Relevant bibliographies by topics / Site-Directed Mutagenesi

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Site-Directed Mutagenesi'

Author: Grafiati
Published: 9 March 2023
Last updated: 31 July 2025

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Journal articles on the topic "Site-Directed Mutagenesi"

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Loke, Paxton, and Tiow-Suan Sim. "A Comparison of Three Site-Directed Mutagenesis Kits." Zeitschrift für Naturforschung C 56, no. 9-10 (2001): 810–13. http://dx.doi.org/10.1515/znc-2001-9-1021.

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Abstract In this comparative study, three different mutagenesis kits, namely the MutaGene phagemid in vitro mutagenesis kit (Bio-Rad), the Transformerä Site-Directed mutagenesis kit (Clontech) and the Quik-change site-directed mutagenesis kit (Stratagene) were used for the mutagenesis of IPNS genes. However, a large difference in mutation efficiencies among these kits was encountered. Furthermore, these kits employ different strategies with its own individual strengths and weaknesses. Thus, a comparison among these three kits to evaluate their usefulness and improvements on the strategy adopte
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Sil, Kallol, and Soumendra Nath Talapatra. "Prediction of Site Directed Mutagenesis of Acetylcholinesterase by Using Hotspot Wizard Tool." International Journal of Science and Research (IJSR) 13, no. 9 (2024): 1597–600. http://dx.doi.org/10.21275/sr24926121958.

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Carter, P. "Site-directed mutagenesis." Biochemical Journal 237, no. 1 (1986): 1–7. http://dx.doi.org/10.1042/bj2370001.

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de Vries, G. E. "Site-directed mutagenesis." Trends in Plant Science 5, no. 7 (2000): 276. http://dx.doi.org/10.1016/s1360-1385(00)01699-x.

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Nybo, Kristie. "Site-directed mutagenesis: colony growth." BioTechniques 50, no. 2 (2011): 87–89. http://dx.doi.org/10.2144/000113609.

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Erb, Laurie, Richard Garrad, Yanjun Wang, Tom Quinn, John T. Turner, and Gary A. Weisman. "Site-directed Mutagenesis of P2UPurinoceptors." Journal of Biological Chemistry 270, no. 9 (1995): 4185–88. http://dx.doi.org/10.1074/jbc.270.9.4185.

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Weiner, M. P., and G. L. Costa. "Rapid PCR site-directed mutagenesis." Genome Research 4, no. 3 (1994): S131—S136. http://dx.doi.org/10.1101/gr.4.3.s131.

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MacDonald, Nicholas J., José M. P. Freije, Mary L. Stracke, Richard E. Manrow, and Patricia S. Steeg. "Site-directed Mutagenesis ofnm23-H1." Journal of Biological Chemistry 271, no. 41 (1996): 25107–16. http://dx.doi.org/10.1074/jbc.271.41.25107.

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Carey, Michael F., Craig L. Peterson, and Stephen T. Smale. "PCR-Mediated Site-Directed Mutagenesis." Cold Spring Harbor Protocols 2013, no. 8 (2013): pdb.prot076505. http://dx.doi.org/10.1101/pdb.prot076505.

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Costa, Gina L., and Michael P. Weiner. "Rapid PCR Site-Directed Mutagenesis." Cold Spring Harbor Protocols 2006, no. 1 (2006): pdb.prot4144. http://dx.doi.org/10.1101/pdb.prot4144.

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Dissertations / Theses on the topic "Site-Directed Mutagenesi"

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Rúnarsdóttir, Saga. "Site-Directed Mutagenesis Studies of FucO." Thesis, Uppsala universitet, Institutionen för kemi - BMC, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235136.

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Al-Khatib, Haifa Yousef. "Site Directed Mutagenesis of Dienelactone Hydrolase." Thesis, University of North Texas, 1995. https://digital.library.unt.edu/ark:/67531/metadc277940/.

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The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.
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Chen, Wei 1965. "Site Directed Mutagenesis Of Dienelactone Hydrolase." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500900/.

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The role of individual amino acid residues of the enzyme dienelactone hydrolase was investigated. Using the polymerase chain reaction (PCR), a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHI to EcoRI clcD subclone in the plasmid pUC19. Site-specific mutants of dienelactone hydrolase were created using mismatched oligonucleotides to prime DNA synthesis. Specifically modified proteins from mutated clcD genes (Arg 81 to alanine, Tyr 85 to phenylalanine and Arg 206 to alanine), were encoded by the mutant clones. Enzyme assays showed that dienelactone hydrolase activity of
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Marc, Daniel. "La myristylation de la proteine de capside vp4 du poliovirus; son role dans le cycle viral." Paris 7, 1991. http://www.theses.fr/1991PA077059.

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La proteine de capside vp4 du poliovirus, ainsi que des precurseurs vp0 et p1, est myristlee: sa glycine n-terminale est liee de maniere covalente a un acide gras tetra-decanoique. Cette modification co-traductionnelle est determinee par la sequence n-terminale de la proteine (gly#1ala#2gln#3val#4ser#5ser#6). Par mutagenese dirigee a l'aide d'oligonucleotides, nous avons modifie dans le cadn viral la sequence codant pour le signal de myristylation de la proteine. Des transcrits genomiques portant les differentes mutations ont ete synthetises in vitro, et leurs proprietes analysees in vitro par
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White, Malcolm F. "Yeast phosphoglycerate mutase studied by site-directed mutagenesis." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/24419.

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Wang, Xiaoshan. "Site-Directed Mutagenesis in Francisella Tularensis by Allelic." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/36440.

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<p> Francisella tularensis is a Gram-negative, facultative intracellular coccobacillus and the etiologic agent of tularemia for a wide variety of vertebrate and invertebrate animal species. Several species and subspecies of Francisella are currently recognized. However, the majority of infections are caused by F. tularensis subspecies tularensis (type A) and subspecies holarctica (type B). Given the low infectious dose, multiple transmission routes, severity of illness, and lack of licensed vaccines, F. tularensis has long been considered a potential biological weapon and is now classified as
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Bowker-Kinley, Melissa M. "Pyruvate dehydrogenase kinase Kinetics, site-directed mutagenesis, and regulation /." [Bloomington, Ind.] : Indiana University, 2005. http://wwwlib.umi.com/dissertations/fullcit/3183930.

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Thesis (Ph.D.)--Indiana University, 2005.<br>Source: Dissertation Abstracts International, Volume: 66-07, Section: B, page: 3690. Chair: Robert A. Harris. Title from dissertation home page (viewed Oct. 5, 2006).
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Tito, Donald. "Site-directed mutagenesis of hydrogenase genes in Azotobacter chroococcum." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56889.

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Accessory hydrogen uptake genes have been identified in a region of the Azotobacter chroococcum genome about 5 kb downstream of the hydrogenase structural genes (hupSL). DNA sequencing has revealed six genes (hupABYCDE) in this region. These genes are probably transcribed in the same direction as hupSL but are probably in a different operon. Mutational analysis had shown that disruption of the hupB, hupY, hupD and hupE genes gives a Hup$ sp-$ phenotype. In the present work additional mutational analysis, using Tn5, a Tn5 -derivative containing a promoterless lacZ gene, and a kanamycin resistan
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Owegi, Margaret. "Site-directed mutagenesis of yeast V-ATPase subunit d." Virtual Press, 2005. http://liblink.bsu.edu/uhtbin/catkey/1319550.

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V-ATPases are enzymes found in all eukaryotic cells. They are organized into a peripheral membrane complex (V1) and an integral membrane complex (V0). VI is responsible for ATP hydrolysis and generates the energy used by Vo to pump protons from the cytosol into the vacuole. Subunit d is a component of Vo possibly located at the interface between V 1 and V. in the V-ATPase complex. We hypothesize that subunit d could be involved in the structural and functional coupling of VI and Vo. This was tested by generating point mutations along the open reading frame of subunit d from yeast. The mutation
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Schmidt, William Richard. "Site-directed mutagenesis of the ncd microtubule motor protein." Thesis, This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-12302008-063348/.

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Books on the topic "Site-Directed Mutagenesi"

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J, McPherson M., ed. Directed mutagenesis: A practical approach. IRL Press, 1991.

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McPherson, M.J., (Ed.), ed. Directed Mutagenesis: A Practical Approach. I.R.L. P., 1991.

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International Symposium on Site-Directed Mutagenesis and Protein Engineering (1990 Tromsø, Norway). Site-directed mutagenesis and protein engineering: Proceedings of the International Symposium on Site-Directed Mutagensis and Protein Engineering, Tromsø, 27-30 August 1990. Edited by el-Gewely M. Rafaat. Elsevier Science Publishers., 1991.

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International Symposium on Site-Directed Mutagenesis and Protein Engineering (1990 Tromsø, Norway). Site-directed mutagenesis and protein engineering: Proceedings of the International Symposium on Site-Directed Mutagenesis and Protein Engineering, Tromsø, 27-30 August 1990. Edited by El-Gewely M. Rafaat. Elsevier Science Publishers, 1991.

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K, Trower Michael, ed. In vitro mutagenesis protocols. Humana Press, 1996.

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Lloyd, John S. Heterologous expression and site-directed mutagenesis of soluable methane monooxygenase. typescript, 1997.

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Kask, Kalev. Galanin receptor: Studies with site-directed mutagenesis, agonists and antagonists. [s.n.], 1996.

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Provart, Nicholas J. A structure-function analysis of pea carbonic anhydrase by site directed mutagenesis. National Library of Canada = Bibliothèque nationale du Canada, 1993.

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Horst, Bluethmann, and Ohashi Pamela S, eds. Transgenesis and targeted mutagenesis in immunology. Academic Press, 1994.

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Yang, Winnie Yongchen. Site-directed mutagenesis and purification of gpNu1, the small subunit of bacteriophage lambda terminase. National Library of Canada, 1993.

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Book chapters on the topic "Site-Directed Mutagenesi"

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Carrigan, Patricia E., Petek Ballar, and Sukru Tuzmen. "Site-Directed Mutagenesis." In Methods in Molecular Biology. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-61737-954-3_8.

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Adair, John R., and T. Paul Wallace. "Site-Directed Mutagenesis." In Springer Protocols Handbooks. Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-642-3_28.

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James, Roberta M., and Paul Dickinson. "Site-Directed Mutagenesis." In Springer Protocols Handbooks. Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-642-3_29.

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Bhattacharya, Animesh. "Site-directed Mutagenesis." In Encyclopedia of Systems Biology. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_1474.

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Gooch, Jan W. "Site-Directed Mutagenesis." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14811.

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Johnson, Alfred C., and Marvin Reitz. "Site-Directed Mutagenesis." In Recombinant DNA Principles and Methodologies. CRC Press, 2021. http://dx.doi.org/10.1201/9781003067658-19.

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DeSantis, Grace, and J. Bryan Jones. "Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis." In In Vitro Mutagenesis Protocols. Humana Press, 2002. http://dx.doi.org/10.1385/1-59259-194-9:055.

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Strain-Damerell, Claire, and Nicola A. Burgess-Brown. "High-Throughput Site-Directed Mutagenesis." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9624-7_13.

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Chatellier, Jean, and Thierry Vernet. "Combinatorial Scanning Site-Directed Mutagenesis." In Gene Cloning and Analysis. Garland Science, 2023. http://dx.doi.org/10.1201/9781003421474-8.

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McClellan, Michael J. "In Vitro Site Directed Mutagenesis." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3004-4_8.

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Conference papers on the topic "Site-Directed Mutagenesi"

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Holmes, W. E., H. R. Lijnen та D. Collen. "CHARACTERIZATION OFα2-ANTIPLASMIN.REACTIVE SITE VARIANTS PRODUCED BY SITE-DIRECTED MUTAGENESIS". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644766.

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α2-Antiplasmin (α2AP) is the primary physiological plasmin inhibitor in human plasma. The inhibition is rapid (second order rate constants (k1) are expressed as M−1 s−1 ) (k1 = 2 × 107) and occurs as the consequence of an irreversible 1:1 stoichiometric complex formation; the exact nature of and the forces involved in complex formation are not fully understood. In fact, what makes α2AP an inhibitor, rather than simply a substrate remains unresolved. Recently, we deduced the primary structure of α2 AP from the sequence of its cDNA. 95%of this sequence was confirmed by amino acid (aa) sequence a
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Haigwood, N., E.-P. Pâques, G. Mullenbach, G. Moore, L. DesJardin, and A. Tabrizi. "IMPROVEMENT OF T-PA PROPERTIES BY MEANS OF SITE DIRECTED MUTAGENESIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643841.

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The clinical relevance of tissue-plasminogen-activator (t-PA) as a potent thrombolytic agent has recently been established. It has however been recognized that t-PA does not fulfill all conditions required for an ideal thrombolytic pharmaceutical agent; for example, its physiological stability and its short half life in vivo necessitate the use of very large clinical doses. We have therefore attempted to develop novel mutant t-PA proteins with improved properties by creating mutants by site-directed mutagenesis in M13 bacteriophage. Seventeen mutants were designed, cloned, and expressed in CHO
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ISPAS, GABRIELA, I. FAMELAER, FATIMA ELMALKI, and M. JACOBS. "LUCIFERASE KNOCK – OUT MUTANTS BY SITE DIRECTED MUTAGENESIS OF THE AMP BINDING SITE." In Proceedings of the 11th International Symposium. WORLD SCIENTIFIC, 2001. http://dx.doi.org/10.1142/9789812811158_0042.

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Yu, Yao-Chuan. "Site-directed mutagenesis studies support postulated MtNPF1.7 structure and transport mechanism." In ASPB PLANT BIOLOGY 2020. ASPB, 2020. http://dx.doi.org/10.46678/pb.20.1332481.

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Li, Mingtao, Xiaoyu You, and Kunrong Mei. "Site-directed mutagenesis of Saccharomyces cerevisiae genome using mismatch PCR product." In International Conference on Biomedical and Intelligent Systems (IC-BIS 2022), edited by Ahmed El-Hashash. SPIE, 2022. http://dx.doi.org/10.1117/12.2660375.

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Pittman, Debra D., Louise C. Wasley, Beth L. Murray, Jack H. Wang, and Randal J. Kaufman. "ANALYSIS OF STRUCTURAL REQUIREMENTS FOR FACTOR VIII FUNCTION USING SITE-DIRECTED MUTAGENESIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644044.

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Factor VIII (fVIII) functions in the intrinsic pathway of coagulation as the cofactor for Factor IXa proteolytic activation of Factor X. fVIII contains multiple sites which are susceptible to cleavage by thrombin, Factor Xa, and activate) protein C. Proteolytic cleavage is required for cofactor activity and may be responsible for inactivation of cofactor activity. In order to identify the role ofthe individual cleavages of fVIII in its activation and inactivation, site-directed DNA mediated mutagenesis of fVIII was performed and the altered forms of fVIII produced and characterized. Conversion
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Wiedenmann, Joerg, Beatrice Vallone, Fabrizio Renzi, et al. "Dimeric variants of the red fluorescent protein eqFP611 generated by site-directed mutagenesis." In Biomedical Optics 2004, edited by Alexander P. Savitsky, Lubov Y. Brovko, Darryl J. Bornhop, Ramesh Raghavachari, and Samuel I. Achilefu. SPIE, 2004. http://dx.doi.org/10.1117/12.529370.

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Nur, S. A., M. D. Larionova, L. P. Burakova, and E. V. Eremeeva. "THE THERMOSTABILITY ENHANCEMENT OF RENILLA MUELLERI RECOMBINANT LUCIFERASE." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-198.

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The use of luciferase as bioluminescent markers is often complicated by a significant decrease in enzymatic activity at physiological temperatures. To increase the thermal stability of RmY luciferase, a number of amino acid substitutions were introduced into the corresponding genetic construct by site-directed mutagenesis. The thermal stability of RmY luciferase was shown to increase in response to the introduction of selected amino acid substitutions.
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Ren, Qiang, Fei Luo, Weichao Ding, and Haifeng Lu. "An Improved NSGAII Algorithm Based on Site-Directed Mutagenesis Method for Multi-Objective Optimization." In 2019 IEEE Symposium Series on Computational Intelligence (SSCI). IEEE, 2019. http://dx.doi.org/10.1109/ssci44817.2019.9002847.

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He, Yi-Wu, Mark P. Krebs, Judy Herzfeld, H. G. Khorana, and Kenneth J. Rothschild. "FTIR spectroscopy, site-directed mutagenesis, and isotope labeling: a new approach for studying membrane proteins." In Luebeck - DL tentative, edited by Herbert M. Heise, Ernst H. Korte, and Heinz W. Siesler. SPIE, 1992. http://dx.doi.org/10.1117/12.56283.

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Reports on the topic "Site-Directed Mutagenesi"

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Needleman, R. Site-directed mutagenesis of an energy transducing protein: Bacteriorhodopsin. Final report, July 15, 1992--July 14, 1996. Office of Scientific and Technical Information (OSTI), 1998. http://dx.doi.org/10.2172/661520.

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โขวิฑูรกิจ, วีรพันธุ์, วาณี เปล่งพาณิชย์, ปาล์ม ชาติยิ่งเจริญ та LeGoff, Wilfried. โครงการวิจัยการศึกษารหัสพันธุกรรมในคนไทยที่มีไขมันในเลือดชนิดเอชดีแอลสูงมาก โดยวิธีถอดรหัสและวิเคราะห์การเปลี่ยนแปลงหน้าที่. จุฬาลงกรณ์มหาวิทยาลัย, 2011. https://doi.org/10.58837/chula.res.2011.33.

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วัตถุประสงค์: ปัจจัยทางพันธุกรรมที่เกี่ยวข้องกับภาวะเอชดีแอลในเลือดสูงยังไม่เป็นที่เข้าใจแน่ชัด คณะผู้วิจัยทำการถอดรหัสพันธุกรรมยีน 3 ยีน คือ CETP, LIPC และ LIPG ซึ่งสร้างโปรตีน คอเลสเตอริล เอสเทอร์ ทรานสเฟอร์ โปรตีน, เฮบพาติค ไลเปส และ เอนทีเลียล ไลเปส ตามลำดับ ในคนไทยที่มีระดับเอชดีแอลในเลือดสูงมากเทียบกับประชากรกลุ่มควบคุมวิธีดำเนินการวิจัย คณะผู้วิจัยทำการถอดรหัสยีน CETP, LIPC และ LIPG ในส่วนของ exon และ exon-intron junctions เพื่อค้นหาการเปลี่ยนแปลงพันธุกรรมในคนไทย 64 คนที่มีระดับเอชดีแอล ≥ 2.59 มิลลิโมล/ลิตร (100 มิลลิกรัม/เดซิเมตร) และเปรียบเทียบกับผลในประชากรกลุ่มควบคุม 113 คนผลการวิจั
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Gurevitz, Michael, Michael Adams, and Eliahu Zlotkin. Insect Specific Alpha Neurotoxins from Scorpion Venoms: Mode of Action and Structure-Function Relationships. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7613029.bard.

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This study was motivated by the need to develop new means and approaches to the design of future, environmentally-safe, insecticides. Utilization of anti-insect selective toxins from scorpion venoms and clarification of the molecular basis for their specificity, are a major focus in this project and may have an applicative value. Our study concentrated on the highly insecticidal toxin, LqhaIT, and was devoted to: (I) Characterization of the neuropharmacological and electrophysiological features of this toxin. (II) Establishment of a genetic system for studying structure/activity relationships
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Stern, David B., and Gadi Schuster. Manipulation of Gene Expression in the Chloroplast: Control of mRNA Stability and Transcription Termination. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7568750.bard.

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Chloroplasts are the site of photosynthesis and of other essential biosynthetic activities in plant cells. Chloroplasts are semi-autonomous organelles, since they contain their own genomes and protein biosynthetic machinery, but depend on the coordinate expression of nuclear genes to assemble macromolecular complexes. The bioeingineering of plants requires manipulation of chloroplast gene expression, and thus a knowledge of the molecular mechanisms that modulate mRNA and protein production. In this proposal the heterotrophic green alga Chlamydomonas reinhardtii has been used as a model system
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Spiers, Donald, Arieh Gertler, Harold Johnson, and James Spain. An In Vitro and In Vivo Investigation of the Diverse Biological Activities of Bovine Placental Lactogen. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7568087.bard.

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In order to understand the structure-function relationship of bovine placental lactogen (bPL) and initiate production of material for in vivo testing, 28 different bPL analogues were prepared by either truncation or site-directed mutagenesis. The effect of these mutations was determined by measuring binding capacity, ability to homodimerize extracellular domains (ECDs) of several lactogenic and somatogenic receptors, and by in vitro bioassays. Two analogues were prepared in large amounts for in vivo studies. These studies (a) identified the residues responsible for the somatogenic activity of
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Gutnick, David, and David L. Coplin. Role of Exopolysaccharides in the Survival and Pathogenesis of the Fire Blight Bacterium, Erwinia amylovora. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568788.bard.

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Fireblight, a disease of apples and pears, is caused by Erwinia amylovora. Mutants of E. amylovora that do not produce the extreacellular polysaccharide (EPS), amylovoran, are avirulent. A similar EPS, stewartan, is produced by E. stewartii, which caused Stewart's wilt of corn, and which has also been implicated in the virulence of this strain. Both stewartan and amylovoran are type 1 capsular polysaccharides, typified by the colanic acid slime produced by Escherichia coli. Extracellular polysaccharide slime and capsules are important for the virulence of bacterial pathogens of plants and anim
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Stern, David, and Gadi Schuster. Manipulation of Gene Expression in the Chloroplast. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575289.bard.

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The steady-state level of a given mRNA is determined by its rates of transcription and degradation. The stabilities of chloroplast mRNAs vary during plant development, in part regulating gene expression. Furthermore, the fitness of the organelle depends on its ability to destroy non-functional transcripts. In addition, there is a resurgent interest by the biotechnology community in chloroplast transformation due to the public concerns over pollen transmission of introduced traits or foreign proteins. Therefore, studies into basic gene expression mechanisms in the chloroplast will open the door
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Nelson, Nathan, and Charles F. Yocum. Structure, Function and Utilization of Plant Photosynthetic Reaction Centers. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7699846.bard.

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Light capturing and energy conversion by PSI is one of the most fundamental processes in nature. In the heart of these adaptations stand PSI, PSII and their light harvesting antenna complexes. The main goal of this grant proposal was to obtain by X-ray crystallography information on the structure of plant photosystem I (PSI) and photosystem II (PSII) supercomplexes. We achieved several milestones along this line but as yet, like several strong laboratories around the world, we have no crystal structure of plant PSII. We have redesigned the purification and crystallization procedures and recent
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Shai, Yechiel, Arthur Aronson, Aviah Zilberstein, and Baruch Sneh. Study of the Basis for Toxicity and Specificity of Bacillus thuringiensis d-Endotoxins. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7573995.bard.

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The report contains three parts which summarizes the three years achievements of the three participating research groups; The Weizmann group, Tel-Aviv group and Purdue group. The firs part describes the achievements obtained by Shai's group toward the elucidation of the mechanism of membrane insertion and the structural organization of the pores formed by the Cry3A and Cry1Ac B. thuringiensis d-endotoxins. For that purpose Shai's group synthesized, fluorescently labeled and structurally and functionally characterized peptides corresponding to the seven helices that compose the pore-forming dom
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Xu, Jin-Rong, and Amir Sharon. Comparative studies of fungal pathogeneses in two hemibiotrophs: Magnaporthe grisea and Colletotrichum gloeosporioides. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7695585.bard.

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Plant pathogenic fungi have various life styles and different plant infection strategies. Hemibiotrophs like Magnaporthe grisea and Colletotrichum species develop specialized structures during plant infection. The goal of this study was to identify, characterize, and compare genes required for plant infection in M. grisea and C. gloeosporioides. Specific objectives are to: 1) further characterize genes identified in the preliminary studies of C. gloeosporioides and M. grisea;2) identify and characterize additional fungal genes tagged by GFP; and 3) identify in planta growth and appressorium-sp
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