Relevant bibliographies by topics / Single cell mRNA sequencing

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  1. Dissertations / Theses

Academic literature on the topic 'Single cell mRNA sequencing'

Author: Grafiati
Published: 25 January 2025

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Dissertations / Theses on the topic "Single cell mRNA sequencing"

1

Johnson, Travis Steele. "Integrative approaches to single cell RNA sequencing analysis." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1586960661272666.

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2

Borgström, Erik. "Technologies for Single Cell Genome Analysis." Doctoral thesis, KTH, Genteknologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-181059.

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During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. The four papers included in this thesis describe or evaluate technological advancements for high throughput DNA sequencing of single cells and single molecules. As the sequencing technologies improve, more samples are analyzed in parallel. In paper 1, an automated procedure for preparation of samples prior to massively parallel sequen
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La, Forest Divonne Sébastien De. "Caractérisation constitutive et en condition d'infection bactérienne des populations hémocytaires par une approche intégrative cytologiques, transcriptomiques et fonctionnelles chez l'huitre creuse Crassostrea (Magallana) gigas." Electronic Thesis or Diss., Perpignan, 2024. http://www.theses.fr/2024PERP0025.

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L'huître du Pacifique, Crassostrea (Magallana) gigas, est un mollusque bivalve d'une importance écologique et économique majeure, et constitue désormais une espèce modèle émergente dans l'étude de l'immunité innée des bivalves. Ces dernières années, les élevages d'huîtres ont été confrontés à des épisodes de mortalités massives, exacerbés par le changement climatique et les activités anthropiques. Ces mortalités, bien que multifactorielles, partagent un point commun : la capacité des pathogènes (virus et bactéries) à échapper aux défenses immunitaires des huîtres, entraînant des septicémies so
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Raoux, Corentin. "Review and Analysis of single-cell RNA sequencing cell-type identification and annotation tools." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-297852.

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Single-cell RNA-sequencing makes possible to study the gene expression at the level of individual cells. However, one of the main challenges of the single-cell RNA-sequencing analysis today, is the identification and annotation of cell types. The current method consists in manually checking the expression of genes using top differentially expressed genes and comparing them with related cell-type markers available in scientific publications. It is therefore time-consuming and labour intensive. Nevertheless, in the last two years,numerous automatic cell-type identification and annotation tools w
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Kindblom, Marie, and Hakim Ezeddin Al. "Phylogenetic fatemapping: estimating allelic dropout probability in single cell genomic sequencing." Thesis, KTH, Skolan för datavetenskap och kommunikation (CSC), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-186453.

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Single-cell genomic sequencing is a rapidly developing field that will play a vital role in human biology and science in the future. As of now, next-generation sequencing is accelerating in speed and decreasing in cost more quickly than Moore's law. Studies have shown that all cells in the human body have with very high probability a unique genomic signature, due to the somatic evolution which have accumulated mutations starting from the zygotic state. The possible reconstruction of phylogenetic lineage trees would be of vital importance to several fields in medicine, such as the stem cell res
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Henao, Diaz Emanuela. "Towards single-cell exome sequencing with spatial resolution in tissue sections." Thesis, KTH, Skolan för bioteknologi (BIO), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-150564.

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7

Evrony, Gilad David. "Single-cell Sequencing Studies of Somatic Mutation in the Human Brain." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:10747.

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A major unanswered question in neuroscience is whether there exists genomic variability between individual neurons of the brain, contributing to functional diversity or to an unexplained burden of neurologic disease. To address this question, we developed methods to amplify genomes of single neurons from human brains, achieving >80% genome coverage of single-cells and allowing study of a wide-range of somatic mutation types.
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8

Ke, Rongqin. "Detection and Sequencing of Amplified Single Molecules." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-183141.

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Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). ASMs were generated from padlock probe assay and proximity ligation assay (PLA) through a series of molecular processes. In Paper I, a simple colorimetric readout strategy for detection of ASMs generated from padlock probe assay was used for highly sensitive detection of RNA virus, showing the potential of using padlock pro
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9

Tu, Ang A. (Ang Andy). "Recovery of T cell receptor variable sequences from 3' barcoded single-cell RNA sequencing libraries." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/127888.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, May, 2020<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 107-112).<br>Heterogeneity of the immune system has increasingly necessitated the use of high-resolution techniques, including flow cytometry, RNA-seq, and mass spectrometry, to decipher the immune underpinnings of various diseases such as cancer and autoimmune disorders. In recent years, high-throughput single-cell RNA sequencing (scRNA-seq) has gained popularity among immunologists due to its ability to e
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10

Lefebvre, Keely. "Resolving the Taxonomy and Phylogenetics of Benthic Diatoms from Single Cell Sequencing." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/34553.

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Benthic diatoms are often used as indicators of water quality and past environmental conditions. This depends entirely on a reliable taxonomic system. With the advent of DNA techniques, genetic analyses can now be used in tandem with traditional microscopy in order to improve taxonomy and determine evolutionary relationships. This thesis examined a speciose genus of diatoms Neidium (> 300 species) and, using sequence data from molecular markers as well as traditional morphological analyses, investigated phylogenetic relationships. Fresh benthic samples from aquatic ecosystems in Eastern North
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