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Dissertations / Theses on the topic 'Site-Directed Mutagenesi'

Author: Grafiati

Published: 9 March 2023

Last updated: 19 July 2025

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1

Rúnarsdóttir, Saga. "Site-Directed Mutagenesis Studies of FucO." Thesis, Uppsala universitet, Institutionen för kemi - BMC, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235136.

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2

Al-Khatib, Haifa Yousef. "Site Directed Mutagenesis of Dienelactone Hydrolase." Thesis, University of North Texas, 1995. https://digital.library.unt.edu/ark:/67531/metadc277940/.

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The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.

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3

Chen, Wei 1965. "Site Directed Mutagenesis Of Dienelactone Hydrolase." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500900/.

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The role of individual amino acid residues of the enzyme dienelactone hydrolase was investigated. Using the polymerase chain reaction (PCR), a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHI to EcoRI clcD subclone in the plasmid pUC19. Site-specific mutants of dienelactone hydrolase were created using mismatched oligonucleotides to prime DNA synthesis. Specifically modified proteins from mutated clcD genes (Arg 81 to alanine, Tyr 85 to phenylalanine and Arg 206 to alanine), were encoded by the mutant clones. Enzyme assays showed that dienelactone hydrolase activity of

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4

Marc, Daniel. "La myristylation de la proteine de capside vp4 du poliovirus; son role dans le cycle viral." Paris 7, 1991. http://www.theses.fr/1991PA077059.

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La proteine de capside vp4 du poliovirus, ainsi que des precurseurs vp0 et p1, est myristlee: sa glycine n-terminale est liee de maniere covalente a un acide gras tetra-decanoique. Cette modification co-traductionnelle est determinee par la sequence n-terminale de la proteine (gly#1ala#2gln#3val#4ser#5ser#6). Par mutagenese dirigee a l'aide d'oligonucleotides, nous avons modifie dans le cadn viral la sequence codant pour le signal de myristylation de la proteine. Des transcrits genomiques portant les differentes mutations ont ete synthetises in vitro, et leurs proprietes analysees in vitro par

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5

White, Malcolm F. "Yeast phosphoglycerate mutase studied by site-directed mutagenesis." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/24419.

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6

Wang, Xiaoshan. "Site-Directed Mutagenesis in Francisella Tularensis by Allelic." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/36440.

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<p> Francisella tularensis is a Gram-negative, facultative intracellular coccobacillus and the etiologic agent of tularemia for a wide variety of vertebrate and invertebrate animal species. Several species and subspecies of Francisella are currently recognized. However, the majority of infections are caused by F. tularensis subspecies tularensis (type A) and subspecies holarctica (type B). Given the low infectious dose, multiple transmission routes, severity of illness, and lack of licensed vaccines, F. tularensis has long been considered a potential biological weapon and is now classified as

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7

Bowker-Kinley, Melissa M. "Pyruvate dehydrogenase kinase Kinetics, site-directed mutagenesis, and regulation /." [Bloomington, Ind.] : Indiana University, 2005. http://wwwlib.umi.com/dissertations/fullcit/3183930.

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Thesis (Ph.D.)--Indiana University, 2005.<br>Source: Dissertation Abstracts International, Volume: 66-07, Section: B, page: 3690. Chair: Robert A. Harris. Title from dissertation home page (viewed Oct. 5, 2006).

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8

Tito, Donald. "Site-directed mutagenesis of hydrogenase genes in Azotobacter chroococcum." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56889.

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Accessory hydrogen uptake genes have been identified in a region of the Azotobacter chroococcum genome about 5 kb downstream of the hydrogenase structural genes (hupSL). DNA sequencing has revealed six genes (hupABYCDE) in this region. These genes are probably transcribed in the same direction as hupSL but are probably in a different operon. Mutational analysis had shown that disruption of the hupB, hupY, hupD and hupE genes gives a Hup$ sp-$ phenotype. In the present work additional mutational analysis, using Tn5, a Tn5 -derivative containing a promoterless lacZ gene, and a kanamycin resistan

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9

Owegi, Margaret. "Site-directed mutagenesis of yeast V-ATPase subunit d." Virtual Press, 2005. http://liblink.bsu.edu/uhtbin/catkey/1319550.

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V-ATPases are enzymes found in all eukaryotic cells. They are organized into a peripheral membrane complex (V1) and an integral membrane complex (V0). VI is responsible for ATP hydrolysis and generates the energy used by Vo to pump protons from the cytosol into the vacuole. Subunit d is a component of Vo possibly located at the interface between V 1 and V. in the V-ATPase complex. We hypothesize that subunit d could be involved in the structural and functional coupling of VI and Vo. This was tested by generating point mutations along the open reading frame of subunit d from yeast. The mutation

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10

Schmidt, William Richard. "Site-directed mutagenesis of the ncd microtubule motor protein." Thesis, This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-12302008-063348/.

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11

Dou, Chao. "Site-directed mutagenesis as probes for F₁-ATPase function /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1997. http://wwwlib.umi.com/cr/ucsd/fullcit?p9812499.

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12

Patel, Jigar. "Site directed mutagenesis of L-pyrrolysine in dimethylamine methyltransferase." Connect to resource, 2009. http://hdl.handle.net/1811/36534.

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13

Merry, Stephen Alan Paul. "Exciton transfer and trapping in photosystem II." Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286500.

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14

CRIVELLO, PIETRO. "New molecular insights into HLA immunogenicity." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/29854.

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Alloreactivity is the major barrier to solid organ and hematopoietic stem cell transplantation (HSCT), determining important clinical events such as graft rejection and graft versus host disease. This biological phenomenon is due to the immunogenicity of allogeneic Human Leukocyte Antigens (HLA) expressed in transplanted tissues and recognized by T cell receptor (TCR) on the surface of alloreactive T cells. In the past, the hosting laboratory identified an immunogenic T cell epitope (TCE) encoded by a subset of HLA-DPB1 alleles, thereby defining permissive and non-permissive DPB1 mismatches be

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15

Blom, Lillemor. "Investigation of the interactions between the bacterial homologue to actin, and the chaperone GroEL/ES through a combination of protein engineering and spectroscopy." Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-15818.

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<p>Molecular chaperones help many proteins in the cell reach their native conformation. The mechanism with which they do this has been studied extensively, but has not been entirely elucidated. This work is a continuation of the study done by Laila Villebeck et al. (2007) on the conformational rearrangements in the eukaryotic protein actin in interaction with the eukaryotic chaperone TRiC. In this study the intentions were to analyze the protein MreB, a prokaryotic homologue to actin, when interacting with the prokaryotic chaperone GroEL. The purpose was to investigate if the mechanisms of Gro

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16

Aronica, Pietro. "Development of a new protocol for computatinal site-directed mutagenesis." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/40433.

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Mutagenesis, the technique of mutating individual amino acids on proteins and peptides, is an important part of protein engineering and analysis. By changing residues and measuring the effect of the mutation on the properties of the protein such as its structure and interaction, a deeper understanding can be gained, which can be used to design new, better biomolecules. However, when performed experimentally, mutagenesis can be expensive, time-consuming and a rate-limiting step in research. Computational tools can be used to aid within this context, but a review of existing methods revealed gap

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17

Lloyd, John S. "Heterologous expression and site-directed mutagenesis of soluble methane monooxygenase." Thesis, University of Warwick, 1997. http://wrap.warwick.ac.uk/59600/.

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The purpose of this investigation was to study the heterologous expression of soluble methane monooxygenase (sMMO) genes from Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3b. Using the T7-RNA polymerase expression system, the entire sMMO operon and subclones (constructed using the polymerase chain reaction) were over-expressed in E. coli. Results obtained using the Me. capsulatus (Bath) sMMO operon confirmed previous reports (C. West, G. P. C. Salmond, H. Dalton and 1. C. Murrell (1992). J Gen. Microbial. 138, 1301-1307) that functional expression of protein B and the reduc

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18

Chitpinityol, Supannee. "Heterologous expression and site-directed mutagenesis of the enzyme chymosin." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320101.

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19

Farr, George William. "Site-directed mutagenesis of beta tubulin's putative GTP-binding domain." Case Western Reserve University School of Graduate Studies / OhioLINK, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=case1060367517.

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20

Jack, Richard F. "Characterization and site-directed mutagenesis of NifU from Azotobacter vinelandii." Diss., This resource online, 1995. http://scholar.lib.vt.edu/theses/available/etd-10042006-143858/.

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21

Jonnalagadda, Madhuri. "Site Directed Mutagensis of Bacteriophage HK639 and Identification of Its Integration Site." TopSCHOLAR®, 2008. http://digitalcommons.wku.edu/theses/42.

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Bacteriophages affect bacterial evolution, pathogenesis and global nutrient cycling. They are also the most numerous and diverse group of biological entities on the planet [1, 2, 3, 4, 5, 6]. Members of the Lambda phage family share a similar genetic organization and control early gene expression by suppressing transcription, a process known as antitermination. Transcription antitermination in Lambda is mediated by a phage-encoded protein whereas in lambdoid phage HK022, antitermination is mediated by a phage-encoded RNA molecules. Recent results suggest that another bacteriophage called H

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22

Mizoguchi, Tadashi Jack Gray Harry B. Gray Harry B. Richards Jack. "Probing the role of the active-site Cysteine of Azurin by site-directed mutagenesis /." Diss., Pasadena, Calif. : California Institute of Technology, 1996. http://resolver.caltech.edu/CaltechETD:etd-01162009-111204.

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23

De, Villiers Jacques Izak. "Mutagenesis studies of a glycoside hydrolase family 2 enzyme." Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/98110.

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Thesis (MSc)--Stellenbosch University, 2015.<br>ENGLISH ABSTRACT: Galactooligosaccharides are produced by the transglycosylation activity of β-galactosidases (β-gal, EC 3.2.1.23) when utilising lactose as a substrate. They have emerged as important constituents used in the food and pharmaceutical industries owing to their prebiotic properties. Although transglycosylation was discovered in 1951 (Wallenfels 1951), and a number of β-gals have had their transglycosylation activity characterised, the activities of these enzymes are not optimal for industrial use. Their tendency to favour the hydrol

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24

Daniell, Sarah Jane. "Site-directed mutagenesis of the rat Dâ†2(Long) dopamine receptor." Thesis, University of Kent, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241618.

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25

Fan, Yan Baranger Anne M. Katzenellenbogen John A. Zhao Huimin Silverman Scott K. "Exploring protein-RNA interactions with site-directed mutagenesis and phage display." Urbana, IL.: University of Illinois, 2009. http://hdl.handle.net/2142/14755.

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26

Eriksson, Sollenberg Ulla. "Characterization of galanin receptors using chimeric peptides and site-directed mutagenesis /." Stockholm : Department of Neurochemistry, Stockholm University, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-37259.

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Diss. (sammanfattning) Stockholm : Stockholms universitet, 2010.<br>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 4: Manuscript. Härtill 4 uppsatser.

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27

Kaylor, Joanna Jacelyn. "Studies of the aggregation of [alpha]-synuclein using site-directed mutagenesis /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2004. http://uclibs.org/PID/11984.

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28

Butland, Gareth Paul. "Expression and site-directed mutagenesis of a bacterial nitric oxide reductase." Thesis, University of East Anglia, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327513.

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29

Man, Wai Jin. "Studies on the mechanism of citrate synthase using site directed mutagenesis." Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386673.

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30

Woodcock, Sarah Catherine. "Studies on the mechanism of porphobilinogen deaminase using site directed mutagenesis." Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385454.

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31

Carter, P. J. "Site-directed mutagenesis of the tyrosyl tRNA synthetase of Bacillus stearothermophilus." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372646.

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32

Burgess, J. G. "Construction of a system for site directed mutagenesis in Rhodobacter sphaeroides." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/47793.

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33

Smith, Thomas John. "β-lactamase of Staphylococcus aureus PC1 : studies using site-directed mutagenesis". Thesis, University of Edinburgh, 1992. http://hdl.handle.net/1842/11411.

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A system was developed for generating site-directed mutants of the β-lactamase of <i>Staphylococcus aureus</i> PC1, and for expression of the mutant proteins in <i>S.aureus</i>. The mutant genes were made <i>in vitro</i> using the polymerase catalysed chain reaction (PCR), by the overlap extension method (Ho, S.N. <i>et al</i>. [1989] <i>Gene</i> 77, 51-59). The were cloned in <i>Escherichia coli</i> and transferred to <i>S.aureus</i> using an <i>E.coli-S.aureus</i> shuttle vector. The mutant proteins were, like the wild-type, secreted into the medium by the transformed <i>S.aureus</i> cells.

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34

Caffrey, Michael Stephen. "Characterization of cytochrome c structure and function by site-directed mutagenesis." Diss., The University of Arizona, 1991. http://hdl.handle.net/10150/185346.

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A dual Rhodobacter capsulatus/Rhodobacter sphaeroides genetic system has been used to study the structure and function of R. capsulatus cytochrome c₂. In the first part of this study, the processing, stability and in vivo, functionality of nine site-directed mutants have been examined. Mutations were designed to test various structural and functional properties of cytochrome c₂ such as redox potential (Y75C, Y75F and Y75S), surface charges (K12D, K14E, K32E and K14E/K32E), and protein conformation (P35A and W67Y). All R. capsulatus cytochrome c₂ mutants, except Y75C and Y75S, were overproduced

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35

Setterquist, Robert Alan. "Site-directed mutagenesis of the nitrogenase MoFe protein from Azotobacter vinelandii." Thesis, Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/50091.

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A model describing the potential amino acid ligands to the four 4Fe-4S centers (P-clusters) within the Azotobacter vinelandii nitrogenase MoFe protein is presented. Based on interspecies and intersubunit amino acid comparisons of the α- and ß-subunits of the MoFe protein, and the FeMoco biosynthetic proteins, NifE and NifN, four conserved residues (Cys62, His83, Cys88, Cys154 all proposed P-cluster ligands) within the α- subunit were targeted for site-directed mutagencsis studies. In order to define a range of acceptable substitutions, 35 specific site-mutants have been constructed, each with

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36

Khaja, Sara. "Site-Directed Mutagenesis in Citrus paradisi Flavonol-Specific 3-O-Glucosyltransferase." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etd/2453.

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Flavonoids are plant secondary metabolites that have significant biochemical and physiological roles. Biosynthesis of these compounds involves several modifications, most predominantly glucosylation, which is catalyzed by glucosyltransferases (GTs). A signature amino acid sequence, the PSPG box, is used to identify putative clones and has been shown to be involved in UDP-glucose binding. Site-directed mutagenesis is used to answer questions regarding the structure and function of this family of enzymes, particularly what allows some GTs to be more selective towards some substrates than others.

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37

Duncan, Tammi Rae. "Site-directed mutagenesis of the SPOR domain from Escherichia Coli FtsN." Thesis, University of Iowa, 2011. https://ir.uiowa.edu/etd/954.

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Escherichia coli cell division is a complex process involving over 30 proteins that are recruited to the division site. One of the important division proteins is FtsN, which has a C-terminal peptidoglycan (PG) binding region called the SPOR domain. Most SPOR domain proteins are probably involved in bacterial cell division, but their precise role in this process is not known. Although the structure of the FtsN SPOR domain has been solved by NMR, nothing is known about how the domain binds PG. Understanding the SPOR:PG interaction is important because it could lead to novel insights into PG meta

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38

Sheng, Mei. "Site Directed Mutagenesis of β-Ketoadipate Succinyl-Coenzyme A Transferase II from Acinetobacter Calcoaceticus". Thesis, University of North Texas, 1993. https://digital.library.unt.edu/ark:/67531/metadc279281/.

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The role of specific amino acid residues in β-ketoadipate succinyl-coenzyme A transferase II from Acinetobacter calcoaceticus was investigated. A 1412 base pair BamiHI-EcoRI fragment carrying the catIJ genes was amplified by polymerase chain reaction and inserted into pUCl9 to generate the plasmid pCATEl9. Escherichia coli DH5α (pCATEl9) carrying only the catlJ genes expressed 3-fold higher enzyme activity than the parent strain. Two mutants were constructed by site directed mutagenesis so that glutamate was replaced by a glutamine at positions Gln155 and Gln193 in the ß subunit of the primary

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39

De, Nagornoff Leititia. "Site-directed mutagenesis of active-site residues in the hydroxylase component of soluble methane monooxygenase." Thesis, University of Warwick, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439651.

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40

Temperley, Richard James. "Generation of a reporter for mitochondrial gene expression studies." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369782.

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41

Gossner, Anton Gerhard. "Bio-engineering and genetic manipulation of ovine interleukin-2." Thesis, University of the West of England, Bristol, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297877.

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42

陳家輝 and Ka-fai Joseph Chan. "Production of novel biological proteins by hybridoma technique and site directed mutagenesis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1993. http://hub.hku.hk/bib/B31211148.

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43

Chan, Ka-fai Joseph. "Production of novel biological proteins by hybridoma technique and site directed mutagenesis /." [Hong Kong] : University of Hong Kong, 1993. http://sunzi.lib.hku.hk/hkuto/record.jsp?B1364130X.

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44

Shi, Qingli. "Expression and site-directed mutagenesis studies of a ribosome-inactivating protein : neo-trichosanthin." HKBU Institutional Repository, 1999. http://repository.hkbu.edu.hk/etd_ra/221.

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45

Arnett, Diana. "Site-Directed Mutagenesis of the -127 Activator Binding Site of the qa-2 Gene of Neurospora crassa." Youngstown State University / OhioLINK, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1004634030.

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46

Delavari, Azar. "Structure-activity investigation on laccases by computational and site directed mutagenesis studies." Doctoral thesis, Universitat Politècnica de Catalunya, 2016. http://hdl.handle.net/10803/404452.

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Laccases belong to multi copper oxidase enzyme family (EC 1.10.3.2). Their capacity to oxidíze a wide range of substrates makes them very attractive for the industry and are growing in importance for environmentally-friendly synthesis. Laccases have three different copper sites including, type 1 (T1), type 2 (T2) and type 3 (T3). The function of the T1 site is shuttling electrons from the substrate to the trinuclear copper cluster. During the catalytic cycle of laccase, four electrons are removed from four substrate molecules, which are finally transferred to reduce oxygen to two water molecu

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47

Taylor, John Michael. "Revealing flagellar motor protein interactions by site directed mutagenesis in R. sphaeroides." Thesis, Nottingham Trent University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.444661.

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48

Gartland, Martin John. "Site-directed mutagenesis of the aromatic amino acid aminotransferase of Escherichia coli." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293014.

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49

Liu, Hsiao-Hui. "EcoRV endonuclease studied by site-directed mutagenesis and oligodeoxynucleotides containing modified bases." Thesis, University of Newcastle Upon Tyne, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287807.

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50

Wang, Xing-Guo. "Alteration of substrate specificity in clostridial glutamate dehydrogenase by site-directed mutagenesis." Thesis, University of Sheffield, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387762.

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