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1

Sanders, Timothy H., Paul D. Blankenship, Richard J. Cole, and Robert A. Hill. "Temperature Relationships of Peanut Leaf Canopy, Stem, and Fruit in Soil of Varying Temperature and Moisture." Peanut Science 12, no. 2 (July 1, 1985): 86–89. http://dx.doi.org/10.3146/pnut.12.2.0010.

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Abstract Physiological processes of plants are affected by temperature and temperature variation of individual plant parts has been demonstrated to affect such physiological interactions as source-sink relationships. Determination of plant part temperatures in relation to the surrounding environment, especially during stress, may provide significant information relative to how plants respond to various stress environments. To determine peanut plant part temperatures in various environments, rainfall control research plots equipped either with heating cables or cooling coils were utilized to grow Florunner peanuts and implement treatments of various soil temperatures under water stress and irrigated conditions. Peanut stem and pod temperatures were monitored automatically at 2-hr intervals with attached and implanted thermocouples. Canopy temperatures, determined by infrared thermometry, were related to water stress but were apparently unrelated to varying soil tempertures. Late-season, afternoon (1:00 p.m.) canopy temperature in the irrigated treatment averaged 28.5 C and mean canopy temperatures in all water stressed treatments were 35±1 C. Late-season plant stem temperature/soil temperature means in irrigated, water stressed-heated soil, water stressed, and water stressed-cooled soil treaments were 21.6 C/21.6 C, 25.2 C/30.2 C, 25.0 C/ 25.C, and 23.3 C/ 20.6 C, respectively. Peanut pod temperatures ranged higher and lower than soil temperature in each plot and maximum pod temperatures often occurred earlier than maximum soil temperature. Concurrent pod, stem, and air maximum and minimum temperatures suggest the strong influence of aerial plant-part temperatures on temperatures of the subterranean fruit. The results of this study show the effect of moisture and temperature stress on peanut plant part temperatures and demonstrate the relationships which result from the unique subterranean fruiting habit.
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2

Vlčková, V., M. Malinová, B. Koubková, J. Száková, V. Zídek, A. Fučíková, and J. Zídková. "Long-term effect of diet amended by risk elements contaminated soils on risk element penetration and physiological parameters of rats." Czech Journal of Animal Science 59, No. 9 (October 1, 2014): 416–27. http://dx.doi.org/10.17221/7653-cjas.

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The long-term accumulation of risk elements (As, Cd, Pb) originated from differently contaminated soils in rat organism was investigated during a model two-generation experiment. The effect of soil contamination level, gender, and length of exposure as well as the interactions between risk elements and selected essential macro- and microelements were studied. Rat diet contained 10% of individual soils (based on dry weight): (i) Fluvisol heavily polluted by As, Cd, Zn, and Pb, (ii) Luvisol contaminated by As, Cd, and Zn, and (iii) uncontaminated Chernozem. Male and female Wistar rats used for the experiment were housed in cages in a room with controlled temperature for 60 days and were fed ad libitum the mentioned diets. Subsequently, the pregnant females were continuously fed the experimental diet until weaning when the young animals were separated to male and female and fed the experimental diet till day 110 of age. The element contents in rat tissues reflected the risk element contents in contaminated soils. The bioaccessibility and bioavailability of the risk elements decreased in the order Cd>As>Pb and was affected by the soil physicochemical parameters. No significant differences were observed between male and female rats as well as between the first and the second generation. However, interactions were reported among the risk elements where the high cadmium content in Fluvisol resulted in increasing arsenic accumulation in the rat liver. Moreover, arsenic–copper interactions were observed where significant increase of the copper level was determined in kidney of the animals fed Luvisol exceeding 50-fold the maximum permissible limits for As content in agricultural soils. Among the hematological and biochemical characteristics of rats, total erythrocyte count (Er), hematocrit (Hct) increased confirming adverse effect of soil-derived risk elements especially in male rats.  
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3

Sherwood, Jill, Diane Debinski, and Matthew Germino. "Testing the Effects of Simulated Climate Change Effects Using Open Sided Warming Chambers." UW National Parks Service Research Station Annual Reports 33 (January 1, 2011): 147–53. http://dx.doi.org/10.13001/uwnpsrc.2011.3811.

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Regional models of global climate change for the northern Rocky Mountains predict warmer temperatures, and some of the main implications of these changes at a local level involve decreased snowpack, earlier snowmelt, and decreased soil moisture during the growing season. In order to mimic the anticipated effects of climate change, and test the responses from a soil microclimate and plant physiology perspective, open-sided warming chambers and snow removal treatments were applied to 2.44 X 2.44 m plots in a sagebrush steppe meadow within Grand Teton National Park, WY. Four treatments included: (1) control, (2) reduced snowpack, (3) increased temperature, and (4) reduced snowpack with increased temperature. Snow was removed using shovels in early May, and chambers were placed at the same time. The chambers were left on the plots through mid-October. Soil moisture and temperature were measured and recorded at 5 cm, and 25 cm depths using dataloggers set up at the time of snow removal and chamber placement. In addition, surface temperature was measured under each plot and within the study area. Plant physiological data on four plant species, including leaf temperature at dawn and mid-afternoon and water potential, were collected for all of the plots in July. Data are being analyzed to determine whether differences existed between the plots for soil moisture, soil and air temperature, and the plant physiological traits measured.
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4

Piotrowski, Krzysztof, and Zdzisława Romanowska-Duda. "Positive impact of bio-stimulators on growth and physiological activity of willow in climate change conditions." International Agrophysics 32, no. 2 (April 1, 2018): 279–86. http://dx.doi.org/10.1515/intag-2017-0006.

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Abstract The aim of this research was to evaluate the physiological activity and growth of willow (Salix viminalis L.) plants cultivated under the conditions of adverse temperature and soil moisture content, and to assess the effect of the foliar application of Biojodis (1.0%) and Asahi SL (0.03%) bio-stimulators, or a mixture of Microcistis aeruginosa MKR 0105 and Anabaena PCC 7120 cyanobacteria under such changing growth conditions. The obtained results showed different reactions to the applied constant or periodically changed temperature and soil moisture content. The plants which grew at periodically changed adverse temperature (from -5 to 40oC) or in scantily (20% m.c.) or excessively (60% m.c.) watered soils, grew slowly, in comparison with those growing at 20oC and in optimally moistened soil (30% m.c.). Foliar application of Biojodis and Asahi SL cyanobacteria increased the growth of willow at optimal and adverse temperature or in scantily and excessively moistened soil. The changes in plant growth were associated with the changes in electrolyte leakage, activity of acid or alkaline phosphatases, RNase, index of chlorophyll content in leaves and gas exchange. The above indicates that the foliar application of the studied cyanobacteria and bio-stimulators partly alleviates the harmful impact of adverse temperature and water stress on growth and physiological activity of willow plants
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5

SHEPPARD, S. C., and G. J. RACZ. "SHOOT AND ROOT RESPONSE OF WHEAT TO BAND AND BROADCAST PHOSPHORUS AT VARYING SOIL TEMPERATURES." Canadian Journal of Soil Science 65, no. 1 (February 1, 1985): 79–88. http://dx.doi.org/10.4141/cjss85-009.

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Response of spring wheat (Triticum aestivum) to varied amounts of broadcast and band-applied phosphorus (P) was examined at soil temperatures of 10, 15, 20 and 25 °C. The research emphasized the response of shoot yields with time and the root proliferation in the band-applied P. A non-destructive technique to measure growth with time was developed. This method allowed interpretation of plant responses even though the temperature treatments markedly changed the rate of physiological development. The relative plant-shoot response to P application did not change with time or developmental stage in this experiment. There was a marked effect of temperature on plant response to band-applied P. Band application was more efficient than broadcast P at 10 °C soil temperature, but less efficient at 25 °C soil temperature. Root proliferation in the fertilizer band was significantly different from the control soil-zone only at 10 °C and was not diminished by concurrent application of broadcast P. The efficiency of band application was confirmed with 32P labelling of the banded P. Banded P accounted for more of the total plant P at lower soil temperatures than at higher soil temperatures, regardless of concurrent application of broadcast P. Temperature had little effect on yield response to broadcast P at the final sampling or throughout the growth period. Tissue P concentrations decreased with increases in temperature and with time. Key words: Temperature, root proliferation, band, broadcast, phosphorus
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6

Pereira, Renata Cristiane, Murilo Fuentes Pelloso, Larissa Vinis Correia, Thaisa Cavalieri Matera, Rayssa Fernanda dos Santos, Alessandro Lucca Braccini, Géssica Gaboardi De Bastiani, Carla Coppo, and Breno Gabriel da Silva. "Physiological quality of soybean seeds treated with imidacloprid before and after storage." Plant, Soil and Environment 66, No. 10 (October 1, 2020): 513–18. http://dx.doi.org/10.17221/364/2020-pse.

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This study aimed to evaluate the effect of different slurry volume of imidacloprid insecticide on the physiological potential during the storage of seeds of three soybean cultivars. A completely randomised experimental design was adopted, in a 6 × 3 × 2 factorial scheme, which treatments were six slurry volumes with imidacloprid insecticide (0, 200, 500, 800, 1 100 and 1 400 mL/100 kg of seeds), 3 soybean cultivars (SYN15630, M5947, and NS5959) and 2 storage periods (0 and 30 days after seed treatment), with four replications. The treated seeds were stored in kraft paper bags with controlled temperature and humidity. The physiological potential of the seeds was evaluated by standard germination test, electrical conductivity, accelerated aging, seedling emergence in the sand seedbed, and the field. The germination and vigor of soybean seeds were reduced during storage, especially with the increase in the volumes of the insecticide solution.
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7

Wang, Jian-Jun, Wei-Hu Lin, Yan-Ting Zhao, Cheng Meng, An-Wei Ma, Long-Hai Xue, Yu Kuang, and Pei Tian. "Physiological and biochemical responses of Festuca sinensis seedlings to temperature and soil moisture stress." Functional Plant Biology 44, no. 10 (2017): 1007. http://dx.doi.org/10.1071/fp16410.

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The interaction effects between temperature and soil moisture on Festuca sinensis Keng ex E.B.Alexeev were analysed to determine how F. sinensis responds to these environmental conditions. A pot experiment was conducted in a greenhouse under simulated growth conditions with four soil moisture contents (80, 65, 50 and 35% relative saturation moisture content) and three temperature conditions (15, 20 and 25°C). Physiological (relative water content and root activity) and biochemical parameters (chlorophyll, peroxidase (POD), malondialdehyde (MDA), soluble protein, soluble sugar and free proline) were evaluated at the seedling stage. Results showed that with a decrease in soil water content, the POD activities, MDA content, soluble protein content, soluble sugar content and free proline content of plants under the 15°C and 20°C treatments initially decreased and then increased, whereas they increased with a decrease of soil water content at 25°C. The relative water contents of plants under the three temperature treatments decreased with a decreasing soil moisture content, but then increased temperature significantly reduced the relative water content of the seedlings under low soil water content. The chlorophyll contents of plants under the 25°C treatment decreased with a decrease of soil moisture content, but those of plants under the 15°C and 20°C treatments initially increased and then decreased. The root activities of plants under the 15°C and 20°C treatments increased with a decreasing soil moisture content; however, those of plants under the 25°C treatment initially increased and then decreased. Thus, results indicated that changes of temperature and soil moisture content had significant and complicated effects on the physiological-biochemical characteristics of F. sinensis; the conditions of 20°C and 65% RSMC had positive effects on F. sinensis seedling growth and the appropriate drought stress could promote the growth of seedling roots under the three different temperature conditions. In conclusion, F. sinensis seedlings could adapt to certain changes in the ecological environment by regulating their physiological and biochemical reactions.
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8

Venturini, Marcela Tonini, Vanderlei da Silva Santos, and Eder Jorge de Oliveira. "Procedures for evaluating the tolerance of cassava genotypes to postharvest physiological deterioration." Pesquisa Agropecuária Brasileira 50, no. 7 (July 2015): 562–70. http://dx.doi.org/10.1590/s0100-204x2015000700006.

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Abstract: The objective of this work was to define procedures to assess the tolerance of cassava genotypes to postharvest physiological deterioration (PPD) and to microbial deterioration (MD). Roots of six cassava genotypes were evaluated in two experiments, during storage under different environmental conditions: high temperature and low soil moisture; or low temperature and high soil moisture. Roots were treated or not with fungicide (carbendazim) before storage. Genotype reactions to MD and PPD were evaluated at 0, 2, 5, 10, 15, 20, and 30 days after harvest (DAH), in the proximal, medial, and distal parts of the roots. A diagrammatic scale was proposed to evaluate nonperipheral symptoms of PPD. Fungicide treatment and root position did not influence PPD expression; however, all factors had significant effect on MD severity. Genotypes differed as to their tolerance to PPD and MD. Both deterioration types were more pronounced during periods of higher humidity and lower temperatures. The fungicide treatment increased root shelf life by reducing MD severity up to 10 DAH. Whole roots showed low MD severity and high PPD expression up to 10 DAH, which enabled the assessment of PPD without significant interference of MD symptoms during this period.
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9

He, Jun Qi, Xue Feng Yuan, and You Ke Wang. "Effect of Soil Moisture Conserving Irrigation under Straw Mulching on Soil and Crop." Applied Mechanics and Materials 71-78 (July 2011): 2315–22. http://dx.doi.org/10.4028/www.scientific.net/amm.71-78.2315.

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Currently, the agricultural water shortage in China has become more and more serious. It is of great importance for scientists and engineers to figure out the ways to utilize the limited water resources efficiently for irrigation. This paper proposes a new irrigation concept which combines advanced soil moisture conserving techniques available in the semi-arid and arid regions of China with the design of water-saving irrigation systems. To show the effectiveness of the new concept, straw mulching used widely in China, a soil moisture conserving technique, was tested in irrigation systems. The experimental results show the way soil moisture, soil microorganism, soil temperature, ecological and physiological indices and maize productivity were affected by straw mulching under irrigation and the improved performance indices with the combination of soil moisture conserving techniques with irrigation systems, which contributed to a higher water use efficiency. It is believed that the combination of soil moisture conserving techniques with irrigation systems will be a new trial for the water-saving irrigation of China, particularly for the semi-arid and arid areas.
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10

Pérez-Hernández, Rosa Guadalupe, Manuel Jesus Cach-Pérez, Rosaura Aparacio-Fabre, Hans Van der Wal, and Ulises Rodríguez-Robles. "Physiological and microclimatic consequences of variation in agricultural management of maize." Botanical Sciences 99, no. 1 (October 27, 2020): 132–48. http://dx.doi.org/10.17129/botsci.2640.

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Background: Maize is cultivated under different agricultural management systems, which influence the ecological dynamics of the crop, and therefore the physiology of the plant. Questions: What is the effect of different agricultural management on the microclimate and the physiology of maize plants? Studied species: Zea mays L. Study site and dates: Nacajuca, Tabasco, Mexico; January to April 2017. Methods: Physiological performance of maize plants and microclimatic variation in the crop area was characterized under three management systems: maize monoculture, maize-bean, and maize-bean-squash intercropping. Each treatment was established in three 100 m2 plots (300 m2 per treatment). Four measurements were taken between days 33 and 99 after maize sowing, to characterize five microclimatic parameters (relative air humidity, air and soil temperature, vapor-pressure deficit and soil volumetric water content) and nine physiological parameters (photosynthesis, transpiration, water use efficiency, stomatal conductance, electron transport rate, quantum efficiency of photosystem II, non-photochemical quenching, foliar water potential and chlorophyll content). Results: Maximum soil temperature was up to 4.4 ºC less in the maize-bean system than in the monoculture at 15:00 h; soil in the maize-bean-squash intercropping retained up to 45 % more water than the monoculture throughout the day. Photosynthesis and electron transport rate in the maize-bean intercropping was up to 32 % higher than in the monoculture. The highest non-photochemical quenching and transpiration rate were observed in the maize-bean-squash system. Conclusions: The maize-bean and maize-bean-squash combination provides maize plants with lower soil temperature and higher water availability, allowing them better physiological performance compared to monoculture.
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11

Chillet, M., O. Hubert, M. J. Rives, and L. de Lapeyre de Bellaire. "Effects of the Physiological Age of Bananas on Their Susceptibility to Wound Anthracnose Due to Colletotrichum musae." Plant Disease 90, no. 9 (September 2006): 1181–85. http://dx.doi.org/10.1094/pd-90-1181.

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Wound anthracnose, caused by Colletotrichum musae, and early ripening are the main problems affecting the quality of export bananas (Musa AAA Cavendish) from the Caribbean. These problems generally concern bananas grown in lowland plantations during the rainy season. Three experiments were carried out to study the influence of the physiological age of bananas, calculated on the basis of mean daily temperature sums, on their susceptibility to anthracnose. Stressful growing conditions, especially soil flooding, slowed fruit growth but had no direct effect on fruit susceptibility to C. musae or on the green life. However, fruit that had accumulated lower temperature sums were less susceptible to wound anthracnose. By varying the source-sink ratio, we show that bananas of the same grade but different physiological ages had markedly different susceptibility to C. musae. Bananas with the same temperature sum accumulation but grown in different soil-climate conditions had different levels of susceptibility. Fruit grown in cooler, highland areas were less susceptible to C. musae than fruit of the same physiological age from lowland plantations. Our results suggest that temperature sum accumulation rate is a critical factor affecting the susceptibility of bananas to the pathogen.
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12

Backman, Agneta, Ninwe Maraha, and Janet K. Jansson. "Impact of Temperature on the Physiological Status of a Potential Bioremediation Inoculant, Arthrobacter chlorophenolicus A6." Applied and Environmental Microbiology 70, no. 5 (May 2004): 2952–58. http://dx.doi.org/10.1128/aem.70.5.2952-2958.2004.

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ABSTRACT Arthrobacter chlorophenolicus A6 (A6) can degrade large amounts of 4-chlorophenol in soil at 5 and 28°C. In this study, we investigated the effects of temperature on the physiological status of this bacterium in pure culture and in soil. A derivative of A6 tagged with the gfp gene (encoding green fluorescent protein [GFP]) was used to specifically quantify A6 cells in soil. In addition, cyano-ditolyl-tetrazoliumchloride was used to stain GFP-fluorescent cells with an active electron transfer system (“viable cells”) whereas propidium iodide (PI) was used to stain cells with damaged membranes (“dead cells”). Another derivative of the strain (tagged with the firefly luciferase gene [luc]) was used to monitor the metabolic activity of the cell population, since the bioluminescence phenotype is dependent on cellular energy reserves. When the cells were incubated in soil at 28°C, the majority were stained with PI, indicating that they had lost their cell integrity. In addition, there was a corresponding decline in metabolic activity and in the ability to be grown in cultures on agar plates after incubation in soil at 28°C, indicating that the cells were dying under those conditions. When the cells were incubated in soil at 5°C, by contrast, the majority of the cells remained intact and a large fraction of the population remained metabolically active. A similar trend towards better cell survival at lower temperatures was found in pure-culture experiments. These results make A. chlorophenolicus A6 a good candidate for the treatment of chlorophenol-contaminated soil in cold climates.
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13

Sarath, Karina Laís Leite, André Luís Duarte Goneli, Cesar Pedro Hartmann Filho, Tathiana Elisa Masetto, and Guilherme Cardoso Oba. "Physiological potential of peanut seeds submitted to drying and storage." Journal of Seed Science 38, no. 3 (September 15, 2016): 233–40. http://dx.doi.org/10.1590/2317-1545v38n3165008.

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Abstract: This study was performed to evaluate the immediate and latent effects of drying air temperature on the physiological potential of peanuts (Arachis hypogaea L.). The fruits were harvested at the physiological maturity stage, with 36 ± 2% water content (w.b.), and dried at temperatures of 40, 50, 60 and 70 ºC, until 7 ± 1% (w.b.). Thereafter, the fruits were stored in an environment with non-controlled temperature and relative humidity for 150 days, and the water content, germination and vigor of seeds were immediately determined, as well as at every 30 days thereafter, through the first count, and the tetrazolium test, accelerated aging test, cold test with soil and electrical conductivity. According to the results, it was found that the physiological potential of peanut seeds is inversely related to the drying temperature and the storage time, due to the fact that the germination and vigor are reduced as both factors increase. The air temperature of 40 °C may be recommended for the drying of peanut seeds, and even associated with the storage time of 150 days under uncontrolled conditions.
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14

Cardoso, Antonio Ismael Inácio, Laura Z. Piacenti, Priscilla R. Lino, Iasmim M. Padovan, and Adriana Z. Kronka. "Control of Alternaria brassicicola with thermotherapy and propolis and effect on the physiological quality of kale seeds." Horticultura Brasileira 38, no. 4 (December 2020): 363–69. http://dx.doi.org/10.1590/s0102-053620200404.

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ABSTRACT In the organic production system, the use of agrochemicals for seed treatment is prohibited. This study was carried out to evaluate the effect of heat treatment and propolis on the germination and vigor of kale seeds and the effect on the control of Alternaria brassicicola. In the treatment with wet thermotherapy, three temperatures (50, 55 and 60°C) were evaluated in separated experiments. For each temperature, five immersion times [0 (control), 15, 30, 45 and 60 minutes] were evaluated. In the dry thermotherapy treatment, seeds were submitted to constant 70ºC temperature and five times [0 (control), 24, 48, 72 and 96 hours] were evaluated. In the treatment with propolis, six concentrations (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0%) were studied, with subsequent washing or not washing of the seeds. Then, seeds were inoculated with A. brassicicola and submitted to the following treatments: wet thermotherapy at 50°C for 60 minutes and at 55°C for 15 minutes and propolis at 1.0% with and without further washing. Two control treatments were included in the experiment (inoculated untreated seeds; and not inoculated and untreated seeds), totalizing six treatments. After inoculation and treatments, the incidence of the fungus on the seeds was evaluated and the effect on the physiological quality of the seeds. Both thermotherapy treatments (50°C for 60 minutes and 55°C for 15 minutes) reduced the pathogen incidence significantly. Both propolis treatments at 1% (with and without subsequent washing) did not provide a satisfactory reduction on A. brassicicola incidence in kale seeds. Thermotherapy at 55°C for 15 minutes is the best option for A. brassicicola control in kale seeds, because it does not affect seed physiological quality.
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15

Boczulak, S. A., B. J. Hawkins, D. G. Maynard, and R. Roy. "Long- and short-term temperature differences affect organic and inorganic nitrogen availability in forest soils." Canadian Journal of Soil Science 95, no. 2 (May 2015): 77–86. http://dx.doi.org/10.4141/cjss-2014-085.

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Boczulak, S. A., Hawkins, B. J., Maynard, D. G. and Roy, R. 2015. Long- and short-term temperature differences affect organic and inorganic nitrogen availability in forest soils. Can. J. Soil Sci. 95: 77–86. Soil microbial activity determines rates of decomposition and is strongly influenced by temperature. Soil microbial communities may be adapted to site characteristics, including temperature, through physiological modification of microbial populations or changes in species composition; however, response to short-term changes in temperature may also occur. We searched for evidence of short- and long-term temperature response of microbial communities involved in soil nitrogen (N) cycling by measuring the relative availability of organic and inorganic N forms in forest soils from a high and a low elevation site, incubated at 10, 16 and 20°C for 16 wk. By week 16, ammonium concentrations were greater in soils incubated at 16 and 20°C than at 10°C, and in soil from the low elevation site, compared with high elevation. Nitrate concentrations increased in soil from the low elevation site incubated at 16 and 20°C, but changed little in other treatments. Assessment of autotrophic nitrification potential showed gross nitrification in soil from the low elevation site was likely from classical chemolithotrophic nitrifiers. Organic N concentration increased over time in the 16 and 20°C incubations of soil from the low elevation site, but only increased in the 20°C treatment for soil from the high elevation site. Long-lasting site effects were indicated by the more active microbial community in soil from low elevation, which could be related to site temperature. Evidence of short-term temperature response of N cycling processes was observed in soils from both elevations.
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Rostamian, Mohammad, Mohammad Reza Kavosi, Edi Bazgir, and Manoochehr Babanezhad. "Investigation of physiological changes in the affected Quercus brantii stand by oak charcoal disease." Journal of Forest Science 65, No. 3 (March 30, 2019): 106–12. http://dx.doi.org/10.17221/107/2018-jfs.

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The purpose of this work was to monitor the effects of the environmental factors include temperature, precipitation and sun radiation on some physiological aspects of Q. brantii trees in the forest stand involved with the oak charcoal disease during a growing season. We designed a systematic random sampling and all trees were examined for disease status. Our result showed that 70.96% of the trees with different intensities are affected by the charcoal disease. Accordingly, values of predawn leaf water potential (PWP), midday leaf water potential (MWP) and chlorophyll variables showed significant difference in classes of oak charcoal disease. Linear regression analysis showed that the values of PWP, MWP and chlorophyll are changing at the high temperature. Mean of canker length are 20.5 cm and 51.7 cm in class 2 and 3 respectively and there is a great relation between PWP value and canker length (r<sup>2</sup> = 0.914). Canker length also has a linear relation with MWP values (r<sup>2</sup> = 0.627). Drought stress affected the physiological functions of oak trees and considerably reduced their defense potential against pathogen agents.
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17

Kabir, Mohammed Humayun, Qing Liu, Qiong Peng, Zhigang Huang, Bin Hong, Hesong LI, and Langtao Xiao. "Effect of temperature on chalkiness and related physiological and biochemical characteristics of earlyindicarice during grain filling." Research on Crops 15, no. 2 (2014): 313. http://dx.doi.org/10.5958/2348-7542.2014.00116.8.

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18

Chinsamy, Mayashree, Manoj G. Kulkarni, and Johannes Van Staden. "Vermicompost Leachate Reduces Temperature and Water Stress Effects in Tomato Seedlings." HortScience 49, no. 9 (September 2014): 1183–87. http://dx.doi.org/10.21273/hortsci.49.9.1183.

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Abiotic stresses negatively affect the rate of nutrient mobilization in soils resulting in poor crop performance. Vermicompost-leachate (VCL) is an organic liquid produced from earthworm-digested material. It improves soil fertility as a result of the presence of proteins, vitamins, and micro- and macroelements. However, the effects of VCL on plant growth under temperature and water stress are not yet fully studied. To assess the growth performance under low, optimum, and high temperatures, 1-month-old well established tomato seedlings were treated with and without VCL (1:10 v/v) under various temperature regimes (10, 15, 20, 25, and 30 °C). In the second trial, tomato seedlings were tested in a greenhouse with and without VCL (1:10 v/v) at different watering regimes (15, 30, and 45 mL of Hoagland’s nutrient solution) to evaluate the effect of water stress. In comparison with the control seedlings, VCL treatment significantly improved stem thickness, leaf area, and shoot/root both fresh and dry weight of seedlings at 30 °C. At this temperature, VCL-treated seedlings showed a significant increase for all examined physiological parameters (total chlorophyll, total sugars, and proline). Number of leaves, stem thickness, and shoot/root length and fresh weight of VCL-treated tomato seedlings irrigated under a low watering regime were significantly greater than the control. Total chlorophyll, total sugars, and proline content were significantly elevated at the high watering regime but declined in the low watering regime with VCL treatment. Both increasing and decreasing trends of compatible solutes and photosynthetic pigments indicated osmotic adjustment to stress conditions. VCL can be a suitable soil amendment product to improve overall soil fertility and, more importantly, growth of tomato plants even under temperature and water stress conditions.
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19

Notaro, Michael, Steve Vavrus, and Zhengyu Liu. "Global Vegetation and Climate Change due to Future Increases in CO2 as Projected by a Fully Coupled Model with Dynamic Vegetation*." Journal of Climate 20, no. 1 (January 1, 2007): 70–90. http://dx.doi.org/10.1175/jcli3989.1.

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Abstract Transient simulations are presented of future climate and vegetation associated with continued rising levels of CO2. The model is a fully coupled atmosphere–ocean–land–ice model with dynamic vegetation. The impacts of the radiative and physiological forcing of CO2 are diagnosed, along with the role of vegetation feedbacks. While the radiative effect of rising CO2 produces most of the warming, the physiological effect contributes additional warming by weakening the hydrologic cycle through reduced evapotranspiration. Both effects cause drying over tropical rain forests, while the radiative effect enhances Arctic and Indonesian precipitation. A global greening trend is simulated primarily due to the physiological effect, with an increase in photosynthesis and total tree cover associated with enhanced water-use efficiency. In particular, tree cover is enhanced by the physiological effect over moisture-limited regions. Over Amazonia, South Africa, and Australia, the radiative forcing produces soil drying and reduced forest cover. A poleward shift of the boreal forest is simulated as both the radiative and physiological effects enhance vegetation growth in the northern tundra and the radiative effect induces drying and summertime heat stress on the central and southern boreal forest. Vegetation feedbacks substantially impact local temperature trends through changes in albedo and evapotranspiration. The physiological effect increases net biomass across most land areas, while the radiative effect results in an increase over the tundra and decrease over tropical forests and portions of the boreal forest.
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Duan, Honglang, Josephine Ontedhu, Paul Milham, James D. Lewis, and David T. Tissue. "Effects of elevated carbon dioxide and elevated temperature on morphological, physiological and anatomical responses of Eucalyptus tereticornis along a soil phosphorus gradient." Tree Physiology 39, no. 11 (November 1, 2019): 1821–37. http://dx.doi.org/10.1093/treephys/tpz094.

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Abstract Eucalypts are likely to play a critical role in the response of Australian forests to rising atmospheric CO2 concentration ([CO2]) and temperature. Although eucalypts are frequently phosphorus (P) limited in native soils, few studies have examined the main and interactive effects of P availability, [CO2] and temperature on eucalypt morphology, physiology and anatomy. To address this issue, we grew seedlings of Eucalyptus tereticornis Smith across its P-responsive range (6–500 mg kg−1) for 120 days under two [CO2] (ambient: 400 μmol mol−1 (Ca) and elevated: 640 μmol mol−1 (Ce)) and two temperature (ambient: 24/16 °C (Ta) and elevated: 28/20 °C (Te) day/night) treatments in a sunlit glasshouse. Seedlings were well-watered and supplied with otherwise non-limiting macro- and micro-nutrients. Increasing soil P supply increased growth responses to Ce and Te. At the highest P supplies, Ce increased total dry mass, leaf number and total leaf area by ~50%, and Te increased leaf number by ~40%. By contrast, Ce and Te had limited effects on seedling growth at the lowest P supply. Soil P supply did not consistently modify photosynthetic responses to Ce or Te. Overall, effects of Ce and Te on growth, physiological and anatomical responses of E. tereticornis seedlings were generally neutral or negative at low soil P supply, suggesting that native tree responses to future climates may be relatively small in native low-P soils in Australian forests.
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Wibowo, Febri Arif Cahyo, Rosa Septiana Mieske Putri, Amir Syarifuddin, and Tatag Muttaqin. "SISTEM PERBAIKAN EKOFISIOLOGI TANAMAN SENGON (Paraserianthes falcataria (L) Nielsen ) DI KECAMATAN WAGIR, 161 MALANG." Jurnal Hutan Tropis 8, no. 2 (July 5, 2020): 161. http://dx.doi.org/10.20527/jht.v8i2.9063.

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Sengon plant (Paraserianthes falcataria (L) Nielsen) in Indonesia is one of the types of plants developed in the development of Community Forestry especially in Wagir District, Malang Regency. This study was conducted to analyze the effect of environmental conditions, especially temperature and humidity on stomata density and chlorophyll levels in the plant Sengon (Paraserianthes falcataria (L) Nielsen) and analyze the soil nutrient content under the Sengon stand (Paraserianthes falcataria (L) Nielsen) on plant physiology in Sukodadi Village, Wagir District, Malang Regency. The techniques used are field surveys, leaf sampling to measure physiological properties (stomata density and chlorophyll content), and soil sampling to measure soil chemical properties (macro nutrient content), as well as direct observations in the field to measure environmental factors (temperature and humidity). Analysis of the data used is multiple linear regression in SPSS to determine whether there is influence of temperature and humidity on the physiology of stomata and chlorophyll as well as descriptive analysis on soil factors. Research result is that temperature and humadity have an effect on chlorophyll. This support to possibility of temperature and humadity system in the region so that agreed crops will affect the concentration of chlorine.
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Schmidt, C. S., F. Agostini, C. Leifert, K. Killham, and C. E. Mullins. "Influence of Soil Temperature and Matric Potential on Sugar Beet Seedling Colonization and Suppression of Pythium Damping-Off by the Antagonistic Bacteria Pseudomonas fluorescens and Bacillus subtilis." Phytopathology® 94, no. 4 (April 2004): 351–63. http://dx.doi.org/10.1094/phyto.2004.94.4.351.

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Pseudomonas fluorescens B5 and Bacillus subtilis MBI 600 colonized sugar beet seedlings at matric potentials of -7 × 103, -140 × 103, and -330 × 103 Pa and under five temperature regimes ranging from 7 to 35°C, with diurnal fluctuations of 5 to 22°C. No interaction between matric potential and temperature was observed. In situ bioluminescence indicated physiological activity of Pseudomonas fluorescens B5. Colonization of the root at ≥4 cm below the seed decreased at very low matric potential (-330 × 103 Pa). Total population size of Pseudomonas fluorescens B5 per seedling was significantly increased at -140 × 103 Pa. However, matric potential had no significant effect on the population density of Pseudomonas fluorescens per gram of root fresh weight and did not affect the distribution of the population down the root. Total population size per seedling and downward colonization by Pseudomonas fluorescens B5 were significantly reduced at high temperatures (25 to 35°C). Maximum colonization down the root occurred at intermediate temperature (15°C) at both matric potentials (-7 × 103 and -140 × 103 Pa). Addition of B. subtilis MBI 600 to the seed had no effect on rhizosphere populations of Pseudomonas fluorescens B5. Populations of B. subtilis MBI 600, which consisted largely of spores, were slightly reduced at lower matric potentials and were not affected by temperature. Survival and dry weight of plants in soils infested with Pythium spp. decreased with increasing soil temperature and matric potential, indicating an increase in disease pressure. However, there was no significant interaction between the two factors. At -330 × 103 Pa, soil dryness but not Pythium infection was the limiting factor for plant emergence. At temperatures of 7 to 25°C and matric potentials of -7 × 103 to 120 × 103 Pa, treatment with Pseudomonas fluorescens B5 increased plant survival and dry weight. At 7°C and -120 × 103 Pa, there was almost complete emergence of seeds treated with Pseudomonas fluorescens B5. Antagonistic activity of Pseudomonas fluorescens B5 decreased with increasing soil temperature and decreasing matric potential. At 25 to 35°C and -7 × 103 Pa, no effect was observed. In regimes with different day and night temperatures, the maximum (day) temperature was decisive for disease development and antagonistic activity. B. subtilis MBI 600 displayed no significant antagonistic effect against Pythium ultimum and did not influence the performance of Pseudomonas fluorescens B5 in combined inocula.
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Arief, Omnia M., Jiayin Pang, Kamal H. Shaltout, and Hans Lambers. "Performance of two Lupinus albus L. cultivars in response to three soil pH levels." Experimental Agriculture 56, no. 3 (November 14, 2019): 321–30. http://dx.doi.org/10.1017/s0014479719000383.

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AbstractSoil alkalinity imposes important limitations to lupin productivity; however, little attention has been paid to investigate the effects of soil alkalinity on plant growth and development. Many lupins are sensitive to alkaline soils, but Lupinus albus material from Egypt was found to have tolerance to limed soils. The aim of this study was to compare the growth response of two cultivars of L.albus L. – an Egyptian cultivar, P27734, and an Australian cultivar, Kiev Mutant, to different soil pH levels and to understand the physiological mechanisms underlying agronomic alkalinity tolerance of P27734. Plants were grown under three pH levels (5.1, 6.7, and 7.8) in a temperature-controlled glasshouse. For both cultivars, the greatest dry mass production and carboxylate exudation from roots were observed at alkaline pH. The better performance of the Egyptian cultivar at high pH was entirely accounted for by its greater seed weight. From a physiological perspective, the Australian cultivar was as alkaline-tolerant as the Egyptian cultivar. These findings highlight the agronomic importance of seed weight for sowing, and both cultivars can be used in alkaline soils.
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Puig-Sirera, Àngela, Daniele Antichi, Dylan Warren Raffa, and Giovanni Rallo. "Application of Remote Sensing Techniques to Discriminate the Effect of Different Soil Management Treatments over Rainfed Vineyards in Chianti Terroir." Remote Sensing 13, no. 4 (February 16, 2021): 716. http://dx.doi.org/10.3390/rs13040716.

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The work aimed to discriminate among different soil management treatments in terms of beneficial effects by high-resolution thermal and spectral vegetation imagery using an unmanned aerial vehicle and open-source GIS software. Five soil management treatments were applied in two organic vineyards (cv. Sangiovese) from Chianti Classico terroir (Tuscany, Italy) during two experimental years. The treatments tested consisted of conventional tillage, spontaneous vegetation, pigeon bean (Vicia faba var. minor Beck) incorporated in spring, mixture of barley (Hordeum vulgare L.) and clover (Trifolium squarrosum L.) incorporated or left as dead mulch in late spring. The images acquired remotely were analyzed through map-algebra and map-statistics in QGIS and correlated with field ecophysiological measurements. The surface temperature, crop water stress index (CWSI) and normalized difference vegetation index (NDVI) of each vine row under treatments were compared based on frequency distribution functions and statistics descriptors of position. The spectral vegetation and thermal-based indices were significantly correlated with the respective leaf area index (R2 = 0.89) and stem water potential measurements (R2 = 0.59), and thus are an expression of the crop vigor and water status. The gravel and active limestone soil components determined the spatial variability of vine biophysical (e.g., canopy vigor) and physiological characteristics (e.g., vine chlorophyll content) in both farms. The vine canopy surface temperature, and CWSI were lower on the spontaneous and pigeon bean treatments in both farms, thus evidencing less physiological stress on the vine rows derived from the cover crop residual effect. In conclusion, the proposed methodology showed the capacity to discriminate across soil management practices and map the spatial variability within vineyards. The methodology could serve as a simple and non-invasive tool for precision soil management in rainfed vineyards to guide producers on using the most efficient and profitable practice.
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25

Tužinský, L. "Soil moisture in mountain spruce stand." Journal of Forest Science 48, No. 1 (May 17, 2019): 27–37. http://dx.doi.org/10.17221/11854-jfs.

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Mountain forests are among the main components of natural environment in Slovakia. They grow mainly in areas with cold climate, on poor soils with unfavorable reaction, often very acidic (pH in H<sub>2</sub>O &lt; 4.5) and with nutrient deficit. Immissions and acid rain attack forests to a great extent. Global climate changes also represent a new threat. Extremes in air temperatures, excessive amounts of precipitation or on the other hand the lack of water from precipitation, torrential rains or long-lasting drought periods are recorded as a result of a higher amount of heat energy accumulation from the greenhouse effect. Spruce forests are most endangered. Spruce with its root system concentrated in the upper soil layers, where also the highest amount of toxic elements accumulates, suffers more and more from dry and warm periods and it begins to wither due to drought. The occurrence of hydropedological cycles with a low or insufficient supply of available water in the soil is most frequent during summer (July, August). If the soil water potential values approach the value of the wilting point, an expressive decrease in transpiration is observed during the day, whereas its daily course is also suppressed. Gradual soil drying up from the upper layers towards the deepest ones of the physiological profile of soil represents a change in soil moisture stratification, especially after moistening the upper layers of soil with water from atmospheric precipitation. The deeper soil layers need not be re-saturated in such a case. Under drought the whole physiological profile of soil dries up in a relatively short time. Trees are exposed to a strong physiological stress in such conditions and after longlasting drought periods they can get into the state of total exhaustion.
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26

Choudhary, V. K., M. C. Bhambri, N. Pandey, and H. G. Sharma. "Effect of drip irrigation and mulches on physiological parameters, soil temperature, picking patterns and yield in capsicum (Capsicum annuumL.)." Archives of Agronomy and Soil Science 58, no. 3 (March 2012): 277–92. http://dx.doi.org/10.1080/03650340.2010.517197.

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27

Mahzer, Masooma, Mawra Gohar, and Sumaira Mazher. "Plasmid Profiling and Effect of Different Physiological Parameters on the Chromium Reduction Potential of Microbes." JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS 1, no. 2 (August 28, 2020): 19–31. http://dx.doi.org/10.52700/jmmg.v1i2.14.

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Chromium is toxic for both human and aquatic life. It is recommended to eradicate from wastewaters or to alter its oxidation state to less toxic level The purpose of current research was to isolate heavy metal (Cr) resistant bacteria from different industrial effluents (soil and waste water), to determine their potential for chromium reduction (CRP) at different parameters (time period, pH, temperature and concentrations of chromium) and to determine the plasmid profiles of Cr (VI) resistant bacterial isolates. The growth of chromium resistant bacteria was determined by checking the influence of pH, concentration of chromium, time period and temperature on isolates using UV spectrophotometer, while chromium reduction potential was also investigated using Deleo and Ehrlich method. Plasmid profiling was performed and analyzed using agarose gel electrophoresis (0.8%) to determine the number, size and relationship of plasmid with heavy metal resistance. Results showed that the identified bacterial isolates (S. aureus and S. epidermidis) were resistant to heavy metal (Cr) confirmed by resistance profiling. The maximum growth of bacterial isolate was recorded after 24-hour incubation period (1.154), at pH 8 (1.512), temperature 37ºC (1.615) and 500 µg/mL chromium concentration (1.978), while suitable conditions observed for chromium reduction potential was 24-hour incubation period (57%), pH 7 (62.6%), temperature 30ºC (60%), and 500 µg/mL concentration of chromium (60%). The plasmid profiles revealed that plasmid were randomly distributed among the bacterial isolates with average plasmid number (2.9) ranging from 0-5 and molecular size (100-12000bps). Overall, no defined relationship was observed among resistance pattern and plasmid mediated profiles.
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28

Oryokot, Joseph O. E., Stephen D. Murphy, and Clarence J. Swanton. "Effect of tillage and corn on pigweed (Amaranthusspp.) seedling emergence and density." Weed Science 45, no. 1 (February 1997): 120–26. http://dx.doi.org/10.1017/s0043174500092560.

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We studied the effect of no-till, chisel, and moldboard plow and the presence or absence of corn on soil temperature, moisture and, subsequently, the emergence phenology and density of pigweed seedlings at 2 sites from 1993 to 1995 inclusively. Tillage significantly affected the phenology of pigweed seedling emergence only during a June drought at one site in 1994. Soil temperature and moisture, measured at 2.5-cm depths, also were unaffected by tillage. Weed phenology is usually earlier in no-till because more seeds are located closer to the surface (< 5 cm deep) in no-till, thereby reducing the delay in penetrating through the soil, and because soil temperatures and moisture are nearer the germination and emergence optima. However, pigweed seedlings are already physiologically restricted to germination depths of less than 2.5 cm regardless of tillage; therefore, this prior constraint eliminated any potential differences in emergence phenologies caused by tillage. The presence or absence of corn also did not affect soil temperatures, soil moisture, or pigweed seedling emergence phenologies. Pigweed seedling density was significantly higher in no-till; this may have been caused by increased numbers of seeds near the soil surface in no-till. The presence or absence of corn did not affect pigweed seedling density; the lack of a significant effect probably reflects high variances in density. Although necessary for most weed species, tillage may be a less important factor to consider in predicting pigweed population dynamics and subsequent management recommendations.
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29

Firman, D. M., P. J. O'Brien, and E. J. Allen. "Predicting the emergence of potato sprouts." Journal of Agricultural Science 118, no. 1 (February 1992): 55–61. http://dx.doi.org/10.1017/s0021859600068003.

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SUMMARYSprout emergence in the potato variety Estima was investigated in controlled environment and field experiments. The effects of temperature, physiological age, planting date and soil moisture were examined in controlled environments and the results were compared with the emergence of physiologically young and old seed in field experiments with different soil types, planting dates and planting depths.Elongation of sprouts could be described by a lag period of slow growth followed by a period of rapid linear growth. The lag period was shorter with increase in temperature up to 20 °C and sprout length at planting up to c. 10 mm. The lag period was slightly reduced by warming seed at 10 °C before planting, compared with planting seed directly from cold storage. The rate of linear growth increased with temperature up to 20 °C but was similar for young and old seed at different planting dates. The rate of growth was lower in dry soil than in soil near to field capacity, particularly at high temperatures.The observed date of emergence in the field was usually later than predicted, particularly with shallow planting, but prediction was improved by accounting for soil moisture. The use of this model should give more accurate estimation of emergence for farm operations and crop growth models than existing equations.
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Chang, Yuru, Lorenzo Rossi, Lincoln Zotarelli, Bin Gao, and Ali Sarkhosh. "Greenhouse Evaluation of Pinewood Biochar Effects on Nutrient Status and Physiological Performance in Muscadine Grape (Vitis rotundifolia L.)." HortScience 56, no. 2 (February 2021): 277–85. http://dx.doi.org/10.21273/hortsci15428-20.

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Muscadine grape is a perennial crop that is highly responsive to local environmental factors and viticulture practices. Biochar is a promising soil amendment used to improve soil water and nutrient retention and promote plant growth. The present study aimed to assess the effects of different pinewood biochar rates on nutrient status and vegetative parameters of muscadine grape cv. Alachua grown on a nutrient-poor sandy soil, Ultisols (97.2% sand, 2.4% silt, and 0.4% clay), and mixed with five different rates (0%, 5%, 10%, 15%, and 20%) of biochar based on weight. Variations in soil moisture, temperature, and leaf greenness value [soil plant analysis development (SPAD) reading], net photosynthesis rate, and plant root and shoot dry weights were measured. In addition, the nutrient status of the soil, plant root, and shoot were determined. The results indicated that the higher rate of biochar could significantly (P < 0.05) improve soil moisture. Biochar can also decrease soil temperature, although there were no significant differences among treatments. Regarding the nutrient status, the biochar amendment increased the nutrient content of phosphorus (P), potassium (K), magnesium (Mg), and calcium (Ca), as well as the soil organic matter content and cation exchange capacity. Higher nutrient contents in soil lead to increased P and Mg in both aboveground and belowground muscadine plant tissues and decreased nitrogen (N), iron (Fe), and copper (Cu) in the root part. There were no significant differences observed in SPAD values, net photosynthesis, or dry weights of the root and shoot. This study demonstrates that the addition of biochar may enhance the soil water and nutrient status as well as improve plant P and Mg uptake; however, it showed no significant differences in the physiological performance of muscadine grape plants.
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31

Izquierdo, N. G., L. A. N. Aguirrezábal, E. Martínez-Force, R. Garcés, V. Paccapelo, F. Andrade, R. Reid, and A. Zambelli. "Effect of growth temperature on the high stearic and high stearic-high oleic sunflower traits." Crop and Pasture Science 64, no. 1 (2013): 18. http://dx.doi.org/10.1071/cp12437.

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We investigated variability in the response of oil fatty acid composition to temperature among high stearic and high stearic-high oleic sunflower (Helianthus annuus L.) genotypes. Two experiments were conducted with high stearic (including the CAS-3 mutation) and high stearic-high oleic inbred lines (including both the CAS-3 and the high oleic Soldatov mutations). Plants were cultivated in pots with soil, irrigated, and fertilised. Plants were exposed to different day/night temperatures during grain filling: 16/16°C, 26/16°C, 26/26°C, and 32/26°C. Oil fatty acid composition was determined by gas–liquid chromatography in seeds harvested after physiological maturity. Higher temperature during grain filling increased palmitic and oleic acid percentages and reduced stearic and linoleic acid percentages, suggesting some modifications on enzymatic activities. When the high oleic mutation was included, the variation in stearic and oleic acid percentages in response to temperature was reduced but not the variation in palmitic acid concentration. Variations in fatty acid composition in high stearic genotypes were mainly associated with night temperature as reported previously for traditional and high oleic hybrids. Knowing the effect of temperature on oil fatty acid composition in traditional and mutated genotypes is useful for selecting the environment in which to produce grains with the desired oil quality.
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Vital, Roberto Gomes, Caroline Müller, Fábia Barbosa da Silva, Priscila Ferreira Batista, Andrew Merchant, David Fuentes, Arthur Almeida Rodrigues, and Alan Carlos Costa. "Nitric Oxide Increases the Physiological and Biochemical Stability of Soybean Plants under High Temperature." Agronomy 9, no. 8 (July 26, 2019): 412. http://dx.doi.org/10.3390/agronomy9080412.

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Thermal stress reduces plant growth and development, resulting in considerable economic losses in crops such as soybeans. Nitric oxide (NO) in plants is associated with tolerance to various abiotic stresses. Nevertheless, there are few studies of the range of observed effects of NO in modulating physiological and metabolic functions in soybean plants under high temperature. In the present study, we investigated the effects of sodium nitroprusside (SNP, NO donor), on anatomical, physiological, biochemical, and metabolic processes of soybean plants exposed to high temperature. Soybean plants were grown in soil: sand (2:1) substrate in acclimatized growth chambers. At developmental V3 stage, plants were exposed to two temperatures (25 °C and 40 °C) and SNP (0 and 100 μM), in a randomized block experimental design, with five replicates. After six days, we quantified NO concentration, leaf anatomy, gas exchange, chlorophyll a fluorescence, photosynthetic pigments, lipid peroxidation, antioxidant enzyme activity, and metabolite profiles. Higher NO concentration in soybean plants exposed to high temperature and SNP showed increased effective quantum yields of photosystem II (PSII) and photochemical dissipation, thereby maintaining the photosynthetic rate. Under high temperature, NO also promoted greater activity of ascorbate peroxidase and peroxidase activity, avoiding lipid peroxidation of cell membranes, in addition to regulating amino acid and organic compound levels. These results suggest that NO prevented damage caused by high temperature in soybean plants, illustrating the potential to mitigate thermal stress in cultivated plants.
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Guo, Yuewei, and Yunge Zhao. "Effects of storage temperature on the physiological characteristics and vegetative propagation of desiccation-tolerant mosses." Biogeosciences 15, no. 3 (February 8, 2018): 797–808. http://dx.doi.org/10.5194/bg-15-797-2018.

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Abstract. Mosses, as major components of later successional biological soil crusts (biocrusts), play many critical roles in arid and semiarid ecosystems. Recently, some species of desiccation-tolerant mosses have been artificially cultured with the aim of accelerating the recovery of biocrusts. Revealing the factors that influence the vegetative propagation of mosses, which is an important reproductive mode of mosses in dry habitats, will benefit the restoration of moss crusts. In this study, three air-dried desiccation-tolerant mosses (Barbula unguiculata, Didymodon vinealis, and Didymodon tectorum) were hermetically sealed and stored at five temperature levels (0, 4, 17, 25, and 30 °C) for 40 days. Then, the vegetative propagation and physiological characteristics of the three mosses were investigated to determine the influence of storage temperature on the vegetative propagation of desiccation-tolerant mosses and the mechanism. The results showed that the vegetative propagation of the three mosses varied with temperature. The most variation in vegetative propagation among storage temperatures was observed in D. tectorum, followed by the variation observed in B. unguiculata. In contrast, no significant difference in propagation among temperatures was found in D. vinealis. The regenerative capacity of the three mosses increased with increasing temperature from 0 to 17 °C, accompanied by a decrease in malondialdehyde (MDA) content, and decreased thereafter. As the temperature increased, the chlorophyll and soluble protein contents increased in B. unguiculata but decreased in D. vinealis and D. tectorum. As to storage, the MDA and soluble sugar contents increased after storage. The MDA content of the three mosses increased at each of the investigated temperatures by more than 50 % from the initial values, and the soluble sugar content became higher than before in the three mosses. The integrity of cells and cell membranes is likely the most important factor influencing the vegetative propagation of desiccation-tolerant mosses. A 40-day storage period caused cell injury. Our results suggest that storage temperature can enhance or suppress such injury and change the regenerative capacity of the three mosses. The data indicate that the suitable storage temperature is 4 °C for B. unguiculata and 17 °C for both D. vinealis and D. tectorum.
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34

Weldearegay, D. F., F. Yan, S. K. Rasmussen, S. E. Jacobsen, and F. Liu. "Physiological response cascade of spring wheat to soil warming and drought." Crop and Pasture Science 67, no. 5 (2016): 480. http://dx.doi.org/10.1071/cp15211.

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Climate change is affecting wheat production in Northern Europe; in particular, drought and soil warming during anthesis may cause significant yield losses of the crop. In a search for genotypes tolerant to these stresses, the physiological responses of three spring wheat cultivars to increased soil temperature (3°C above normal) (H), drought (D) and their combination (HD) were investigated. The plants were grown in pots in a climate-controlled greenhouse. Stomatal conductance (gs), photosynthesis (A), leaf water potential (Ψl), and relative water content (RWC) were measured during the treatment period. The responses of these variables to soil drying (for both D and HD) were described by a linear–plateau model, indicating the soil water thresholds at which the variables started to decrease in relation to the control plants. The H treatment alone hardly affected the variables, whereas both D and HD had significant effects. The variable most sensitive to soil drying was gs, followed by A, Ψl, and RWC. Among the three cultivars, earlier stomatal closure during drought in Alora could be a good adaptive strategy to conserve soil water for a prolonged drought, but may not be of benefit under intermittent drought conditions. Later stomatal closure and decline in A for Scirocco under HD and D stresses would be a favourable trait to sustain productivity under intermittent drought. A lower soil-water threshold of gs associated with a later decrease in A for Scirocco implies that the cultivar was less susceptible to HD and D stresses at anthesis.
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Costa, Luisa Dalla, Nicola Tomasi, Stefano Gottardi, Francesco Iacuzzo, Giovanni Cortella, Lara Manzocco, Roberto Pinton, Tanja Mimmo, and Stefano Cesco. "The Effect of Growth Medium Temperature on Corn Salad [Valerianella locusta (L.) Laterr] Baby Leaf Yield and Quality." HortScience 46, no. 12 (December 2011): 1619–25. http://dx.doi.org/10.21273/hortsci.46.12.1619.

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Soil temperature has a crucial impact on physiological processes and growth of plants with important consequences for plant productivity and food safety including nitrate accumulation in leaf blades of leaf vegetables. Consumer demand for high-quality, fresh-cut vegetables has increased rapidly in the last decades, and temperature modulation can help control nitrate concentration in fresh vegetables, an important trait of product safety. Corn salad plants [Valerianella locusta (L.) Laterr., cultivar Gala] were grown at three root temperatures (15, 20, and 25 °C) in a floating system. This experimental setup allowed to directly evaluate the effect of root temperature on yield and plant quality excluding the effect on soil processes and properties. Nutrient solution was renewed weekly and kept aerated while air temperature was maintained constant at 20 °C for all treatments during the entire time of experiments. At harvest, plants were collected, the shelf life evaluated, and the nutrient uptake [NO3−, iron (Fe) from 59Fe-o,oEDDHA, and 35SO42−] and mineral content were determined. Results showed that growing conditions at 20 °C of the nutrient solution led to the best plant performance in terms of yield, nitrate content at leaf level, root biomass, leaf area, and greenness with positive effects on postharvest quality, i.e., less rapid leaf loss of greenness and leaf fresh weight (FW) loss during conservation at 4 °C. At this temperature condition of the nutrient solution, it has also been observed an enhanced functionality of mechanisms involved in the acquisition of nutrients like NO3−, Fe, and SO42−, which are known to play an important role in nitrate level in leaf tissues of crops. Plants grown at 15 °C showed minor growth, whereas the nutrient solution at 25 °C caused stress for the plants affecting negatively the quality and yield. Overall, the results obtained showed that root temperature plays a fundamental role in several plant processes that affect yield and its quality; for hydroponic system cultivations, a level of growing-medium temperature close to that of the surrounding air seems suitable.
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Dorairaj, Deivaseeno, Muhammad Fahmi Suradi, Nursyamimi Syafiqah Mansor, and Normaniza Osman. "Root architecture, rooting profiles and physiological responses of potential slope plants grown on acidic soil." PeerJ 8 (August 24, 2020): e9595. http://dx.doi.org/10.7717/peerj.9595.

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Globally, there has been an increase in the frequency of landslides which is the result of slope failures. The combination of high intensity rainfall and high temperature resulted in the formation of acidic soil which is detrimental to the healthy growth of plants. Proper plant coverage on slopes is a prerequisite to mitigate and rehabilitate the soil. However, not all plant species are able to grow in marginal land. Thus, this study was undertaken to find a suitable slope plant species. We aimed to evaluate the effect of different soil pH on root profiles and growth of three different potential slope plant species namely, Melastoma malabathricum, Hibiscus rosa-sinensis and Syzygium campanulatum. M. malabathricum showed the highest tolerance to acidic soil as it recorded the highest plant height and photosynthetic rate. The root systems of M. malabathricum, H. rosa-sinensis and S. campanulatum were identified as M, VH- and R-types, respectively. The study proposed M. malabathricum which possessed dense and shallow roots to be planted at the toe or top of the slope while H. rosa-sinensis and S. campanulatum to be planted in the middle of a slope. S. campanulatum consistently recorded high root length and root length density across all three types of soil pH while M. malabathricum showed progressive increase in length as the soil pH increased. The root average diameter and root volume of M. malabathricum outperformed the other two plant species irrespective of soil pH. In terms of biomass, M. malabathricum exhibited the highest root and shoot dry weights followed by S. campanulatum. Thus, we propose M. malabathricum to be planted on slopes as a form of soil rehabilitation. The plant species displayed denser rooting, hence a stronger root anchorage that can hold the soil particles together which will be beneficial for slope stabilization.
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37

Smith, D. L., J. E. Hollowell, T. G. Isleib, and B. B. Shew. "Analysis of Factors That Influence the Epidemiology of Sclerotinia minor on Peanut." Plant Disease 90, no. 11 (November 2006): 1425–32. http://dx.doi.org/10.1094/pd-90-1425.

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In North Carolina, sclerotia of Sclerotinia minor germinate myceliogenically to initiate infections on peanut. The effects of soil temperature and soil matric potential (ψM on germination and growth of S. minor have not been well characterized, and little is known about relative physiological resistance in different parts of the peanut plant. Laboratory tests examined the ability of the fungus to germinate, grow, and infect detached peanut leaflets at soil temperatures ranging from 18 to 30°C at ψM of -100, -10, and -7.2 kPa. In addition, detached pegs, leaves, main stems, and lateral branches from three peanut lines varying in field resistance were examined for resistance to infection by S. minor. Sclerotial germination was greatest at 30°C and ψM of -7.2 kPa. Final mycelial diameters decreased with decreasing ψM, whereas soil matric potential did not affect lesion development. Mycelial growth and leaflet lesion expansion were maximal at 18 or 22°C. Soil ψM did not affect leaflet infection and lesion expansion. Lesions were not observed on leaves incubated at temperatures of 29°C or above, but developed when temperatures were reduced to 18 or 22°C 2 days after inoculation. Pegs and leaflets were equally susceptible to infection and were more susceptible than either main stems or lateral branches. Results of this work, particularly the effects of temperature on S. minor, and knowledge of peanut part susceptibility has application in improving Sclerotinia blight prediction models for recommending protective fungicide applications.
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38

Yu, Dapao, Qingwei Wang, Xiaoyu Wang, Limin Dai, and Maihe Li. "Microsite Effects on Physiological Performance of Betula ermanii at and Beyond an Alpine Treeline Site on Changbai Mountain in Northeast China." Forests 10, no. 5 (May 9, 2019): 400. http://dx.doi.org/10.3390/f10050400.

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The alpine treeline demarcates the temperature-limited upper elevational boundary of the tree life form. However, this treeline does not always occur exclusively as a sharp “line”, outposts of tree groups (OTG) with a height of at least 3 m are often observed in microsites up to several hundred meters beyond the line of continuous forest on some mountains. This suggests that other factors such as microenvironment may play a significant role in compensating for the alpine tree facing growth-limiting low temperature conditions. To test the microenvironment effects, this study compared the differences in growing conditions (climate and soil properties) and ecophysiological performance of Erman’s birch (Betula ermanii Cham.) trees growing in a continuous treeline site (CTL, ~1950 m above sea level, a.s.l.) and OTGs (~2050 m a.s.l.) on Changbai Mountain in northeastern China. The results show the average 2-m air temperature for OTG was slightly lower in the non-growing season than which at the CTL (−10.2 °C < −8.4 °C), there was no difference in growing season air temperature and soil temperature at 10 cm depth between CTL and OTG. The contents of focal soil nutrients in CTL and OTG were similar. Difference in K and Mn contents between sites were detected in leaves, difference in K, Mn, and Zn in shoots. However, comparing similarity of ecophysiological performances at an individual level, trees at CTL and OTG show no significant difference. Our study reveals that mature trees at the CTL and OTG experience generally similar environmental conditions (climate and soil properties) and exhibit similar overall ecophysiological performance (reflected in carbon reserves and nutrients). This might provide insight into how mature trees might be able to survive in areas higher than the continuous treeline, as well as the importance of microclimatic amelioration provided by protective microsites and the trees themselves.
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39

Bonfante, A., A. Agrillo, R. Albrizio, A. Basile, R. Buonomo, R. De Mascellis, A. Gambuti, et al. "Functional homogeneous zones (fHZs) in viticultural zoning procedure: an Italian case study on Aglianico vine." SOIL 1, no. 1 (June 9, 2015): 427–41. http://dx.doi.org/10.5194/soil-1-427-2015.

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Abstract. This paper aims to test a new physically oriented approach to viticulture zoning at farm scale that is strongly rooted in hydropedology and aims to achieve a better use of environmental features with respect to plant requirements and wine production. The physics of our approach are defined by the use of soil–plant–atmosphere simulation models, applying physically based equations to describe the soil hydrological processes and solve soil–plant water status. This study (part of the ZOVISA project) was conducted on a farm devoted to production of high-quality wines (Aglianico DOC), located in southern Italy (Campania region, Mirabella Eclano, AV). The soil spatial distribution was obtained after standard soil survey informed by geophysical survey. Two homogeneous zones (HZs) were identified; in each one a physically based model was applied to solve the soil water balance and estimate the soil functional behaviour (crop water stress index, CWSI) defining the functional homogeneous zones (fHZs). For the second process, experimental plots were established and monitored for investigating soil–plant water status, crop development (biometric and physiological parameters) and daily climate variables (temperature, solar radiation, rainfall, wind). The effects of crop water status on crop response over must and wine quality were then evaluated in the fHZs. This was performed by comparing crop water stress with (i) crop physiological measurement (leaf gas exchange, chlorophyll a fluorescence, leaf water potential, chlorophyll content, leaf area index (LAI) measurement), (ii) grape bunches measurements (berry weight, sugar content, titratable acidity, etc.) and (iii) wine quality (aromatic response). This experiment proved the usefulness of the physically based approach, also in the case of mapping viticulture microzoning.
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40

Bulanov, Alexander G., Anton A. Shagaev, Alexey A. Belov, and Nikolay S. Markvichev. "Physiological properties of resistance strain Fusarium oxysporum." Butlerov Communications 57, no. 2 (February 28, 2019): 144–50. http://dx.doi.org/10.37952/roi-jbc-01/19-57-2-144.

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Soil microorganisms have a significant effect on microbiological cenosis. Such communities are called the root micro-flora of the plant. All organisms in the microflora are divided into two groups: rhizoplane, living directly on the root system of the plant, and rhizosphere, developing in the root area of the plant. The intensive population of the root and root zones of a plant is primarily associated with the release (exosmosome) of organic substances or exudates formed during the life of the plant. The plant microflora includes not only symbiotic and commensalithic types of interaction, but also parasitic forms. Parasitic pathogens are divided into two groups: pathogenic and conditionally pathogenic. Conditionally pathogenic microorganisms are in a latent state and have an effect only when certain conditions arise for their activation and development. Such conditions may include changes in temperature, humidity, disturbance of plant homeostasis, or damage to the integument. Pathogenic microorganisms, by contrast, are always active and infect the host organism upon contact. The pathogenicity of a microorganism is a complex of traits that adversely affect the health of the plant, cause various pathologies, leading to growth inhibition and partial inhibition of the development or complete destruction of the culture. The pathogenic properties of the Fusarium oxysporum culture F201 were investigated against the cucumber culture by Atlet F1. It was shown the phytopathogenic microorganisms Fusarium oxysporum has acquired signs of resistance without lost her aggressiveness properties against a plant. Fusarium oxysporum quite comparable with the common strain.
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41

Yang, Jun. "EFFECTS OF HIGH TEMPERATURE ON YIELD, QUALITY AND PHYSIOLOGICAL COMPONENTS OF EARLY RICE." Pakistan Journal of Agricultural Sciences 55, no. 01 (March 1, 2018): 13–22. http://dx.doi.org/10.21162/pakjas/18.2621.

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42

Soto, W., J. Gutierrez, M. D. Remmenga, and M. K. Nishiguchi. "Salinity and Temperature Effects on Physiological Responses of Vibrio fischeri from Diverse Ecological Niches." Microbial Ecology 57, no. 1 (June 28, 2008): 140–50. http://dx.doi.org/10.1007/s00248-008-9412-9.

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43

Forge, T. A., and A. E. MacGuidwin. "Effects of water potential and temperature on survival of the nematode Meloidogyne hapla in frozen soil." Canadian Journal of Zoology 70, no. 8 (August 1, 1992): 1553–60. http://dx.doi.org/10.1139/z92-214.

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The effects of water potential on the survival of Meloidogyne hapla second-stage juveniles in frozen soil were studied. Soil-water potentials and freezing temperatures tested ranged from 0 to −1910 kPa and from −1 to −4 °C, respectively. Survivorship was greater in soil at water potentials of −1910 to −520 kPa than in soil at higher water potentials. Saturating the soil immediately before freezing reduced survivorship, but it was still greater for juveniles exposed to low water potentials before saturation. Thus, low water potentials increased survivorship directly by reducing the pore space filled with ice and indirectly by causing physiological changes that increased the ability of juveniles to survive frozen conditions. Exposure to low water potentials in polyethylene glycol solutions at 24 °C also caused an increase in the percentage of juveniles that survived frozen conditions. Regardless of water potential experienced (−2050 to 0 kPa), subsequent exposure to 4 °C caused an additional increase in the percentage surviving frozen conditions. However, exposure to a low water potential (−1110 kPa) at 0 °C did not cause an increase in tolerance of frozen conditions. Juveniles extracted from frozen soil or solutions appeared desiccated. Survivorship in frozen solutions was compared with survivorship at water potential and temperature combinations equivalent to those of the frozen solutions. Survivorship was the same in both situations, indicating that desiccation may be an important stress responsible for mortality in frozen conditions.
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44

Liu, Xiaozhong, and Bingru Huang. "Mowing Height Effects on Summer Turf Growth and Physiological Activities for Two Creeping Bentgrass Cultivars." HortScience 38, no. 3 (June 2003): 444–48. http://dx.doi.org/10.21273/hortsci.38.3.444.

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Low mowing increases ball roll distance on putting greens, but may affect growth and physiological responses to summer heat stress. The objective of this study was to examine whether the effect of mowing heights on turf summer performance was associated with changes in photosynthetic activities and respiration rate for two creeping bentgrass [Agrostis palustris (L.) Huds] cultivars, `Crenshaw' and `Penncross'. Both cultivars were grown under USGA-specification putting green conditions from 1997 to 1998. Grasses were mowed daily at a 3-mm (low mowing) or 4-mm (high mowing) height. Turf quality, net photosynthesis rate (Pn), and leaf photochemical efficiency (Fv/Fm) declined, whereas respiration rate of whole plants, canopy minus air temperature, and soil temperatures increased under low mowing compared to those at the high mowing height. The decline or increase in those parameters under low mowing was more pronounced in summer than in spring or fall months. The results showed that turf quality was better at the 4-mm mowing height, especially during summer months. Better quality at the higher mowing height could be related to the maintenance of higher photosynthetic activities and lower respiration rate. Mowing at the lower height had more adverse effects on turf growth and photosynthetic capacity for `Penncross' than `Crenshaw', particularly during summer months.
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45

Bello, Iliya A., Harlene Hatterman-Valenti, and Micheal D. K. Owen. "Effects of stratification, temperature, and oxygen on woolly cupgrass (Eriochloa villosa) seed dormancy." Weed Science 46, no. 5 (October 1998): 526–29. http://dx.doi.org/10.1017/s0043174500091037.

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Laboratory and field experiments were conducted to determine the effects of temperature, oxygen, and seed burial depth on woolly cupgrass seed dormancy. Woolly cupgrass seeds at physiological maturity are innately dormant. Stratifying these seeds at 5 C for 8 wk increased germination to greater than 90%, regardless of the germination temperature. Alternating the germination temperature hastened germination by 2 wk, while seedcoat removal hastened germination by another 4 wk. Low oxygen concentration was more detrimental to seed germination when seeds were subjected to constant temperatures in comparison to alternating temperatures. Oxygen concentration did not affect seed germination when seeds were placed in an alternating 15/25 C regime. Seed germination was approximately 15% less when seeds were stratified for 2 wk and subjected to oxygen concentrations below the ambient oxygen concentration, whereas this decrease was present only at the 8% oxygen concentration when seeds were stratified 4 wk. In the field, the stratification requirement was satisfied by early December for the study year. Seeds remaining on the soil surface overcame dormancy sooner than seeds buried 2 or 4 cm.
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46

Maaswinkel, R. H. M., and G. W. H. Welles. "Factors influencing glassiness in lettuce." Netherlands Journal of Agricultural Science 34, no. 1 (February 1, 1986): 57–65. http://dx.doi.org/10.18174/njas.v34i1.16816.

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Glassiness, a physiological disorder occurring in greenhouses between November and February under low natural light conditions, is probably due to an imbalance between water uptake and transpiration. Eight greenhouse experiments were carried out during winter. Experiments with soil-grown plants showed that soil compaction and nitrogen supply were negatively correlated with the amount of glassiness whereas the amount of nightly outgoing radiation (from inside the greenhouse) was positively correlated with glassiness. Studies using the nutrient film technique showed that the positive effect of nitrogen was probably due to higher salt concentration rather than a specific effect of NO3-. Clear cultivar differences were observed with Dalida, Mistra and Rolinda showing less glassiness than Renate, Amanda Plus and Decisco. High soil compaction, high plant temperature, tolerant cultivars and higher total salt concentration could all be used to reduce glassiness. (Abstract retrieved from CAB Abstracts by CABI’s permission)
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47

NAKATANI, Makoto, Atsushi OYANAGI, and Yasushi WATANABE. "Effects of soil temperatures on the rooting of cut-sprouts of sweet potato (Ipomoea batatas Lam.). I. Optimum soil temperature for rooting and effects of high soil temperatures on the physiological and anatomical characteristics of roots." Japanese journal of crop science 55, no. 2 (1986): 208–16. http://dx.doi.org/10.1626/jcs.55.208.

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48

Singh, Jagdish, and M. S. Hadda. "Phenology and thermal indices of maize (Zea mays L.) influenced by subsoil compaction and nitrogen fertilization under semi-arid irrigated conditions." Journal of Applied and Natural Science 6, no. 2 (December 1, 2014): 349–55. http://dx.doi.org/10.31018/jans.v6i2.426.

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The magnitude of yield reduction due to soil compaction is variable and depends on the soil type, fertility status and other soil and environmental factors. The present investigation was carried out at the research farm, Department of Soil Science, Punjab Agricultural University, Ludhiana. The experiment was conducted to evaluate the effect of different levels of subsoil compaction and nitrogen fertilization on maize phenology, yield and heat use efficiency. The C2 (subsoil bulk density (Db)= >1.8 Mg m-3) treatment reduced yield by 15.5 and 24.3 % and heat use efficiency (HUE) by 15.2 and 20.9 % than that in C0 (subsoil Db=1.55-1.65 Mg m-3) treatment during the year 2012 and 2013, respectively. The tasseling and silking stage was delayed, while physiological maturity was advanced under C2 subsoil compaction treatment than that in C0 treatment. The N2 treatment improved the yield by 14.9 and 13.9 % and HUE by 15.2 and 14.3 % than that in N0 treatment during the year 2012 and 2013, respectively. Maize took more days to reach physiological maturity under N2 treatment as compared to N0 treatment. Phenothermal index (PTI) showed that crop reached different stages earlier under C1 and C2 than that of C0. The data emphasized the need to take care of soil strength and soil temperature related parameters along with weather conditions for better yield prediction using thermal time.
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49

Kaka, H., P. A. Opute, and M. S. Maboeta. "Potential Impacts of Climate Change on the Toxicity of Pesticides towards Earthworms." Journal of Toxicology 2021 (August 20, 2021): 1–14. http://dx.doi.org/10.1155/2021/8527991.

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This review examined one of the effects of climate change that has only recently received attention, i.e., climate change impacts on the distribution and toxicity of chemical contaminants in the environment. As ecosystem engineers, earthworms are potentially threatened by the increasing use of pesticides. Increases in temperature, precipitation regime changes, and related extreme climate events can potentially affect pesticide toxicity. This review of original research articles, reviews, and governmental and intergovernmental reports focused on the interactions between toxicants and environmental parameters. The latter included temperature, moisture, acidification, hypoxia, soil carbon cycle, and soil dynamics, as altered by climate change. Dynamic interactions between climate change and contaminants can be particularly problematic for organisms since organisms have an upper and lower physiological range, resulting in impacts on their acclimatization capacity. Climate change variables such as temperature and soil moisture also have an impact on acidification. An increase in temperature will impact precipitation which might impact soil pH. Also, an increase in precipitation can result in flooding which can reduce the population of earthworms by not giving juvenile earthworms enough time to develop into reproductive adults. As an independent stressor, hypoxia can affect soil organisms, alter bioavailability, and increase the toxicity of chemicals in some cases. Climate change variables, especially temperature and soil moisture, significantly affect the bioavailability of pesticides in the soil and the growth and reproduction of earthworm species.
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50

Lo, Miranda, Dieter M. Bulach, David R. Powell, David A. Haake, James Matsunaga, Michael L. Paustian, Richard L. Zuerner, and Ben Adler. "Effects of Temperature on Gene Expression Patterns in Leptospira interrogans Serovar Lai as Assessed by Whole-Genome Microarrays." Infection and Immunity 74, no. 10 (October 2006): 5848–59. http://dx.doi.org/10.1128/iai.00755-06.

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ABSTRACT Leptospirosis is an important zoonosis of worldwide distribution. Humans become infected via exposure to pathogenic Leptospira spp. from infected animals or contaminated water or soil. The availability of genome sequences for Leptospira interrogans, serovars Lai and Copenhageni, has opened up opportunities to examine global transcription profiles using microarray technology. Temperature is a key environmental factor known to affect leptospiral protein expression. Leptospira spp. can grow in artificial media at a range of temperatures reflecting conditions found in the environment and the mammalian host. Therefore, transcriptional changes were compared between cultures grown at 20°C, 30°C, 37°C, and 39°C to represent ambient temperatures in the environment, growth under laboratory conditions, and temperatures in healthy and febrile hosts. Data from direct pairwise comparisons of the four temperatures were consolidated to examine transcriptional changes at two generalized biological conditions representing mammalian physiological temperatures (37°C and 39°C) versus environmental temperatures (20°C and 30°C). Additionally, cultures grown at 30°C then shifted overnight to 37°C were compared with those grown long-term at 30°C and 37°C to identify genes potentially expressed in the early stages of infection. Comparison of data sets from physiological versus environmental experiments with upshift experiments provided novel insights into possible transcriptional changes at different stages of infection. Changes included differential expression of chemotaxis and motility genes, signal transduction systems, and genes encoding proteins involved in alteration of the outer membrane. These findings indicate that temperature is an important factor regulating expression of proteins that facilitate invasion and establishment of disease.
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