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Dissertations / Theses on the topic 'Singola molecola'

Author: Grafiati

Published: 9 March 2023

Last updated: 31 July 2025

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1

CHOUDHARY, DHAWAL. "Studio a livello di singola molecola del folding, misfolding e aggregazione di proteine e dell’attività chaperonica della HSPB8." Doctoral thesis, Università degli studi di Modena e Reggio Emilia, 2020. http://hdl.handle.net/11380/1199862.

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Negli ultimi decenni le pinze ottiche si sono rivelate una tecnica sperimentale estremamente efficace per eseguire studi di spettroscopia di forza a livello di singola molecola. In particolare, un’applicazione delle pinze ottiche che sta avendo una rilevanza biomedica sempre più importante è quella relativa allo studio dei processi di ripiegamento corretto (folding), non corretto (misfolding) e dell’aggregazione di proteine. Di forte rilevanza biomedica è anche la possibilità offerta dalle pinze ottiche di caratterizzare in grande dettaglio i meccanismi molecolari che mediano le interazioni tr

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2

BUGLIONE, ENRICO. "Nanomeccanica per la Ricerca sul Cancro." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2021. http://hdl.handle.net/10281/304787.

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Nonostante gli enormi passi avanti sulla comprensione dei meccanismi d’azione del cancro e nello sviluppo di farmaci antitumorali, la maggior parte delle forme di cancro è ancora incurabile. La ragione di tale insuccesso è radicata nella complessità di questa malattia, che è ancora poco conosciuta sia in fase di insorgenza, dovuta alla misregolazione di oncogeni, sia in fase di metastatizzazione delle cellule cancerose. Lo studio di tali caratteristiche da un punto di vista meccanico può fornire una visione differente dei meccanismi di azione del cancro ed aiutare a contestualizzarli all’inter

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3

CORTI, ROBERTA. "Single molecule force spectroscopy of proteins and DNA." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2020. http://hdl.handle.net/10281/273770.

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Negli ultimi decenni, lo sviluppo di nuove tecniche di singola molecola ha creato le basi per nuovi paradigmi nel campo della biofisica. In particolare, la nanomanipolazione di singole biomacromolecole ha permesso la caratterizzazione meccanica di proteine e DNA, cercando di evidenziare la relazione fondamentale tra struttura e funzione biologica. Nel corso degli anni sono stati sviluppati diversi metodi di nanomanipolazione: tra questi, il Microscopio a Forza Atomica (AFM), il Magnetic Tweezers (MT) e il Flow-Stretching (F-S) accoppiato a fluorescenza. Queste tre tecniche sono state utilizza

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4

Murello, Anna. "La spettroscopia di forza basata sull'AFM nello studio dello spazio conformazionale e dei processi aggregativi di proteine prioniche." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8878/.

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Le malattie neurodegenerative sono caratterizzate da aggregazione proteica, dipendente dalla perdita della usuale struttura fisiologica funzionale delle proteine coinvolte, a favore di conformazioni tossiche (patologiche). Il modello corrente descrive questi cambiamenti conformazionali come eventi rari e ritiene che non esista una sola conformazione patogena, ma che tali possibili conformazioni siano piuttosto eterogenee. La caratterizzazione di queste strutture è, di conseguenza, difficile con le tradizionali tecniche in bulk che permettono di studiare solo la conformazione media e non rendo

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5

Rajagopal, Senthil Arun. "SINGLE MOLECULE ELECTRONICS AND NANOFABRICATION OF MOLECULAR ELECTRONIC DEVICES." Miami University / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=miami1155330219.

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6

Sikor, Martin. "Single-molecule fluorescence studies of Protein Folding and Molecular Chaperones." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-138521.

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7

Ökten, Zeynep. "Single molecule mechanics and the myosin family of molecular motors." [S.l.] : [s.n.], 2006. http://www.diss.fu-berlin.de/2006/6/index.html.

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8

Zhao, Xiaotao. "The synthesis and single-molecule conductance of conjugated molecular wires." Thesis, Durham University, 2014. http://etheses.dur.ac.uk/10634/.

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The past decades have seen the fast development of electronic devices in the industrial sector. There is increasingly rapid growth in the demand for alternative electronic building blocks to compliment, and possibly replace, the conventional silicon-based products. Electronic devices based on organic molecules, especially those based on single molecules, receive intense studies both theoretically and experimentally. In this presented work, a new family of oligo(aryleneethenylene)s (OAE)s with molecular lengths (N…N distance) of ca. 2-6 nm were designed to investigate the length dependence of c

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9

Lange, Jeffrey J. "Studies of molecular motions by fluorescence microscopy at single molecule and single fiber levels." Diss., Manhattan, Kan. : Kansas State University, 2009. http://hdl.handle.net/2097/1629.

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10

Rüttinger, Steffen. "Confocal microscopy and quantitative single molecule techniques for metrology in molecular medicine." [S.l.] : [s.n.], 2006. http://opus.kobv.de/tuberlin/volltexte/2007/1434.

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11

Sikor, Martin [Verfasser]. "Single-molecule fluorescence studies of Protein Folding and Molecular Chaperones / Martin Sikor." München : Verlag Dr. Hut, 2012. http://d-nb.info/1020298650/34.

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12

Iancu, Violeta. "Single Molecule Switches and Molecular Self-Assembly: Low Temperature STM Investigations and Manipulations." Ohio : Ohio University, 2006. http://www.ohiolink.edu/etd/view.cgi?ohiou1159980375.

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13

Schulze, Gunnar [Verfasser]. "Elementary processes in single molecule devices : electronic transport and molecular isomerization / Gunnar Schulze." Berlin : Freie Universität Berlin, 2010. http://d-nb.info/102433547X/34.

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14

Goryaynov, Alexander G. "Molecular Size and Charge Effects on Nucleocytoplasmic Transport Studied By Single-Molecule Microscopy." Bowling Green State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1357278635.

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15

Sapra, K. Tanuj. "Single-Molecule Measurements of Complex Molecular Interactions in Membrane Proteins using Atomic Force Microscopy." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1175696409847-74867.

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Single-molecule force spectroscopy (SMFS) with atomic force microscope (AFM) has advanced our knowledge of the mechanical aspects of biological processes, and helped us take big strides in the hitherto unexplored areas of protein (un)folding. One such virgin land is that of membrane proteins, where the advent of AFM has not only helped to visualize the difficult to crystallize membrane proteins at the single-molecule level, but also given a new perspective in the understanding of the interplay of molecular interactions involved in the construction of these molecules. My PhD work was tightly fo

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16

Vonlanthen, David [Verfasser]. "Biphenyl-Cyclophanes: The Molecular Control over the Conductivity of Single-Molecule Junctions / David Vonlanthen." München : Verlag Dr. Hut, 2010. http://d-nb.info/1009972952/34.

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17

Sapra, K. Tanuj. "Single-Molecule Measurements of Complex Molecular Interactions in Membrane Proteins using Atomic Force Microscopy." Doctoral thesis, Technische Universität Dresden, 2006. https://tud.qucosa.de/id/qucosa%3A24922.

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Single-molecule force spectroscopy (SMFS) with atomic force microscope (AFM) has advanced our knowledge of the mechanical aspects of biological processes, and helped us take big strides in the hitherto unexplored areas of protein (un)folding. One such virgin land is that of membrane proteins, where the advent of AFM has not only helped to visualize the difficult to crystallize membrane proteins at the single-molecule level, but also given a new perspective in the understanding of the interplay of molecular interactions involved in the construction of these molecules. My PhD work was tightly fo

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18

Edman, Lars. "Single molecule dynamics /." Stockholm, 2000. http://diss.kib.ki.se/2000/91-628-4025-8/.

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19

Szumski, Douglas Stewart. "Single molecule spintronics." Thesis, University of Bristol, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.535471.

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20

Davies, Eva Melari. "Single molecule microscopy." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-173355.

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21

Kirstein, Johanna, Christophe Jung, Christian Hellriegel, and Christoph Bräuchle. "Single molecule spectroscopy." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-196553.

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22

Aubin, Sheila Marie Josée. "Single-molecule magnets /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1997. http://wwwlib.umi.com/cr/ucsd/fullcit?p9820882.

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23

Sikor, Martin [Verfasser], and Don [Akademischer Betreuer] Lamb. "Single-molecule fluorescence studies of Protein Folding and Molecular Chaperones / Martin Sikor. Betreuer: Don Lamb." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2011. http://d-nb.info/1019479078/34.

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24

Haque, Md Firoze. "CONTROLLED DEPOSITION OF MAGNETIC MOLECULES AND NANOPARTICLES ON ATOMICALLY FLAT GOLD SURFACES." Master's thesis, University of Central Florida, 2008. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2109.

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In this thesis I am presenting a detailed study to optimize the deposition of magnetic molecules and gold nanoparticles in atomically flat surfaces by self-assembling them from solution. Epitaxially grown and atomically flat gold surface on mica is used as substrate for this study. These surfaces have roughness of the order one tenth of a nanometer and are perfect to image molecules and nanoparticles in the 1-10 nanometers range. The purpose of these studies is to find the suitable parameters and conditions necessary to deposit a monolayer of nano-substance on chips containing gold nanowires w

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25

Lillehei, Peter Thomas. "Single molecule mechanical testing." Diss., Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/31044.

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26

Sedghi, Gita. "Porphyrin single molecular wires." Thesis, University of Liverpool, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.539493.

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27

Pugh, Sara Dorothy. "Single molecule protein folding." Thesis, University of Leeds, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.441320.

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28

Täuber, Daniela, Mario Heidernätsch, Michael Bauer, Günter Radons, Jörg Schuster, and Christian von Borczyskowski. "Single molecule tracking of the molecular mobility in thinning liquid films on thermally grown SiO 2." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-191721.

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Diffusion coefficients obtained from weighted mean square displacements along probe molecule trajectories within ultrathin liquid TEHOS films show a correlation with film thickness. By studying cumulative distributions obtained with a time resolution of 20 ms, we could show that the diffusion is heterogeneous within our liquid films which consist of a few molecular layers only. We detected two components of the diffusion process, a slower and a faster one. Thinning of the film due to evaporation caused a slowdown of the whole diffusion process. But this resulted not from a slowdown in the two

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29

Täuber, Daniela, Mario Heidernätsch, Michael Bauer, Günter Radons, Jörg Schuster, and Christian von Borczyskowski. "Single molecule tracking of the molecular mobility in thinning liquid films on thermally grown SiO 2." Diffusion fundamentals 11 (2009) 107, S. 1-11, 2009. https://ul.qucosa.de/id/qucosa%3A14081.

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Diffusion coefficients obtained from weighted mean square displacements along probe molecule trajectories within ultrathin liquid TEHOS films show a correlation with film thickness. By studying cumulative distributions obtained with a time resolution of 20 ms, we could show that the diffusion is heterogeneous within our liquid films which consist of a few molecular layers only. We detected two components of the diffusion process, a slower and a faster one. Thinning of the film due to evaporation caused a slowdown of the whole diffusion process. But this resulted not from a slowdown in the two

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30

Selvanathan, Pramila. "Photochromic switches for luminescence, plasmonic resonance, single molecule magnetic properties, and molecular wires for nano junctions." Thesis, Rennes 1, 2016. http://www.theses.fr/2016REN1S145.

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Ce travail est consacré à la synthèse et la caractérisation des commutateurs et des fils moléculaires incorporant l'unité et le ruthénium organométalliques fractions photochromiques. La première partie traite de lanthanides complexe Yb combiné avec l'unité et le ruthénium acétylure fractions photochromiques afin de moduler la luminescence avec l'aide de redox et de stimuli lumineux. Dans la deuxième partie explique la combinaison d'unités DTE photochromiques avec des fragments acétylures de ruthénium pour fixer sur la surface de nanoparticules métalliques afin d'affiner leur résonance plasmoni

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31

Smith, Neil Ronald. "USING ELECTRON BEAM LITHOGRAPHY TO MAKE ELECTRODES FOR SINGLE MOLECULE ELECTRONICTS." Miami University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=miami1123213432.

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32

Xu, Kewei. "Single Molecule Studies of Diffusion Dynamics in Polymer Thin Films Near Tg." Thesis, Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/16233.

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For polymers near the glass transition, the dynamics in some regions can be orders of magnitude different compared with the dynamics in other regions only a few nanometers away, so called spatial heterogeneity [1]. In this thesis, single molecule fluorescence microscopy as a powerful tool, was applied to study the spatially heterogeneous dynamics, both orientational and translational, within the polymer matrix near the glass transition temperature. With our total internal reflection fluorescence microscopy (TIRFM) methods, many individual fluorescent dye molecules embedded in the poly (isoprop

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33

Maiuri, Paolo. "Single-cell and real-time analysis of transcription rates from integrated HIV-1 provirus." Doctoral thesis, Scuola Normale Superiore, 2009. http://hdl.handle.net/11384/85935.

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Viral RNA biogenesis is a crucial step in the replication of retroviruses that require both the production of a genomic RNA as well as of translation templates. Cellular and viral factors concur in the biogenesis of RNA at the specific sub-nuclear chromatin site where the reaction takes place. The possibility of tracking viral RNA in living cells gives the unique possibility of measuring the kinetic parameters of RNA biogenesis as well as defining the dynamic recruitment of host and viral factors to the site of replication. In order to study the activation of HIV-1 gene expression from the int

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34

Stephenson, James David. "Structural analysis of the HIV-1 5' untranslated region RNA by single molecule FRET and molecular modelling." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610361.

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35

Snizhko, D. "Photone counting for single molecula acts registration." Thesis, КрНУ, 2018. http://openarchive.nure.ua/handle/document/7522.

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The proposed technic that included electrochemiluminescent amplification of the signal assists to obtain feedback on the interaction individual particles including organic molecules with an electrode. In the work the principal posibility to obtain a responce from a signal on ultramicroelectrodes is shown

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36

Sustarsic, Marko. "In vitro, in silico and in vivo studies of the structure and conformational dynamics of DNA polymerase I." Thesis, University of Oxford, 2016. http://ora.ox.ac.uk/objects/uuid:ea317d58-00f7-4fc4-b71b-866b4becf0f7.

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DNA polymerases are a family of molecular machines involved in high-fidelity DNA replication and repair, of which DNA polymerase I (Pol) is one the best-characterized members. Pol is a strand-displacing polymerase responsible for Okazaki fragment synthesis and base-excision repair in bacteria; it consists of three protein domains, which harbour its 5’-3' polymerase, 3’-5’ exonuclease and 5’ endonuclease activities. In the first part of the thesis, we use a combination of single-molecule Förster resonance energy transfer (smFRET) and rigid-body docking to probe the structure of Pol bound to its

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37

McHugh, Toni. "Single molecule mechanics of Kif15." Thesis, University of Warwick, 2015. http://wrap.warwick.ac.uk/77516/.

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Kinesin-12 is a motor protein that has a role in the processes of mitotic spindle formation and maintenance. The human Kinesin-12, Kif15, has been shown to have some functional redundancy with Eg5, a Kinesin-5 that plays key roles in the formation of the bipolar spindle and is a potential target for anti-cancer drugs. Eg5 is thought to contribute to spindle formation by cross-linking and sliding microtubules, however little is known about the mechanism of Kif15. We have used laser tweezers to investigate the mechanical properties of Kif15 compared to those of kinesin-1. We have found that Kif1

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38

Pánek, Dalibor. "Developments for single molecule studies." Thesis, University of Strathclyde, 2010. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=16933.

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Single molecule fluorescence spectroscopy has attracted considerable attention over the past two decades. Measurement on a single entity provides an opportunity to avoid ensemble averaging which is always present in conventional bulk fluorescence measurements. This makes single molecule spectroscopy particularly interesting for biophysics and biochemistry where heterogeneous systems are often encountered. The general interest of this thesis is in studies of single immobilised molecules carried out at room temperature. One of t.he major issues of single molecule spectroscopy is finding a suitab

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39

Lu, Siran. "Single molecule kinetic isotope effect." Thesis, University of Oxford, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.526483.

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40

Li, Yang. "Single Molecule Spintronics and Friction." Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou151561792063398.

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41

Larsson, Chatarina. "Single-molecule Detection in situ." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-98538.

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42

Yoo, Jae. "Polynuclear manganese single-molecule magnets /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p9988317.

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43

Heß, Volkmar [Verfasser], Daniel [Akademischer Betreuer] Bürgler, and Thomas [Akademischer Betreuer] Michely. "Scanning tunneling microscopy of single-molecule magnets and hybrid-molecular magnets: Two approaches to molecular spintronics / Volkmar Heß. Gutachter: Daniel Bürgler ; Thomas Michely." Köln : Universitäts- und Stadtbibliothek Köln, 2016. http://d-nb.info/1099592941/34.

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44

Dekker, Cees. "The appeal of single-molecule and single-cell studies." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-179451.

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45

Dekker, Cees. "The appeal of single-molecule and single-cell studies." Diffusion fundamentals 20 (2013) 9, S. 1, 2013. https://ul.qucosa.de/id/qucosa%3A13532.

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46

Mishra, Abhudaya. "Mixed-metal molecular complexes single-molecule nanomagnets and bioinorganic models of the water oxidizing complex of photosystem II /." [Gainesville, Fla.] : University of Florida, 2006. http://purl.fcla.edu/fcla/etd/UFE0015608.

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47

Ericsson, Olle. "Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8475.

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48

Chen, Liang. "Single molecule and single particle studies of neuronal axonal transport /." May be available electronically:, 2009. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.

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49

Sendler, Torsten. "Leitwertkontrolle einzelner elektrisch kontaktierter Moleküle." Doctoral thesis, Helmholtz-Zentrum Dresden-Rossendorf, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:d120-qucosa-184190.

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Die molekulare Elektronik setzt sich zum Ziel, passive und aktive Bausteine in integrierten Schaltkreisen auf molekularer Ebene zu realisieren. Dabei ist entscheidend, dass sich der elektrische Leitwert der molekularen Bauelemente hinreichend regulieren lässt. Um zu belegen, dass dies möglich ist, wird in dieser Dissertation die gezielte Leitwertkontrolle einzelner über Nanoelektroden kontaktierter Moleküle nachgewiesen. Die erzielten Ergebnisse ergänzen dabei nahtlos aktuellste Studien. Zum einen werden kontaktierte molekulare Schalter durch Bestrahlung mit Licht einer bestimmten Wellenlänge

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50

Lopez, Benjamin J. 1982. "Experimental realization of a feedback ratchet and a method for single-molecule binding studies." Thesis, University of Oregon, 2010. http://hdl.handle.net/1794/11056.

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xii, 112 p. : ill. (some col.)<br>Biological molecular motors exist in an interesting regime of physics where momentum is unimportant and diffusive motion is large. While only exerting small forces, these motors still manage to achieve directed motion and do work. Brownian motors induce directed motion of diffusive particles and are used as models for biological and artificial molecular motors. A flashing ratchet is a Brownian motor that rectifies thermal fluctuations of diffusive particles through the use of a time-dependent, periodic, and asymmetric potential. It has been predicted that a

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