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Journal articles on the topic 'Somogyi-Nelson'

Author: Grafiati

Published: 10 September 2021

Last updated: 29 July 2025

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1

Upadhyaya, Mahesh K., and David W. Konesky. "Amino acid activity in the Nelson–Somogyi glucose assay." Canadian Journal of Botany 68, no. 7 (1990): 1479–81. http://dx.doi.org/10.1139/b90-187.

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The Nelson–Somogyi assay has been widely used to measure reducing sugars in plant research. We found that the L-amino acids cysteine, cystine, serine, tryptophan, and tyrosine (at 0.1 to 1 mM) react in the Nelson–Somogyi glucose assay, yielding high absorbance values. Alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, hydoxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, and valine showed little or no effect on color development. Manganese ions strongly inhibited color development in the presence of glucose, serine, cystine, and tryptophan

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2

Anggraini, Welly. "Pengaruh Ph Terhadap Aktivitas Enzim Kitinase dari Isolat Actinomycetes dengan Metode Somogyi-Nelson." Jurnal Ilmiah Pendidikan Fisika Al-Biruni 4, no. 2 (2015): 219. http://dx.doi.org/10.24042/jpifalbiruni.v4i2.94.

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The pH effect on chitinase enzyme activity of actinomycetes isolate by Somogyi-Nelson method has been done. Chitinase enzymes can be produced by chitinolytic micro organisms, actinomycetes. This actinomycetes is taken from Mangrove from Coastal Ringgung of Teluk Lampung Waters, and has been isolated in previous research. The actinomycetes isolates used were ANL-12, ANL-9, ANLd-2b-3, and ANL-4, with chitinolytic activity of 1.9 cm, 2.0 cm, 2.3 cm, and 5.0 cm. The ANL-4 isolate, which has the largest chitinolytic activity, was selected for the next process, the solidstate fermentationprocess (SS

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Anggraini, Welly. "Pengaruh Ph Terhadap Aktivitas Enzim Kitinase dari Isolat Actinomycetes dengan Metode Somogyi-Nelson." Jurnal Ilmiah Pendidikan Fisika Al-Biruni 4, no. 2 (2015): 219. https://doi.org/10.24042/jipfalbiruni.v4i2.94.

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The pH effect on chitinase enzyme activity of actinomycetes isolate by Somogyi-Nelson method has been done. Chitinase enzymes can be produced by chitinolytic micro organisms, actinomycetes. This actinomycetes is taken from Mangrove from Coastal Ringgung of Teluk Lampung Waters, and has been isolated in previous research. The actinomycetes isolates used were ANL-12, ANL-9, ANLd-2b-3, and ANL-4, with chitinolytic activity of 1.9 cm, 2.0 cm, 2.3 cm, and 5.0 cm. The ANL-4 isolate, which has the largest chitinolytic activity, was selected for the next process, the solidstate fermentationprocess (SS

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4

González Blair, Gloria Helena, and Óscar Fernando Castellanos Domínguez. "Alternatives for modifying the Somogyi-Nelson method for determining reducing sugars by using their thermical possibilities." Ingeniería e Investigación 23, no. 2 (2003): 5–17. http://dx.doi.org/10.15446/ing.investig.v23n2.14589.

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This article proposes modifying the Somogyi-Nelson method for determining reducing sugars, considering that sodium arsenate (one of its constituents) is a toxic substance which is tending to disappear from the market. The chemical principles used in the Somogyi-Nelson method for determining reducing sugars are explained, taking those protocols proposed by Hawk et al (1954), Collmer et al (1988), Castellanos (1995) and the Universidad Nacional’s Chemistry Department (adapted from Fischer’s protocol [1989]) as reference. The reactions of Somogyi and Nelson’s reagents are evaluated separately. Th

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5

Rahmawati, Febriana, and Devina Ingrid Anggraini. "AKTIVITAS EKSTRAK DAUN SELADA MERAH (Lactuca sativa var. crispa) SEBAGAI PENURUNAN KADAR GLUKOSA SECARA IN-VITRO." CENDEKIA EKSAKTA 9, no. 2 (2024): 60–67. https://doi.org/10.31942/ce.v9i2.12284.

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Diabetes melitus merupakan salah satu penyakit (kronis) berupa gangguan metabolik yang ditandai dengan kadar gula darah yang melebihi batas normal. Kontrol gula darah sangat penting untuk mencegah terjadinya komplikasi. Pemanfaatan bahan alam untuk menjaga kadar gula darah menjadi alternatif masyarakat. Kadar gula darah merupakan parameter kadar glukosa yang ada di dalam tubuh. Penelitian ini dilakukan untuk mengetahui potensi ekstrak daun selada merah (Latuca sativa var. crispa) sebagai penurunan kadar glukosa dan nilai EC50. Ekstrak daun selada merah (Latuca sativa var. crispa) diperoleh den

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6

Anisa Sopiani, Ratih Aryani, and Bertha Rusdi. "Skrining Aktivitas Antidiabetes pada Beberapa Ekstrak Menggunakan Metode Nelson-Somogyi." Bandung Conference Series: Pharmacy 4, no. 2 (2024): 593–99. http://dx.doi.org/10.29313/bcsp.v4i2.14592.

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Abstract. Diabetes mellitus is a chronic health condition characterized by high blood glucose levels due to impaired insulin production or function. With the increasing prevalence of diabetes in Indonesia, it encourages researchers to look for alternative treatments sourced from natural materials. One of the first steps is to screen the potential of natural compounds as antidiabetics. In this systematic literature review, the potential of natural ingredients as antidiabetic candidates was assessed in vitro using the Nelson Somogyi method. The results of the study found that some extracts conta

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AKIBA, Masanori, Keiro HIGUCHI, Shoji MOTOMIZU, and Yasutada SUZUKI. "Determination of Reducing Sugars by FIA —Automation of Somogyi-Nelson Method—." BUNSEKI KAGAKU 73, no. 9 (2024): 523–29. http://dx.doi.org/10.2116/bunsekikagaku.73.523.

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8

., Masfria, Syahputra H, Haro G, and Nasution L R. "Phenolic and Flavonoids Contributions to the Antioxidant, Antidiabetic, and Anticholesterol Activities of Eriobotrya japonica Fruit Extract: An In Vitro Analysis." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 14, no. 04 (2024): 955–60. https://doi.org/10.25258/ijddt.14.4.4.

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Eriobotrya japonica has been traditionally used for its medicinal properties. This study investigates its fruit ethanol extract's phenolic and flavonoids profiles and evaluates its antioxidant, antidiabetic, and anticholesterol activities. Eriobotrya japonica fruit ethanol extract was tested for total phenolic and flavonoid content using Folin-Ciocalteu and AlCl3 methods. The antioxidant capacity was assessed by DPPH and metal chelation assays. The antidiabetic action was assessed using α-glucosidase inhibition and the Nelson-Somogyi method, whereas the Liebermann-Burchard method was used to m

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9

Hidayah, Thauhidayatul. "The Effect of Saccharomyces cerevisiae Inoculum Concentration On Bioetanol Production From Cocoa Peel (Theobroma cacao L)." SainsTech Innovation Journal 1, no. 1 (2018): 6–15. http://dx.doi.org/10.37824/sij.v1i1.2018.18.

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This study aims to determine the effect of S. cerevisiae inoculum concentration on the ethanol content produced in the fermentation process of cocoa pods. The study design used a completely randomized design (CRD). Parameters observed were alcohol content, reducing sugar content and pH. The research was carried out in the laboratory scale and pilot scale. The laboratory scale consists of preliminary and major research. Preliminary research aims to find the optimum concentration of H2SO4 to produce the highest reducing sugar. The main research phase, namely hydrolysis using optimum H2SO4 from p

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Puspaningsih, Ni Nyoman Tri, Akhmaloka Akhmaloka, Sofyan Hadi, Y. Sri Wulan Manuhara, and Bambang Irawan. "ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI ESCHERICHIA COLI DH5A." Berkala Penelitian Hayati 4, no. 2 (1999): 61–67. http://dx.doi.org/10.23869/bphjbr.4.2.19992.

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Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert. Characterization of the recombinant DNA by Stu1 and EcoR1 restriction enzymes indicated nucleotide sequence of insert gene. Digestion by BamHI, Cla1, and EcoRV restriction enzymes showed only one band that assumed size of insert gene is about 1500 bp. Gene expression showed that amylase enzyme activity by using Somogyi-Nelson method was 88.0265 Unit. This activity was 10 percent higher than transformant cont

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11

Siregar, Sri Hilma. "ISOLASI ENZIM SELULASE DARI PANKREAS KEONG MAS." Photon: Jurnal Sain dan Kesehatan 1, no. 2 (2011): 63–67. http://dx.doi.org/10.37859/jp.v1i2.118.

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Extract the cellulose was prepared by the precipitation method using aceton 50% (v/v) and its protein content was analized by Biuret method. The sugar content as the result of the hydrolization of cellulose was determined by Nelson Somogyi method. After precipitation of the protein enzyme, centrifugation and freeze drying were applied and the specific activity of he enzyme cellulose extract was 0.1012 U/mg protein in optimal condition of pH 4.5 and temperature 45oC, where as the maximum velocity 32.94 μg/ml/min and KM value were 0.7279 mg/ml. Respectively the purification fold was 3.58 times a

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Sari, Nurmala. "The Use of Glucose Syrup as Product of Selulosa Hidrolyze from the Jackfruit Rags (Artocarpus heterophylus Lamk) as Sweetner on Candies Production from the Coconut Plam (Cocos Nucifera L)." Journal of Pharmaceutical And Sciences 2, no. 1 (2019): 17–23. http://dx.doi.org/10.36490/journal-jps.com.v2i1.12.

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Research on the influence of polysaccharides from the jackfruit rags (Artocarpus Heterophyllus Lamk) on the content of glucose syrups on hydrolyzing with HCl 30% has been done. A sample has got with simple random sampling. The cellulose was isolated from the seeds of rags. The cellulose was hydrolyzed by HCl 30% to produce glucose syrups. The content was analyzed by the Nelson-Somogyi method and calculated by regression analysis. The results of analysis show that the content of glucose syrups from the cellulose from the jackfruit rags were 2.47%.  Keywords: cellulose, jackfruit rag

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13

Cândico, J. P., A. P. P. Macedo, and Eduardo Galembeck. "Development and Evaluation of a Portable Spectrophotometer." Revista de Ensino de Bioquímica 17 (September 13, 2019): 113. http://dx.doi.org/10.16923/reb.v17i0.893.

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INTRODUCTION: Colorimetric methods, such as proteins and glucose quantification, and enzymatic assays are widely performed in biochemical laboratories employing spectrophotometer equipment. Even being present in most of the labs that serve undergraduate students, low cost and portable spectrophotometers can be a valuable tool in high schools and for field studies. OBJECTIVES: We have assembled and evaluated a portable spectrophotometer, inspired in some open projects freely available on the Internet. MATERIALS AND METHODS: The system was built using an RGB LED as a light source and a light det

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14

Sadeghi, Rohollah, Fang Fang, Yijing Shao, Nora Olsen, Bin Du, and Amy Hui-Mei Lin. "Eliminating protein interference when quantifying potato reducing sugars with the miniaturized Somogyi-Nelson assay." Food Chemistry 373 (March 2022): 131473. http://dx.doi.org/10.1016/j.foodchem.2021.131473.

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15

Green, Frederick, Carol A. Clausen, and Terry L. Highley. "Adaptation of the Nelson-Somogyi reducing-sugar assay to a microassay using microtiter plates." Analytical Biochemistry 182, no. 2 (1989): 197–99. http://dx.doi.org/10.1016/0003-2697(89)90578-2.

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16

Arenas, Thelma, Aurora Osorio, Luis David Ginez, Laura Camarena, and Sebastian Poggio. "Bacterial cell wall quantification by a modified low-volume Nelson–Somogyi method and its use with different sugars." Canadian Journal of Microbiology 68, no. 4 (2022): 295–302. http://dx.doi.org/10.1139/cjm-2021-0238.

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The study of peptidoglycan-binding proteins frequently requires in vitro binding assays, in which the isolated peptidoglycan used as a substrate must be carefully quantified. Here, we describe an easy and sensitive assay for peptidoglycan quantification based on a modified Nelson–Somogyi reducing sugar assay. We report the response of this assay to different common sugars and adapt its use to peptidoglycan samples subjected to acid hydrolysis. This method showed better sensitivity than the peptidoglycan quantification method based on the acid detection of diaminopimelic acid. The method descri

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17

SARI, NURMALA, and Mariany Razali. "Penetapan Kadar Glukosa Reduksi dari Sirup Glukosa Hasil Hidrolisa Selulosa dari Limbah Buah Mengkudu (Morinda Citrifolia L) dengan Asam Klorida." Journal of The Indonesian Society of Integrated Chemistry 13, no. 2 (2021): 98–104. http://dx.doi.org/10.22437/jisic.v13i2.14731.

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Pemanfaatan limbah buah mengkudu (Morinda Citrifolia L) merupakan salah satu solusi pengurangan limbah. Buah mengkudu pada umumnya bermanfaat sebagai antioksidan dan untuk mengobati beberapa penyakit seperti hipertensi, diabetes dan lainnya. Pemanfaatan mengkudu baru sebatas daging buahnya sedangkan bagian limbahnya belum dimanfaatkan secara optimal. Tujuan dilakukan penelitian yaitu untuk memanfaatkan selulosa dari limbah buah mengkudu untuk pembuatan sirup glukosa melalui proses hidrolisis dengan menggunakan HCl 30% serta menentukan kadar glukosa reduksinya. Sampel berupa selulosa yang diiso

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18

Gusakov, Alexander V., Elena G. Kondratyeva, and Arkady P. Sinitsyn. "Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities." International Journal of Analytical Chemistry 2011 (2011): 1–4. http://dx.doi.org/10.1155/2011/283658.

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The Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for reducing sugars are widely used in measurements of carbohydrase activities against different polysaccharides. Using twelve commercial enzyme preparations, the comparison of the NS and DNS assays in determination of cellulase,β-glucanase, xylanase, andβ-mannanase activities was carried out. When cellulase activities against CMC were measured, the DNS assay gave activity values, which were typically 40–50% higher than those obtained with the NS assay. In the analysis of the xylanase,β-mannanase, andβ-glucanase activities, the

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19

Sari, Nurmala. "IDENTIFIKASI ANALISIS KADAR KARBOHIDRAT DAN KADAR GULA REDUKSI METODE LUFF SCHOORL DARI HIDROLISIS SELULOSA LIMBAH MENGKUDU (Morinda citrifolia L.)." JURNAL KIMIA SAINTEK DAN PENDIDIKAN 7, no. 1 (2023): 41–45. http://dx.doi.org/10.51544/kimia.v7i1.3942.

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Dalam penentuan uji kadar karbohidrat , pada sampel limbah buah mengkudu dilakukan analisis, yaitu analisis secara kuantitatif dan kualitatif. Uji analisis yang sudah dilakukan dalam analisis hidrolisis selulosa limbah buah mengkudu secara kualitatif yaitu menggunakan uji benedict sehingga menghasilkan endapan merah bata dan secara kuantitatif menggunakan metode Nelson-Somogyi menghasilkan kadar gula reduksi sebsar 11.87%. Secara berkelanjutan dilakukan uji penentuan kadar karbohidrat dari hasil hidrolisis yaitu analisis karbohidrat total dan analisis gula reduksi dengan metode Luff Schoorl. A

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20

Luhurningtyas, Fania Putri, Nova Hasani, Melati Aprilliana, Deny Saputra, and Dewi Prayasanti. "PENGARUH KOMBINASI EKSTRAK DAUN ASHITABA (Angelica keiskei Ito.) DAN DAUN SUKUN (Artocarpus communis) TERHADAP KADAR GLUKOSA DAN KOLESTEROL SECARA IN VITRO MENGGUNAKAN METODE FOTOMETRI." FITOFARMAKA: Jurnal Ilmiah Farmasi 9, no. 1 (2019): 47–55. http://dx.doi.org/10.33751/jf.v9i1.1260.

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Penggunaan bahan herbal dalam bentuk jamu-jamuan pada pasien penyakit degeneratif adalah hal yang umum dijumpai. Daun ashitaba dan daun sukun digunakan secara tradisional untuk alternatif pada penderita diabetes dan kolesterol. Diperlukan penelitian secara ilmiah untuk membuktikan efektifitas daun ashitaba dan daun sukun tersebut. Tujuan penelitian ini adalah untuk mengetahui aktivitas penurunan kadar glukosa serta kolesterol dari kombinasi ekstrak daun ashitaba dan daun sukun dengan perbandingan massa tertentu in vitro. Metode yang digunakan untuk menguji kadar glukosa adalah metode Nelson-so

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Murwindra, Rosa, Saryono Sikumbang, Amir Awaliddin, and Amilia Linggawati. "PENGARUH SUHU TERHADAP PRODUKSI ASAM LEVULINAT DARI INULIN UMBI DAHLIA (Dahlia Sp.)." Photon: Jurnal Sain dan Kesehatan 7, no. 01 (2016): 127–35. http://dx.doi.org/10.37859/jp.v7i01.573.

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Dahlia is a floriculture plant with tuber that rich in inulin. Inulin is soluble in hot water and it will settle on cool temperature in alcohol. The inulin extraction to use hot water from dahlia tuber take from Bukittinggi of West Sumatra. Extraction result from 100 g dahlia tuber is 4% inulin (w/w dahlia tuber), such as inulin flour is white gray. As qualitative test with Seliwanoff and Nelson-Somogyi methods, that inulin positive contain of fructose and reducing sugar. This inulin can be converted into levulinic acid using acid catalyst at high temperature. Production of levulinic acid with

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Shao, Yijing, and Amy Hui-Mei Lin. "Improvement in the quantification of reducing sugars by miniaturizing the Somogyi-Nelson assay using a microtiter plate." Food Chemistry 240 (February 2018): 898–903. http://dx.doi.org/10.1016/j.foodchem.2017.07.083.

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Febriani, Yunisha, Boy Rahardjo Sidharta, and Fransiskus Sinung Pranata. "Produksi Bioetanol Pati Umbi Talas (Colocasia esculenta (L.) Schott) dengan Variasi Konsentrasi Inokulum dan Waktu Fermentasi Zymomonas mobilis." Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati 5, no. 2 (2020): 92. http://dx.doi.org/10.24002/biota.v5i2.2506.

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Bioetanol dapat diproduksi dari hasil fermentasi bahan baku yang mengandung karbohidrat. Umbi talas (Colocasia esculenta (L.) Schott) memiliki karbohidrat yang cukup tinggi yakni 23,7% sehingga dapat dimanfaatkan sebagai penghasil bioetanol. Zymomonas mobilis merupakan mikrobia yang dapat mengubah glukosa menjadi etanol. Penelitian ini bertujuan mengetahui konsentrasi inokulum dan waktu fermentasi yang paling optimal untuk menghasilkan bioetanol dari pati umbi talas. Umbi talas dipotong, dikeringkan dan dihancurkan lalu diayak sampai berbentuk tepung. Tepung talas dihidrolisis dengan larutan H

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24

STRANTZ, A. A., and E. A. ZOTTOLA. "Stability of Cornstarch-containing Polyethylene Films to Starch-degrading Enzymes." Journal of Food Protection 55, no. 9 (1992): 736–38. http://dx.doi.org/10.4315/0362-028x-55.9.736.

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Processes have been developed to incorporate cornstarch into plastics with the intent of increasing the rate of plastic biodegradation. The effect of starch-degrading enzymes on food-grade polyethylene film that contained 6% cornstarch (CSPE) was examined. Control polyethylene film with no added starch, CSPE and laboratory grade soluble starch were treated with α-amylase, β-amylase, or amyloglucosidase. Samples were removed periodically and were subjected to the Nelson-Somogyi method for the determination of reducing sugar content. Treatment with α-amylase and β-amylase released over 30% of th

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Rahman, Rozyanti. "Effect of Fermentation Time and Weight of Bread Yeast on Bioethanol Content from Glucose Hydrolysis of Cellulose Empty Bunches Palm Oil (Elaesis guineensis Jacq.) with HCl 30%." Journal of Chemical Natural Resources 4, no. 1 (2022): 74–81. http://dx.doi.org/10.32734/jcnar.v4i1.9361.

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Research about the effect of fermentation time and weight of yeast bread on bioethanol concentration from the fermentation of the glucose from hydrolysis of cellulose oil palm empty fruit bunches (Elaeis guineensis Jack ) with HCl 30% has been done. From the research, it found that oil palm empty fruit bunches contained cellulose of 24.1298 %. The cellulose was isolated from oil palm empty fruit bunches. It was hydrolyzed by HCl 30% to yield glucose and was analyzed by Nelson-Somogyi Method and the rate of the glucose was 17,1051 %. The fermentation of glucose used various periods of fermentat

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Zulhijja .S, Faradiba, Zainal Abidin, and Aulia Wati. "UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL BIJI ALPUKAT (Persea americana Mill.) SECARA IN VITRO DENGAN MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS." Makassar Pharmaceutical Science Journal (MPSJ) 1, no. 2 (2023): 57–65. https://doi.org/10.33096/mpsj.v1i2.75.

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Diabetes melitus merupakan penyakit metabolik yang ditandai dengan hiperglikemia, penyebabnya yaitu faktor resistensi insulin dan disfungsi sekresi insulin. Alpukat digunakan sebagai obat tradisional untuk mengobati berbagai macam penyakit, salah satunya adalah bagian biji buah alpukat yang mengandung senyawa alkaloid, polifenol, tanin, flavonoid, triterpenoid, kuinon, saponin, monoterpen dan seskuiterpen serta dapat menurunkan kadar glukosa dalam darah. Tujuan penelitian ini adalah untuk menentukan potensi antidiabetes ekstrak etanol biji alpukat (Persea americana Mill.) secara in vitro denga

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Sari, Eka, S. Syamsiah, Hary Sulistyo, and Muslikhin Hidayat. "BIODELIGNIFIKASI ENCENG GONDOK UNTUKMENINGKATKAN DIGESTIBILITAS PADA PROSESHIDROLISIS ENZIMATIK." Reaktor 17, no. 1 (2017): 54. http://dx.doi.org/10.14710/reaktor.17.1.54-60.

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Abstract Water hyacinth is one of lignocellulose that has potential to bioethanol feedstocks. This is due to the high content of cellulose and hemicellulose its rapid growth and the plant is abundant in nature. Bioconversion of water hyacinth begins with biodelignification process. This biodelignification aims to remove lignin and reduce crystallinity of cellulose and hemicellulose to increase its digestibility in enzymatic hydrolysis processes. The purpose of this study was to evaluate the effect of biodelignification of water hyacinth using fungi and the effect on the digestibility of water

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Al-kayyis, Hasanul Kiyan, and Hari Susanti. "Perbandingan Metode Somogyi-Nelson Dan Anthrone-Sulfat Pada Penetapan Kadar Gula Pereduksi Dalam Umbi Cilembu (Ipomea batatas L.)." Journal of Pharmaceutical Sciences and Community 13, no. 02 (2016): 81–89. http://dx.doi.org/10.24071/jpsc.2016.130206.

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Darliana, Ina. "BIODEGRADASI LIMBAH TANDAN KOSONG KELAPA SAWIT (Elaeis guineensis Jacq.) MENGGUNAKAN KONSORSIUM AKTERI PENGHASIL ENZIM SELULASE." Wanamukti: Jurnal Penelitian Kehutanan 23, no. 1 (2021): 1. http://dx.doi.org/10.35138/wanamukti.v23i1.174.

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This research was conducted to observe the consortium cellulose enzyme activity that bacterial comes from oil palm empty fruit bunch waste. The use of bacterial isolates derived from oil palm empty fruit bunch waste is expected to have a good effect, because the bacteria have been adapted to live in a waste environment that has a high concentration of organic matter. The purpose of this study was to determine the activity of cellulose enzyme consortium of bacteria from palm oil waste in the process of biodegradation of oil palm empty fruit bunch waste (Elaeis guineensis Jacq.). The method used

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Novembrianto, Rizka, and Muslikha Nourma Rhomadhoni. "PENENTUAN LAJU PEMBENTUKAN GULA REDUKSI ECENG GONDOK PADA PROSES HIDROLISIS KOMBINASI DENGAN BAKTERI SELULOLITIK." JURNAL ENVIROTEK 13, no. 2 (2021): 55–60. http://dx.doi.org/10.33005/envirotek.v13i2.129.

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Kehadiran eceng gondok dalam jumlah yang massif akan menjadi masalah untuk air permukaan. Namun eceng gondok juga memiliki kandungan selulosa yang bisa dikonversi menjadi gula reduksi sebagai bahan untuk pembuatan bioethanol. Penelitian ini bertujuan untuk menentukan hasil gula reduksi tahap hidrolisis kombinasi dengan bakteri selulolitik dan laju reaksi maksimumnya. Kebutuhan eceng gondok yang digunakan adalah substrat pada variasi 0,025; 0,057; 0,100; 0,161 dan 0,232 % (b/v). Segmen pretreatment menggunakan kapang Phanerochaete chrysosporium dan dilanjutkan hidrolisis secara kimia dengan per

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Endang Nurcahyani, Yuliana Permata Sari, Elsi Diana, Sumardi, Hardoko Insan Qudus, and Sri Wahyuningsih. "Analysis of reducing sugar levels of Cattleya sp. orchid plantlet after induction fusaric acid in vitro." World Journal of Advanced Research and Reviews 14, no. 2 (2022): 095–99. http://dx.doi.org/10.30574/wjarr.2022.14.2.0369.

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Cattleya sp. orchid is one type of orchid plant that is widely known in Indonesia. The queen of orchids is the nickname given to the Cattleya sp. orchid because of the elegant shape and color of the flowers of the Cattelya sp. orchid. This research was conducted to determine the level of reducing sugar produced by plantlets of Cattleya sp. after the addition of fusaric acid in in vitro media. Determination of reducing sugar content was carried out using the Nelson – Somogyi method. This study used a completely randomized design (CRD), with five levels of treatment and five replications for eac

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Sekatresna, Widiyanti, Abdi Dharma, and Periadnadi. "PRODUKSI DAN PENENTUAN KONDISI OPTIMUM ENZIM XILANASE Bacillus amyloliquefaciens FUKUMOTO PADA SUBSTRAT XILAN JERAMI." Jurnal Riset Kimia 2, no. 1 (2015): 74. http://dx.doi.org/10.25077/jrk.v2i1.115.

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ABSTRACT The production and determination of optimal condition of xylanase produced by Bacillus amyloliquefaciens on rice straw xylan were investigated in this study. The parameters to be observed were optimal conditions of pH, temperature, substrate concentration and incubation time. Xilanase activity was determined by measuring the amount of reducing sugar formed in the enzymatic reaction based on Somogyi Nelson method. Optimal conditions needed for the production of xylanase were at pH 7, temperature 27°C and six days of incubation time. While optimal conditions of xylanase action were reac

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Betaditya, Dika, Gumintang Ratna Ramadhan, Yovita Puri Subardjo, Fishka Diah Betari, and Ine Berlian Yustika. "Sugar Content and Consumption of Franchise Beverages as Risk Overweight Factors." Jurnal Ilmiah Kesehatan (JIKA) 4, no. 2 (2022): 193–201. http://dx.doi.org/10.36590/jika.v4i2.243.

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Nowadays, the franchise business is growing very rapidly, one of which is sweet beverages. The characteristics of the berverages have a high sugar content that exceeds the recommended limit for daily consumption, which is 6-12 g. Excess sugar consumption can lead to being overweight. This study aimed to know the sugar content and total calories contained in franchise beverages. This research was an observational study with descriptive analysis design. The sugar content was tested using the Nelson-Somogyi method and total calories were tested using a bomb calorimeter. The number of beverage sam

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Salvador, Carmen Amelia, Maira Rojas, Gabriela Jaramillo Kouperman, et al. "Búsqueda de bacterias con actividad EC 3.2.1.4 (endo-1,4- beta-glucanasa) en Eisenia foetida (Oligochaeta, Lumbricidae)." Revista Ecuatoriana de Medicina y Ciencias Biológicas 33, no. 1-2 (2017): 57. http://dx.doi.org/10.26807/remcb.v33i1-2.223.

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La lombriz de tierra, Eisenia foetida, es hospedero de numerosos microorganismos simbiontes, muchos de los cuales han sido identificados recientemente, así como se ha descrito su actividad enzimática. Estos microorganismos suelen presentar actividad enzimática de diferente tipo. Algunos podrían presentar interés industrial por sus potenciales aplicaciones. En el presente trabajo, se eligió identificar aquellos microorganismos que tienen actividad endo-β-1,4-glucanasa, y evaluar dicha actividad por el método de los azúcares reductores de Nelson-Somogyi. Dichos azúcares reductores fueron liberad

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Chalá, Carla Simone de Almeida, Arione da Silva Pereira, Angela Diniz Campos, Judith Viégas, and Adriana Tourinho Salamoni. "VARIABILIDADE GENÉTICA PARA TEOR DE AÇÚCARES REDUTORES EM BATATAS SILVESTRES QUE OCORREM NO SUL DO BRASIL." Ciência Rural 31, no. 1 (2001): 43–47. http://dx.doi.org/10.1590/s0103-84782001000100007.

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Açúcares redutores e matéria seca são características muito importantes em batata para processamento. Duas espécies de batata silvestre, Solanum commersonii, subespécies commersonii e malmeanum, e S. chacoense, subespécie muelleri, ocorrem no Sul do Brasil. Essas espécies são importantes para programas de melhoramento, pois apresentam variabilidade para diversas características importantes, tais como, tolerância à seca e geada, resistências a doenças e insetos e teor de matéria seca. Os objetivos deste trabalho foram verificar a variabilidade genética e estimar a herdabilidade para teor de açú

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Nasution, Emma Zaidar, and Sari Mutiara Ginting. "Production of Glucose Syrup Derived from Cassava Peel Starch Using Amylase Enzyme from Green Bean Seed Sprout Extract (Phaseolus radiatus L.)." Journal of Chemical Natural Resources 6, no. 1 (2024): 72–79. http://dx.doi.org/10.32734/jcnar.v6i1.16225.

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The production of glucose syrup derived from cassava peels starch using amylase enzyme from green bean seed sprout extract (Phaseolus radiatus L.) has been carried out by isolating the enzyme from the green bean seed sprouts extract then purified by precipitation using ammonium sulphate with the saturation of 60% (w/v). Amylase enzyme activity was determined using the Nelson Somogyi method. The research results obtained the activity of the amylase enzyme in the deposition of 60% ammonium sulphate at 12.22 U/mL. Amylase enzyme was applied as a hydrolyzed starch from cassava peels with a weight

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Anggarain, Devina Ingrid i., Eka Wisnu Kusuma, and Nurul Retno Murti. "Uji Aktivitas Antidiabetes Kombinasi Ekstrak Etanol Bunga Turi Merah (Sesbania grandiflora L.) dan Bunga Telang (Clitoria ternatea L.) secara In Vitro." Jurnal Farmasi Sains dan Terapan 9, no. 2 (2022): 53–59. http://dx.doi.org/10.33508/jfst.v9i2.3776.

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Diabetes melitus merupakan salah satu penyakit comorbid yang dapat memperparah kondisi pasien Covid-19. Terdapat berbagai pengobatan dari bahan alam yang dapat membantu dalam penurunan dan pengontrol kadar gula darah. Penelitian ini bertujuan untuk mengetahui adakah aktivitas antidiabetes kombinasi ekstrak etanol bunga telang (Clitoria ternatea L.) dan bunga turi merah (Sesbania grandiflora L.) serta mengetahui nilai aktivitas antidiabetes kombinasi ekstrak etanol bunga telang (Clitoria ternatea L.) dan bunga turi merah (Sesbania grandiflora L.) dibanding ekstrak tunggal. Penelitian dimulai de

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Breuil, C., and J. N. Saddler. "Comparison of the 3,5-dinitrosalicylic acid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity." Enzyme and Microbial Technology 7, no. 7 (1985): 327–32. http://dx.doi.org/10.1016/0141-0229(85)90111-5.

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Sukandar, Ukan, Achmad Ali Syamsuriputra, L. Lindawati, and Yadi Trusmiyadi. "Sakarifikasi pati ubi kayu menggunakan amilase Aspergillus niger ITB CC L74." Jurnal Teknik Kimia Indonesia 10, no. 1 (2018): 1. http://dx.doi.org/10.5614/jtki.2011.10.1.1.

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Saccharification of cassava starch using amilase Aspergillus niger ITB CC L74 Bioethanol is a derivative product from cassava that is thoroughly developed nowadays. One of the bioethanol research program of the Chemical Engineering Product Design and Development Research Group, Faculty of Industrial Technology, Institut Teknologi Bandung in the year of 2008 was to increase productivity and the performance of α-amylase and glucoamylase from Aspergillus niger ITB CC L74 for saccharification of cassava starch in bioethanol production. In conjunction with the optimum condition of saccharification

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Kim, Yeon-Kye, and Yoshiyuki Sakano. "Analyses of Reducing Sugars on a Thin-layer Chromatographic Plate with Modified Somogyi and Nelson Reagents, and with Copper Bicinchoninate." Bioscience, Biotechnology, and Biochemistry 60, no. 4 (1996): 594–97. http://dx.doi.org/10.1271/bbb.60.594.

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Ahmad, Ahyar, and Harningsih Karim. "Purification and Characterization of Amyloglucosidase Enzyme from the Thermophilic Endomycopsis fibuligera Using Sago Starch as a Substrate." Indonesian Journal of Chemistry 16, no. 3 (2018): 302. http://dx.doi.org/10.22146/ijc.21147.

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An investigation on purification and characterization of amyloglucosidase enzyme from Endomycopsis fibuligera by fermentation of sago starch has been carried out. This enzyme is inductive and can be produced by fermenting sago starch in a medium containing E. fibuligera. Crude enzyme was obtained by centrifuging the medium cultures containing E. fibuligera at 6,000 rpm for 20 min and then adding with 0.15 M acetate buffer (pH 5.0). Enzyme activity was determined using Somogyi-Nelson method by quantifying the released glucose from amyloglucosidase catalysis of starch (0.2%) as substrate. Prepur

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Wardatun, Sri, Ike Yulia, and Aditya Aprizayansyah. "KANDUNGAN FLAVONOID EKSTRAK METANOL DAN EKSTRAK ETIL ASETAT DAUN SUKUN (Artocarpus altilis (Park.) Fosberg) DAN AKTIVITASNYA TERHADAP PENURUNAN KADAR GLUKOSA SECARA IN VITRO." FITOFARMAKA: Jurnal Ilmiah Farmasi 6, no. 2 (2016): 52–63. http://dx.doi.org/10.33751/jf.v6i2.754.

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ABSTRAKDaun dan kulit pohon sukun atau Artocarpus altilis (Park.) Fosberg dikenal oleh masyarakat di beberapa daerah sebagai bahan obat untuk mengobati penyakit diabetes secara alami. Hasil-hasil penelitian menunjukan bahwa daun dan kulit pohon sukun mengandung senyawa-senyawa golongan flavonoid yang tinggi, yang diyakini mampu menurunkan kadar glukosa dalam darah sehingga dapat dikembangkan sebagai obat antidiabetes. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak daun sukun terhadap penurunan kadar glukosa dan korelasinya dengan kandungan flavonoid. Kadar glukosa diukur secara in

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Volkova, G. S., E. N. Sokolova, N. A. Fursova, T. V. Yuraskina, and E. M. Serba. "Development of the innovative biotechnological methods of the biological preparations production for feed purposes." IOP Conference Series: Earth and Environmental Science 1052, no. 1 (2022): 012016. http://dx.doi.org/10.1088/1755-1315/1052/1/012016.

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Abstract The aim of this study was the production of the biological preparations based on the hydrolyzed pea flour and the yeast biomass. The research objects were Saccharomyces cerevisiae Y-1218 yeast with glucoamylase activity, pea flour, and Celloviridin S20X and Amyloprotoorizin S20X enzyme preparations (EP). The specific growth rate of the yeast was determined by the calculation method, protein content – via Kjeldahl and Lowry methods, reducing substances content – by Somogyi-Nelson assay, the amine nitrogen content – by the formol titration method, the number of living yeast cells – by s

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Dzulhijjah, Rahmi, Mohd Sarli, and Dina Arafa Shabayek. "Identification Of Nutritional Content, Taxonomy and Processed Products Of Campolay Fruit (Pouteria Champeciana)." International Journal Scientific and Professional (IJ-ChiProf) 1, no. 1 (2022): 1–9. http://dx.doi.org/10.56988/chiprof.v1i1.1.

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Pouteria champeciana (campolay) is a type of tropical fruit grown in Indonesia. Though the nutritional content of the fruit has the potential as a processed food product. This study analyzed taxonomy, vitamin C, total carotene, total sugar content and minerals (Ca, Mg, K, Na) in campolay fruit and processing campolay fruit flesh into processed food products. This type of research is descriptive research. Campolay fruit obtained from Tarogong Kidul, Garut Regency, West Java. Analysis of vitamin C levels in campolay fruit flesh using the iodometric titration method, total carotene using spectrop

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Luhurningtyas, Fania Putri. "Parijoto Fruit Extract Nanoparticles As Glucose-Lowering Agent In Vitro." Jurnal Kesehatan Prima 14, no. 2 (2020): 75. http://dx.doi.org/10.32807/jkp.v14i2.276.

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Parijoto fruit is an herbal that is the potential to be used for the treatment of lowering blood glucose. The active compound in parijoto fruit can decrease glucose levels is flavonoids. The use of natural materials is limit because of their low solubility. It often failed in the clinical phase. The solution to this problem is that the parijoto fruit extract is formulated in the form of nanoparticles using chitosan and STPP as encapsulate materials. This study aims to determine the activity of nano chitosan parijoto fruit extract (NPFE) as a glucose-lowering agent by applying nanoparticle tech

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Rivadeneira, Joel Padilla, and Fidel Rey P. Nayve Jr. "Alkali Impregnation and Steam Explosion of Cogon Grass for Improved Enzymatic Saccharification." Materials Science Forum 1069 (August 31, 2022): 177–82. http://dx.doi.org/10.4028/p-l27jy0.

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Process parameters for the alkali-impregnation of cogon and its combination with steam explosion were evaluated and optimized using Response Surface Modeling (RSM). Dried cogon (10% w/v) was impregnated in diluted base (1, 2, 3% (w/w) NaOH) at different ranges of temperature (50, 85,120 °C) and time (15, 32.5, 50 min), and then subjected to enzymatic saccharification using 60 FPU/g Accelerase 1500TM. The concentration of reducing sugar released was measured using Nelson-Somogyi Colorimetric Method. The RSM showed that the optimum condition, predicting 10% Reducing Sugar Yield (RSY), was treatm

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Tjandra, Kezia Abib Yerah, Kartika Sari Dewi, Asrul Muhamad Fuad, and Trisanti Anindyawati. "Expression and characterization of Trichoderma reesei endoglucanase II in Pichia pastoris under the regulation of the GAP promoter." Indonesian Journal of Biotechnology 25, no. 2 (2020): 127. http://dx.doi.org/10.22146/ijbiotech.55604.

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Trichoderma reesei is known to be one of the organisms capable for producing various types of cellulase in high concentrations. Among these cellulases, the highest catalytic efficiency of endoglucanases II (EGII, EC 3.2.1.4) are considered important for industrial application. The characterization of the EGII is necessary since it is widely used in high-temperature reactions in the industries. In this study, the recombinant EGII protein was expressed in Pichia pastoris and it has a molecular mass of approximately 52 kDa. Recombinant EGII was purified using Ni-NTA affinity chromatography and ch

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Llacza Ladera, Henry Frans, Pedro Luis Castellanos Sánchez, and Patricia Meza Mendoza. "Evaluación del complejo enzimático celulasa en hongos nativos del Perú." Revista del Instituto de investigación de la Facultad de minas, metalurgia y ciencias geográficas 24, no. 48 (2021): 323–28. http://dx.doi.org/10.15381/iigeo.v24i48.21784.

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En el Perú información sobre el potencial biotecnológico de los hongos nativos para la degradación de la celulosa presenta escasos registros. Este trabajo tuvo como objetivo evaluar la actividad celulolítica de hongos aislados en los departamentos de Cajamarca, Lima, Junín y Huánuco. 289 especies de hongos fueron sometidos a reactivación después de un periodo de conservación de 7 años empleando papel filtro y caldo Czapeck, evaluados de manera semicuantitativa empleando la técnica de difusión radial en agar Czapeck Na-CMC para luego seleccionar cinco cepas que fueron identificadas morfológicam

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Lestari, Nurrisma Ika, and Dwi Umi Siswanti. "Physiological and Anatomical Responses of Red onion (Allium cepa L.) to Drought Stress after Biofertilizer Application." Jurnal Biodjati 9, no. 2 (2024): 359–72. https://doi.org/10.15575/biodjati.v9i2.38613.

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Red onion (Allium cepa) is a plant that requires sufficient water and is susceptible to drought stress. The use of biofertilizer is employed because it contains microorganisms that enhance nutrient availability and assist plant growth under abiotic stress conditions. This study aims to analyze the physiological and anatomical responses of red onion bulbs following the application of biofertilizer under drought stress. The doses of biofertilizer used were 0; 10;15; and 20 L/ha with field capacity levels of 25; 50; 75 and 100%. The parameters tested were bulb diameter, reducing sugar content num

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Endang, Nurcahyani, Permata Sari Yuliana, Diana Elsi, Sumardi, Insan Qudus Hardoko, and Wahyuningsih Sri. "Analysis of reducing sugar levels of Cattleya sp. orchid plantlet after induction fusaric acid in vitro." World Journal of Advanced Research and Reviews 14, no. 2 (2022): 095–99. https://doi.org/10.5281/zenodo.7186059.

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<em>Cattleya</em> sp. orchid is one type of orchid plant that is widely known in Indonesia. The queen of orchids is the nickname given to the <em>Cattleya</em> sp. orchid because of the elegant shape and color of the flowers of the <em>Cattelya</em> sp. orchid. This research was conducted to determine the level of reducing sugar produced by plantlets of <em>Cattleya</em> sp. after the addition of fusaric acid in <em>in vitro</em> media. Determination of reducing sugar content was carried out using the Nelson – Somogyi method. This study used a

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