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Journal articles on the topic 'Sol-Gel/Centrifugation'

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1

WICKSTRÖM, Claes, Cecilia CHRISTERSSON, Julia R. DAVIES, and Ingemar CARLSTEDT. "Macromolecular organization of saliva: identification of ‘insoluble’ MUC5B assemblies and non-mucin proteins in the gel phase." Biochemical Journal 351, no. 2 (2000): 421–28. http://dx.doi.org/10.1042/bj3510421.

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Stimulated human submandibular/sublingual (HSMSL) and whole saliva were separated into sol and gel phases and mucins were isolated by density-gradient centrifugation in CsCl/4M guanidinium chloride. MUC5B and MUC7 were identified using anti-peptide antisera raised against sequences within the MUC5B and MUC7 apoproteins respectively. MUC7 was found mainly in the sol phase of both HSMSL and whole saliva, but some MUC7 was consistently present in the gel phase, suggesting that this mucin may interact with the salivary gel matrix. In HSMSL saliva, MUC5B was found in the gel phase; however, most of
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2

DAVIES, Julia R., Naila SVITACHEVA, Louise LANNEFORS, Ragnhild KORNFÄLT, and Ingemar CARLSTEDT. "Identification of MUC5B, MUC5AC and small amounts of MUC2 mucins in cystic fibrosis airway secretions." Biochemical Journal 344, no. 2 (1999): 321–30. http://dx.doi.org/10.1042/bj3440321.

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To investigate the genetic identities of the mucins secreted in cystic fibrosis (CF) airways, sputum was collected from five individuals. Samples were separated into gel and sol phases by high-speed centrifugation and the gel phase was extracted in 6 M guanidinium chloride. The ‘insoluble’ residue remaining after extraction of the gel phase was brought into solution by reduction/alkylation. Density-gradient centrifugation in CsCl revealed polydisperse distributions of sialic acid-containing mucins in the gel phase, insoluble residue and sol phase fractions and the degree of variation between t
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3

HOVENBERG, Hans W., Ingemar CARLSTEDT, and Julia R. DAVIES. "Mucus glycoproteins in bovine trachea: identification of the major mucin populations in respiratory secretions and investigation of their tissue origins." Biochemical Journal 321, no. 1 (1997): 117–24. http://dx.doi.org/10.1042/bj3210117.

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Bovine respiratory secretions were separated into gel and sol phases to allow the identification of the gel-forming mucins. Mucins were subsequently isolated from the surface epithelium and submucosal tissue to investigate the tissue origins of the species in the secretions. Density-gradient centrifugation revealed ‘high-density’ and ‘low-density’ mucins in the gel phase of the secretions. The ‘high-density’ mucins were large, composed of subunits joined by disulphide bonds and contained two highly glycosylated domains of apparently different lengths, whereas the ‘low-density’ mucins were smal
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4

Thornton, D. J., J. K. Sheehan, H. Lindgren, and I. Carlstedt. "Mucus glycoproteins from cystic fibrotic sputum. Macromolecular properties and structural ‘architecture’." Biochemical Journal 276, no. 3 (1991): 667–75. http://dx.doi.org/10.1042/bj2760667.

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Mucus glycoproteins (mucins) were isolated from sputum of patients with cystic fibrosis (CF) after separation into sol and gel phases. The mucus gel was solubilized with gentle stirring in 6 M-guanidinium chloride supplemented with proteinase inhibitors, and purification of mucins was subsequently achieved by isopycnic density-gradient centrifugation in CsCl/guanidinium chloride. Density-gradient centrifugation also revealed a heterogeneity of the macromolecules, the pattern of which varied between individuals, and mucins from the gel phase was pooled as ‘heavy’ and ‘light’ fractions. Gel chro
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5

Alias, S. S., A. B. Ismail, and A. A. Mohamad. "Effect of pH on ZnO nanoparticle properties synthesized by sol–gel centrifugation." Journal of Alloys and Compounds 499, no. 2 (2010): 231–37. http://dx.doi.org/10.1016/j.jallcom.2010.03.174.

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6

Davies, J. R., J. T. Gallagher, P. S. Richardson, J. K. Sheehan, and I. Carlstedt. "Mucins in cat airway secretions." Biochemical Journal 275, no. 3 (1991): 663–69. http://dx.doi.org/10.1042/bj2750663.

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Mucous secretions were obtained from cat tracheas that had received [3H]glucose and [35S]sulphate to radiolabel mucus glycoproteins biosynthetically. Samples were collected under resting (‘basal’) conditions as well as after pilocarpine stimulation and were separated into gel and sol phases by centrifugation. Macromolecules were partially purified by using gel chromatography on Sepharose CL-4B, and the species that were eluted with the void volume were then separated into two major populations with isopycnic density-gradient centrifugation in CsCl. The major component from the gel phase of pil
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7

Hu, Yu Long, Xiao Dong Zhang, Hong Fang Liu, and Xing Peng Guo. "High-Efficiency Preparation of N-Doped Titania with High Visible Light Photocatalytic Activity Using Composite N Precursor." Key Engineering Materials 645-646 (May 2015): 368–74. http://dx.doi.org/10.4028/www.scientific.net/kem.645-646.368.

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N-doped TiO2 nanoparticle powders were prepared efficiently by the sol-gel method using triethylamine and ammonium hydroxide as composite N precursor. The as-prepared N-doped TiO2 precursor powders were calcined at 300°C in air for 3 h and subsequently annealed at 300°C in air for 2.5 h. The samples were characterized by X-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, thermo-gravimetric analysis, and X-ray photoelectron spectroscopy. The visible light photocatalytic activities of as-prepared samples were evaluated by photodecomposition of methyl or
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8

Pollert, E., O. Kaman, P. Veverka, et al. "Core–shell La 1− x Sr x MnO 3 nanoparticles as colloidal mediators for magnetic fluid hyperthermia." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 368, no. 1927 (2010): 4389–405. http://dx.doi.org/10.1098/rsta.2010.0123.

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Core–shell nanoparticles consisting of La 0.75 Sr 0.25 MnO 3 cores covered by silica were synthesized by a procedure consisting of several steps, including the sol–gel method in the presence of citric acid and ethylene glycol, thermal and mechanical treatment, encapsulation employing tetraethoxysilane and final separation by centrifugation in order to get the required size fraction. Morphological studies revealed well-separated particles that form a stable water suspension. Magnetic studies include magnetization measurements and investigation of the ferromagnetic–superparamagnetic–paramagnetic
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9

Hu, Xufeng, Han Sun, Xiaopeng Zhao, and Jianbo Yin. "Enhanced Electrorheological Polishing Efficiency of Alumina-Doped Titanium Dioxide Particles." Materials 16, no. 6 (2023): 2347. http://dx.doi.org/10.3390/ma16062347.

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Electrorheological (ER) polishing is a novel polishing technology having flexible and tunable characteristics. At present, ER polishing uses ER particles to drive abrasive particles to polish the material surface. Under the action of high-speed centrifugation, the abrasive particles are easily separated from ER particles due to their significantly different ER effect, and this can easily cause the degradation of polishing ability. In this work, alumina-doped titanium dioxide ER polishing particles were developed via a sol-gel method. As a classical abrasive, alumina has higher hardness and can
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10

Rembeza, E. S., T. V. Svistova, N. N. Kosheleva, et al. "Synthesis and Electrophysical Properties of Copper Oxide Films for Gas Sensors." Nano- i Mikrosistemnaya Tehnika 25, no. 2 (2023): 61–68. http://dx.doi.org/10.17587/nmst.25.61-68.

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Thin films of copper oxide were fabricated by sol-gel method. For the preparation of CuO thin films, a sol-gel method based on copper acetate (Cu(CH3COO)2•H2O), isopropyl alcohol (C3H8O) and diethylamine ((CH3CH2 )2NH) was used. The film was applied by centrifugation at a speed of 1500 rpm for 75 seconds. The films were applied in 2, 4, 8 layers with drying between layers for 10 minutes at 250 °C. The thermal probe method was used to determine the p-type of conductivity of the films. The films were annealed at temperatures of 300, 400, 500, 600 °C. By method of X-ray phase analysis, it was fou
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11

Ratirotjanakul, Waranya, Tanapon Sioloetwong, Teeraporn Suteewong, and Pramuan Tangboriboonrat. "Green Synthesis of AgNPs Coated Mesoporous Silica Nanoparticles Using Tyrosine as Reducing/Stabilising Agent." Materials Science Forum 928 (August 2018): 89–93. http://dx.doi.org/10.4028/www.scientific.net/msf.928.89.

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A novel, simple and environmental friendly approach to fabricate silver nanoparticles (AgNPs) on mesoporous silica nanoparticles (MSNs) using tyrosine (Tyr) as biological reducing agent was developed. The functionalization of Tyr with MSNs (Tyr-MSNs) (150 nm in length) by the sol-gel process was confirmed by the characteristic peaks of amino, carboxyl and silanol groups appeared in FTIR spectrum and the change of the zeta potential from 0 mV at pH 2 to-60 mV at pH 12. Then, AgNPs were formed on the surface of Tyr-MSNs (Tyr-MSN@AgNPs) via only reducibility from phenolic group of Tyr and catalyt
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12

Cui, Suihan, Qingdong Ruan, Cuiqing Jiang, et al. "Pre-lithiated Li(NixMnyCoz)O2 nanoparticles with a double-layer lithium structure." Functional Materials Letters 13, no. 04 (2020): 2050020. http://dx.doi.org/10.1142/s1793604720500204.

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Li(NixMnyCoz)O2 cathode materials (NMC) have advantages such as the good Li ion diffusivity, stable reversible capacity, and environmental compatibility in spite of a low actual capacity. Although a double-layer lithium structure can be generated by pre-lithiation, the thickness is very small and the capacity improvement is limited. In this work, a series of Li([Formula: see text][Formula: see text][Formula: see text])O2 nanoparticles are prepared by the sol–gel method and centrifugation and the pre-lithiation process are monitored by transmission electron microscope (TEM). A double lithium st
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13

Callone, Emanuela, Giovanni Carturan, Marco Ischia, and Adriana Sicurelli. "Nanometric oxides from molecular precursors in the presence of starch: Coatings of glass with these oxides in silica sols." Journal of Materials Research 21, no. 7 (2006): 1726–37. http://dx.doi.org/10.1557/jmr.2006.0217.

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Sn, Sn-Sb, Ti, Ce, Zr, Zn, In, In–Sn, and Fe oxides of nanometric size were obtained through the hydrolytic route, starting from molecular precursors in the presence of starch. Gels were treated with α-amylase to produce oxide suspensions and starch degradation. Solid oxide products resulted from reaction with H2O2, washing with H2O, and centrifugation of the suspensions. The nanometric size and morphology of crystallites were assessed by x-ray diffractometry, transmission electron microscopy, and, for SnO2, solid-state nuclear magnetic resonance. Product consolidation at 600 °C did not produc
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14

Garzón-Agudelo, P. A., and J. Bautista-Ruiz. "Effect of layer thickness on anticorrosive performance of 316L steel coated with bismuth-titanium films." Journal of Physics: Conference Series 2139, no. 1 (2021): 012014. http://dx.doi.org/10.1088/1742-6596/2139/1/012014.

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Abstract Bismuth and titanium are elements with remarkable properties and applications in technological developments and in the field of biomedical engineering. The sol-gel method was used to form a bismuth-titanium system, which allowed to establish if it was possible to obtain films with anticorrosive properties on 316L stainless steel. The anticorrosive response was evaluated by means of Tafel curves, defining the parameters to obtain thin and functional films with good tribological properties. The coatings were obtained by the spin coating technique, varying the spin speeds from 3000 rpm t
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15

Redha, Latrous Ahmed, Touafek Naima, and Mahamdi Ramdane. "Elaboration by spin coating and characterization of CZTS films." All Sciences Abstracts 1, no. 1 (2023): 4. http://dx.doi.org/10.59287/as-abstracts.533.

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Thin films of Cu2ZnSnS4 (CZTS) were deposited by the sol-gel spin coating method from a non-aqueous solution containing different molarities of precursors, cupric chloride CuCl2 (2H2O) (2M), zinc chloride ZnCl2 (1M), stannic chloride SnCl2 (2H2O) (1M) and thiourea SC(NH2)2 (8M) dissolved in different volumes of solvents, methanol CH3OH and deionized water. A light-yellow solution was formed by thermal centrifugation at adjustable speeds for several minutes. Characterizations were carried out after deposition, drying and thermal annealing of these films. XRD spectra showed the formation of seve
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16

Xekoukoulotakis, N. P., D. Mantzavinos, R. Dillert, and D. Bahnemann. "Synthesis and photocatalytic activity of boron-doped TiO2 in aqueous suspensions under UV-A irradiation." Water Science and Technology 61, no. 10 (2010): 2501–6. http://dx.doi.org/10.2166/wst.2010.150.

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Boron-doped TiO2 photocatalysts were synthesized employing a sol-gel method. Boric acid was used as the boron source and titanium tetra-isopropoxide as the TiO2 precursor, both dissolved in isopropanol. Nominal boron to titanium atomic ratios were in the range 0 to 4%. After the hydrolysis step, two different procedures for the recovery of TiO2 were followed, based on either centrifugation of the resulting reaction mixture or evaporation of the solvent under reduced pressure, both followed by a subsequent calcination step performed at 400 or 500°C. The photocatalytic efficiency of the synthesi
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17

Kobayashi, Yoshio, Hikaru Morimoto, Tomohiko Nakagawa, Yohsuke Kubota, Kohsuke Gonda, and Noriaki Ohuchi. "Preparation of Gd Complex-Immobilized Silica Particles and Their Application to MRI." ISRN Nanotechnology 2013 (April 10, 2013): 1–6. http://dx.doi.org/10.1155/2013/908614.

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A preparation method for Gd-ethylenediaminetetraacetic acid disodium salt dihydrate (ETDA) complex-immobilized silica particles (Gd-EDTA/SiO2) is proposed. Preparation of spherical silica particles was performed by a sol-gel method at 35°C using 0.2 M tetraethylorthosilicate, 25 M H2O, and 0.01 M NaOH in ethanol, which produced silica particles with an average size of nm. Immobilization of Gd-EDTA on the silica particles was conducted at 35°C by introducing amino groups on the silica particles with (3-aminopropyl)trimethoxysilane at pH 3 (NH2/SiO2) and then making Gd-EDTA act on the NH2/SiO2 p
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18

Kobayashi, Yoshio, Tetsuya Ayame, Kyosuke Shibuya, et al. "Stabilization of silica-coated silver iodide nanoparticles by ethanol-washing." Pigment & Resin Technology 45, no. 2 (2016): 99–105. http://dx.doi.org/10.1108/prt-10-2014-0083.

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Purpose – This paper aims to propose a simple method for stabilizing silica-coated silver iodide (AgI/SiO2) core-shell particles, of which a colloid solution functions as an X-ray contrast agent. Design/methodology/approach – A colloid solution of AgI nanoparticles was prepared by mixing silver perchlorate and potassium iodide in water. The AgI/SiO2 nanoparticles were fabricated by a sol-gel method using NaOH, H2O and tetraethylorthosilicate in ethanol in the presence of AgI nanoparticles surface-modified with 3-mercaptopropyltrimethoxysilane. Findings – The silica shells of AgI/SiO2 particles
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19

Cheng, Gang, Hong Chen, Kai Wang, et al. "Biphasic Calcium Phosphate/Chitosan/Polyacrylonitrile/Polylactic Acid-Glycolic Acid (PLGA) Nanocomposite Stent for Repair and Osteogenesis of Oral Alveolar Bone Defect." Science of Advanced Materials 13, no. 7 (2021): 1324–34. http://dx.doi.org/10.1166/sam.2021.4039.

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ABSTRACTThe ability of sol-gel micro-nano biphasic calcium stent to repair oral alveolar bone defects was investigated in this study, and its osteogenesis performance was also analyzed. Biphasic calcium phosphate (BCP) was synthesized by wet method, which was combined with chitosan (CS), polyacrylonitrile (PAN), and polylactic acid-glycolic acid (PLGA). Then, the BCP/CS/PAN/PLGA nanocomposite stent was prepared by vacuum freeze-drying technology. The micro-nano composite stent was combined with the bone morphogenetic protein-2 (BMP-2) gene, so as to obtain the sol-gel micro-nano biphasic calci
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20

Аймбетова, И. О., Э. О. Аймбетова, О. С. Байгенженов, Д. Берди, and Б. Умиров. "DEVELOPMENT OF A V2O5 THIN FILM DEPOSITION TECHNIQUE." Вестник ГГНТУ. Технические науки, no. 4(30) (December 22, 2022): 38–48. http://dx.doi.org/10.34708/gstou.2022.47.67.005.

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На основании технологического и экономического анализа самым оптимальным и технологически приемлемым методом получения термохромных композиций является легирование пленок фазопереходными материалами, позволяющих управлять основными параметрами энергосбережения и других термосвойств, с последующим нанесением на стеклянные и/или другие подложки. Исследован рост кристаллитов поликристаллических пленок, изготовленных путем одновременной вариации толщины напыленной пленки оксида ванадия и времени ее термообработки при различных температурах. Исследование структуры пленки осуществлено рентгеновским
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21

Zhang, Raymond, Timofey Averianov та Ekaterina Pomerantseva. "Assembly of Two-Dimensional δ-V2O5-Ti3C2Tx Heterostructure Electrodes for Li-Ion Batteries". ECS Meeting Abstracts MA2022-02, № 2 (2022): 150. http://dx.doi.org/10.1149/ma2022-022150mtgabs.

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The bilayered phase of vanadium oxide (δ-V2O5∙nH2O or BVO) synthesized via sol-gel synthesis method is an attractive candidate to be used as a cathode material for Li-ion batteries (LIBs) due to its expanded interlayer spacing, and high theoretical capacity. However, poor electrical conductivity and rapid capacity fade present challenges for achieving high-rate performance and good cycle life during cycling in LIBs. A viable solution to mitigating these drawbacks is to fabricate two-dimensional (2D) heterostructures comprised of vanadium oxide and an additional selected 2D material. Titanium c
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22

Kostryukov, Victor F., Irina Y. Mittova, Boris V. Sladkopevtsev, Anna S. Parshina та Dar’ya S. Balasheva. "Роль BiPO4, вводимого через газовую фазу, в процессе создания тонких пленок на поверхности InP". Kondensirovannye sredy i mezhfaznye granitsy = Condensed Matter and Interphases 21, № 2 (2019): 215–24. http://dx.doi.org/10.17308/kcmf.2019.21/759.

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Исследованием термооксидирования фосфида индия под воздействием фосфата висмута, вводимого через газовую фазу, установлено ускоряющее воздействие фосфата висмута на процесс формирования пленок. Величина ускорения составляет от 1.5 до 2 раз, и максимальный прирост пленки достигается в первые 10 мин оксидирования. Определяющим процессом является образование фосфата индия за счет вторичного взаимодействия оксидных форм компонентов подложки, лимитируемое диффузией оксидов в твердой фазе. Методами инфракрасной спектроскопии, локального рентгеноспектрального микроанализа и рентгенофазового анализа у
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23

Jiang, Hang, Hengxing Yu, Xin Guan, et al. "Harnessing the Power of Nature: Monodisperse Pickering Emulsion Droplets and Yolk‐Shell Microcapsules Utilizing Bee Pollen Particles." Advanced Functional Materials, February 15, 2024. http://dx.doi.org/10.1002/adfm.202316510.

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AbstractThe preparation of monodisperse Pickering emulsions currently can only be achieved using microfluidics or membrane emulsification, limiting its widespread applications in many fields. Here, by simply introducing naturally occurring pollen grains during mechanical emulsification, it becomes extremely feasible to fabricate uniform Pickering emulsions. The process of shearing and turbulence leads to a constant reduction in the size of the droplets of the dispersed phase. The robust exine of the pollen grains in the continuous phase enables them to retain their structural integrity during
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24

Nikoorazm, Mohsen, Maryam Khanmoradi, and Masoumeh Sayadian. "Nickel (II) and oxovanadium (IV) complexes supported on MCM-41 mesoporous and their application in catalytic selective sulfide and thiol oxidation." Combinatorial Chemistry & High Throughput Screening 23 (November 11, 2020). http://dx.doi.org/10.2174/1386207323999201111192649.

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Introduction:: MCM-41 was synthesized using the sol-gel method. Then two new transition metal complexes of Nickel (II) and Vanadium (IV), were synthesized by immobilization of adenine (6-aminopurine) into MCM-41 mesoporous. The compounds have been characterized by XRD, TGA, SEM, AAS and FT-IR spectral studies. Using these catalysts provided an efficient and enantioselective procedure for oxidation of sulfides to sulfoxides and oxidative coupling of thiols to their corresponding disulfides using hydrogen peroxide at room temperature. Materials and Methods:: To a solution of sulfide or thiol (1
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25

Dyadenchuk, Alena, Oleksenko Roman, and Yury Kuris. "Structural and electrical characteristics of the ZnO/porous-Si/Si heterostructure: from synthesis to analysis of photocell efficiency." International Journal of Mathematics and Physics 15, no. 2 (2024). https://doi.org/10.26577/ijmph.2024v15i2b4.

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The article deals with the step-by-step production of the ZnO/porous-Si/Si heterostructure: electrochemical etching of monocrystalline Si plates (100); deposition of ZnO films by the sol-gel method followed by centrifugation. A comprehensive study of the morphology of the obtained structure using a scanning electron microscope and an atomic force microscope confirms the high degree of structural order of the obtained film and the presence of the main chemical elements of the ZnO:Al film on its surface. It was established that the thickness of the ZnO:Al film is 395 nm, and the thickness of the
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26

Krenev, I. A., and M. N. Berlov. "ELABORATION OF A METHOD OF LYSOZYME PURIFICATION FROM HUMAN SALIVA." Problems of Biological, Medical and Pharmaceutical Chemistry, February 10, 2025, 40–47. https://doi.org/10.29296/25877313-2025-02-06.

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Introduction. Growing antibiotic resistance dictates the need for search for alternative antimicrobial drugs. Antimicrobial polypeptides, including lyso-zyme, are among prospective prototypes. Existing methods of human lysozyme purification are less elaborated in comparison with methods of lyso-zyme purification from other species sources. The aim of the study. Development of a simple and rapid method of lysozyme purification from human saliva, which can be performed in laborato-ries equipped for research of peptides and low-molecular proteins. Material and methods. Human saliva was treated wi
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