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Dissertations / Theses on the topic 'SITE MUTATION'

Author: Grafiati

Published: 11 September 2023

Last updated: 31 July 2025

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1

Patel, Premal Harshad. "Evolution of DNA polymerase active site /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/6361.

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2

Marcello, Matthew R. "Analysis of recombinant human prostasin carrying a serine active site mutation." Honors in the Major Thesis, University of Central Florida, 2003. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/325.

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This item is only available in print in the UCF Libraries. If this is your Honors Thesis, you can help us make it available online for use by researchers around the world by following the instructions on the distribution consent form at http://library.ucf.edu/Systems/DigitalInitiatives/DigitalCollections/InternetDistributionConsentAgreementForm.pdf You may also contact the project coordinator, Kerri Bottorff, at kerri.bottorff@ucf.edu for more information.<br>Bachelors<br>Health and Public Affairs<br>Molecular Biology and Microbiology

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3

Wenwieser, Sandra Verena Corinna Tina. "Subunit interactions in regulation and catalysis of site-specific recombination." Thesis, University of Glasgow, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343974.

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4

Chitpinityol, Supannee. "Heterologous expression and site-directed mutagenesis of the enzyme chymosin." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320101.

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5

Sheikh, Qaiser Iftikhar. "Exploring the structure and function of bacterial cytosine specific DNA methyltransferases using site-directed mutagenesis." Thesis, University of Sheffield, 2001. http://etheses.whiterose.ac.uk/10258/.

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Point mutations were engineered into the sequence of the multispecific DNA methyltransferase (Mtase) M. SPRI in motif IX, in order to mimic the corresponding motif IX of mono-specific Mtase. A similar approach was adopted to modify the sequence of the monospecific enzyme M. HhaI in motifs IX and X based on the available structure and as a consequence the enzyme regained methylation potential. It was thought that these changes might be sufficient to enable functional exchange of the target recognition domains (TRDs) between a mono- and a multispecific enzyme. However, insertion of various segme

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6

Dinda, Stephen B. "Predicting RNA Mutation Using 3D Structure." Bowling Green State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1321280932.

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7

Tinteroff, Gil Vanessa. "De Paracas à Nasca sur la côte du sud du Pérou : archéologie d'une mutation culturelle." Paris 4, 2008. http://www.theses.fr/2008PA040039.

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Sur la côte sud du Pérou, à l'issu du déclin de la civilisation Chavín, la culture Paracas laisse peu à peu place à la culture Nasca. Datée entre 200 avant J. -C. Et 100 de notre ère, cette période, communément appelée "transition Paracas – Nasca" sur la côte sud, est celle de nombreux changements culturels. À travers l’étude de divers contextes archéologiques de cette région, et en particulier de Necrópolis dans la péninsule de Paracas et de Cahuachi dans la vallée de Nasca, cette thèse se propose de définir les causes, les mécanismes socioculturels et les acteurs culturels des transformation

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8

Lefebvre, Anne. "Le site CpG dans l'ADN : impact possible des variations conformationnelles sur le taux de mutations." Châtenay-Malabry, Ecole centrale de Paris, 1996. http://www.theses.fr/1996ECAP0485.

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Nous avons analysé la structure du dinucléotide CpG en fonction de la séquence d'ADN qui le contient, par résonance magnétique nucléaire et modélisation moléculaire, pour déterminer en quoi la structure de CpG influe sur les modifications structurales induites par la méthylation de la cytosine de ce dinucléotide, et mettre en relation la structure de CpG et le taux de mutations observé sur ces sites. La permutation de ses plus proches voisins modifie fortement la conformation de CpG. Au sein de la tétrade ACGT, comme dans d(GTACGTAC)2, il adopte un grand twist, associé à une phase élevée de la

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9

Sada, Yoshinao. "Genetic studies on the target-site resistance to sulfonylurea herbicides in Schoenoplectus juncoides." Kyoto University, 2014. http://hdl.handle.net/2433/193553.

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10

Liu, Fengling. "Kinetic and Crystallographic Studies of Drug-Resistant Mutants of HIV-1 Protease: Insights into the Drug Resistance Mechanisms." Digital Archive @ GSU, 2007. http://digitalarchive.gsu.edu/biology_diss/19.

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HIV-1 protease (PR) inhibitors (PIs) are important anti-HIV drugs for the treatment of AIDS and have shown great success in reducing mortality and prolonging the life of HIV-infected individuals. However, the rapid development of drug resistance is one of the major factors causing the reduced effectiveness of PIs. Consequently, various drug resistant mutants of HIV-1 PR have been extensively studied to gain insight into the mechanisms of drug resistance. In this study, the crystal structures, dimer stabilities, and kinetics data have been analyzed for wild type PR and over 10 resistant mutants

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11

Mei, Xiaonan. "HOW A SILENT MUTATION SUPPRESSES THE ACTIVITY AND IRON INCORPORATION IN SUPEROXIDE DISMUTASE." UKnowledge, 2012. http://uknowledge.uky.edu/chemistry_etds/9.

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A mutation (CTG to TTG) of FeSOD gene was found in Escherichia coli. Since they both encode leucine, it is a silent mutation. Site-‐directed mutagenesis was applied to correct the mutation, and the mutant FeSOD (before gene correction) and wild type FeSOD (after gene correction) were purified. The FeSODs from the two genes were Characterized using different assays and spectroscopic methods including EPR and CD. The requirement for the rare codon TTG may result in slowed translation and heavy demand on a scarce tRNA. Cultures expressing wild type FeSOD are better able to grow for long times af

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12

Lowes, Damon Anthony. "Resolution of DNA adduct formation at the nucleotide level and correlation with site specific propensity to mutation." Thesis, University of Leicester, 2002. http://hdl.handle.net/2381/30766.

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Tamoxifen, a non steroidal antioestrogen (Z-trans-1-[4-(dimethylaminoethoxy)phenyl]-1,2-diphenyl-1-butene), is widely used in the treatment of breast cancer, and is undergoing clinical evaluation as a chemopreventative in women thought to be at high risk of developing the disease. Tamoxifen is hepatocarcinogenic in rats, forming large numbers of tamoxifen DNA adducts when dosed over a period of time, but is inactive in standard genotoxicity tests. In this project I determined in vitro and in vivo DNA adduct formation at the nucleotide level from tamoxifen and selected metabolites. Sites of tam

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13

Pekgoz, Gulsah. "Deletion Mutation Of Glnb And Glnk Genes In Rhodobacter Capsulatus To Enhance Biohydrogen Production." Master's thesis, METU, 2010. http://etd.lib.metu.edu.tr/upload/12612677/index.pdf.

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Rhodobacter capsulatus is a photosynthetic, purple non-sulfur (PNS) bacterium that produces biohydrogen via photofermentation. Nitrogenase enzyme is responsible for hydrogen production<br>during fixation of molecular nitrogen into ammonium, hydrogen is produced. Since this process is an energetically expensive process for the cell, hydrogen production is strictly controlled at different levels. When ammonium is present in the environment, hydrogen production completely ceases. The key proteins in the regulation of nitrogenase by ammonium are two PII proteins<br>GlnB and GlnK. &lsquo<br>Hyvolu

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14

O'Neill, Jason Charles Walker. "Structural studies on the B1 domain of protein L : biophysical affects of single site mutations, 3D-domain swapping, and computational redesign /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/4990.

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15

Valdivia, Karina. "Caractérisation du phénotype associé à la mutation masculinisante "mal" chez la truite arc-enciel, Oncorhynchus mykiss." Phd thesis, Rennes 1, 2012. https://ecm.univ-rennes1.fr/nuxeo/site/esupversions/187e2221-ddee-41d5-8f81-4a7b17551a27.

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La truite arc-en-ciel a un déterminisme du sexe strictement génétique de type XX/XY. Pourtant des femelles génétiques se sont spontanément masculinisées après une gynogénèse endomitotique. Ce phénotype masculinisé à pénétrance incomplète serait dû à une mutation inconnue appelée « mal ». Afin d'identifier le gène causal porteur de cette mutation, une meilleure caractérisation de son phénotype est nécessaire. Pour cela, nous avons procédé à la caractérisation du phénotype gonadique en utilisant à la fois des approches histologiques et moléculaires. Tous les animaux XXmal présentent une différen

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16

Scherperel, Gwynyth. "Characterization of the sequence and substrate reactivity of dihydroneopterin aldolase and its site-directed mutants by tandem mass spectrometry." Diss., Connect to online resource - MSU authorized users, 2006.

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17

Taylor, Russell Haywood. "A guanine to adenine mutation -76bp from the transcriptional start site decreases constitutive CYP1A2 expression in a novel mouse strain." Thesis, University of Ottawa (Canada), 2007. http://hdl.handle.net/10393/27924.

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Variations in the expression of drug metabolizing enzymes of the Cytochrome P450 superfamily are a principal cause of atypical reactions to therapeutics. The molecular mechanisms by which the metabolizing enzymes of the drug are regulated, and the effects of genetic variation on this regulation, are not completely understood. Cytochrome P450 1A2 (CYP1A2) is one such enzyme. The APN mouse strain has low expression of the CYP1A2 enzyme, relative to the C3H/HeJ strain. It was hypothesized that this difference in expression of the CYP1A2 was occurring as a result of a single nucleotide polymorphis

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18

Haddi, Khalid. "Studies on insecticide resistance in tuta absoluta (Meyrick), with special emphasis on characterisation of two target site mechanisms." Doctoral thesis, Università di Catania, 2012. http://hdl.handle.net/10761/1226.

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Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is a primary pest of tomato plants and is native to South America. Since the first documented European case in 2006, it has spread throughout the Mediterranean basin and North Africa. Larval stages cause direct feeding damage and reductions to both yield and fruit quality. Chemical insecticides have been the main control tools used against T. absoluta, but decreasing efficacy has been attributed to the development of insecticide resistance. During this study, leaf-dip bioassays were used to quantify responses of five field strains of T. absolut

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19

Park, Sung-Hoon. "Expression and characterization of an extremely thermostable Beta Glycosidase (Mannosidase) from the hyperthermophilic Aracheon Pyrococcus Furiosus DSM3638 and mutation studies in the active site." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97062.

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Genomic analysis of the hyperthermophilic archaeon Pyrococcus furiosus revealed the presence of an open reading frame (ORF PF0356) similar to the enzymes in glycoside hydrolase family 1. This beta-glycosidase, designated PFTG (Pyrococcus furiosus thermostable glycosidase), was cloned and expressed in Escherichia coli. The expressed enzyme was purified by heat treatment and Ni-NTA affinity chromatography. The gene was composed of 1452 bp encoding 483 amino acids for a protein with a predicted molecular mass of 56,326 Da. The temperature and pH optima were 100°C and 5.0 in sodium citrate buffer,

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20

Bouchart, Franck. "Impact des mutations OPG chez Erwinia chrysanthemi : recherche de suppresseurs et analyse protéomique des mutants." Lille 1, 2006. https://ori-nuxeo.univ-lille1.fr/nuxeo/site/esupversions/df8106c9-51b3-47fd-9d4c-9aba9e38f18e.

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Erwinia chrysanthemi est une entérobactérie phytopathogène pectinolytique faisant partie de la subdivision γ des protéobactéries. Elle infecte un large spectre de plantes hôtes engendrant la pourriture molle. L'apparition de cette pourriture molle dépend de la synthèse et de la sécrétion d'une batterie d'exoenzymes (cellulases, protéases et pectate-Iyases) capable de dégrader les composants de la paroi des cellules végétales. Néamnoins, l'expression de nombreux autres gènes est nécessaire à la virulence de cette bactérie. En effet, les mutants déficients en glucanes périplasmiques osmorégulés

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21

Radev, Zlatko. "Site-directed nucleases as tools for genome editing in fish." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112422.

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L'application des techniques de séquençage à haut débit dans les dernières années a conduit à l'obtention de la séquence de génomes complets de plusieurs organismes. Le développement de nouveaux outils de génétique inverse était donc souhaitable afin de faire un usage optimal des données accumulées. Les nucléases hautement spécifiques représentent un outil unique pour induire des modifications ciblées du génome in vivo. L'induction d'une cassure double brin dans l'ADN est réparée soit par la voie de jonction d’extrémités nonhomologues soit par la voie très fidèle de la recombinaison homologue.

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22

Borgato, Ednaldo Alexandre. "Identificação de Amaranthus palmeri, caracterização da resistência múltipla a herbicidas inibidores da ALS e da EPSPS e controle químico baseado no uso das novas tecnologias transgênicas." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-16052018-125757/.

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A planta daninha Amaranthus palmeri é nativa dos Estados Unidos, porém foi pela primeira vez relatada no Brasil no ano de 2015. Embora comprovadamente com resistência múltipla aos herbicidas inibidores da ALS e da EPSPS, até o momento não foram investigadas as bases moleculares da resistência. Além disso, por causa da recente introdução da planta daninha no país, alternativas de manejo com culturas tolerantes a herbicidas necessitam ser estudadas. Sendo assim, os objetivos desse trabalho são de caracterizar a espécie de planta daninha introduzida no país, identificar os mecanismos de resistênc

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23

Simões, Sarah Capelupe. "Caracterização bioquímica e farmacológica de receptores AT1 de angiotensina II contendo mutações relacionadas à fibrilação atrial em humanos." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/17/17131/tde-01022016-152915/.

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Os receptores acoplados à proteína G (GPCRs) são proteínas integrais de membrana caracterizados por possuírem sete alfa-hélices transmembranares. Esses receptores são importantes alvos de estudos biomédicos e aproximadamente 40% dos medicamentos atualmente comercializados agem sobre estes receptores. O receptor de Angiotensina II do tipo 1 (AT1) é um GPCR e o principal mediador do Sistema Renina-Angiotensina que tem como principal efetor o octopeptídeo Angiotensina II (AngII). Recentemente foi descrito que as mutações A244S e I103T-A244S no receptor AT1 podem estar relacionadas com a predispos

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24

Smith, Adam N. "Reduced Chemical Weed Control Options in Virginia for Corn and Turfgrass and Characterization of Sorghum halepense Expressing Multiple Resistance to Nicosulfuron and Glyphosate." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/56957.

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Sustainable weed control in managed agricultural systems requires the judicious use of multiple weed control tactics and prevents over-reliance on any one tactic. In this context, sustainable weed management plays a critical role in the mitigation of one of agriculture's most pressing problems- herbicide resistance. Research conducted in Virginia sought to explore the effects of integrating multiple weed management tactics in corn and cool-season turfgrass. Additionally, research was conducted to confirm nicosulfuron and glyphosate herbicide resistance in Virginia johnsongrass and elucidate th

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25

Mazza, Catherine. "17-hydroxystéroïde déshydrogénase humaine de type I : analyse des relations structure-fonction par mutagenèse dirigée et cristallographie des rayons X." Université Joseph Fourier (Grenoble ; 1971-2015), 1997. http://www.theses.fr/1997GRE10214.

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La 17-hydroxysteroide deshydrogenase de type 1 (17-hsd1) est responsable de la synthese de l'stradiol, strogene biologiquement actif implique dans le declenchement et la proliferation des cancers du sein hormono-dependants. Cette enzyme appartient a la famille des deshydrogenases reductases a chaine courte dans laquelle les residus ser142 tyr155 et lys159, tres conserves, sont supposes intervenir dans le transfert de proton. La 17-hsd1 presente une specificite exclusive, mais inexpliquee vis-a-vis des steroides possedant un cycle a aromatique. De plus, elle utilise, in vitro, aussi bien le nad

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26

Hegazy, Usama M. "Structure-Function Relationships of Pi Class Glutathione Transferase Studied by Protein Engineering." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7146.

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27

Galindo, ramirez Martha Liliana. "Un monde en mutation : jeunesse, internet et politique : les cas du mouvement étudiant MANE en Colombie et du mouvement Acampa Sampa Ocupa Sampa au Brésil : (2011)." Thesis, Université Grenoble Alpes (ComUE), 2016. http://www.theses.fr/2016GREAH033.

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Cette thèse analyse les transformations des pratiques politiques des jeunes liées à l’usage d’Internet, en particulier de Facebook, en 2011 dans le cas du mouvement pour l’éducation Mane -Mesa Amplia Nacional Estudiantil- en Colombie et du mouvement d’occupation Acampa Sampa Ocupa Sampa au Brésil. Ce travail intègre des entretiens, l’élaboration de bases de données issues des pages Facebook et l’étude des dynamiques en ligne et hors ligne en soulevant des singularités et des enjeux méthodologiques. Il examine des modalités d’appropriation de Facebook ainsi que le statut de la jeunesse, les rap

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28

Cavallin, Mara. "Physiopathologie moléculaire et cellulaire des anomalies du développement du cortex cérébral : le syndrome d'Aicardi WDR81 mutations cause extreme microcephaly and impair mitotic progression in human fibroblasts and Drosophila neural stem cells TLE1, a key player in neurogenesis, a new candidate gene for autosomal recessive postnatal microcephaly Mutations in TBR1 gene leads to cortical malformations and intellectual disability Aicardi syndrome: Exome, genome and RNA-sequencing of a large cohort of 19 patients failed to detect the genetic cause Recurrent RTTN mutation leading to severe microcephaly, polymicrogyria and growth restriction Recurrent KIF2A mutations are responsible for classic lissencephaly Recurrent KIF5C mutation leading to frontal pachygyria without microcephaly Rare ACTG1 variants in fetal microlissencephaly De novo TUBB2B mutation causes fetal akinesia deformation sequence with microlissencephaly: An unusual presentation of tubulinopathy A novel recurrent LIS1 splice site mutation in classic lissencephaly Further refinement of COL4A1 and COL4A2 related cortical malformations Prenatal and postnatal presentations of corpus callosum agenesis with polymicrogyria caused By EGP5 mutation Delineating FOXG1 syndrome from congenital microcephaly to hyperkinetic encephalopathy Delineating FOXG1 syndrome: From congenital microcephaly to hyperkinetic encephalopathy." Thesis, Sorbonne Paris Cité, 2019. https://wo.app.u-paris.fr/cgi-bin/WebObjects/TheseWeb.woa/wa/show?t=2213&f=18201.

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Les malformations du cortex cérébral (MDC) représentent une cause importante de handicap et d'épilepsie pharmaco-résistante. Le séquençage à haut débit a permis une amélioration considérable de l'identification des bases moléculaires des MDC non syndromiques. Toutefois, certaines formes, notamment les MDC complexes, demeurent inexpliquées. Mon projet de thèse a pour objectif de progresser dans la compréhension des MDC complexes en utilisant deux modèles : les microlissencéphalies (MLIS) et le syndrome d'Aicardi (AIC), une forme syndromique particulière associant des malformations de l'oeil et

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29

Plantier, Jean-Luc. "La thrombine dans la physiopathologie vasculaire : une étude structure-fonction." Université Joseph Fourier (Grenoble), 1994. http://www.theses.fr/1994GRE10179.

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La thrombine est une glycoproteine de la famille des proteases a serine. Comparee a celles des autres membres de sa famille, sa structure tertiaire possede une serie de boucles d'insertion particulierement exposees, organisees autour du site actif. Parce que la specificite restreinte de la thrombine pour ses substrats semble faire appel a des interactions a la surface de la molecule, il nous est apparu interessant de determiner quels etaient les roles de ces boucles dans certaines des activites de l'enzyme. Des mutations ponctuelles ont ete realisees dans deux de ces boucles (la boucle b et la

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30

Kim, Kyung-Sun. "Mutation, migration, dissémination dans le travail in situ." Paris 1, 2002. http://www.theses.fr/2002PA010656.

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C'est à un grand voyage que je convie le lecteur à travers ma recherche, entre deux continents, l'Orient et l'Occident. Mais c'est aussi d'un cheminement labyrinthique dont j'essaie de témoigner. Cheminement au cours duquel j'ai fait des rencontres inattendues et des découvertes surprenantes sur moi-même. Cet aller et retour continuel entre la France et la Corée, a engendré puis alimenté des pratiques artistiques nouvelles. N me fallait parler ici et là-bas, de trois éléments symboliques. L'algue, le papier et la grue. Au-delà de la langue, c'est à travers l'espace plastique que j'ai choisi de

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31

Eöry, Lél. "Inferring strength of selection in vertebrate genomes." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/4925.

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Protein-coding sequences have long been assumed to evolve under selection, but the quantification of the process at the nucleotide sequence level only started when a simple null model, the neutral theory of molecular evolution, was formulated by Kimura. Several methods were developed, which were based on the assumption that synonymous sites (nucleotides at third codon positions which do not change the encoded amino acid) evolve close to neutrally, and could be used as local neutral standards. Most of our current knowledge on the direction and strength of selection still depends on this simple

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32

Chen, Wei 1965. "Site Directed Mutagenesis Of Dienelactone Hydrolase." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500900/.

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The role of individual amino acid residues of the enzyme dienelactone hydrolase was investigated. Using the polymerase chain reaction (PCR), a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHI to EcoRI clcD subclone in the plasmid pUC19. Site-specific mutants of dienelactone hydrolase were created using mismatched oligonucleotides to prime DNA synthesis. Specifically modified proteins from mutated clcD genes (Arg 81 to alanine, Tyr 85 to phenylalanine and Arg 206 to alanine), were encoded by the mutant clones. Enzyme assays showed that dienelactone hydrolase activity of

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33

Kiflemariam, Sara. "Expression and Mutation Analyses of Candidate Cancer Genes In Situ." Doctoral thesis, Uppsala universitet, Institutionen för immunologi, genetik och patologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-184510.

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Cancers display heterogeneity in genetic profiles of the individual cancer cells and in the composition of different malignant and non-malignant cell populations. Such intra-tumor heterogeneity plays a role in treatment response and the emergence of resistance to cancer therapies. Approaches that address this complexity and improve stratification of patients for treatment are therefore highly warranted. Thus, the aims of this thesis were to further develop and apply in situ technologies for expression and mutation analyses of candidate cancer genes to gain a deeper understanding of cancer biol

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34

Rehmani, Imran J. "Studying the DNA Binding and Conformation of Metal-Binding Site Mutations in Pirin." Digital Archive @ GSU, 2012. http://digitalarchive.gsu.edu/chemistry_theses/53.

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The transcription factor NF-κB interacts with many other co-regulator proteins that modulate its binding and transcriptional activity. One of these co-regulators, Pirin, is an iron-dependent metalloprotein that has been shown to enhance the DNA binding of NF-κB homodimers. Here, we characterize the interactions between Pirin and its known NF-κB binding partners and examined the role of Bcl-3, a protein that is required for Pirin’s interaction with p50. In addition, we use site-directed mutagenesis to alter conserved residues within Pirin’s metal binding environment and observed how it affected

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35

Mathu, Alexander Muchugia Nganga. "Structural analysis of effects of mutations on HIV-1 subtype C protease active site." Thesis, Rhodes University, 2012. http://hdl.handle.net/10962/d1004073.

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HIV/AIDS is a global pandemic that poses a great threat especially in Sub-Saharan Africa where the highest population of those infected with the virus is found. It has far reaching medical, socio-economic and scientific implications. The HIV-1 protease enzyme is a prime therapeutic target that has been exploited in an effort to reduce morbidity and mortality. However problems arise from drug toxicity and drug-resistant mutations of the protease which is a motivation for research for new, safer and effective therapies. Evidence exists to show that there are significant genomic differences in Su

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36

FLORENTZ, EGELE CATHERINE. "L'extremite 3'oh aminoacyable du rna du virus de la mosaique jaune du navet : relations entre structure et fonctions." Université Louis Pasteur (Strasbourg) (1971-2008), 1987. http://www.theses.fr/1987STR13181.

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Analyse structurale (structure secondaire et tertiaire) d'un fragment contenant la sequence "trna like" (extremite 3' oh possedant plusieurs caracteristiques d'un arn de transfert). Modelisation de la structure sur ecran graphique. Analyse des zones de contact entre ce fragment d'arn et la valyl-trna synthetase, a l'aide de differentes sondes structurales. Discussion sur le role de l'extremite "trna like" dans le cycle de developpement du virus (regulation de la traduction de l'information genetique portee par l'arn viral)

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37

Sundström, Hannah. "Mutation and Diversity in Avian Sex Chromosomes." Doctoral thesis, Uppsala University, Department of Evolutionary Biology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3732.

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<p>Sex chromosomes are useful for the study of how factors such as mutation, selection, recombination and effective population size affect diversity and divergence.</p><p>A comparison of gametologous introns in seven different bird species revealed a complete lack of diversity on the female-specific W chromosome. In contrast, Z had at least one segregating site in all examined species. This can be explained by the lower mutation rate and lower effective population size of W but also suggests that selection affects diversity levels on the non-recombining W chromosome.</p><p>In a diverse set of

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Devaiah, Shivakumar P., and Cecelia A. McIntosh. "Site-Directed Mutational Analysis of Flavonol 3-0-Glucosyltransferases from Citrus paradisi." Digital Commons @ East Tennessee State University, 2013. https://dc.etsu.edu/etsu-works/340.

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Glucosyltransferases (GTs) are the important group of enzymes which facilitates the incorporation of UDPactivated glucose to a corresponding acceptor molecule through glucosylation. Glucosylation is a common alteration reaction in plant metabolism and is regularly associated with the production of secondary metabolites. Glucosylation serves a number of roles within metabolism including: stabilizing structures, affecting solubility, transport, and regulating the bioavailability of the compounds for other metabolic processes. GTs involved in secondary metabolism share a conserved 44 amino acid r

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Grundberg, Ida. "Genotyping and Mutation Detection In Situ : Development and application of single-molecule techniques." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-149776.

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The human body is composed of trillions of cells closely working together to maintain a functional organism. Every cell is unique in molecular composition and can acquire genetic variations that might cause it to turn pathological. It is essential to develop improved tools to better understand the development of normal and disease tissue, ideally enabling single-cell expression studies in preserved context of complex tissue with single-nucleotide resolution. This thesis presents the development and application of a new in situ method for localized detection and genotyping of individual transcr

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Sanders, Stephen Anthony. "The effect of selected active site mutations on the properties of horseradish peroxidase isoenzyme C." Thesis, University of Sussex, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357651.

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Hersh, Megan N. "VISUALIZING GENOMIC INSTABILITY: IN SITU DETECTION AND QUANTIFICATION OF MUTATION IN MICE." University of Cincinnati / OhioLINK, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ucin991312483.

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Schmitz, Stephan. "Mutational analysis of proposed myosin binding sites on actin." Thesis, University of York, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301148.

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Ajlani, Ghada. "Détermination des sites de mutation responsables de résistance aux herbicides chez des mutants de la cyanobactérie Synechocystis PCC 6714 : étude de l'effet de ces mutations sur le transfert d’électrons du photosystème II." Paris 11, 1989. http://www.theses.fr/1989PA112130.

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Li, Zhen. "Structural and functional consequences of single mutations at the high affinity binding site of cyanovirin-N." Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/3133.

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This thesis focuses mainly on the consequences that single mutations have on structural, functional and energetic aspects of the protein cyanovirin-N. In order to estimate the free energy of single mutations, we have applied thermodynamics integration and Bennett acceptance ratio techniques. Replica exchange molecular dynamics has been applied to accelerate simulations for complicated scenarios. Our studies suggest that certain single mutations may be promising to improve binding affinity to Manα1→2Manα but we also learned that the simplistic view of a strong hydrogen bond correlating to a hig

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Crandall, Jacob N. "Ribosomal RNA Mutations that Inhibit the Activity of Transfer-Messenger RNA of Stalled Ribosomes." Diss., CLICK HERE for online access, 2010. http://contentdm.lib.byu.edu/ETD/image/etd3535.pdf.

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Suleman, Essa. "Mutational analysis of the PacC binding sites within the aflR promoter in Aspergillus flavus." Thesis, Nelson Mandela Metropolitan University, 2011. http://hdl.handle.net/10948/d1012683.

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It is generally known that media containing simple sugars (sucrose, glucose) and organic nitrogen sources (ammonium) when buffered to acidic pH stimulates aflatoxin production in Aspergillus flavus & A. parasiticus while lactose, nitrate and an alkaline pH inhibit aflatoxin biosynthesis. It has been shown that pH of the growth medium is the most important regulatory factor for aflatoxin biosynthesis since media containing stimulatory carbon and/or nitrogen sources (sucrose and ammonia) do not enhance aflatoxin (or sterigmatocystin) production at alkaline pH. RNA interference (in A. flavus) of

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Beal, Brian D. "UV-induced mutations at a non-dimer site in E. coli : possible role for a TA* photoproduct /." Available to subscribers only, 2007. http://proquest.umi.com/pqdweb?did=1400950891&sid=1&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Thesis (M.S.)--Southern Illinois University Carbondale, 2007.<br>"Department of Molecular Biology, Microbiology and Biochemistry." Includes bibliographical references (leaves 67-74). Also available online.

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Kergourlay, Virginie. "Mise au point d'outils novateurs pour l'identification de mutations pathogènes : le cas des dysferlinopathies." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM5044.

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Le diagnostic des maladies génétiques est difficile à émettre. En effet, il est souvent difficile de déterminer comment une mutation va entrainer la pathologie. Le but de cette thèse est de développer des outils permettant de répondre à cette interrogation. Les mutations peuvent entrainer des anomalies à différents niveaux, différentes outils ont ainsi été développées en parallèle afin de pouvoir détecter différents types d'anomalies. Ces outils ont été développés en utilisant comme modèle une maladie génétique appartenant à la famille des myopathies, entrainant une dégénérescence des muscles

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Seedy, Ayman Salah Ahmed El. "Études moléculaire et cellulaire des mutations du gène CFTR : de la génétique à la fonctionnalité de la protéine." Poitiers, 2011. http://nuxeo.edel.univ-poitiers.fr/nuxeo/site/esupversions/1f04df89-c6ec-4a24-a424-49e80e1ea1a4.

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La mucoviscidose est la maladie génétique grave à transmission autosomique récessive la plus fréquente dans les populations d'origine européenne. Cette pathologie est due au dysfonctionnement de la protéine CFTR (Cystic Fibrosis Transmembrane conductance Regulator), canal chlorure présent à la membrane apicale des cellules épithéliales. La gravité de la pathologie dépend de la ou des mutations du gène CFTR. L’objectif de nos travaux est de comprendre l’impact des mutations du gène CFTR afin d’établir un conseil génétique avisé. Pour cela, nous avons dans un premier temps déterminé quelles sont

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Kong, Huanjun. "Engineering of benzene-1,3,5-tricarboxamide supramolecular polymers through subtle atom/group mutation in their side chain." Electronic Thesis or Diss., Sorbonne université, 2025. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2025SORUS107.pdf.

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Les molécules de type benzène-1,3,5-tricarboxamide (BTA) sont largement utilisées pour la formation de polymères supramoléculaires hélicoïdaux combinant liaisons hydrogène et interactions π-π. Cette thèse explore comment des modifications subtiles des chaînes latérales des monomères de BTA peuvent être utilisées pour modifier de façon rationnelle la structure et les fonctions des homo- et copolymères supramoléculaires. Les propriétés d'assemblage et les fonctions de trois types différents de polymères supramoléculaires à base de monomères BTA ont été étudiées en profondeur. Le premier système

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