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Dissertations / Theses on the topic 'Specialized metabolism'

Author: Grafiati

Published: 4 June 2021

Last updated: 7 July 2024

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1

Xie, Zhengzhi. "Investigation of Plant Specialized Metabolism (Secondary Metabolism) Using Metabolomic and Proteomic Approaches." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/195218.

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Specialized metabolism (secondary metabolism) in glandular trichomes of sweet basil (Ocimum basilicum L.) and accumulation of specialized metabolites (secondary metabolites) in rhizomes of turmeric (Curcuma longa L.) was investigated using proteomic and metabolomic approaches, respectively. In an effort to further clarify the regulation of metabolism in the glandular trichomes of sweet basil, we utilized a proteomics-based approach that applied MudPIT (multidimensional protein identification technology) and GeLC-MS/MS (gel enhanced LC-MS/MS) to protein samples from isolated trichomes of four d

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2

Fallon, Timothy Robert. "The evolution and specialized metabolism of beetle bioluminescence." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122840.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2019<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references.<br>Fireflies (Lampyridae) and certain other families of beetles including the American railroad worms (Phengodidae), Asian starworms (Rhagophthalmidae), and American click-beetles (Elateridae), produce light in a process known as bioluminescence. The bioluminescent systems of beetles, natively used for the purposes of mating communication and/or an aposematic warning signal, are now well understood and have been widel

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3

Casas, Maria I. "BIOCHEMICAL AND GENETIC CHARACTERIZATION OF SPECIALIZED FLAVONOID METABOLISM IN MAIZE." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1431071650.

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4

Jorge, Letícia Galhardo. "Desempenho fotossintético, perfil e atividade do óleo essencial de Xylopia aromatica (Lam.) Mart. nas fases vegetativa e reprodutiva no cerrado paulista." Botucatu, 2020. http://hdl.handle.net/11449/192182.

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Orientador: Carmen Silvia Fernandes Boaro<br>Resumo: Espécies vegetais são capazes de produzir diversidade de substâncias, que desempenham funções importantes para sua sobrevivência e adaptação ao ecossistema. O metabolismo primário, é essencial para o crescimento, desenvolvimento, maturação e reprodução de qualquer espécie. O metabolismo especializado, dependente do primário, é responsável por originar o óleo essencial, que são misturas de metabólitos especializados voláteis, representados principalmente por monoterpenos e sesquiterpenos. Cada espécie vegetal produz um óleo essencial de compo

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5

Aubry, Céline. "Towards combinatorial biosynthesis of pyrrolamide antibiotics in Streptomyces." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS245.

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Depuis plus de 80 ans, le métabolisme spécialisé nous fournit de nombreuses molécules utilisées en médecine, en particulier comme anti-infectieux. Aujourd’hui, avec l’augmentation mondiale de la résistance aux antimicrobiens, de nouveaux antibiotiques sont indispensables. Une des réponses à cette pénurie grave pourrait provenir de la biologie synthétique. Dans le domaine du métabolisme spécialisé, la biologie synthétique est utilisée en particulier pour la biosynthèse de métabolites non naturels. Parmi les métabolites spécialisés, les peptides non ribosomiques constituent une cible attrayante,

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6

Erland, Lauren Alexandra Elizabeth. "Enhancement of specialized metabolism, regeneration efficiency and biological activity in lavandin (Lavandula x intermedia cv 'Grosso')." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/52707.

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This study aimed to improve essential oil composition by modifying terpene production in Lavandula x intermedia cv Grosso via mutagenesis to more closely resemble the oil of the commercially valuable essential oil from L. angustifolia or the medicinally active essential oil from L. latifolia. Additionally this study aimed to identify genes that control essential oil production in lavenders, and to determine the effect of essential oil composition on biological activity, specifically insecticidal and insect repellent properties. This study resulted in an improved method for the efficient regene

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7

Muchlinski, Andrew Joseph. "Identification, Characterization, and Functional Analysis of Terpenoid Specialized Metabolism in Switchgrass (Panicum virgatum) and Carrot (Daucus carota)." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/102778.

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Plants produce a large number of specialized or secondary compounds that aid in their reproduction and protection against biotic and abiotic stress. In this work I investigated the metabolism and function of terpenes, the largest class of specialized metabolites, in switchgrass and carrot. Switchgrass (Panicum virgatum L.), a perennial C4 grass of the Tallgrass Prairie, represents an important species in natural and anthropogenic grasslands of North America. Its natural resilience to abiotic and biotic stress has made switchgrass a preferred bioenergy crop. I have investigated the metabolism o

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8

Missinou, Anani Amegan. "Specialized metabolism in Brassica napus : Characterization of phytochemical diversity, its genetic determinants, and its regulation by pathogen infection." Electronic Thesis or Diss., Rennes, Agrocampus Ouest, 2022. http://www.theses.fr/2022NSARC162.

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Le métabolisme spécialisé joue un rôle essentiel dans les interactions plante-environnement. Sa caractérisation chez les plantes cultivées représente un enjeu scientifique important. Au cours de ma thèse, j’ai travaillé à la caractérisation du métabolisme spécialisé et de ses déterminants génétiques chez Brassica napus, et j’ai étudié sa régulation en réponse à l’infection par Leptosphaeria maculans, agent causal du phoma. J’ai contribué au développement d’une méthode de profilage métabolique ciblé et identifié 36 glucosinolates (GLSs) foliaires/racinaires, 32 composés phénoliques (PHLs) folia

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9

Fernández, Moreno Josefina Patricia. "TOMATO FLESHY FRUIT QUALITY IMPROVEMENT: CHARACTERIZATION OF GENES AND GENOMIC REGIONS ASSOCIATED TO SPECIALIZED METABOLISM IN TOMATO FLESHY FRUIT." Doctoral thesis, Universitat Politècnica de València, 2016. http://hdl.handle.net/10251/55505.

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[EN] Until recently, the genetic improvement of tomato (Solanum lycopersicum) was focused in agronomic traits, such as yield and biotic or abiotic stresses; therefore the interest in tomato fruit quality is relatively new. The tomato fruit surface can be considered both an agronomic trait as well as a quality trait, because it has an effect on consumer impression in terms of color and glossiness but also it underlies the resistance/sensitivity to cracking or water loss with consequences on fruit manipulation (e.g. transport and processing). The cuticle is deposited over the cell wall surroundi

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10

Sohrabi, Reza. "Biochemical and Functional Characterization of Induced Terpene Formation in Arabidopsis Roots." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/74938.

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Plants have evolved a variety of constitutive and induced chemical defense mechanisms against biotic stress. Emission of volatile compounds from plants facilitates interactions with both beneficial and pathogenic organisms. However, knowledge of the chemical defense in roots is still limited. In this study, we have examined the root-specific biosynthesis and function of volatile terpenes in the model plant Arabidopsis. When infected with the root rot pathogen Pythium irregulare, Arabidopsis roots release the acyclic C11-homoterpene (E)-4,8-dimethylnona-1,3,7-triene (DMNT), which is a common co

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11

Razmilic, Neira Valeria Isabel. "Metabolism analysis of streptomyces leeuwenhoekii C34 with a genome scale model and identification of Biosynthetic genes of specialized metabolites by genome mining." Tesis, Universidad de Chile, 2017. http://repositorio.uchile.cl/handle/2250/144111.

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Doctor en Ciencias de la Ingeniería, Mención Ingeniería Química y Biotecnología<br>Streptomyces leeuwenhoekii C34 es una nueva cepa que fue aislada desde la laguna Chaxa ubicada en el Desierto de Atacama, Chile. Esta cepa produce metabolitos especializados con actividad contra Staph. aureus resistente a meticilina (MRSA): chaxamicinas y chaxalactinas. La secuencia genómica de S. leeuwenhoekii C34 se obtuvo mediante las tecnologías de Illumina Miseq y PACbio RS II SMRT. El genoma se utilizó para identificar clústers de genes biosintéticos (BGCs) que codifican para metabolitos especializado

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12

Buges, Julie. "Metabolism and roles of hydroxycinnamic acids in Cichorium intybus L." Electronic Thesis or Diss., Université de Lille (2022-....), 2022. http://www.theses.fr/2022ULILR005.

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La chicorée industrielle, Cichorium intybus L. var. sativum, accumule une grandediversité de métabolites spécialisés. Ces métabolites montrent une grande variété destructures, activités et fonctions qui sont considérables d’un point de vue physiologique, mais aussi pour des applications humaines telle qu’en santé humaine et animale, en agronomie et autres diverses industries.Ce travail se concentre sur deux métabolites spécialisés de C. intybus : une phénolamide inhabituelle, la tétracoumaroyl-spermine (TetraSpm), et l’acide isochlorogénique (3,5-diCQA). Il constitue une contribution aux carac

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13

Najah, Soumaya. "Etude du métabolisme spécialisé de Streptomyces sp. TN58." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS496.

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Le nombre des génomes bactériens séquencés disponibles dans les bases des données ne cesse d’augmenter. Grâce au développement d’outils bio informatiques, l’exploration de ces données génomiques est devenue beaucoup plus aisée. Ces analyses génomiques révèlent qu’un important réservoir de gènes du métabolisme spécialisé, et potentiellement de métabolites bioactifs, reste encore à explorer. J’ai étudié le métabolisme spécialisé d’une souche de Streptomyces appelée Streptomyces sp.TN58, isolée à partir d’un échantillon de sol tunisien et retenue pour son spectre d’activité biologique assez large

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14

Huh, Jung-Hyun. "Biochemical, Molecular and Functional Analysis of Volatile Terpene Formation in Arabidopsis Roots." Diss., Virginia Tech, 2011. http://hdl.handle.net/10919/77151.

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Plants produce secondary (or specialized) metabolites to respond to a variety of environmental changes and threats. Especially, volatile compounds released by plants facilitate short and long distance interaction with both beneficial and harmful organisms. Comparatively little is known about the organization and role of specialized metabolism in root tissues. In this study, we have investigated the root-specific formation and function of volatile terpenes in the model plant Arabidopsis. As one objective, we have characterized the two root-specific terpene synthases, TPS22 and TPS25. Both en

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15

Weisberg, Alexandra Jamie. "Investigations into the molecular evolution of plant terpene, alkaloid, and urushiol biosynthetic enzymes." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/64408.

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Plants produce a vast number of low-molecular-weight chemicals (so called secondary or specialized metabolites) that confer a selective advantage to the plant, such as defense against herbivory or protection from changing environmental conditions. Many of these specialized metabolites are used for their medicinal properties, as lead compounds in drug discovery, or to impart our food with different tastes and scents. These chemicals are produced by various pathways of enzyme-mediated reactions in plant cells. It is suspected that enzymes in plant specialized metabolism evolved from those in pri

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16

Chalvin, Camille. "Sclareol biosynthesis in clary sage and its regulation." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS194/document.

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Le sclaréol est un diterpène produit par les organes floraux de la sauge sclarée (Salvia sclarea, Lamiaceae). Il est utilisé en parfumerie pour l’hémisynthèse de l’ambroxide, une substance caractérisée par une odeur ambrée et une grande capacité de fixation des parfums. L’augmentation de la demande mondiale en sclaréol stimule actuellement les tentatives d’accroître le rendement de la production de sclaréol à partir de la sauge sclarée. L’objectif du travail présenté dans ce manuscrit était d’améliorer notre compréhension de la biosynthèse du sclaréol et de sa régulation chez la sauge sclarée,

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17

Martinelli, Laure Marie Bernadette. "Étude de la biosynthèse des terpènes et de leur régulation chez Pelargonium x hybridum." Thesis, Lyon, 2020. http://www.theses.fr/2020LYSES010.

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Le genre Pelargonium fait partie de la famille des Geraniaceae et réunit plus de 280 espèces, ainsi que de nombreux hybrides et variétés sélectionnés depuis le 18e siècle. Ces accessions regroupent notamment de pélargoniums commercialisés en tant que plantes ornementales (comme les P. x hortorum) mais également des pélargoniums odorants (comme les P. x hybridum cv rosat) qui sont cultivés pour leur huile essentielle (HE). L’HE de P. rosat est stockée dans des structures glandulaires (trichomes glandulaires) présentes sur les feuilles et se compose principalement de mono- et sesqui-terpénoïdes.

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18

Rawlinson, Catherine. "Characterising the specialised metabolism of the wheat Pyrenophora tritici-repentis interaction." Thesis, Curtin University, 2020. http://hdl.handle.net/20.500.11937/82846.

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The necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr) causes the economically damaging tan spot disease of wheat. The project investigates the specialised metabolites (SMs) utilised by the pathogen for interaction with the host. This thesis describes the first characterised biosynthetic gene cluster in Ptr, the first report of an amide containing phthalide and the first structurally characterised small molecule necrotrophic effector. Characterisation of host-pathogen holds significant potential in developing control strategies for tan spot disease.

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19

Coq--Etchegaray, Domitille. "Déterminisme génétique de la variation des métabolites spécialisés en lien avec les interactions biotiques chez une plante de grande culture et une espèce forestière dominante." Electronic Thesis or Diss., Bordeaux, 2023. http://www.theses.fr/2023BORD0478.

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Un des mécanismes importants dans la protection et la défense des plantes face aux contraintes de l’environnement est la production de composés chimiques appelés métabolites “spécialisés”. Au sein des espèces, on observe une grande variabilité de production des métabolites spécialisés entre les individus. Cette variation peut être une réponse des individus à des stress biotiques ou abiotiques, mais elle peut aussi avoir pour origine des différences génétiques entre individus. En effet, la variation naturelle de la production de métabolites spécialisés, qu’elle soit constitutive ou induite en r

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20

Mütze, Ulrike, Alena Gerlinde Thiele, Christoph Baerwald, Uta Ceglarek, Wieland Kiess, and Skadi Beblo. "Ten years of specialized adult care for phenylketonuria." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205208.

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Background: Specialized adult care of phenylketonuria (PKU) patients is of increasing importance. Adult outpatient clinics for inherited errors of metabolism can help to achieve this task, but experience is limited. Ten years after establishment of a coordinated transition process and specialised adult care for inherited metabolic diseases, adult PKU care was evaluated with respect to metabolic control, therapy satisfaction, life satisfaction, sociodemographic data, economical welfare as well as pregnancy outcome. Methods: All PKU patients transferred from paediatric to adult care between 2005

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21

Choufa, Caroline. "Transferts d’éléments conjugatifs et mobilisation d’ADN chromosomique dans une population de Streptomyces." Electronic Thesis or Diss., Université de Lorraine, 2023. http://www.theses.fr/2023LORR0395.

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Les Streptomyces sont des bactéries ubiquistes des sols où elles interagissent avec les organismes environnants via un large répertoire de métabolites spécialisés. En étudiant une population rhizosphérique de Streptomyces (des souches de même espèce et isolées du même micro-habitat rhizosphérique), il a été montré que les flux de gènes massifs promeuvent une évolution rapide des individus, permettant leur diversification génétique et fonctionnelle dans le sol forestier. L'objectif de la recherche est de déterminer l'ampleur de ces flux de gènes au sein de la population et les mécanismes respon

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22

Barthélémy, Morgane. "Etude de la diversité chimique et biologique d’endophytes de palmiers." Thesis, Sorbonne université, 2019. http://www.theses.fr/2019SORUS563.

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Dans cette étude, le palmier Astrocaryum sciophilum a été choisi comme modèle pour l'étude de ses endophytes foliaires. Du fait de sa longévité, nous avons cherché à mettre en évidence une communauté compétitive d’endophytes en fonction de l’âge de ses feuilles. Afin d’évaluer si les métabolites produits par ces endophytes pourraient être utilisés en santé humaine, les extraits de chaque endophyte ont été testés contre Staphylococcus aureus résistant à la méticilline (SARM) ainsi que pour leur activité quorum quenching (QQ). En parallèle, afin d’identifier un rôle écologique de protection de l

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23

Pires, Vanessa Leal 1988. "Impact of global warming on the spawning success, metabolism and embryogenesis of a specialized soft coral-feeding nudibranch, Armina maculata." Master's thesis, 2012. http://hdl.handle.net/10451/7705.

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Tese de mestrado. Biologia (Ecologia Marinha). Universidade de Lisboa, Faculdade de Ciências, 2012<br>Temperature is considered as one of the most important physical variables controlling the life of marine organisms. Thus, understanding how they will respond to future thermal scenarios is of great relevance. The present study aimed to investigate, for the first time, the spawning success, embryonic development and metabolic rates of a specialized soft coral-feeding nudibranch, Armina maculata, under different thermal scenarios, which reflect: i) the present-day average spring (18ºC), early-su

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24

Jirásková, Petra. "Biosyntéza propylprolinové stavební jednotky linkomycinu." Doctoral thesis, 2020. http://www.nusl.cz/ntk/nusl-437111.

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The clinically used antibiotic lincomycin consists of an amino-sugar and an amino-acid moiety. The incorporated amino-acid 4-propyl-L-prolin (PPL) is very important for the linomycin bioactivity, as evidenced by the lower activity of the related antibiotic celesticetin, which incorporates proteinogenic L-prolin instead. Gene clusters for the biosynthesis of both lincosamides are published and reflect a common basis - biosynthesis of amino-sugar precursor and condensation reactions. Additionally, in the biosynthetic gene cluster for lincomycin there is a sub-cluster of genes encoding the biosyn

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25

Negri, Stefano. "The tomato serotonin pathway: unravelling the puzzling biological roles of plant indolamines." Doctoral thesis, 2020. http://hdl.handle.net/11562/1017074.

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Tryptamine and serotonin are specialized metabolites belonging to the group of tryptophan-derived indolamines that have been demonstrated to be widespread among all the living kingdoms, in which evolution shaped very different distributions and functional versatility. First discovered in humans, these metabolites were later detected in plants in which, despite their wide occurrence in several plant families, the study of their biological roles has been largely neglected. Tryptamine, due to its central position as a precursor of many plant specialized metabolites, including serotonin, has long

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26

Steiningerová, Lucie. "Úloha F420H2-závislých reduktas v biosyntéze bioaktivních mikrobiálních metabolitů inkorporujících 4-alkyl-L-prolinový derivát." Doctoral thesis, 2020. http://www.nusl.cz/ntk/nusl-437108.

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Antitumor pyrrolobenzodiazepines (PBDs), lincosamide antibiotics, quorum sensing molecule hormaomycin, and antituberculotic griselimycin are structurally and functionally diverse groups of actinobacterial metabolites. The common feature of these compounds is the incorporation of L-tyrosine- or L-leucine-derived 4-alkyl-L-proline derivatives (APDs) in their structures. APD biosynthesis involves a set of up to six homologous proteins. According to their proposed order in the biosynthesis of 4-propyl-L-proline, a model APD of lincosamide lincomycin, the homologous proteins were named Apd1 - Apd6.

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