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Journal articles on the topic 'Shoot regeneration'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Tezuka, Takahiro, Masashi Harada, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka, and Masayuki Oda. "Effects of Auxin and Cytokinin on In Vivo Adventitious Shoot Regeneration from Decapitated Tomato Plants." HortScience 46, no. 12 (2011): 1661–65. http://dx.doi.org/10.21273/hortsci.46.12.1661.

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Adventitious shoots can be regenerated from the cut surface of the primary shoot and lateral branches in decapitated plants in vivo. This inherent regenerative ability of plants is useful for mass propagation. In the present study, we conducted histological observations of shoot regeneration and applied auxin and cytokinin to decapitated seedlings in four tomato cultivars. The cultivars produced different numbers of adventitious shoots after decapitation; ‘Petit’ produced the largest number of adventitious shoots (78.5 ± 10.2) and ‘Momotaro’ produced the fewest (12.1 ± 3.3). Histological obser

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2

Cao, X., and F. Hammerschlag. "307 Growth Regulator Pretreatments Significantly Enhance the Efficiency of Shoot Organogenesis from Leaf Explants of Highbush Blueberry Cultivar Bluecrop." HortScience 35, no. 3 (2000): 445A—445. http://dx.doi.org/10.21273/hortsci.35.3.445a.

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As part of a program to develop transgenic highbush blueberry (Vaccinium corymbosum L.) cultivars, studies were conducted to determine optimum conditions for high-efficiency shoot regeneration from leaf explants of in vitro propagated, commercially important, tissue culture-recalcitrant `Bluecrop' shoot cultures. The effects of pretreatments, growth regulators, and age of explant source on shoot organogenesis were investigated. A maximum of 98% shoot regeneration and 10 shoots regenerating per leaf explant occurred when explants of 2-week-old shoot cultures were incubated in the dark (for a to

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3

Chae, Soo Cheon, Haeng Hoon Kim, and Sang Un Park. "Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa)." Scientific World Journal 2012 (2012): 1–4. http://dx.doi.org/10.1100/2012/859381.

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Shoot organogenesis and plant regeneration inSinningia speciosawere improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl2), and silver thiosulphate (STS). The addition of AVG, CoCl2, and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performi

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4

Xu, L., G. F. Liu, and M. Z. Bao. "Adventitious Shoot Regeneration from In Vitro Leaves of Formosan Sweetgum (Liquidambar formosana L.)." HortScience 42, no. 3 (2007): 721–23. http://dx.doi.org/10.21273/hortsci.42.3.721.

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Plantlets were regenerated from in vitro-grown leaf explants of five genotypes of Liquidambar formosana on WPM basal medium supplemented with different concentrations of TDZ and NAA. With the addition of 0.27 μm NAA, regeneration efficiency was increased by 2- to 4-fold over that with TDZ alone. Lower concentrations of TDZ (0.45–2.27 μm) were beneficial for regenerating shoot clusters. Four genotypes (P2, P6, P9, and P11) showed high regeneration rates (up to 90%), whereas genotype P13 showed a low capability for shoot regeneration on all media tested (<35%). For all five genotypes, the opt

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5

Brand, Mark H. "099 Indirect and Direct Regeneration of Kalmia latifolia." HortScience 34, no. 3 (1999): 458D—458. http://dx.doi.org/10.21273/hortsci.34.3.458d.

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To introduce desirable trait genes into Kalmia latifolia, efficient adventitious shoot regeneration methods are needed. Silver Dollar (S$) callus induction and growth in the dark was compared on Woody Plant (WP) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) (1, 5, 10, 20 μM) or naphthaleneacetic acid (NAA) (1, 10, 20, 40 μM) with and without 5 μM isopentenyladenine (2iP). Both 2,4-D and NAA produced >450 mg of callus from leaf explants in 8 weeks. The addition of 2iP tripled growth for 2,4-D and doubled growth for NAA. Greatest callus growth was obtained on 20-40 μM NAA or 5-20 μ

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6

George, Mary W., and Robert R. Tripepi. "659 (PS 4) CYTOKININS AND DONOR PLANTS AFFECT REGENERATIVE CAPACITY OF AMERICAN ELM LEAVES." HortScience 28, no. 5 (1993): 545g—545. http://dx.doi.org/10.21273/hortsci.28.5.545g.

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Adventitious shoots can be regenerated from leaf explants of American elm (Ulmus americana L.), but the effects of cytokinins and donor plants were unknown. The goal of this study was to examine factors that influence regenerative capacity of American elm leaves. Excised leaves from 2-year-old seedlings were surface sterilized, and 1 cm2 sections were taken from the midrib portion of the leaves. Three to six seedlings were used as donor plants in various experiments. Zero, 7.5, 15, or 22.5 μM of benzyladenine (BA), thidiazuron (TDZ), kinetin, zeatin, or 2iP were added to Driver Kuniyuki Walnut

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7

Orlikowska, Teresa, Agnieszka Marasek, and Danuta Kucharska. "Regeneration of Paeonia mlokosewitschii Lom. and P. tenuifolia L. in vitro from different explants." Acta Societatis Botanicorum Poloniae 67, no. 3-4 (2014): 223–27. http://dx.doi.org/10.5586/asbp.1998.026.

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The pattern of regeneration from tissues of <em>Paeonia mlokosewitschii</em> and <em>P. tenuifolia</em> cultured in vitro in the same chemical conditions depended on the initial explant. Direct shoot regeneration was obtained from the bases of petioles and petals, and leaf veins. Vegetative initial buds and regenerated in vitro shoots produced on their bases slowly growing nodular callus which was very productive in repetitive shoot regeneration. The tops of stems, flower bases, sepals, petals and ovary walls produced small callus which regenerated white and red spheric

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8

Amrina, Shafi, Zahoor Insha, and A. Mir Mudasir. "Invitro Regeneration of Arabidopsis Thaliana from Suspension and Invitro Cultures and Analysis of Regeneration and Antioxidant Enzyme Levels." International Journal of Trend in Scientific Research and Development 2, no. 1 (2017): 37–45. https://doi.org/10.31142/ijtsrd5843.

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Arabidopsis tissue culture is valuable for any laboratory working on this model plant. Tissue culture methodology facilitates the production of a large number of plants that are genetically identical over a relatively short growth period. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Plant regeneration is a key technology for successful stable plant transformation, while cell suspension cultures can be exploited for metabolite profiling, kinetic study and mining. In this paper we report methods for the successf

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9

George, M. W., and R. R. Tripepi. "084 Plant Preservative Mixture (PPM) can Reduce Shoot Regeneration from Leaf Explants of Selected Plants." HortScience 34, no. 3 (1999): 455E—455. http://dx.doi.org/10.21273/hortsci.34.3.455e.

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Plant preservative mixture (PPM) is a new broad-spectrum biocide that may be useful for plant tissue culture. The objective of this study was to determine if PPM interfered with adventitious shoot regeneration on leaf explants from several plant species. Leaf explants from Dendranthema grandiflora `Iridon', Betula pendula, Rhododendron catawbiense var. album and R.c. `America' were made from the top two apical leaves on the microshoots. In the first experiment, 0, 0.5, 1, 2, or 4 mL·L-1 PPM were added to species-appropriate regeneration media. In the second experiment, only mum leaf explants w

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10

Nehra, Narender S., Cecil Stushnoff, and K. K. Kartha. "Direct Shoot Regeneration from Strawberry Leaf Disks." Journal of the American Society for Horticultural Science 114, no. 6 (1989): 1014–18. http://dx.doi.org/10.21273/jashs.114.6.1014.

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Abstract An efficient and reproducible method of direct shoot regeneration from leaf disks in strawberry (Fragaria × ananassa cv. Redcoat) has been developed. The influence of hormone concentration, light intensity, orientation of leaf disk, and age of explant source on shoot regeneration was examined. Regeneration of leaf disks reached 94%, with an average of 13 shoots per leaf disk, within 8 weeks when MS salts and B-5 vitamins medium supplemented with 10 μm each of BA and IAA were used. The adventitious shoot meristems initially arose from epidermal or sub-epidermal cells at the periphery o

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11

Cao, Xiaoling, Freddi A. Hammerschlag, and Larry Douglass. "A Two-step Pretreatment Significantly Enhances Shoot Organogenesis from Leaf Explants of Highbush Blueberry cv. Bluecrop." HortScience 37, no. 5 (2002): 819–21. http://dx.doi.org/10.21273/hortsci.37.5.819.

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As part of a program to improve highbush blueberry (Vaccinium corymbosum L.) cultivars via tissue culture and genetic engineering, studies were conducted to determine optimum conditions for organogenesis from leaf explants of the previously recalcitrant cv. Bluecrop. The effects of a pretreatment, growth regulators, and age of explant source on shoot organogenesis were investigated. A maximum of 98% explants regenerated shoots with a mean of 11 shoots per leaf explant after 62 days when explants of 2-week-old shoot cultures were incubated on the following regime: pretreatment medium #1 contain

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12

Hebert, Cary J., Darren H. Touchell, Thomas G. Ranney, and Anthony V. LeBude. "In Vitro Shoot Regeneration and Polyploid Induction of Rhododendron ‘Fragrantissimum Improved’." HortScience 45, no. 5 (2010): 801–4. http://dx.doi.org/10.21273/hortsci.45.5.801.

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Rhododendron L.‘Fragrantissimum Improved’ is an attractive cultivar with showy, fragrant flowers but has limited potential for breeding because it is a sterile wide hybrid. Protocols for in vitro regeneration and polyploid induction were developed for this cultivar as a means to potentially restore fertility and enhance ornamental traits. Combinations of thidiazuron (TDZ) at 0, 5, 10, 15, or 20 μM and 1-naphthaleneacetic acid (NAA) at 0, 2.5, 5, or 10 μM were used to induce shoot regeneration from leaves. Shoot regeneration was optimized (68% of leaf segments produced shoots) using 8.8 μM TDZ

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13

Qu, Luping, James Polashock, and Nicholi Vorsa. "527 A Highly Efficient in Vitro Cranberry Regeneration System using Leaf Explants." HortScience 34, no. 3 (1999): 536D—536. http://dx.doi.org/10.21273/hortsci.34.3.536d.

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We have established a very efficient cranberry regeneration (shoot organogenesis) system from leaf explants using a basal medium consisting of Anderson's salts and Murashige and Skoog (MS) organics supplemented with 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (TDZ) and N6-(-??-dimethyallylamino) purine) (2ip). Characteristics examined include combinations of varying levels of three plant growth regulators (TDZ, 2ip, and naphthaleneacetic acid (NAA), explant orientation (adaxial or abaxial side in contact with the media), and leaf position relative to the distal end of the shoot. Genotypes (`Early

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14

Phan, Thi Thu Hien. "Research on the one-step regeneration of sugarcane in KK3 variety (Saccharum officinarum L.) in vitro." Ministry of Science and Technology, Vietnam 64, no. 5 (2022): 70–74. http://dx.doi.org/10.31276/vjst.64(5).70-74.

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The research has successfully created the formula for direct regeneration medium of sugarcane variety KK3. In detail, the most suitable medium for direct regeneration from young leaf rolls of sugarcane variety KK3 was RE2 (MS + 5 mg/l α-NAA + 1 mg/l Kinetin), the regeneration rate was 25.27%, the number of shoots formed/1.0 g of young leaf rolls reached 11.56 shoots. The study showed that the position of the young leaf rolls cuttings suitable for direct regeneration was the cuttings located from 2-6 cm away from the growth apex. The shoot regeneration rate reached 25.40%, the regenerating samp

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15

Zhang, Yan, and Prem L. Bhalla. "In vitro shoot regeneration from commercial cultivars of Australian canola (Brassica napus L.)." Australian Journal of Agricultural Research 55, no. 7 (2004): 753. http://dx.doi.org/10.1071/ar03209.

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Canola, (Brassica napus L.) is an important crop in Australia. Large genetic variability in the Australian canola cultivars is reflected by their diverse agronomic characteristics. Further improvement using modern breeding methods will lead to the generation of better canola varieties suited for Australian conditions. Genetic engineering relies on the development of efficient methods for regeneration of viable shoots from cultured tissues, and the successful application of transformation techniques. This study reports the in vitro shoot regeneration potential from seedling explants of 7 commer

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16

Kapaun, James A., and Zong-Ming Cheng. "Plant Regeneration from Leaf Tissues of Siberian Elm." HortScience 32, no. 2 (1997): 301–3. http://dx.doi.org/10.21273/hortsci.32.2.301.

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Plants were regenerated from leaf tissue of greenhouse-grown seedlings of Siberian elm (Ulmus pumila L.). Shoot regeneration was induced on Murashige and Skoog (MS) medium containing 5 to 10 μm of BA. Up to 55% of the leaf explants formed shoots with an average of 2.4 shoots per explant. Addition of 2.5 or 5 μm of IBA failed to enhance regeneration. Thidiazuron at 0.5 or 1.0 μm also induced shoot regeneration, but the shoots failed to elongate as well as shoots regenerated from media containing BA. Incubation in darkness for 7, 14, or 21 d had little effect in promoting shoot regeneration, exc

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17

Stamp, James A., Sheila M. Colby, and Carole P. Meredith. "Improved Shoot Organogenesis from Leaves of Grape." Journal of the American Society for Horticultural Science 115, no. 6 (1990): 1038–42. http://dx.doi.org/10.21273/jashs.115.6.1038.

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Adventitious shoots developed within 3 weeks from the petiolar stub and, less often, from wounded lamina tissues when leaves excised from nodal cultures of Vitis vinifera L. cvs. French Colombard and Thompson Seedless were cultured on solid Nitsch and Nitsch medium containing BAP at 2 mg·liter-1. The youngest leaf that could be excised, from 1 to 8 mm long, was the most responsive (90% of explants producing shoots compared to 16% for leaf 6). Removal of the lamina from the petiolar stub within the first 3 weeks of culture reduced shoot production. Increase in nodal culture age, without transfe

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18

Billings, Sharon G., Chee K. Chin, and Gojko Jelenkovic. "Regeneration of Blueberry Plantlets from Leaf Segments." HortScience 23, no. 4 (1988): 763–66. http://dx.doi.org/10.21273/hortsci.23.4.763.

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Abstract A procedure to regenerate shoots in vitro from leaf sections of two blueberry genotypes, ‘Berkeley’ and ‘Bluehaven’, in Woody Plant Medium was established. The effect of 20 combinations of growth regulators: NAA at 0, 1, 2, and 4 µM and 2iP at 0, 5, 10, 15, and 20 µM were compared. Combinations containing NAA generally promoted callus formation and suppressed shoot regeneration. Conversely, media containing cytokinin promoted shoot regeneration and suppressed callus formations. The optimal medium for shoot regeneration contained 15 µM 2iP but no NAA. This procedure was highly efficien

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19

Miranda, Jacintha, Michele N. Konschuh, E. C. Yeung, and C. C. Chinnappa. "In vitro plantlet regeneration from hypocotyl explants of Stellaria longipes (Caryophyllaceae)." Canadian Journal of Botany 77, no. 2 (1999): 318–22. http://dx.doi.org/10.1139/b99-024.

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An in vitro regeneration protocol for Stellaria longipes Goldie was developed using young hypocotyl explants. Optimal regeneration was obtained using Murashige and Skoog (MS) basal medium supplemented with 0.5 µM N6-benzyladenine and 1 µM indole-3-butyric acid. Three different patterns of shoot regeneration were observed: (i) "direct shoot" formation within 3-5 days of inoculation, (ii) nodular structures appeared followed by shoot formation, and (iii) callus formation followed by the appearance of shoots. Histological observation revealed that cells within the central vascular cylinder of the

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20

Jin, Wanmei, Yuanhua Wang, and Hua Wang. "Adventitious shoot regeneration from leaves of apple rootstock ‘Pingyitiancha’ (Malus hupehensis var. pinyiensis) and genetic fidelity of regenerated plantlets using SSR markers." Canadian Journal of Plant Science 94, no. 8 (2014): 1345–54. http://dx.doi.org/10.4141/cjps2013-357.

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Jin, W., Wang, Y. and Wang, H. 2014. Adventitious shoot regeneration from leaves of apple rootstock ‘Pingyitiancha’ (Malus hupehensis var. pinyiensis) and genetic fidelity of regenerated plantlets using SSR markers. Can. J. Plant Sci. 94: 1345–1354. Apple is one of the major fruit tree species in China, its cultivation area and total output rank first in the world. ‘Pingyitiancha’ (Malus hupehensis var. pinyiensis) is a widely used rootstock for apple cultivation in China. Several factors affecting leaf regeneration were investigated. In this study, a successful adventitious shoot regeneration

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21

Chen, Yuan, Yumei Xie, Keyuan Zheng, Yanru Fan, Huijing Zhou, and Mulan Zhu. "Regeneration Capability Comparison of Leaves Between Nodal Cuttings from Young Stems and Suckers and Its Histological Analysis in Triadica sebifera." Forests 16, no. 6 (2025): 992. https://doi.org/10.3390/f16060992.

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Triadica sebifera, an economically and medicinally valuable tree species native to China, was investigated for its in vitro regeneration potential using leaf explants from nodal cuttings of young stems and sprouts. This study evaluated the effects of basal media, plant growth regulators (PGRs), explant sources, and incision methods on adventitious shoot induction, supplemented by histological analysis. The highest shoot regeneration frequency (98.89%) and maximum shoot number (72) were achieved via direct organogenesis using sucker-derived nodal cuttings cultured on MS medium with 2 mg/L 6- be

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22

Sorvari, S., S. Ulvinen, T. Hietaranta, and H. Hiirsalmi. "Preculture Medium Promotes Direct Shoot Regeneration from Micropropagated Strawberry Leaf Disks." HortScience 28, no. 1 (1993): 55–57. http://dx.doi.org/10.21273/hortsci.28.1.55.

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The effect of preculturing in vitro plantlets of two strawberry (Fragaria ×ananassa Duch.) cultivars grown on micropropagation medium with and without hormones on regenerating shoots from leaf disks was examined. Preculturing stock plants on micropropagation medium with hormones (BAP at 0.5 mg·liter-1 + IBA at 0.5 mg·liter-1 GA, at 0.2 mg·liter-1) promoted shoot regeneration in the two cultivars tested. Using hormone-containing micropropagation medium for preculture, the highest mean regeneration rate of 9.9 shoots per total number of leaf disks was obtained for the Finnish cultivar Hiku on mo

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23

Subban, Patharajan, Yaarit Kutsher, Dalia Evenor, et al. "Shoot Regeneration Is Not a Single Cell Event." Plants 10, no. 1 (2020): 58. http://dx.doi.org/10.3390/plants10010058.

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Shoot regeneration is a key tool of modern plant biotechnology. While many researchers use this process empirically, very little is known about the early molecular genetic factors and signaling events that lead to shoot regeneration. Using tobacco as a model system, we found that the inductive events required for shoot regeneration occur in the first 4–5 days following incubation on regeneration medium. Leaf segments placed on regeneration medium did not produce shoots if removed from the medium before four days indicating this time frame is crucial for the induction of shoot regeneration. Lea

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24

Cao, X., and F. A. Hammerschlag. "Improved Shoot Organogenesis from Leaf Explants of Highbush Blueberry." HortScience 35, no. 5 (2000): 945–47. http://dx.doi.org/10.21273/hortsci.35.5.945.

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As part of a program to develop transgenic highbush blueberry (Vaccinium corymbosum L.) cultivars, studies were conducted to determine optimum conditions for high efficiency shoot regeneration from leaf explants of shoots propagated in vitro. The effects on shoot organogenesis of age of explant source, length of dark treatment, the addition of either thidiazuron (TDZ) at 1 or 5 μm, or zeatin riboside at 20 μm to the regeneration medium, and a photosynthetic photon flux (PPF) of either 18 ± 5 or 55 ± 5 μmol·m–2·s–1 were investigated. A maximum of 13.0, 13.0, 12.6, and 4.6 shoots regenerating pe

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25

Al-Khayri, Jameel M., Feng H. Huang, Teddy E. Morelock, and Tahani A. Busharar. "Spinach Tissue Culture Improved with Coconut Water." HortScience 27, no. 4 (1992): 357–58. http://dx.doi.org/10.21273/hortsci.27.4.357.

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A preliminary study has shown that the addition of 15% (v/v) coconut water (CW) to the culture medium significantly improved callus growth, shoot-regenerative capacity, and shoot growth in leaf disk cultures of spinach (Spinacia oleracea L.). Subsequently, the influence of a range of CW concentrations, 0%, 5%, 10%, 15%, or 20% (v/v), was examined. Callus weight obtained after 5 weeks showed direct relationship to the concentration of CW. This stimulator action was observed in both cultivars tested in this study, `High Pack' and `Baker'. On CW-containing medium, shoot regeneration was expedited

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26

Neves, Mariana, Sandra Correia, and Jorge Canhoto. "Ethylene Inhibition Reduces De Novo Shoot Organogenesis and Subsequent Plant Development from Leaf Explants of Solanum betaceum Cav." Plants 12, no. 9 (2023): 1854. http://dx.doi.org/10.3390/plants12091854.

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In de novo shoot organogenesis (DNSO) plant cells develop into new shoots, without the need of an existing meristem. Generally, this process is triggered by wounding and specific growth regulators, such as auxins and cytokinins. Despite the potential significance of the plant hormone ethylene in DNSO, its effect in regeneration processes of woody species has not been thoroughly investigated. To address this gap, Solanum betaceum Cav. was used as an experimental model to explore the role of this hormone on DNSO and potentially extend the findings to other woody species. In this work it was show

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27

Le, Thanh Tung, and Thi Mat Pham. "Study on the effect of explant size on direct shoot regeneration capability of seaweed Kappaphycus alvarezii(Rhodophyta, Solieriaceae) in Vietnam." Ministry of Science and Technology, Vietnam 66, no. 5 (2024): 75–80. http://dx.doi.org/10.31276/vjst.66(5).75-80.

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Direct regeneration is a biological technique used to create a large quantity of seaweed seedlings from mother plants. In this experiment, Kappaphycus alvarezii, ranging in size 0.25-2.0 cm, was cultivated in a Provasoli's Enriched Seawater (PES) medium supplemented with 5 mg/l of Indole-3-acetic acid combined with 1 mg/l of N6-benzyladenine. After 60 days of the experiment, the size of the explant significantly affected the direct regenerative capability of Kappaphycus alvarezii. The 0.25 cm-sized explants showed the lowest survival and regeneration rates 13.33±3.34%, with an average shoot co

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WANG, X. H., Q. M. HUANG, L. WANG, and L. Z. WANG. "EFFECT OF SINGLE-WALL CARBON NANOTUBE ON SOYBEAN (GLYCINE MAX) REGENERATION FROM MATURE COTYLEDONARY NODE EXPLANTS." Nano LIFE 02, no. 04 (2012): 1250014. http://dx.doi.org/10.1142/s1793984412500146.

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One of the major limitations in producing transgenic soybeans using the agrobacterium-mediated cotyledonary-node method is low regeneration frequency. An improved highly efficient regeneration system of soybean was established herein. Cotyledonary node explants were placed in shoot initiation medium with single-wall carbon nanotube (SWNT) for adventitious shoots regeneration, and adventitious shoots were subcultured in shoot elongation medium with SWNT for shoot elongation and rooting. 40 mg/L SWNT supplemented in shoot initiation medium was found to be the optimal concentration with shoots re

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29

Debnath, Samir C. "A Two-step Procedure for Adventitious Shoot Regeneration from in vitro-derived Lingonberry Leaves: Shoot Induction with TDZ and Shoot Elongation Using Zeatin." HortScience 40, no. 1 (2005): 189–92. http://dx.doi.org/10.21273/hortsci.40.1.189.

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The effects of TDZ (0, 0.1, 1, 5 and 10 μm) and explant orientation on adventitious shoot regeneration of `Erntedank' lingonberry were studied. Moderate concentration (1 to 5 μm) of TDZ supported bud and shoot regeneration, but strongly inhibited shoot elongation. TDZ initiated cultures were transferred to medium containing 1-2 μm zeatin and produced usable shoots after one additional subculture. Adventitious bud and shoot regeneration was greatly influenced by explant orientation. Elongated shoots were rooted on a 2 peat: 1 perlite (v/v) medium, and the plantlets were acclimatized and eventua

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Cao, Xiaoling, and F. A. Hammerschlag. "112 The Influence of Plant Growth Regulators and Light Levels on Shoot Morphogenesis from Leaf Explants of Highbush Blueberry." HortScience 34, no. 3 (1999): 460F—461. http://dx.doi.org/10.21273/hortsci.34.3.460f.

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As part of a program to develop transgenic highbush blueberry (Vaccinium corymbosum L.) cultivars, studies were conducted to determine optimum conditions for high efficiency shoot regeneration from leaf explants of in vitro-propagated shoot cultures. The effect of either thidiazuron at 1 or 5 μM, or zeatin riboside at 20 μM, and two lit levels (18 ± 5 or 55 ± 5 μmol·m-2·s-1) on shoot organogenesis were investigated. With the exception of `Bluecrop', which did not regenerate shoots, maximum shoot regeneration of 13, 12.7, 12.6 and 4.6 shoots per explant for cultivars Duke, Georgiagem, Sierra, a

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31

Raspor, Martin, Václav Motyka, Abdul Rasheed Kaleri, et al. "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs." International Journal of Molecular Sciences 22, no. 16 (2021): 8554. http://dx.doi.org/10.3390/ijms22168554.

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De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this process, and genes involved in their signaling cascades act as master regulators of the different phases of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration are often inter

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Faize, Mohamed, Lydia Faize, Lorenzo Burgos, Alan T. Critchley, and Nuria Albuquerque. "Application of Ascophyllum nodosum-Based Soluble Extract on Micropropagation and Regeneration of Nicotiana benthamiana and Prunus domestica." Plants 10, no. 7 (2021): 1354. http://dx.doi.org/10.3390/plants10071354.

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In the present study, the effect of a commercial extract of the seaweed Ascophyllum nodosum on in vitro micropropagation, shoot regeneration, and rhizoghenesis were studied in Nicotiana benthamiana and Prunus domestica. Results showed that the MS medium supplemented with various concentrations of the Ascophyllum extract (5, 10, 50, and 100 mg L−1) significantly enhanced the number of regenerated buds from N. benthamiana leaf discs to the conventional MS regenerating medium. Increases ranged from 3.5 to 6.5 times higher than the control. The effect of the Ascophyllum extract on N. benthamiana m

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Fu, Dong, Tan, et al. "Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv." Genes 10, no. 12 (2019): 1034. http://dx.doi.org/10.3390/genes10121034.

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De novo shoot regeneration is one of the important manifestations of cell totipotency in organogenesis, which reflects a survival strategy organism evolved when facing natural selection. Compared with tissue regeneration, and somatic embryogenesis, de novo shoot regeneration denotes a shoot regeneration process directly from detatched or injured tissues of plant. Studies on plant shoot regeneration had identified key genes mediating shoot regeneration. However, knowledge was derived from Arabidopsis; the regeneration capacity is hugely distinct among species. To achieve a comprehensive underst

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Smith, Nicole A., and Prem L. Bhalla. "Comparison of shoot regeneration potential from seedling explants of Australian cauliflower (Brassica oleracea var. botrytis) varieties." Australian Journal of Agricultural Research 49, no. 8 (1998): 1261. http://dx.doi.org/10.1071/a98067.

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Genetic engineering of crop plants relies on the development of efficient methods for the regeneration of viable shoots from cultured tissues. The objective of the present study was to develop a protocol for efficient shoot and plant regeneration from seedling explants of commercial cauliflower (B. oleracea var. botrytis) genotypes and to compare the regeneration capacity of the most commonly used explants: cotyledon, hypocotyl, and root. A combination of growth hormones including 6-benzylaminopurine, 1-naphthylacetic acid, and gibberellic acid was used in the MS-based medium, and factors infl

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Swanberg, Andrea, and Wenhao Dai. "Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis." HortScience 43, no. 3 (2008): 832–36. http://dx.doi.org/10.21273/hortsci.43.3.832.

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Two periwinkle cultivars, Pacific Coral (P1) and Sunstorm Rose (P2), were used for development of a plant regeneration system. Leaf and internodal explants collected from in vitro plants were plated onto woody plant medium (WPM) using a factorial arrangement of 6-benzyladeine (BA) and 1-naphthalene acetic acid (NAA). Shoots were successfully regenerated. Shoot production from leaf tissues was minimal for all cultivars, whereas internodal tissues showed variable rates of regeneration depending on the hormone combination. Cultivar P1 showed the maximum regeneration rate (73.3%) when internodal e

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Debnath, Samir C., and Danny L. Barney. "Shoot Regeneration and Plantlet Formation by Cascade Huckleberry, Mountain Huckleberry, and Oval-leaf Bilberry on a Zeatin-containing Nutrient Medium." HortTechnology 22, no. 1 (2012): 106–13. http://dx.doi.org/10.21273/horttech.22.1.106.

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A plant regeneration protocol was developed for cascade huckleberry (Vaccinium deliciosum), mountain huckleberry (V. membranaceum), and oval-leaf bilberry (V. ovalifolium) clones. The effects of zeatin concentrations (0, 4.6, 9.1, and 13.7 μM) and explant type (leaf or stem segment) on adventitious shoot regeneration were studied on a nutrient medium of low ionic concentration. Adventitious bud and shoot regeneration was greatly influenced by clone, explant type, and zeatin concentration. Zeatin at 9.1 to 13.7 μM supported the best bud and shoot regeneration. At low concentrations (2.3 to 4.6

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Assia, Zahoor, Qadir Jasfeeda, and Seema Singh Dr. "Effect of Different Carbon Sources on in Vitro Shoot Regeneration of Petunia Hybrid Hort. Vilm." International Journal of Trend in Scientific Research and Development 2, no. 4 (2018): 2540–42. https://doi.org/10.31142/ijtsrd15641.

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The present study was undertaken in order to develop an efficient in vitro propagation protocol for Petunia hybrid using leaf as an explant. The effects of different concentrations of five different carbon sources i.e., sucrose, glucose, mannose, arabinose and maltose was investigated on shoot regeneration of Petunia hybrid. Shoot regeneration occurred on MS medium supplemented with sucrose, glucose and maltose. No growth was obtained on MS medium when mannose and arabinose were used as carbon source. Best shoot regeneration with maximum shoot length and maximum number of shoots was obtained o

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Narasimhulu, S. B., Shyam Prakash, V. L. Chopra, and V. Arunachalam. "Comparative shoot regeneration in diploid and amphidiploid Brassica species and their interspecific hybrids." Canadian Journal of Botany 70, no. 7 (1992): 1513–14. http://dx.doi.org/10.1139/b92-189.

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Shoot regeneration response in interspecific hybrids of Brassica species were assessed in relation to the diploid pollen parents and amphidiploid female parents. Superior regeneration responses were observed in interspecific combinations of B. carinata and B. nigra (BBC), B. juncea and B. campestris (AAB), and B. napus and B. campestris (AAC). Though synthetic B. napus regenerated with a frequency less than that of the better regenerating parent (B. oleracea), higher regeneration response was observed in the hybrid between B. napus and B. campestris. Two triploid combinations of the genetic co

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Barik, Durga Prasad, Umaballava Mohapatra, and Pradeep Kumar Chand. "Direct shoot regeneration from epicotyl explants of grasspea (Lathyrus sativus)." Australian Journal of Botany 54, no. 5 (2006): 505. http://dx.doi.org/10.1071/bt05152.

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A reproducible procedure is described for adventitious shoot organogenesis in epicotyl segments resulting in prolific plant regeneration of a grain legume grasspea (Lathyrus sativus L.). Among seedling explant types examined, epicotyl segments were most responsive. The highest percentage of direct shoot regeneration was elicited on Murashige–Skoog (MS) medium augmented with 4.0 mg L–1 6-benzyladenine (BA) + 2.0 mg L–1 α-naphthaleneacetic acid (NAA). Compared with four other genotypes examined, IC-120487 showed the highest shoot regeneration frequency (approximately 80%) with maximum shoot numb

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Kochanová, Zuzana, Naci Onus, and Ján Brindza. "Adventitious shoot regeneration from dormant buds of persimmon (Diospyros kaki Thunb.) cv. Hachiya." Journal of Agrobiology 28, no. 2 (2011): 113–18. http://dx.doi.org/10.2478/v10146-011-0012-9.

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Adventitious shoot regeneration from dormant buds of persimmon (Diospyros kakiThunb.) cv. HachiyaThe effects of plant hormones 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) on adventitious shoot regeneration from dormant persimmon buds were studied. The object of the study was the persimmon cultivar Hachiya, one of the most important persimmon cultivars in the Mediterranean area and Asia. Shoot regeneration was evaluated 4, 6 and 8 weeks after initiating the hormone treatment. Average shoot length was measured after 8 weeks and was evaluated by LSD test. Except for the media withou

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Mitić, Nevena, Mariana Stanišić, Jelena Milojević, et al. "Optimization of In Vitro Regeneration from Leaf Explants of Apple Cultivars Golden Delicious and Melrose." HortScience 47, no. 8 (2012): 1117–22. http://dx.doi.org/10.21273/hortsci.47.8.1117.

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An efficient in vitro shoot regeneration method from leaf explants of apple cultivars Golden Delicious and Melrose by optimization of regeneration medium, explant type and orientation, dark pre-treatment, and gelling agent is presented. Murashige and Skoog’s regeneration medium containing 22 μM thidiazuron (TDZ) and 1.5 μM indole-3-butyric acid (IBA) (M2 medium) was superior for regeneration as well as for subsequent shoot multiplication in both cultivars, providing regeneration frequency of 95% or higher in the best combination with other factors. Pre-incubation in the dark proved to be an es

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Song, Huijuan, Zhongquan Chen, Jiali Yan, et al. "Optimization of an Efficient Direct Shoot Induction System and Histological Evaluation of Shoot Formation in Cucurbita maxima Duch." Horticulturae 10, no. 2 (2024): 165. http://dx.doi.org/10.3390/horticulturae10020165.

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Improving plant regeneration ability and shortening regeneration time can promote the development of genetic transformation breeding technology for horticultural crops. We optimized several culture conditions, including explant type, mother plant genotype, and medium, to improve shoot formation in winter squash (Cucurbita maxima Duch.). Histological analysis of the occurrence of shoots was also carried out. The results indicate that cotyledon was the most suitable explant for inducing the shoot regeneration of winter squash. We found that ‘Jin-li’ had a shorter shoot induction time and a highe

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Yang, Guochen, and Marihelen Kamp-Glass. "Direct Shoot Organogenesis of Medicago sativa." HortScience 31, no. 4 (1996): 628f—628. http://dx.doi.org/10.21273/hortsci.31.4.628f.

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An efficient and reliable protocol of in vitro shoot regeneration must be first established to have a successful genetic transformation. As a member of legume family, alfalfa is very difficult for direct shoot regeneration. There is no published information on direct shoot organogenesis, although success has been well documented on embryogenesis, which must go through callus stage. Different plant growth regulators at various concentrations were evaluated for callus initiation, development, and direct shoot regeneration. Multiple shoots were produced directly from each individual explant. This

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Malek, MA, MA Bari Miah, M. AL-Amin, D. Khanam, and M. Khatun. "In vitro regeneration in pointed gourd." Bangladesh Journal of Agricultural Research 32, no. 3 (2008): 461–71. http://dx.doi.org/10.3329/bjar.v32i3.548.

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An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in pointed gourd. Highest percent of shoot regeneration was 93.86 when nodal explants were cultured on MS+2.0 mg/1 BAP. The maximum number of shoots (4.00) per explant was observed in MS + 2.0 mg/1 BAP + 0.3 mg/1 NAA from nodal segment. Among the two explants, nodal segment was found better for shoot regeneration. Female genotypes responded better than the male genotypes for shoot induction and proliferation. Lower nodal segment performed the best shoot regeneration. The best respons

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Ricci, Angela, Luca Capriotti, Bruno Mezzetti, Oriano Navacchi, and Silvia Sabbadini. "Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536." Plants 9, no. 6 (2020): 755. http://dx.doi.org/10.3390/plants9060755.

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In the present study, an efficient system for the in vitro regeneration of adventitious shoots from the peach rootstock Hansen 536 leaves has been established. Twenty regeneration media containing McCown Woody Plant Medium (WPM) as a basal salt supplemented with different concentrations and combinations of plant growth regulators (PGRs) were tested. Expanded leaves along with their petiole from 3-week-old elongated in vitro shoot cultures were used as starting explants. The highest regeneration rate (up to 53%) was obtained on WPM basal medium enriched with 15.5 μM N6-benzylaminopurine (BAP).

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Kupriyanova, E. V., E. R. Denisova, M. A. Baier, and T. A. Ezhova. "Differences in the Manifestation of Cell Pluripotence In Vivo and In Vitro in the Mutant Arabidopsis thaliana with the Phenotype of Cell Memory Disorder." Russian Journal of Plant Physiology 68, no. 1 (2021): 46–55. http://dx.doi.org/10.1134/s1021443721010106.

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AbstractPlant cells cultivated in vitro are a convenient model for studying the genetic and physiological mechanisms necessary for the cells to acquire a state of pluripotency. Earlier studies on a model plantArabidopsis thaliana(L.) Heynh. have identified the key role of genes that determine the pluripotency of cells in the shoot apical meristem in de novo shoot regeneration in tissue culture. In accordance with this, cells of mutant plants with a higher level of expression of pluripotency genes were characterized by an increased potential for de novo shoot regeneration. Thetaemutant was the

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Chiam, Nyet-Cheng, Tomoyo Fujimura, Ryosuke Sano, et al. "Nonsense-Mediated mRNA Decay Deficiency Affects the Auxin Response and Shoot Regeneration in Arabidopsis." Plant and Cell Physiology 60, no. 9 (2019): 2000–2014. http://dx.doi.org/10.1093/pcp/pcz154.

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Abstract Plants generally possess a strong ability to regenerate organs; for example, in tissue culture, shoots can regenerate from callus, a clump of actively proliferating, undifferentiated cells. Processing of pre-mRNA and ribosomal RNAs is important for callus formation and shoot regeneration. However, our knowledge of the roles of RNA quality control via the nonsense-mediated mRNA decay (NMD) pathway in shoot regeneration is limited. Here, we examined the shoot regeneration phenotypes of the low-beta-amylase1 (lba1)/upstream frame shift1-1 (upf1-1) and upf3-1 mutants, in which the core NM

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Yang, Woorim, Myung-Hwan Choi, Bosl Noh, and Yoo-Sun Noh. "De Novo Shoot Regeneration Controlled by HEN1 and TCP3/4 in Arabidopsis." Plant and Cell Physiology 61, no. 9 (2020): 1600–1613. http://dx.doi.org/10.1093/pcp/pcaa083.

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Abstract Plants have the ability to regenerate whole plant body parts, including shoots and roots, in vitro from callus derived from a variety of tissues. However, the underlying mechanisms for this de novo organogenesis, which is based on the totipotency of callus cells, are poorly understood. Here, we report that a microRNA (miRNA)-mediated posttranscriptional regulation plays an important role in de novo shoot regeneration. We found that mutations in HUA ENHANCER 1 (HEN1), a gene encoding a small RNA methyltransferase, cause cytokinin-related defects in de novo shoot regeneration. A hen1 mu

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Prakash, D. P., B. S. Deepali, Y. L. Ramachandra, Lalitha Anand, and Vageeshbabu S. Hanur. "Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant." Journal of Horticultural Sciences 7, no. 2 (2012): 203–5. http://dx.doi.org/10.24154/jhs.v7i2.376.

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In the present study, effect of size and age of hypocotyl explant on in vitro organogenetic responses was assessed in eggplant cv. Manjarigota. Size and age did not affect callus-initiation response, but showed marked influence on shoot regeneration response. Hypocotyl explants 1.5cm long showed highest shoot regeneration response (77.4%); either increase or decrease in size resulted in reduced response. Five to 15 day old hypocotyl explants showed direct shoot regeneration from cut ends, whereas 20-30 day old hypocotyl explants showed indirect shoot regeneration from callus produced on cut en

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Iwase, Akira, Hirofumi Harashima, Momoko Ikeuchi, et al. "WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis." Plant Cell 29, no. 1 (2016): 54–69. http://dx.doi.org/10.1105/tpc.16.00623.

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