Academic literature on the topic 'Sorting center for wild animals'

Background: The capuchin monkey (Sapajus apella) and the night monkey (Aotus azarae infulatus) are primates with broad distribution in South America. Sapajus apella is best-studied species in the genus Cebus, and is commonly found in captivity in parks, zoos, and sorting centers in Brazil. Species in the genus Aotus have a primitive appearance with large eye sockets, and their nocturnal habits are unique among the apes today. Because these two species belong to the same order, they have similar morphological characteristics. In this work, we conduct a comparative study of the forelimb musculature between these two species to produce data for use in veterinary procedures.Materials, Methods & Results: Three capuchin monkey and three night monkey specimens were used, two females and one male for each species. Specimens were acquired from the National Center for Primates under approval from the Animal Research (CEPAN/IEC/SVS/MS) No. 008/2010. Specimens were frozen and donated after death by natural causes to the LaPMA (Animal Morphology Research Laboratory) at the Federal Rural University of Amazonia (UFRA). After being thawed in running water, the animals were fixed by intramuscular injection with 10% formaldehyde in aqueous solution and dissected to display thoracic limb muscle groups. Dissections were performed using basic instrumentation by creating a medial incision from the carpal region to the axillary region. After incision, the skin was folded and muscles were separated in order to identify muscle groups. The nomenclature adopted was based on the veterinary anatomy, and books about human anatomy for comparison of forearm muscles. The muscles that compound the shoulder was the supraspinal and infra-spinal. The arm was composed by the teres major and minor, the triceps brachii (long head, lateral head, and medial head), brachialis, biceps brachii, tensor muscle of the antebrachial fascia, coracobrachialis, and anconeous. The forearm included the extensor carpi radialis longus, extensor carpi radialis brevis, extensor digitorus, extensor digiti minimum, extensor carpi ulnaris, abductor pollicis longus, extensor pollicis brevis, flexor carpi ulnaris, flexor digitorum profundus, flexor digitorum superficialis, flexor carpi radialis, flexor pollicis longus, extensor carpi radialis brevis, and brachioradialis muscles. In general, the two species have the same muscle groups, except for the absence of the teres minor and brachioradialis in Capuchin monkey, and the absence of the long head of the triceps brachii and extensor carpi radialis brevis at night monkey.Discussion: The forelimb musculature in the capuchin and night monkey is similar that described in the literature for both domestic and wild species such as coati, paca, and bush dogs, with little variation among the studied species. Regarding the muscles of the forearm, the capuchin monkey and the night monkey have muscular anatomy similar to that of humans, with some variations. The anatomical variations in common traits do not impede function. Interspecific variation is a common occurrence in nature, and can be explained by species divergence which occurs over many years, for various reasons. Knowledge of the comparative miology of the capuchin monkey and the night monkey is still nascent, thus future research is needed to better understand the structure of these animals, which can then serve as a basis for studies of other ethological aspects of the species biology.

Abstract Abstract 94 Somatic hypermutation (SHM) is a natural process that introduces point mutations into immunoglobulin (Ig) genes during antibody affinity maturation. During the first phase of SHM, the enzyme activation-induced cytidine deaminase (AID) converts cytosine (C) to uracil (U) to result in a U-G mismatch. Spontaneous U-G mismatches are normally corrected by high-fidelity DNA repair pathways. However, during the second phase of SHM, U-G mismatches are processed by low-fidelity DNA repair pathways to yield mutations. These second phase pathways are initiated by recognition of the uracil by uracil DNA glycosylase (UNG) and MSH2/MSH6. As a DNA mutator, AID poses a direct threat to genomic integrity, a notion supported by reports demonstrating that aberrant targeting of AID contributes to translocations and point mutations of proto-oncogenes associated with B cell malignancy. Transgenic mice with constitutive and ubiquitous expression of Aid (Aid-Tg) provide a unique system for investigating the poorly understood dynamics of Aid targeting and DNA repair. In these animals, development of B and T lymphocytes is indistinguishable from that of wild-type littermates. However, within 5 to 7 months, a subset of Aid-Tg mice develop monoclonal T cell lymphomas with high levels of SHM of the T cell receptor locus (Tcr), cMyc, Pim1, Cd4 and Cd5 genes. Interestingly, these animals do not develop B cell malignancies, suggesting that B cells have a specific mechanism for regulating Aid activity and/or high-fidelity versus low-fidelity DNA repair of non-Ig genes. To further investigate this mechanism, we crossed Aid-Tg mice with Ung−/−Msh2−/− double knockout mice. In the absence of Ung and Msh2, Aid-generated U-G mismatches are not repaired and are simply replicated, leaving a footprint of Aid targeting in the form of C/G to T/A transition mutations. Our plan was to compare Aid targeting (Aid-Tg Ung−/−Msh2−/−) and DNA repair (Aid-Tg) patterns between ‘normal' splenic B and T cells, prior to the development of genomic instability and malignant transformation. Splenic B and T cells from 4-month-old Aid-Tg and Aid-Tg Ung−/−Msh2−/− mice were obtained by fluorescence activated cell sorting. Genomic DNA was prepared and a ∼1-kilobase (kb) region spanning the first 1.5-kb downstream of the major promoter of cMyc, Pim1 and H2afx was PCR-amplified and sequenced (∼40 to 60-kb/gene). Surprisingly, mutation frequencies of all genes were found to be extremely low in all cell types (range, 8.4 × 10−6 to 7.8 × 10−5 mutations/bp) and well below the mutation frequencies of 4 × 10−4 to 9 × 10−4 mutations/bp we have previously seen for these genes in germinal center B cells from Ung−/−Msh2−/− mice. In fact, these mutation frequencies approach the background mutation frequency attributable to the sequencing procedure of 1.6 × 10−5 mutations/bp (previously determined from Aid−/− mice). Additional sequencing in the T cells also did not demonstrate any mutations in Cd4 or Tcrb, contrasting the high mutation frequencies found in tumors from Aid-Tg mice. To explore the possible cause of these unexpected results, we proceeded with further analysis of Aid expression in our system. Resting splenic B and T cells were obtained from wild-type and Aid-Tg mice and divided into one group that was used to make resting total cellular RNA and protein lysates and a second group that was activated ex vivo with lipopolysaccharide and IL4 (for B cells) or anti-CD3/CD28 beads and IL2 (for T cells). Real-time RT-PCR demonstrated high and equal expression of Aid transcript in resting and activated Aid-Tg B and T cells that was ∼3 to 4-fold higher than activated wild-type B cells. Analysis of Aid expression by Western blotting demonstrated that Aid protein is expressed at equal levels in activated wild-type and Aid-Tg B cells but is undetectable in resting wild-type B and T cells, resting Aid-Tg B and T cells, and activated Aid-Tg T cells. Thus, the Aid transgene is highly transcribed but likely fails to initiate SHM of known target genes in splenic B and T cells because Aid protein is absent. Our findings suggest that, in this model, B and T cells have an internal mechanism for negatively regulating Aid protein, preventing aberrant SHM and malignant transformation. Further investigation is needed to determine whether Aid protein is regulated at the level of mRNA translation or protein stability and how this mechanism breaks down during the pathogenesis of lymphoid malignancy. Disclosures: No relevant conflicts of interest to declare.

The presence of contaminated animals in wildlife rehabilitation centers poses a threat for both animals and humans that come into contact with them or the contaminated environment. The aim of this study was to assess the presence of Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), as well as studying the biofilm formation capacity of these isolates, Yersinia enterocolitica, Salmonella enterica and Campylobacter spp. in wild animals from a rehabilitation center. During a two-year period, feces were collected from animals that were admitted to a wildlife rehabilitation center (n=324 samples). The pathogens were isolated from 51 (15.7%) animals of different species of mammals, birds and reptiles. Forty isolates (12.3%) were identified as S. aureus, of these, 32 (9.9%) were identified as MRSA and 72.5% were able to form biofilm. Y. enterocolitica was found in five mammals (5.1%), three reptiles (21.43%) and two birds (0.94%). Salmonella and Campylobacter were isolated from one bird each (0.67% and 0.67%, respectively). A wide diversity of animal species in rehabilitation centers, including birds, mammals and reptiles, can carry MRSA and enterobacteria of one health concern and eliminate in the feces. The presence of these pathogens in the gastrointestinal tract of wild animals admitted to a wildlife rehabilitation center shows the importance of microbiological monitoring of animals at the time of their admission and reinforces the need for specific hygienic-sanitary care.

The Federal scientific Agroengineering center VIM has developed technical tools, algorithms and software for the intelligent automatic control system for milking animals “Stimul” on the “Herringbone” milking unit in three versions. The created system does not include automatic selection gates for effective management of zootechnical and veterinary services of animals. (Research purpose) The research purpose is in developing an intelligent machine for automatic sorting of animals for servicing and managing the herd according to specified characteristics. (Materials and methods) The article presents the development of control and management systems in dairy farming based on the conceptual principles of digital transformation. The digital control system is based on a multifunctional panel controller. The created control unit has a port for connecting to the RS 485 network and provides support for network functions via the Modbus Protocol. The programming of the control unit has been made in the SMLogix tool environment, which supports the FBD function block language. (Results and discussion) The article presents an intelligent machine for automatic sorting of animal flows for servicing and managing the herd according to specified characteristics with the unification of hardware, software modules and interface. The article describes the necessary parameters for the automatic remote animal identification system, the basic component of the control system of an intelligent machine for sorting animals according to specified characteristics. (Conclusions) The machine allows to automatically identify, sort and send animals to the specified areas for individual service.

AbstractWe have investigated the distribution of ON- and OFF-center responses in the lateral geniculate nucleus of ferrets with normal and abnormal retinal projections. Electrophysiological recordings in normal pigmented animals confirm previous studies on mustelids showing that ON-center responses are found in the anterior, inner parts of laminae A and AI and OFF-center responses in posterior, outer leaflets. In albino animals, lamina A displays normal patterns of ON/OFF segregation but in lamina AI, which receives an abnormal crossed retinal projection, no consistent patterns of segregation are found. Following monocular enucleation on the day of birth, the uncrossed projection in pigmented ferrets remains expanded across the LGN. Anatomically and physiologically, this projection is segregated into two leaflets: an anterior, inner ON-center leaflet and a posterior outer OFF-center leaflet. We conclude that the persistence of ON/OFF segregation, independent of geniculate location, suggests that self-sorting of retinal input is an important factor in generating the segregation.

Toxoplasmosis is a cosmopolitan zoonotic disease caused by Toxoplasma gondii, an intracellular protozoan. The main source of infection for humans is meat contaminated with tissue cysts, the main invasive form of the parasite. The muscle tissue of seropositive animals of the family Suidae, subfamily Sus (domestic pig, wild boar) are the most common sources of infections with Toxoplasma gondii. The aim of this study was to determine the prevalence of T. gondii infections in the meat of wild boars (Sus scrofa) based on measurements of T. gondii antibodies in the enzyme-linked immunosorbent assay (ELISA). One hundred samples of muscle tissue were obtained from wild boars hunted in the Game Breeding Center in north-eastern Poland. The animals were divided into three age groups: weaners (27), subadults (38) and adults (35). The prevalence of toxoplasmosis was very high in the analyzed population, and 71% of the animals were classified as seropositive in ELISA. Antibodies against T. gondii were detected in 62.9% of weaners, 73.6% of subadults and 74.2% of adult boars. The seroprevalence of T. gondii antibodies was significantly higher in the animals hunted in the Game Breeding Center in comparison with the national average determined by other authors. Such extensive spread of the parasite in the natural environment can be attributed to geographic location, landform, presence of waterbodies, local climate, the size of the wild boar population and the spread of castor bean ticks (Ixodes ricinus).

Ex situ management of wildlife provides a possibility for species maintenance, research development and environmental education. But as captive facilities are usually much smaller than the area occupied by a species in the natural habitat, cleaning might be insufficient, domestic species such as cats might be in close proximity, and wild animals in captivity are inevitably stressed, animals kept in these facilities are more at risk of acquiring parasitic infections. Therefore, an appropriate prophylactic protocol and consequent anti-parasitic therapy of affected animals are fundamental for any wildlife conservation center. This report describes the procedures adopted in a conservation center for wild felines in which four adult Jaguarundis were diagnosed with severe infection with gastrointestinal parasites and flea infestation. Two animals died and based on the necropsy findings and fecal examinations, infections with Ancylostoma caninum and Toxocara cati were diagnosed, as well as severe infestations with Ctenocephalides felis felis fleas, all common parasites of domestic cats. We discuss the applied therapy and the adopted environmental management, along with the importance of regular health assessments and a preventive medicine program for this species in captivity.

Because the Wild Animals Screening Center (CETAS) focuses intensely on restoration and maintenance of primate health and diversity in the Amazon region, detection of potential threats to animal health is crucial. In this view, neglected infections such as leptospirosis are of particular interest. In this report, we have studied the seroreactivity of anti-Leptospira antibodies from primate blood samples collected at CETAS between September 2011 and September 2012. Of the 14 animals investigated by the microscopic agglutination test using live antigens, five (35.7%) were reactive against serovars Celledoni (1), Hebdomadis (1), Icterohaemorrhagiae (2), and Sejroe (1). The results indicate that it is important to identify the contact of these animals with possible pathogens in order to ensure reintroduction of healthy animals into the wild and further understand the disease epidemiology in animals under captivity, gathering, and voluntary surrender of domestic captivity in Acre, Brazil.

ABSTRACT Rab22a is a member of the Rab family of small GTPases that localizes in the endocytic pathway. In CHO cells, expression of canine Rab22a (cRab22a) causes a dramatic enlargement of early endocytic compartments. We wondered whether transferrin recycling is altered in these cells. Expression of the wild-type protein and a GTP hydrolysis-deficient mutant led to the redistribution of transferrin receptor to large cRab22a-positive structures in the periphery of the cell and to a significant decrease in the plasma membrane receptor. Kinetic analysis of transferrin uptake indicates that internalization and early recycling were not affected by cRab22a expression. However, recycling from large cRab22a-positive compartments was strongly inhibited. A similar effect on transferrin transport was observed when human but not canine Rab22a was expressed in HeLa cells. After internalization for short periods of time (5 to 8 min) or at a reduced temperature (16°C), transferrin localized with endogenous Rab22a in small vesicles that did not tubulate with brefeldin A, suggesting that the endogenous protein is present in early/sorting endosomes. Rab22a depletion by small interfering RNA disorganized the perinuclear recycling center and strongly inhibited transferrin recycling. We speculate that Rab22a controls the transport of the transferrin receptor from sorting to recycling endosomes.

Very small embryonic-like stem cells (VSELs) represent a unique rare population of adult stem cells (SCs) sharing several structural, genetic, biochemical, and functional properties with embryonic SCs and have been identified in several adult murine and human tissues. However, rat bone marrow- (BM-) derived SCs closely resembling murine or human VSELs have not been described. Thus, we employed multi-instrumental flow cytometric approach including classical and imaging cytometry and we established that newly identified population of nonhematopoietic cells expressing CD106 (VCAM-I) antigen contains SCs with very small size, expressing markers of pluripotency (Oct-4A and Nanog) on both mRNA and protein levels that indicate VSEL population. Based on our experience in both murine and human VSEL isolation procedures by fluorescence-activated cell sorting (FACS), we also optimized sorting protocol for separation of CD45−/Lin−/CD106+rat BM-derived VSELs from wild type and eGFP-expressing rats, which are often used as donor animals for cell transplantations in regenerative studiesin vivo. Thus, this is a first study identifying multiantigenic phenotype and providing sorting protocols for isolation VSELs from rat BM tissue for further examining of their functional propertiesin vitroas well as regenerative capacity in distinctin vivorat models of tissue injury.

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The Centers for Wild Animals Screening (CETAS) on jurisdiction of the Brazilian Institute of Environment and Renewable Resources (IBAMA), are responsible for the receipt and disposal of wild animals that are victims of trafficking throughout the national territory. The CETAS of Serop?dica (CETAS-RJ) is the only place responsible for the receipt and disposal of wild animals that are victims of trafficking in the state of Rio de Janeiro. This study aimed to characterize the species of wildlife, seized or voluntarily surrendered, 2008-2014 in CETAS of Serop?dica ? Rio de Janeiro. The total effective entries in the Wild Animal Screening Center was 39.777 (thirty-nine thousand, seven hundred seventy-seven) animals during the years 2008 to 2014, with the annual average of the last 7 years, 5.682 entries. Among the animals received, 91,25% (36.295) were birds, 5,47% (2.177) reptiles, 3,14% (1.248) mammals, 0,13% (53) invertebrates; between crustaceans, insects and arachnids and 0,01% (4) indeterminate information from seizures, deliveries or redemptions. The seizure was the origin most represented during the study period, with 88,75% (35.302) animals and an average of 5.043 animal per year. The Environmental Police Command (CPAM), along with other battalions of military and Civilian Police were the most representative bodies in the fight against wildlife trafficking. Receipt of birds between the years, identified 24 orders, 54 families and 255species of birds. The order Passeriformes was the one that had the highest number of incoming animals (92,22%), having predominance of Thaupidae family with 85,54% (28.634), followed by family Icteridae 3,41% (1.143). The collared (Sporophila caerulescens), the Saffron Finch (Sicalis flaveola), the Crack-iron-true (Saltator similis), the Songbird (Sporophila angolensis), accounted for about 78,18% of passerines, 61,15% of the seized birds and 56,60% of total birds received in CETAS ? RJ, during the study period
Os Centros de Triagem de Animais Silvestres (CETAS), sob jurisdi??o do Instituto Brasileiro de Meio Ambiente e Recursos Renov?veis (IBAMA), s?o respons?veis pelo recebimento e destina??o dos animais silvestres que s?o v?timas do tr?fico em todo territ?rio Nacional. O CETAS de Serop?dica (CETAS-RJ), localizado na Floresta M?rio Xavier ? o ?nico local respons?vel pelo recebimento e destina??o dos animais silvestres que s?o v?timas do tr?fico no Estado do Rio de Janeiro. Objetivou-se caracterizar as esp?cies, da fauna silvestre, apreendidas ou entregues voluntariamente, de 2008 a 2014 no CETAS de Serop?dica ? Rio de Janeiro. O total de entradas efetivas no Centro de Triagem de Animais Silvestres foi de 39.777 (Trinta e nove mil, setecentos e setenta e sete) animais, durante os anos de 2008 a 2014, tendo como m?dia anual, dos ?ltimos 7 anos, 5.682 entradas. Dentre os animais recebidos, 91,25% (36.295) foram aves, 5,47% (2.177) r?pteis, 3,14% (1.248) mam?feros, 0,13% (53) invertebrados; entre crust?ceos, insetos e aracn?deos provenientes de apreens?es, entregas ou resgates. A apreens?o foi a proced?ncia com maior representa??o durante o per?odo estudado, com 88,75% (35.302) animais e uma m?dia de 5.043 animais por ano. O Comando de Pol?cia Ambiental (CPAM), juntamente com outros batalh?es da pol?cia militar e civil, foram os ?rg?os mais representativos no combate ao tr?fico de animais silvestres. O recebimento de aves entre 2008 e 2014 representou cerca de 91,25% (36.295) das entradas em rela??o ao total de todas as classes entre os anos, sendo identificadas 24 ordens, 54 fam?lias e 255 esp?cies de aves. A ordem Passeriformes foi a que teve o maior n?mero de animais recebidos (92,22%), havendo predomin?ncia das fam?lias, Thaupidae com 85,54% (28.634) e Icteridae 3,41% (1.143). O coleirinho (Sporophila caerulescens), o can?rio-da-terra-verdadeiro (Sicalis flaveola), o trinca-ferro-verdadeiro (Saltator similis), o curi? (Sporophila angolensis), o tiziu (Volatinia jacarina), o tico-tico (Zonotrichia capensis), corresponderam a cerca de 78,18% do total de Passeriformes, 61,15% apreendidos e 56,60% das aves recebidas no CETAS - RJ, no per?odo do estudo.