Academic literature on the topic 'Single Stranded DNA Binding Proteins (SSBs)'

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Journal articles on the topic "Single Stranded DNA Binding Proteins (SSBs)"

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Kur, Józef, Marcin Olszewski, Anna Długołecka, and Paweł Filipkowski. "Single-stranded DNA-binding proteins (SSBs) -- sources and applications in molecular biology." Acta Biochimica Polonica 52, no. 3 (2005): 569–74. http://dx.doi.org/10.18388/abp.2005_3416.

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Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea and eukarya. The SSBs share a common core ssDNA-binding domain with a conserved OB (oligonucleotide/oligosaccharide binding) fold. This ssDNA-binding domain was presumably present in the common ancestor to all three major branches of life. In recent years, there has been an increasing interest in SSBs because they are useful for molecular biology methods and for analytical purposes. In this review, we concentrate on recent advances in the discovery of new sources
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Guo, Jun-Tao, and Fareeha Malik. "Single-Stranded DNA Binding Proteins and Their Identification Using Machine Learning-Based Approaches." Biomolecules 12, no. 9 (2022): 1187. http://dx.doi.org/10.3390/biom12091187.

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Single-stranded DNA (ssDNA) binding proteins (SSBs) are critical in maintaining genome stability by protecting the transient existence of ssDNA from damage during essential biological processes, such as DNA replication and gene transcription. The single-stranded region of telomeres also requires protection by ssDNA binding proteins from being attacked in case it is wrongly recognized as an anomaly. In addition to their critical roles in genome stability and integrity, it has been demonstrated that ssDNA and SSB–ssDNA interactions play critical roles in transcriptional regulation in all three d
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Tan, Changgeng, Tong Wang, Wenyi Yang, and Lei Deng. "PredPSD: A Gradient Tree Boosting Approach for Single-Stranded and Double-Stranded DNA Binding Protein Prediction." Molecules 25, no. 1 (2019): 98. http://dx.doi.org/10.3390/molecules25010098.

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Interactions between proteins and DNAs play essential roles in many biological processes. DNA binding proteins can be classified into two categories. Double-stranded DNA-binding proteins (DSBs) bind to double-stranded DNA and are involved in a series of cell functions such as gene expression and regulation. Single-stranded DNA-binding proteins (SSBs) are necessary for DNA replication, recombination, and repair and are responsible for binding to the single-stranded DNA. Therefore, the effective classification of DNA-binding proteins is helpful for functional annotations of proteins. In this wor
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Myler, Logan R., Ignacio F. Gallardo, Yi Zhou, et al. "Single-molecule imaging reveals the mechanism of Exo1 regulation by single-stranded DNA binding proteins." Proceedings of the National Academy of Sciences 113, no. 9 (2016): E1170—E1179. http://dx.doi.org/10.1073/pnas.1516674113.

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Exonuclease 1 (Exo1) is a 5′→3′ exonuclease and 5′-flap endonuclease that plays a critical role in multiple eukaryotic DNA repair pathways. Exo1 processing at DNA nicks and double-strand breaks creates long stretches of single-stranded DNA, which are rapidly bound by replication protein A (RPA) and other single-stranded DNA binding proteins (SSBs). Here, we use single-molecule fluorescence imaging and quantitative cell biology approaches to reveal the interplay between Exo1 and SSBs. Both human and yeast Exo1 are processive nucleases on their own. RPA rapidly strips Exo1 from DNA, and this act
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Xu, Longfu, Matthew T. J. Halma, and Gijs J. L. Wuite. "Unravelling How Single-Stranded DNA Binding Protein Coordinates DNA Metabolism Using Single-Molecule Approaches." International Journal of Molecular Sciences 24, no. 3 (2023): 2806. http://dx.doi.org/10.3390/ijms24032806.

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Single-stranded DNA-binding proteins (SSBs) play vital roles in DNA metabolism. Proteins of the SSB family exclusively and transiently bind to ssDNA, preventing the DNA double helix from re-annealing and maintaining genome integrity. In the meantime, they interact and coordinate with various proteins vital for DNA replication, recombination, and repair. Although SSB is essential for DNA metabolism, proteins of the SSB family have been long described as accessory players, primarily due to their unclear dynamics and mechanistic interaction with DNA and its partners. Recently-developed single-mol
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Ruvolo, Peter P., Kathleen M. Keating, Kenneth R. Williams, and John W. Chase. "Single-stranded DNA binding proteins (SSBs) from prokaryotic transmissible plasmids." Proteins: Structure, Function, and Genetics 9, no. 2 (1991): 120–34. http://dx.doi.org/10.1002/prot.340090206.

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Chen, Kuan-Lin, Jen-Hao Cheng, Chih-Yang Lin, Yen-Hua Huang, and Cheng-Yang Huang. "Characterization of single-stranded DNA-binding protein SsbB fromStaphylococcus aureus: SsbB cannot stimulate PriA helicase." RSC Advances 8, no. 50 (2018): 28367–75. http://dx.doi.org/10.1039/c8ra04392b.

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Huang, Yen-Hua, I.-Chen Chen, and Cheng-Yang Huang. "Characterization of an SSB–dT25 complex: structural insights into the S-shaped ssDNA binding conformation." RSC Advances 9, no. 69 (2019): 40388–96. http://dx.doi.org/10.1039/c9ra09406g.

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Dubiel, Katarzyna, Camille Henry, Lisanne M. Spenkelink, et al. "Development of a single-stranded DNA-binding protein fluorescent fusion toolbox." Nucleic Acids Research 48, no. 11 (2020): 6053–67. http://dx.doi.org/10.1093/nar/gkaa320.

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Abstract Bacterial single-stranded DNA-binding proteins (SSBs) bind single-stranded DNA and help to recruit heterologous proteins to their sites of action. SSBs perform these essential functions through a modular structural architecture: the N-terminal domain comprises a DNA binding/tetramerization element whereas the C-terminus forms an intrinsically disordered linker (IDL) capped by a protein-interacting SSB-Ct motif. Here we examine the activities of SSB-IDL fusion proteins in which fluorescent domains are inserted within the IDL of Escherichia coli SSB. The SSB-IDL fusions maintain DNA and
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Luo, Xiao, Uli Schwarz-Linek, Catherine H. Botting, Reinhard Hensel, Bettina Siebers, and Malcolm F. White. "CC1, a Novel Crenarchaeal DNA Binding Protein." Journal of Bacteriology 189, no. 2 (2006): 403–9. http://dx.doi.org/10.1128/jb.01246-06.

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ABSTRACT The genomes of the related crenarchaea Pyrobaculum aerophilum and Thermoproteus tenax lack any obvious gene encoding a single-stranded DNA binding protein (SSB). SSBs are essential for DNA replication, recombination, and repair and are found in all other genomes across the three domains of life. These two archaeal genomes also have only one identifiable gene encoding a chromatin protein (the Alba protein), while most other archaea have at least two different abundant chromatin proteins. We performed a biochemical screen for novel nucleic acid binding proteins present in cell extracts
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Dissertations / Theses on the topic "Single Stranded DNA Binding Proteins (SSBs)"

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Johnson, Vinu. "Structural and Biophysical Studies of Single-Stranded DNA Binding Proteins and dnaB Helicases, Proteins Involved in DNA Replication and Repair." University of Toledo / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1198939056.

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Bain, Amanda Louise. "Investigation of the Physiological Role of Ssb1 using an in-vivo Targeted Mouse Model." Thesis, Griffith University, 2013. http://hdl.handle.net/10072/366937.

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Single-stranded DNA binding proteins (SSBs) are critical for binding, protecting and sequestering single-stranded DNA intermediates during multiple cellular transactions, including DNA replication, repair and transcription. The canonical SSB in eukaryotes, Replication Protein A (RPA), is a heterotrimeric protein essential for numerous cellular processes, including DNA repair by homologous recombination (HR). Recently, Richard et al. (2008) identified a novel human SSB, designated human Single-Stranded DNA Binding protein 1 (hSSB1), critical to DNA repair and the maintenance of genomic stabilit
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Nag, Purba. "Delineating the overlapping roles of the single-stranded DNA binding proteins Ssb1 and Ssb2 in the maintenance of genomic stability and intestinal homeostasis." Thesis, Griffith University, 2019. http://hdl.handle.net/10072/384796.

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Single stranded DNA (ssDNA) binding proteins (SSBPs), are known key players of DNA damage response (DDR) pathway and play an essential role in stabilising fragile ssDNA generated during DNA replication, transcription and repair. The canonical SSBP is the heterotrimeric Replication Protein A (RPA) which is involved in a number of key cellular processes including replication and repair via Homologous Recombination (HR) in the course of DNA damage. Our lab recently described two new SSBPs, termed SSB1 and SSB2 (also known as NABP2/OBFC2B/SOSS-B1 and NABP1/OBFC2A/SOSSB-2, respectively) which form
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Mack, Lynsey A. "Studies of extremophilic single-stranded DNA-binding proteins." Thesis, University of Edinburgh, 2005. http://hdl.handle.net/1842/12512.

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In this study, 6 bacterial SSBs are investigated which have been obtained from 4 different <i>Shewanella</i> strains, from <i>Aquifex aeolicus</i> and from <i>E. coli.</i> The 4 <i>Shewanella</i> SSBs have been taken from strains isolated at different depths of the ocean, from sea-level down to 8600m. These organisms therefore differ in their growth pressure optima. By comparing the characteristics of each of these proteins, differences will lead to clues which relate to the pressure differences. In order to highlight the different adaptations of these proteins, the thermophilic SSB from <i>Aq
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Panjikar, Santosh. "Crystallographic studies of bacterial single stranded DNA-binding proteins." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964154366.

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Jordan, Christian. "Helicase-SSB Interactions In Recombination-Dependent DNA Repair and Replication." ScholarWorks @ UVM, 2014. http://scholarworks.uvm.edu/graddis/270.

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Dda, one of three helicases encoded by bacteriophage T4, has been well- characterized biochemically but its biological role remains unclear. It is thought to be involved in origin-dependent replication, recombination-dependent replication, anti- recombination, recombination repair, as well as in replication fork progression past template-bound nucleosomes and RNA polymerase. One of the proteins that most strongly interacts with Dda, Gp32, is the only single-stranded DNA binding protein (SSB) encoded by T4, is essential for DNA replication, recombination, and repair. Previous studies have shown
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Marton, Richard Francis. "Analysis of the single-stranded DNA binding proteins and associated DNA helicases of Drosophila melanogaster." Thesis, Imperial College London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243868.

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Morten, Michael J. "Developing novel single molecule analyses of the single-stranded DNA binding protein from Sulfolobus solfataricus." Thesis, University of St Andrews, 2015. http://hdl.handle.net/10023/7568.

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Single-stranded DNA binding proteins (SSB) bind to single-stranded DNA (ssDNA) that is generated by molecular machines such as helicases and polymerases. SSBs play crucial roles in DNA translation, replication and repair and their importance is demonstrated by their inclusion across all domains of life. The homotetrameric E. coli SSB and the heterotrimeric human RPA demonstrate how SSBs can vary structurally, but all fulfil their roles by employing oligonucleotide/oligosaccharide binding (OB) folds. Nucleofilaments of SSB proteins bound to ssDNA sequester the ssDNA strands, and in doing so pro
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In, Junghoon Erie Dorothy A. "Structure-function studies of late stages of E. Coli MMR interaction of DNA helicase II with single-stranded DNA binding protein SSB and MutL /." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2008. http://dc.lib.unc.edu/u?/etd,2053.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2008.<br>Title from electronic title page (viewed Feb. 17, 2009). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Curriculum in Applied and Material Sciences." Discipline: Applied and Materials Sciences; Department/School: Applied and Materials Sciences.
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Boutemy, Laurence S. "Analysis of the Interactions between the 5' to 3' Exonuclease and the Single-Stranded DNA-Binding Protein from Bacteriophage T4 and Related Phages." Connect to full text in OhioLINK ETD Center, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1223979052.

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Thesis (Ph. D.)--University of Toledo, 2008.<br>Typescript. "Submitted as partial fulfillment of the requirements for the Doctor of Philosophy in Chemistry." Includes bibliographical references (leaves 305-309).
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Books on the topic "Single Stranded DNA Binding Proteins (SSBs)"

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Oliveira, Marcos T., ed. Single Stranded DNA Binding Proteins. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1290-3.

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Keck, James L., ed. Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8.

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Oliveira, Marcos T. Single Stranded DNA Binding Proteins. Springer, 2022.

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Oliveira, Marcos T. Single Stranded DNA Binding Proteins. Springer, 2021.

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Keck, James L. Single-Stranded DNA Binding Proteins: Methods and Protocols. Humana Press, 2016.

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Choi, Mieyoung. Sequencing and cloning of the N4-coded single-stranded DNA binding protein gene: Identification and functional analysis of active domains. 1992.

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Book chapters on the topic "Single Stranded DNA Binding Proteins (SSBs)"

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Arif, S. M., and M. Vijayan. "Structural Diversity Based on Variability in Quaternary Association. A Case Study Involving Eubacterial and Related SSBs." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_2.

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Vujaklija, Dusica, and Boris Macek. "Detecting Posttranslational Modifications of Bacterial SSB Proteins." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_16.

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Ryzhikov, Mikhail, and Sergey Korolev. "Structural Studies of SSB Interaction with RecO." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_7.

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Zhou, Ruobo, and Taekjip Ha. "Single-Molecule Analysis of SSB Dynamics on Single-Stranded DNA." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_5.

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Kozlov, Alexander G., and Timothy M. Lohman. "SSB Binding to ssDNA Using Isothermal Titration Calorimetry." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_3.

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Page, Asher N., and Nicholas P. George. "Methods for Analysis of SSB–Protein Interactions by SPR." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_12.

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Kozlov, Alexander G., Roberto Galletto, and Timothy M. Lohman. "SSB–DNA Binding Monitored by Fluorescence Intensity and Anisotropy." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_4.

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Marceau, Aimee H. "Ammonium Sulfate Co-precipitation of SSB and Interacting Proteins." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_9.

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Inoue, Jin, and Tsutomu Mikawa. "Use of Native Gels to Measure Protein Binding to SSB." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_13.

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Hedgethorne, Katy, and Martin R. Webb. "Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA." In Single-Stranded DNA Binding Proteins. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_17.

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Conference papers on the topic "Single Stranded DNA Binding Proteins (SSBs)"

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Wang, Wei, and Juan Liu. "Distinguishing single-stranded and double-stranded DNA binding proteins based on structural information." In 2013 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2013. http://dx.doi.org/10.1109/bibm.2013.6732568.

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Casas-Finet, Jose R. "Fluorimetric characterization of tryptophan residues in Escherichia coli single-stranded DNA-binding (SSB) protein and its poly(dT) complex." In OE/LASE '94, edited by Joseph R. Lakowicz. SPIE, 1994. http://dx.doi.org/10.1117/12.182709.

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Reports on the topic "Single Stranded DNA Binding Proteins (SSBs)"

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Elbaum, Michael, and Peter J. Christie. Type IV Secretion System of Agrobacterium tumefaciens: Components and Structures. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7699848.bard.

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Objectives: The overall goal of the project was to build an ultrastructural model of the Agrobacterium tumefaciens type IV secretion system (T4SS) based on electron microscopy, genetics, and immunolocalization of its components. There were four original aims: Aim 1: Define the contributions of contact-dependent and -independent plant signals to formation of novel morphological changes at the A. tumefaciens polar membrane. Aim 2: Genetic basis for morphological changes at the A. tumefaciens polar membrane. Aim 3: Immuno-localization of VirB proteins Aim 4: Structural definition of the substrate
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