Academic literature on the topic 'SLH domain'
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Journal articles on the topic "SLH domain"
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Brechtel, Elke, and Hubert Bahl. "In Thermoanaerobacterium thermosulfurigenes EM1 S-Layer Homology Domains Do Not Attach to Peptidoglycan." Journal of Bacteriology 181, no. 16 (August 15, 1999): 5017–23. http://dx.doi.org/10.1128/jb.181.16.5017-5023.1999.
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ABSTRACT Three exocellular enzymes of Thermoanaerobacterium thermosulfurigenes EM1 possess a C-terminal triplicated sequence related to a domain of bacterial cell surface proteins (S-layer proteins). At least one copy of this sequence, named the SLH (for S-layer homology) domain, is also present at the N terminus of the S-layer protein of this bacterium. The hypothesis that SLH domains serve to anchor proteins to the cell surface was investigated by using the SLH domain-containing xylanase. This enzyme was isolated fromT. thermosulfurigenes EM1, and different forms with and without SLH domains were synthesized in Escherichia coli. The interaction of these proteins with isolated components of the cell envelope was determined to identify the attachment site in the cell wall. In addition, a polypeptide consisting of three SLH domains and the N terminus of the S-layer protein of T. thermosulfurigenes EM1 were included in these studies. The results indicate that SLH domains are necessary for the attachment of these proteins to peptidoglycan-containing sacculi. Extraction of the native sacculi with hydrofluoric acid led to the conclusion that not peptidoglycan but accessory cell wall polymers function as the adhesion component in the cell wall. Our results provide further evidence that attachment of proteins via their SLH domains represents an additional mode to display polypeptides on the cell surfaces of bacteria.
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Ozdemir, Inci, Sara E. Blumer-Schuette, and Robert M. Kelly. "S-Layer Homology Domain Proteins Csac_0678 and Csac_2722 Are Implicated in Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus." Applied and Environmental Microbiology 78, no. 3 (December 2, 2011): 768–77. http://dx.doi.org/10.1128/aem.07031-11.
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ABSTRACTThe genusCaldicellulosiruptorcontains extremely thermophilic bacteria that grow on plant polysaccharides. The genomes ofCaldicellulosiruptorspecies reveal certain surface layer homology (SLH) domain proteins that have distinguishing features, pointing to a role in lignocellulose deconstruction. Two of these proteins inCaldicellulosiruptor saccharolyticus(Csac_0678 and Csac_2722) were examined from this perspective. In addition to three contiguous SLH domains, the Csac_0678 gene encodes a glycoside hydrolase family 5 (GH5) catalytic domain and a family 28 carbohydrate-binding module (CBM); orthologs to Csac_0678 could be identified in all genome-sequencedCaldicellulosiruptorspecies. Recombinant Csac_0678 was optimally active at 75°C and pH 5.0, exhibiting both endoglucanase and xylanase activities. SLH domain removal did not impact Csac_0678 GH activity, but deletion of the CBM28 domain eliminated binding to crystalline cellulose and rendered the enzyme inactive on this substrate. Csac_2722 is the largest open reading frame (ORF) in theC. saccharolyticusgenome (predicted molecular mass of 286,516 kDa) and contains two putative sugar-binding domains, two Big4 domains (bacterial domains with an immunoglobulin [Ig]-like fold), and a cadherin-like (Cd) domain. Recombinant Csac_2722, lacking the SLH and Cd domains, bound to cellulose and had detectable carboxymethylcellulose (CMC) hydrolytic activity. Antibodies directed against Csac_0678 and Csac_2722 confirmed that these proteins bound to theC. saccharolyticusS-layer. Their cellular localization and functional biochemical properties indicate roles for Csac_0678 and Csac_2722 in recruitment and hydrolysis of complex polysaccharides and the deconstruction of lignocellulosic biomass. Furthermore, these results suggest that related SLH domain proteins in otherCaldicellulosiruptorgenomes may also be important contributors to plant biomass utilization.
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Chauvaux, Sylvie, Markus Matuschek, and Pierre Beguin. "Distinct Affinity of Binding Sites for S-Layer Homologous Domains in Clostridium thermocellum and Bacillus anthracis Cell Envelopes." Journal of Bacteriology 181, no. 8 (April 15, 1999): 2455–58. http://dx.doi.org/10.1128/jb.181.8.2455-2458.1999.
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ABSTRACT Binding parameters were determined for the SLH (S-layer homologous) domains from the Clostridium thermocellum outer layer protein OlpB, from the C. thermocellum S-layer protein SlpA, and from the Bacillus anthracis S-layer proteins EA1 and Sap, using cell walls from C. thermocellum and B. anthracis. Each SLH domain bound to C. thermocellumand B. anthracis cell walls with a differentKD , ranging between 7.1 × 10−7 and 1.8 × 10−8 M. Cell wall binding sites for SLH domains displayed different binding specificities in C. thermocellum and B. anthracis. SLH-binding sites were not detected in cell walls of Bacillus subtilis. Cell walls of C. thermocellum lost their affinity for SLH domains after treatment with 48% hydrofluoric acid but not after treatment with formamide or dilute acid. A soluble component, extracted from C. thermocellum cells by sodium dodecyl sulfate treatment, bound the SLH domains from C. thermocellum but not those from B. anthracisproteins. A corresponding component was not found in B. anthracis.
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Kosugi, Akihiko, Koichiro Murashima, Yutaka Tamaru, and Roy H. Doi. "Cell-Surface-Anchoring Role of N-Terminal Surface Layer Homology Domains of Clostridium cellulovorans EngE." Journal of Bacteriology 184, no. 4 (February 15, 2002): 884–88. http://dx.doi.org/10.1128/jb.184.4.884-888.2002.
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ABSTRACT engE, coding for endoglucanase E, one of the three major subunits of the Clostridium cellulovorans cellulosome, has been cloned and sequenced (Y. Tamaru and R. H. Doi, J. Bacteriol. 181:3270-3276, 1999). The N-terminal-half region of EngE possesses three repeated surface layer homology (SLH) domains, which are homologous to those of some bacterial S-layer proteins. Also, the C-terminal-half region consists of a catalytic domain of glycosyl hydrolase family 5 and a duplicated sequence (dockerin) for binding EngE to scaffolding protein CbpA. Our hypothesis is that the SLH domains serve in the role of anchoring to the cell surface. This model was investigated by using recombinant EngEs (rEngE) with and without SLH domains that were synthesized in Escherichia coli and cell wall preparations from C. cellulovorans. When rEngE and SLH polypeptides of EngE were incubated with cell wall fragments prepared by sodium dodecyl sulfate treatment, these proteins bound strongly to the cell wall. However, rEngEs without SLH domains lost their ability to bind to cell walls. When rEngE was incubated with mini-CbpA, consisting of two cohesin domains, and cell wall fragments, the mini-CbpA was able to bind to the cell wall with rEngE. However, the binding of mini-CbpA was dramatically inhibited by addition of a chelating reagent, such as EDTA, which prevents cohesin-dockerin interactions. These results suggest not only that the SLH domains of EngE can bind to the cell surface but also that EngE plays an anchoring role for cellulosomes through the interaction of its dockerin domain with a CbpA cohesin.
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Cann, Isaac K. O., Svetlana Kocherginskaya, Michael R. King, Bryan A. White, and Roderick I. Mackie. "Molecular Cloning, Sequencing, and Expression of a Novel Multidomain Mannanase Gene from Thermoanaerobacterium polysaccharolyticum." Journal of Bacteriology 181, no. 5 (March 1, 1999): 1643–51. http://dx.doi.org/10.1128/jb.181.5.1643-1651.1999.
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ABSTRACT The manA gene of Thermoanaerobacterium polysaccharolyticum was cloned in Escherichia coli. The open reading frame of manA is composed of 3,291 bases and codes for a preprotein of 1,097 amino acids with an estimated molecular mass of 119,627 Da. The start codon is preceded by a strong putative ribosome binding site (TAAGGCGGTG) and a putative −35 (TTCGC) and −10 (TAAAAT) promoter sequence. The ManA of T. polysaccharolyticum is a modular protein. Sequence comparison and biochemical analyses demonstrate the presence of an N-terminal leader peptide, and three other domains in the following order: a putative mannanase-cellulase catalytic domain, cellulose binding domains 1 (CBD1) and CBD2, and a surface-layer-like protein region (SLH-1, SLH-2, and SLH-3). The CBD domains show no sequence homology to any cellulose binding domain yet reported, hence suggesting a novel CBD. The duplicated CBDs, which lack a disulfide bridge, exhibit 69% identity, and their deletion resulted in both failure to bind to cellulose and an apparent loss of carboxymethyl cellulase and mannanase activities. At the C-terminal region of the gene are three repeats of 59, 67, and 56 amino acids which are homologous to conserved sequences found in the S-layer-associated regions within the xylanases and cellulases of thermophilic members of theBacillus-Clostridium cluster. The ManA of T. polysaccharolyticum, besides being an extremely active enzyme, is the only mannanase gene cloned which shows this domain structure.
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Li, Jia, Xiaomin Hu, Jianpin Yan, and Zhiming Yuan. "Species-Specific Cell Wall Binding Affinity of the S-Layer Proteins of Mosquitocidal Bacterium Bacillus sphaericus C3-41." Applied and Environmental Microbiology 75, no. 12 (April 24, 2009): 3891–95. http://dx.doi.org/10.1128/aem.00356-09.
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ABSTRACT The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring the S-layer subunits to the rigid cell wall layer via a distinct type of secondary cell wall polymer and that a motif of the recombinant SLH polypeptide comprising amino acids 152 to 210 (rSLH152-210) was essential for the stable binding of the S-layer with the bacterial cell walls. The quantitative assays revealed that the KD (equilibrium dissociation constant) values of rSLH152-210 and rSLH31-210 with purified cell wall sacculi were 1.11 × 10−6 M and 1.40 × 10−6 M, respectively. The qualitative assays demonstrated that the SLH domain of strain C3-41 could bind only to the cell walls or the cells treated with 5 M guanidinium hydrochloride of both toxic and nontoxic B. sphaericus strains but not to those from other bacteria, indicating the species-specific binding of the SLH polypeptide of B. sphaericus with bacterial cell walls.
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Kojima, Seiji, Kyong-Cheol Ko, Yumiko Takatsuka, Naoki Abe, Jun Kaneko, Yoshifumi Itoh, and Yoshiyuki Kamio. "Cadaverine Covalently Linked to Peptidoglycan Is Required for Interaction between the Peptidoglycan and the Periplasm-Exposed S-Layer-Homologous Domain of Major Outer Membrane Protein Mep45 in Selenomonas ruminantium." Journal of Bacteriology 192, no. 22 (September 17, 2010): 5953–61. http://dx.doi.org/10.1128/jb.00417-10.
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ABSTRACT The peptidoglycan of Selenomonas ruminantium is covalently bound to cadaverine (PG-cadaverine), which likely plays a significant role in maintaining the integrity of the cell surface structure. The outer membrane of this bacterium contains a 45-kDa major protein (Mep45) that is a putative peptidoglycan-associated protein. In this report, we determined the nucleotide sequence of the mep45 gene and investigated the relationship between PG-cadaverine, Mep45, and the cell surface structure. Amino acid sequence analysis showed that Mep45 is comprised of an N-terminal S-layer-homologous (SLH) domain followed by α-helical coiled-coil region and a C-terminal β-strand-rich region. The N-terminal SLH domain was found to be protruding into the periplasmic space and was responsible for binding to peptidoglycan. It was determined that Mep45 binds to the peptidoglycan in a manner dependent on the presence of PG-cadaverine. Electron microscopy revealed that defective PG-cadaverine decreased the structural interactions between peptidoglycan and the outer membrane, consistent with the proposed role for PG-cadaverine. The C-terminal β-strand-rich region of Mep45 was predicted to be a membrane-bound unit of the 14-stranded β-barrel structure. Here we propose that PG-cadaverine possesses functional importance to facilitate the structural linkage between peptidoglycan and the outer membrane via specific interaction with the SLH domain of Mep45.
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Tamaru, Yutaka, and Roy H. Doi. "Three Surface Layer Homology Domains at the N Terminus of the Clostridium cellulovorans Major Cellulosomal Subunit EngE." Journal of Bacteriology 181, no. 10 (May 15, 1999): 3270–76. http://dx.doi.org/10.1128/jb.181.10.3270-3276.1999.
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ABSTRACT The gene engE, coding for endoglucanase E, one of the three major subunits of the Clostridium cellulovoranscellulosome, has been isolated and sequenced. engE is comprised of an open reading frame (ORF) of 3,090 bp and encodes a protein of 1,030 amino acids with a molecular weight of 111,796. The amino acid sequence derived from engE revealed a structure consisting of catalytic and noncatalytic domains. The N-terminal-half region of EngE consisted of a signal peptide of 31 amino acid residues and three repeated surface layer homology (SLH) domains, which were highly conserved and homologous to an S-layer protein from the gram-negative bacterium Caulobacter crescentus. The C-terminal-half region, which is necessary for the enzymatic function of EngE and for binding of EngE to the scaffolding protein CbpA, consisted of a catalytic domain homologous to that of family 5 of the glycosyl hydrolases, a domain of unknown function, and a duplicated sequence (DS or dockerin) at its C terminus. engE is located downstream of an ORF, ORF1, that is homologous to theBacillus subtilis phosphomethylpyrimidine kinase (pmk) gene. The unique presence of three SLH domains and a DS suggests that EngE is capable of binding both to CbpA to form a CbpA-EngE cellulosome complex and to the surface layer of C. cellulovorans.
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Lupas, Andrei. "A circular permutation event in the evolution of the SLH domain?" Molecular Microbiology 20, no. 4 (May 1996): 897–98. http://dx.doi.org/10.1111/j.1365-2958.1996.tb02528.x.
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Fukuda, Mutsumi, Seiji Watanabe, Shigeki Yoshida, Hiroya Itoh, Yoshifumi Itoh, Yoshiyuki Kamio, and Jun Kaneko. "Cell Surface Xylanases of the Glycoside Hydrolase Family 10 Are Essential for Xylan Utilization by Paenibacillus sp. W-61 as Generators of Xylo-Oligosaccharide Inducers for the Xylanase Genes." Journal of Bacteriology 192, no. 8 (February 12, 2010): 2210–19. http://dx.doi.org/10.1128/jb.01406-09.
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ABSTRACT Paenibacillus sp. W-61 is capable of utilizing water-insoluble xylan for carbon and energy sources and has three xylanase genes, xyn1, xyn3, and xyn5. Xyn1, Xyn3, and Xyn5 are extracellular enzymes of the glycoside hydrolase (GH) families 11, 30, and 10, respectively. Xyn5 contains several domains including those of carbohydrate-binding modules (CBMs) similar to a surface-layer homologous (SLH) protein. This study focused on the role of Xyn5, localized on the cell surface, in water-insoluble xylan utilization. Electron microscopy using immunogold staining revealed Xyn5 clusters over the entire cell surface. Xyn5 was bound to cell wall fractions through its SLH domain. A Δxyn5 mutant grew poorly and produced minimal amounts of Xyn1 and Xyn3 on water-insoluble xylan. A Xyn5 mutant lacking the SLH domain (Xyn5ΔSLH) grew poorly, secreting Xyn5ΔSLH into the medium and producing minimal Xyn1 and Xyn3 on water-insoluble xylan. A mutant with an intact xyn5 produced Xyn5 on the cell surface, grew normally, and actively synthesized Xyn1 and Xyn3 on water-insoluble xylan. Quantitative reverse transcription-PCR showed that xylobiose, generated from water-insoluble xylan decomposition by Xyn5, is the most active inducer for xyn1 and xyn3. Luciferase assays using a Xyn5-luciferase fusion protein suggested that xylotriose is the best inducer for xyn5. The cell surface Xyn5 appears to play two essential roles in water-insoluble xylan utilization: (i) generation of the xylo-oligosaccharide inducers of all the xyn genes from water-insoluble xylan and (ii) attachment of the cells to the substrate so that the generated inducers can be immediately taken up by cells to activate expression of the xyn system.
Dissertations / Theses on the topic "SLH domain"
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Ryzhkov, Pavel. "Bioengineering of S-layers: molecular characterization of the novel S-layer gene sslA of Sporosarcina ureae ATCC 13881 and nanotechnology application of SslA protein derivatives." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1204126129404-82781.
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S-layer proteins of S. ureae ATCC 13881 form on the cell surface an S-layer lattice with p4 square type symmetry and a period of about 13.5 nm. These lattices were shown to be the excellent nanotemplates for deposition of regular metal clusters. The synthesis of the S. ureae S-layer protein is highly efficient, the protein accounts for approximately 10-15 % of the total cell protein content, judged by the SDS-PAGE results. Besides, the S-layer protein production is tightly regulated, since only negligible amounts of S-layer proteins are observed in the medium at different cell growth phases. At the same time, mechanisms of the regulation of S-layer protein synthesis are poorly understood. As several hundreds of S-layer proteins are produced per second during the cell growth, the S-layer gene promoters are among the strongest prokaryotic promoters at all. However, little is known about factors regulating the expression of S-layer genes, furthermore, no experimental identification of other upstream regulatory sequences except for -35/-10 and RBS sequences was presented to our knowledge to date. A sequence of the S-layer gene of S. ureae ATCC 13881, encoding the previously described S-layer protein, was identified in this work by combination of different approaches. The largest part of the gene, excluding its upstream regulatory and ORF 5’ regions, was isolated from a genomic library by hybridization. The sequence of the isolated fragment proved to contain additionally an 1.9 kb non-coding region and an incomplete 0.8 kb ORF region in its 3’-part. No RBS sequence and apparent promoter regions could be identified in front of the latter sequence, suggesting that it might represent a pseudogene sequence. The sequences of the 5’ and upstream regions of the S. ureae ATCC 13881 S-layer gene were identified by combination of PCR-sequencing and chromosome walking. Totally, a sequence of the 6.4 kb long region of S. ureae genomic DNA was established. The sequence of the S. ureae S-layer protein was deduced from the respective gene sequence and agreed with the peptide sequences, obtained after N-terminal sequencing of tryptic peptides of the S. ureae ATCC 13881 S-layer protein. For the protein the name SslA was proposed, which is an abbreviation for “Sporosarcina ureae S-layer protein A”. Several specific features were observed in gene organisation of sslA, which are also characteristic for other S-layer genes. The distance between the -35/-10 region and the ATG initiation codon is unusually long and a 41 bp palindromic sequence is present in the immediate vicinity of the -35/-10 region. Besides, a distant location of the rho-independent transcription terminator, which is 647 bp remote from the stop codon, will result in the mRNA transcripts with unusually long trailer region. Both the long 5’ UTR and the long 3’ trailer may have a regulatory function, either by conferring increased mRNA stability and/or by affecting translation efficiency. Potentially these sequences may define the binding sites of regulatory proteins. For example, palindromic sequences constitute the regulatory sites in several bacterial operons and may act as the binding sites of regulatory dimeric proteins. In respect to the conservation of the sslA sequence high similarity to the sequences of other functional S-layer genes, especially the slfA and slfB genes of B. sphaericus, was observed, whereas the results of phylogenetic analysis support the hypothesis that S-layer genes may have evolved via the lateral gene transfer. Based on the sslA sequence, several recombinant proteins with truncations of the terminal protein parts or C-terminal fusion of either EGFP or histidine tags were constructed. For all the truncated or EGFP-fusion SslA derivatives high level overexpression in E. coli was possible. For native SslA a moderate level of expression was observed suggesting that its high intracellular concentration may downregulate the protein synthesis. Interestingly, fluorescence microscopy indicates the same intracellular localization for heterologously produced recombinant proteins with fusions of EGFP either to the precursor or to the native SslA protein, suggesting that SslA secretion signal is not functional in E. coli. Heterologously produced SslA derivatives with truncations of N-, C- or both N- and C-terminal parts were shown to self- assemble in vitro, although the size of self-assembly structures was different from that observed upon the self-assembly of the native SslA. In the latter case extended self-assembly layers with the size up to 5x10 µm were observed, with a surface area of up to two orders of magnitude higher than that of S-layer patches, routinely isolated from S. ureae surface. Dependent on the applied recrystallization conditions preferential formation of single- or multilayer self-assembly structures was observed.
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Ryzhkov, Pavel. "Bioengineering of S-layers: molecular characterization of the novel S-layer gene sslA of Sporosarcina ureae ATCC 13881 and nanotechnology application of SslA protein derivatives." Doctoral thesis, Technische Universität Dresden, 2007. https://tud.qucosa.de/id/qucosa%3A24121.
Full textAbstract:
S-layer proteins of S. ureae ATCC 13881 form on the cell surface an S-layer lattice with p4 square type symmetry and a period of about 13.5 nm. These lattices were shown to be the excellent nanotemplates for deposition of regular metal clusters. The synthesis of the S. ureae S-layer protein is highly efficient, the protein accounts for approximately 10-15 % of the total cell protein content, judged by the SDS-PAGE results. Besides, the S-layer protein production is tightly regulated, since only negligible amounts of S-layer proteins are observed in the medium at different cell growth phases. At the same time, mechanisms of the regulation of S-layer protein synthesis are poorly understood. As several hundreds of S-layer proteins are produced per second during the cell growth, the S-layer gene promoters are among the strongest prokaryotic promoters at all. However, little is known about factors regulating the expression of S-layer genes, furthermore, no experimental identification of other upstream regulatory sequences except for -35/-10 and RBS sequences was presented to our knowledge to date. A sequence of the S-layer gene of S. ureae ATCC 13881, encoding the previously described S-layer protein, was identified in this work by combination of different approaches. The largest part of the gene, excluding its upstream regulatory and ORF 5’ regions, was isolated from a genomic library by hybridization. The sequence of the isolated fragment proved to contain additionally an 1.9 kb non-coding region and an incomplete 0.8 kb ORF region in its 3’-part. No RBS sequence and apparent promoter regions could be identified in front of the latter sequence, suggesting that it might represent a pseudogene sequence. The sequences of the 5’ and upstream regions of the S. ureae ATCC 13881 S-layer gene were identified by combination of PCR-sequencing and chromosome walking. Totally, a sequence of the 6.4 kb long region of S. ureae genomic DNA was established. The sequence of the S. ureae S-layer protein was deduced from the respective gene sequence and agreed with the peptide sequences, obtained after N-terminal sequencing of tryptic peptides of the S. ureae ATCC 13881 S-layer protein. For the protein the name SslA was proposed, which is an abbreviation for “Sporosarcina ureae S-layer protein A”. Several specific features were observed in gene organisation of sslA, which are also characteristic for other S-layer genes. The distance between the -35/-10 region and the ATG initiation codon is unusually long and a 41 bp palindromic sequence is present in the immediate vicinity of the -35/-10 region. Besides, a distant location of the rho-independent transcription terminator, which is 647 bp remote from the stop codon, will result in the mRNA transcripts with unusually long trailer region. Both the long 5’ UTR and the long 3’ trailer may have a regulatory function, either by conferring increased mRNA stability and/or by affecting translation efficiency. Potentially these sequences may define the binding sites of regulatory proteins. For example, palindromic sequences constitute the regulatory sites in several bacterial operons and may act as the binding sites of regulatory dimeric proteins. In respect to the conservation of the sslA sequence high similarity to the sequences of other functional S-layer genes, especially the slfA and slfB genes of B. sphaericus, was observed, whereas the results of phylogenetic analysis support the hypothesis that S-layer genes may have evolved via the lateral gene transfer. Based on the sslA sequence, several recombinant proteins with truncations of the terminal protein parts or C-terminal fusion of either EGFP or histidine tags were constructed. For all the truncated or EGFP-fusion SslA derivatives high level overexpression in E. coli was possible. For native SslA a moderate level of expression was observed suggesting that its high intracellular concentration may downregulate the protein synthesis. Interestingly, fluorescence microscopy indicates the same intracellular localization for heterologously produced recombinant proteins with fusions of EGFP either to the precursor or to the native SslA protein, suggesting that SslA secretion signal is not functional in E. coli. Heterologously produced SslA derivatives with truncations of N-, C- or both N- and C-terminal parts were shown to self- assemble in vitro, although the size of self-assembly structures was different from that observed upon the self-assembly of the native SslA. In the latter case extended self-assembly layers with the size up to 5x10 µm were observed, with a surface area of up to two orders of magnitude higher than that of S-layer patches, routinely isolated from S. ureae surface. Dependent on the applied recrystallization conditions preferential formation of single- or multilayer self-assembly structures was observed.
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García, García Andrés. "SLA-Driven Cloud Computing Domain Representation and Management." Doctoral thesis, Universitat Politècnica de València, 2014. http://hdl.handle.net/10251/36579.
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The assurance of Quality of Service (QoS) to the applications, although identified as a key feature since long ago [1], is one of the fundamental challenges that remain unsolved. In the Cloud Computing context, Quality of Service is defined as the measure of the compliance of certain user requirement in the delivery of a cloud resource, such as CPU or memory load for a virtual machine, or more abstract and higher level concepts such as response time or availability. Several research groups, both from academia and industry, have started working on describing the QoS levels that define the conditions under which the service need to be delivered, as well as on developing the necessary means to effectively manage and evaluate the state of these conditions.
[2] propose Service Level Agreements (SLAs) as the vehicle for the definition of QoS guarantees, and the provision and management of resources. A Service Level Agreement (SLA) is a formal contract between providers and consumers, which defines the quality of service, the obligations and the guarantees in the delivery of a specific good. In the context of Cloud computing, SLAs are considered to be machine readable documents, which are automatically managed by the provider's platform.
SLAs need to be dynamically adapted to the variable conditions of resources and applications. In a multilayer architecture, different parts of an SLA may refer to different resources. SLAs may therefore express complex relationship between entities in a changing environment, and be applied to resource selection to implement intelligent scheduling algorithms.
Therefore SLAs are widely regarded as a key feature for the future development of Cloud platforms. However, the application of SLAs for Grid and Cloud systems has many open research lines. One of these challenges, the modeling of the landscape, lies at the core of the objectives of the Ph. D. Thesis.García García, A. (2014). SLA-Driven Cloud Computing Domain Representation and Management [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/36579
TESIS
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Koller, Bastian [Verfasser]. "Enhanced SLA management in the high performance computing domain / vorgelegt von Bastian Koller." Stuttgart : HLRS, 2011. http://d-nb.info/1011191083/34.
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Wong, Iris Yuen Ting. "An analysis of domain name dispute resolution in Hong Kong." access abstract and table of contents access full-text, 2005. http://libweb.cityu.edu.hk/cgi-bin/ezdb/dissert.pl?ma-slw-b20835863a.pdf.
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Thesis (M.A.)--City University of Hong Kong, 2005.Title from title screen (viewed on 27 Mar. 2006) "Master of arts in arbitration and dispute resolution research paper." Includes bibliographical references.
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Kandalaft, Hiba. "Isolation and Characterization of Anti-SLP Single Domain Antibodies for the Therapy of C. difficile Infection." Thesis, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/20624.
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Clostridium difficile is the leading cause of death from gastrointestinal infections in Canada. Current antiobiotic treatment is non-ideal due to the high incidence of relapse and the rise in hyper-virulent antibiotic-resistant strains. Surface layer proteins (SLPs) cover the entire bacterial surface and mediate adherence to host cells. Passive and active immunization against SLPs greatly enhances survival in hamsters, suggesting that antibody-mediated bacterial neutralization may be an effective alternative therapeutic strategy. Using a recombinant-antibody phage display library, and SLPs from strain QCD 32g58 as bait antigen, we isolated and extensively characterized 11 SLP-specific recombinant single-domain antibodies (sdAbs), in terms of affinity and specificity, intrinsic stability, and ability to inhibit cell motility. Several sdAbs exhibit promising characteristics for a potential oral therapeutic based on their high affinity, high thermal stability, and resistance to pepsin digestion. Our study provides the basis of a proof-of-principle model with which to develop specific, broadly neutralizing and intrinsically stable antibodies for the oral therapy of C. difficile infections, as an alternative to conventional antibiotic treatment.
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Yang, Jun. "A Smoothed Dissipative Particle Dynamics Methodology For Wall-Bounded Domains." Digital WPI, 2013. https://digitalcommons.wpi.edu/etd-dissertations/225.
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This work presents the mathematical and computational aspects of a smooth dissipative particle dynamics with dynamic virtual particle allocation method (SDPD-DV) for modeling and simulation of mesoscopic fluids in wall-bounded domains. The SDPD-DV method is realized with fluid particles, boundary particles and dynamically allocated virtual particles near solid boundaries. The physical domain in SDPD-DV contains external and internal solid boundaries, periodic inlets and outlets, and the fluid region. The solid boundaries of the domain are represented with boundary particles which have an assigned position, wall velocity, and temperature upon initialization. The fluid domain is discretized with fluid particles placed in a global index. The algorithm for nearest neighbor particle search is based on a combination of the linked-cell and Verlet-list approaches and utilizes large rectangular cells for computational efficiency. The density model of a fluid particle in the proximity of a solid boundary includes the contribution from the virtual particles in its truncated support domain. The thermodynamic properties of a virtual particle are identical to those of the corresponding fluid particle. A periodic boundary particle allocation method is used at periodic inlets and outlets. Models for the conservative and dissipative forces on a fluid particle in the proximity of a solid boundary are presented and include the contributions of the virtual particles in its truncated support domain. The integration of the fluid particle position and momentum equations is accomplished with an implementation of the velocity-Verlet algorithm. The integration is supplemented by a bounce-forward algorithm in cases where the virtual particle force model is not able to prevent particle penetration. The integration of the entropy equation is based on the Runge-Kutta scheme. In isothermal simulations, the pressure of a fluid particle is obtained by an artificial compressibility formulation for liquids and the ideal gas law for compressible fluids. Sampling methods used for particle properties and transport coefficients in SDPD-DV are presented. The self-diffusion coefficient is obtained by an implementation of the generalized Einstein and the Green-Kubo relations. Field properties are obtained by sampling SDPD-DV outputs on a post-processing grid that allows harnessing the particle information on desired spatio-temporal scales. The isothermal (without the entropy equation) SDPD-DV method is verified and validated with simulations in bounded and periodic domains that cover the hydrodynamic and mesoscopic regimes. Verification is achieved with SDPD-DV simulations of transient, Poiseuille, body-force driven flow of liquid water between plates separated. The velocity profiles from the SDPD-DV simulations are in very good agreement with analytical estimates and the field density fluctuation near solid boundaries is shown to be below 5%. Additional verification involves SDPD-DV simulations of transient, planar, Couette liquid water flow. The top plate is moving and separated from the bottom stationary plate. The numerical results are in very good agreement with the analytical solutions. Additional SDPD-DV verification is accomplished with the simulation of a body-force driven, low-Reynolds number flow of water over a cylinder of radius R=0.02m. The SDPD-DV field velocity and pressure are compared with those obtained by FLUENT. An extensive set of SDPD-DV simulations of liquid water and gaseous nitrogen in mesoscopic periodic domains is presented. For the SDPD-DV simulations of liquid water the mass of the fluid particles is varied between 1.24 and 3.3e-7 real molecular masses and their corresponding size is between 1.08 and 323 physical length scales. For SDPD-DV simulations of gaseous nitrogen the mass of the fluid particles is varied between 6.37e3and 6.37e6 real molecular masses and their corresponding size is between 2.2e2 and 2.2e3 physical length scales. The equilibrium states are obtained and show that the particle speeds scale inversely with particle mass (or size) and that the translational temperature is scale-free. The self-diffusion coefficient for liquid water is obtained through the mean-square displacement and the velocity auto-correlation methods for the range of fluid particle masses (or sizes) considered. Various analytical expressions for the self-diffusivity of the SDPD fluid are developed in analogy to the real fluid. The numerical results are in very good agreement with the SDPD-fluid analytical expressions. The numerical self-diffusivity is shown to be scale dependent. For fluid particles approaching asymptotically the mass of the real particle the self-diffusivity is shown to approach the experimental value. The Schmidt numbers obtained from the SDPD-DV simulations are within the range expected for liquid water. The SDPD-DV method (with entropy) is verified and validated with simulations with an extensive set of simulations of gaseous nitrogen in mesoscopic, periodic domains in equilibrium. The simulations of N2(g) are performed in rectangular domains. The self-diffusion coefficient for N2(g) at equilibrium states is obtained through the mean-square displacement for the range of fluid particle masses (or sizes) considered. The numerical self-diffusion is shown to be scale dependent. The simulations show that self-diffusion decreases with increasing mass ratio. For a given mass ratio, increasing the smoothing length, increases the self-diffusion coefficient. The shear viscosity obtained from SDPD-DV is shown to be scale free and in good agreement with the real value. We examine also the effects of timestep in SDPD-DV simulations by examining thermodynamic parameters at equilibrium. These results show that the time step can lead to a significant error depending on the fluid particle mass and smoothing length. Fluctuations in thermodynamic variables obtained from SDPD-DV are compared with analytical estimates. Additional verification involves SDPD-DV simulations of steady planar thermal Couette flow of N2(g). The top plate at temperature T1 =330K is moving at Vxw =30m/s and is separated by 10-4 m from the bottom stationary plate at T2=300K. The SDPD-DV velocity and temperature fields are in excellent agreement with those obtained by FLUENT.
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Leduc, Julien. "Etude physique et numérique de l'écoulement dans un dispositif d'injection de turbine Pelton." Phd thesis, Ecole Centrale de Lyon, 2010. http://tel.archives-ouvertes.fr/tel-00684385.
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La turbine Pelton est une turbine hydraulique dont le fonctionnement se caractérise par l'interaction d'un jet d'eau avec les augets d'une roue. Cette étude a pour but de comprendre les phénomènes influençant le jet et son interaction avec les augets. Pour cela deux actions différentes ont été menées. Une première a visé à caractériser expérimentalement la fragmentation d'un jet de turbine Pelton. La seconde s'est attachée à développer une méthode numérique pouvant mener'à la simulation précise de jets réels de turbines Pelton. La partie expérimentale a permis de déterminer le mode de fragmentation de ces jets (atomisation turbulente), mais aussi l'influence de la rugosité des parois de l'injecteur sur les performances de la turbine. La participation de ce travail à un projet expérimental a permis de montrer l'influence de l'écoulement en sortie d'injecteur sur la fragmentation du jet. Les phénomènes physiques influençant principalement l'évolution du jet ont ainsi été déterminés. La partie numérique a eu pour but de mettre en place une méthode permettant de simuler l'évolution d'un jet de turbine Pelton (fragmentation) et son interaction avec un auget. Etant donnés les progrès de la méthode SPH-ALE pour la simulation d'impact de jets pour les turbines Pelton, il a été décidé d'adapter cette méthode pour les simulations visées. Ainsi une étude du choix de la vitesse des interfaces de problème de Riemann a permis de réaliser un modèle multiphase stable pour les forts rapports de densité (eau-air). Cette méthode s'est avérée garantir les propriétés de continuité de vitesse normale et de pression à l'interface entre les fluides. L'ajout des phénomènes de tension de surface s'est fait par l'adaptation du modèle CSF (Continuum Surface Force) et le développement d'un second modèle nommé Laplace Law Pressure Correction (LLPC).L'intégration du saut de pression dans le solveur de Riemann a nécessité une étude précise du calcul de la courbure et a permis d'améliorer la simulation de loi de Laplace. La méthode numérique a été ensuite validée sur les cas académiques d'onde gravitaire, de rupture de barrage et d'oscillation de goutte. Les ressources en mémoire et le temps de calcul associé à cette méthode ont nécessité la parallélisation du code de calcul. Le caractère lagrangien de la méthode a très largement influencé la méthode de découpe de domaine pour permettre une bonne répartition de la charge de calcul entre les différents processeurs. En conclusion les phénomènes physiques influençant la fragmentation de jets issus d'injecteurs de turbine Pelton sont désormais mieux connus et ils ont pu être introduits dans la méthode numérique. Les prochains développements porteront sur la simulation de jets dont la condition d'entrée s'attachera à être représentative des caractéristiques d'un écoulement en sortie d'un injecteur de turbine Pelton.
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Lamali, Mohamed Lamine. "Qualité de service et calcul de chemins dans les réseaux inter-domaine et multicouches." Thesis, Versailles-St Quentin en Yvelines, 2014. http://www.theses.fr/2014VERS0046/document.
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La Qualité de Service (Quality of Service - QoS) est une garantie de paramètres réseau (bande passante élevée, délai court, etc.). Dans un réseaux inter-domaine, elle peut être assurée par des contrats entre domaines appelés Service Level Agreements (SLA). Dans cette thèse, nous nous intéressons d’abord à l’étape de négociation de SLA : la sélection des SLA proposés par un domaine. Nous proposons des méthodes exactes et approchées permettant aux domaines de proposer les SLA qui maximisent leurs revenus. Nous étudions également l'impact de la réputation des domaines sur cette négociation. Au niveau de l’instanciation des SLA, nous nous intéressons au calcul de chemins qui prennent en compte les encapsulations de protocoles (afin de pallier l’hétérogénéité technologique des domaines). En utilisant des outils de théorie des langages, nous proposons la première solution polynomiale au calcul de chemins dans un tel contexteQuality of Service (QoS) is a guarantee of network parameters (high bandwidth, short delay, etc.). In an inter-domaine network, it can be provided by contracts between domains, called Service Level Agreements (SLAs). In this thesis, we first focus on the SLA negotiation step, i.e., the selection of SLAs proposed by a domain. We provide exact and approximate methods that allow the domains to propose the SLAs that maximize their revenues. We also study the impact of the domain reputation on the negotiation process. Regarding the SLA instantiation step, we focus on path computation processes that take into account the encapsulation of protocols (in order to overcome the domain technological heterogeneity). Using tools from Language Theory, we provide the first polynomial solution to the path computation problem in this context
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Ivanova, Svilena. "Glycoprotéines d'enveloppes (Env) des gamma- et delta-rétrovirus et leurs récepteurs : recherche chez les mammifères de nouveaux récepteurs d'Env associés au métabolisme cellulaire et d'Env endogènes apparentées." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTT052.
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Couverture)Les rétrovirus sont des virus enveloppés à ARN simple brin omniprésents dans le monde animal et sources de nombreuses pathologies. Les rétrovirus de vertébrés comprennent sept genres dont les gamma et deltarétrovirus qui sont l’objet de ces travaux. Les rétrovirus dits endogènes (ERV), par opposition à leurs homologues infectieux exogènes, sont présents dans les cellules germinales et font partie intégrante du patrimoine génétique, avec transmission mendélienne. Au cours de l'évolution, les ERV ont fait l'objet de mutations, rendant défectives la plupart des copies dans les génomes de vertébrés, avec quelques exceptions notoires. De fait, certaines copies maintiennent de larges cadres de lecture suite à une pression de sélection positive.Rétrovirus exogènes et ERV partagent une organisation génétique similaire. Leurs glycoprotéines d’enveloppe (Env), dont une des propriétés est de lier un récepteur cellulaire, comprennent une composante de surface (SU) associée à une partie transmembranaire (TM). La SU des Env γ et -rétrovirales porte un module RBD (Receptor-Binding Domain) qui lie un récepteur appartenant à la famille SLC (Solute Carriers) des transporteurs de nutriments. Les SLC présents à la surface cellulaire conditionnent le métabolisme des cellules. Afin de pallier l'absence d'anticorps fiables reconnaissant les parties extracellulaires (exofaciales) des SLC, le laboratoire a dérivé des RBD solubles comme ligands des SLC, permettant de suivre leur expression à la surface cellulaire et ainsi, évaluer le métabolisme cellulaire.Parmi les ERV, certaines env partiellement ou entièrement conservées jouent un rôle physiologique essentiel dans les organismes qui les portent. Une hypothèse de mon laboratoire d’accueil est l’existence de RBD endogènes de mammifères capables de moduler le métabolisme cellulaire de leurs hôtes. Dans ce contexte, mes travaux sont articulés autour de deux axes : (i) identifier et produire de nouveaux RBD dérivés des ERV et (ii) identifier de nouveaux transporteurs de type SLC reconnus par des RBD issus de rétrovirus exogènes et ERV de mammifères. Nous avons identifié et caractérisé deux nouveaux RBD humains endogènes (HERV-41 et HERV-89), entrés et conservés chez les primates de l’Ancien Monde il y a environ 35 millions d’années. Nous avons caractérisé leurs séquences PBS (Primer Binding Site), amorces putatives de la réplication rétrovirale, comme étant complémentaires de l’ARNtLeu ou ARNtArg pour HERV-89, et de l'ARNtGlu pour HERV-41. Les séquences env les plus proches dans le génome humain présentent respectivement 38% et 69% d'identité, indiquant l'appartenance de HERV-89 à deux nouvelles familles d'Env. Nous avons pu produire le RBD soluble de HERV-89, montrer que son récepteur est distinct de l'Env HERV ayant la séquence la plus homologue, et étudier sa distribution tissulaire. Le RBD HERV-89 lie un récepteur sur de nombreuses cellules souches et lignées cellulaires établies et nous avons montré par immunohistochimie que le récepteur est exprimé de manière différentielle dans les tissus humains sains et tumoraux. Parallèlement, nous avons dérivé une banque d'expression de 170 SLC que nous avons utilisée pour le criblage à haut-débit de récepteurs des Env gamma et deltarétrovirales. Cette banque nous a permis d'identifier le récepteur, longtemps recherché, de l’Env du virus de la leucémie bovine (BLV). De plus, en utilisant la transfection d'une banque d’expression d’ADNc dans des cellules de hamster, nous avons aussi identifié le récepteur du virus endogène félin ERV-DC14/FeLV-D comme étant le transporteur de cuivre et de cisplatine CTR1/SLC31A1.L’identification du récepteur de BLV pourrait notamment aider dans la lutte contre la transmission du virus et les pathologies associées qui affectent environ 5% du bétail infecté. De plus, les BLV-RBD et DC14-RBD constituent respectivement de nouveaux marqueurs et modulateurs potentiels du métabolisme, dont celui du cuivreRetroviruses are enveloped, single-stranded RNA viruses, that are omnipresent in animals and the causal agents of a large array of pathologies. Vertebrate retroviruses are divided into seven genera, including the γ and -retroviral groups, which we study particularly. Endogenous retroviruses (ERV), as opposed to exogenous infectious viruses, are present in germline cells and as such are bona fide components of the host genome, with Mendelian transmission. Most ERV have been inactivated by purifying mutations during evolution, although a few copies have been subjected to positive selection pressure with conserved open reading frames (ORFs).Exogenous viruses and ERV that belong to gamma and deltaretroviruses share similar genetic organization and their envelope glycoproteins (Env) comprises a transmembrane (TM) and a surface (SU) component, which binds a specific receptor on the host cell membrane. The SU contains a receptor-binding domain (RBD), responsible for receptor recognition, while TM engages membrane fusion and harbors an immunosuppressive domain. Noticeably, some ERVs have maintained entire or partial ORFs in env, which have been shown, in certain cases, to have essential physiological functions.Another common feature of gamma and deltaretroviral Env is the nature of their receptors, which, when identified, all belong to the solute carrier family of nutrient transporters (SLCs). The laboratory derived soluble RBDs from complete Env that can bind cognate receptors and be used to monitor SLC receptor expression at the cell surface. This important property of RBDs overcomes the notorious lack of reliable anti-SLC exofacial antibodies and provides a new way to evaluate, or even modulate, cell metabolism.Our laboratory postulates that some endogenous RBD-coding genes have been positively selected in their hosts for properties linked to binding SLCs and modulating host cell metabolism. In this context, the aim of my work was to: (i) search for new natural endogenous RBDs and (ii) characterize SLC transporters recognized by RBDs derived from ERVs or exogenous infectious mammalian retroviruses.Here, we describe the identification of two novel human endogenous RBDs (HERV-41 and HERV-89), which each harbor a significant ORF. We estimated that both RBDs have been introduced into Old World primate genomes 35 MYA ago, after the separation with New World monkeys. HERV-89 and HERV-41 are included within retroviral elements that comprise potential primer binding sites (PBS) complementary to tRNALeu or tRNAArg, for HERV-89, and tRNAGlu, for HERV-41. The envs of HERV-89 and HERV-41 do not share more than 38% and 69% amino acid identity with the closest known HERVs, respectively, which indicates that they belong to two new Env families. We derived a soluble HERV-89 RBD and monitored its receptor cell and tissue distribution. Using the ligand by flow cytometry, we observed that a HERV-89 receptor is expressed in a large panel of established cell lines and stem cells. Immunohistochemistry on 94 healthy and tumor human tissue samples showed that HERV-89 receptor is largely distributed, with distinct expression patterns in healthy and tumor tissues. In parallel, we derived a 170 gene-containing SLC expression library for high throughput screening of SLC/ligand interactions. Using this partial human SLC library, we identified the long-sought receptor for bovine leukemia virus (BLV). Moreover, transfection of a cDNA library expression into hamster cells, led us to identify CTR1/SLC31A1, the copper and cisplatin transporter, as the receptor for the feline ERV-DC14/FeLV-D.As a ligand for the BLV receptor, BLV-RBD may be used to help controlling BLV transmission and prevent associated pathologies that affect 5% of infected cattle. Also, BLV-RBD and DC14-RBD can now be used as metabolic markers and modulators of their SLC cognate receptors, including copper metabolism, in the case of DC14-RBD
Books on the topic "SLH domain"
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Luckett, Tim, and Katherine L. P. Reid. Speech and language therapy in palliative care. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199656097.003.0410.
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Palliative care is an emerging specialty within the field of speech and language therapy (SLT); the discipline is currently under-represented both in specialist services and the research literature. This belies the fact that many patients in the palliative phase suffer problems with swallowing (dysphagia) and communication, the core domains of SLT practice. This chapter provides an overview of difficulties encountered in these domains by people with life-limiting conditions together with common approaches to assessment and management. Assessment and management should be person-centred, integrated into multidisciplinary care, and seek to maintain function via minimal intervention for maximum gain. More research is needed to inform appropriately integrated, person-centred models of SLT provision that enable difficulties with communication and swallowing to be addressed alongside other symptoms and care needs. It seems likely that difficulties in these domains are currently under-identified and under-treated in many cases.
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Al-Yagon, Michal, and Malka Margalit. Hope and Coping in Individuals with Specific Learning Disorder. Edited by Matthew W. Gallagher and Shane J. Lopez. Oxford University Press, 2017. http://dx.doi.org/10.1093/oxfordhb/9780199399314.013.29.
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This chapter reviews and integrates empirical findings regarding hope as a major personal resource among individuals with specific learning disorder (SLD). First, it describes the Diagnostic and Statistical Manual of Mental Disorders (fifth edition; DSM-5) diagnostic criteria for SLD and briefly illustrates the major difficulties that individuals with SLD may experience in the academic, social, emotional, and behavioral domains. Next, it presents an overview of the empirical literature regarding hope as reported by children and adolescents with SLD in different age groups and its relations with additional personal resources such as the sense of coherence and coping with age-appropriate academic and social challenges. Possible factors that may contribute to the lower resources found in SLD and their implications are explored, as well as future research directions and interventional implications.
Book chapters on the topic "SLH domain"
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Martinez-Quiles, Narcisa. "Cytoskeletal Signaling by Src Homology Domain-Containing Adaptor Proteins." In SH Domains, 187–207. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_9.
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Morel, Bertrand, David Ruzafa, and Francisco Conejero-Lara. "SH3 Domains as Suitable Models to Study Amyloid Aggregation." In SH Domains, 1–15. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_1.
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Mishra, Jayshree, and Narendra Kumar. "Structure and Function of Jak3- SH2 Domain." In SH Domains, 209–27. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_10.
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Kurochkina, Natalya A., and Michael J. Iadarola. "Helical Assemblies and SH Domains." In SH Domains, 229–53. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_11.
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Zhuang, Xiaohong, and Liwen Jiang. "SH Domain Proteins in Plants: Roles in Signaling Transduction and Membrane Trafficking." In SH Domains, 17–33. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_2.
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Azuaga, Ana I., and Salvador Casares Atienza. "Versatility of SH3 Domains in the Cellular Machinery." In SH Domains, 35–69. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_3.
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Kamitori, Shigehiro, and Hiromi Yoshida. "Structure-Function Relationship of Bacterial SH3 Domains." In SH Domains, 71–89. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_4.
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Park, Jeong Won, and Sheue-yann Cheng. "Activation of PI3K by Thyroid Hormone Nuclear Receptors." In SH Domains, 91–110. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_5.
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Ren, Siyuan. "SH Domains’ Interaction with SLiMs: Maximizing Adaptivity of Signaling Networks." In SH Domains, 111–31. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_6.
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Kurochkina, Natalya, Udayan Guha, and Zhong Lu. "SH Domains and Epidermal Growth Factor Receptors." In SH Domains, 133–58. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20098-9_7.
Full textConference papers on the topic "SLH domain"
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Pircher, Michael, Franz Felberer, Matthias Salas, Richard Haindl, Bernhard Baumann, Andreas Wartak, and Christoph K. Hitzenberger. "Phase sensitive adaptive optics assisted SLO/OCT for retinal imaging (Conference Presentation)." In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, edited by Joseph A. Izatt, James G. Fujimoto, and Valery V. Tuchin. SPIE, 2016. http://dx.doi.org/10.1117/12.2210923.
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Li, Qi, Gokhan Tur, Dilek Hakkani-Tur, Xiang Li, Tim Paek, Asela Gunawardana, and Chris Quirk. "Distributed open-domain conversational understanding framework with domain independent extractors." In 2014 IEEE Spoken Language Technology Workshop (SLT). IEEE, 2014. http://dx.doi.org/10.1109/slt.2014.7078636.
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Gloor, Stefan, Jose Ojeda, Jean Dahdah, Nikolay Primerov, Marcus Duelk, and Christian Velez. "Combined-SLED source for UHR-OCT and SLO integrated in 14-pin butterfly module." In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, edited by Joseph A. Izatt and James G. Fujimoto. SPIE, 2020. http://dx.doi.org/10.1117/12.2549082.
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Yuanming, Cao, Wang Wendong, Gong Xiangyang, and Que Xirong. "Initiator-Domain-Based SLA Negotiation for Inter-domain QoS-Service Provisioning." In Fourth International Conference on Networking and Services (icns 2008). IEEE, 2008. http://dx.doi.org/10.1109/icns.2008.43.
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López-Fernández, Jesús J., Esther Guerra, and Juan de Lara. "Example-based validation of domain-specific visual languages." In SLE '15: Software Language Engineering. New York, NY, USA: ACM, 2015. http://dx.doi.org/10.1145/2814251.2814256.
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Latombe, Florent, Xavier Crégut, Benoit Combemale, Julien Deantoni, and Marc Pantel. "Weaving concurrency in executable domain-specific modeling languages." In SLE '15: Software Language Engineering. New York, NY, USA: ACM, 2015. http://dx.doi.org/10.1145/2814251.2814261.
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Chamorovskiy, Alexander, Ekaterina V. Andreeva, Vladimir Shidlovski, Andrey Anikeev, Stepan Il'chenko, and Sergei D. Yakubovich. "Highly efficient superluminescent diodes and SLD-based combined light sources of red spectral range for applications in biomedical imaging." In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII, edited by Joseph A. Izatt, James G. Fujimoto, and Valery V. Tuchin. SPIE, 2018. http://dx.doi.org/10.1117/12.2288246.
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Meyers, Bart, Joachim Denil, István Dávid, and Hans Vangheluwe. "Automated testing support for reactive domain-specific modelling languages." In SLE '16: Software Language Engineering. New York, NY, USA: ACM, 2016. http://dx.doi.org/10.1145/2997364.2997367.
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Li, Weitao, Liping Wang, and Guang Yu. "Time Domain Study on the Construction Mechanism of Milling Stability Lobe Diagrams With Multiple Modes." In ASME 2021 16th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/msec2021-60227.
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Abstract The stability lobe diagram (SLD) is an important expression way of milling stability prediction result. The SLD obtained by only selecting the most flexible mode fails to predict the chatter if the milling process is dominated by multiple modes. To reveal the relationship between the SLD with multiple modes and the SLDs corresponding to each single mode, this paper studies the construction mechanism of the SLD with multiple modes by using the time domain method. First, the milling dynamic model of the tool with multiple modes is established. Then, the numerical method based on the Newton-Cotes rules is used to solve the milling dynamic model with multiple modes whose solution is in the form of the SLD. It shows that the SLD with multiple modes can be approximated by using the lowest envelope of the SLDs corresponding to each single mode. Finally, two study cases are adopted to verify the construction mechanism of the SLD with multiple modes. To verify the correctness of the SLD with multiple modes, a series of milling tests are carried out. The experimental results agree with the simulation results, which means the proposed time domain method can reveal the construction mechanism of the SLD with multiple modes.
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Goncharenko, Boryana, and Vadim Zaytsev. "Language design and implementation for the domain of coding conventions." In SLE '16: Software Language Engineering. New York, NY, USA: ACM, 2016. http://dx.doi.org/10.1145/2997364.2997386.
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