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Journal articles on the topic 'Single-Cell Selectivity'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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1

Wertz, Adrian, Stuart Trenholm, Keisuke Yonehara, et al. "Single-cell–initiated monosynaptic tracing reveals layer-specific cortical network modules." Science 349, no. 6243 (2015): 70–74. http://dx.doi.org/10.1126/science.aab1687.

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Individual cortical neurons can selectively respond to specific environmental features, such as visual motion or faces. How this relates to the selectivity of the presynaptic network across cortical layers remains unclear. We used single-cell–initiated, monosynaptically restricted retrograde transsynaptic tracing with rabies viruses expressing GCaMP6s to image, in vivo, the visual motion–evoked activity of individual layer 2/3 pyramidal neurons and their presynaptic networks across layers in mouse primary visual cortex. Neurons within each layer exhibited similar motion direction preferences,
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2

Mao, Zhu, Zhuo Liu, Jin Yang, Xiaoxia Han, Bing Zhao, and Chun Zhao. "In situ semi-quantitative assessment of single-cell viability by resonance Raman spectroscopy." Chemical Communications 54, no. 52 (2018): 7135–38. http://dx.doi.org/10.1039/c8cc01336e.

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We developed a novel method for quantifying single-cell viability with high selectivity by resonance Raman scattering. This powerful tool will allow researchers to study cellular metabolism at the level of a single cell.
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3

Różycki, Bartosz, and Thomas R. Weikl. "Cooperative Stabilization of Close-Contact Zones Leads to Sensitivity and Selectivity in T-Cell Recognition." Cells 10, no. 5 (2021): 1023. http://dx.doi.org/10.3390/cells10051023.

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T cells are sensitive to 1 to 10 foreign-peptide-MHC complexes among a vast majority of self-peptide-MHC complexes, and discriminate selectively between peptide-MHC complexes that differ not much in their binding affinity to T-cell receptors (TCRs). Quantitative models that aim to explain this sensitivity and selectivity largely focus on single TCR/peptide-MHC complexes, but T cell adhesion involves a multitude of different complexes. In this article, we demonstrate in a three-dimensional computational model of T-cell adhesion that the cooperative stabilization of close-contact zones is sensit
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4

Decramer, Thomas, Elsie Premereur, Mats Uytterhoeven, et al. "Single-cell selectivity and functional architecture of human lateral occipital complex." PLOS Biology 17, no. 9 (2019): e3000280. http://dx.doi.org/10.1371/journal.pbio.3000280.

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5

Pasquier, Jennifer, Damien Rioult, Nadine Abu-Kaoud, et al. "P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods." BioMed Research International 2013 (2013): 1–11. http://dx.doi.org/10.1155/2013/676845.

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Background. P-gp expression has been linked to the efflux of chemotherapeutic drugs in human cancers leading to multidrug resistance. Fluorescence techniques have been widely applied to measure the P-gp activity. In this paper, there is a comparison between the advantages of two fluorescence approaches of commonly available and affordable instruments: the microplate reader (MPR) and the flow cytometer to detect the P-gp efflux activity using calcein-AM.Results. The selectivity, sensibility, and reproducibility of the two methods have been defined. Our results showed that the MPR is more powerf
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Sawamura, Hiromasa, Guy A. Orban, and Rufin Vogels. "Selectivity of Neuronal Adaptation Does Not Match Response Selectivity: A Single-Cell Study of the fMRI Adaptation Paradigm." Neuron 49, no. 2 (2006): 307–18. http://dx.doi.org/10.1016/j.neuron.2005.11.028.

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7

Decramer, Thomas, Elsie Premereur, Mats Uytterhoeven, et al. "Correction: Single-cell selectivity and functional architecture of human lateral occipital complex." PLOS Biology 17, no. 12 (2019): e3000588. http://dx.doi.org/10.1371/journal.pbio.3000588.

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8

Li, Jin, Lilin Wang, Pasi A. Jänne, and G. Mike Makrigiorgos. "Coamplification at Lower Denaturation Temperature-PCR Increases Mutation-Detection Selectivity of TaqMan-Based Real-Time PCR." Clinical Chemistry 55, no. 4 (2009): 748–56. http://dx.doi.org/10.1373/clinchem.2008.113381.

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Abstract Background: DNA genotyping with mutation-specific TaqMan® probes (Applied Biosystems) is broadly used in detection of single-nucleotide polymorphisms but is less so for somatic mutations because of its limited selectivity for low-level mutations. We recently described coamplification at lower denaturation temperature-PCR (COLD-PCR), a method that amplifies minority alleles selectively from mixtures of wild-type and mutation-containing sequences during the PCR. We demonstrate that combining COLD-PCR with TaqMan technology provides TaqMan genotyping with the selectivity needed to detect
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9

Carrasco, Carlos J., Antonio Pastor, María del Mar Conejo, et al. "Synthesis, Characterization, and Preliminary In Vitro Anticancer Activity of Zinc Complexes Containing Amino Acid-Derived Imidazolium-Based Dicarboxylate Ligands." International Journal of Molecular Sciences 26, no. 7 (2025): 3202. https://doi.org/10.3390/ijms26073202.

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Coordination polymers containing zinc and imidazolium-based dicarboxylate ligands, [LR]−, were synthesized by reacting zinc acetate with HLR compounds, 1. The resulting complexes were characterized and structurally identified using single-crystal X-ray diffraction, revealing polymeric structures for the complexes [Zn(LR)2]n (R = Gly, 2a; βAla, 2b) and [Zn(LLeu)2(H2O)2]n (2c). In these structures, the [LR]− ligands adopt a bridging monodentate μ-κ1-O1,κ1-O3 coordination mode, resulting in distorted tetrahedral (2a, 2b) or octahedral (2c) geometries around the zinc center. When the synthesis was
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10

Higginson-Scott, Nathan, Kevin L. Otipoby, and Jo Viney. "PT101: A Treg selective agonist IL-2 mutein therapy for autoimmunity." Journal of Immunology 204, no. 1_Supplement (2020): 237.16. http://dx.doi.org/10.4049/jimmunol.204.supp.237.16.

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Abstract Regulatory T cells (Treg) play a critical role in immune homeostasis and are dysfunctional in many autoimmune diseases. Interleukin 2 (IL-2), via the heterotrimeric IL-2 receptor (CD25/CD122/CD132), drives the proliferation and function of Treg. CD25 loss-of-function in mice is associated with Treg deficiency and widespread autoimmunity. Low dose IL-2 expands Tregs and is being evaluated therapeutically, however it has a narrow selectivity window over conventional T cells and Natural Killer cells which is dose limiting. To enhance IL-2 selectivity for activating and expanding Treg, mu
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11

Kim, John J., Elly Sinkala, and Amy E. Herr. "High-selectivity cytology via lab-on-a-disc western blotting of individual cells." Lab on a Chip 17, no. 5 (2017): 855–63. http://dx.doi.org/10.1039/c6lc01333c.

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12

Chen, Qi, Liming Ding, Lihua Wang, Haijun Yang, and Xinhai Yu. "High Proton Selectivity Sulfonated Polyimides Ion Exchange Membranes for Vanadium Flow Batteries." Polymers 10, no. 12 (2018): 1315. http://dx.doi.org/10.3390/polym10121315.

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High proton selectivity is the ultimate aim for the ion exchange membranes (IEMs). In this study, two kinds of sulfonated polyimides (SPI)—non-fluorinated and fluorine-containing polyimide—with about 40% sulfonation degree were synthesized by one-step high temperature polymerization. High proton selectivity IEMs were prepared and applied in vanadium flow batteries (VFB). The chemical structures, physicochemical properties and single cell performance of these membranes were characterized. The results indicate that high molecular weight of SPIs can guarantee the simultaneous achievement of good
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13

Blais, Brian, Leon N. Cooper, and Harel Shouval. "Formation of Direction Selectivity in Natural Scene Environments." Neural Computation 12, no. 5 (2000): 1057–66. http://dx.doi.org/10.1162/089976600300015501.

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Most simple and complex cells in the cat striate cortex are both orientation and direction selective. In this article we use single-cell learning rules to develop both orientation and direction selectivity in a natural scene environment. We show that a simple principal component analysis rule is inadequate for developing direction selectivity, but that the BCM rule as well as similar higher-order rules can. We also demonstrate that the convergence of lagged and nonlagged cells depends on the velocity of motion in the environment, and that strobe rearing disrupts this convergence, resulting in
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14

Madan, Sanna, Sanju Sinha, Tiangen Chang, et al. "Pan-Cancer Analysis of Patient Tumor Single-Cell Transcriptomes Identifies Promising Selective and Safe Chimeric Antigen Receptor Targets in Head and Neck Cancer." Cancers 15, no. 19 (2023): 4885. http://dx.doi.org/10.3390/cancers15194885.

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Chimeric antigen receptor (CAR) T cell therapies have yielded transformative clinical successes for patients with blood tumors, but their full potential remains to be unleashed against solid tumors. One challenge is finding selective targets, which we define intuitively to be cell surface proteins that are expressed widely by cancer cells but minimally by healthy cells in the tumor microenvironment and other normal tissues. Analyzing patient tumor single-cell transcriptomics data, we first defined and quantified selectivity and safety scores of existing CAR targets for indications in which the
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15

Buckley, Benjamin J., Ashna Kumar, Ashraf Aboelela, et al. "Screening of 5- and 6-Substituted Amiloride Libraries Identifies Dual-uPA/NHE1 Active and Single Target-Selective Inhibitors." International Journal of Molecular Sciences 22, no. 6 (2021): 2999. http://dx.doi.org/10.3390/ijms22062999.

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The K+-sparing diuretic amiloride shows off-target anti-cancer effects in multiple rodent models. These effects arise from the inhibition of two distinct cancer targets: the trypsin-like serine protease urokinase-type plasminogen activator (uPA), a cell-surface mediator of matrix degradation and tumor cell invasiveness, and the sodium-hydrogen exchanger isoform-1 (NHE1), a central regulator of transmembrane pH that supports carcinogenic progression. In this study, we co-screened our library of 5- and 6-substituted amilorides against these two targets, aiming to identify single-target selective
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16

Shapiro, M. S., and T. E. DeCoursey. "Selectivity and gating of the type L potassium channel in mouse lymphocytes." Journal of General Physiology 97, no. 6 (1991): 1227–50. http://dx.doi.org/10.1085/jgp.97.6.1227.

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Type l voltage-gated K+ channels in murine lymphocytes were studied under voltage clamp in cell-attached patches and in the whole-cell configuration. The kinetics of activation of whole-cell currents during depolarizing pulses could be fit by a single exponential after an initial delay. Deactivation upon repolarization of both macroscopic and microscopic currents was mono-exponential, except in Rb-Ringer or Cs-Ringer solution in which tail currents often displayed "hooks," wherein the current first increased or remained constant before decaying. In some cells type l currents were contaminated
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17

Gray, M. A., S. Plant, and B. E. Argent. "cAMP-regulated whole cell chloride currents in pancreatic duct cells." American Journal of Physiology-Cell Physiology 264, no. 3 (1993): C591—C602. http://dx.doi.org/10.1152/ajpcell.1993.264.3.c591.

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Using the whole cell configuration of the patch-clamp technique, we have identified an adenosine 3',5'-cyclic monophosphate (cAMP)-regulated chloride conductance in pancreatic duct cells. Basal whole cell currents in single isolated cells were very low (approximately 5 pA/pF) but could be stimulated 17-fold by elevation of intracellular cAMP. The cAMP-activated currents exhibited 1) a high chloride selectivity, 2) a near linear current-voltage relationship, 3) time and voltage independence, 4) block by 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) but not by 4,4'-diisothiocyanostilbene-2,
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18

Naganawa, Shun, and Masahiro Ito. "MotP Subunit is Critical for Ion Selectivity and Evolution of a K+-Coupled Flagellar Motor." Biomolecules 10, no. 5 (2020): 691. http://dx.doi.org/10.3390/biom10050691.

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The bacterial flagellar motor is a sophisticated nanomachine embedded in the cell envelope. The flagellar motor is driven by an electrochemical gradient of cations such as H+, Na+, and K+ through ion channels in stator complexes embedded in the cell membrane. The flagellum is believed to rotate as a result of electrostatic interaction forces between the stator and the rotor. In bacteria of the genus Bacillus and related species, the single transmembrane segment of MotB-type subunit protein (MotB and MotS) is critical for the selection of the H+ and Na+ coupling ions. Here, we constructed and c
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19

Kohmoto, Osami, Tatsuya Shimizu, Kazuro Sugishita, Koh-ichiro Kinugawa, Toshiyuki Takahashi, and Takashi Serizawa. "Selectivity of felodipine for depolarized ventricular myocytes: a study at the single-cell level." European Journal of Pharmacology 319, no. 2-3 (1997): 355–63. http://dx.doi.org/10.1016/s0014-2999(96)00867-9.

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20

Soejima, M., and S. Kokubun. "Single anion-selective channel and its ion selectivity in the vascular smooth muscle cell." Pflügers Archiv European Journal of Physiology 411, no. 3 (1988): 304–11. http://dx.doi.org/10.1007/bf00585119.

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21

Canady, Taylor D., April S. Berlyoung, Joe A. Martinez, et al. "Enhanced Hybridization Selectivity Using Structured GammaPNA Probes." Molecules 25, no. 4 (2020): 970. http://dx.doi.org/10.3390/molecules25040970.

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High affinity nucleic acid analogues such as gammaPNA (γPNA) are capable of invading stable secondary and tertiary structures in DNA and RNA targets but are susceptible to off-target binding to mismatch-containing sequences. We introduced a hairpin secondary structure into a γPNA oligomer to enhance hybridization selectivity compared with a hairpin-free analogue. The hairpin structure features a five base PNA mask that covers the proximal five bases of the γPNA probe, leaving an additional five γPNA bases available as a toehold for target hybridization. Surface plasmon resonance experiments de
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22

Tsai, Meng-Che, Yohannes Ayele Awoke, Wei-Nien Su, and Bing Joe Hwang. "Value-Added Electrolysis Hydrogen Production Via Methanol Oxidation over a Synergetic Pt1-W Dual-Site Catalyst." ECS Meeting Abstracts MA2024-01, no. 35 (2024): 1923. http://dx.doi.org/10.1149/ma2024-01351923mtgabs.

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Electrochemical water splitting is a promising way of generating hydrogen for future sustainable green energy source technology options to replace nonrenewable and environmentally pollutant hydrocarbon energy sources. However, due to its high overpotential in oxygen evolution reaction (OER) and low-value product O2, some candidate anodic reactions with low potential should be proposed to replace OER, such as alcohol oxidation. Unlike fuel cell applications, partial oxidation is preferred to achieve high-value products rather than complete oxidation. For this purpose, we design a synergistic ef
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Liu, Zhengwei, Faming Wang, Xinping Liu, et al. "Cell membrane–camouflaged liposomes for tumor cell–selective glycans engineering and imaging in vivo." Proceedings of the National Academy of Sciences 118, no. 30 (2021): e2022769118. http://dx.doi.org/10.1073/pnas.2022769118.

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The dynamic change of cell-surface glycans is involved in diverse biological and pathological events such as oncogenesis and metastasis. Despite tremendous efforts, it remains a great challenge to selectively distinguish and label glycans of different cancer cells or cancer subtypes. Inspired by biomimetic cell membrane–coating technology, herein, we construct pH-responsive azidosugar liposomes camouflaged with natural cancer-cell membrane for tumor cell–selective glycan engineering. With cancer cell–membrane camouflage, the biomimetic liposomes can prevent protein corona formation and evade p
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Lee, Chae Bok, Kang In Lee, Young Jae Kim, et al. "Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages." Biomedicines 10, no. 6 (2022): 1243. http://dx.doi.org/10.3390/biomedicines10061243.

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Plasma-treated media (PTM) serve as an adjuvant therapy to postoperatively remove residual cancerous lesions. We speculated that PTM could selectively kill cells infected with Mycobacterium tuberculosis (Mtb) and remove postoperative residual tuberculous lesions. We therefore investigated the effects of a medium exposed to a non-thermal plasma jet on the suppression of intracellular Mtb replication, cell death, signaling, and selectivity. We propose that PTM elevates the levels of the detoxifying enzymes, glutathione peroxidase, catalase, and ataxia-telangiectasia mutated serine/threonine kina
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Peters, Matthew F., and Clay W. Scott. "Evaluating Cellular Impedance Assays for Detection of GPCR Pleiotropic Signaling and Functional Selectivity." Journal of Biomolecular Screening 14, no. 3 (2009): 246–55. http://dx.doi.org/10.1177/1087057108330115.

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G-protein—coupled receptors can couple to different signal transduction pathways in different cell types (termed cell-specific signaling) and can activate different signaling pathways depending on the receptor conformation(s) stabilized by the activating ligand (functional selectivity). These concepts offer potential for developing pathway-specific drugs that increase efficacy and reduce side effects. Despite significant interest, functional selectivity has been difficult to exploit in drug discovery, in part due to the burden of multiple assays. Cellular impedance assays use an emerging techn
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Smida, Hassiba, Christine Thobie-Gautier, Mohammed Boujtita, and Estelle Lebegue. "(Digital Presentation) Electrochemical Single Impact Method for Electroactive Bacterial Detection Onto Carbon Ultramicroelectrode." ECS Meeting Abstracts MA2022-02, no. 52 (2022): 1992. http://dx.doi.org/10.1149/ma2022-02521992mtgabs.

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This work aims to design a high sensitivity and selectivity biosensor based on the electrochemistry of single impacts onto ultramicroelectrode (UME) to detect and identify various bacterial strains. The main objective is to establish a unique electrochemical signature for each bacterial cell through the individual impact event signal on the surface of the UME [1, 2]. First, we focus on the detection of well-known electroactive Gram-negative bacteria such as Shewanella oneidensis in order to be able to selectively detect these different single cells. In this case, the strategy currently used is
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Corti, Angelo, Maria Luisa Nolli, and Giovanni Cassani. "Differential Detection of Single-Chain and Two-Chain Urokinase-Type Plasminogen Activator by a New Immunoadsorbent-Amidolytic Assay (IAA)." Thrombosis and Haemostasis 56, no. 03 (1986): 407–10. http://dx.doi.org/10.1055/s-0038-1661692.

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SummaryA new immunoadsorbent-amidolytic assay (IAA) for the specific differential detection of two-chain urokinase-type plasminogen activator (tcu-PA) and its single-chain precursor (scu-PA) in cell culture supernatants has been developed. The assay combines the selectivity of immunoassays with the specificity of enzyme activity assays exploiting both the antigenic and enzymatic properties of the two proteins. tcu-PA and scu-PA are selectively immunoadsorbed on the wells of a microtiterplate coated with the monoclonal antibody 5B4 and tested for enzymatic activity before and after activation b
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28

Harmošová, Michaela, Martin Kello, Michal Goga, et al. "Low-Dimensional Compounds Containing Bioactive Ligands. Part XX: Crystal Structures, Cytotoxic, Antimicrobial Activities and DNA/BSA Binding of Oligonuclear Zinc Complexes with Halogen Derivatives of 8-Hydroxyquinoline." Inorganics 11, no. 2 (2023): 60. http://dx.doi.org/10.3390/inorganics11020060.

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Two tetranuclear [Zn4Cl2(ClQ)6]·2DMF (1) and [Zn4Cl2(ClQ)6(H2O)2]·4DMF (2), as well as three dinuclear [Zn2(ClQ)3(HClQ)3]I3 (3), [Zn2(dClQ)2(H2O)6(SO4)] (4) and [Zn2(dBrQ)2(H2O)6(SO4)] (5), complexes (HClQ = 5-chloro-8-hydroxyquinoline, HdClQ = 5,7-dichloro-8-hydroxyquinoline and HdBrQ = 5,7-dibromo-8-hydroxyquinoline) were prepared as possible anticancer or antimicrobial agents and characterized by IR spectroscopy, elemental analysis and single crystal X-ray structure analysis. The stability of the complexes in solution was verified by NMR spectroscopy. Antiproliferative activity and selectiv
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Woythe, Laura, Pranav Madhikar, Natalia Feiner-Gracia, Cornelis Storm, and Lorenzo Albertazzi. "A Single-Molecule View at Nanoparticle Targeting Selectivity: Correlating Ligand Functionality and Cell Receptor Density." ACS Nano 16, no. 3 (2022): 3785–96. http://dx.doi.org/10.1021/acsnano.1c08277.

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30

Tokatlian, Talar, Grace E. Asuelime, Martin S. Naradikian, et al. "Chimeric Antigen Receptors Directed at Mutant KRAS Exhibit an Inverse Relationship Between Functional Potency and Neoantigen Selectivity." Cancer Research Communications 2, no. 1 (2022): 58–65. http://dx.doi.org/10.1158/2767-9764.crc-21-0165.

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Neoantigens are among the most intriguing potential immuno-oncology targets because, unlike many cancer targets that are expressed on normal tissues, they are by definition restricted to cancer cells. Medicines directed at common neoantigens such as mutant KRAS are especially interesting because they may offer the convenience and cost of an off-the-shelf therapy. However, all common KRAS mutations produce proteins that differ from the wild type at a single amino acid, creating challenges for molecular discrimination. We have undertaken an effort to optimize single-chain variable fragments (scF
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Wisnoskey, Brian J., Mark Estacion, and William P. Schilling. "Maitotoxin-induced cell death cascade in bovine aortic endothelial cells: divalent cation specificity and selectivity." American Journal of Physiology-Cell Physiology 287, no. 2 (2004): C345—C356. http://dx.doi.org/10.1152/ajpcell.00473.2003.

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The maitotoxin (MTX)-induced cell death cascade in bovine aortic endothelial cells (BAECs), a model for Ca2+ overload-induced toxicity, reflects three sequential changes in plasmalemmal permeability. MTX initially activates Ca2+-permeable, nonselective cation channels (CaNSC) and causes a massive increase in cytosolic free Ca2+ concentration ([Ca2+]i). This is followed by the opening of large endogenous cytolytic/oncotic pores (COP) that allow molecules <800 Da to enter the cell. The cells then lyse not by rupture of the plasmalemma but through the activation of a “death” channel that lets
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Honigberg, Lee A., Ashley M. Smith, Jun Chen, Patti Thiemann, and Erik Verner. "Targeting Btk in Lymphoma: PCI-32765 Inhibits Tumor Growth in Mouse Lymphoma Models and a Fluorescent Analog of PCI-32765 Is an Active-Site Probe That Enables Assessment of Btk Inhibition In Vivo." Blood 110, no. 11 (2007): 1592. http://dx.doi.org/10.1182/blood.v110.11.1592.1592.

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Abstract There is increasing evidence indicating that B-cell-receptor (BCR) signaling is required for survival of non-Hodgkin’s lymphoma (NHL) cells. Bruton’s tyrosine kinase (Btk) is required for BCR signaling and mutations that inactivate human Btk cause X-linked-agammaglobulinemia, a B-cell immunodeficiency. Although Btk functions selectively in B cells, the Btk active site is structurally similar to the active site in several Src and Abl kinases and as a result, there have been few highly selective small molecule inhibitors of Btk. We have developed a series of covalent Btk inhibitors that
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Alrhmoun, Saleh, Marina Fisher, Julia Lopatnikova, et al. "Targeting Precision in Cancer Immunotherapy: Naturally-Occurring Antigen-Specific TCR Discovery with Single-Cell Sequencing." Cancers 16, no. 23 (2024): 4020. https://doi.org/10.3390/cancers16234020.

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Background: Adoptive cell therapy is the most promising approach for battling cancer, with T cell receptor-engineered T (TCR-T) cell therapy emerging as the most viable option for treating solid tumors. Current techniques for preparing TCR-T cell therapy provide a limited number of candidates TCRs, missing the comprehensive view of the repertoire, which may hinder the identification of the most effective TCRs. Methods: Dendritic cells were primed with immunogenic peptides of the antigen of interest to expand antigen-specific CD8 T lymphocytes from peripheral blood. Following that, the entire r
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Stupin, D. D. "TiN Nanoporous Electrode covered by Single Cell as Bio-Electronic Sensor of Radiation Hazard." Физика и техника полупроводников 52, no. 5 (2018): 508. http://dx.doi.org/10.21883/ftp.2018.05.45852.41.

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AbstractHere we demonstrate a simple label-free bio-electronic radiation (ultra-violet) hazard detector based on non-destructive electrical impedance spectroscopy technique with a single living cell as a sensing element. Radiation results in wrong cell’s functioning and, as corollary, cell’s membrane distortion. The latter results in impedance shift for the electrode covered by the cell. The additional sensitivity of impedance shift is achieved by the simultaneous usage of the TiN porous electrodes and adaptive filtering for impedance data processing. The main advantage of the proposed sensor
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Mangelinck, Adèle, Elodie Molitor, Ibtissam Marchiq, et al. "The combined use of scRNA-seq and network propagation highlights key features of pan-cancer Tumor-Infiltrating T cells." PLOS ONE 19, no. 12 (2024): e0315980. https://doi.org/10.1371/journal.pone.0315980.

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Improving the selectivity and effectiveness of drugs represents a crucial issue for future therapeutic developments in immuno-oncology. Traditional bulk transcriptomics faces limitations in this context for the early phase of target discovery as resulting gene expression levels represent the average measure from multiple cell populations. Alternatively, single cell RNA sequencing can dive into unique cell populations transcriptome, facilitating the identification of specific targets. Here, we generated Tumor-Infiltrating regulatory T cells (TI-Tregs) and exhausted T cells (Tex) gene signatures
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Shouval, Harel, Nathan Intrator, C. Charles Law, and Leon N. Cooper. "Effect of Binocular Cortical Misalignment on Ocular Dominance and Orientation Selectivity." Neural Computation 8, no. 5 (1996): 1021–40. http://dx.doi.org/10.1162/neco.1996.8.5.1021.

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We model a two-eye visual environment composed of natural images and study its effect on single cell synaptic modification. In particular, we study the effect of binocular cortical misalignment on receptive field formation after eye opening. We show that binocular misalignment affects principal component analysis (PCA) and Bienenstock, Cooper, and Munro (BCM) learning in different ways. For the BCM learning rule this misalignment is sufficient to produce varying degrees of ocular dominance, whereas for PCA learning binocular neurons emerge in every case.
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Madan, Sanna, Sanju Sinha, Alejandro A. Schäffer, and Eytan Ruppin. "Abstract LB062: Identifying novel targets for CAR-T therapies from single cell RNA-sequencing data." Cancer Research 83, no. 8_Supplement (2023): LB062. http://dx.doi.org/10.1158/1538-7445.am2023-lb062.

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Abstract Chimeric antigen receptor T (CAR-T) cell therapies have revolutionized cancer treatment. While CAR-T has yielded tremendous clinical success for patients with liquid tumors, its potential remains to be unleashed against solid tumors. One key challenge is identifying optimal targets for these therapies: cell surface proteins that are expressed highly and uniformly by a tumor’s constituent malignant cells, and minimally so by healthy tissues. Employing a systematic, data-driven analysis, we first charted the landscape of existing CAR-T targets in the clinic, identifying the leading targ
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Fairhall, Adrienne L., C. Andrew Burlingame, Ramesh Narasimhan, Robert A. Harris, Jason L. Puchalla, and Michael J. Berry. "Selectivity for Multiple Stimulus Features in Retinal Ganglion Cells." Journal of Neurophysiology 96, no. 5 (2006): 2724–38. http://dx.doi.org/10.1152/jn.00995.2005.

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Under normal viewing conditions, retinal ganglion cells transmit to the brain an encoded version of the visual world. The retina parcels the visual scene into an array of spatiotemporal features, and each ganglion cell conveys information about a small set of these features. We study the temporal features represented by salamander retinal ganglion cells by stimulating with dynamic spatially uniform flicker and recording responses using a multi-electrode array. While standard reverse correlation methods determine a single stimulus feature—the spike-triggered average—multiple features can be rel
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39

Mishina, Yuji M., Christopher J. Wilson, Linda Bruett, et al. "Multiplex GPCR Assay in Reverse Transfection Cell Microarrays." Journal of Biomolecular Screening 9, no. 3 (2004): 196–207. http://dx.doi.org/10.1177/1087057103261880.

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G protein-coupled receptors (GPCRs) are a superfamily of proteins that include some of the most important drug targets in the pharmaceutical industry. Despite the success of this group of drugs, there remains a need to identify GPCR-targeted drugs with greater selectivity, to develop screening assays for validated targets, and to identify ligands for orphan receptors. To address these challenges, the authors have created a multiplexed GPCR assay that measures greater than 3000 receptor: ligand interactions in a single microplate. The multiplexed assay is generated by combining reverse transfec
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40

Bukauskas, Feliksas F., Angele Bukauskiene, and Vytas K. Verselis. "Conductance and Permeability of the Residual State of Connexin43 Gap Junction Channels." Journal of General Physiology 119, no. 2 (2002): 171–86. http://dx.doi.org/10.1085/jgp.119.2.171.

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We used cell lines expressing wild-type connexin43 and connexin43 fused with the enhanced green fluorescent protein (Cx43-EGFP) to examine conductance and perm-selectivity of the residual state of Cx43 homotypic and Cx43/Cx43-EGFP heterotypic gap junction channels. Each hemichannel in Cx43 cell–cell channel possesses two gates: a fast gate that closes channels to the residual state and a slow gate that fully closes channels; the transjunctional voltage (Vj) closes the fast gate in the hemichannel that is on the relatively negative side. Here, we demonstrate macroscopically and at the single-ch
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41

Long Zhu, Wan, Yoonkyung Park, Il-Seon Park, et al. "Improvement of Bacterial Cell Selectivity of Melittin by a Single Trp Mutation with a Peptoid Residue." Protein & Peptide Letters 13, no. 7 (2006): 719–25. http://dx.doi.org/10.2174/092986606777790575.

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42

Wang, Hong-Zhan, and Richard D. Veenstra. "Monovalent Ion Selectivity Sequences of the Rat Connexin43 Gap Junction Channel." Journal of General Physiology 109, no. 4 (1997): 491–507. http://dx.doi.org/10.1085/jgp.109.4.491.

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The relative permeability sequences of the rat connexin 43 (rCx43) gap junction channel to seven cations and chloride were examined by double whole cell patch clamp recording of single gap junction channel currents in rCx43 transfected neuroblastoma 2A (N2A) cell pairs. The measured maximal single channel slope conductances (γj, in pS) of the junctional current-voltage relationships in 115 mM XCl were RbCl (103) ≥ CsCl (102) > KCl (97) > NaCl (79) ≥ LiCl (78) > TMACl (65) > TEACl (53) and for 115 mM KY were KBr (105) > KCl (97) > Kacetate (77) >
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43

Hantani, Rie, Saya Hanawa, Shohei Oie, Kayo Umetani, Toshihiro Sato, and Yoshiji Hantani. "Identification of a New Inhibitor That Stabilizes Interleukin-2-Inducible T-Cell Kinase in Its Inactive Conformation." SLAS DISCOVERY: Advancing the Science of Drug Discovery 24, no. 8 (2019): 854–62. http://dx.doi.org/10.1177/2472555219857542.

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Interleukin-2-inducible T-cell kinase (ITK) plays an important role in T-cell signaling and is considered a promising drug target. As the ATP binding sites of protein kinases are highly conserved, the design of selective kinase inhibitors remains a challenge. Targeting inactive kinase conformations can address the issue of kinase inhibitor selectivity. It is important for selectivity considerations to identify compounds that stabilize inactive conformations from the primary screen hits. Here we screened a library of 390,000 compounds with an ADP-Glo assay using dephosphorylated ITK. After a su
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44

Mohd, Nasiruddin Hushim, Ab Wahab Norfishah, Syarifah Atifah Tn. Mat Zin Tn., and Ghazali Norlia. "Inter-connected coupled lines resonator topology for bandpass filter application." International Journal of Electrical and Computer Engineering (IJECE) 10, no. 3 (2020): 2523–34. https://doi.org/10.11591/ijece.v10i3.pp2523-2534.

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This paper presents an inter-connected side-shorted coupled-line resonator topology as a base cell. The base cell is built from two single-shorted quarter-wavelength coupled-line sections, connected in series to give a half-wavelength coupled-line that creates a single resonance of bandpass filter response. Higher-order bandpass filter is produced by adding new single-shorted coupled-line sections, cascaded in an inter-connected manner to the base cell. This new topology creates a unique arrangement that caused cross coupling effects between the resonators, resulting to the occurrence of trans
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Du, Xiaofei, Joao L. Carvalho-de-Souza, Cenfu Wei, et al. "Loss-of-function BK channel mutation causes impaired mitochondria and progressive cerebellar ataxia." Proceedings of the National Academy of Sciences 117, no. 11 (2020): 6023–34. http://dx.doi.org/10.1073/pnas.1920008117.

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Despite a growing number of ion channel genes implicated in hereditary ataxia, it remains unclear how ion channel mutations lead to loss-of-function or death of cerebellar neurons. Mutations in the geneKCNMA1, encoding the α-subunit of the BK channel have emerged as responsible for a variety of neurological phenotypes. We describe a mutation (BKG354S) inKCNMA1, in a child with congenital and progressive cerebellar ataxia with cognitive impairment. The mutation in the BK channel selectivity filter dramatically reduced single-channel conductance and ion selectivity. The BKG354Schannel trafficked
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Pirota, Valentina, Erica Salvati, Carla Risoldi, et al. "Core-Extended Naphthalene Diimide Dyads as Light-Up Probes with Targeted Cytotoxicity Toward Tumor Cells." Biomolecules 15, no. 2 (2025): 311. https://doi.org/10.3390/biom15020311.

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Within the framework of rational drug design, this study introduces a novel approach to enhance the specificity of small molecules in targeting cancer cells. This approach starts from the use of dyads merging into a single entity, a naphthalene diimide (NDI) and core-extended NDI (ceNDI), both known as G-quadruplex (G4) ligands and fluorescent probes. The strategy aims to leverage the unique diagnostic strengths of the ceNDI moiety featuring red emission by improving its binding affinity and target selectivity through inclusion in dyads built with different linkers. The newly developed NDI-ceN
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Hess, P., J. B. Lansman, and R. W. Tsien. "Calcium channel selectivity for divalent and monovalent cations. Voltage and concentration dependence of single channel current in ventricular heart cells." Journal of General Physiology 88, no. 3 (1986): 293–319. http://dx.doi.org/10.1085/jgp.88.3.293.

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Single channel and whole cell recordings were used to study ion permeation through Ca channels in isolated ventricular heart cells of guinea pigs. We evaluated the permeability to various divalent and monovalent cations in two ways, by measuring either unitary current amplitude or reversal potential (Erev). According to whole cell measurements of Erev, the relative permeability sequence is Ca2+ greater than Sr2+ greater than Ba2+ for divalent ions; Mg2+ is not measurably permeant. Monovalent ions follow the sequence Li+ greater than Na+ greater than K+ greater than Cs+, and are much less perme
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Mikušević, Vedrana, Marina Schrecker, Natalie Kolesova, Miyer Patiño-Ruiz, Klaus Fendler, and Inga Hänelt. "A channel profile report of the unusual K+ channel KtrB." Journal of General Physiology 151, no. 12 (2019): 1357–68. http://dx.doi.org/10.1085/jgp.201912384.

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KtrAB is a key player in bacterial K+ uptake required for K+ homeostasis and osmoadaptation. The system is unique in structure and function. It consists of the K+-translocating channel subunit KtrB, which forms a dimer in the membrane, and the soluble regulatory subunit KtrA, which attaches to the cytoplasmic side of the dimer as an octameric ring conferring Na+ and ATP dependency to the system. Unlike most K+ channels, KtrB lacks the highly conserved T(X)GYG selectivity filter sequence. Instead, only a single glycine residue is found in each pore loop, which raises the question of how selecti
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49

Vlassakis, Julea, Louise L. Hansen, Ryo Higuchi-Sanabria, et al. "Measuring expression heterogeneity of single-cell cytoskeletal protein complexes." Nature Communications 12, no. 1 (2021). http://dx.doi.org/10.1038/s41467-021-25212-3.

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AbstractMultimeric cytoskeletal protein complexes orchestrate normal cellular function. However, protein-complex distributions in stressed, heterogeneous cell populations remain unknown. Cell staining and proximity-based methods have limited selectivity and/or sensitivity for endogenous multimeric protein-complex quantification from single cells. We introduce micro-arrayed, differential detergent fractionation to simultaneously detect protein complexes in hundreds of individual cells. Fractionation occurs by 60 s size-exclusion electrophoresis with protein complex-stabilizing buffer that minim
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50

Lee, Myeongeun, Jaewoong Lee, Yongha Kim, et al. "Bioelectrosynthesis of Signaling Molecules for Selective Modulation of Cell Signaling." Angewandte Chemie International Edition, July 8, 2025. https://doi.org/10.1002/anie.202508192.

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Bioelectrosynthesis holds great potential for studying and regulating biological systems through the in situ synthesis and delivery of cell signaling molecules with high spatiotemporal precision. Despite recent advancements, precise control over multiple signaling molecules within a single platform remains challenging. Here, we introduce a bioelectrosynthesis approach capable of selectively producing two types of signaling molecules from a single precursor. This system leverages multi‐metal sulfide electrocatalysts inspired by denitrifying enzymes, which generate signaling molecules, nitric ox
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