Journal articles on the topic 'SP Wood Technology'

The anthracological analyses of domestic and ceremonial contexts of proto-Jê archaeological sites in southern Brazil and Argentina have yielded data regarding landscape, fire technology, fuel economy, wood selection, and wood use from about 1200 to 250 years BP. The inhabitants of these sites built up the landscape that they occupied, actively constructing and experiencing their domestic and ceremonial places and possibly engaging in vegetation management practices. They gathered timber and firewood in the Araucaria Forest and in intensely modified areas covered by secondary vegetation. These practices likely included logging and gathering fallen deadwood. Our data indicate cultural selection of particular species. Inga sp., Jacaranda sp., and Araucaria angustifolia were probably selected because of the meaning of these woods in the cosmological dual system of proto-Jê societies. Bamboos and palm stems may have been used as kindling and for fire making. These results are an important contribution to our understanding of the proto-Jê occupation and the relationships that these groups maintained with their plant environment.

A rapid biodiversity evaluation of arboreal termite was carried out on the campus of Kano University of Science and Technology, Wudil. Three different trees which include Mangifera indica, Azadirachta indica and Khaya senegalensis were selected from three different sites (campus new site, premises of administrative offices and commercial area) on the campus and examined for termite activity, either arboreal nests or mud tubes which indicated infestation by the termites. Using a soft brush termite samples were collected into a labeled sampling bottle containing 10% formalin solution by cutting open the mud tubes and the nests with a forceps on the trees. A total of one hundred and twelve (112) individuals termites were samples were collected during the study belonging to five species: Coptotermes sp., Microtermes sp., Odontotermes sp., Amitermes sp. and Nutitermes sp. The Coptotermes sp. are the most abundant species both on the trees and the sites. Khaya senegalensis has the greatest diversity of the termite fauna as compared to other trees while campus new site (site A) also has greatest diversity. Coptotermes sp are insects of agricultural economic importance and feed on dead wood; therefore, they are insect pests of these trees and other building structures on the campus.

A rapid biodiversity evaluation of arboreal termite was carried out on the campus of Kano University of Science and Technology, Wudil. Three different trees which include Mangifera indica, Azadirachta indica and Khaya senegalensis were selected from three different sites (campus new site, premises of administrative offices and commercial area) on the campus and examined for termite activity, either arboreal nests or mud tubes which indicated infestation by the termites. Using a soft brush termite samples were collected into a labeled sampling bottle containing 10% formalin solution by cutting open the mud tubes and the nests with a forceps on the trees. A total of one hundred and twelve (112) individuals termites were samples were collected during the study belonging to five species: Coptotermes sp., Microtermes sp., Odontotermes sp., Amitermes sp. and Nutitermes sp. The Coptotermes sp. are the most abundant species both on the trees and the sites. Khaya senegalensis has the greatest diversity of the termite fauna as compared to other trees while campus new site (site A) also has greatest diversity. Coptotermes sp are insects of agricultural economic importance and feed on dead wood; therefore, they are insect pests of these trees and other building structures on the campus.

In the present study, three climbing edible roses (‘Crown Princess Margareta’, ‘Falstaff’ and ‘Brother Cadfael’) were evaluated for total vegetative growth and flowers production, besides correlations with soil microbiological and agrochemical parameters. The applied organic technology included a soil preparatory stage with three ameliorative plants (Sinapis alba L., Phacelia tanacetifolia L. and Tagetes patula L.) and two organic mulch variants (wood chips and wool) after plantation. In variant Sinapis and Tagetes, all three varieties of roses showed the highest values for parameters: total vegetative growth, number of shoots, average shoots length and flowers number. Significant positive correlations between biometric parameters and soil bacteria, phosphorus, mineral nitrogen, potassium (0-20 cm horizon) and humus (20-40 cm horizon) content were observed. Significant positive correlations between the number of flowers and the soil respiration coefficient, soil content in phosphorus, potassium, humus, total soluble salts and mineral nitrogen were noticed. Negative correlations with soil pH have been observed. Taking into account the data obtained in the two flowering stages in 2017, the average yield per hectare cultivated with ‘Crown Princess Margareta’ was 9.44 t petals/ha. For the ‘Falstaff’ and ‘Brother Cadafael’ cultivars, the yields were 7.54 and 5.62 t petals per hectare respectively.

In the context of industrialization, wood industry plays an important role as one of thepillar components in building Indonesia industry where 76.36% is supported by Meranti (Shorea Sp.).However, the increase of wood processing industries produces waste biomass in the form of largeamount sawdust. Hydrothermal treatment is an environmentally clean technology of convertingbiomass into coal-like solid called hydrochar using subcritical water. The purpose of this research isto determine the effect of temperature and water to biomass (b/w) ratio in the formation of hydrocharfrom Meranti sawdust. This research was carried out using a 250 mL hydrothermal reactor where amixture of Meranti sawdust and water heated at temperature variation of 240 and 300oC; b/w ratio1:20, 2:20, 3:20; initial pressure of 1.0 Mpa and holding time for 30 minutes. Hydrochar were thencharacterized in terms of yield, caloric value, proximate and ultimate analysis. Based on experimentalresult, 39-78% raw materials were converted into hydrochar where the highest yield was found ontemperature operation of 240oC and b/w ratio 2:20.

Baseball is a popular and very lucrative bat-and-ball sport that uses a wooden bat to score runs. We hypothesize that new design features for baseball bats will emerge from their shape optimization using parametric modeling and genetic algorithms. We converge the location of two points on bats made from maple (Acer sp.) and ash (Fraxinus sp.) wood that are associated with increased velocity of a ball rebounding off a bat: vibrational nodal points and the center of percussion (COP). Our modeling and optimization approach was able to reduce the distance between the nodal points and COP from 166.0 mm to 52.1 mm. This change was similar in both wood species and resulted from changes to the geometry of the bat, specifically shifting of the mass of the bat toward the center of the barrel and removing mass from the very end of the barrel. We conclude that the combination of parametric finite element modeling and optimization using genetic algorithms is a powerful tool for exploring virtual designs for baseball bats that are based on performance criteria and suggest that our designs could be realized in practice using subtractive manufacturing technology.

The wood processing industry produces a significant amount of wood waste. Biomass valorization through pyrolysis has the potential to increase the added value of wood wastes. Pyrolysis is an important thermochemical process that can produce solid, liquid, and gas products. This paper aims to review the pyrolysis of wood wastes from Indonesia, including teak wood (Tectona grandis), meranti (Shorea sp.), sengon (Paraserianthes falcataria (L) Nielsen), and rubberwood (Hevea brasiliensis). The review is based on an in-depth study of reliable literatures, statistical data from government agencies, and direct field observations. The results showed that pyrolysis could be a suitable process to increase the added value of wood waste. Currently, slow pyrolysis is the most feasible for Indonesia, with the main product of charcoal. The efficiency of the slow pyrolysis process can be increased by harvesting also liquid and gaseous products. The use of the main product of pyrolysis in the form of charcoal needs to be developed and diversified. Charcoal is not only used for fuel purposes but also as a potential soil improvement agent.

One of the main reasons for the decline and exhaustion of wood materials in old buildings is biological factors. In Yazd According to the position of buildings in hot and dry desert climate and the use of wood as an integral part of the building structure, This factors of damage to obvious and hidden that could threaten the stability of buildings. With respect to this, the several period laboratory and field research to identify the species and damage caused by biological agents work on timber structures Which mainly on 11 to 14 century AD buildings of Yazd damage has Was performed. In order to identify malicious insects, so far three type of Borer beetles the scientific name of the family Buprestidae Pseudocastalia aegyptiaca and Xylopertha reflexicauda and family Bostrichus capucinus Bostrychidae and three termite species with scientific names Anacanthotermes vagans (Hagen) family of Hodotermitidae, Amitermes sp. and Microcerotermes sp. The family Trmytyda has been identified in Yazd. The damage that these insects create many factors such as type of wood density, moisture content of wood depends on environmental conditions and location of the attack. According to climate of Yazd and construction technology, most of the structural damage, such as domes, arches and brick walls to show. These damages brought down the long-term structural strength and the instability in natural factors such as earthquakes, the structural imbalances and collapse of the buildings.Therefore the pathology of Integral component on timber structures brick in assessing the health and stability of structure in a desert climate of Yazd is very important and necessary. In this paper, by introducing the use of timber in 11th century to 14 century adobe structures in Yazd to review and evaluate the damage caused by the destruction timber structures Monuments By biological agents are paid.

In this study we compared the ability of conventional and field-emission scanning-electron-microscopy (FESEM) and energy-dispersive analysis of X-rays (EDX) to visualise and map inorganic nano and microparticles in Southern pine (Pinus sp.) treated with an aqueous dispersion of micronised copper-carbonate and iron oxide. Conventional SEM-EDX was able to detect areas of the wood microstructure that contained higher concentrations of copper and iron, but EDX maps were affected by drift and specimens suffered beam damage. The high brightness of the FESEM's electron beam at low accelerating voltages reduced beam damage and helped when mapping the distribution of copper and iron particles. The clarity of EDX maps was further improved by using drift-correction software and by mapping low energy X-rays. FESEM-EDX was able to resolve individual copper and iron microparticles. We conclude that FESEM-EDX shows promise as a means of resolving and mapping the distribution of inorganic metal particles in wood and that this may lead to greater use of the technology as interest in the treatment of wood with inorganic nano and microparticles grows.

Tannins from Myrcia splendens from the Amazon rainforest were used for the first time in the formulation of a urea-tannin-formaldehyde (UTF) resin. The objective was to evaluate the effect of different tannin proportions on the glue line performance in Pinus sp. woods, glued with UTF adhesive. The tannins were extracted in a water bath, using 1500 mL of water and 100g of dry bark (liquor/bark 15:1), with addition of 3% Na2SO3, at a temperature of 70 °C for three hours. The adhesives formulations were based on the gradual replacement of mass of the urea-formaldehyde (UF) adhesive by tannin powder, totalizing four compositions and one control treatment. UTF adhesives were evaluated for their physicochemical properties, shear bond strength (wet and dry) and wood failure. The addition of M. splendens tannins to the urea formaldehyde adhesive promoted an increase in glue line strength. The proportion of 5.0% of tannins proved to be ideal in the formulation of the UTF adhesive, considering all the parameters evaluated. Thus, this study proves the potential use of Amazonian tannins from Myrcia splendens in the partial composition of more sustainable UTF adhesives.

Pseudomonas sp. strain DY1 was newly isolated from soil with rotten wood and identified as a member of the genus Pseudomonas based on 16S rDNA and biochemical tests. Acid Black 172, a water soluble Cr-complex dye, was then selected as a model dye to investigate the decolorisation ability of the strain. It was observed that the growth of the strain was not inhibited by high dose of metal ions (10 mM), and efficient decolorisation was still observed when high concentrations of Fe2+, Fe3+ and Ca2+ existed. The optimal decolorising conditions obtained from Taguchi design were as follows: temperature 37˚C, pH 7.0, Fe3+ and proline concentrations of 7 mM and 3.0 g/L, respectively. Under the optimal conditions, 94.5% of Acid Black 172 (100 mg/L) could be decolorised by the strain in 24 h. The kinetics of the decolorisation best fitted the first order kinetic model (R2=0.981). Besides, the phytotoxicity study demonstrated a good detoxification by the strain. This work has a certain practical value in microbial decolorisation of textile wastewater.

Fractionation of lignocellulose into its three main components, lignin, hemicelluloses, and cellulose, is a common approach in modern biorefinery concepts. Whereas the valorization of hemicelluloses and cellulose sugars has been widely discussed in literature, lignin utilization is still challenging. Due to its high heterogeneity and complexity, as well as impurities from pulping, it is a challenging feedstock. However, being the most abundant source of renewable aromatics, it remains a promising resource. This work describes a fractionation procedure that aims at stepwise precipitating beech wood (Fagus sp.) lignin obtained with OrganoCat technology from a 2-methyltetrahydrofuran solution, using n-hexane and n-pentane as antisolvents. By consecutive antisolvent precipitation and filtration, lignin is fractionated and then characterized to elucidate the structure of the different fractions. This way, more defined and purified lignin fractions can be obtained. Narrowing down the complexity of lignin and separately valorizing the fractions might further increase the economic viability of biorefineries.

Nanhai No. 1, a shipwreck in the Southern Song Dynasty, China, has a history of more than 800 years. It was salvaged in 2007 and is now on display in the Guangdong Maritime Silk Road Museum. Due to the fact that the hull is a wooden cultural relic and exposed to the air, the biological corrosion and biodegradation caused by microorganisms are key problems of hull protection. At present, the antimicrobial agent Euxyl® K100 (isothiazolinone) has a significant antimicrobial effect in the field, but it has a certain negative impact on the environment and archeologists. In order to reduce the use of chemical antimicrobial agents, we evaluated the inhibitory effects of cinnamaldehyde on the main destructive microorganisms of Nanhai No. 1. Cinnamaldehyde is the main active component of cinnamon, and has broad-spectrum antimicrobial properties. The paper diffusion method, gas diffusion method and minimum inhibitory concentration experiment were used to detect the inhibitory effects of cinnamaldehyde on the main microorganisms of Nanhai No. 1. We found that cinnamaldehyde had significant inhibitory effects on Bacillus tequilensis NK-NH5, Bacillus megaterium NK-NH10, Bacillus velezensis NK-NH11, Bacillus sp. NK-NH15, Bacillus sp. NK-NH16, Bacillus sp. NK-NH17, Fusariumsolani NK-NH1 and Scedosporiumapiospermum NK.W1-3. At the same time, cinnamaldehyde had more inhibitory effects on fungi than bacteria. Finally, we verified that cinnamaldehyde can effectively inhibit the growth of microorganisms in water, for storing the scattered wood blocks of the Nanhai No. 1 hull through laboratory simulation experiments. Cinnamaldehyde, as an environment-friendly antimicrobial agent, is of great significance to protecting water-saturated wooden relics from microbial corrosion and degradation in the future.

This study is prompted by the concern over stagnant rates of creation in producing contemporary statues in Bali, both in the northern and southern regions, by using stone, wood, and metal mediums. The technology of using gips (plaster of Paris) is easier in statue production, however dismisses innovation in the process. The researcher took interest in I Wayan Sujana’s 10 years long research (2007-2017) on transferring of the unconscious onto art from novels (books). Based on that research I Wayan Sujana produced thousands of drawings with rich periodicity patterns. Those patterns are reviewed and selected to be made as contemporary statues. The production method for the contemporary statues, using Drawing Pattern on Novel, was participatory, involving traditional art carving experts. User Participation Method, an approach with user involvement in the art, judgment and creation methods by SP Gustami, was employed to conduct this study. The data was gathered with interviews, observation, documentation, and then exploration, planning and embodiment. This study aimed to create innovation of the fine arts, based on research, using Drawing Pattern on Novels, and can be recognized as part of Indonesian fine arts development. Indonesian contemporary fine arts focuses on local genius as the spirit of its creation. This research generated innovative statutes from stone with Indonesian national culture’s aesthetic motifs.

The mangrove ecosystem is one of the objects that can be identified by using remote sensing technology. The geographical location of the mangrove ecosystem located in the land and sea transition areas provides a distinctive recording effect when compared to other land vegetation objects. Remote sensing information about vegetation density is useful for various needs such as estimation of the availability of wood fuel biomass, succession stages, forest degradation and so on. This study aims to map the mangrove density using NDVI mangrove vegetation index from Landsat 8 image in Estuari Perancak, Jembrana, Bali. The study was conducted on August 20, 2016 until August 25, 2016. The analysis used is correlation analysis and t-Test analysis. Based on the results of the study, it was found that the density class was rare, medium and tight. The density class rarely has a pixel value range from 0.4765 to 0.6128, the medium density class has a pixel value range of 0.6128 to 0.7093, and the dense or dense density has a pixel value range of 0.7093 to 0.7947. The dominant mangrove species is Rhizopora sp. The linear regression equation in the above figure shows y = 0.679x + 0.438 and with the correlation (r) of 0.9642. This means that the density of mangroves and NDVI is directly proportional. Where the higher the value of mangrove density, the higher the value of NDVI and reserve.

Research into a constructed wetland for wastewater treatment using M.H.E.A. (Hierarchical Mosaic of Artificial Ecosystems) pilot system was carried out over a vegetative period in 8 different flow and vegetable composition series. The system consisted of a free water pond as a first step working as primary treatment followed by a zone with Typha sp. and surface flow and finally a woody zone with a subsurface flow and planted with ligneous species (Salix sp., Populus sp., Fraxinus sp. and Alnus sp.). Removal efficiency in the study reflects an optimal result: 80-99% total suspended matter removal, 82-98% organic matter removal, 70-98% nutrients removal and up to 99.9% faecal bacterial disinfecting. Effluent characteristics were in accordance with European Union legislation criteria for wastewater treatment systems.

Natural dyes had been used by human being since ancient times for colouring of various materials including textiles. These were replaced completely by synthetic dyes, continuous use of which created the problem of pollution and environmental degradation. With the development of green technology and increased awareness of sustainability, the use of non-allergic, non-toxic and eco-friendly natural dyes on textiles has become a matter of significant importance. Among the natural sources of colourants, microorganisms such as bacteria and fungi have gained interest in the field of textile coloration. In the present study, the pigment solution yielded from growth of Talaromyces purpurogenus sp. on organic waste was used for dyeing of wool fabric. Under the dyeing conditions i.e. 1:30 M:L ratio, pH 5, 80º C temperature and 45 minutes dyeing time, the percent absorption could reach 57 %, 32%, 36% and 49% respectively. The wool fabric samples dyed with the fungal dye exhibited very good to excellent washing and rubbing fastness properties. The colour pigment obtained from fungal thus offered an opportunity to reduce impact on plant resources for dye source exploration. So it can be concluded from the study that a natural fungal dye could help to sustain the environment and to minimize the over exploitation of natural resources.

Abstract The aim of this study was to evaluate the optimization of Nilasa red tilapia fish Oreochromis niloticus breeding process at Work Unit Freshwater Aquaculture (WUFA) Cangkringan, Technology Development Center of Fisheries (TDCOF) Yogyakarta. Nilasa red tilapia fish is one of the commodity that has advantages, including respond to artificial feed, grow fast, can live with high density and resistance to disease. WUFA Cangkringan was one of the government fisheries center which develop nilasa red tilapia fish. The hatchery activities is including maintenance of the parentstock, spawning, larval rearing, harvesting and post harvest. Nilasa red tilapia fish was a test fish that used at the WUFA Cangkringan from red tilapia study and released under the name Nilasa in 2012, start from 1st generation (F-0) to the 5th generation (F-4) in the program selection. The survival rate resulting from the hatchery activities with seed 2-3 cm was 80% and the seed was sold at Rp50 fish-1. The profit obtained Rp150 699 688 year-1, R/C ratio 1,34 and payback period 9 month. Key word: nilasa red tilapia fish, breeding process, survival rate

The popular historical narrative of the batik Sidoarjo needs to be reexamined based on historical methodology so that there is no historical bias based only on oral stories of the general public. Many studies are trapped in an inaccurate understanding of local historicity. As a result, these various studies have failed to fit batik Sidoarjo into its full context, instead it has become a kind of narrative standardization on its characteristics and history. This study aims to criticize the historical construction that has been popular in relation to the basic understanding of batik Sidoarjo and to explain the position of batik Sidoarjo in the cultural framework of its people. This article is the author's attempt to provide an analysis or explanation that is different from the historical narrative of batik Sidoarjo which is commonly used in various discussions. This research is classified as a qualitative research, using the historical method which consists of four stages, namely heuristics, source criticism, interpretation, and historiography. This research uses historical and sociological approaches to collect, select, and critically examine historical sources of Sidoarjo batik, resulting in historical facts. The results showed that the historicity of batik Sidoarjo refers to the batik activities in the areas of Kedungcangkring, Jetis, Sekardangan, Gajah Mada St. (Peranakans), and Tulangan, all of which have a direct relationship with both Peranakans nor indigenous. Batik Sidoarjo is not framed by traditional rituals, nor is it under the control and domination of the royal aristocracy. Its growth is based on the factor of the economic needs of the supporting community, which tends to be a trading commodity. The presence of other groups of people or nations such as Peranakan Chinese, Indo-European, Dutch, Arabic contributed to the birth of Sidoarjo batik. Keywords: batik, Sidoarjo, historical criticism.AbstrakNarasi sejarah batik Sidoarjo yang populer perlu dikaji ulang dengan didasari metodologi sejarah sehingga tidak terjadi bias sejarah yang hanya berdasar pada cerita lisan masyarakat umum. Banyak penelitian yang terjebak dalam pemahaman historisitas setempat yang kurang tepat. Akibatnya, berbagai kajian tersebut tidak berhasil mendudukkan batik Sidoarjo sesuai dengan konteksnya secara utuh, malah menjadi semacam standardisasi narasi pada karakteristik maupun sejarahnya. Penelitian ini bertujuan untuk mengkritisi konstruksi sejarah yang telah populer terkait pemahaman dasar tentang batik Sidoarjo serta menjelaskan kedudukan batik Sidoarjo dalam kerangka budaya masyarakatnya. Artikel ini merupakan upaya penulis untuk memberikan analisis atau paparan yang berbeda dari narasi sejarah batik Sidoarjo yang umum dilakukan pada berbagai pembahasan. Penelitian ini tergolong dalam penelitian kualitatif, dengan menggunakan metode sejarah yang terdiri atas empat tahap, yaitu heuristik, kritik sumber, interpretasi, dan historiografi. Penelitian ini menggunakan pendekatan historis dan sosiologis untuk mengumpulkan, menyeleksi, dan menguji secara kritis sumber-sumber sejarah batik Sidoarjo, sehingga menghasilkan fakta sejarah. Hasil penelitian memperlihatkan bahwa historisitas batik Sidoarjo merujuk pada aktivitas pembatikan yang ada di wilayah Kedungcangkring, Jetis, Sekardangan, Jl. Gajah Mada (China Peranakan), dan Tulangan yang kesemuanya saling terkait memiliki hubungan langsung baik itu pembatikan China peranakan maupun pribumi. Batik Sidoarjo tidak dikerangkai oleh ritual adat, juga tidak di bawah kendali dan dominasi aristokrasi kraton. Pertumbuhannya didasari faktor kebutuhan ekonomi masyarakat pendukungnya, sifatnya cenderung merupakan komoditas dagang. Hadirnya golongan masyarakat atau bangsa lain seperti China Peranakan, Indo-Eropa, Belanda, Arab turut berpengaruh melahirkan batik Sidoarjo.Kata Kunci: batik, Sidoarjo, kritik sejarah. Author:Asy Syams Elya Ahmad : Universitas Negeri Surabaya References:Abbas, Irwan. (2014). Memahami Metodologi Sejarah antara Teori dan Praktek. ETNOHISTORI: Jurnal Ilmiah Kebudayaan dan Kesejerahan, 1(1), 33–41.Abdurrahman, Dudung. (1999). Metode Penelitian Sejarah. Yogyakarta: Logos.Ahmad, Asy Syams Elya. (2013). Kajian Estetik Batik Sidoarjo. Tesis. Tidak Diterbitkan. Bandung: Program Studi Magister Desain, Institut Teknologi Bandung.Anas, Biranul, Hasanuddin, Ratna Panggabean, Yanyan Sunarya. (1997). Indonesia Indah-Buku ke 8; “Batik”. Jakarta: Yayasan Harapan Kita/BP 3 TMII.Anshori, Yusak & Kusrianto, Adi. (2011). Keeksotisan Batik Jawa Timur. Jakarta: Elex Media Komputindo.Anwarid. (2012). Geliat Batik Tulis Sidoarjo. Skripsi. Tidak Diterbitkan. Surabaya: Jurusan Pengembangan Masyarakat Islam, Fakultas Dakwah, Institut Agama Islam Negeri Sunan Ampel.Arfianti, D. Y., Afandi, A. F., permatasari, i., Agustin, F. R., & Nikmah, K. (2018). Batik Jetis Sidoarjo. https://doi.org/ 10.31227/osf.io/xq3r2 (diakses tanggal 17 April 2021).Benard, Russell H. (1994). Research Methods in Anthropology. London: Sage Publications.Carey, Peter. (1996). “The World of the Pasisir”, dalam Fabric of Enchantment; Batik from the North Coast of Java. County Museum of Art.Daliman. (2012). Metode Penelitian Sejarah. Yogyakarta: Ombak.Djoemena, Nian S. (1990a). Batik dan Mitra. Jakarta: Djambatan.________, Nian S. (1990b). Ungkapan Sehelai Batik: Its Mystery and Meaning. Cetakan II. Jakarta: Djambatan.Elliott, Inger McCabe. (2004). Batik, Fabled Cloth of Java. Singapore: Periplus.Fauzi, Ahmad. (2020, Juli 24). Daya Tarik Kampung Batik Jetis Sidoarjo. https://brisik.id/read/ 54889/daya-tarik-kampung-batik-jetis-sidoarjo (diakses tanggal 17 April 2021).Fitinline. (2013, Februari 17). Batik Sidoarjo. https://fitinline.com/article/ read/batik-sidoarjo/ (diakses tanggal 17 April 2021).Garraghan, Gilbert J. 1957. A Guide To Historical Method. New York: Fordham University Press.Gottschalk, Louis. (1975). Mengerti Sejarah. Terjemahan Nugroho Notosusanto. Jakarta: Yayasan Penerbit UI.Gray, Wood. (1964). Historian's Handbook: A Key to the Study and Writing of History. Boston: Houghton Mifflin.Gustami, SP. (2007). Butir-butir Estetika Timur; Ide Dasar Penciptaan Seni Kriya Indonesia. Yogyakarta: Prasista.Hani, Asfi. (2020, September 18). Sejarah Batik di Kampung Batik Jetis Sidoarjo. https://www. kompasiana.com/asfihani5098/5f642741097f3602e03e3cc3/sejarah-batik-di-kampung-batik-jetis-sidoarjo?page=all (diakses tanggal 17 April 2021).Hasanuddin. (2001). Batik Pesisiran: Melacak Etos Dagang Santri pada Ragam Hias Batik. Bandung: Kiblat.Harris, Jennifer, Ed. (1993). 5000 Years of Textiles. London: The British Museum Press.Hitchcock, Michael. (1991). Indonesian Textiles. Periplus Editions (HK) Ltd.Heringa, Rens & Veldhuisen, H.C. (1996). Fabric of Enchantment; Batik from the North Coast of Java. Los Angeles: County Museum of Art.Heringa, Rens. (2010). "Upland Tribe, Coastal Village, and Inland Court: Revised Parameters for Batik Research" dalam Five Centuries of Indonesian Textiles. Ruth Barnes & Mary Hunt Kahlenberg (Ed). Munich: Prestel.Irwanto, Dedi & Sair, Alian. (2014) Metodologi dan Historiografi Sejarah. Yogyakarta: EJA PUBLISHER.Irwantono, Yusuf & Hidayatun M.I. (2019). Fasilitas Wisata Edukasi Batik Sidoarjo di Sidoarjo. Jurnal eDIMENSI ARSITEKTUR, 7(1), 1089–1096. Ishwara, Helen, L.R. Supriyapto Yahya, Xenia Moeis. (2011). Batik Pesisir Pusaka Indonesia; Koleksi Hartono Sumarsono. Jakarta: KPG.Kartodirdjo, Sartono (1993). Pendekatan Ilmu Sosial dalam Metodologi Sejarah. Jakarta: Gramedia.Khasanah, Uswatun. (2018, Juni 8). Batik Asli Sidoarjo.https://doi.org/ 10.31227/ osf.io/zdka8 (diakses tanggal 17 April 2021).Kuntowijoyo. (2013). Pengantar Ilmu Sejarah. Yogyakarta: Tiara Wacana.Listanto, Virgiawan. (2019). “Batik Sebagai Representasi Produk Indsutri Kreatif di Sidoarjo Reinvensi Pragmatis untuk Inovasi Industri Kreatif Berbasis Budaya Visual Nusantara." Prosiding Seminar Nasional Seni dan Desain 2019, 465–469. Surabaya: Universitas Negeri Surabaya.Majlis, Brigitte Khan. (2000). “Javanesse Batik: An Introduction” dalam Rudolf G. Smend, Batik from The Courts of Java and Sumatra. Singapore: Periplus.Masadmin, (2016, Oktober 3). Batik Jetis Sidoarjo. Badan Perpustakaan dan Kearsipan Provinsi Jawa Timur. https:// jawatimuran.disperpusip. jatimprov.go.id/2016/10/03/batik-jetis-sidoarjo/ (diakses tanggal 17 April 2021).Maxwell, Robyn. (2003). Textiles of Southeast Asia: tradition, trade and transformation. Hongkong: Tuttle.Pranoto, Suhartono W. (2010). Teori dan Metodologi Sejarah. Yogyakarta: Graha Ilmu.Qamariah, Desti. (2012). Perkembangan Motif Batik Tulis Jetis Sidoarjo (2008-2011). Skripsi. Tidak Diterbitkan. Malang: Program Studi Pendidikan Sejarah, Fakultas Ilmu Sosial, Universitas Negeri Malang.Ran. (2015, Desember 5). Sempat Tenggelam, Kini Kian Eksis: Sejarah Panjang Batik Sidoarjo. Jawa Pos. https://www.pressreader.com/indone sia/jawa-pos/20151205/282656096383339 (diakses tanggal 17 April 2021).Ramadhan, Iwet. (2013). Cerita Batik. Tangerang: Literati.Rouffaer, G.P. & Juynboll, H.H. (1914). De Batikkunst in Nederlandsch Indië en haar geschiedenis. Utrecht: Oosthoek.Rusli. (2013). “Pendokumentasian Artifak Sejarah Pembatikan di Kedungcangkring”. Hasil Dokumentasi Pribadi: 2 Februari 2013. Kedungcangkring, Sidoarjo.Skocpol, Theda (ed.). (1984). Vision and Method in Historical Sociology. Cambridge: Cambridge University Press.Solikha, Rokhimatus. (2019). Sejarah Perkembangan dan Pengaruh Batik Jetis dalam Perekonomian Masyarakat Desa Jetis Sidoarjo. Skripsi. Tidak Diterbitkan. Surabaya: Program Studi Sejarah Peradaban Islam, Fakultas Adab dan Humaniora, Universitas Islam Negeri Sunan Ampel.Spradley, James. (1997). Metode Etnografi. Yogyakarta: Tiara Wacana.Susanto, Sewan. (1980). Seni Kerajinan Batik Indonesia. Jakarta: Balai Penelitian Batik dan Kerajinan. Lembaga Penelitian dan Pendidikan Industri, Departemen Perindustrian RI.Tjoa, Dave. (2004, Oktober 5). Batik Sidoarjo: Kampung Batik Jetis, Kampung Pengrajin Batik Tulis Sidoarjo. http://jejakbatik.blogspot. com/2014/10/batik-sidoarjo.html (diakses tang-gal 17 April 2021).Van Leur, J.C. (1955). Indonesian Trade and Society: Essay in Asean Social and Economical History. ‘s-Gravenhage: n.v. Uitgeverij W. Van Hoove.Van Roojen, Pepin. 2001. Batik Design. Amsterdam: Pepin Press.Wasino & Hartatik, Endah Sri. (2018). Metode Penelitian Sejarah: dari Riset hingga Penulisan. Yogyakarta: Magnum Pustaka Utama.Wibowo, Januar, Haryanto Tanuwijaya, Achmad Yanu A.F. (2016). “Rancang Bangun Management Information System Batik Tradisional Jawa Timur sebagai Upaya Pelestarian Warisan Budaya Bangsa”. Laporan Akhir Penelitian Hibah Bersaing. Tidak Diterbitkan. Surabaya: Institut Bisnis dan Informatika, STIKOM.Wirawan, Rizky S. & Trilaksana, Agus. (2015). Sejarah Industrialisasi Batik di Kampung Batik Jetis Sidoarjo Tahun 1970-2013. AVATARA, e-Journal Pendidikan Sejarah, 3(3), 480–486.Wulandari, Ari. (2011). Batik Nusantara; Makna Filosofis, Cara Pembuatan dan Industri Batik. Yogyakarta: Andi.Wulandari, S.E., Imam As’ary, Yudi Prasetyo. (2013). Perkembangan Motif Batik Jetis Sidoarjo dalam Tinjauan Sejarah. GENTA: Jurnal Pendidikan Sejarah, 1(1), 1–12.Yanuar. (2016, Oktober 19). Kampung Kuno Jetis Penghasil Batik Tulis Khas Sidoarjo. https://kabarinews.com/kampung-kuno-jetis-penghasil-batik-tulis-khas-sidoarjo/87296 (diakses tanggal 17 April 2021).

Cinchona alkaloids are known as antimalaria and anti-arrhythmic. Due to the long waiting time to harvest, cell culture technology is a challenge. This study aimed to determine the effects of elicitors, filtrate of two strains of endophytic fungi and methyl jasmonate (MeJA), in cell suspension culture of Cinchona ledgeriana on quinine and quinidine production. The cells were cultured for seven weeks in woody plant (WP) media treated with either of those elicitors in various concentrations. The cells growth was observed and the alkaloids were analyzed by HPLC. Cells treated with MeJA failed to grow that led to the cell biomass insufficiency for alkaloids determination. It indicates that the cells are quite sensitive to even low concentration of MeJA that hampered the growth. Cells treated with the filtrate of Diaporthe sp. M13-Millipore filtered (S2M) gave the least cell biomass but presented the highest content of both alkaloids. Diaporthe sp. strain M-13 is stronger as elicitor than M-23 for this plant species. Filtrate of non-virulent fungi can elevate the biosynthesis of alkaloids. This reconfirms that cultured cells are capable to produce secondary metabolites and the productivity can be increased by using an appropriate elicitor.

Currently, in Ukraine and abroad for the construction of high-rise buildings and structures using pipe-like structures. Wide application of pipe concrete columns is due to their high carrying capacity at relatively smaller overall dimensions due to the blocking of cracking in concrete by a steel clasp. The advantages of concrete columns should include more simplified conditions of technology of manufacturing and installation on their basis of bearing structures of floor covering. Piping constructions consist of steel shells and concrete core. Since the steel pipe mainly provides the bearing capacity of the concrete column, its failure or reduction of stiffness, which is characteristic of the fire under the influence of its thermal factors, leads to destruction. Investigation of fire resistance of concrete structures, which are not protected by flame retardant coatings, showed that a steel clasp during a fire after 15 minutes is heated to a critical temperature of 500 ° C.The use of flame retardant coatings is an effective method of fire protection of concrete constructions, which prevents the rapid heating of steel welds and provides a normalized fire resistance limit for such structures. In this work, studies were carried out on the effectiveness of fire protection of concrete columns with different types of fire-retardant materials - mineral wool slabs, special flame retardants and flame-retardant coatings. For fire protection mineral wool materials were used ROCKWOOL plates of the series "Conlit SL150". Mineral wool plates "Conlit SL 150" consist of fibers of rocks of a basalt group, they can withstand, without melting, temperature more than 1000 ° С. The silica-based adhesive "Conlit Glue" can withstand temperatures above 900 ° C, has good adhesion when bonding Conlit SL 150 mineral wool slabs with protective structures. From the second type of fire-retardant materials, the fire-proof composition "Naktresk" was chosen on the basis of gypsum. The coating is formed in the process due to hardening of the mixture on protected surfaces. The third type of flame retardant materials is the flame-retardant intumessent coating "Pyro-Safe Flammoplast SP-A2".It has been established that with the use of fire protection systems on the basis of mineral wool plates "Conlit SL150" and fire retardant "Nutresc", the fire resistance class of reinforced concrete columns increases from R 15 to R 180. The fire protection system on the basis of the painted paint "Pyro-Safe Flammoplast SP-A2" »Increases fire resistance from R 15 to R 75

The effect of foliar application of Cyanobacteria and Chlorella sp. monocultures on physiological activity, element composition, development and biomass weight of basket willow (Salix viminalis L.) and the possibility to prepare biofuel from it in the fortification process was studied. Triple foliar plant spraying with non-sonicated monocultures of Cyanobacteria (Anabaena sp. PCC 7120, Microcystis aeruginosa MKR 0105) and Chlorella sp. exhibited a considerably progressive impact on metabolic activity and development of plants. This biofertilization increased cytomembrane impermeability, the amount of chlorophyll in plants, photosynthesis productivity and transpiration, as well as degree of stomatal opening associated with a decreased concentration of intercellular CO2, in comparison to control (treatments with water, Bio-Algeen S90 or with environmental sample). The applied strains markedly increased the element content (N, P, K) in shoots and the productivity of crucial growth enzymes: alkaline or acid phosphorylase, total dehydrogenases, RNase and nitrate reductase. Treatments did not affect energy properties of the burnt plants. These physiological events were associated with the improved growth of willow plants, namely height, length and amount of all shoots and their freshly harvested dry mass, which were increased by over 25% compared to the controls. The effectiveness of these treatments depended on applied monoculture. The plant spraying with Microcystis aeruginosa MKR 0105 was a little more effective than treatment with Chlorella sp. and Anabaena sp. or the environmental sample. The research demonstrate that the studied Cyanobacteria and Chlorella sp. monocultures have prospective and useful potential in production of Salix viminalis L., which is the basic energy plant around the word. In this work, a special batch reactor was used to produce torrefaction material in an inert atmosphere: nitrogen, thermogravimetric analysis and DTA analysis, like Fourier-transform infrared spectroscopy. The combustion process of Salix viminalis L. with TG-MS analysis was conducted as well as study on a willow torrefaction process, obtaining 30% mass reduction with energy loss close to 10%. Comparing our research results to other types of biomasses, the isothermal temperature of 245 °C during thermo-chemical conversion of willow for the carbonized solid biofuel production from Salix viminalis L. biomass fertilized with Cyanobacteria and Chlorella sp. is relatively low. At the end, a SEM-EDS analysis of ash from torrefied Salix viminalis L. after carbonization process was conducted.

This study aimed to evaluate in vitro the pharmacological stability of a herbal mouthwash based on Libidibia ferrea extract after aging, establishing its organoleptic and microbiological characteristics. It is a 7.5% hydroalcoholic extract from Libidibia ferrea pods, using the reflux decoction technique, and spray dried. The stability, pH, sedimentation, density, and antimicrobial activity tests of the mouthwash were performed with the solutions stored after 24 months. Contaminant control was carried out by determining the total number of microorganisms and researching Salmonella sp., Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The formulation's antimicrobial activity against microorganisms present in the dental biofilm was analyzed: Streptococcus mutans, Streptococcus salivarius, Lactobacillus casei, and Candida albicans., The data were analyzed using the Tukey test to assess pH and density, and the other results were described using descriptive statistics., The color observed in the organoleptic characters, was “Ceramics”, with a fluid/serous consistency, shiny appearance, and a strong minty, woody odor. In the sedimentation phase, the separation was observed, and the pH and density analyzes showed a mean value of 5.46 and 1.029 g/cm3, respectively. The contaminant assessment test was negative for all microorganisms surveyed, within 24 months. The mouthwash showed bactericidal/fungicidal activity against all the microorganisms tested at all concentrations for S. mutans and C. albicans. At concentrations 0.5 mg/ml for S. salivarius and 0.6 mg/ml for L casei. It was concluded that after 24 months, the formulation of the mouthwash based on L. ferrea, its initial characteristics remained stable except for the homogeneity and pH of the solution.

Neste trabalho, objetivou-se analisar as transformações ocorridas no uso da linguagem por parte de seus usuários tendo como base o período correspondente ao início dos anos 90, momento histórico em que a internet ainda não havia sido popularizada no mundo, em comparação ao ano de 2017, período marcado pelo amplo acesso à internet, principalmente nos países mais desenvolvidos. Para tal, realizou-se uma investigação tendo como base o COCA (Corpus of Contemporary American English) com o intuito de se verificar, através da associação de palavras com seus colocados, como alguns termos eram utilizados antes da popularização da internet e após o mesmo fenômeno. Através da análise estatística dos insumos, foi possível identificar que certos termos da língua (neste caso da língua inglesa) passaram a ser utilizados mais frequentemente para expressar algo relacionado à tecnologia, tendo sido os sentidos anteriores rebaixados, nesta transformação semântica, a uma frequência menor ou muito menor de uso após a realidade do acesso amplo à internet, o que representa uma transformação léxico-semântica propiciada por um fenômeno de alcance global que influencia a vida das pessoas de modo a ressignificar o uso que fazem do mundo e consequentemente a metalinguagem que utilizam nas trocas que realizam com o mesmo.REFERÊNCIASBENSON, M., BENSON, E., ILSON, R. (orgs.)The BBI dictionary of english word combinations. Amsterdã/Filadélfia: John Benjamins, 1986.BIBER, D. Variation across speech and writing. Cambridge: Cambridge University Press, 1988Davies, Mark. The Corpus of Contemporary American English (COCA): 600 million words, 1990-present, 2008. Disponível em: https://www.english-corpora.org/coca/. Acesso em: 19 fev. 2020.CASTELLVI, Maria Teresa CABRÉ. La clasificación de neologismos. Alfa, São Paulo, 50 (2): 229-250, 2006DAVIES, Mark. The Corpus of Contemporary American English as the first reliable monitor corpus of English. Literary and Linguistic Computing, Brigham, v. 25, n. 4, 2010. Disponível em: https://academic.oup.com/dsh/article-abstract/25/4/447/997323?redirectedFrom=fulltext. Acesso em: 21 ago. 2019.FRANCIS, W. N.; KUCERA, H. Frequency analysis of English usage: lexicon and grammar. Boston: Houghton Mifflin, 1982DAVIES, Mark; KIM, Jong-Bok. Historical shifts with the INTO-CAUSATIVE construction in American English. The Gruyter mouton, [S.L.], v. 57, n. 1, 2019. Disponível em: http://web.khu.ac.kr/~jongbok/research/2019/2019-ahci-into-historical-shift-linguistics.pdf Acesso em 21 ago. 2019DICIONÁRIO PRIBERAM DA LÍNGUA PORTUGUESA. Desenvolvido por Lello editores, Porto, 1996 e 1999. Licensiado à Priberam em 2008. Disponível em: https://dicionario.priberam.org/sobre.aspx Acesso em 21 ago. 2019KJELLMER, G. A. A dictionary of English collocations: based on the Brown Corpus, v. 3. Oxford: Oxford University Press, 1994KREMELBERG, David. Practical statistic: a quick and easy guide to IBM ℗ SPSS ℗ Statistics, STATA, and other statistical software. Sage: Los Angeles, 2011.MC ENERY, Tony, et al. Corpus Linguistics, Learner Corpora, and SLA: Employing Technology to Analyze Language Use. Annual Review of Applied Linguistics (2019), 39, 74–92MODIS, Theodore. The end of the internet rush. Technological Forecasting Social Change, Lugano, v. 72, n. 8, 2005. Disponível em: https://www.sciencedirect.com/science/article/pii/S0040162505000843 Acesso em: 21 ago. 2019OLIVEIRA, Lúcia Pacheco de. Linguística de corpus: Teoria, interfaces e aplicações. Matraga, Rio de janeiro, v. 16, n. 24, 2009. Disponível em: https://www.e-publicacoes.uerj.br/index.php/matraga/article/view/27796. Acesso em: 21 ago. 2019PARTINGTON, A. Patterns and meanings: using corpora for english language research and teaching. Amsterdã/Filadélfia: John Benjamnins, 1998ROBINSON, Mary; DUNCAN, Daniel (2019) Holistic Approaches to Syntactic Variation: Wh-all Questions in English. University of Pennsylvania Working Papers in Linguistics: v. 25, n. 1 , 2019. Disponível em: https://repository.upenn.edu/pwpl/vol25/iss1/23/. Acesso em: 21 ago. 2019SANCHEZ, A. Definición e historia de los corpos. In: SANCHEZ, A. et al. (orgs.). CUMBRE: corpus linguístico de español contemporaneo. Madri: SGEL, 1995, p. 7-24.BERBER SARDINHA. T. Linguística de Corpus. Barueri, SP: Manole, 2004.SINCLAIR, J. McH. Beginning the study of lexis. In: BAZELL, C. E. In memory of R. Firth. Londres: Longman, 1966, p. 410-430.SVARTVIK, Jan. Corpora are becoming mainstream. In: THOMAS, J. and SHORT, M. (orgs). Using corpora for language research. London and New York: Longman,1996. p 3-13.Recebido em 16-11-2019 | Aceito em 12-02-2020

Environments containing significant concentration of NaCl such as salt lakes harbor extremophiles microorganisms which have a great biotechnology interest. To explore the diversity of Bacteria in Chott Tinsilt (Algeria), an isolation program was performed. Water samples were collected from the saltern during the pre-salt harvesting phase. This Chott is high in salt (22.47% (w/v). Seven halophiles Bacteria were selected for further characterization. The isolated strains were able to grow optimally in media with 10–25% (w/v) total salts. Molecular identification of the isolates was performed by sequencing the 16S rRNA gene. It showed that these cultured isolates included members belonging to the Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus genera with less than 98% of similarity with their closest phylogenetic relative. The halophilic bacterial isolates were also characterized for the production of biosurfactant and industrially important enzymes. Most isolates produced hydrolases and biosurfactants at high salt concentration. In fact, this is the first report on bacterial strains (A4 and B4) which were a good biosurfactant and coagulase producer at 20% and 25% ((w/v)) NaCl. In addition, the biosurfactant produced by the strain B4 at high salinity (25%) was also stable at high temperature (30-100°C) and high alkalinity (pH 11).Key word: Salt Lake, Bacteria, biosurfactant, Chott, halophiles, hydrolases, 16S rRNAINTRODUCTIONSaline lakes cover approximately 10% of the Earth’s surface area. The microbial populations of many hypersaline environments have already been studied in different geographical regions such as Great Salt Lake (USA), Dead Sea (Israel), Wadi Natrun Lake (Egypt), Lake Magadi (Kenya), Soda Lake (Antarctica) and Big Soda Lake and Mono Lake (California). Hypersaline regions differ from each other in terms of geographical location, salt concentration and chemical composition, which determine the nature of inhabitant microorganisms (Gupta et al., 2015). Then low taxonomic diversity is common to all these saline environments (Oren et al., 1993). Halophiles are found in nearly all major microbial clades, including prokaryotic (Bacteria and Archaea) and eukaryotic forms (DasSarma and Arora, 2001). They are classified as slight halophiles when they grow optimally at 0.2–0.85 M (2–5%) NaCl, as moderate halophiles when they grow at 0.85–3.4 M (5–20%) NaCl, and as extreme halophiles when they grow at 3.4–5.1 M (20–30%) NaCl. Hyper saline environments are inhabited by extremely halophilic and halotolerant microorganisms such as Halobacillus sp, Halobacterium sp., Haloarcula sp., Salinibacter ruber , Haloferax sp and Bacillus spp. (Solomon and Viswalingam, 2013). There is a tremendous demand for halophilic bacteria due to their biotechnological importance as sources of halophilic enzymes. Enzymes derived from halophiles are endowed with unique structural features and catalytic power to sustain the metabolic and physiological processes under high salt conditions. Some of these enzymes have been reported to be active and stable under more than one extreme condition (Karan and Khare, 2010). Applications are being considered in a range of industries such as food processing, washing, biosynthetic processes and environmental bioremediation. Halophilic proteases are widely used in the detergent and food industries (DasSarma and Arora, 2001). However, esterases and lipases have also been useful in laundry detergents for the removal of oil stains and are widely used as biocatalysts because of their ability to produce pure compounds. Likewise, amylases are used industrially in the first step of the production of high fructose corn syrup (hydrolysis of corn starch). They are also used in the textile industry in the de-sizing process and added to laundry detergents. Furthermore, for the environmental applications, the use of halophiles for bioremediation and biodegradation of various materials from industrial effluents to soil contaminants and accidental spills are being widely explored. In addition to enzymes, halophilic / halotolerants microorganisms living in saline environments, offer another potential applications in various fields of biotechnology like the production of biosurfactant. Biosurfactants are amphiphilic compounds synthesized from plants and microorganisms. They reduce surface tension and interfacial tension between individual molecules at the surface and interface respectively (Akbari et al., 2018). Comparing to the chemical surfactant, biosurfactant are promising alternative molecules due to their low toxicity, high biodegradability, environmental capability, mild production conditions, lower critical micelle concentration, higher selectivity, availability of resources and ability to function in wide ranges of pH, temperature and salinity (Rocha et al., 1992). They are used in various industries which include pharmaceuticals, petroleum, food, detergents, cosmetics, paints, paper products and water treatment (Akbari et al., 2018). The search for biosurfactants in extremophiles is particularly promising since these biomolecules can adapt and be stable in the harsh environments in which they are to be applied in biotechnology.OBJECTIVESEastern Algeria features numerous ecosystems including hypersaline environments, which are an important source of salt for food. The microbial diversity in Chott Tinsilt, a shallow Salt Lake with more than 200g/L salt concentration and a superficies of 2.154 Ha, has never yet been studied. The purpose of this research was to chemically analyse water samples collected from the Chott, isolate novel extremely or moderate halophilic Bacteria, and examine their phenotypic and phylogenetic characteristics with a view to screening for biosurfactants and enzymes of industrial interest.MATERIALS AND METHODSStudy area: The area is at 5 km of the Commune of Souk-Naâmane and 17 km in the South of the town of Aïn-Melila. This area skirts the trunk road 3 serving Constantine and Batna and the railway Constantine-Biskra. It is part the administrative jurisdiction of the Wilaya of Oum El Bouaghi. The Chott belongs to the wetlands of the High Plains of Constantine with a depth varying rather regularly without never exceeding 0.5 meter. Its length extends on 4 km with a width of 2.5 km (figure 1).Water samples and physico-chemical analysis: In February 2013, water samples were collected from various places at the Chott Tinsilt using Global Positioning System (GPS) coordinates of 35°53’14” N lat. and 06°28’44”E long. Samples were collected randomly in sterile polythene bags and transported immediately to the laboratory for isolation of halophilic microorganisms. All samples were treated within 24 h after collection. Temperature, pH and salinity were measured in situ using a multi-parameter probe (Hanna Instruments, Smithfield, RI, USA). The analytical methods used in this study to measure ions concentration (Ca2+, Mg2+, Fe2+, Na+, K+, Cl−, HCO3−, SO42−) were based on 4500-S-2 F standard methods described elsewhere (Association et al., 1920).Isolation of halophilic bacteria from water sample: The media (M1) used in the present study contain (g/L): 2.0 g of KCl, 100.0/200.0 g of NaCl, 1.0 g of MgSO4.7HO2, 3.0 g of Sodium Citrate, 0.36 g of MnCl2, 10.0 g of yeast extract and 15.0 g agar. The pH was adjusted to 8.0. Different dilutions of water samples were added to the above medium and incubated at 30°C during 2–7 days or more depending on growth. Appearance and growth of halophilic bacteria were monitored regularly. The growth was diluted 10 times and plated on complete medium agar (g/L): glucose 10.0; peptone 5.0; yeast extract 5.0; KH2PO4 5.0; agar 30.0; and NaCl 100.0/200.0. Resultant colonies were purified by repeated streaking on complete media agar. The pure cultures were preserved in 20% glycerol vials and stored at −80°C for long-term preservation.Biochemical characterisation of halophilic bacterial isolates: Bacterial isolates were studied for Gram’s reaction, cell morphology and pigmentation. Enzymatic assays (catalase, oxidase, nitrate reductase and urease), and assays for fermentation of lactose and mannitol were done as described by Smibert (1994).Optimization of growth conditions: Temperature, pH, and salt concentration were optimized for the growth of halophilic bacterial isolates. These growth parameters were studied quantitatively by growing the bacterial isolates in M1 medium with shaking at 200 rpm and measuring the cell density at 600 nm after 8 days of incubation. To study the effect of NaCl on the growth, bacterial isolates were inoculated on M1 medium supplemented with different concentration of NaCl: 1%-35% (w/v). The effect of pH on the growth of halophilic bacterial strains was studied by inoculating isolates on above described growth media containing NaCl and adjusted to acidic pH of 5 and 6 by using 1N HCl and alkaline pH of 8, 9, 10, 11 and 12 using 5N NaOH. The effect of temperature was studied by culturing the bacterial isolates in M1 medium at different temperatures of incubation (4°C–55°C).Screening of halophilic bacteria for hydrolytic enzymes: Hydrolase producing bacteria among the isolates were screened by plate assay on starch, tributyrin, gelatin and DNA agar plates respectively for amylase, lipase, protease and DNAse activities. Amylolytic activity of the cultures was screened on starch nutrient agar plates containing g/L: starch 10.0; peptone 5.0; yeast extract 3.0; agar 30.0; NaCl 100.0/250.0. The pH was 7.0. After incubation at 30 ºC for 7 days, the zone of clearance was determined by flooding the plates with iodine solution. The potential amylase producers were selected based on ratio of zone of clearance diameter to colony diameter. Lipase activity of the cultures was screened on tributyrin nutrient agar plates containing 1% (v/v) of tributyrin. Isolates that showed clear zones of tributyrin hydrolysis were identified as lipase producing bacteria. Proteolytic activity of the isolates was similarly screened on gelatin nutrient agar plates containing 10.0 g/L of gelatin. The isolates showing zones of gelatin clearance upon treatment with acidic mercuric chloride were selected and designated as protease producing bacteria. The presence of DNAse activity on plates was determined on DNAse test agar (BBL) containing 10%-25% (w/v) total salt. After incubation for 7days, the plates were flooded with 1N HCl solution. Clear halos around the colonies indicated DNAse activity (Jeffries et al., 1957).Milk clotting activity (coagulase activity) of the isolates was also determined following the procedure described (Berridge, 1952). Skim milk powder was reconstituted in 10 mM aqueous CaCl2 (pH 6.5) to a final concentration of 0.12 kg/L. Enzyme extracts were added at a rate of 0.1 mL per mL of milk. The coagulation point was determined by manual rotating of the test tube periodically, at short time intervals, and checking for visible clot formation.Screening of halophilic bacteria for biosurfactant production. Oil spread Assay: The Petridis base was filled with 50 mL of distilled water. On the water surface, 20μL of diesel and 10μl of culture were added respectively. The culture was introduced at different spots on the diesel, which is coated on the water surface. The occurrence of a clear zone was an indicator of positive result (Morikawa et al., 2000). The diameter of the oil expelling circles was measured by slide caliber (with a degree of accuracy of 0.02 mm).Surface tension and emulsification index (E24): Isolates were cultivated at 30 °C for 7 days on the enrichment medium containing 10-25% NaCl and diesel oil as the sole carbon source. The medium was centrifuged (7000 rpm for 20 min) and the surface tension of the cell-free culture broth was measured with a TS90000 surface tensiometer (Nima, Coventry, England) as a qualitative indicator of biosurfactant production. The culture broth was collected with a Pasteur pipette to remove the non-emulsified hydrocarbons. The emulsifying capacity was evaluated by an emulsification index (E24). The E24 of culture samples was determined by adding 2 mL of diesel oil to the same amount of culture, mixed for 2 min with a vortex, and allowed to stand for 24 h. E24 index is defined as the percentage of height of emulsified layer (mm) divided by the total height of the liquid column (mm).Biosurfactant stability studies : After growth on diesel oil as sole source of carbone, cultures supernatant obtained after centrifugation at 6,000 rpm for 15 min were considered as the source of crude biosurfactant. Its stability was determined by subjecting the culture supernatant to various temperature ranges (30, 40, 50, 60, 70, 80 and 100 °C) for 30 min then cooled to room temperature. Similarly, the effect of different pH (2–11) on the activity of the biosurfactant was tested. The activity of the biosurfactant was investigated by measuring the emulsification index (El-Sersy, 2012).Molecular identification of potential strains. DNA extraction and PCR amplification of 16S rDNA: Total cellular DNA was extracted from strains and purified as described by Sambrook et al. (1989). DNA was purified using Geneclean® Turbo (Q-BIO gene, Carlsbad, CA, USA) before use as a template in polymerase chain reaction (PCR) amplification. For the 16S rDNA gene sequence, the purified DNA was amplified using a universal primer set, forward primer (27f; 5′-AGA GTT TGA TCM TGG CTC AG) and a reverse primer (1492r; 5′-TAC GGY TAC CTT GTT ACG ACT T) (Lane, 1991). Agarose gel electrophoresis confirmed the amplification product as a 1400-bp DNA fragment.16S rDNA sequencing and Phylogenic analysis: Amplicons generated using primer pair 27f-1492r was sequenced using an automatic sequencer system at Macrogene Company (Seoul, Korea). The sequences were compared with those of the NCBI BLAST GenBank nucleotide sequence databases. Phylogenetic trees were constructed by the neighbor-joining method using MEGA version 5.05 software (Tamura et al., 2011). Bootstrap resembling analysis for 1,000 replicates was performed to estimate the confidence of tree topologies.Nucleotide sequence accession numbers: The nucleotide sequences reported in this work have been deposited in the EMBL Nucleotide Sequence Database. The accession numbers are represented in table 5.Statistics: All experiments were conducted in triplicates. Results were evaluated for statistical significance using ANOVA.RESULTSPhysico-chemical parameters of the collected water samples: The physicochemical properties of the collected water samples are reported in table 1. At the time of sampling, the temperature was 10.6°C and pH 7.89. The salinity of the sample, as determined in situ, was 224.70 g/L (22,47% (w/v)). Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions (table 1). SO4-2 and Mg+2 was present in much smaller amounts compared to Na +and Cl- concentration. Low levels of calcium, potassium and bicarbonate were also detected, often at less than 1 g/L.Characterization of isolates. Morphological and biochemical characteristic feature of halophilic bacterial isolates: Among 52 strains isolated from water of Chott Tinsilt, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for further characterization (table 2). The colour of the isolates varied from beige, pale yellow, yellowish and orange. The bacterial isolates A1, A2, A4, B1 and B5 were rod shaped and gram negative (except B5), whereas A3 and B4 were cocci and gram positive. All strains were oxidase and catalase positive except for B1. Nitrate reductase and urease activities were observed in all the bacterial isolates, except B4. All the bacterial isolates were negative for H2S formation. B5 was the only strain positive for mannitol fermentation (table 2).We isolated halophilic bacteria on growth medium with NaCl supplementation at pH 7 and temperature of 30°C. We studied the effect of NaCl, temperature and pH on the growth of bacterial isolates. All the isolates exhibited growth only in the presence of NaCl indicating that these strains are halophilic. The optimum growth of isolates A3 and B1 was observed in the presence of 10% NaCl, whereas it was 15% NaCl for A1, A2 and B5. A4 and B4 showed optimum growth in the presence of 20% and 25% NaCl respectively. A4, B4 and B5 strains can tolerate up to 35% NaCl.The isolate B1 showed growth in medium supplemented with 10% NaCl and pH range of 7–10. The optimum pH for the growth B1 was 9 and they did not show any detectable growth at or below pH 6 (table 2), which indicates the alkaliphilic nature of B1 isolate. The bacterial isolates A1, A2 and A4 exhibited growth in the range of pH 6–10, while A3 and B4 did not show any growth at pH greater than 8. The optimum pH for growth of all strains (except B1) was pH 7.0 (table 2). These results indicate that A1, A2, A3, A4, B4 and B5 are neutrophilic in nature. All the bacterial isolates exhibited optimal growth at 30°C and no detectable growth at 55°C. Also, detectable growth of isolates A1, A2 and A4 was observed at 4°C. However, none of the bacterial strains could grow below 4°C and above 50°C (table 2).Screening of the halophilic enzymes: To characterize the diversity of halophiles able to produce hydrolytic enzymes among the population of microorganisms inhabiting the hypersaline habitats of East Algeria (Chott Tinsilt), a screening was performed. As described in Materials and Methods, samples were plated on solid media containing 10%-25% (w/v) of total salts and different substrates for the detection of amylase, protease, lipase and DNAse activities. However, coagulase activity was determined in liquid medium using milk as substrate (figure 3). Distributions of hydrolytic activity among the isolates are summarized in table 4.From the seven bacterial isolates, four strains A1, A2, A4 and B5 showed combined hydrolytic activities. They were positive for gelatinase, lipase and coagulase. A3 strain showed gelatinase and lipase activities. DNAse activities were detected with A1, A4, B1 and B5 isolates. B4 presented lipase and coagulase activity. Surprisingly, no amylase activity was detected among all the isolates.Screening for biosurfactant producing isolates: Oil spread assay: The results showed that all the strains could produce notable (>4 cm diameter) oil expelling circles (ranging from 4.11 cm to 4.67 cm). The average diameter for strain B5 was 4.67 cm, significantly (P < 0.05) higher than for the other strains.Surface tension and emulsification index (E24): The assimilation of hydrocarbons as the sole sources of carbon by the isolate strains led to the production of biosurfactants indicated by the emulsification index and the lowering of the surface tension of cell-free supernatant. Based on rapid growth on media containing diesel oil as sole carbon source, the seven isolates were tested for biosurfactant production and emulsification activity. The obtained values of the surface tension measurements as well as the emulsification index (E24) are shown in table 3. The highest reduction of surface tension was achieved with B5 and A3 isolates with values of 25.3 mN m−1 and 28.1 mN m−1 respectively. The emulsifying capacity evaluated by the E24 emulsification index was highest in the culture of isolate B4 (78%), B5 (77%) and A3 (76%) as shown in table 3 and figure 2. These emulsions were stable even after 4 months. The bacteria with emulsification indices higher than 50 % and/or reduction in the surface tension (under 30 mN/m) have been defined as potential biosurfactant producers. Based on surface tension and the E24 index results, isolates B5, B4, A3 and A4 are the best candidates for biosurfactant production. It is important to note that, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was choosen for futher analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4.biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was chosen for further analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4. The biosurfactant produced by this strain was shown to be thermostable giving an E-24 Index value greater than 78% (figure 4A). Heating of the biosurfactant to 100 °C caused no significant effect on the biosurfactant performance. Therefore, the surface activity of the crude biosurfactant supernatant remained relatively stable to pH changes between pH 6 and 11. At pH 11, the value of E24 showed almost 76% activity, whereas below pH 6 the activity was decreased up to 40% (figure 4A). The decreases of the emulsification activity by decreasing the pH value from basic to an acidic region; may be due to partial precipitation of the biosurfactant. This result indicated that biosurfactant produced by strain B4 show higher stability at alkaline than in acidic conditions.Molecular identification and phylogenies of potential isolates: To identify halophilic bacterial isolates, the 16S rDNA gene was amplified using gene-specific primers. A PCR product of ≈ 1.3 kb was detected in all the seven isolates. The 16S rDNA amplicons of each bacterial isolate was sequenced on both strands using 27F and 1492R primers. The complete nucleotide sequence of 1336,1374, 1377,1313, 1305,1308 and 1273 bp sequences were obtained from A1, A2, A3, A4, B1, B4 and B5 isolates respectively, and subjected to BLAST analysis. The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus as shown in table 5. The halophilic isolates A2 and A4 showed 97% similarity with the Halomonas variabilis strain GSP3 (accession no. AY505527) and the Halomonas sp. M59 (accession no. AM229319), respectively. As for A1, it showed 96% similarity with the Halomonas venusta strain GSP24 (accession no. AY553074). B1 and B4 showed for their part 96% similarity with the Salinivibrio costicola subsp. alcaliphilus strain 18AG DSM4743 (accession no. NR_042255) and the Planococcus citreus (accession no. JX122551), respectively. The bacterial isolate B5 showed 98% sequence similarity with the Halobacillus trueperi (accession no. HG931926), As for A3, it showed only 95% similarity with the Staphylococcus arlettae (accession no. KR047785). The 16S rDNA nucleotide sequences of all the seven halophilic bacterial strains have been submitted to the NCBI GenBank database under the accession number presented in table 5. The phylogenetic association of the isolates is shown in figure 5.DICUSSIONThe physicochemical properties of the collected water samples indicated that this water was relatively neutral (pH 7.89) similar to the Dead Sea and the Great Salt Lake (USA) and in contrast to the more basic lakes such as Lake Wadi Natrun (Egypt) (pH 11) and El Golea Salt Lake (Algeria) (pH 9). The salinity of the sample was 224.70 g/L (22,47% (w/v). This range of salinity (20-30%) for Chott Tinsilt is comparable to a number of well characterized hypersaline ecosystems including both natural and man-made habitats, such as the Great Salt Lake (USA) and solar salterns of Puerto Rico. Thus, Chott Tinsilt is a hypersaline environment, i.e. environments with salt concentrations well above that of seawater. Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions, as in most hypersaline ecosystems (with some exceptions such as the Dead Sea). These chemical water characteristics were consistent with the previously reported data in other hypersaline ecosystems (DasSarma and Arora, 2001; Oren, 2002; Hacěne et al., 2004). Among 52 strains isolated from this Chott, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for phenotypique, genotypique and phylogenetique characterization.The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus. Genera obtained in the present study are commonly occurring in various saline habitats across the globe. Staphylococci have the ability to grow in a wide range of salt concentrations (Graham and Wilkinson, 1992; Morikawa et al., 2009; Roohi et al., 2014). For example, in Pakistan, Staphylococcus strains were isolated from various salt samples during the study conducted by Roohi et al. (2014) and these results agreed with previous reports. Halomonas, halophilic and/or halotolerant Gram-negative bacteria are typically found in saline environments (Kim et al., 2013). The presence of Planococcus and Halobacillus has been reported in studies about hypersaline lakes; like La Sal del Rey (USA) (Phillips et al., 2012) and Great Salt Lake (Spring et al., 1996), respectively. The Salinivibrio costicola was a representative model for studies on osmoregulatory and other physiological mechanisms of moderately halophilic bacteria (Oren, 2006).However, it is interesting to note that all strains shared less than 98.7% identity (the usual species cut-off proposed by Yarza et al. (2014) with their closest phylogenetic relative, suggesting that they could be considered as new species. Phenotypic, genetic and phylogenetic analyses have been suggested for the complete identification of these strains. Theses bacterial strains were tested for the production of industrially important enzymes (Amylase, protease, lipase, DNAse and coagulase). These isolates are good candidates as sources of novel enzymes with biotechnological potential as they can be used in different industrial processes at high salt concentration (up to 25% NaCl for B4). Prominent amylase, lipase, protease and DNAase activities have been reported from different hypersaline environments across the globe; e.g., Spain (Sánchez‐Porro et al., 2003), Iran (Rohban et al., 2009), Tunisia (Baati et al., 2010) and India (Gupta et al., 2016). However, to the best of our knowledge, the coagulase activity has never been detected in extreme halophilic bacteria. Isolation and characterization of crude enzymes (especially coagulase) to investigate their properties and stability are in progress.The finding of novel enzymes with optimal activities at various ranges of salt concentrations is of great importance. Besides being intrinsically stable and active at high salt concentrations, halophilic and halotolerant enzymes offer great opportunities in biotechnological applications, such as environmental bioremediation (marine, oilfiel) and food processing. The bacterial isolates were also characterized for production of biosurfactants by oil-spread assay, measurement of surface tension and emulsification index (E24). There are few reports on biosurfactant producers in hypersaline environments and in recent years, there has been a greater increase in interest and importance in halophilic bacteria for biomolecules (Donio et al., 2013; Sarafin et al., 2014). Halophiles, which have a unique lipid composition, may have an important role to play as surface-active agents. The archae bacterial ether-linked phytanyl membrane lipid of the extremely halophilic bacteria has been shown to have surfactant properties (Post and Collins, 1982). Yakimov et al. (1995) reported the production of biosurfactant by a halotolerant Bacillus licheniformis strain BAS 50 which was able to produce a lipopeptide surfactant when cultured at salinities up to 13% NaCl. From solar salt, Halomonas sp. BS4 and Kocuria marina BS-15 were found to be able to produce biosurfactant when cultured at salinities of 8% and 10% NaCl respectively (Donio et al., 2013; Sarafin et al., 2014). In the present work, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% NaCl. To our knowledge, this is the first report on biosurfactant production by bacteria under such salt concentration. Biosurfactants have a wide variety of industrial and environmental applications (Akbari et al., 2018) but their applicability depends on their stability at different environmental conditions. The strain B4 which can produce biosurfactant at 25% NaCl showed good stability in alkaline pH and at a temperature range of 30°C-100°C. Due to the enormous utilization of biosurfactant in detergent manufacture the choice of alkaline biosurfactant is researched (Elazzazy et al., 2015). On the other hand, the interesting finding was the thermostability of the produced biosurfactant even after heat treatment (100°C for 30 min) which suggests the use of this biosurfactant in industries where heating is of a paramount importance (Khopade et al., 2012). To date, more attention has been focused on biosurfactant producing bacteria under extreme conditions for industrial and commercial usefulness. In fact, the biosurfactant produce by strain B4 have promising usefulness in pharmaceutical, cosmetics and food industries and for bioremediation in marine environment and Microbial enhanced oil recovery (MEOR) where the salinity, temperature and pH are high.CONCLUSIONThis is the first study on the culturable halophilic bacteria community inhabiting Chott Tinsilt in Eastern Algeria. Different genera of halotolerant bacteria with different phylogeneticaly characteristics have been isolated from this Chott. Culturing of bacteria and their molecular analysis provides an opportunity to have a wide range of cultured microorganisms from extreme habitats like hypersaline environments. Enzymes produced by halophilic bacteria show interesting properties like their ability to remain functional in extreme conditions, such as high temperatures, wide range of pH, and high salt concentrations. These enzymes have great economical potential in industrial, agricultural, chemical, pharmaceutical, and biotechnological applications. Thus, the halophiles isolated from Chott Tinsilt offer an important potential for application in microbial and enzyme biotechnology. In addition, these halo bacterial biosurfactants producers isolated from this Chott will help to develop more valuable eco-friendly products to the pharmacological and food industries and will be usefulness for bioremediation in marine environment and petroleum industry.ACKNOWLEDGMENTSOur thanks to Professor Abdelhamid Zoubir for proofreading the English composition of the present paper.CONFLICT OF INTERESTThe authors declare that they have no conflict of interest.Akbari, S., N. H. Abdurahman, R. M. Yunus, F. Fayaz and O. R. Alara, 2018. Biosurfactants—a new frontier for social and environmental safety: A mini review. Biotechnology research innovation, 2(1): 81-90.Association, A. P. H., A. W. W. Association, W. P. C. Federation and W. E. Federation, 1920. Standard methods for the examination of water and wastewater. American Public Health Association.Baati, H., R. Amdouni, N. Gharsallah, A. Sghir and E. Ammar, 2010. Isolation and characterization of moderately halophilic bacteria from tunisian solar saltern. Current microbiology, 60(3): 157-161.Berridge, N., 1952. Some observations on the determination of the activity of rennet. Analyst, 77(911): 57b-62.DasSarma, S. and P. Arora, 2001. Halophiles. Encyclopedia of life sciences. Nature publishishing group: 1-9.Donio, M. B. S., F. A. Ronica, V. T. Viji, S. Velmurugan, J. S. C. A. Jenifer, M. Michaelbabu, P. Dhar and T. Citarasu, 2013. Halomonas sp. Bs4, a biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance. SpringerPlus, 2(1): 149.El-Sersy, N. A., 2012. Plackett-burman design to optimize biosurfactant production by marine Bacillus subtilis n10. Roman biotechnol lett, 17(2): 7049-7064.Elazzazy, A. M., T. Abdelmoneim and O. Almaghrabi, 2015. Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi journal of biological Sciences, 22(4): 466-475.Graham, J. E. and B. Wilkinson, 1992. Staphylococcus aureus osmoregulation: Roles for choline, glycine betaine, proline, and taurine. Journal of bacteriology, 174(8): 2711-2716.Gupta, S., P. Sharma, K. Dev and A. Sourirajan, 2016. Halophilic bacteria of lunsu produce an array of industrially important enzymes with salt tolerant activity. Biochemistry research international, 1: 1-10.Gupta, S., P. Sharma, K. Dev, M. Srivastava and A. Sourirajan, 2015. A diverse group of halophilic bacteria exist in lunsu, a natural salt water body of Himachal Pradesh, India. SpringerPlus 4(1): 274.Hacěne, H., F. Rafa, N. Chebhouni, S. Boutaiba, T. Bhatnagar, J. C. Baratti and B. Ollivier, 2004. Biodiversity of prokaryotic microflora in el golea salt lake, Algerian Sahara. Journal of arid environments, 58(3): 273-284.Jeffries, C. D., D. F. Holtman and D. G. Guse, 1957. Rapid method for determining the activity of microorgan-isms on nucleic acids. Journal of bacteriology, 73(4): 590.Karan, R. and S. Khare, 2010. Purification and characterization of a solvent‐stable protease from Geomicrobium sp. Emb2. Environmental technology, 31(10): 1061-1072.Khopade, A., R. Biao, X. Liu, K. Mahadik, L. Zhang and C. Kokare, 2012. Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination, 3: 198-204.Kim, K. K., J.-S. Lee and D. A. Stevens, 2013. Microbiology and epidemiology of Halomonas species. Future microbiology, 8(12): 1559-1573.Lane, D., 1991. 16s/23s rRNA sequencing in nucleic acid techniques in bacterial systematics. Stackebrandt e., editor;, and goodfellow m., editor. Chichester, UK: John Wiley & Sons.Morikawa, K., R. L. Ohniwa, T. Ohta, Y. Tanaka, K. Takeyasu and T. Msadek, 2009. Adaptation beyond the stress response: Cell structure dynamics and population heterogeneity in Staphylococcus aureus. Microbes environments, 25: 75-82.Morikawa, M., Y. Hirata and T. J. B. e. B. A.-M. Imanaka, 2000. A study on the structure–function relationship of lipopeptide biosurfactants. Biochimica et biophysica acta, 1488(3): 211-218.Oren, A., 2002. Diversity of halophilic microorganisms: Environments, phylogeny, physiology, and applications. Journal of industrial microbiology biotechnology, 28(1): 56-63.Oren, A., 2006. Halophilic microorganisms and their environments. Springer science & business media.Oren, A., R. Vreeland and L. Hochstein, 1993. Ecology of extremely halophilic microorganisms. The biology of halophilic bacteria, 2(1): 1-8.Phillips, K., F. Zaidan, O. R. Elizondo and K. L. Lowe, 2012. Phenotypic characterization and 16s rDNA identification of culturable non-obligate halophilic bacterial communities from a hypersaline lake, la sal del rey, in extreme south texas (USA). Aquatic biosystems, 8(1): 1-5.Post, F. and N. Collins, 1982. A preliminary investigation of the membrane lipid of Halobacterium halobium as a food additive 1. Journal of food biochemistry, 6(1): 25-38.Rocha, C., F. San-Blas, G. San-Blas and L. Vierma, 1992. Biosurfactant production by two isolates of Pseudomonas aeruginosa. World Journal of microbiology biotechnology, 8(2): 125-128.Rohban, R., M. A. Amoozegar and A. Ventosa, 2009. Screening and isolation of halophilic bacteria producing extracellular hydrolyses from howz soltan lake, Iran. Journal of industrial microbiology biotechnology, 36(3): 333-340.Roohi, A., I. Ahmed, N. Khalid, M. Iqbal and M. Jamil, 2014. Isolation and phylogenetic identification of halotolerant/halophilic bacteria from the salt mines of Karak, Pakistan. International journal of agricultural and biology, 16: 564-570.Sambrook, J., E. F. Fritsch and T. Maniatis, 1989. Molecular cloning: A laboratory manual, 2nd edn. Cold spring harbor laboratory, cold spring harbor, New York.Sánchez‐Porro, C., S. Martin, E. Mellado and A. Ventosa, 2003. Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. Journal of applied microbiology, 94(2): 295-300.Sarafin, Y., M. B. S. Donio, S. Velmurugan, M. Michaelbabu and T. Citarasu, 2014. Kocuria marina bs-15 a biosurfactant producing halophilic bacteria isolated from solar salt works in India. Saudi journal of biological sciences, 21(6): 511-519.Smibert, R., 1994. Phenotypic characterization. In methods for general and molecular bacteriology. American society for microbiology: 611-651.Solomon, E. and K. J. I. Viswalingam, 2013. Isolation, characterization of halotolerant bacteria and its biotechnological potentials. International journal scientific research paper publication sites, 4: 1-7.Spring, S., W. Ludwig, M. Marquez, A. Ventosa and K.-H. Schleifer, 1996. Halobacillus gen. Nov., with descriptions of Halobacillus litoralis sp. Nov. and Halobacillus trueperi sp. Nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. Nov. International journal of systematic evolutionary microbiology, 46(2): 492-496.Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar, 2011. Mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular biology evolution, 28(10): 2731-2739.Yakimov, M. M., K. N. Timmis, V. Wray and H. L. Fredrickson, 1995. Characterization of a new lipopeptide surfactant produced by thermotolerant and halotolerant subsurface Bacillus licheniformis bas50. Applied and environmental microbiology, 61(5): 1706-1713.Yarza, P., P. Yilmaz, E. Pruesse, F. O. Glöckner, W. Ludwig, K.-H. Schleifer, W. B. Whitman, J. Euzéby, R. Amann and R. Rosselló-Móra, 2014. Uniting the classification of cultured and uncultured bacteria and archaea using 16s rRNA gene sequences. Nature reviews microbiology, 12(9): 635-645

Xylaria sp. BCC 1067 is a wood-decaying fungus which is capable of producing lignocellulolytic enzymes. Based on the results of a single-molecule real-time sequencing technology analysis, we present the first draft genome of Xylaria sp. BCC 1067, comprising 54.1 Mb with 12,112 protein-coding genes.

The research aims to examine the physical and mechanical properties of cement board from sago fiber waste (Metroxylon Sp) based on cement size and composition and determine the best ratio of cement and sago fiber (Metroxylon sp) and meet the standards of JIS A 5417: 1992. The study was conducted at the Laboratorium WoodWorkshop, Wood Processing, Wood Technology and PT. Duta Pertiwi Nusantara Pontianak. The method used was an experimental method in a factorial Randomized Complete Design factorial experiment of 2 x 3 with 3 replications and a total of 18 experiments. The factors used in sago fiber length (Factor A) and fiber (Factor B). The tests include physical properties and mechanical properties with a target density of 1 gr / cm³. The results showed that the average value of the physical properties of the cement board included density 0.9713 gr / cm³ - 1.2246 gr / cm³, moisture content 3.5054% - 3.9448%, water absorption 0.9746 % - 1.1318% and thick Development 0.8649% - 0.9892%. The mean mechanical properties of cement board include MOE 10,564,6646 kg / cm² - 35,475,7865 kg / cm² and MOR 7,5797 kg / cm² - 25,8554 kg / cm². The best research is in treatment (a1b2) with a ratio of cement and fiber (80:20) with a length of sago fiber 2 cm and meets the standards of JIS A 5417: 1992 with the of 23, with a physical property density value of 1.2198 gr / cm³, water content is 3.7401%, water absorption is 0.9944%, and thickness development is 0.9048%. The mechanical properties value of Modulus of Elasticity is 28439.1825 kg / cm² and Modulus of Rupture is 25.8554 kg / cm².Keyword: Cement Comparison, Fiber Length, Sago Fiber, Physical and Mechanical Propertie