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Dissertations / Theses on the topic 'Single live cell imaging'

Author: Grafiati

Published: 4 June 2021

Last updated: 12 February 2022

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1

Khorshidi, Mohammad Ali. "Live Single Cell Imaging and Analysis Using Microfluidic Devices." Doctoral thesis, KTH, Proteomik och nanobioteknologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-129278.

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Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. This thesis presents work that combines the use of microfluidic devices, optical microscopy and automated image analysis to design various cell biological assays with single cell resolution including cell proliferation, clonal expansion, cell migration, cell-cell interaction and cell viability tracking. In fact, automated high throughpu

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2

Zhai, Weichao. "Microfluidics and live imaging advances : applications in host/pathogen, immunity and stem cell single cell phenotyping." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277189.

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Live single-cell imaging has emerged as an advanced single-cell study tool for approaching a quantitative understanding of many biological questions in recent years. In previous cell studies using bulk cell measurements, the population averages can miss the information from cell to cell variability and mask the underlying signaling networks and mechanisms. Currently, some single cell analysis methods, including but not limited to, live single-cell imaging experiments that built around a fluorescent imaging setup and microfluidic devices enable the measurement and analysis of cell dynamics and

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3

Linnik, Volha. "Functional analysis of a plant virus replication 'factory' using live cell imaging." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4639.

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Plant viruses have developed a number of strategies that enable them to become obligate intracellular parasites of many agricultural crops. Potato virus X (PVX) belongs to a group of positive-sense, single-stranded plant RNA viruses that replicate on host membranes and form elaborate structures known as viral replication complexes (VRCs) that contain viral RNA (vRNA), proteins and host cellular components. VRCs are the principal sites of viral genome replication, virion assembly and packaging of vRNA for export into neighbouring cells. For many animal viruses, host membrane association is cruc

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4

Panday, Namuna. "Scanning Ion Conductance Microscopy for Single Cell Imaging and Analysis." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3477.

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Most biological experiments are performed on an ensemble of cells under the assumption that all cells are identical. However, recent evidence from single cells studies reveals that this assumption is incorrect. Individual cells within the same generation may differ dramatically, and these differences have important consequences for the health and function of the entire living body. I have used Scanning Ion Conductance Microscopy (SICM) for imaging and analysis of topographical change of single cell membrane, which is difficult to be revealed by optical microscopes. Morphological change in the

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Vig, Dhruv Kumar. "Spanning the Continuum: From Single Cell to Collective Migration." Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/566259.

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A cell's ability to sense and respond to mechanical signals highlights the significance of physical forces in biology; however, to date most biomedical research has focused on genetics and biochemical signaling. We sought to further understand the physical mechanisms that guide the cellular migrations that occur in a number of biological processes, such as tissue development and regeneration, bacterial infections and cancer metastasis. We investigated the migration of single cells and determined whether the biomechanics of these cells could be used to elucidate multi-cellular mechanisms. We fi

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6

Wen, Mary Mei. "New strategies for tagging quantum dots for dynamic cellular imaging." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/52150.

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In recent years, semiconductor quantum dots (QDs) have arisen as a new class of fluorescent probes that possess unique optical and electronic properties well-suited for single-molecule imaging of dynamic live cell processes. Nonetheless, the large size of conventional QD-ligand constructs has precluded their widespread use in single-molecule studies, especially on cell interiors. A typical QD-ligand construct can range upwards of 35 nm in diameter, well exceeding the size threshold for cytosolic diffusion and posing steric hindrance to binding cell receptors. The objective of this research is

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Tavassoly, Iman. "Dynamics of Cell Fate Decisions Mediated by the Interplay of Autophagy and Apoptosis in Cancer Cells: Mathematical Modeling and Experimental Observations." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/79557.

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Autophagy is a conserved biological stress response in mammalian cells that is responsible for clearing damaged proteins and organelles from the cytoplasm and recycling their contents via the lysosomal pathway. In cases where the stress is not too severe, autophagy acts as a survival mechanism. In cases of severe stress, it may lead to programmed cell death. Autophagy is abnormally regulated in a wide-range of diseases, including cancer. To integrate the existing knowledge about this decision process into a rigorous, analytical framework, we built a mathematical model of cell fate decision med

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8

Dursun, Ezgi [Verfasser], Anne [Akademischer Betreuer] Krug, Thomas [Gutachter] Korn, and Markus [Gutachter] Gerhard. "Cell fate decisions of common dendritic cell progenitors characterized by continuous live cell imaging at the single cell level / Ezgi Dursun ; Gutachter: Thomas Korn, Markus Gerhard ; Betreuer: Anne Krug." München : Universitätsbibliothek der TU München, 2015. http://d-nb.info/1114393983/34.

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9

Récamier, Vincent. "Single particle imaging in the cell nucleus : a quantitative approach." Phd thesis, Université René Descartes - Paris V, 2013. http://tel.archives-ouvertes.fr/tel-00998389.

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The cell nucleus is a chemical reactor. Nuclear components interact with each other to express genes, duplicate the chromosomes for cell division, and protect DNA from alteration. These reactions are regulated along the cell cycle and in response to stress. One of the fundamental nuclear processes, transcription, enables the production of a messenger RNA from a template DNA sequence. While mandatory for the cell, transcription nevertheless may involve a very small number of molecules. Indeed, a single gene would have only few copies in the genome. During my PhD, I studied nuclear processes in

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10

Pinaud, Fabien Floren. "Peptide-coated semiconductor quantum dots and their applications in biological imaging of single molecules in live cells and organisms." Diss., Restricted to subscribing institutions, 2007. http://proquest.umi.com/pqdweb?did=1383485011&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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11

Chen, Huiyi. "System-Wide Studies of Gene Expression in Escherichia coli by Fluorescence Microscopy and High Throughput Sequencing." Thesis, Harvard University, 2011. http://dissertations.umi.com/gsas.harvard:10044.

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Gene expression is a fundamental process in the cell and is made up of two parts – the information flow from DNA to RNA, and from RNA to protein. Here, we examined specific sub-processes in Escherichia coli gene expression using newly available tools that permit genome-wide analysis. We begin our studies measuring mRNA and protein abundances in single cells by single-molecule fluorescence microscopy, and then focus our attention to studying RNA generation and degradation by high throughput sequencing. The details of the dynamics of gene expression can be observed from fluctuations in mRNA and

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12

Caccianini, Laura. "Imagerie de l'architecture dynamique de la chromatine dans la cellule unique." Thesis, Paris Sciences et Lettres (ComUE), 2019. https://tel.archives-ouvertes.fr/tel-02896692.

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La structure de la chromatine joue un rôle crucial dans la régulation de plusieurs fonctions cellulaires chez les cellules de mammifères. Perturber l’organisation spatiale de la chromatine peut avoir des conséquences dramatiques sur la vie d’une cellule et peut amener`des pathologies graves chez les organismes. Deux facteurs nucléaires, CTCF et Cohesine, sont parmi les principaux acteurs dans la régulation et le maintien de l’architecture de l’ADN. Des avancements importants ont révélé ́la complexité ́des mécanismes qui régulent l’organisation de la chromatine, mais le domaine manque encore d

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13

Verkhedkar, Ketki Dinesh. "Quantitative Analysis of DNA Repair and p53 in Individual Human Cells." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10660.

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The goal of my research was to obtain a quantitative understanding of the mechanisms of DNA double-strand break (DSB) repair, and the activation of the tumor suppressor p53 in response to DSBs in human cells. In Chapter 2, we investigated how the kinetics of repair, and the balance between the alternate DSB repair pathways, nonhomologous end-joining (NHEJ) and homologous recombination (HR), change with cell cycle progression. We developed fluorescent reporters to quantify DSBs, HR and cell cycle phase in individual, living cells. We show that the rates of DSB repair depend on the cell cycle st

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14

Strawbridge, Stanley Eugene. "Understanding the dynamics of embryonic stem cell differentiation." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/287576.

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The two defining features of mouse embryonic stem (ES) cells are self-renewal and naive pluripotency, the ability to give rise to all cell lineages in the adult body. In addition to being a unique and interesting cell type, pluripotent ES cells have demonstrated their potential for continued advancements in biomedical science. Currently, there is an improved understanding in the chemical signals and the gene regulatory network responsible for the maintenance of ES cells in the naive pluripotent state. However, less is understood about how ES cells exit pluripotency. My main aim is to study the

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15

Conic, Sascha. "Analysis of transcription factor and histone modification dynamics in the nucleus of single living cells using a novel antibody-based imaging approach." Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAJ081.

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Dans les cellules des eucaryotes, la transcription des gènes est contrôlée par une pléthore de complexes protéiniques. Cependant, la plupart de nos connaissances fondamentales sur la régulation de la transcription viennent des expériences biochimiques ou des expériences d’immunofluorescences utilisant des cellules fixées. Par conséquent, beaucoup d’efforts ont été consacré récemment pour obtenir des informations sur les mouvements dynamiques ou sur l’assemblage des facteurs de transcription directement dans des cellules vivantes. Nous avons développé une stratégie de marquage, appelé « versati

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16

Kosmacek, Elizabeth Anne Ianzini Fiorenza Mackey Michael A. "Live cell imaging technology development for cancer research." [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/388.

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17

Büchele, Benjamin. "Live Cell Imaging des Hepatitis C Virus Replikationskomplexes." [S.l. : s.n.], 2004. http://nbn-resolving.de/urn:nbn:de:bsz:25-opus-59102.

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18

Chyan, Wen Ph D. Massachusetts Institute of Technology. "Fluorogenic probes for live-cell imaging of biomolecules." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/118216.

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Thesis: Ph. D. in Biological Chemistry, Massachusetts Institute of Technology, Department of Chemistry, 2018.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 231-249).<br>Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are essential tools for chemical biology. Probes that detect enzymatic activity can be used to illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and sensitivity unmatched by other techniques. This dissertation describes the development

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Kosmacek, Elizabeth Anne. "Live cell imaging technology development for cancer research." Diss., University of Iowa, 2009. https://ir.uiowa.edu/etd/388.

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Live cell imaging is a unique tool for cellular research with a wide variety of applications. By streaming digital microscopic images an investigator can observe the dynamic morphology of a cell, track cell movement on a surface, and measure quantities or localization patterns of fluorescently labeled proteins or molecules. Digital image sequences contain a vast amount of information in the form of visually detectable morphological changes in the cell. We designed computer programs that allow the manual identification of visible events in live cell digital image sequences [Davis et al. 2007].

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20

Di, Paolo Diana. "Single-molecule imaging of electroporated chemotaxis proteins in live bacteria." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:7aa5fabc-2237-480f-8615-5225f009df82.

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Many species of motile bacteria use rotating extracellular filaments to propel themselves through liquid media. Each filament is driven by a membrane spanning rotary nano-machine called the bacterial flagellar motor. In Escherichia coli and Rhodobacter sphaeroides the motor is powered by a transmembrane flux of H+ and the chemical energy is converted into work through a ring of stator units pushing on a central rotor. Chemotaxis is the biasing of movement towards regions that contain higher concentrations of beneficial, or lower concentrations of toxic, chemicals and is one of the most well-un

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21

Sörman, Paulsson Elsa. "Evaluation of In-Silico Labeling for Live Cell Imaging." Thesis, Umeå universitet, Institutionen för fysik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-180590.

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Today new drugs are tested on cell cultures in wells to minimize time, cost, andanimal testing. The cells are studied using microscopy in different ways and fluorescentprobes are used to study finer details than the light microscopy can observe.This is an invasive method, so instead of molecular analysis, imaging can be used.In this project, phase-contrast microscopy images of cells together with fluorescentmicroscopy images were used. We use Machine Learning to predict the fluorescentimages from the light microscopy images using a strategy called In-Silico Labeling.A Convolutional Neural Netw

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22

Han, Hongqing. "Towards accurate and efficient live cell imaging data analysis." Doctoral thesis, Humboldt-Universität zu Berlin, 2021. http://dx.doi.org/10.18452/22324.

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Dynamische zelluläre Prozesse wie Zellzyklus, Signaltransduktion oder Transkription zu analysieren wird Live-cell-imaging mittels Zeitraffermikroskopie verwendet. Um nun aber Zellabstammungsbäume aus einem Zeitraffervideo zu extrahieren, müssen die Zellen segmentiert und verfolgt werden können. Besonders hier, wo lebende Zellen über einen langen Zeitraum betrachtet werden, sind Fehler in der Analyse fatal: Selbst eine extrem niedrige Fehlerrate kann sich amplifizieren, wenn viele Zeitpunkte aufgenommen werden, und damit den gesamten Datensatz unbrauchbar machen. In dieser Arbeit verwende

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23

Flaccavento, Giselle. "Imaging tools for live cell micro-irradiation survival studies." Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589627.

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Micro-irradiation systems are used to analyse the effect of ionizing radiation at the cellular and tissue level, targeting individual cells within a population with a controlled low dose. Cell survival experiments using micro-irradiation systems are limited by factors including: 1) the radiation attenuation and optical properties of the chosen cell dish substrate, 2) the registration of the cell dish before and after irradiation or between multiple imaging modalities and 3) the analysis of the cell or colony growth after irradiation. In this thesis, a set of tools have been developed to improv

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24

Saurabh, Saumya. "Ultra-Photostable Genetically Targeted Fluoromodules for Live Cell Imaging." Research Showcase @ CMU, 2014. http://repository.cmu.edu/dissertations/1020.

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25

Babic, Julien. "New microfluidic systems for controlling the cell microenvironment during live-cell imaging." Thesis, Rennes 1, 2017. http://www.theses.fr/2017REN1B047/document.

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Connaître en temps réel la réponse et le comportement des cellules et organismes modèles suite à des changements de leur environnement, ou à des modulations de leurs fonctions biologiques est devenu essentiel dans les sciences du vivant. Ces réponses nous permettent ensuite de comprendre les mécanismes qui régissent le fonctionnement des cellules vivantes, avec des implications en recherche fondamentale, appliquée et biomédicale. Un des plus gros défis technologiques reste le contrôle des paramètres environnementaux en microscopie haute résolution. De nos jours, aucun système ne permet de régu

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26

Miura, Haruko. "Live-Cell Imaging of Stress Signaling Dynamics in a Cell Fate Decision." Kyoto University, 2019. http://hdl.handle.net/2433/236635.

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27

Relich, Peter Kristopher II. "Single Particle Tracking| Analysis Techniques for Live Cell Nanoscopy." Thesis, The University of New Mexico, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10251887.

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<p> Single molecule experiments are a set of experiments designed specifically to study the properties of individual molecules. It has only been in the last three decades where single molecule experiments have been applied to the life sciences; where they have been successfully implemented in systems biology for probing the behaviors of sub-cellular mechanisms. The advent and growth of super-resolution techniques in single molecule experiments has made the fundamental behaviors of light and the associated nano-probes a necessary concern amongst life scientists wishing to advance the state of h

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28

Sauer, Anna Magdalena. "Live-cell imaging of drug delivery by mesoporous silica nanoparticles." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-138222.

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29

Forsgren, Edvin. "Deep Learning to Enhance Fluorescent Signals in Live Cell Imaging." Thesis, Umeå universitet, Institutionen för fysik, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-175328.

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30

Rosa, Stefanie. "Chromatin dynamics in Arbidopsis development: a live cell imaging approach." Doctoral thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica, 2011. http://hdl.handle.net/10362/6847.

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Dissertation presented to obtain the Ph.D degree in Biology<br>The proper development of multicellular organisms demands the distinct specification of a variety of specialized cell types. While this is one of the oldest statements of developmental genetics, how different patterns of gene expression are established in genetically identical cells and maintained during somatic cell divisions is still an active topic of research. Chromatin structure is now recognized to regulate gene activity playing a crucial role in cell differentiation and development. Chromatin is not simply a packaging

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31

Walling, Maureen A. "Single cell analysis for the characterization of cell populations using a live cell array." STATE UNIVERSITY OF NEW YORK AT ALBANY, 2012. http://pqdtopen.proquest.com/#viewpdf?dispub=3488306.

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32

Agrawal, Amit. "Nanoparticle Probes for Ultrasensitive Biological Detection and Motor Protein Tracking inside Living Cells." Diss., Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/19798.

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Semiconductor quantum dots (QDs) have emerged as a new class of fluorescent probes and labeling agents for biological samples. QDs are bright, highly photostable and allow simultaneous excitation of multiple emissions. Owing to these properties, QDs hold exceptional promise in enabling intracellular biochemical studies and diagnosis with unprecedented sensitivity and accuracy. However, use of QD probes inside living cells remains a challenge due to difficulties in delivery of nanoparticles without causing aggregation and imaging single nanoparticles inside living cells. In this dissertation, a

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33

Yao, Zhizhong. "Using Live Cell Imaging to Probe Biogenesis of the Gram-Negative Cell Envelope." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10230.

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In Gram-negative bacteria, the three-layered cell envelope, including the cell wall, outer and inner membranes, is essential for cell survival in the changing, and often hostile environments. Conserved in all prokaryotes, the cell wall is incredibly thin, yet it functions to prevent osmotic lysis in diluted conditions. Based on observations obtained by genetic and chemical perturbations, time-lapse live cell imaging, quantitative imaging and statistical analysis, Part I of this dissertation explores the molecular and physical events leading to cell lysis induced by division-specific beta-lacta

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34

Smith, David. "Process monitoring and control using live cell imaging for the manufacturing of cell therapies." Thesis, Loughborough University, 2014. https://dspace.lboro.ac.uk/2134/16063.

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Regenerative medicine (RM) represents a promising enabling technology to revolutionize healthcare. This said there are still major gaps between the commercial promise and the reality of the cell therapy sector of regenerative medicine. There is consensus to develop high through-put, automated technologies for the manufacture of RM products. Imaging methods will have the capacity to contribute to this technological gap for cell therapies and are particularly attractive to provide non-destructive monitoring with high spatial and temporal resolution. This work applied an automated, non-invasive p

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35

Hailey, Dale W. "Live cell imaging to study the assembly and fate of autophagosomes." College Park, Md.: University of Maryland, 2008. http://hdl.handle.net/1903/8796.

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Thesis (Ph. D.) -- University of Maryland, College Park, 2008.<br>Thesis research directed by: Dept. of Cell Biology and Molecular Genetics. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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Auciello, Giulio. "Analysis of FGF receptor signalling and trafficking by live-cell imaging." Thesis, University of Birmingham, 2013. http://etheses.bham.ac.uk//id/eprint/4650/.

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Fibroblast growth factor receptors (FGFRs) regulate fundamental cellular processes, including proliferation, differentiation and angiogenesis and have emerged as growth factor receptors central to oncogenesis. This study developed a live-cell assay system for studying FGFR endocytosis and trafficking by employing both confocal and total internal reflection fluorescence (TIRF) microscopy in cells expressing a previously characterised GFP-tagged FGFR2 construct. Data from this work have demonstrated that endocytosis of activated FGFR occurs through clathrin-mediated endocytosis. Interestingly, F

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Han, Hongqing [Verfasser]. "Towards accurate and efficient live cell imaging data analysis / Hongqing Han." Berlin : Humboldt-Universität zu Berlin, 2021. http://d-nb.info/1226153445/34.

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Hung, Yin Pun. "Single Cell Imaging of Metabolism with Fluorescent Biosensors." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10147.

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Cells utilize various signal transduction networks to regulate metabolism. Nevertheless, a quantitative understanding of the relationship between growth factor signaling and metabolic state at the single cell level has been lacking. The signal transduction and metabolic states could vary widely among individual cells. However, such cell-to-cell variation might be masked by the bulk measurements obtained from conventional biochemical methods. To assess the spatiotemporal dynamics of metabolism in individual intact cells, we developed genetically encoded biosensors based on fluorescent proteins.

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Harriman, Oliver Leon Jacobs. "A system-level approach to single-molecule live-cell fluorescence microscopy." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:81425bd2-6bc3-489e-b159-a2590ffffbb1.

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In this work a system-level approach was taken to the single-molecule fluorescence microscopy of living cells. This primarily involved the unification of relevant information within appropriately structured artefacts that were used to inform and enhance experimentation. Initially the diversity of emerging single-molecule techniques was reviewed and presented with a novel article structure to suit the purpose of designing an experiment (Harriman and Leake 2011). Techniques were grouped by the type of information they could access, rather than the standard organisation centred on the techniques

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Dodgson, Lauren. "Dissecting the molecular mechanisms of Drosophila border cell migration using time-lapse live cell imaging." Thesis, University of Liverpool, 2013. http://livrepository.liverpool.ac.uk/16293/.

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Dissection of the cellular dynamics and molecular pathways that drive collective cell migration is necessary to better understand cellular rearrangements that underpin normal development, as well as disease states such as cancer metastasis. Border cell migration in the Drosophila ovary has proven to be a good model of invasive cell migration, because of its genetic tractability, and also because recent advances in culturing egg chambers ex vivo have facilitated live cell imaging in this system. The aim of this thesis was to further develop and implement live cell imaging approaches, and to app

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41

Mickler, Frauke Martina. "Live-cell imaging elucidates cellular interactions of gene nanocarriers for cancer therapy." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-165829.

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42

Li, Mengyao. "P53 dynamics: single-cell imaging data analysis and modeling." HKBU Institutional Repository, 2014. https://repository.hkbu.edu.hk/etd_oa/59.

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The p53 protein plays a central role in controlling the fate of cancer cells. At moderate levels of DNA damage, the concentration of the phosphorylated form of p53 undergoes temporal oscillation with a period of a few hours. In Dr. Shi’s lab, single-cell measurements were carried out using the p53-YFP fusion proteins and time-lapse fluorescence microscopy. We report here a detailed study of the image data. From the time series of the p53 concentration in individual cells, we deduce the amplitude and period of the oscillation. The pulse-to-pulse and cell-to-cell variability of the oscillation i

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Kwok, Sheldon J. J. "Massively multiplexed imaging probes for comprehensive single-cell analysis." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122129.

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Thesis: Ph. D. in Medical Engineering and Medical Physics, Harvard-MIT Program in Health Sciences and Technology, 2019<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages [165]-191).<br>Optical microscopy techniques are widely used to study cellular physiology in their native tissue environments. In particular, the use of fluorescent probes to tag different cell populations, subcellular compartments, specific proteins and nucleotide sequences has enabled examination of cellular phenotypes with increasingly sophisticated detail. Recent efforts to combine physi

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Munasinghe, Jeeva Prasanna. "NMR microscopic imaging of the single cell : Acetabularia mediterranea." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/28290.

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NMR imaging studies performed in the microscopic realm using the cell organelles of the single celled marine green alga, Acetabularia mediterranea, are presented. The study had two main objectives. First, to attain microscopic spatial resolution. Second, to monitor development stages in the reproductive structure, the cap. The images of caps which are flat, oriented in the xy plane, have been obtained as 2-dimensional images. Using a 270 MHz spectrometer and an imaging probe made in this department, a lower resolution of 40-50 µm is reported. The probable causes for the limitation of resoluti

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Ray, Lucille Alexandria. "Live single cell fluorescence microscopy; from antibiotic resistance detection to mitochondrial dysfunction." University of Akron / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=akron1597342775751888.

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46

Moreira, Severina. "Live imaging and genetic studies of inflammatory cell migration in Drosophila melanogaster embryos." Thesis, University of Bristol, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508069.

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47

Mizusawa, Keigo. "Development of Fluorescent Turn-on Self-assembled Nanoprobes for Imaging Specific Proteins under Live Cell Conditions." 京都大学 (Kyoto University), 2013. http://hdl.handle.net/2433/174966.

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Hamilton, Jason S. "Disease Tissue Imaging and Single Cell Analysis with Mass Spectrometry." Thesis, University of North Texas, 2017. https://digital.library.unt.edu/ark:/67531/metadc984137/.

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Cells have been found to have an inherent heterogeneity that has led to an increase in the development of single-cell analysis methods to characterize the extent of heterogeneity that can be found in seemingly identical cells. With an understanding of normal cellular variability, the identification of disease induced cellular changes, known as biomarkers, may become more apparent and readily detectable. Biomarker discovery in single-cells is challenging and needs to focus on molecules that are abundant in cells. Lipids are widely abundant in cells and play active roles in cellular signaling

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49

Knopp, Marcus. "Analysis of spine plasticity in CA1 hippocampal pyramidal neurons employing live cell nanoscopic imaging." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-173975.

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In der Großhirnrinde von Säugetieren beﬁndet sich die Mehrheit erregender Synapsen auf Dornfortsätzen, kleinen dendritischen Ausbuchtungen, die in Größe und Form stark variieren. Die Auslösung aktivitätsabhängiger synaptischer Langzeitplastizität geht mit strukturellen Veränderungen dendritischer Dornen einher. Da das beugungsbegrenzte Auflösungsvermögen konventioneller Lichtmikroskope nicht ausreicht um die Morphologie der Dornen verlässlich zu untersuchen, stellte die Elektronenmikroskopie bisher das wichtigste bildgebende Verfahren zur Erforschung von struktureller Plastizität dar, blieb da

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50

Zhang, Yun. "Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy." [Ames, Iowa : Iowa State University], 2008.

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