Relevant bibliographies by topics / Solid-state bioreactor

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Academic literature on the topic 'Solid-state bioreactor'

Author: Grafiati
Published: 4 June 2021
Last updated: 16 February 2022

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Journal articles on the topic "Solid-state bioreactor"

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Lim, B. R., H. Y. Hu, N. Goto, and K. Fujie. "PVA-coated activated carbon for aerobic biological treatment of concentrated refractory organic wastewater." Water Science and Technology 42, no. 3-4 (August 1, 2000): 205–10. http://dx.doi.org/10.2166/wst.2000.0381.

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The treatment characteristics of concentrated p-phenol sulfonic acid (PSA) wastewater in a submerged bioreactor and a solid phase bioreactor packed with ACP particles (polyvinyl alcohol particles coated with powered activated carbon) were compared experimentally. The changes in biomass and microbial community with the degradation of PSA at both bioreactors were also evaluated using microbial quinones as an index. Greater than 95% of influent PSA was mineralized at the solid phase bioreactor under the volumetric loading of PSA ranging from 0.3 to 1.8 kg-C·m-3·d-1 at the steady state, but less than 10% of the influent PSA was mineralized in the submerged bioreactor. The solid phase aerobic biological treatment process was more effective for the treatment of concentrated refractory chemicals such as PSA than the submerged bioreactor. The dominant quinone species in the solid phase bioreactor were ubiquinone-10 and menaquinone-8(H4), while those in the submerged bioreactor were ubiquinone-8 and menaquinone-8. This suggests hat different microbes had contributed to the degradation in the two bioreactors.
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Durand, A. "Bioreactor designs for solid state fermentation." Biochemical Engineering Journal 13, no. 2-3 (March 2003): 113–25. http://dx.doi.org/10.1016/s1369-703x(02)00124-9.

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da Cunha, Daniele Colembergue, Jeferson Avila Souza, Luiz Alberto Oliveira Rocha, and Jorge Alberto Vieira Costa. "Hexahedral modular bioreactor for solid state bioprocesses." World Journal of Microbiology and Biotechnology 25, no. 12 (July 24, 2009): 2173–78. http://dx.doi.org/10.1007/s11274-009-0122-3.

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Durand, A., D. de la Broise, and H. Blachère. "Laboratory scale bioreactor for solid state processes." Journal of Biotechnology 8, no. 1 (May 1988): 59–66. http://dx.doi.org/10.1016/0168-1656(88)90068-5.

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Ashok, Anup, Kruthi Doriya, Devulapally Ram Mohan Rao, and Devarai Santhosh Kumar. "Design of solid state bioreactor for industrial applications: An overview to conventional bioreactors." Biocatalysis and Agricultural Biotechnology 9 (January 2017): 11–18. http://dx.doi.org/10.1016/j.bcab.2016.10.014.

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Virtanen, Veera, Antti Nyyssölä, Antti Vuolanto, Matti Leisola, and Pekka Seiskari. "Bioreactor for solid-state cultivation of Phlebiopsis gigantea." Biotechnology Letters 30, no. 2 (September 22, 2007): 253–58. http://dx.doi.org/10.1007/s10529-007-9538-8.

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Chen, Hongzhang, and Qin He. "A novel structured bioreactor for solid-state fermentation." Bioprocess and Biosystems Engineering 36, no. 2 (July 8, 2012): 223–30. http://dx.doi.org/10.1007/s00449-012-0778-1.

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Sella, Sandra Regina Barroso Ruiz, Belquis Palácio Guizelini, Luciana Porto de Souza Vandenberghe, Adriane Bianchi Pedroni Medeiros, and Carlos Ricardo Soccol. "Lab-Scale production of Bacillus atrophaeus' spores by solid state fermentation in fifferent types of bioreactors." Brazilian Archives of Biology and Technology 52, spe (November 2009): 159–70. http://dx.doi.org/10.1590/s1516-89132009000700021.

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Studies were conducted to evaluate Bacillus atrophaeus spores' production by solid-state fermentation (SSF) using sugarcane bagasse as support and soybean molasses as substrate at lab-scale in column bioreactors (forced aeration), plastic bags and Erlenmeyer flasks (aeration by diffusion). Different moisture contents (84%, 86% and 88%; 89%, 91% and 93%) and aeration rates (30mL/min, 45mL/min, 60mL/min and 90mL/min) were studied. The best condition for spore production (3.3x10(10) CFU.g-¹dry matter) in column bioreactor was 80% of initial humidity and no aeration. In Erlenmeyer flasks and plastic bags the best sporulation production reached 1.7 up to 4.7x10(10) CFU.g-1dry matter with 88-93% of initial moisture. The aeration rate had no significant effect on the spore yield. The initial moisture had a significant effect depending on the bioreactor type. Sporulation kinetic's assay was carried out and it showed the possibility to reduce the time of spore formation in two days.
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Lee, Ho Yong, Won-Rok Kim, and Bong Hee Min. "Automation of Solid-state Bioreactor for Oyster Mushroom Composting." Mycobiology 30, no. 4 (2002): 228. http://dx.doi.org/10.4489/myco.2002.30.4.228.

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Kalogeris, E., G. Fountoukides, D. Kekos, and B. J. Macris. "Design of a solid-state bioreactor for thermophilic microorganisms." Bioresource Technology 67, no. 3 (March 1999): 313–15. http://dx.doi.org/10.1016/s0960-8524(98)00124-2.

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Dissertations / Theses on the topic "Solid-state bioreactor"

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Myers, Michael John. "Laboratory Scale Solid State Landfill Bioreactor Design." The Ohio State University, 1999. http://rave.ohiolink.edu/etdc/view?acc_num=osu1393077896.

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Silva, Ellen Mae. "A gas-solid spouted bed bioreactor for solid state fermentation /." The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487945320759412.

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Abdul, Manan Musaalbakri. "Design aspects of solid state fermentation." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/design-aspects-of-solid-state-fermentation(d64ea506-85ee-424f-9bca-531488e3e3c7).html.

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Solid state fermentation (SSF) refers to the microbial fermentation, which takes place in the absence or near absence of free water, thus being close to the natural environment to which the selected microorganisms, especially fungi, are naturally adapted. The current status of SSF research globally was discussed in terms of articles publication. This was followed by discussion of the advantages of SSF and the reason for interest in SSF as a notable bioprocessing technology to be investigated and compared to submerged fermentation (SmF) for the production of various added-value products. SSF also proved to be a potential technology to treat solid waste produced from food and agricultural industry and to provide environmental benefits with solid waste treatment. A summary was made of the attempts at using modern SSF technology for future biorefineries for the production of chemicals. Many works were carried out in the Satake Centre for Grain Process Engineering (SCGPE), University of Manchester, to prove the strategy of using SSF for the production of hydrolysate rich in nutrients for sequel microbial fermentation with or without adding any commercial nutrients. The research findings presented in this thesis are based on a series of SSF experiments carried out on systems varying in complexity from simple petri dishes to our own design of bioreactor systems. They were conducted to assess a solution for biomass estimation, enzymes production, and successful mass and heat transfer. A proper technique for inoculum transfer prior to the start of the fermentation process was developed. In SSF, estimation of biomass presents difficulties as generally the fungal mycelium penetrates deep and remains attached with the solid substrate particles. Although many promising methods are available, the evaluation of microbial growth in SSF may sometimes become laborious, impractical and inaccurate. Essentially, this remains another critical issue for monitoring growth. In these studies, measurement of colour changes during SSF are presented as one of the potential techniques that can be used to describe growth, complementary to monitoring metabolic activity measurement, such as CER, OUR and heat evolution, which is directly related to growth. For the growth of Aspergillus awamori and Aspergillus oryzae on wheat bran, soybean hulls and rapeseed meal, it was confirmed that colour production was directly proportional to fungal growth. This colourimetric technique was also proved to be a feasible approach for fungal biomass estimation in SmF. This new approach is an important complementation to the existing techniques especially for basic studies. The key finding is that the colourimetric technique demonstrated and provided information of higher quality than that obtained by visual observation or spores counting. The effect of aeration arrangements on moisture content, oxygen (O2), mass and heat transfer during SSF was investigated. A. awamori and A. oryzae were cultivated on wheat bran in newly designed four tray solid state bioreactor (SSB) systems. The new tray SSB systems were: (1) single circular tray SSB, (2) multi-stacked circular tray SSB, (3) Single rectangular tray SSB and (4) multi-square tray SSB. The purpose was to study the effect, on heat and water transfer, of operating variables, fermentation on the perforated base tray and internal moist air circulation under natural and forced aeration. Temperature, O2 and carbon dioxide were measured continuously on-line. Enzyme activity, moisture content and biomass were also measured. The results suggest that the air arrangements examined have a remarkable effect on the quantity of biomass produced using measurement of spores and enzymes production. The strategy presented in these studies allowed quantitative evaluation of the effect of forced internal moist air circulation on the removal of metabolic heat. With the proposed strategy, it was possible to maintain the bed temperatures at the optimum level for growth. However, the effect on moisture content was very different for the two fungi. It was found that the ability of A. oryzae to retain moisture was much higher than that of A. awamori. This is possibly due to the higher levels of chitin in A. oryzae. Greater spores and enzymes (glucoamylase, xylanase and cellulase) production was observed for A. awamori in multi-stacked circular tray and multi-square tray SSB systems compared to the conventional petri dishes and the other two systems. A. oryzae was excellent in producing protease in the same bioreactors. A direct technique of establishing a correlation between fungal growth and CER, OUR, heat evolved was proven successful in this work. The information obtained from CER and OUR led to the estimation of respiratory quotient (RQ). RQ describes the state of the fungal population in the tray SSB and gives an indication of fungal metabolic behaviour. RQ values < 1 were obtained from 38 experiments using four tray SSB systems for the two fungi. A kinetic model based on CO2 evolution instead of biomass concentration was examined in order to simplify the required experiments for kinetic model development. A Gompertz model was used to fit the integrated CO2 data and predict the quantity of CO2 evolution in all experiments. A correlation was found between the heat evolution and CER. The performances of tray SSB systems can be improved by constructing them as multi-trays. The multi-tray systems improved the mass transfer considerably compared with single tray systems. In addition, the multi-tray systems allowed precise measurement of the gradients of CO2, enzymes, spores and fungal biomass. In addition, the air arrangements using moistened air were successful in maintaining moisture content, adequate O2 supply and control of temperature, and hence, increased the productivity of both fungi. Overall A. awamori and A. oryzae have their own ability and performance to degrade and utilise the complex compositions contained in the solid substrate and fermentation conditions may lead to possible comparisons. In addition, multi-stacked circular tray and multi-square tray SSB systems demonstrated an excellent system for further investigations of mass transfer and possibly for large scale operation, though considerable optimisation work remains to be done, for example the height/diameter ratio and total number of trays should be optimised.
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Brijwani, Khushal. "Solid state fermentation of soybean hulls for cellulolytic enzymes production: physicochemical characteristics, and bioreactor design and modeling." Diss., Kansas State University, 2011. http://hdl.handle.net/2097/8401.

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Doctor of Philosophy
Department of Grain Science and Industry
Praveen V. Vadlani
The purpose of this study was to investigate micro- and macro-scale aspects of solid state fermentation (SSF) for production of cellulolytic enzymes using fungal cultures. Included in the objectives were investigation of effect of physicochemical characteristics of substrate on enzymes production at micro-scale, and design, fabrication and analysis of solid-state bioreactor at macro-scale. In the initial studies response surface optimization of SSF of soybeans hulls using mixed culture of Trichoderma reesei and Aspergillus oryzae was carried out to standardize the process. Optimum temperature, moisture and pH of 30ºC, 70% and 5 were determined following optimization. Using optimized parameters laboratory scale-up in static tray fermenter was performed that resulted in production of complete and balanced cellulolytic enzyme system. The balanced enzyme system had required 1:1 ratio of filter paper and beta-glucosidase units. This complete and balanced enzyme system was shown to be effective in the hydrolysis of wheat straw to sugars. Mild pretreatments– steam, acid and alkali were performed to vary physicochemical characteristics of soybean hulls – bed porosity, crystallinity and volumetric specific surface. Mild nature of pretreatments minimized the compositional changes of substrate. It was explicitly shown that more porous and crystalline steam pretreated soybean hulls significantly improved cellulolytic enzymes production in T. reesei culture, with no effect on xylanase. In A. oryzae and mixed culture this improvement, though, was not seen. Further studies using standard crystalline substrates and substrates with varying bed porosity confirmed that effect of physicochemical characteristics was selective with respect to fungal species and cellulolytic activity. A novel deep bed bioreactor was designed and fabricated to address scale-up issues. Bioreactor’s unique design of outer wire mesh frame with internal air distribution and a near saturation environment within cabinet resulted in enhanced heat transfer with minimum moisture loss. Enzyme production was faster and leveled within 48 h of operation compared to 96 h required in static tray. A two phase heat and mass transfer model was written that accurately predicted the experimental temperature profile. Simulations also showed that bioreactor operation was more sensitive to changes in cabinet temperature and mass flow rate of distributor air than air temperature.
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Amodu, Olusola S. "Biodegradation of polycyclic aromatic hydrocarbon contaminants in a mixed culture bioreactor." Thesis, Cape Peninsula University of Technology, 2015. http://hdl.handle.net/20.500.11838/934.

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Thesis submitted in fulfilment of the requirements for the degree of Doctor Technologiae: Chemical Engineering, Faculty of Engineering at the Cape Peninsula University of Technology - Cape Town, South Africa
Polycyclic aromatic hydrocarbons (PAHs) are one of the most common and recalcitrant environmental contaminants – known for their potential toxicity, mutagenicity, and carcinogenicity to humans. Biosurfactant application can enhance the biodegradation of PAHs. The main object of this work was to explore the novelty of biosurfactant produced by the isolated strains of Bacillus sp and Pseudomonas aeruginosa grown exclusively on Beta vulgaris, and the modification of the zeolites nanoparticles by the biosurfactant, for enhanced biodegradation of PAHs in soil. Novel biosurfactant-producing strains were isolated from hydrocarbon-contaminated environments, while several agrowaste were screened as primary carbon sources for the expression of biosurfactants, which were quantified using various standardized methods......
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Umstead, Russell Blake. "Development of Fungal Bioreactors for Water Related Treatment and Disinfection Applications." Thesis, Virginia Tech, 2016. http://hdl.handle.net/10919/72291.

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Wastewater, recycled irrigation water, and agricultural runoff can contain high levels of pathogenic bacteria, which pose a threat to human and ecosystem health. The use of a bioreactor containing mycelial mats of filamentous fungi is a novel treatment technology that incorporates physical, biological, and biochemical processes to remove bacterial pathogens from influent water. Although a relatively new concept, fungal bioreactors have demonstrated the ability to dramatically reduce fecal coliform bacteria in water, but no studies have attempted to explicitly identify the bacterial pathogen removal mechanisms exhibited by the fungi. This study evaluated several different species of fungi for use in fungal bioreactor systems and aimed to identify the modes of action responsible for the removal of bacterial pathogens. The species evaluated were Daedaleopsis confragosa, Pleurotus eryngii, and Piptoporus betulinus. Experimental results showed that all species of fungi assessed were capable of removing E. coli in a synthetic water solution. Significant concentrations of hydrogen peroxide, an antiseptic, were produced by all species of fungi evaluated. The fungal bioreactors containing P. eryngii produced the highest concentrations of hydrogen peroxide, generating a maximum concentration of 30.5 mg/l or 0.896 mM. This maximum value exceeds reported minimum concentrations required to demonstrate bacteriostatic and bactericidal effects when continually applied, providing evidence that a major bacterial removal mode of action is the production of antimicrobial compounds. In addition to its promising application to improve water quality, fungal bioreactors are a low cost and passive treatment technology. The development a hyper-functional system could be a have a substantial impact on the use of recycled irrigation water and on the water/wastewater treatment industry, for both municipal and agricultural wastewater.
Master of Science
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Ricky, Ricky. "Uppskalning av en svampkaka : process från avfallsbröd med en ätlig svamp." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-23882.

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Stale bread contributes to the biggest volume of food waste in Sweden. Current method on recovering bread waste is by producing biogas or bioethanol. Despite advantages in the energy sector, the bread which still has relatively high quality could be recovered into new products with higher value, such as food for human consumption. Development of a product, termed ‘fungal cake’ by solid state fermentation on bread waste using Neurospora intermedia in small scale petri dishes have previously been successfully conducted. This study aims to scale up the production of fungal cake into bench scale production. Two systems using different bioreactors were used in this study. The first system operated in batch mode using a tray bioreactor, in which the effect of particle size, mixing, and bread loading were evaluated. The fermentation was conducted during 5 days. Bread crumb with a larger particle size of 2 mm resulted in similar outcomes as bread crumb with a smaller particle size of 0.5 mm in terms of CO2 evolution rate, cumulative CO2 production, starch, and protein content of the final product. However, larger particle size resulted in a more homogeneous growth of the fungus throughout the product, which is preferred. The presence of daily mixing had no significant effect compared to static condition for all measured variables. Thus, mixing could be introduced to promote product homogeneity. Likewise, bread loading had no significant effect on the measured variables, which implies that a higher productivity can be achieved using a higher bread loading. The second system operated in continuous mode using a newly developed continuous tubular bioreactor with product recycle. Two experiments, in which the residence time (48h and 24h) and recycle ratio (10/65 and 20/55) were conducted. Both experiments yielded product with stable starch and protein content, indicated by a stable CO2 evolution rate over time. The performance using continuous tubular bioreactor was compared to batch fermentation in tray bioreactor using the same ratio of inoculum and both system yielded product with the same starch and protein content. Successful operation in continuous bioreactor certainly improved the productivity of fungal cake production.
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Cunha, Daniele Colembergue da. "Modelagem, simulação e otimização de biorreatores de leito fixo para fermentação/bioprocesso em estado sólido." reponame:Repositório Institucional da FURG, 2009. http://repositorio.furg.br/handle/1/6096.

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Ao contrário dos bioprocessos submersos, que são amplamente utilizados e estudados, bioprocessos em estado sólido (BES) ainda são carentes de estudos de modelagem e simulação, o que aponta para o grande potencial de otimização. A dificuldade no aperfeiçoamento de BES está associada a problemas com a dissipação do calor gerado pelas atividades metabólicas do microrganismo durante o crescimento. Esta dificuldade na transferência de calor dentro do biorreator pode levar a zonas de altas temperaturas, que afetam adversamente a produtividade. A modelagem matemática é uma ferramenta essencial para otimizar bioprocessos. Através de modelos matemáticos é possível otimizar as variáveis operacionais para controle do bioprocesso e também analisar o design do biorreator. A otimização geométrica, de acordo com a Teoria Constructal, visa melhorar o desempenho do biorreator através, por exemplo, de minimizar a temperatura no interior do leito a níveis ótimos para o cultivo. O presente trabalho apresenta projetos de biorreatores para BES, todos com geometria otimizada, obtidos a partir de experimentação numérica, através de um software de computational fluid dynamics (CFD). O modelo matemático utilizado era preditivo e significativo ao nível de confiança de 95%. A otimização geométrica foi apresentada em função das condições operacionais do cultivo. Para o biorreator de coluna e leito fixo com paredes isoladas, foram apresentadas as geometrias ótimas em função da velocidade, da vazão e da temperatura do ar de admissão. Para uma temperatura do ar de admissão de 29,5 ºC, as configurações ótimas ((D/L)opt) variaram entre 1,0 e 2,4 para uma faixa de velocidade de admissão do ar entre 0,003 e 0,006 m s-1 . Relacionando com vazão, as razões mostraram-se ótimas entre 2,2 ≤ (D/L) ≤ 2,6 quando operando sob 3,3 a 3,5 10-5 m3 s-1 . Outro biorreator estudado foi o biorreator modular, composto de módulos elementares com geometria otimizada, sendo adaptável a diferentes escalas de produção e de fácil montagem. As configurações ótimas dos módulos de geometria retangular e seção quadrada foram apresentadas para diferentes volumes de módulos, em função da temperatura e da velocidade do ar de admissão. Foi observado que o volume máximo do módulo sem resfriamento externo é 5 L, para uma velocidade do ar de admissão acima de 0,0045 m s-1 e temperatura inferior ou igual 29,0 ºC O último biorreator proposto foi o biorreator hollow, semelhante a um biorreator de coluna e leito fixo, porém com um duto oco inserido nele. O duto interno tem inúmeros furos perpendiculares às suas paredes, mas sua saída é isolada, permitindo que o ar penetre no meio poroso. A geometria otimizada do biorreator hollow foi apresentada em função da fração de volume do duto interno, da razão entre os diâmetros de entrada e saída do duto interno, da vazão e da temperatura do ar de admissão. Em comparação com o biorreator de coluna convencional de mesmas dimensões e sob mesmas condições operacionais, o biorreator hollow apresentou temperatura máxima mais baixa, demonstrando que o projeto é eficiente para resfriar o meio poroso. Concluiu-se, enfim, no presente trabalho, que a geometria é um parâmetro importante e a sua otimização pode beneficiar o desempenho do biorreator.
Unlike the submerged bioprocesses, that was wildly used and studied, solid state bioprocess (SSB) are still poorly studied with respect to modeling and simulation, what indicates a big potential for optimization. The difficulty in the BES improvement is associated to problems with the dissipation of heat generated by metabolic activities of microorganisms during growth. This difficulty in transferring heat from the bioreactor could lead to areas with high temperature, which usually affect the productivity adversely. The mathematical modeling is an essential tool for optimizing bioprocesses. Using mathematical models it is possible to optimize operational variables to control the bioprocess and also explore the design of the bioreactor. The geometric optimization, according Constructal Theory, aims to improve the performance of the bioreactor through, for example, minimizing the temperature inside the bed to optimum levels for the bioprocess. The present work presents designs of bioreactors to SSB, all with optimized geometry, obtained from numerical experiments, by computational fluid dynamics (CFD) software. The mathematical model used has been predictive and significant at .95 level of confidence. The geometric optimization was presented as function of operational conditions of the cultivation. For the column fixed bed bioreactor with isolated wall, the optimal configurations are shown as function of flow, velocity and temperature of inlet air. For a inlet air temperature of 29.5 ºC, the optimal configurations ((D/L)opt) varied between 1.0 e 2.4 to a range of inlet velocity between 0.003 e 0.006 m s-1 . Relating with the volumetric flow, the optimal ratios presented between 2.2 ≤ (D/L) ≤ 2.6 when operating under 3.3 a 3.5 10-5 m3 s-1 . Other studied bioreactor was the modular bioreactor, consisting of elementary modules with optimized geometry, being adaptable to different scales of production and easy assembly. The optimal configurations of the modules with rectangular geometry and square section were shown depending on the volume of modules and the temperature and velocity of inlet air. It was observed that the maximum volume of the module without external cooling was 5 L, for a inlet velocity upper 0.0045 m s-1 and temperature smaller or equal to 29.0 ºC. The last proposed bioreactor was the hollow bioreactor, similar to a column fixed bed bioreactor, but with an empty duct inserted on it. The internal duct has innumerable holes perpendicular to its wall (the inlet port), but its end is insulated, allowing the air penetrates into the porous medium. The optimized geometry of hollow bioreactor was presented in function of the volume fraction of internal duct, the ratio between the diameters of inlet and outlet of the internal duct, the flow rate and temperature of the inlet air. Comparing with the conventional column bioreactor with the same configuration and same operational conditions, the hollow bioreactor showed a lower maximum temperature. This demonstrates that the project is efficient at cooling the porous medium. Finally, it was concluded that the geometry is an important parameter and its optimization can benefit the performance of the bioreactor.
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Carboué, Quentin. "Mise au point d'un bioréacteur de fermentation en milieu solide fonctionnant en continu pour la production de métabolites secondaires antioxydants par Aspergillus niger G131." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0136.

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Aspergillus niger souche G131 est un champignon qui produit en quantité des métabolites secondaires appartenant à la famille des naphtho-gamma-pyrones (NγPs). Ces NγPs sont des pigments qui présentent des intérêts industriels de par leurs importants potentiels antiradicalaires. L’objectif de ce doctorat est la production à l’échelle pilote et en continu de NγPs à travers la culture du champignon sur milieu solide. Le choix de la fermentation en milieu solide (FMS) comme processus de culture repose sur des aspects d’ordre qualitatif et quantitatif de production, ainsi que sur des raisons économiques et éthiques, relatives à la protection de l’environnement avec notamment la possibilité de valoriser des coproduits agricoles comme milieu de culture pour le champignon. Dans un premier temps, ce travail s’intéresse à la caractérisation de la composition et des potentialités associées aux molécules produites par la souche. Ces potentialités incluent les activités anti-radicalaires et les mesures de cytotoxicité. La thèse porte également sur la caractérisation de la physiologie de croissance de la souche en FMS et sur l’optimisation des conditions de culture par la méthodologie des plans d’expériences pour l’augmentation de la production de NγPs. Une stratégie originale d’optimisation adaptée aux contraintes posées par la FMS est d’ailleurs proposée. Finalement, un transfert d’échelle de production est réalisé au moyen d’un bioréacteur prototype innovant permettant la production à l’échelle pilote de milieu fermenté en continu. Dans son dernier chapitre, ce travail s’intéresse donc à la mise au point des paramètres opératifs qui entourent la production continue de NγPs par FMS
Aspergillus niger strain G131 is a non-ochratoxigenic filamentous fungus producing high quantities of secondary metabolites known as naphtha-gamma-pyrones (NγPs). NγPs are pigments of industrial interest in reason of their high antioxidant properties. The aim of this dissertation is the continuous, pilote-scaled production of these NγPs through the cultivation of the fungus on solid medium. The choice of solid state fermentation (SSF) as cultivation method is not only driven by quantitative and qualitative considerations, but also by economical and ethical concerns related to environmental protection. SSF allows, in fact, a direct valorization of agricultural byproducts as the solid medium for the fungal growth. First, this work deals with the characterization of the composition and potentialities associated with the molecules produced by the strain, which include antioxidant and cytotoxic activities. Second, the dissertation focuses on the characterization of the fungal growth’s physiology on solid medium and on the optimization of the culture conditions using experimental methodology in order to increase NγPs production. For this purpose, an original optimization strategy is proposed to overcome specific constraints connected to SSF. Finally, a scale transfer of the production is advanced by means of an innovative prototype bioreactor continuously producing fermented material. The final chapter of this work addresses the development of parameters regarding the continuous NγPs production using SSF
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Pirota, Rosangela Donizete Perpetua Buzon. "Simplificação do processo de conversão de biomassa a etanol usando enzimas do meio fermentado integral de fungos filamentosos cultivados por fermentação em estado sólido." Universidade Federal de São Carlos, 2013. https://repositorio.ufscar.br/handle/ufscar/268.

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The main challenge on the conversion of lignocellulosic biomass into liquid fuels is the economic viability of this process. Thus, the commercialization of lignocellulosic ethanol is hindered mainly by the high costs of the enzyme preparations currently available cellulases - enzymes used in the saccharification step. Some strategies that can be adopted to reduce the enzymes costs include selecting microorganisms, use of cheaper raw materials and more efficient fermentation strategies such as the solid state fermentation (SSF) and efficient techniques for saccharification and fermentation. The aim this work was evaluate the use of the whole fermentation medium containing lignocellulosic biomass, fungal mycelium and enzymes in the hydrolysis of sugarcane bagasse pretreated by steam explosion for cellulosic ethanol production. In this context, a selection of filamentous fungi highly producing cellulases and hemicellulases, optimization operating conditions, such as humidity and temperature, were carried out for in house enzyme production using an instrumented bioreactor. Then, the efficiency of the whole fermentation medium and enzyme extract in enzymatic hydrolysis of lignocellulosic biomass for cellulosic ethanol production was evaluated. Among the 40 fungal strains evaluated, two strains of A. oryzae (P6B2 and P27C3A) stood out. In addition, one strain of A. niger 3T5B8 and another of T. reesei RUT C30 were also evaluated in this study. The influence of the substrate initial moisture content and temperature on efficiency of cellulase and xylanase production by strains of A. oryzae, A. niger and T. reesei grown in SSF under conditions of forced aeration and static were evaluated. The initial moisture content of the substrate did not affect the production of cellulases and xylanases by strain of A. oryzae P27C3A, however higher moisture was better for enzyme production by strains of A. oryzae P6B2 and A. niger and lower moisture were better for the production of cellulases and xylanases by T. reesei in both cultive systems. Temperature 28°C was best for xylanase production by all the fungal strains, while higher temperatures was better cellulases production in both culture systems. The use of whole fermented medium of A. niger or T. reesei obtained in the bioreactor were better in the hydrolysis sugarcane bagasse pretreated by steam explosion (BPSE) than the enzymatic extract with a final conversion of 41.3 and 24.9% of theoretical, respectively. The combination of whole fermentation medium of strains of A. oryzae (P6B2 or P27C3A) obtained in flasks and ½ commercial enzyme hydrolysis also were efficient on BPSE hydrolysis (26.1 and 42.4% of theoretical, respectively). Nevertheless, the combination of whole fermented medium of A. oryzae P6B2 and enzymatic extract of A. niger obtained in flasks promoted a conversion of 65% and an ethanol yield of 84% of the theoretical value. As overall conclusion it was found that the use of whole fermented medium produced by fungi cultivated under solid state fermentation (SSF) in the BPSE hydrolysis resulted in similar or higher yields compared to the hydrolysis using the enzyme extract, giving clear indication that the extraction/filtration step of the enzyme can be eliminated. The use of the enzyme complex of A. oryzae P6B2 in combination with the enzymes of A. niger resulted in a BPSE hydrolysis more efficient when compared with other combinations, showing the importance of selecting microorganisms for high enzymes production. Moreover, the use of a single reactor system for performing enzyme production steps by SSF, saccharification and alcoholic fermentation may be performed, avoiding the need for steps separation.
A discussão dominante sobre a transformação da biomassa lignocelulósica a combustível líquido é a sua viabilidade econômica. Assim, a comercialização do etanol a partir de biomassa lignocelulósica é dificultada principalmente pelos custos proibitivos das preparações de celulases enzimas usadas na sacarificação. Algumas estratégias que podem ser adotadas para a redução do custo das enzimas utilizadas na degradação da biomassa incluem a seleção de micro-organismos altamente produtores de celulases e hemicelulases, utilização de matéria-prima mais barata e estratégias de fermentação a um custo efetivo - como a fermentação em estado sólido (FES) e técnicas mais eficientes de sacarificação e fermentação alcoólica. O objetivo deste trabalho foi avaliar a utilização do meio fermentado integral (MFI), contendo biomassa lignocelulósica, micélio fúngico e enzimas na hidrólise do bagaço de cana pré-tratado por explosão a vapor para produção de etanol celulósico. Neste contexto, realizou-se a seleção de fungos filamentosos isolados do solo de madeira em decomposição da Região Amazônica produtores de celulases e hemicelulases, otimizou-se as condições operacionais, como umidade e temperatura para a produção de enzimas in house utilizando biorreator de coluna instrumentado e por fim, avaliou-se a eficiência do MFI e (EE) na hidrólise enzimática da biomassa lignocelulósica para produção de etanol celulósico. Entre os 40 fungos caracterizados quanto à produção de enzimas envolvidas na degradação da lignocelulose, duas linhagens de A. oryzae (P6B2 e P27C3A) se destacaram em relação às demais. Além das linhagens de A. oryzae outras duas linhagens de fungos, uma de A. niger 3T5B8 e outra de T. reesei RUT C30 foram avaliadas neste trabalho, a fim de verificar a eficiência das linhagens isoladas do solo da Floresta Amazônica. A umidade inicial do substrato não influenciou na produção de celulases e xilanases pela linhagem de A. oryzae P27C3A, no entanto umidades elevadas foram melhores para a produção de enzimas pelas linhagens de A. oryzae P6B2 e A. niger e umidades baixas foram melhores para a produção de celulases e xilanases por T. reesei em ambos os sistemas de cultivo, forçado e estático. Com relação à temperatura de fermentação, 28ºC foi melhor para a produção de xilanases por todas as linhagens fúngicas e temperaturas mais elevadas favoreceram a produção de celulases pelos fungos. A utilização do MFI de A. niger ou T. reesei obtido em biorreator de coluna instrumentado foram melhores na hidrólise do bagaço de cana pré-tratado por explosão a vapor (BEX) do que o EE, com uma conversão final de 41,3 e 24,9% do valor teórico, respectivamente. A combinação de MFI das linhagens de A. oryzae (P6B2 ou P27C3A) obtida em Erlenmeyer e ½ de enzima comercial também favoreceram a hidrólise do BEX (26,1 e 42,4% do valor teórico, respectivamente). No entanto, a combinação de MFI de A. oryzae P6B2 e EE de A. niger obtido em Erlenmeyer promoveram uma conversão final de 65% e um rendimento de etanol de 84% do valor teórico. Vale salientar que foi utilizado na fermentação alcoólica o meio hidrolisado na íntegra, contendo açúcares, enzimas, biomassa lignocelulósica e micélio fúngico. Como conclusões gerais, constatou-se que a utilização de MFI produzido pelos fungos por FES na hidrólise do BEX resultou em rendimentos semelhantes ou mais elevados quando comparado com a hidrólise do BEX utilizando EE, dando a clara indicação de que o passo de extração/filtração das enzimas pode ser eliminado; a utilização do complexo enzimático de A. oryzae P6B2 em combinação com o complexo enzimático de A. niger resultou em uma hidrólise mais eficiente do BEX quando comparado com outras combinações, mostrando a importância da seleção de micro-organismos produtores de enzimas envolvidas na degradação da lignocelulose, para que a produção de etanol celulósico possa se tornar economicamente viável; e por fim, a utilização de um único sistema de reator para a realização das etapas de produção de enzimas por FES, sacarificação e fermentação alcoólica pode ser realizada, evitando-se a necessidade de etapas de filtração.
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Books on the topic "Solid-state bioreactor"

1

Mitchell, David A., Marin Berovič, and Nadia Krieger, eds. Solid-State Fermentation Bioreactors. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/3-540-31286-2.

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Mitchell, David A., Nadia Krieger, and Marin Berovic. Solid-State Fermentation Bioreactors: Fundamentals of Design and Operation. Springer, 2010.

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(Editor), David A. Mitchell, Nadia Krieger (Editor), and Marin Berovic (Editor), eds. Solid-State Fermentation Bioreactors: Fundamentals of Design and Operation. Springer, 2006.

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1962-, Mitchell David A., Krieger Nadia, and Berovic M, eds. Solid-state fermentation bioreactors: Fundamentals of design and operation. Berlin: Springer, 2006.

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Book chapters on the topic "Solid-state bioreactor"

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Mitchell, David A., Oscar F. von Meien, Luiz F. L. Luz, and Marin Berovič. "Substrate, Air, and Thermodynamic Parameters for SSF Bioreactor Models." In Solid-State Fermentation Bioreactors, 265–78. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/3-540-31286-2_19.

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Mitchell, David A., Oscar F. von Meien, Luiz F. L. Luz, and Nadia Krieger. "A Model of an Intermittently-Mixed Forcefully-Aerated Bioreactor." In Solid-State Fermentation Bioreactors, 349–62. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/3-540-31286-2_25.

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Mitchell, David Alexander, Luana Oliveira Pitol, Alessandra Biz, Anelize Terezinha Jung Finkler, Luiz Fernando de Lima Luz, and Nadia Krieger. "Design and Operation of a Pilot-Scale Packed-Bed Bioreactor for the Production of Enzymes by Solid-State Fermentation." In Solid State Fermentation, 27–50. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/10_2019_90.

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Byndoor, M. G., N. G. Karanth, and G. V. Rao. "Efficient and versatile design of a tray type solid state fermentation bioreactor." In Advances in Solid State Fermentation, 113–19. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-0661-2_9.

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Fernandez, M., J. Ananias, I. Solar, R. Perez, L. Chang, and E. Agosin. "Advances in the development of a control system for a solid substrate pilot bioreactor." In Advances in Solid State Fermentation, 155–68. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-0661-2_13.

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Mitchell, David Alexander, and Nadia Krieger. "Solid-State Cultivation Bioreactors." In Essentials in Fermentation Technology, 105–33. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-16230-6_4.

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Mitchell, David A., Penjit Srinophakun, Nadia Krieger, and Oscar F. von Meien. "Group II Bioreactors: Forcefully-Aerated Bioreactors Without Mixing." In Solid-State Fermentation Bioreactors, 77–94. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/3-540-31286-2_7.

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Mitchell, David A., Oscar F. von Meien, Luiz F. L. Luz, Nadia Krieger, J. Ricardo Pérez-Correa, and Eduardo Agosin. "Group IVb: Intermittently-Mixed Forcefully-Aerated Bioreactors." In Solid-State Fermentation Bioreactors, 129–40. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/3-540-31286-2_10.

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Mitchell, David A., Luiz F. L. Luz, Marin Berovič, and Nadia Krieger. "Approaches to Modeling SSF Bioreactors." In Solid-State Fermentation Bioreactors, 159–78. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/3-540-31286-2_12.

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Mitchell, David A., Luiz F. L. Luz, Marin Berovič, and Nadia Krieger. "Appropriate Levels of Complexity for Modeling SSF Bioreactors." In Solid-State Fermentation Bioreactors, 179–90. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/3-540-31286-2_13.

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Conference papers on the topic "Solid-state bioreactor"

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SILVEIRA, C. L. da, M. A. MAZUTTI, and N. P. G. SALAU. "SOLID-STATE FERMENTATION MODEL FOR A PACKED-BED BIOREACTOR." In XX Congresso Brasileiro de Engenharia Química. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeq2014-1963-16649-161342.

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Xu, Z., Y. Wang, Y. Chen, M. H. Spalding, and L. Dong. "MICROFLUIDIC MICROALGAL BIOREACTOR FOR HIGH-THROUGHPUT SCREENING OF CO2 CONCENTRATION CONDITIONS ON MICROALGAE GROWTH." In 2016 Solid-State, Actuators, and Microsystems Workshop. San Diego: Transducer Research Foundation, 2016. http://dx.doi.org/10.31438/trf.hh2016.14.

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Wang, Erqiang, Bing Han, and Shizhong Li. "Numerical simulation of transient radial temperature distribution in rotating drum bioreactor for solid state fermentation." In 2013 International Conference on Materials for Renewable Energy and Environment (ICMREE). IEEE, 2013. http://dx.doi.org/10.1109/icmree.2013.6893668.

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Gong, Song-En, Rong-Jhe Chen, Chung-Kuang Chin, Wen-Chen Chu, Chen-Ta Ho, Hwan-You Chang, and Cheng-Hsien Liu. "On-chip lobule-mimetic construction of heterogeneous cells and CO-cuture via a logarithmical-concentration varying bioreactor." In TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference. IEEE, 2009. http://dx.doi.org/10.1109/sensor.2009.5285618.

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Jeong, Yun-Jin, Bong-Kee Lee, Eung-Sam Kim, and Dong-Weon Lee. "A Novel Stage-Top-Bioreactor Integrated with Nano-Textured Polydimethylsiloxane (PDMS) Diaphragm." In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII). IEEE, 2019. http://dx.doi.org/10.1109/transducers.2019.8808454.

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Kawai, Hideki, Tomoki Honma, and Hiroshige Kikura. "Spectrum Analysis of the Taylor Vortex Flow With a Short Annulus Using the Ultrasonic Velocity Profiler." In ASME-JSME-KSME 2011 Joint Fluids Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajk2011-11026.

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Mild mixing effect is observed in the Taylor vortex flow (TVF) system in which the animal and plant cell walls are not disrupted as much as other bioreactor systems. These facts would be more important in developing a new bioreactor system, which plays an active role in the medical and bioindustrial fields. Although the mixing methods with an impeller are often used for an agitation in synthetic polymerization, the problem is that there exists a high shearing flow region just after the moving impeller. This region for instance, would cause the destruction of the alga cell when a microorganism in suspension is used as a bioreactor. The TVF is expected to reduce the local increase of these shearing forces because the device doesn’t have any impeller, and lead the bioreactor system to more uniform and stable states in the flow fields for cultivation or oxygen production. TVF in a single-phase flow with an infinite annulus is well known since it has a spectrum transition to turbulence and the oscillation is observed from the wavy vortex mode. Recent Takeda’s study has measured the precise spatiotemporal velocity fields by using the ultrasonic velocity profiler (UVP). On the other hand with a short annulus, we have various vortex modes generated due to the Ekman boundary layers occurred near the upper and lower solid boundaries. Each vortex mode also has the same spectrum transition such as wavy, modulated and soft turbulence modes. But the flow state seems much different from the infinite annulus. Present study measures the velocity profiles of the flow in TVF with a short annulus by using the UVP. We focus on not only the velocity profiles, but also the spectra in oscillation mode that occurs in different vortex modes. From the results, the spectra of the various oscillations are occurred with increase of Reynolds number, and seem different from those in the infinite annulus. This oscillation analysis would contribute to the basic knowledge of the mixing effect in solid-liquid flow when a microorganism in suspension is used as a bioreactor.
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