Relevant bibliographies by topics / Single-cell membrane depolarization assay

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'Single-cell membrane depolarization assay'

Author: Grafiati
Published: 11 March 2023
Last updated: 31 July 2025

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Journal articles on the topic "Single-cell membrane depolarization assay"

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Cruz-Cruz, R., A. Salgado, C. Sánchez-Soto, L. Vaca та M. Hiriart. "Thapsigargin-sensitive cationic current leads to membrane depolarization, calcium entry, and insulin secretion in rat pancreatic β-cells". American Journal of Physiology-Endocrinology and Metabolism 289, № 3 (2005): E439—E445. http://dx.doi.org/10.1152/ajpendo.00082.2005.

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Glucose-induced insulin secretion by pancreatic β-cells depends on membrane depolarization and [Ca2+]i increase. We correlated voltage- and current-clamp recordings, [Ca2+]i measurements, and insulin reverse hemolytic plaque assay to analyze the activity of a thapsigargin-sensitive cationic channel that can be important for membrane depolarization in single rat pancreatic β-cells. We demonstrate the presence of a thapsigargin-sensitive cationic current, which is mainly carried by Na+. Moreover, in basal glucose concentration (5.6 mM), thapsigargin depolarizes the plasma membrane, producing ele
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Lunde, Christopher S., Stephanie R. Hartouni, James W. Janc, Mathai Mammen, Patrick P. Humphrey, and Bret M. Benton. "Telavancin Disrupts the Functional Integrity of the Bacterial Membrane through Targeted Interaction with the Cell Wall Precursor Lipid II." Antimicrobial Agents and Chemotherapy 53, no. 8 (2009): 3375–83. http://dx.doi.org/10.1128/aac.01710-08.

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ABSTRACT Telavancin is an investigational lipoglycopeptide antibiotic currently being developed for the treatment of serious infections caused by gram-positive bacteria. The bactericidal action of telavancin results from a mechanism that combines the inhibition of cell wall synthesis and the disruption of membrane barrier function. The purpose of the present study was to further elucidate the mechanism by which telavancin interacts with the bacterial membrane. A flow cytometry assay with the diethyloxacarbocyanine dye DiOC2(3) was used to probe the membrane potential of actively growing Staphy
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Bedut, Stephane, Christine Seminatore-Nole, Veronique Lamamy, et al. "High-throughput drug profiling with voltage- and calcium-sensitive fluorescent probes in human iPSC-derived cardiomyocytes." American Journal of Physiology-Heart and Circulatory Physiology 311, no. 1 (2016): H44—H53. http://dx.doi.org/10.1152/ajpheart.00793.2015.

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Cardiomyocytes derived from human embryonic stem cells (hESCs) or induced pluripotent stem cells (hiPSCs) are increasingly used for in vitro assays and represent an interesting opportunity to increase the data throughput for drug development. In this work, we describe a 96-well recording of synchronous electrical activities from spontaneously beating hiPSC-derived cardiomyocyte monolayers. The signal was obtained with a fast-imaging plate reader using a submillisecond-responding membrane potential recording assay, FluoVolt, based on a newly derived voltage-sensitive fluorescent dye. In our con
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Wang, Lijun, Chengbiao Zhang, Xiaotong Su, and Daohong Lin. "Kcnj10 is a major type of K+ channel in mouse corneal epithelial cells and plays a role in initiating EGFR signaling." American Journal of Physiology-Cell Physiology 307, no. 8 (2014): C710—C717. http://dx.doi.org/10.1152/ajpcell.00040.2014.

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We used primary mouse corneal epithelial cells (pMCE) to examine the role of Kcnj10 in determining membrane K+ conductance and cell membrane potential and in regulating EGF/TGFA release. Western blot, immunostaining, and RT-PCR detected the expression of Kcnj10 in mouse cornea. The single channel recording identified the 20-pS inwardly rectifying K+ channels in pMCE of WT mice, but these channels were absent in Kcnj10 −/−. Moreover, the whole cell recording demonstrates that deletion of Kcnj10 largely abolished the inward K+ currents and depolarized the cell membrane K+ reversal potential (an
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Byrd, J. C., R. Lapalombella, A. Ramanunni, et al. "Effect of CD37 small modular immuno-pharmaceutical (SMIP) on direct apoptosis in chronic lymphocytic leukemia cells via transcriptional up-regulation of the BH3 family member BIM." Journal of Clinical Oncology 27, no. 15_suppl (2009): 3035. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.3035.

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3035 Background: CD37 is a tetraspan transmembrane family protein selectively expressed on normal and transformed B-cells. A novel CD37SMIP was previously demonstrated to mediate superior direct apoptosis and NK-cell mediated killing of chronic lymphocytic leukemia (CLL) and other B-cell malignancies. Methods: Given the superior in vitro apoptosis observed with CD37SMIP treatment and early clinical activity observed in highly refractory CLL patients, we hypothesized that a unique mechanism of cell killing was utilized by CD37SMIP. This was pursued in preclinical studies outlined below. Results
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de Sousa Portilho, Adrhyann Jullyanne, Emerson Lucena da Silva, Emanuel Cintra Austregésilo Bezerra, et al. "1,4-Naphthoquinone (CNN1) Induces Apoptosis through DNA Damage and Promotes Upregulation of H2AFX in Leukemia Multidrug Resistant Cell Line." International Journal of Molecular Sciences 23, no. 15 (2022): 8105. http://dx.doi.org/10.3390/ijms23158105.

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The multidrug resistance (MDR) phenotype is one of the major obstacles in the treatment of chronic myeloid leukemia (CML) in advantage stages such as blast crisis. In this scenario, more patients develop resistance mechanisms during the course of the disease, making tyrosine kinase inhibitors (TKIs) target therapies ineffective. Therefore, the aim of the study was to examine the pharmacological role of CNN1, a para-naphthoquinone, in a leukemia multidrug resistant cell line. First, the in vitro cytotoxic activity of Imatinib Mesylate (IM) in K-562 and FEPS cell lines was evaluated. Subsequentl
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Crnich, Rachael, Gregory C. Amberg, M. Dennis Leo, et al. "Vasoconstriction resulting from dynamic membrane trafficking of TRPM4 in vascular smooth muscle cells." American Journal of Physiology-Cell Physiology 299, no. 3 (2010): C682—C694. http://dx.doi.org/10.1152/ajpcell.00101.2010.

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The melastatin (M) transient receptor potential (TRP) channel TRPM4 mediates pressure and protein kinase C (PKC)-induced smooth muscle cell depolarization and vasoconstriction of cerebral arteries. We hypothesized that PKC causes vasoconstriction by stimulating translocation of TRPM4 to the plasma membrane. Live-cell confocal imaging and fluorescence recovery after photobleaching (FRAP) analysis was performed using a green fluorescent protein (GFP)-tagged TRPM4 (TRPM4-GFP) construct expressed in A7r5 cells. The surface channel was mobile, demonstrating a FRAP time constant of 168 ± 19 s. In ad
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Martin, M. A., W. M. Nauseef, and R. A. Clark. "Depolarization blunts the oxidative burst of human neutrophils. Parallel effects of monoclonal antibodies, depolarizing buffers, and glycolytic inhibitors." Journal of Immunology 140, no. 11 (1988): 3928–35. http://dx.doi.org/10.4049/jimmunol.140.11.3928.

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Abstract The anti-neutrophil mAb PMN 7C3 and IIC4 inhibited the respiratory burst of neutrophils as measured by the generation of superoxide anion or hydrogen peroxide in response to PMA, serum-treated zymosan, and FMLP. To examine the effect of these mAb on neutrophil transmembrane potential, a fluorescent probe was used in a continuous assay. Compared with control cells, antibody-treated neutrophils were partially depolarized at rest and had a blunted response when stimulated. The F(ab)2 fragment of PMN 7C3 had similar effects on both the respiratory burst and transmembrane potential, wherea
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Zhang, Yongli, Xiangsheng Wang, Wei Fang, et al. "Synthesis andIn VitroAntitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline." Bioinorganic Chemistry and Applications 2013 (2013): 1–14. http://dx.doi.org/10.1155/2013/437134.

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Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS,1H NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Ann
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Huang, Shan, Jing Huang, Jingyun Du, et al. "The LiaSR Two-Component System Regulates Resistance to Chlorhexidine in Streptococcus mutans." Microorganisms 12, no. 3 (2024): 468. http://dx.doi.org/10.3390/microorganisms12030468.

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Chlorhexidine (CHX) is widely considered to be the gold standard for preventing dental caries. However, it is possible to induce resistance to CHX. The LiaSR two-component system has been identified that contributed to CHX resistance in Streptococcus mutans, which is one of the major pathogens in dental caries. However, the underlying mechanisms remain unclear. In this study, an MIC assay and a viability assessment demonstrated that after deleting the liaS and liaR genes, the sensitivity of mutants could increase. The Nile Red efflux assay exhibited that the efflux rates of mutants were signif
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Dissertations / Theses on the topic "Single-cell membrane depolarization assay"

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VERZE', Genny. "CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (CFTR) IN HUMAN LEUKOCYTES." Doctoral thesis, 2013. http://hdl.handle.net/11562/551349.

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SCOPO: 1) valutare l’espressione della proteine che regola il trasporto del cloro transmembrana (CFTR) e la sua attività funzionale nei monociti; 2) creare delle linee cellulari immortalizzate a partire da linfociti B aventi genotipi differenti; 3) valutare linee cellulari immortalizzate. CONOSCENZE DI BASE: La Fibrosi Cistica (CF), la più commune e grave malattia autosomale diffusa nei Paesi Caucasici, è dovuta alla presenza di mutazioni sul gene CFTR. Sebbene la CF sia una malattia multi organo, la patologia polmonare è la principale causa di morte tra i pazienti CF. E’ caratterizzata da una
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Book chapters on the topic "Single-cell membrane depolarization assay"

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Thiel*, Gerhard, Jens-Uwe Sutter,, and Ulrike Homann*. "Electrophysiological methods: monitoring exo- and endocytosis in real time." In Plant Cell Biology. Oxford University PressOxford, 2001. http://dx.doi.org/10.1093/oso/9780199638666.003.0008.

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Abstract About two decades ago Neher and Marty (1) showed that the patch-clamp technique in combination with some impedance analysis could be used for monitoring the membrane capacitance as a quasi real time assay for exocytosis and endocytosis. This method allows recording of exo- and endocytosis in single animal cells in vivo with such a high resolution, that single exo- and endocytotic events from vesicles with a diameter under 100 nm can be recorded with a temporal resolution in the order of some 10 msec. This method has been adopted with success in plant physiology laboratories to examine
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Weston, Matthew C., and Anastasios V. Tzingounis. "Potassium Channels in Genetic Epilepsy." In Jasper's Basic Mechanisms of the Epilepsies, 5th ed., edited by Jeffrey L. Noebels. Oxford University PressNew York, 2024. http://dx.doi.org/10.1093/med/9780197549469.003.0045.

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Abstract The past 70 years have provided a foundation for understanding potassium channel function at the single-channel, cellular, and circuit levels. Basic science of epilepsy has implicated potassium channel dysfunction in the pathogenesis of acquired epilepsy, and, more recently, genetic studies have revealed a large number of variants in potassium channel–encoding genes that cause epilepsy in humans. Identification and functional assessment of these variants have demonstrated they alter protein function, generating several hypotheses for how potassium channel dysfunction leads to the hype
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Conference papers on the topic "Single-cell membrane depolarization assay"

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Colman, R. W., A. Gewirtz, D. L. Wang, et al. "BIOSYNTHESIS AND EXPRESSION OF FACTOR V IN MAGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642955.

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Coagulation factor V (FV), is a single chain, multifunctional glycoprotein of Mr 350,000 which interacts with a variety of hemostatic proteins such as factor Xa, prothrombin, thrombin and protein C, on the surface of platelets and vascular endothelial cells. FV serves as both a cofactor and substrate in the generation of thrombin and plays a critical regulatory role in both physiologic hemostasis and pathologic thrombosis. The biosynthesis of FV and its subsequent expression are therefore expected to be precisely controlled and may differ in the three sites of synthesis - hepatocytes, endothel
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Reports on the topic "Single-cell membrane depolarization assay"

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Thanyasrisung, Panida, Aemvika Vittayaprasit, Voravee Hoven, Sugai, Motoyuki, and Oranart Matangkasombut. Rapid detection of mutans streptococci by substrate specific binding of automutanolysin : Final report. Faculty of Dentistry, Chulalongkorn University, 2016. https://doi.org/10.58837/chula.res.2016.19.

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Chair-side rapid detection of mutans streptococci is an important aid to clinical dental caries risk assessment. Rapid Streptococcus mutans detection tools are available on the market but there are a small number. Automutanolysin (Aml) is a peptidoglycan hydrolase whose cell wall-binding domain (CWBD) has substrate-specificity towards mutans streptococci. This study aims to develop a rapid detection assay using CWBD conjugated with horseradish peroxidase (HRP). However, the recombinant protein was as insoluble form. Therefore, magnetic nanoparticles were used as an alternative reporter to conj
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