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Journal articles on the topic 'Spectrofluorometry'

Author: Grafiati
Published: 4 June 2025
Last updated: 23 June 2025

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1

Yilmaz, Bilal, Kadem Meral, Ali Asci, and Yavuz Organer. "Determination of metoprolol in pure and pharmaceutical dosage forms by spectrofluorometry and high performance liquid chromatography." Chemical Industry and Chemical Engineering Quarterly 17, no. 1 (2011): 25–31. http://dx.doi.org/10.2298/ciceq100422047y.

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In this study, a new and rapid spectrofluorometry and high performance liquid chromatography (HPLC) methods were developed for determination of metoprolol in pure and pharmaceutical dosage forms. The solvent system, wavelength of detection and chromatographic conditions were optimized in order to maximize the sensitivity of both the proposed methods. The linearity was established over the concentration range of 50-4000 ng ml-1 for spectrofluorometry and 5.0-300 ng ml-1 for HPLC methods. The intra- and inter-day relative standard deviation (RSD) was less than 4.14 and 3.86% for spectrofluorometry and HPLC, respectively. Limit of quantitation was determined as 30 and 5.0 ng ml-1 for spectrofluorometry and HPLC, respectively. No interference was found from tablet excipients at the selected assay conditions. The methods were applied for the quality control of commercial metoprolol dosage forms to quantify the drug and to check the formulation content uniformity.
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2

Yilmaz, Bilal, Yucel Kadioglu, Kadem Meral, and Yavuz Onganer. "Determination of human growth hormone in pure and pharmaceutical dosage form by spectrofluorometry and high performance liquid chromatography." Chemical Industry and Chemical Engineering Quarterly 18, no. 3 (2012): 399–405. http://dx.doi.org/10.2298/ciceq111118015y.

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In this study, a new and rapid spectrofluorometry and high performance liquid chromatography (HPLC) methods were developed for determination of human growth hormone in pure and pharmaceutical dosage form. The solvent system, wavelength of detection and chromatographic conditions were optimized in order to maximize the sensitivity of both the proposed methods. The linearity was established over the concentration range of 1.25-50?g mL-1 for spectrofluorometry and 10-75?g mL-1 for HPLC method. The intra- and inter-day relative standard deviation (RSD) was less than 8.46 and 5.98% for spectrofluorometry and HPLC, respectively. Limits of quantitation were determined as 0.075 and 7.5?g mL-1 for spectrofluorometry and HPLC, respectively. No interference was found from tablet excipients at the selected assay conditions. The methods were applied for the quality control of commercial growth hormone dosage form to quantify the drug and to check the formulation content uniformity.
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3

Oyelakin, Oladele, Moumouny Traoré, El Hadji Babacar Mbye, et al. "Spectrofluorometric Determination of Putrescine: Optimization of the Putrescine–Orthophthaldehyde Complex Using Spectrofluorometry." Journal of AOAC INTERNATIONAL 99, no. 6 (2016): 1642–44. http://dx.doi.org/10.5740/jaoacint.16-0136.

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Abstract In alkaline medium, the complex formed between putrescine and orthophthalaldehyde was studied using spectrofluorescence. The derivative is kinetically stable 24 h after complexation. The stoichiometry of the complex is 1:1 at maximum fluorescence intensity, also 24 h after complexation.
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4

Sun, Zheng Juan, Jun Ze Jiang, and Yuan Fang Li. "A sensitive and selective sensor for biothiols based on the turn-on fluorescence of the Fe-MIL-88 metal–organic frameworks–hydrogen peroxide system." Analyst 140, no. 24 (2015): 8201–8. http://dx.doi.org/10.1039/c5an01673h.

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5

KAIGA, Nobuyoshi, Souichiro NAKANO, Yoshihiko TEZUKA, and Tadahiro ISHII. "Evaluation of Tap Water Quality by Spectrofluorometry." Journal of Japan Society on Water Environment 22, no. 1 (1999): 54–60. http://dx.doi.org/10.2965/jswe.22.54.

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6

Crespi, Francesco, Anna C. Croce, Sabrina Fiorani, Barbara Masala, Christian Heidbreder, and Giovanni Bottiroli. "In vivo autofluorescence spectrofluorometry of central serotonin." Journal of Neuroscience Methods 140, no. 1-2 (2004): 67–73. http://dx.doi.org/10.1016/j.jneumeth.2004.06.019.

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7

Janhom, Tansiphorn, Suraphong Wattanachira, and Prasert Pavasant. "Characterization of brewery wastewater with spectrofluorometry analysis." Journal of Environmental Management 90, no. 2 (2009): 1184–90. http://dx.doi.org/10.1016/j.jenvman.2008.05.008.

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8

Voss, Edward W. "Kinetic measurements of molecular interactions by spectrofluorometry." Journal of Molecular Recognition 6, no. 2 (1993): 51–58. http://dx.doi.org/10.1002/jmr.300060203.

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9

Levin, Robert M., Michael R. Ruggieri, Joseph A. Hypolite, S. Bruce Malkowicz, and Alan J. Wein. "Surface spectrofluorometry of the rabbit urinary bladder." Neurourology and Urodynamics 6, no. 2 (1987): 109–18. http://dx.doi.org/10.1002/nau.1930060208.

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10

Fuller, Mark E., Brian J. Mailloux, Sheryl H. Streger, et al. "Application of a Vital Fluorescent Staining Method for Simultaneous, Near-Real-Time Concentration Monitoring of Two Bacterial Strains in an Atlantic Coastal Plain Aquifer in Oyster, Virginia." Applied and Environmental Microbiology 70, no. 3 (2004): 1680–87. http://dx.doi.org/10.1128/aem.70.3.1680-1687.2004.

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ABSTRACT Two differentially labeled bacterial strains were monitored in near-real time during two field-scale bacterial transport experiments in a shallow aquifer in July 2000 and July 2001. Comamonas sp. strain DA001 and Acidovorax sp. strain OY-107 were grown and labeled with the vital fluorescent stain TAMRA/SE (5 [and -6]-carboxytetramethylrhodamine, succinimidyl ester) or CFDA/SE (5 [and -6]-carboxyfluorescein diacetate, succinimidyl ester). Fluorescently labeled cells and a conservative bromide tracer were introduced into a suboxic superficial aquifer, followed by groundwater collection from down-gradient multilevel samplers. Cells were enumerated in the field by microplate spectrofluorometry, with confirmatory analyses for selected samples done in the laboratory by epifluorescence microscopy, flow cytometry, and ferrographic capture. There was general agreement in the results from all of the vital-stain-based enumeration methods, with differences ranging from <10% up to 40% for the analysis of identical samples between different tracking methods. Field analysis by microplate spectrofluorometry was robust and efficient, allowing thousands of samples to be analyzed in quadruplicate for both of the injected strains. The near-real-time data acquisition allowed adjustments to the predetermined sampling schedule to be made. The microplate spectrofluorometry data sets for the July 2000 and July 2001 experiments allowed the transport of the injected cells to be related to the site hydrogeology and injection conditions and enabled the assessment of differences in the transport of the two strains. This near-real-time method should prove effective for a number of microbial ecology applications.
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11

Yatsenko, T. A. "POLYCLONAL ANTIBODIES AGAINST HUMAN PLASMINOGEN: PURIFICATION, CHARACTERIZATION AND APPLICATION." Biotechnologia Acta 13, no. 6 (2020): 50–57. http://dx.doi.org/10.15407/biotech13.06.050.

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The plasminogen/plasmin system plays a crucial role in fibrinolysis and regulation of cell functions in a wide range of normal and pathological processes. Investigation of plasminogen/plasmin functions requires the availability of well-characterized and effective molecular tools, such as antibodies. In the present work, the isolation and characterization of rabbit polyclonal antibodies against human plasminogen are described and approaches for the identification of plasminogen and its fragments using the purified antibodies are demonstrated. For the antibodies isolation, standard animal immunization and blood collection procedures, serum isolation, protein salting out and affinity chromatography were performed. For the antibodies characterization and application, the following methods were used: enzyme linked immunoassay (ELISA), Western blotting, FITC-protein conjugation, flow cytometry and spectrofluorometry. The obtained polyclonal rabbit anti-human plasminogen antibodies interacted with human Glu- and Lys-plasminogen, kringles 1-3 and 1-4 of plasminogen, mini-plasminogen, the heavy and light chain of plasmin. We propose the application of anti-plasminogen antibodies for the direct ELISA, Western blot analysis, and for flow cytometry and spectrofluorometric analysis of plasminogen binding with cells. The obtained anti-plasminogen antibodies are promising tools for the investigation of plasminogen/plasmin system functions, either fibrinolytic or signaling.
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12

Sutaykina, A. S., N. A. Krishtanova, E. V. Vishnyakov, and T. D. Sineva. "Assessment of the Quality of a Compounded Multivitamin Syrup for Children: Development of Analytical Procedures." Regulatory Research and Medicine Evaluation 14, no. 4 (2024): 411–18. http://dx.doi.org/10.30895/1991-2919-2024-649.

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INTRODUCTION. The range of liquid multivitamins for toddlers approved in the Russian Federation comprises two imported syrups. Pharmacy compounding of vitamin syrups will meet the demand for optimally formulated multivitamins in the pharmaceutical market. Therefore, a necessary step for their release into medical practice is to develop and validate analytical procedures for assessing the quality of such medicinal products.AIM. This study aimed to select and adapt analytical procedures for assessing the quality of a compounded multivitamin product for children.MATERIALS AND METHODS. The study focused on a compounded sorbitol syrup containing vitamins C, PP, B1, B2, and B6. The quantities of vitamin components in the syrup corresponded to the recommended daily intake for children aged 1–3 years. For identification, the authors used qualitative reactions and spectrophotometry. Indicator-free direct iodometry was selected for the quantitative determination of ascorbic acid. Alkalimetry with bromothymol blue was used to quantitate nicotinic acid in the presence of ascorbic acid, pyridoxine hydrochloride, and thiamine hydrochloride. The sum of thiamine hydrochloride and pyridoxine hydrochloride was determined by direct argentometry (Fajans method) with bromophenol blue in an acetic acid medium. Riboflavin quantification involved visible spectrophotometry at 445 nm and spectrofluorometry. The sorbitol content was measured by refractometry, and the calculations accounted for the content of other syrup components. The validation was conducted in line with the applicable pharmacopoeial requirements (State Pharmacopoeia of the Russian Federation).RESULTS. The authors selected analytical procedures for the quality evaluation of the multivitamin syrup containing five water-soluble vitamins (С, РР, В1, В2, В6) and sorbitol. These analytical procedures were adapted to the simultaneous presence of riboflavin, ascorbic acid, nicotinic acid, thiamine hydrochloride, and pyridoxine hydrochloride. The authors developed analytical procedures for the determination of riboflavin by spectrofluorometry and spectrophotometry and for the selective determination of thiamine hydrochloride by photometry after a precipitation reaction with Reinecke’s salt. The analytical procedure for the determination of riboflavin by spectrofluorometry was validated for specificity, accuracy, linearity, precision (repeatability and intermediate precision), and range.CONCLUSIONS. The study confirmed the applicability of the analytical procedures that were selected and adapted for all components of the multivitamin syrup. The authors identified the conditions for the selective determination of thiamine hydrochloride by spectrophotometry after a precipitation reaction with Reinecke’s salt. The analytical procedure for the quantitative determination of riboflavin by spectrofluorometry was validated and considered suitable for use in analytical laboratories for the quantitative determination of riboflavin in multivitamin mixtures and for syrup stability testing.
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13

Crespi, Francesco, Anna C. Croce, Sabrina Fiorani, Barbara Masala, Christian Heidbreder, and Giovanni Bottiroli. "Autofluorescence Spectrofluorometry of central nervous system (CNS) neuromediators." Lasers in Surgery and Medicine 34, no. 1 (2004): 39–47. http://dx.doi.org/10.1002/lsm.10240.

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14

Suvarapu, Lakshmi Narayana, and Sung-Ok Baek. "Recent Developments in the Speciation and Determination of Mercury Using Various Analytical Techniques." Journal of Analytical Methods in Chemistry 2015 (2015): 1–18. http://dx.doi.org/10.1155/2015/372459.

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This paper reviews the speciation and determination of mercury by various analytical techniques such as atomic absorption spectrometry, voltammetry, inductively coupled plasma techniques, spectrophotometry, spectrofluorometry, high performance liquid chromatography, and gas chromatography. Approximately 126 research papers on the speciation and determination of mercury by various analytical techniques published in international journals since 2013 are reviewed.
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15

Croce, Anna C., and Francesca Scolari. "The Bright Side of the Tiger: Autofluorescence Patterns in Aedes albopictus (Diptera, Culicidae) Male and Female Mosquitoes." Molecules 27, no. 3 (2022): 713. http://dx.doi.org/10.3390/molecules27030713.

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Light-based events in insects deserve increasing attention for various reasons. Besides their roles in inter- and intra-specific visual communication, with biological, ecological and taxonomical implications, optical properties are also promising tools for the monitoring of insect pests and disease vectors. Among these is the Asian tiger mosquito, Aedes albopictus, a global arbovirus vector. Here we have focused on the autofluorescence characterization of Ae. albopictus adults using a combined imaging and spectrofluorometric approach. Imaging has evidenced that autofluorescence rises from specific body compartments, such as the head appendages, and the abdominal and leg scales. Spectrofluorometry has demonstrated that emission consists of a main band in the 410–600 nm region. The changes in the maximum peak position, between 430 nm and 500 nm, and in the spectral width, dependent on the target structure, indicate the presence, at variable degrees, of different fluorophores, likely resilin, chitin and melanins. The aim of this work has been to provide initial evidence on the so far largely unexplored autofluorescence of Ae. albopictus, to furnish new perspectives for the set-up of species- and sex-specific investigation of biological functions as well as of strategies for in-flight direct detection and surveillance of mosquito vectors.
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16

Ioannides, Ioannis M., Vinay P. Shedbalkar, and Constantin A. Rebeiz. "Quantitative Determination of 2-Monovinyl Protochlorophyll(ide)bby Spectrofluorometry." Analytical Biochemistry 249, no. 2 (1997): 241–44. http://dx.doi.org/10.1006/abio.1997.2179.

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17

Simonazzi, Mara, Laura Pezzolesi, Franca Guerrini, et al. "Improvement of In Vivo Fluorescence Tools for Fast Monitoring of Freshwater Phytoplankton and Potentially Harmful Cyanobacteria." International Journal of Environmental Research and Public Health 19, no. 21 (2022): 14075. http://dx.doi.org/10.3390/ijerph192114075.

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The use of multi-wavelength spectrofluorometers for the fast detection of algal taxa, based on chlorophyll a (Chl-a) emission spectra, has become a common practice in freshwater water management, although concerns about their accuracy have been raised. Here, inter-laboratory comparisons using monoalgal cultures have been performed to assess the reliability of different spectrofluorometer models, alongside Chl-a extraction methods. Higher Chl-a concentrations were obtained when using the spectrofluorometers than extraction methods, likely due to the poor extraction efficiencies of solvents, highlighting that traditional extraction methods could underestimate algal or cyanobacterial biomass. Spectrofluorometers correctly assigned species to the respective taxonomic group, with low and constant percent attribution errors (Chlorophyta and Euglenophyceae 6–8%, Cyanobacteria 0–3%, and Bacillariophyta 10–16%), suggesting that functioning limitations can be overcome by spectrofluorometer re-calibration with fresh cultures. The monitoring of a natural phytoplankton assemblage dominated by Chlorophyta and Cyanobacteria gave consistent results among spectrofluorometers and with microscopic observations, especially when cell biovolume rather than cell density was considered. In conclusion, multi-wavelength spectrofluorometers were confirmed as valid tools for freshwater monitoring, whereas a major focus on intercalibration procedures is encouraged to improve their reliability and broaden their use as fast monitoring tools to prevent environmental and public health issues related to the presence of harmful cyanobacteria.
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18

James, Brian W., W. Stuart Mauchline, P. Julian Dennis, C. William Keevil, and Robin Wait. "Poly-3-Hydroxybutyrate in Legionella pneumophila, an Energy Source for Survival in Low-Nutrient Environments." Applied and Environmental Microbiology 65, no. 2 (1999): 822–27. http://dx.doi.org/10.1128/aem.65.2.822-827.1999.

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ABSTRACT Chloroform-soluble material was extracted from two strains ofL. pneumophila serogroup 1 following growth in continuous culture. The purified material was identified as poly-3-hydroxybutyrate (PHB) by nuclear magnetic resonance spectroscopy and by gas chromatography-mass spectrometry. PHB yields of up to 16% of cell dry weight were extracted from culture samples. The PHB was located in electron-dense intracellular inclusions, which fluoresced bright yellow when stained with the lipophilic dye Nile red. A Nile red spectrofluorometric assay provided a more accurate and reliable determination of the PHB content. PHB accumulation increased threefold during iron-limited culture and was inversely related to the concentration of iron metabolized. Chemostat-grown cells survived in a culturable state for at least 600 days when incubated at 24°C in a low-nutrient tap water environment. Nile red spectrofluorometry and flow cytometry demonstrated that PHB reserves were utilized during starvation. PHB utilization, as revealed by the decline in mean cellular fluorescence and cell complexity, correlated with loss of culturability. Fluorescence microscopy provided visual evidence of PHB utilization, with a marked reduction in the number of Nile red-stained granules during starvation. Heat shock treatment failed to resuscitate nonculturable cells. This study demonstrates that L. pneumophila accumulates significant intracellular reserves of PHB, which promote its long-term survival under conditions of starvation.
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19

Croce, Anna C., Andrea Ferrigno, Clarissa Berardo, Giovanni Bottiroli, Mariapia Vairetti, and Laura G. Di Pasqua. "Spectrofluorometric Analysis of Autofluorescing Components of Crude Serum from a Rat Liver Model of Ischemia and Reperfusion." Molecules 25, no. 6 (2020): 1327. http://dx.doi.org/10.3390/molecules25061327.

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Autofluorescence (AF) of crude serum was investigated with reference to the potential of its intrinsic AF biomarkers for the noninvasive diagnosis of liver injury. Spectral parameters of pure compounds representing retinol (vitamin A) and fluorescing free fatty acids were characterized by spectrofluorometry, to assess spectral parameters for the subsequent AF analysis of serum, collected from rats undergoing liver ischemia/reperfusion (I/R). Differences in AF spectral profiles detected between control and I/R were due to the increase in the AF components representing fatty acids in I/R serum samples. No significant changes occurred for retinol levels, consistently with the literature reporting that constant retinol levels are commonly observed in the blood, except for malnutrition or chronic severe liver disease. Conversely, fatty acids, in particular arachidonic and linoleic acid and their derivatives, act as modulating agents in inflammation, representing both a protective and damaging response to stress stimuli. The biometabolic and pathophysiological meaning of serum components and the possibility of their direct detection by AF spectrofluorometry open up interesting perspectives for the development of AF serum analysis, as a direct, cost effective, supportive tool to assess liver injury and related systemic metabolic alterations, for applications in experimental biomedicine and foreseen translation to the clinics.
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20

Capitan, Fermin, Enrique Alonso, Ramiro Avidad, L. F. Capitan-Vallvey, and Jose Luis Vilchez. "Determination of thiabendazole residues in waters by solid-phase spectrofluorometry." Analytical Chemistry 65, no. 10 (1993): 1336–39. http://dx.doi.org/10.1021/ac00058a007.

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21

Adlina, Salsabila, Slamet Ibrahims, and Benny Permana. "Development of Metformin and Glimepiride Analysis Methods Using TLC-Spectrofluorometry." Indonesian Journal of Pharmaceutical Science and Technology 10, no. 3 (2023): 164. http://dx.doi.org/10.24198/ijpst.v10i3.33708.

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22

Sharma, Hemraj, Hari Prasad Sapkota, and Nim Bahadur Dangi. "A Brief Review of Analytical Methods for the Estimation of Allopurinol in Pharmaceutical Formulation and Biological Matrices." International Journal of Analytical Chemistry 2021 (June 5, 2021): 1–12. http://dx.doi.org/10.1155/2021/5558651.

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This review article represents the collection and discussion of various analytical methods available in the literature for the determination of allopurinol (ALLP) in pharmaceutical and biological samples consisting of HPLC, UV-visible method, near-IR spectroscopy, spectrofluorometry, capillary electrophoresis, polarography, voltammetry, and hyphenated techniques such as LC-MS, LC-MS/MS, UPLC-MS/MS, and GC-MS. The anticipated review provides details about the comparative utilization of various analytical techniques for the determination of ALLP. The present review article can be effectively explored to conduct future analytical investigation for the estimation of ALLP.
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23

LAGE-YUSTY, María Asunción, Javier LÓPEZ-GONZÁLEZ, and Jesús SIMAL-LOZANO. "Resolution of 13 Polycyclic Aromatic Hydrocarbons by Constant-wavelength Synchronous Spectrofluorometry." Analytical Sciences 21, no. 10 (2005): 1203–6. http://dx.doi.org/10.2116/analsci.21.1203.

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24

Fidler, Vlastimil, Stefan Vajda, Zuzana Limpouchová, Jiří Dvořák, Karel Procházka, and Bohumil Bednář. "Study of polymer chain dynamics in solution by time-resolved spectrofluorometry." Collection of Czechoslovak Chemical Communications 54, no. 11 (1989): 3011–24. http://dx.doi.org/10.1135/cccc19893011.

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The methodology of polarization time-resolved fluorometry and interpretation of its results are outlined at a general level, and the measurement on and use of facilities of the Edinburgh Instruments Model 299T apparatus are discussed in detail. The dynamics of conformational changes in chains of poly(methacrylic acid) containing covalently bonded dansyl labels are studied in aqueous solutions at various pH. It is shown that at pH > 6, the shorter effective rational correlation time τr < 2 ns corresponds to the rotation of the free dansyl label about bonds by which it is attached to the polymeric chain; at pH < 4 the longer effective rational correlation time τr = 20-26 ns corresponds to the rotation of the compact spherical formation constituted by a part of the collapsed polymeric chain in which the label is fixed and whose relative molecular mass is approx. 15 000-20 000.
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25

Armstrong, Donald, Jiri Wilhelm, Frantisek Smid, and Milan Elleder. "Chromatography and spectrofluorometry of brain fluorophores in neuronal ceroid lipofuscinosis (NCL)." Mechanisms of Ageing and Development 64, no. 3 (1992): 293–302. http://dx.doi.org/10.1016/0047-6374(92)90085-r.

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26

Paturel, Laurent, Abdel-Ilah Saber, Claude Fachinger, Joel Suptil, and Christophe Turnar. "A Data Acquisition and Processing Software for High-Resolution Shpol'Skii Spectrofluorometry." Polycyclic Aromatic Compounds 13, no. 3 (1999): 151–64. http://dx.doi.org/10.1080/10406639908020560.

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27

Daxhelet, G. A., M. M. Coene, P. P. Hoet, and C. G. Cocito. "Spectrofluorometry of dyes with DNAs of different base composition and conformation." Analytical Biochemistry 179, no. 2 (1989): 401–3. http://dx.doi.org/10.1016/0003-2697(89)90152-8.

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Rusinova, Radda, Dorothy Kim, Crina Nimigean, and Olaf S. Andersen. "A New Assay for Ion Channel Function using Stopped Flow Spectrofluorometry." Biophysical Journal 104, no. 2 (2013): 373a. http://dx.doi.org/10.1016/j.bpj.2012.11.2074.

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Farzami, B., A. A. Moosavi-Movahedi, and G. A. Naderi. "Elucidation of values for Ca2+ binding sites in calmodulin by spectrofluorometry." International Journal of Biological Macromolecules 16, no. 4 (1994): 181–86. http://dx.doi.org/10.1016/0141-8130(94)90049-3.

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Ratnakumari, Lingamaneni, and Hugh C. Hemmings. "Inhibition of Presynaptic Sodium Channels by Halothane." Anesthesiology 88, no. 4 (1998): 1043–54. http://dx.doi.org/10.1097/00000542-199804000-00025.

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Background Recent electrophysiologic studies indicate that clinical concentrations of volatile general anesthetic agents inhibit central nervous system sodium (Na+) channels. In this study, the biochemical effects of halothane on Na+ channel function were determined using rat brain synaptosomes (pinched-off nerve terminals) to assess the role of presynaptic Na+ channels in anesthetic effects. Methods Synaptosomes from adult rat cerebral cortex were used to determine the effects of halothane on veratridine-evoked Na+ channel-dependent Na+ influx (using 22Na+), changes in intrasynaptosomal [Na+] (using ion-specific spectrofluorometry), and neurotoxin interactions with specific receptor sites of the Na+ channel (by radioligand binding). The potential physiologic and functional significance of these effects was determined by measuring the effects of halothane on veratridine-evoked Na+ channel-dependent glutamate release (using enzyme-coupled spectrofluorometry). Results Halothane inhibited veratridine-evoked 22Na+ influx (IC50 = 1.1 mM) and changes in intrasynaptosomal [Na+] (concentration for 50% inhibition [IC50] = 0.97 mM), and it specifically antagonized [3H]batrachotoxinin-A 20-alpha-benzoate binding to receptor site two of the Na+ channel (IC50 = 0.53 mM). Scatchard and kinetic analysis revealed an allosteric competitive mechanism for inhibition of toxin binding. Halothane inhibited veratridine-evoked glutamate release from synaptosomes with comparable potency (IC50 = 0.67 mM). Conclusions Halothane significantly inhibited Na+ channel-mediated Na influx, increases in intrasynaptosomal [Na+] and glutamate release, and competed with neurotoxin binding to site two of the Na+ channel in synaptosomes at concentrations within its clinical range (minimum alveolar concentration, 1-2). These findings support a role for presynaptic Na+ channels as a molecular target for general anesthetic effects.
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Huang, Shuangbing, Yanxin Wang, Ling Cao, Kunfu Pi, Mei Yu, and Emilie Even. "Multidimensional spectrofluorometry characterization of dissolved organic matter in arsenic-contaminated shallow groundwater." Journal of Environmental Science and Health, Part A 47, no. 10 (2012): 1446–54. http://dx.doi.org/10.1080/10934529.2012.672390.

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32

Sestak, Z. "van Kooten, O., Snel, J.F.H. (ed.): Plant Spectrofluorometry: Applications and Basic Research." Photosynthetica 40, no. 4 (2002): 528. http://dx.doi.org/10.1023/a:1024345212264.

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KAWAKUBO, Susumu, Takashi NETSU, and Masaaki IWATSUKI. "Speciation of Vanadium in Rain Water by Size-Fractionation and Catalytic Spectrofluorometry." Analytical Sciences 13, no. 6 (1997): 1033–35. http://dx.doi.org/10.2116/analsci.13.1033.

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34

Mortensen, A., E. Bjern Jensen, P. Bernth Petersen, S. Husted, and F. Andreasen. "The Determination of Naproxen by Spectrofluorometry and its Binding to Serum Proteins." Acta Pharmacologica et Toxicologica 44, no. 4 (2009): 277–83. http://dx.doi.org/10.1111/j.1600-0773.1979.tb02330.x.

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35

Kusunoki, Yoko, Fumio Imamura, Hiroshi Uda, Masayuki Mano, and Takeshi Horai. "Early Detection of Lung Cancer With Laser-Induced Fluorescence Endoscopy and Spectrofluorometry." Chest 118, no. 6 (2000): 1776–82. http://dx.doi.org/10.1378/chest.118.6.1776.

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36

Peacock, Melissa Blakely, and Raphael M. Kudela. "A method for determining alkaline phosphatase activity in marine phytoplankton using spectrofluorometry." Journal of Microbiological Methods 89, no. 3 (2012): 209–12. http://dx.doi.org/10.1016/j.mimet.2012.03.007.

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37

Paturel, Laurent, Abdel-Ilah Saber, Evelyne Combet, and Robert Joumard. "Analysis of PAH Emissions from Passenger Cars by High Resolution Shpol'skii Spectrofluorometry." Polycyclic Aromatic Compounds 9, no. 1-4 (1996): 331–39. http://dx.doi.org/10.1080/10406639608031235.

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38

Hussain, Husniza, Santhana Raj L., Syahida Ahmad, et al. "Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay." Cogent Food & Agriculture 5, no. 1 (2019): 1582398. http://dx.doi.org/10.1080/23311932.2019.1582398.

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39

Blanco, C. Cruces, and F. García Sánchez. "Determination of Gibberellic Acid Residues on Fruits by Synchronous Scanning Derivative Spectrofluorometry." Journal of AOAC INTERNATIONAL 69, no. 1 (1986): 105–9. http://dx.doi.org/10.1093/jaoac/69.1.105.

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Abstract A synchronous derivative spectrofluorometric method is described for the determination of the plant growth regulator, gibberellic acid (GA3). The method is based on the formation of a fluorogen in concentrated sulfuric acid. The reaction is carried out at 85% sulfuric acid and in aqueous medium. The common fluorometric method with a linear dynamic range of 137-400 ppb, and a detection limit of 48 ppb is described. The synchronous first and second derivative method has linear dynamic ranges between 7.6-40 ppb and 12-40 ppb, with detection limits of 3.5 and 6.7 ppb, respectively. The influence of reaction variables and of other plant growth regulators present, and the application to residues on oranges, lemons, and grapes, are also described.
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40

Güler, Nehir, Mervegül Maden, Sezgin Bakırdere, O. Yavuz Ataman, and Mürvet Volkan. "Speciation of selenium in vitamin tablets using spectrofluorometry following cloud point extraction." Food Chemistry 129, no. 4 (2011): 1793–99. http://dx.doi.org/10.1016/j.foodchem.2011.05.007.

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41

Hagedorn, Andrea, Raymond L. Legge, and Hector Budman. "Evaluation of spectrofluorometry as a tool for estimation in fed-batch fermentations." Biotechnology and Bioengineering 83, no. 1 (2003): 104–11. http://dx.doi.org/10.1002/bit.10649.

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42

Luchtefeld, Ronald G. "Multiresidue Method for Determining Substituted Urea Herbicides in Foods by Liquid Chromatography." Journal of AOAC INTERNATIONAL 70, no. 4 (1987): 740–45. http://dx.doi.org/10.1093/jaoac/70.2.740.

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Abstract A method is described for determining substituted urea herbicides in foods. The residues are extracted from the product with methanol, and the food coextractives are removed by using solvent partitioning and Florisil column chromatography. The extract is analyzed using liquid chromatography with postcolumn photodegradation, chemical derivatization with orthophthalaldehyde, and spectrofluorometry. Recoveries were determined by spiking 8 different food products with 6 phenylureas—chlorbromuron, chloroxuron, diuron, fluometuron, linuron, and metobromuron—at 0.05 and 0.5 ppm. Three determinations were made at each level for each product. Average recovery at 0.05 ppm was 95% (with a standard deviation of 7.9%), and at 0.5 ppm, 98% (with a standard deviation of 6.9%).
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43

Luchtefeld, Ronald G. "Multiresidue Method for Determining Substituted Urea Herbicides in Foods by Liquid Chromatography." Journal of AOAC INTERNATIONAL 70, no. 4 (1987): 740–45. http://dx.doi.org/10.1093/jaoac/70.4.740.

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Abstract A method is described for determining substituted urea herbicides in foods. The residues are extracted from the product with methanol, and the food coextractives are removed by using solvent partitioning and Florisil column chromatography. The extract is analyzed using liquid chromatography with postcolumn photodegradation, chemical derivatization with orthophthalaldehyde, and spectrofluorometry. Recoveries were determined by spiking 8 different food products with 6 phenylureas—chlorbromuron, chloroxuron, diuron, fluometuron, linuron, and metobromuron—at 0.05 and 0.5 ppm. Three determinations were made at each level for each product. Average recovery at 0.05 ppm was 95% (with a standard deviation of 7.9%), and at 0.5 ppm, 98% (with a standard deviation of 6.9%).
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44

Rold�n, M., F. Thomas, S. Castel, A. Quesada, and M. Hern�ndez-Marin�. "Noninvasive Pigment Identification in Single Cells from Living Phototrophic Biofilms by Confocal Imaging Spectrofluorometry." Applied and Environmental Microbiology 70, no. 6 (2004): 3745–50. http://dx.doi.org/10.1128/aem.70.6.3745-3750.2004.

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ABSTRACT A new imaging technique for the analysis of fluorescent pigments from a single cell is reported. It is based on confocal scanning laser microscopy coupled with spectrofluorometric methods. The setup allows simultaneous establishment of the relationships among pigment analysis in vivo, morphology, and three-dimensional localization inside thick intact microbial assemblages.
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45

CAPITAN-VALLVEY, Luis Fermín, Enrique Jesús ALONSO, Ramiro AVIDAD, Monsalud del OLMO, and José Luis VILCHEZ. "Determination of Ultra-Traces of Anthracene in Water Samples by Solid-Phase Spectrofluorometry." Analytical Sciences 9, no. 1 (1993): 117–20. http://dx.doi.org/10.2116/analsci.9.117.

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46

Gao, Jing Jing, Ke Hua Xu, Ji Xi Hu, Hui Huang, and Bo Tang. "Determination of Trace Hydroxyl Radicals by Flow Injection Spectrofluorometry and Its Analytical Application." Journal of Agricultural and Food Chemistry 54, no. 21 (2006): 7968–72. http://dx.doi.org/10.1021/jf061452j.

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47

Berthoud, Thierry, Pierre Decambox, Barbara Kirsch, Patrick Mauchien, and Christophe Moulin. "Direct uranium trace analysis in plutonium solutions by time-resolved laser-induced spectrofluorometry." Analytical Chemistry 60, no. 13 (1988): 1296–99. http://dx.doi.org/10.1021/ac00164a011.

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48

Chen, R. F., D. B. Chadwick, and S. H. Lieberman. "The application of time-resolved spectrofluorometry to measuring benthic fluxes of organic compounds." Organic Geochemistry 26, no. 1-2 (1997): 67–77. http://dx.doi.org/10.1016/s0146-6380(96)00154-4.

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49

Picer, Mladen. "Simple spectrofluorometry methods for estimating petroleum hydrocarbons levels in various sea benthic organisms." Chemosphere 37, no. 4 (1998): 607–17. http://dx.doi.org/10.1016/s0045-6535(98)00086-1.

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50

Degelau, A., T. Scheper, J. E. Bailey, and C. Guske. "Fluorometric measurement of poly-? hydroxybutyrate in Alcaligenes eutrophus by flow cytometry and spectrofluorometry." Applied Microbiology and Biotechnology 42, no. 5 (1995): 653–57. http://dx.doi.org/10.1007/s002530050308.

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