples were analyzed for the calcium levels. Histological slides of the pituitary prolactin cells were stained with Herlant’s tetrachrome and Heidenhan’s azan techniques, and examined under light microscopy. Results: The prolactin cells exposed to Microcystin-LR exhibited no structural changes on day 1. However, hyperactive prolactin cells exhibited cellular degranulation from day 3 to day 5. On days 10 and 15, degenerated and vacuolated prolactin cells were also observed. The nuclear volume of prolactin cells exposed to MC-LR increased progressively from day 3 to day 10 but on day 15 the nuclear volume returned to normal. The serum calcium level of MC-LR injected specimens showed hypocalcemia from day 3 to day 10. On day 15, the level became normal. Conclusions: Our results indicated that the serum calcium levels and prolactin cells were altered after exposure to microcystin-LR.
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Prasad, Nayan K., Kumari Shachi, and Suresh Kumar Sahani. "TOXICOLOGICAL ASSESSMENT OF COPPER SULPHATE ON CHANNA ORIENTALIS AND HETEROPNEUSTES FOSSILIS: LC50 VALUES AND MORTALITY ANALYSIS." European Journal of Medical Genetics and Clinical Biology 1, no. 7 (2024): 20–26. http://dx.doi.org/10.61796/jmgcb.v1i7.701.

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The adverse effects of heavy metals, particularly copper sulphate, released from industrial effluents on aquatic life are well-documented. In this study, we determined the LC50 values of copper sulphate for two freshwater air-breathing fish species, Channa orientalis (Bloch) and Heteropneustes fossilis (Bloch). The LC50 values for Channa orientalis were determined to be 33 ppm at 24 hours, 32 ppm at 48 hours, 21 ppm at 72 hours, and 7.5 ppm at 96 hours. For Heteropneustes fossilis, the corresponding LC50 values were 15 ppm at 24 hours, 13 ppm at 48 hours, 9.5 ppm at 72 hours, and 5 ppm at 96 hours. We observed a significant increase in mucus secretion at higher concentrations of copper sulphate as compared to lower concentrations. The potential mechanisms of fish mortality under these conditions are also discussed in detail
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37

Neowajh, Md Sharif, M. Mamnur Rashid, Abdulla Al Asif, Md Abu Zafar, and Amir Hossain. "Effects of chemotherapeutics against experimentally injured stinging catfish Heteropneustes fossilis." Asian Journal of Medical and Biological Research 3, no. 4 (2018): 476–87. http://dx.doi.org/10.3329/ajmbr.v3i4.35338.

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Effects of different chemotherapeutics were examined against experimentally infected stinging catfish Heteropneustes fossilis. Fish were collected from a fish market in Mymensingh, acclimatized for 7 days in laboratory condition from January to February, 2016 in aquaria at Fish Clinic of Bangladesh Agricultural University, Mymensingh, experimentally injured by using forceps and knife and waited seven days for the establishment of infection. Before starting chemotherapeutic trial, it was confirmed that the fish were infected with microorganisms by clinical diagnosis in laboratory condition. They showed hemorrhages and ulcerative lesions over the infected area. A total of 80 such experimentally infected stinging catfish having average body weight of 18 g were used for the experiment. Two chemotherapeutics: antibiotic, Eryvet (erythromycin thiocyanate INN, sulphadiazine (NaUSP) &amp; trimithoprim BP) and antifungal, methylene blue were used in separate and combined treatment with three different doses of antibiotic. The same dose of methylene blue, 0.2 mg/l, was used for separate and combined treatment by antibiotic. Doses of antibiotic (Eryvet) were 0.8 g/10 kg body weight of fish, 1g/10 kg body weight of fish and 1.2 g/10 kg body weight of fish as lower dose, recommended dose and higher dose respectively. For combined treatment the above different doses of antibiotic and the same dose of antifungal were used. Water was exchanged regularly. The chemotherapeutic trial was conducted for 7 days and observation was continued for another 8 days to observe the effect of treatment. Combined treatment with the recommended dose of the antibiotic and methylene blue showed the best result where 90% fish were recovered. By the treatment with the higher dose of the antibiotic 70% fish were recovered. Antifungal treatment showed that 20% fish were recovered. All the fish in negative control aquarium died.Asian J. Med. Biol. Res. December 2017, 3(4): 476-487
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38

Prasanta, Nanda, and Bikkini Ajaya. "Haematological changes in fish Heteropneustes fossilis under vanadium stress." Biolife 4, no. 4 (2022): 695–96. https://doi.org/10.5281/zenodo.7350583.

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<strong>ABSTRACT</strong> Vanadium toxicity to fish <em>Heteropneustes fossilis</em> (Bloch) was studied for a period of two weeks. Haematological parameter like Total Erythrocyte Count (TEC), Haemoglobin (Hb) and Total Leucocyte Count (TLC)&nbsp; altered&nbsp; significantly&nbsp; ( P &lt; 0.001 ) in fish when&nbsp; exposed&nbsp; to 1,4, and 7 ppm of Vanadium (NaVO<sub>3</sub>). Absolute value like MCH changed in accordance with the above parameter. <strong>Key Words:</strong> &nbsp;Seasonal changes, <em>Chiloscyphus gollani, Solenostoma crenulata</em>,<em> Fossombroni&nbsp; himalayensis</em>, inverse relation. <strong>REFERENCES</strong> Bannerjee V and Banerjee M .1988effect of heavy metal poisioning on peripheral haemogram in <em>Heteropneustes fossilis (Bloch)</em> mercury,chromium and zinc chloride (Lc<sub>50</sub>),comp. physiol ,ecol,13(2), 128-134 Porika Raju and Estari Mamidala (2015). Anti-diabetic activity of compound isolated from Physalis angulata fruit extracts in alloxan induced diabetic rats. The Ame J Sci &amp; Med Res, 2015,1(1); Pages 1 -6. doi:10.17812/ajsmr2015.11.1 Cheng I.W., M. A Albayati., M .R. Culbertson , L S Rosblatt &amp; L.D. Hansen 1982.Acute toxicity of&nbsp; Ammonium metavanadate in mice, J.Toxicol &amp; Environ&nbsp; Health,10 : 673-687. Dacie J.V and S.M.Lewis 1975. Practical haematology , J &amp; A Churchill ltd, London, UK Gummow B. 2011. Vanadium: Environmental Pollution &amp; Health effects. Elsevier B.V, 628-636. Gupta S. &amp; V Rai.1995.Effect of Vanadium on phosphatases and transaminases in certain organs of&nbsp;&nbsp;&nbsp; <em>Clarias batrachus</em> , 22,71-77 Nanda, P. and M.K. Behera 1996 .Nickel induced changes in some haematobiochemical parameters of a cat fish <em>Heteropneustes fossilis</em> (Bloch). Env.Ecol. 14 :82-85.
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39

Md., Munsur Ali, Abdulla-Al-Asif, Azharul Islam Shabuj Md., et al. "Technology of artificial breeding of catfish species in the hatcheries in Jessore Region, Bangladesh." International Journal of Fisheries and Aquatic Studies 4, no. 1 (2015): 180–88. https://doi.org/10.5281/zenodo.5043259.

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The present study was carried out to know the artificial breeding of Ompk pabda, Heteropneustes fossilis and Pangasius hypophthalmus using pituitary gland (PG) from June to August 2014, in the hatcheries in Jessore region. Brood stock of Pangus, Shing, and Pabda was developed in the hatchery. A total of 10 hatcheries were included in the study. Brood fishes were reared in the brood rearing pond by providing artificial diet for good health and full maturation. Brood fishes were injected with PG in different months June to August. Both male and female were injected with PG. In Pabda, Shing and Pangus first PG dose was applied at 8-10, 30-35 and 1-2 mg PG/kg of body weight of females respectively. After 1 st PG dose of Pabda, Shing, and Pangus (6 hours later) female were treated with 2nd doses at 10-12, 60, 8-10 mg PG/kg of body weight, respectively. At the time of second dose of female, the males were also treated with single PG dose. The males of Pabda, Shing and Pangus were treated with 8-10, 30-35 and 1-2 mg PG/kg body weight respectively. It was observed that the female and male brood fishes eject eggs and milt within 12-14 hours respectively. Ovulation occurred in Pabda, Shing, and Pangus naturally after, 6-7 hours. The fertilized eggs of Pabda, Shing and Pangus were hatched within 22-24 hours. The temperature, pH, dissolved oxygen and transparency ranged from 22-34 0 C, 6-8.5, 5-7.5 ppm and 22-32 cm of the water of brood rearing ponds respectively. The average spawn production and body weight of female Pabda, Shing and Pangus were 11500/kg and 120/gm, 21000/kg and 130/gm, 44500/kg and 3.5/kg, respectively. The average fertilization, hatching and survival rate of Pangus (80%, 73% and 64%), Shing (73%, 68% and 58%) and Pabda (78%, 65% and 60%) respectively. The male and female ratio was maintained as 1:2 for each fishes.
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40

Bhuiyan, Md Aminul Islam, Zannatun Nahar Jhinu, Rita Parveen, and Abdul Jabber Hawlader. "Community structure of protozoan parasites in Heteropneustes fossilis (Bloch 1794) in Bangladesh." Dhaka University Journal of Biological Sciences 30, no. 2 (2021): 307–16. http://dx.doi.org/10.3329/dujbs.v30i2.54655.

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The study was conducted to prepare a database for the infection status of protozoan parasites on an important host fish species of Bangladesh, Heretropneustes fossilis. Host samples were collected from the freshwater habitats of six different districts of Bangladesh- Manikganj, Faridpur, Mymensingh, Kishoreganj, Bogura, and Jashore. H. fossilis was noted to be infected by 6 parasite species, of which 3 belonged to myxozoa (Henneguya singhi, Henneguya qadrii and Henneguya mystusia); 1 belonged to ciliophora (Trichodina siddiquae) and 2 (Trypanosoma singhii and Piscinoodium pillulare) belonged to mastigophora.The parasites, Trypanosoma singhii and Henneguya singhi were recorded as new locality record in H. fossilis. The three parasites Piscinoodium pillulare, Henneguya qadrii and Henneguya mystusia were the first recorded parasites in this fish and the first locality record in Bangladesh. The parasites were observed to occupy gill, body slime, and blood. Gill parasites were abundantly found compared to body slime and blood parasites. The highest prevalence (67.21%) of infection of H. fossilis was observed in Manikganj and the lowest prevalence (54.67%) of infection was observed in Bogura. Parasites of H. fossilis showed the highest diversity in fishes of Faridpur (2.63). Species richness of parasites was highest in Manikganj (0.38) and species evenness was relatively low (0.13-0.19) in almost all the study sites.&#x0D; Dhaka Univ. J. Biol. Sci. 30(2): 307-316, 2021 (July)
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Sachchu, Md Mazedul Haque, Amir Hossain, Md Mahmudul Kobir, et al. "Heavy Metal Intake by Fishes of Different River Locations in Bangladesh: A Comparative Statistical Review." Asian Journal of Fisheries and Aquatic Research 26, no. 6 (2024): 43–67. http://dx.doi.org/10.9734/ajfar/2024/v26i6775.

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Bangladesh a nation with a high population density, gets all of the protein it needs from river fishes. The observation is very alarming that heavy metals polluted the major river water by the growing garment washing, dyeing and textile, fertilizer, cement, tannery, metal, electrochemical and pharmaceutical industries were the main culprits that don’t have proper effective effluent treatment plants (ETP). High-value heavy metals in industrial area river fish as chromium (Cr) 164.73 mg/kg in Heteropneustes fossilis (stinging catfish) at Buriganga River, Hazaribagh, zinc (Zn) 309.47 mg/kg in Mastacembelus armatus (Bain) fish at Bangshi river, Savar, copper (Cu) 26.33 mg/kg in Mastacembelus armatus (Bain) at Bangshi river, Savar, arsenic (As) 5.64 mg/kg in Heteropneustes fossilis (stinging catfish) at Bangshi river, Savar, lead (Pb) 18.16 mg/kg in Channa punctatus (Taki) at Buriganga river, Hazaribagh and cadmium (Cd) 2.03 mg/kg in Heteropneustes fossilis (stinging catfish) at Buriganga river, Hazaribagh were observed. The two rivers are identified as most contaminated by heavy metals one is the Bangshi River, Savar, Gazipur and another one Buriganga River, Hazaribagh. The main reason for the contamination of these two rivers was uncontrolled industrialization around Dhaka and in Hazaribagh, a huge number of tanneries have grown that use a huge quantity of Cr creating a threat to the ecosystem and even a serious health risk for humans. Heavy metals that fish consume are contaminating river water ecosystems. This contamination spreads to human bodies and is the cause of many serious ailments.
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42

Dr., Bheem Rao, Ramana Rao. A. T, and P. Jyothi. "A Comparative Study of Anti Bacterial Activity of Epidermal Secretion of Two Fishes Channa punctatus and H.fossils (Bloch)." A Comparative Study of Anti Bacterial Activity of Epidermal Secretion of Two Fishes Channa punctatus and H.fossils (Bloch) 8, no. 11 (2023): 5. https://doi.org/10.5281/zenodo.10154050.

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The present study carried out to determine the comparative study of antibacterial activity of epidermal secreations of two fishes channa punctatus and Hetetropneustes fossilis, against bacterial strains. i.e Escherchia coli, Klebsiella pneumonia, Staphylococcus aereus, Proteus vulgaris. In Channa punctatus higest Zone of inhibition occurs with Staphylococcus aureus (30mm) and minimum ZOI occurs with Klebsiella pneumoniae (19mm) . In heteropneustes fossilis the higest Zone of inhibition occurs Escherchia coli (30mm)., minimum ZOI with Klebsiella pneumonia (20mm) . The antibacterial activity of epidermal mucus secretion was compared between two fishes channa punctatus and Hetetropneustes fossilis .Keywords:- Antibacterial, fish mucus, Channa punctatus, Hateropneustes fossilis Epidermal mucus Pathogen, Zone of inhibition.
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43

Saha, Suchismita, and Anilava Kaviraj. "Acute Toxicity of Synthetic Pyrethroid Cypermethrin to Freshwater Catfish Heteropneustes fossilis (Bloch)." International Journal of Toxicology 22, no. 4 (2003): 325–28. http://dx.doi.org/10.1080/10915810305122.

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Lethal concentrations of cypermethrin, dissolved either in water or acetone, were determined for freshwater catfish Heteropneustes fossilis at different hours of exposure by static bioassays. Up to 48 hours, there was no difference between LC50 values of aqueous and acetone solublized cypermethrin. Seventy-two-hour LC50 values of aqueous cypermethrin and acetone solublized cypermethrin to H. fossilis were 0.67 and 1.27 μg/L, respectively. Lethal values remained unchanged beyond 72 hours. The fish exposed to even lower concentration of cypermethrin (0.5 μg/L) showed hyperactivity.
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44

Shanker, Rishi, and Prahlad K. Seth. "Toxic effects of acrylamide in a freshwater fish,Heteropneustes fossilis." Bulletin of Environmental Contamination and Toxicology 37, no. 1 (1986): 274–80. http://dx.doi.org/10.1007/bf01607761.

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45

Kumar, S., S. Lata, and K. Gopal. "Deltamethrin Induced Physiological Changes in Freshwater Cat Fish Heteropneustes fossilis." Bulletin of Environmental Contamination and Toxicology 62, no. 3 (1999): 254–58. http://dx.doi.org/10.1007/s001289900867.

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Dr. Molina Bindal. "Alteration in Haematological Parameters of Heteropneustes fossilis Exposed to Sodium Fluoride." Knowledgeable Research: A Multidisciplinary Journal 2, no. 05 (2023): 71–75. http://dx.doi.org/10.57067/y4twqs29.

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This Study examines how sodium fluoride influences the hematological characteristics of Heteropneustes fossilis when subjected to sub-lethal doses. The fish were treated with sodium fluoride concentrations of 2.20 ppm (equivalent to 1/6 of the 96-hour LC50) and 4.40 ppm (corresponding to 1/3 of the 96-hour LC50) over periods of 15 and 30 days, respectively. The outcomes revealed noteworthy changes in various parameters, including total erythrocyte count, haemoglobin concentration, haematocrit measurements, mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV), among others. These results indicate that extended exposure to sodium fluoride adversely affects the haematological well-being of H. fossilis.
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Saha, Shubhajit, Dip Mukherjee, Kishore Dhara, and Nimai Chandra Saha. "Acute Toxicity Bioassay of a Pyrethroid Pesticide Bifenthrin to the Asian stinging Catfish, Heteropneustes Fossilis (Bloch)." Current World Environment 16, no. 1 (2021): 250–58. http://dx.doi.org/10.12944/cwe.16.1.25.

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Bifenthrin is a type-I synthetic neopyrethroid pesticide having eight different stereoisomers. The current study aims to assess the short-term toxic effect of Bifenthrin to freshwater fish, Heteropneustes fossilis. The 24, 48, 72 and 96h LC50 values of bifenthrin to H. fossilis were 4.82, 4.47, 3.54 and 3.40 µg/l respectively. There was a significant variation (p&lt;0.05) in the mortality of the treated fish exposed to bifenthrin with respect to the control at all the hours of exposure. A significant variation (p&lt;0.05) between rate of mortality of H. fossilis and time slots (24-96h) was recorded for the final selected doses of bifenthrin except 4.0, 4.5 and 5.5 µg/l concentration of the toxicant. A vigorous mucous secretion and hyper-excitability was observed in the fish at the higher doses of bifenthrin specifically at 24h and 48h time slots. There was a profound loss in equilibrium of the treated fish particularly at the higher doses at 72h and 96h time slots. The gradual increase in dose of bifenthrin resulted in significant increase (p&lt;0.05) in opercular movement of the fish with respect to the control. On the other hand, opercular movement showed a significant increase (p&lt;0.05) with the advancement of time for all the treated doses. Therefore, bifenthrin is indicated to be very strongly toxic to fish.
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48

Aktar, Mt Nur-A.-Sharmin, and Md Asek Uddin. "Effect of Probiotic Supplimentations on the Gut Histoarchitecture of Stinging Catfish, Heteropneustes fossilis." European Journal of Aquatic Sciences 4, no. 1 (2025): 1–8. https://doi.org/10.24018/ejaqua.2025.4.1.22.

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Abstract:
The study was conducted to evaluate the effect of probiotics on the gut histology of stinging catfish, Heteropneustes fossilis. The experiment was conducted in 15 ponds, each with 0.75 decimal and stocking density were 550 fingerlings/decimal (6.44 ± 0.054 gm) and cultured for 90 days (May to August). Commercially available gut probiotic ZYMETIN, water probiotic pH FIXER and soil probiotic Super PS were used in the experiment. T1 was designed with the recommended dose of Super PS (soil probiotic). T2 was supplemented with the recommended dose of gut probiotic (ZYMETIN). T3 was designed with combined application of Super PS, ZYMETIN and pH FIXER at recommended doses. T4 was designed with the recommended dose of water probiotic (pH FIXER). Only basal feed was applied for T5 (control). Water quality parameters, morphometric measurements, body weight of the experimental fish were measured, and fish gut samples were collected for histological study at fortnightly intervals. The results showed that all water quality parameters in pH FIXER treated ponds were more favorable for fish culture compared with others. In combined probiotic treated groups (T3) histoarchitecture of the gut were almost normal. Less pathological signs were observed in probiotic treated groups (T1, T2, and T4) compared with T5 (control). From analysis of gut revealed that fold length, fold width and epithelial layer thickness were increased significantly (P &lt; 0.05) in combined probiotics treated fish. Fish from T2 and T3 exhibited larger fold length, width and epithelial layer thickness than those of T1, T4, T5 (control). Probiotic addition can improve intestinal structure of H. fossilis which may increase the nutrients absorption in fish. Hence, digestion capacity was increased that ultimately helps to improve the overall health condition of stinging catfish, H. fossilis.
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49

Aktar, Mt Nur-A.-Sharmin, and Md Asek Uddin. "Effect of Probiotic Supplimentations on the Gut Histoarchitecture of Stinging Catfish, Heteropneustes fossilis." European Journal of Aquatic Sciences 4, no. 1 (2025): 1–8. https://doi.org/10.24018/5x49xk82.

Full text
Abstract:
The study was conducted to evaluate the effect of probiotics on the gut histology of stinging catfish, Heteropneustes fossilis. The experiment was conducted in 15 ponds, each with 0.75 decimal and stocking density were 550 fingerlings/decimal (6.44 ± 0.054 gm) and cultured for 90 days (May to August). Commercially available gut probiotic ZYMETIN, water probiotic pH FIXER and soil probiotic Super PS were used in the experiment. T1 was designed with the recommended dose of Super PS (soil probiotic). T2 was supplemented with the recommended dose of gut probiotic (ZYMETIN). T3 was designed with combined application of Super PS, ZYMETIN and pH FIXER at recommended doses. T4 was designed with the recommended dose of water probiotic (pH FIXER). Only basal feed was applied for T5 (control). Water quality parameters, morphometric measurements, body weight of the experimental fish were measured, and fish gut samples were collected for histological study at fortnightly intervals. The results showed that all water quality parameters in pH FIXER treated ponds were more favorable for fish culture compared with others. In combined probiotic treated groups (T3) histoarchitecture of the gut were almost normal. Less pathological signs were observed in probiotic treated groups (T1, T2, and T4) compared with T5 (control). From analysis of gut revealed that fold length, fold width and epithelial layer thickness were increased significantly (P &lt; 0.05) in combined probiotics treated fish. Fish from T2 and T3 exhibited larger fold length, width and epithelial layer thickness than those of T1, T4, T5 (control). Probiotic addition can improve intestinal structure of H. fossilis which may increase the nutrients absorption in fish. Hence, digestion capacity was increased that ultimately helps to improve the overall health condition of stinging catfish, H. fossilis.
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50

Chandra, Sudhish. "Toxic effects of endosulfan on cholesterol levels of liver, brain and gills of stinging catfish, Heteropneustes fossilis." Journal of Applied and Natural Science 3, no. 1 (2011): 93–96. http://dx.doi.org/10.31018/jans.v3i1.162.

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Abstract:
Stinging catfish Heteropneustes fossilis exposed to different concentrations of an organochlorine pesticide endosulfan under static conditions, revealed statistically significant increase in cholesterol contents of liver, brain and gill tissues even at the lowest concentration (0.0010 mg/l). Fish also elicited behavioral changes due to pesticide toxicity. Hypercholesterolemia appeared to be due to stress induced metabolic alterations caused by intoxication.
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