Log in

Relevant bibliographies by topics / Single-cell RNA / Dissertations / Theses

To see the other types of publications on this topic, follow the link: Single-cell RNA.

Dissertations / Theses on the topic 'Single-cell RNA'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 dissertations / theses for your research on the topic 'Single-cell RNA.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.

1

Johnson, Travis Steele. "Integrative approaches to single cell RNA sequencing analysis." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1586960661272666.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Mahi, Naim. "Connectivity Analysis of Single-cell RNA-seq Derived Transcriptional Signatures." University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1613748441148963.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Raoux, Corentin. "Review and Analysis of single-cell RNA sequencing cell-type identification and annotation tools." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-297852.

Full text

Abstract:

Single-cell RNA-sequencing makes possible to study the gene expression at the level of individual cells. However, one of the main challenges of the single-cell RNA-sequencing analysis today, is the identification and annotation of cell types. The current method consists in manually checking the expression of genes using top differentially expressed genes and comparing them with related cell-type markers available in scientific publications. It is therefore time-consuming and labour intensive. Nevertheless, in the last two years,numerous automatic cell-type identification and annotation tools w

APA, Harvard, Vancouver, ISO, and other styles

4

A, Ahern Megan, Black Claudine P, Seedorf Gregory J, Baker Christopher D, and Shepherd Douglas P. "Hyperoxia impairs pro-angiogenic RNA production in preterm endothelial colony-forming cells." AMER INST MATHEMATICAL SCIENCES-AIMS, 2017. http://hdl.handle.net/10150/626103.

Full text

Abstract:

Disruptions in the response of endothelial progenitor cells to changes in oxygen environment may present a possible mechanism behind multiple pediatric pulmonary disease models, such as bronchopulmonary dysplasia. Using high-throughput fixed single-cell protein and RNA imaging, we have created "stop-motion" movies of Thymosin. 4 (T beta 4) and Hypoxia Inducible Factor 1 alpha (HIF-1 alpha) protein expression and vascular endothelial growth factor (vegf) and endothelial nitric oxide synthase (eNOS) mRNA in human umbilical cord-derived endothelial colony-forming cells (ECFC). ECFC were grown in

APA, Harvard, Vancouver, ISO, and other styles

5

Carlberg, Konstantin. "In Situ RNA Quality Control : A spatial heat map of RNA integrity with single cell resolution." Thesis, KTH, Skolan för bioteknologi (BIO), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-176873.

Full text

Abstract:

The quality of RNA is of great importance in gene expression studies. It is mostly measured using the RNA integrity number (RIN). Lately it has been shown that samples with low RIN and different fragmentation patterns could affect quality of sequencing data. For such low RIN samples a new approach has been developed by Illumina called the DV200 metric, which is the percentage of fragments >200 nucleotides. For samples with low RIN, DV200 has proved to be a better method to predict if good quality data from RNA sequencing can be generated. However, neither RIN nor DV200 provide spatial infro

APA, Harvard, Vancouver, ISO, and other styles

6

Tu, Ang A. (Ang Andy). "Recovery of T cell receptor variable sequences from 3' barcoded single-cell RNA sequencing libraries." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/127888.

Full text

Abstract:

Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, May, 2020<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 107-112).<br>Heterogeneity of the immune system has increasingly necessitated the use of high-resolution techniques, including flow cytometry, RNA-seq, and mass spectrometry, to decipher the immune underpinnings of various diseases such as cancer and autoimmune disorders. In recent years, high-throughput single-cell RNA sequencing (scRNA-seq) has gained popularity among immunologists due to its ability to e

APA, Harvard, Vancouver, ISO, and other styles

7

Ziegenhain, Christoph [Verfasser], and Wolfgang [Akademischer Betreuer] Enard. "Improving & applying single-cell RNA sequencing / Christoph Ziegenhain ; Betreuer: Wolfgang Enard." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1151818372/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Vieth, Beate [Verfasser], and Wolfgang [Akademischer Betreuer] Enard. "Statistical power analysis for single-cell RNA-sequencing / Beate Vieth ; Betreuer: Wolfgang Enard." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/1225683033/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Chwastek, Damian. "Elucidating the Contribution of Stroke-Induced Changes to Neural Stem and Progenitor Cells Associated with a Neuronal Fate." Thesis, Université d'Ottawa / University of Ottawa, 2021. http://hdl.handle.net/10393/41839.

Full text

Abstract:

Following stroke there is a robust increase in the proliferation of neural stem and progenitor cells (NSPCs) that ectopically migrate from the subventricular zone (SVZ) to surround the site of damage induced by stroke (infarct). Previous in vivo studies by our lab and others have shown that a majority of migrating NSPCs when labelled prior to stroke become astrocytes surrounding the infarct. In contrast, our lab has shown that the majority of NSPCs when labelled after stroke become neurons surrounding the infarct. This thesis aims to elucidate the contributions of intrinsic changes that can al

APA, Harvard, Vancouver, ISO, and other styles

10

Svensson, Valentine. "Probabilistic modelling of cellular development from single-cell gene expression." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/267937.

Full text

Abstract:

The recent technology of single-cell RNA sequencing can be used to investigate molecular, transcriptional, changes in cells as they develop. I reviewed the literature on the technology, and made a large scale quantitative comparison of the different implementations of single cell RNA sequencing to identify their technical limitations. I investigate how to model transcriptional changes during cellular development. The general forms of expression changes with respect to development leads to nonparametric regression models, in the forms of Gaussian Processes. I used Gaussian process models to inv

APA, Harvard, Vancouver, ISO, and other styles

11

Teufel, Lotte. "Cyclins and their roles in cell cycle progression, transcriptional regulation and osmostress adaptation in Saccharomyces cerevisiae. A transcriptome-wide and single cell approach." Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21205.

Full text

Abstract:

Der eukaryotische Zellzyklus ist ein streng regulierter Prozess, für dessen zeitlichen Ablauf unter anderem oszillierende Genexpression notwendig ist. Die Regulation und die zeitliche Koordination des Zellzyklus sind nach wie vor fundamentale Fragen der Zellbiologie. Spezifische Ereignisse, wie DNA Replikation und Zellkernteilung, können vier Zellzyklusphasen zugeordnet werden, welche durch Cyclin-abhängige Kinasen, Cycline und deren Inhibitoren reguliert werden. Während in Saccharomyces cerevisiae Cyclin-abhängige Kinasen (Cdc28, Pho85) über den gesamten Zellzyklus zu Verfügung stehen, wer

APA, Harvard, Vancouver, ISO, and other styles

12

MAHMOUD, NADY ABDELMOEZ ATTA. "On-chip Electrophoretic Fractionation of Cytoplasmic and Nuclear RNA from Single Cells." Kyoto University, 2019. http://hdl.handle.net/2433/244546.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Kinchen, James. "Intestinal stromal cell types in health and inflammatory bowel disease uncovered by single-cell transcriptomics." Thesis, University of Oxford, 2017. http://ora.ox.ac.uk/objects/uuid:1bf9d8f0-6d09-46f5-9d1e-3c9e0b826618.

Full text

Abstract:

Colonic stromal cells provide critical structural support but also regulate immunity, tolerance and inflammatory responses in the mucosa. Substantial variability and plasticity of mucosal stromal cells has been reported but a paucity of distinct marker genes exist to identify distinct cell states. Here single-cell RNA-sequencing is used to document heterogeneity and subtype specific markers of individual colonic stromal cells in human and mouse. Marker-free transcriptional clustering of fibroblast-like cells derived from healthy human tissue reveals distinct populations corresponding to myofib

APA, Harvard, Vancouver, ISO, and other styles

14

Reddy, Veena K. "Analysis of single cell RNA seq data to identify markers for subtyping of non-small cell lung cancer." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-18514.

Full text

Abstract:

Single cell RNA technology is a recent technical advancement used to understand the cancertumorgenicity at single cell resolution. In this study we have analyzed the scRNA data from thenon-small cell lung cancer (NSCLC) dataset to facilitate the early identification of NSCLCsubtypes namely, squamous cell carcinoma (SCC) and adenocarcinoma (AC). Non-immunecells, have a major role in tumorigenesis of the malignant tumors, in early stages. Therefore,we have analyzed the major non-immune cells, namely endothelial cells and fibroblast cellsfrom the GSE127465 dataset using SEURAT pipeline. Dimension

APA, Harvard, Vancouver, ISO, and other styles

15

AZZALINI, EROS. "Comprehensive characterization and effective combinatorial targeting of high-grade serous ovarian cancer via single-cell analysis." Doctoral thesis, Università degli Studi di Trieste, 2020. http://hdl.handle.net/11368/2967979.

Full text

Abstract:

Ovarian cancer kills more than 40 000 women in Europe and more than 150 000 women globally each year. High-grade serous ovarian cancer (HGSOC) is the most common and most difficult to treat subtype of the disease. The high-grade serous tumors are highly heterogeneous, therefore, though most of the patients respond well to surgery and chemotherapy initially, more than half experience relapse. HERCULES is a research project funded by the EU H2020 program with the target to characterize comprehensively high grade serous ovarian cancers to find novel therapeutic strategies to fight them. The aim o

APA, Harvard, Vancouver, ISO, and other styles

16

Cavard, Amélie. "Contribution des gènes des loci 5q11 et 11q23 à la multiciliogenèse et régulation de l’équilibre mucociliaire de l’épithélium des voies respiratoires au cours de la régénération in vitro." Electronic Thesis or Diss., Université Côte d'Azur, 2020. http://www.theses.fr/2020COAZ6006.

Full text

Abstract:

Les voies respiratoires sont bordées par un épithélium mucocilié constitué majoritairement de cellules basales, de cellules à mucus et de cellules multiciliées portant des centaines de cils motiles à leur pôle apical. Leur battement coordonné permet d’expulser les particules inhalées piégées dans le mucus. En cas de lésions, l’épithélium a la capacité de se régénérer à l’identique en maintenant un équilibre strict entre les proportions de chaque type cellulaire. Dans les maladies respiratoires chroniques comme la mucoviscidose, les inflammations et infections chroniques provoquent un remodelag

APA, Harvard, Vancouver, ISO, and other styles

17

Lee, Jiyoung. "Computational Analysis of Gene Expression Regulation from Cross Species Comparison to Single Cell Resolution." Diss., Virginia Tech, 2020. http://hdl.handle.net/10919/99878.

Full text

Abstract:

Gene expression regulation is dynamic and specific to various factors such as developmental stages, environmental conditions, and stimulation of pathogens. Nowadays, a tremendous amount of transcriptome data sets are available from diverse species. This trend enables us to perform comparative transcriptome analysis that identifies conserved or diverged gene expression responses across species using transcriptome data. The goal of this dissertation is to develop and apply approaches of comparative transcriptomics to transfer knowledge from model species to non-model species with the hope that s

APA, Harvard, Vancouver, ISO, and other styles

18

Bost, Pierre. "Decoding cellular communications and interactions between immune cells by using single-cell approaches." Electronic Thesis or Diss., Sorbonne université, 2020. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2020SORUS020.pdf.

Full text

Abstract:

Les communications cellulaires sont indispensables au bon fonctionnement des organismes multicellulaires, notamment pour s’adapter à un environnement changeant en permanence. Les cellules du système immunitaire n’échappent pas à cette règle mais les interactions entre cellules immunitaires restent peu connues et compliquée à étudier. La récente apparition des technologies de séquençage dites ‘cellules uniques’ représente une opportunité unique pour étudier ces communications. Dans cette thèse, différentes approches expérimentales et analytiques ont été développées pour étudier ces communicatio

APA, Harvard, Vancouver, ISO, and other styles

19

MELDGAARD, TRINE. "New technology platforms applied to the profiling of gene and protein expression at the single T-cell level." Doctoral thesis, Københavns Universitet, 2016. http://hdl.handle.net/11365/1004589.

Full text

Abstract:

Priming of antigen-specific CD8+ T cells by vaccination is key for subsequent viral clearance. However, to date, CD8+ T-cell characterization is performed under the assumption that cells of a particular type are identical, and not acknowledging their heterogeneous nature. It has recently been proven, in many different studies, that cells do differ quite significantly and can drive different responses. In order to characterize the heterogeneity of vaccine-induced CD8+ T cells, we performed gene expression profiling and protein characterization at the single-cell level, after delivery of

APA, Harvard, Vancouver, ISO, and other styles

20

Hu, Bo. "Analysis of cellular drivers of zebrafish heart regeneration by single-cell RNA sequencing and high-throughput lineage tracing." Doctoral thesis, Humboldt-Universität zu Berlin, 2021. http://dx.doi.org/10.18452/23324.

Full text

Abstract:

Das Herz eines Zebrafishs ist bemerkenswert, da es sich nach einer Verletzung vollständig regenerieren kann. Der Regenerationsprozess wird von Fibrose begleitet - der Bildung von überschüssigem Gewebe der extrazellulären Matrix (ECM). Anders als bei Säugetieren ist die Fibrose im Zebrafish nur transient. Viele Signalwege wurden identifiziert, die an der Herzregeneration beteiligt sind. Allerdings sind die Zelltypen, insbesondere Nicht-Kardiomyozyten, die für die Regulation des Regenerationsprozesses verantwortlich sind, weitgehend unbekannt. In dieser Arbeit haben wir systematisch alle Zelltyp

APA, Harvard, Vancouver, ISO, and other styles

21

Truchi, Marin. "Le séquençage de transcrits sur cellule unique appliqué à l'étude des mécanismes de formation et de résolution de la fibrose pulmonaire." Electronic Thesis or Diss., Université Côte d'Azur, 2023. http://www.theses.fr/2023COAZ6016.

Full text

Abstract:

Le rétablissement de l'intégrité du tissu pulmonaire en réponse aux blessures dépend notamment des capacités régénératives des cellules endothéliales capillaires (CEc), susceptibles de décliner au cours du vieillissement. Des réponses inadaptées peuvent entraîner le développement de pathologies chroniques telles que la fibrose pulmonaire idiopathique (FPI), qui se caractérise par l'accumulation de matrice extracellulaire et un remodelage de l'environnement alvéolaire, menant à une destruction progressive du parenchyme et aboutissant à une insuffisance respiratoire. Chez la souris, des lésions

APA, Harvard, Vancouver, ISO, and other styles

22

Magella, Bliss. "A Journey Through the Developing Kidney:Analysis of normal and Hoxa9,10,11-/-Hoxd9,10,11-/- Mouse Models." University of Cincinnati / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1523629396626027.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Rudomanova, Valeriia. "Unraveling the Secrets of Kidney Disease: Novel Molecular Mechanisms of Acute and Chronic Kidney Injury." University of Cincinnati / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1623250686588821.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Vuong, Nhung. "Molecular Mechanisms by Which Estrogen Causes Ovarian Epithelial Cell Dysplasia." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/37286.

Full text

Abstract:

The initiating events of ovarian cancer remain unknown, but an established risk factor is use of estrogen therapy by post-menopausal women where there is a positive correlation between duration of use and risk for disease. Mouse models of ovarian cancer have shown that exposure to exogenous 17β-estradiol (E2) accelerates tumour onset so this study aims to investigate the E2 signalling mechanisms responsible for sensitizing ovarian epithelial cells to transformation. By developing model systems that are responsive to E2 manipulation, we showed that E2 induces the formation of epithelial dysplas

APA, Harvard, Vancouver, ISO, and other styles

25

Tatsuoka, Hisato. "Single-cell Transcriptome Analysis Dissects the Replicating Process of Pancreatic Beta Cells in Partial Pancreatectomy Model." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263543.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Muench, David. "Gfi1-controlled transcriptional circuits in normal and malignant hematopoiesis." University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1553250015825734.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Hu, Bo [Verfasser]. "Analysis of cellular drivers of zebrafish heart regeneration by single-cell RNA sequencing and high-throughput lineage tracing / Bo Hu." Berlin : Humboldt-Universität zu Berlin, 2021. http://nbn-resolving.de/urn:nbn:de:kobv:11-110-18452/24021-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

LAMBROIA, LUCA. "Single-cell RNA analysis to investigate the role of phenotypic modulation of smooth muscle cells in the onset of atherosclerosis." Doctoral thesis, Università degli studi di Brescia, 2023. https://hdl.handle.net/11379/570185.

Full text

Abstract:

Motivazione: l'aterosclerosi è una malattia infiammatoria cronica responsabile di quasi il 50% di tutti i decessi nei paesi occidentali. È caratterizzata dalla formazione di placche lipidiche nella parete arteriosa che riducono il lume, diminuiscono l'elasticità delle pareti e provocano molte malattie cardiovascolari. In risposta a segnali ambientali, le cellule muscolari lisce (SMC) dei vasi possono de-differenziarsi, proliferare e migrare in un processo noto come modulazione fenotipica. Recentemente è stato dimostrato che possono transdifferenziarsi in altri tipi cellulari che sono important

APA, Harvard, Vancouver, ISO, and other styles

29

Redman, Elisa. "Découverte des mécanismes responsables des défauts de régénération épithéliale dans l'asthme, par approche transcriptomique sur cellules uniques." Electronic Thesis or Diss., Université Côte d'Azur, 2024. http://www.theses.fr/2024COAZ6017.

Full text

Abstract:

L'épithélium des voies respiratoires est un écosystème complexe composé de cellules basales, de cellules à gobelet et de cellules multiciliées (MCC), ainsi que d'autres types cellulaires plus rares. Les cils motiles des MCC permettent d'éliminer le mucus piégeur d'agents pathogènes produit par les cellules à gobelet. Dans l'asthme, l'épithélium subit une inflammation chronique et un remodelage, menant à une diminution du nombre de MCC et à une hyperplasie des cellules à gobelet, ce qui altère la clairance mucociliaire et aggrave les symptômes. L'interleukine 13 (IL-13), produite par les lympho

APA, Harvard, Vancouver, ISO, and other styles

30

Ferraioli, Anna. "Comparison of cell types across life cycle stages of the hydrozoan Clytia hemisphaerica." Electronic Thesis or Diss., Sorbonne université, 2022. http://www.theses.fr/2022SORUS497.

Full text

Abstract:

L'Hydrozoaire Clytia hemisphaerica présente un cycle de vie triphasique, typique des hydrozoaires, comprenant une colonie de polypes à propagation végétative et une forme nageant librement, la méduse qui est se reproduisent de façon sexuée. Les méduses mâles et femelles se reproduisent quotidiennement, déclenchées par la lumière et environ un jour après la fécondation, une larve planula ciliée se forme. Après trois jours, la planula se fixe sur le substrat et se métamorphose pour donner naissance à un polype fondateur de la colonie, destiné pour l’alimentation, le gastrozooïde. La colonie se p

APA, Harvard, Vancouver, ISO, and other styles

31

Choudry, Fizzah Aziz. "Novel insights into megakaryopoiesis, thrombopoiesis and acute coronary thrombosis : transcriptome profiling of the haematopoietic stem cell, megakaryocyte and platelet." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/283252.

Full text

Abstract:

The aim of this project was to investigate the transcriptome of human haematopoietic stem cells (HSCs), megakaryocytes and platelets to gain insights into steady state and accelerated thrombopoiesis that occurs in states of haemostatic demand and in thrombosis by applying these findings to the pathological setting of acute coronary thrombosis. To investigate transcriptional heterogeneity within the human HSC population, single cell RNA sequencing was performed in human bone marrow HSCs. Transcriptionally distinct subpopulations were identified including two megakaryocyte biased subsets with po

APA, Harvard, Vancouver, ISO, and other styles

32

Petrany, Michael J. "Consequences of Cell Fusion and Multinucleation for Skeletal Muscle Development and Disease." University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595847866440328.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Kuut, Gunnar [Verfasser], and Veit [Akademischer Betreuer] Hornung. "Using RNA barcoding and sequencing to study cellular differentiation on a single-cell and population level / Gunnar Kuut ; Betreuer: Veit Hornung." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2021. http://d-nb.info/123801707X/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Linnik, Volha. "Functional analysis of a plant virus replication 'factory' using live cell imaging." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4639.

Full text

Abstract:

Plant viruses have developed a number of strategies that enable them to become obligate intracellular parasites of many agricultural crops. Potato virus X (PVX) belongs to a group of positive-sense, single-stranded plant RNA viruses that replicate on host membranes and form elaborate structures known as viral replication complexes (VRCs) that contain viral RNA (vRNA), proteins and host cellular components. VRCs are the principal sites of viral genome replication, virion assembly and packaging of vRNA for export into neighbouring cells. For many animal viruses, host membrane association is cruc

APA, Harvard, Vancouver, ISO, and other styles

35

Genga, Ryan M. "Towards Understanding the Molecular Basis of Human Endoderm Development Using CRISPR-Effector and Single-Cell Technologies." eScholarship@UMMS, 2019. https://escholarship.umassmed.edu/gsbs_diss/1008.

Full text

Abstract:

The definitive endoderm gives rise to several specialized organs, including the thymus. Improper development of the definite endoderm or its derivatives can lead to human disease; in the case of the thymus, immunodeficiency or autoimmune disorders. Human pluripotent stem cells (hPSCs) have emerged as a system to model human development, as study of their differentiation allows for elucidation of the molecular basis of cell fate decisions, under both healthy and impaired conditions. Here, we first developed a CRISPR-effector system to control endogenous gene expression in hPSCs, a novel approac

APA, Harvard, Vancouver, ISO, and other styles

36

Hirabayashi, Shigeki. "APOBEC3B is preferentially expressed at the G2/M phase of cell cycle." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/264663.

Full text

Abstract:

京都大学<br>新制・課程博士<br>博士(医学)<br>甲第23382号<br>医博第4751号<br>新制||医||1052(附属図書館)<br>京都大学大学院医学研究科医学専攻<br>(主査)教授伊藤貴浩, 教授滝田順子, 教授江藤浩之<br>学位規則第4条第1項該当<br>Doctor of Medical Science<br>Kyoto University<br>DFAM

APA, Harvard, Vancouver, ISO, and other styles

37

Lavagi, Ilaria Verfasser], and Eckhard [Akademischer Betreuer] [Wolf. "Analysis of blastomere of bovine embryos during genome activation by evaluation of single-cell RNA sequencing data / Ilaria Lavagi ; Betreuer: Eckhard Wolf." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/1167160541/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Krzywkowski, Tomasz. "iLocks: a novel tool for RNA assays with improved specificity." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-147734.

Full text

Abstract:

The Central Dogma of molecular biology describes a framework for how genetic information is transferred in cells, placing RNA as a messenger between DNA and translated proteins. During the last years, interest in RNA research has grown tremendously due to the increasing understanding and recognition of the importance of RNA in regulation of gene expression, biochemical catalysis, and genome integrity surveillance. Most importantly, RNA content, unlike DNA, changes constantly, fine-tuning the cellular response to match the environmental conditions. There is a clear potential for RNA biomarkers,

APA, Harvard, Vancouver, ISO, and other styles

39

Wu, Zhiyuan [Verfasser], Lars [Akademischer Betreuer] Mägdefessel, Hendrik [Gutachter] Sager, and Lars [Gutachter] Mägdefessel. "Single-Cell RNA Sequencing Analysis Revealed Cellular Heterogeneity of Human Abdominal Aortic Aneurysm / Zhiyuan Wu ; Gutachter: Hendrik Sager, Lars Mägdefessel ; Betreuer: Lars Mägdefessel." München : Universitätsbibliothek der TU München, 2021. http://d-nb.info/1240384114/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Puram, Rishi Venkata. "Defining and Targeting Transcriptional Pathways in Leukemia Stem Cells." Thesis, Harvard University, 2014. http://nrs.harvard.edu/urn-3:HUL.InstRepos:13070042.

Full text

Abstract:

Acute myeloid leukemia (AML) is a clonal neoplastic disorder organized as a cellular hierarchy, with the self-renewing leukemia stem cell (LSC) at the apex. Recurrent mutations in transcription factors (TF) and epigenetic regulators suggest that AML is driven by aberrant transcriptional circuits, but these circuits have not been fully defined in an LSC model. To study transcriptional mechanisms relevant to leukemogenesis in vivo, we generated a murine serial transplantation model of MLL-AF9-driven, myelomonocytic leukemia with genetically- and phenotypically-defined LSCs. Using this model,

APA, Harvard, Vancouver, ISO, and other styles

41

Ernst, Christina. "Transcriptional and developmental consequences of aneuploidy during male meiosis." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/278212.

Full text

Abstract:

Eukaryotes have developed stringent regulatory mechanisms that control cell division and ensure proper chromosome segregation. Maintaining genome integrity is especially important during meiosis, the specialised cell division programme in the germline that generates haploid gametes. As these cells transmit genetic information to the next generation, the consequences of meiotic errors are not restricted to an organismal level, but can directly impact the fitness of the offspring. Mammals display a high degree of sexual dimorphism in meiosis with regard to the stringency of regulatory mechanisms

APA, Harvard, Vancouver, ISO, and other styles

42

Chen, Huiyi. "System-Wide Studies of Gene Expression in Escherichia coli by Fluorescence Microscopy and High Throughput Sequencing." Thesis, Harvard University, 2011. http://dissertations.umi.com/gsas.harvard:10044.

Full text

Abstract:

Gene expression is a fundamental process in the cell and is made up of two parts – the information flow from DNA to RNA, and from RNA to protein. Here, we examined specific sub-processes in Escherichia coli gene expression using newly available tools that permit genome-wide analysis. We begin our studies measuring mRNA and protein abundances in single cells by single-molecule fluorescence microscopy, and then focus our attention to studying RNA generation and degradation by high throughput sequencing. The details of the dynamics of gene expression can be observed from fluctuations in mRNA and

APA, Harvard, Vancouver, ISO, and other styles

43

Sarma, Mimosa. "Microfluidic platforms for Transcriptomics and Epigenomics." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/90294.

Full text

Abstract:

A cell, the building block of all life, stores a plethora of information in its genome, epigenome, and transcriptome which needs to be analyzed via various Omic studies. The heterogeneity in a seemingly similar group of cells is an important factor to consider and it could lead us to better understand processes such as cancer development and resistance to treatment, fetal development, and immune response. There is an ever growing demand to be able to develop more sensitive, accurate and robust ways to study Omic information and to analyze subtle biological variation between samples even with l

APA, Harvard, Vancouver, ISO, and other styles

44

GERVASONI, FEDERICA. "DEVELOPMENT OF BIOINFORMATIC METHODS FOR THE INTEGRATION OF TRANSCRIPTOMIC AND EPIGENOMIC ANALYSIS OF COLORECTAL CANCER DERIVED ORGANOIDS." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/810878.

Full text

Abstract:

Cancer is characterized by pervasive epigenetic alterations with enhancer dysfunction as a key driver of tumor transcriptional deregulation and dependencies. In this work, we seek to unravel the chromatin landscape of human colorectal cancer (CRC) by exploiting the organoid model in order to identify a common epigenetic blueprint and investigate its relevance in other types of cancers. To this extent, we generated a library of patient-derived organoids (PDOs) from different subtypes of CRC representing the heterogeneity of this type of tumor. We reconstructed the epigenetic landscape of CRC a

APA, Harvard, Vancouver, ISO, and other styles

45

Fredriksson, Mona. "Using Minisequencing Technology for Analysing Genetic Variation in DNA and RNA." Doctoral thesis, Uppsala University, Department of Medical Sciences, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4789.

Full text

Abstract:

<p>In this thesis, the four-color fluorescence tag-microarray minisequencing system pioneered by our group was further developed and applied for analysing genetic variation in human DNA and RNA. A SNP marker panel representing different chromosomal regions was established and used for identification of informative SNP markers for monitoring chimerism after stem cell transplantation (SCT). The success of SCT was monitored by measuring the allelic ratios of informative SNPs in follow-up samples from nine patients with leukaemia. The results agreed with data obtained using microsatellite markers.

APA, Harvard, Vancouver, ISO, and other styles

46

Kaganovich, Alice. "Contribution of microglia to the pathogenic mechanisms behind LRRK2-associated Parkinson's disease." Doctoral thesis, Università degli studi di Padova, 2019. http://hdl.handle.net/11577/3422816.

Full text

Abstract:

Microglia are immune cells of the brain playing critical roles during the inflammatory response. Among the genes mutated in familial Parkinson’s disease, those encoding LRRK2 and α-synuclein have been associated with neuroinflammatory processes. Previous work showed that LRRK2 is a positive regulator of inflammation, while aggregated α-synuclein released by dying neurons can activate microglia triggering the neuroinflammatory process. The main goal of this project was to gain novel insights into the contribution of microglial cells to the pathogenic mechanisms of LRRK2-associated Parkinson’s

APA, Harvard, Vancouver, ISO, and other styles

47

Baeri, Alberto. "Vers l'élaboration d'un test in vitro pour prédire la nature cancérigène de composés chimiques et naturels utilisés dans l'industrie cosmétique." Electronic Thesis or Diss., Université Côte d'Azur, 2021. http://www.theses.fr/2021COAZ6034.

Full text

Abstract:

Les tests toxicologiques des produits cosmétiques étaient classiquement réalisés sur des modèles animaux. Les coûts prohibitifs et l'évolution de la perception de l'expérimentation animale dans le grand public ont encouragé le développement de tests in vitro capables de prédire la toxicité de composés potentiellement classables "CMR" (Cancérigène, Mutagène et/ou Reprotoxique). Plus récemment, en cosmétique, les tests basés sur l'expérimentation animale ont été interdits dans l'Union européenne.Dans ce travail, nous avons comparé les signatures transcriptomiques de composés cancérigènes non gén

APA, Harvard, Vancouver, ISO, and other styles

48

Campbell, Warren Alexander IV. "Immunomodulatory Signaling Factors that Regulate Müller Glia Reprogramming and Glial Reactivity." The Ohio State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1618173564322887.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Chlis, Nikolaos Kosmas [Verfasser], Fabian J. [Akademischer Betreuer] Theis, Björn [Gutachter] Menze, and Fabian J. [Gutachter] Theis. "From RNA-sequencing to imaging flow cytometry: Profiling large single-cell datasets with machine learning / Nikolaos Kosmas Chlis ; Gutachter: Björn Menze, Fabian J. Theis ; Betreuer: Fabian J. Theis." München : Universitätsbibliothek der TU München, 2020. http://d-nb.info/1221719327/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Li, Qike, A. Grant Schissler, Vincent Gardeux, et al. "N-of-1-pathways MixEnrich: advancing precision medicine via single-subject analysis in discovering dynamic changes of transcriptomes." BIOMED CENTRAL LTD, 2017. http://hdl.handle.net/10150/625841.

Full text

Abstract:

Background: Transcriptome analytic tools are commonly used across patient cohorts to develop drugs and predict clinical outcomes. However, as precision medicine pursues more accurate and individualized treatment decisions, these methods are not designed to address single-patient transcriptome analyses. We previously developed and validated the N-of-1-pathways framework using two methods, Wilcoxon and Mahalanobis Distance (MD), for personal transcriptome analysis derived from a pair of samples of a single patient. Although, both methods uncover concordantly dysregulated pathways, they are not d

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!