Relevant bibliographies by topics / Spectroscopie de fluorescence

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Academic literature on the topic 'Spectroscopie de fluorescence'

Author: Grafiati
Published: 4 June 2021
Last updated: 11 January 2025

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Journal articles on the topic "Spectroscopie de fluorescence"

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ANDUEZA, D., B. P. MOUROT, A. AÏT-KADDOUR, S. PRACHE, and J. MOUROT. "Utilisation de la spectroscopie dans le proche infrarouge et de la spectroscopie de fluorescence pour estimer la la qualité et la traçabilité de la viande." INRA Productions Animales 28, no. 2 (January 13, 2020): 197–208. http://dx.doi.org/10.20870/productions-animales.2015.28.2.3025.

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Ces dernières années, la spectroscopie dans le proche infrarouge et la spectroscopie de fluorescence ont fait preuve d’une efficacité remarquable pour l’obtention d’estimations rapides et précises des paramètres de qualité des produits agricoles. Cette synthèse fait le point de l’état des connaissances sur l’application de ces technologies pour l’estimation de la qualité de la viande et des produits carnés. Un bref rappel des fondements théoriques des techniques est d’abord exposé. Les possibilités actuelles de leur application analytique aux différents paramètres de la qualité de la viande sont ensuite décrites. Les deux techniques montrent un grand potentiel pour la prédiction des composants du muscle et la détermination des différents paramètres de la qualité de la viande. Cependant, malgré l’important effort de recherche réalisé jusqu’à présent sur le sujet, la plupart des résultats montrent que leur utilisation pratique reste encore limitée. Les causes de ces performances sont décrites. Les résultats de l’utilisation de la spectroscopie dans le proche infrarouge dans une optique de traçabilité sont plus prometteurs. Cette technologie peut être utilisée pour détecter différentes types de viande entrant dans la composition des produits carnés, différencier la viande fraiche de la viande décongelée ou pour authentifier l’alimentation des animaux.
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McGuire, Hugo, and Rikard Blunck. "La spectroscopie de fluorescence pour détecter les changements de conformation en canaux unitaires." médecine/sciences 25, no. 11 (November 2009): 951–54. http://dx.doi.org/10.1051/medsci/20092511951.

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Ellingsen, G., and S. Fery-Forgues. "Application de la spectroscopie de fluorescence a l'étude du pétrole : le défi de la complexité." Revue de l'Institut Français du Pétrole 53, no. 2 (March 1998): 201–16. http://dx.doi.org/10.2516/ogst:1998019.

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Le Coupannec, F., and J. J. Peron. "Fractionnement et caractérisation des lixiviats de centres d'enfouissement technique de déchets ménagers: intérêt de la chromatographie liquide haute performance sur le gel d'exclusion stérique." Revue des sciences de l'eau 12, no. 3 (April 12, 2005): 529–43. http://dx.doi.org/10.7202/705364ar.

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L'ultrafiltration et la chromatographie d'exclusion stérique haute performance sont utilisées pour la séparation et la caractérisation des composés organiques présents dans les lixiviats de centres d'enfouissement technique de déchets ménagers. Le fractionnement de la matière organique est obtenu sur des colonnes type TSK PW, en élution eau pH 4 et eau-méthanol. La spectroscopie en UV-visible et en fluorescence, un détecteur évaporatif à diffusion de lumière sont utilisés pour la caractérisation des fractions. Cette méthode rapide de séparation associée à une multidétection permet une mise en évidence, dans les fractions issues de l'ultrafiltration, de composés organiques caractéristiques. Dans la fraction de poids moléculaires inférieurs à 1000 Daltons, trois familles sont détectées. Les substances humiques et les protéines sont les principaux groupes présents dans la fraction de poids moléculaires supérieurs à 10000 Daltons.
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Gaye-Seye, MD, G. Dodin, and JJ Aaron. "Étude par spectroscopie de fluorescence de l’effet du pH sur la complexation de la cinchonine par la β-lactoglobuline (β-LG)." Journal de Chimie Physique 94 (1997): 1208–15. http://dx.doi.org/10.1051/jcp/1997941208.

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Fanget, B., O. Devos, and E. Naffrechoux. "Rôle des acides humiques dans le transfert du pyrène entre les minéraux argileux et l'eau." Revue des sciences de l'eau 15 (April 12, 2005): 95–108. http://dx.doi.org/10.7202/705488ar.

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Les substances humiques se fixent en surface des minéraux argileux et modifient les sites d'adsorption des polluants organiques hydrophobes (POH). Parallèlement, les substances humiques dissoutes complexent les POH non ioniques selon des mécanismes de liaison réversible généralement évalués par le coefficient de partage Koc. Les solutés humiques seraient ainsi responsables du transport des POH dans les écosystèmes aquatiques par solubilisation de la fraction adsorbée sur le sédiment. Notre étude a pour objectif la quantification et la modélisation de ces phénomènes pour des composés modèles disponibles commercialement : kaolinite, pyrène et acide humique Aldrich purifié. L'isotherme d'adsorption de l'acide humique (AH) sur la kaolinite à pH 6,5 et à force ionique 10-2 M suit une loi de Freundlich, dont les valeurs du modèle sont KF =0,79 et nF =1,90. Dans les mêmes conditions expérimentales, le pyrène, composé modèle à quatre noyaux du groupe des hydrocarbures aromatiques polycycliques (HAP), semble s'adsorber en formant des microcristaux à la surface de l'argile. La présence d'acide humique fixé sur la kaolinite modifie l'adsorption du pyrène, qui se modélise par une isotherme de Freundlich de paramètres K'F =0,30 et n'F =2,00. Les interactions entre le pyrène et l'acide humique dissous ont été quantifiées par spectroscopie d'extinction de fluorescence et relation de Stern-Volmer. La fraction humique dissoute non adsorbée sur le minéral argileux se caractérise par de plus fortes valeurs de Koc que l'acide humique initial (2,95.105 L.kg-1 contre 1,86.105 L.kg-1). L'hypothèse de fractionnement des substances humiques lors de l'adsorption sur l'argile est confirmée par les variations du poids moléculaire moyen des AH, déterminé par chromatographie d'exclusion stérique, et des variations des intensités de fluorescence des fractions chromatographiées. Les résultats obtenus pour la quantification du pyrène désorbé du solide argileux dans l'eau pure et dans une solution d'AH (50 mg.L-1) tendent à minimiser le rôle de la matière organique naturelle dissoute dans le transfert des polluants hydrophobes du milieu solide au milieu liquide.
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Savickienė, Viktorija, Aurimas Bieliauskas, Sergey Belyakov, Eglė Arbačiauskienė, and Algirdas Šačkus. "Multicomponent Synthesis of New Fluorescent Boron Complexes Derived from 3-Hydroxy-1-phenyl-1H-pyrazole-4-carbaldehyde." Molecules 29, no. 14 (July 22, 2024): 3432. http://dx.doi.org/10.3390/molecules29143432.

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Novel fluorescent pyrazole-containing boron (III) complexes were synthesized employing a one-pot three-component reaction of 3-hydroxy-1-phenyl-1H-pyrazole-4-carbaldehyde, 2-aminobenzenecarboxylic acids, and boronic acids. The structures of the novel heterocyclic compounds were confirmed using 1H-, 13C-, 15N-, 19F-, and 11B-NMR, IR spectroscopy, HRMS, and single-crystal X-ray diffraction data. The photophysical properties of the obtained iminoboronates were investigated using spectroscopic techniques, such as UV–vis and fluorescence spectroscopies. Compounds display main UV–vis absorption maxima in the blue region, and fluorescence emission maxima are observed in the green region of the visible spectrum. It was revealed that compounds exhibit fluorescence quantum yield up to 4.3% in different solvents and demonstrate an aggregation-induced emission enhancement effect in mixed THF–water solutions.
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Hamon, Caroline, Solenn Reguer, Vérane Brisotto, Cécile Le Carlier de Veslud, Klet Donnart, Stéphane Blanchet, Yvan Pailler, Yoann Escats, and Ludovic Bellot-Gourlet. "Des outils de métallurgistes dans le Bronze ancien de Bretagne ? Révéler le rôle du macro-outillage lithique en associant analyses tracéologiques et de spectroscopie de fluorescence X." Bulletin de la Société préhistorique française 118, no. 4 (2021): 697–737. http://dx.doi.org/10.3406/bspf.2021.15250.

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The question of the emergence and development of copper and bronze metallurgies is a major issue in addressing the cultural and economic dynamics of the late Neolithic and early Bronze Age in Western Europe. This phenomenon is particularly relevant along the Atlantic coast, where the Armorican peninsula is central for better understanding how the control of these new productions contributed to the emergence of new elites and their chiefs, notably within the Armorican Tumulus culture (2150-1750 BC). However, evidence of Early Bronze Age mining activities and metallurgical production workshops remains scarce on the Atlantic coast, particularly in the Armorican Massif. The rare sources of copper ore are generally poor which have long suggested that the presence of metallic objects discovered in the rich burials of these periods, in particular in the tumuli of the Early Bronze Age, could only be the result of imports over more or less long distances. Recent work on the composition of some Armorican bronze objects has also highlighted a close proximity to ores from Ross Island in Ireland, suggesting that ores or ingots were imported from other regions, including across the Channel. Other works have nevertheless underlined that the exploitation of local copper veins could be envisaged in the Armorican Massif, especially as copper objects appear to concentrate in this area. Therefore, the discovery of direct or indirect evidence of copper metallurgy, particularly on settlement sites, is crucial for these periods. The presence of rare crucibles, moulds and ingots, as well as hearth structures and even furnaces, are precious clues for identifying possible episodes of metallurgical production. Stone tools constitute a second group of clues attesting metallurgical production throughout the Bronze Age, since they are now known to have been used at different stages of the operating chain, from the extraction and transformation of ores to the shaping and finishing of metal objects by hammering and abrading. This paper presents the results of a combination of use-wear and elementary X-ray fluorescence spectroscopy (XRF) analysis of a series of macrolithic tools from the Armorican Peninsula. The analyzed corpus comes from five sites in Britany dated by their pottery or by radiocarbon dating to the Early Bronze Age : it includes a settlement enclosure (Bel air, Lannion), an island settlement (Beg ar Loued, Molène) as well as less structured occupations however characteristic of the period (Kersulec, Plonéour-Lanvern ; la Colignière, Trémuson ; ZAC Kerisac, Plouisy). They illustrate the diversity of the contexts encountered dating to this period in the western part of the Armorican peninsula. For each of the sites, several tools were selected from a larger corpus of macrolithic tools, based on criteria of the raw materials, type of active surfaces and nature of the use-wear traces. The assemblage includes 19 macrolithic tools : 3 crushing-grinding tools, 2 tools with cupules, 1 pestle, 2 anvils, 3 hammerstones, 1 percussion or crushing tool, 3 heaps, 3 hammers and finally 2 sharpeners. The functional analysis method is based on the combination of the results of a low and high magnification use-wear analysis and elementary X-ray fluorescence (XRF) analyses, with two implemented measurement methods : portable X-ray fluorescence spectrometer (pXRF) and X-ray fluorescence mapping under Synchrotron radiation (Sy-XRF). The use-wear analysis alone therefore made it possible to exclude metallurgical use for 3 of the 13 tools analyzed, the other 10 tools showing traces at least linked to the working of mineral materials, with three quarters of them showing a combination of traces strongly suggesting the working of ore or metal. The combination of the use-wear and XRF analyses allows us to propose a use for ore processing or the transformation of metallic materials for 8 of these 10 tools. This result underlines that the hypotheses formulated following the use-wear analysis are well supported by the elementary residue analyses. The p-XRF analysis is able to detect copper residues even in relatively small quantities and to detect the heavier elements, such as tin. An average quantification is obtained, representing an analytical area of about 3 mm. Furthermore, the analytical precision permitted by Sy-XRF analysis, thanks in particular to the elemental distribution maps, with a selected resolution of a few tens of micrometers, makes it possible to distinguish chemical elements from the minerals making up the heterogeneous rocks generally used for macro-tools from the residues of processed materials that had catched on the surface. It also shows the correlations between the elementary distributions and the areas showing the traces of use. Due to the implementation modalities, the complementarity and concordance of the results of the Sy-XRF and p-XRF analyses with regard to the detection of copper residues is therefore stressed here. These analyses highlight the use of crushing tools, hammers and sharpeners for the working of copper alloy objects at all stages of the metal production and processing chain (production, shaping, maintenance). The identification of different types of metallurgists'' tools, some of them specialized, raises questions about the representation of this activity in a wide variety of Early Bronze Age contexts. It also underlines the question of the division of the operating chain in time and space, in particular between the extraction areas, primary metallurgy aimed at metal production and secondary metallurgy dedicated to the shaping or even finishing of the objects. The different occupation contexts from which these tools originate suggest a production of copper alloys, which despite its discretion in the archaeological record, must undoubtedly have been more important than initially envisaged. The presence of such tools in Armorican funerary contexts for the Early Bronze Age echoes these results.
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Anderson, T. Anthony, Jeon Woong Kang, Tatyana Gubin, Ramachandra R. Dasari, and Peter T. C. So. "Raman Spectroscopy Differentiates Each Tissue from the Skin to the Spinal Cord." Anesthesiology 125, no. 4 (October 1, 2016): 793–804. http://dx.doi.org/10.1097/aln.0000000000001249.

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Abstract Background Neuraxial anesthesia and epidural steroid injection techniques require precise anatomical targeting to ensure successful and safe analgesia. Previous studies suggest that only some of the tissues encountered during these procedures can be identified by spectroscopic methods, and no previous study has investigated the use of Raman, diffuse reflectance, and fluorescence spectroscopies. The authors hypothesized that real-time needle-tip spectroscopy may aid epidural needle placement and tested the ability of spectroscopy to distinguish each of the tissues in the path of neuraxial needles. Methods For comparison of detection methods, the spectra of individual, dissected ex vivo paravertebral and neuraxial porcine tissues were collected using Raman spectroscopy (RS), diffuse reflectance spectroscopy, and fluorescence spectroscopy. Real-time spectral guidance was tested using a 2-mm inner-diameter fiber-optic probe-in-needle device. Raman spectra were collected during the needle’s passage through intact paravertebral and neuraxial porcine tissue and analyzed afterward. The RS tissue signatures were verified as mapping to individual tissue layers using histochemical staining and widefield microscopy. Results RS revealed a unique spectrum for all ex vivo paravertebral and neuraxial tissue layers; diffuse reflectance spectroscopy and fluorescence spectroscopy were not distinct for all tissues. Moreover, when accounting for the expected order of tissues, real-time Raman spectra recorded during needle insertion also permitted identification of each paravertebral and neuraxial porcine tissue. Conclusions This study demonstrates that RS can distinguish the tissues encountered during epidural needle insertion. This technology may prove useful during needle placement by providing evidence of its anatomical localization.
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Delledonne, Chiara, Michela Albano, Tommaso Rovetta, Gianmarco Borghi, Mario Gentile, Anna Denia Marvelli, Piero Mezzabotta, et al. "Rediscovering the Painting Technique of the 15th Century Panel Painting Depicting the Coronation of the Virgin by Michele di Matteo." Heritage 7, no. 1 (January 10, 2024): 324–37. http://dx.doi.org/10.3390/heritage7010016.

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The study concerned a diagnostic spectroscopic campaign carried out on the panel painting depicting the Coronation of the Virgin (first half of the 15th century) by the late-Gothic Italian painter Michele di Matteo. The main aims were the identification of the original painting materials and the characterization of the painter’s artistic technique. A combined approach based on non- and micro-invasive techniques was employed. Visible and ultraviolet-induced fluorescence photography was used to select the areas of interest for spectroscopic analyses; X-ray radiography assessed the state of conservation of the support, while X-ray fluorescence and external reflection Fourier transform infrared spectroscopies allowed the chemical identification of pigments, binders, and varnishes. Attenuated total reflection infrared spectroscopy, optical microscopy, and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy were used to visualize and characterize the materials in the pictorial layers. The results highlighted the presence of pigments, possibly applied with an egg binder, consistent with the period of the production of the painting, as well as modern pigments used during subsequent restorations: an imprimitura with lead white and a gypsum-based ground layer. Concerning the gilding, the guazzo technique was confirmed by identifying a red bolo substrate and gold leaf.
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Dissertations / Theses on the topic "Spectroscopie de fluorescence"

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Aknine, Nathan. "Nouvelles sondes fluorescentes pour la bioimagerie des structures lipidiques cellulaires." Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAF035.

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Ce projet de doctorat vise la conception de sondes fluorescentes innovantes, pour une meilleure visualisation et compréhension des structures lipidiques cellulaires par bioimagerie. Ainsi, trois familles de nouvelles sondes fluorescentes ont été générées dans le cadre de cette thèse, visant à cibler et éclairer différentes localisations cellulaires. Premièrement, le ciblage covalent de la membrane plasmique par une stratégie nommée MemGraft, a permis de développer de nouveaux outils pour le marquage et la fonctionnalisation de la surface cellulaire. Ces sondes permettent un marquage robuste et efficace, une imagerie long-terme, un suivi de plusieurs populations par étiquetage fluorescent multiplexé, mais également la manipulation du comportement des cellules, sans porter atteinte à leur phénotype par ingénierie de la surface cellulaire. Ensuite, des sondes sensibles à leur environnement, basées sur un groupement trifluoroacétyle a permis l’imagerie de la polarité et de l’hétérogénéité des gouttelettes lipidiques intracellulaires. Cette stratégie a également rendu possible l’imagerie en super-résolution de la polarité de membranes intracellulaires. Finalement, nous avons exploré des stratégies de ciblage des organites par des sondes solvatochromes, en particulier la mitochondrie, mais également le réticulum endoplasmique, l’appareil de Golgi, ou encore les lysosomes, fournissant des informations sur l’organisation lipidique de leur membrane. En combinant chimie organique, chimie biologique, et microscopies de fluorescence, ces nouveaux outils ont été conçus et démontrent leur potentiel pour améliorer notre compréhension des structures et des processus cellulaires
This PhD project is focused on the design of innovative fluorescent probes for better visualization and understanding of cellular lipidic structures by bioimaging. Three families of novel fluorescent probes have been generated in this thesis, targeting and shedding light on different cellular localizations. First, the lipiddirected covalent targeting of the plasma membrane was introduced and enabled the development of new fluorescent tools for the labeling and functionalization of the cell surface. These probes allowed robust longterm cell imaging and barcoding as well as cell manipulation and cell surface engineering. Secondly, environment-sensitive probes based on a trifluoroacetyl electron acceptor were obtained, enabling the imaging of the polarity and heterogeneity of intracellular lipid droplets. This strategy also yielded probes for super-resolution imaging of the polarity in intracellular membranes. Finally, we explored the strategy for targeting organelles with solvatochromic probes, in particular mitochondria, but also the endoplasmic reticulum, Golgi apparatus and lysosomes, providing insights on the lipid organization of their membranes through polarity imaging. Combining organic chemistry, chemical biology, and fluorescence microscopy, these new advanced molecular tools have been obtained and showed their potential to improve our understanding of cellular structures and processes
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M'Baye, Gora Duportail Guy. "Sondes fluorescentes ratiométriques dérivées de la 3- Hydroxyflavone Etude spectroscopique de nouveaux dérivés et applications en biophysique membranaire /." Strasbourg : Université Louis Pasteur, 2007. http://eprints-scd-ulp.u-strasbg.fr:8080/755/01/MBAYE2007.pdf.

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Mohammadi, Shahla. "Etude par spectroscopie optique (FTIR, UV, Fluorescence) d'ADN." Paris 13, 1998. http://www.theses.fr/1998PA132013.

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Ce travail en spectroscopie optique (ftir, uv, fluorescence) a permis de determiner les conditions de formation, la stabilite, l'appariement des bases de double helices d'adn a brins paralleles et d'analyser la reconnaissance d'une sequence d'adn en double helice a partir de sites situes dans son grand sillon, d'une part en utilisant un oligonucleotide formant une triple helice d'autre part un intercalant muni de bras peptidiques. Le chapitre 1 porte sur les structures d'adn en double helices paralleles avec deux appariements differents. Le schema d'appariement trans-watson-crick connu pour les paires de bases a-t a ete complete par celui des paires g-c. Les paires g-c formees ont deux liaisons hydrogene. Les paires trans-watson-crick g-c et a-t ne sont pas isomorphes ce qui explique que la stabilite de la double helice parallele diminue quand le nombre de jonctions entre paires a-t et g-c augmente. La seconde structure parallele, limitee aux sequences homopurine-homopyrimidine, se forme avec un appariement hoogsteen a ph acide. Elle a ete stabilisee soit par des ions mg#2#+ soit par des intercalants (benzopyridoindoles). Ce meme type d'intercalant pourra stabiliser en orientation parallele par liaisons hoogsteen des oligonucleotides synthetiques sur une cible d'adn. Le chapitre 2 est consacre a l'etude de l'orientation du troisieme brin dans des triple helices ou la sequence du troisieme brin est centrosymetrique (g#3a#4g#3 et g#3t#4g#3). La fixation covalente de pyrene aux extremites des brins formant la triple helice a permis, par detection ou non de la formation d'excimere, de determiner l'orientation relative de ces brins. De plus l'etude de la cinetique de formation de la triple helice d(c#3t#4c#3)-d(g#3a#4g#3)#2 faite en marquant les deux brins cis-watson-crick suggere un mecanisme d'echange entre les brins homologues d(g#3a#4g#3). Le chapitre 3 concerne les complexes d'un derive de la porphyrine pourvue de bras arginyles (bap), avec des adn en double helices de differentes sequences. Les donnees de denaturation thermique en uv indiquent que bap, stabilise preferentiellement la double helice d(ggcgcc)#2 (que l'on retrouve dans le site pbs de hiv-1). Les spectres ir des complexes ont montre que bap, par ses bras peptidiques, interagit dans le grand sillon avec les sites n7 et o6 des guanines.
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Blancquaert, Yoann. "Nouvelles géométries optiques pour la Spectroscopie à Corrélation de Fluorescence." Phd thesis, Université Joseph Fourier (Grenoble), 2006. http://tel.archives-ouvertes.fr/tel-00148993.

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Le but initial de ce travail de thèse est de proposer une technique (basée sur la Fluorescence Correlation Spectroscopy, FCS) pour améliorer la sensibilité dans la discrimination de deux molécules ayant des constantes de diffusion proches. C'est dans ce contexte que nous avons étudié la FCCS (Fluorescence Cross-Correlation Spectroscopy). A défaut d'améliorer la sensibilité de la Spectroscopie à Correlation de Fluorescence nous avons proposé trois géométries de FCCS pour élargir le champ d'application de la corrélation de fluorescence.
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Ponsot, Alain. "Identification des tissus artériels par spectroscopie laser." Paris 12, 1991. http://www.theses.fr/1991PA120026.

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Avant de detruire au laser le tissu atheromateux, in vivo, le risque de perforation de l'artere doit etre elimine en identifiant le tissu se trouvant a l'extremite de la sonde a fibre optique. Afin d'identifier le tissu, nous avons etudie la fluorescence induite par laser des tissus arteriels. Nous avons pu montre que la fluorescence des tissus sains et atheromateux pouvait etre utilisee pour distinguer le tissu sain de la plaque atheromateuse. Un composant demultiplexeur en longueur d'onde a ete construit avec des voies d'analyse centrees sur des longueurs d'onde appropriees. Nous avons montre que ce composant pouvait etre utilise pour le diagnostic des tissus arteriels
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Colicchio, Bruno. "Déconvolution adaptative en microscopie tridimensionnelle de fluorescence." Mulhouse, 2004. http://www.theses.fr/2004MULH0779.

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Le microscope de fluorescence a pris une place importante pour l’étude du fonctionnement des cellules vivantes. Cependant, les données acquises ne sont pas directement exploitables en vu de mesures quantitatives car ces données subissent des distorsions. Une déconvolution est une solution au probl`eme. Des méthodes ont été proposées, mais requi`erent des connaissances de l’utilisateur pour le réglage de param`etres qui sont critiques, car ce probl`eme inverse est ’mal posé’ et une étape de régularisation est nécessaire. La premi`ere partie propose une automatisation du choix des param`etres de régularisation des méthodes directes. L’objectif est de permettre une utilisation de routine pour des non-spécialistes, permettant une constance des résultats ainsi qu’une stabilité accrue. Les méthodes directes automatisées ont été appliquées `a des images de cytologie et de cytogénétique moléculaire. Cependant, les déconvolutions nécessitent une bonne caractérisation du syst`eme, et une étude sur les param`etres susceptibles de varier entre plusieurs acquisitions est présentée. La seconde partie de cette th`ese propose un algorithme permettant la prise en compte de la non invariance du syst`eme, basé sur le processus de formation d’image. Par une approche Monte-Carlo, la solution est calculée par essais successifs aléatoires, en suivant une distribution de probabilité qui est fonction de l’erreur de biais entre l’estimation dans le plan ’image’ et l’image observée. La solution est obtenue en minimisant, par un algorithme de recuit simulé, une fonction d’erreur dans l’espace ’image’, avec contraintes sur le voisinage dans l’espace ’objet’
The 3D fluorescence microscope has become the method of choice in biological sciences for living cells study. However, the data acquired with conventional 3D fluorescence microscope are not quantitatively significant for spatial distribution or volume evaluation of fluorescent areas in reason of distortions. Deconvolution is a solution. Direct methods have been proposed, but knowledge of users for the tuning of critical parameters are required, because of the regularization needs due to the ill posed nature of the problem. The first part of this work present the automated tuning of direct methods regularization parameter. The aim is to permit the use of deconvolution by non specialists, giving constant results. The automated direct methods were applied on cytology and cytogenetic 3D data. The presented methods require a sharp characterization of the instrument, so an analysis of the influence of variation of the optical setup on the deconvolution result is presented. The last part of the work present concern the second tendency of deconvolution algorithms, by taking into account the space-variant response case. The solution is computed by a Monte-Carlo process, random guesses following a probability distribution function linked to the bias error of the estimation of the observed image and the raw observed image. The solution is obtained by the minimization of an error criterion in image space with neighborhood constraints in object space
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Sauvêtre, Alexandra. "Nouvelle méthode de révélation de traces papillaires par brumisation d'une solution fluorescente." Electronic Thesis or Diss., Sorbonne université, 2021. http://www.theses.fr/2021SORUS406.

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Les travaux présentés au sein de ce manuscrit de thèse visent à répondre à un besoin précis des forces de l’ordre et a ainsi consisté à déterminer s’il est possible ou non de rechercher sur de larges surfaces et de révéler des empreintes digitales directement sur scène de crime. Un procédé de révélation par spray couplé à une molécule fluorescente spécifique a été mis au point. Ce projet doctoral a consisté tout d’abord en un travail préliminaire visant à fabriquer des empreintes artificielles reproductibles. Ces dernières ont été utilisées pour les comparaisons entre empreintes révélées, qui prennent place dans l’évaluation de l’efficacité du procédé.Les propriétés spectroscopiques du fluorophore déposé par spray au sein des empreintes ont été étudiées afin de comprendre l’origine du contraste de fluorescence qui permet la visualisation de l’empreinte. Un autre aspect de la compréhension du mécanisme de révélation d’empreinte se situe au niveau du procédé lui-même, dans la mesure où le mode de fonctionnement du spray et le comportement des gouttes qu’il produit ont un impact conséquent sur les qualités d’empreintes révélées. Ainsi, ces derniers ont été étudiés via des techniques d’imageries pour mieux cerner et tenter d’optimiser le procédé de révélation par modification du solvant notamment. Enfin, le procédé proposé a été abordé sous un angle plus technique, afin de répondre aux enjeux opérationnels des forces de l’ordre
The thesis work aims at addressing a particular need of the police and establishing whether it was possible to visualise fingerprints or not by using a revelation process which is able to cover large surfaces. For reaching this goal, we studied in this work a new strategy: the spray deposition of a specifically designed fluorescent dye. The doctoral project consisted first in some preliminary work which aims at creating artificial and reproducible fingerprints. Those fingerprints will be used to compare revealed fingerprints with each other, in order to assess the effectiveness of the new method. The spectroscopic properties of the fluorophore used in this specific material have been studied, so as to have a better understanding of the mechanism taking places during revelation process. We also analysed the means of revelation itself, the spray and its functioning. Indeed, the drops produced by the spray have a fundamental impact on the quality of the fingerprints revealed. The spray and the drops produced have been studied by imaging techniques in order to better understand and try to optimize the revelation process by a substitution of solvent. Lastly, the method proposed has been considered from a technique angle, so as to meet operational challenges of the police on the crime scenes
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Lartia, Rémy. "Synthèse et études de sondes oligonucléotidiques dont le signal fluorescent est modifié au cours de l'hybridation." Orléans, 2004. http://www.theses.fr/2004ORLE2066.

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Le but de ce travail était de développer des sondes oligonucléotidiques liées à des marqueurs fluorescents émettant des signaux modifiés lors de l'hybridation avec les séquences complémentaires. Des conjugués ODNs-cyanine originaux ont été développés. L’influence de différents paramètres sur le signal fluorescent émis par la sonde ont été étudiés: position de liaison des marqueurs à l’ODN (5’ - ou internucléotidique), structure des cyanines, stéréochimie. Un nouveau réactif de phosphorylation des ODNs a été mis au point et utilisé pour la synthèse des conjugués marqués en position 5’. D’autres conjugués comportant à leur extrémité 5' deux marqueurs identiques possédant des propriétés intercalantes: thiazole orange, pyrène ou pérylène ont été obtenus. Les interactions entre les deux marqueurs varient lors de l’hybridation de la sonde avec la séquence complémentaire, induisant une modification du signal fluorescent dont la nature et l’intensité dépendent du marqueur considéré.
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Yushchenko, Dmytro. "Synthesis, spectroscopic study and application of ratiometric fluorescent dyes for apoptosis sensing and protein labelling." Strasbourg 1, 2007. http://www.theses.fr/2007STR13070.

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Grichine, Alexeï. "Imagerie spectrale confocale : développement et applications à l'étude des mécanismes d'action photodynamique de nouveaux sensibilisateurs." Orléans, 2002. http://www.theses.fr/2002ORLE2003.

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Un traitement local des tumeurs exige le développement de médicaments sélectifs et efficaces. C'est dans cette perspective que ce travail s'appuie sur la puissance de nouvelles méthodes optiques et notamment la technique d'imagerie spectrale confocale (ISC) de fluorescence et de diffusion Raman qui est ici présentée et développée. Cette technique permet de préciser la distribution et les propriétés d'une drogue en milieu cellulaire, à partir de ses spectres mesurés à l'aide d'un microscope confocal. De nouvelles approches d'étude de substances à potentiel anti-tumoral sont expérimentées in situ dans des cellules maintenues en survie et des coupes de tissus. Il est montré que cette même approche à base d'ISC peut être utilisée pour étudier les interactions et les mécanismes d'action biologique de médicaments fluorescents et même non-fluorescents, aux différents niveaux d'organisation du milieu vivant. Dans la deuxième partie de ce travail, une étude approfondie de nouveaux agents prometteurs pour les thérapies photodynamique (PDT) et catalytique binaire a été effectuée. Ces produits, notamment les sulfonates de phthalocyanine d'aluminium ; l'octacarboxyphthalocyanine de cobalt ; les sulfonates de phtalocyanine sans métal ; la 3-formyl-3-devinylchlorine p6 et la 131,151-N-(3-hydroxypropyl) cycloimide chlorine p6, ont été successivement analysés et optimisés afin d'établir les structures correspondant aux modes d'action les plus efficaces. Ainsi, une véritable stratégie de mise au point de médicament est proposée. Elle a permis, en particulier, de révéler une forte activité photodynamique des sulfonates de phthalocyanine sans métal, considérées jusqu'à présent sans intérêt thérapeutique et de développer de nouveaux photosensibilisateurs à base de chlorine p6 optimisés pour une absorption dans le rouge lointain et proche IR, fenêtre de transparence des tissus biologiques.
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Books on the topic "Spectroscopie de fluorescence"

1

Mary-Ann, Mycek, and Pogue Brian W, eds. Handbook of biomedical fluorescence. New York: Marcel Dekker, 2003.

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1940-, Schulman Stephen G., ed. Introduction to fluorescence spectroscopy. New York: Wiley, 1999.

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B, Thompson Richard, ed. Fluorescence sensors and biosensors. Boca Raton, FL: Taylor&Francis, 2005.

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Ammasi, Periasamy, and Clegg Robert M, eds. FLIM microscopy in biology and medicine. Boca Raton: Taylor & Francis, 2009.

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A, Borstad G., ed. Analysis of test and flight data from the fluorescence line imager. Ottawa: Dept. of Fisheries and Oceans, 1985.

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A, Borstad Gary, and Canada. Department ofFisheries and Oceans., eds. Analysis of test and flight data from the Fluorescence Line Imager. Ottawa: Fisheries and Oceans, Scientific Information and Publications Branch, 1985.

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E, Buhrke Victor, Jenkins Ron, and Smith, Deane K. (Deane Kingsley), eds. A practical guide for the preparation of specimens for x-ray fluorescence and x-ray diffraction analysis. New York: Wiley-VCH, 1998.

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Wolfbeis, Otto S., ed. Fluorescence Spectroscopy. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3.

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Rigler, Rudolf, and Elliot S. Elson. Fluorescence Correlation Spectroscopy. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59542-4.

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S, Wolfbeis Otto, ed. Fluorescence methods and applications: Spectroscopy, imaging, and probes. Malden, Mass: Blackwell Pub., 2008.

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Book chapters on the topic "Spectroscopie de fluorescence"

1

Schneckenburger, Herbert, Michael H. Gschwend, Karsten König, Reinhard Sailer, and Wolfgang S. L. Strauß. "Fluorescence Lifetime Imaging and Spectroscopy in Photobiology and Photomedicine." In Fluorescence Microscopy and Fluorescent Probes, 71–78. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_8.

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Schulman, Steven G. "Fluorescence Spectroscopy: Where We Are and Where We’re Going." In Fluorescence Spectroscopy, 3–12. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_1.

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Lakowicz, Joseph R., Henryk Szmacinski, Kazimierz Nowaczyk, Klaus W. Berndt, and Michael L. Johnson. "Fluorescence Lifetime Imaging and Application to Ca2+ Imaging." In Fluorescence Spectroscopy, 129–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_10.

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Hermetter, A., E. Prenner, J. Loidl, E. Kalb, A. Sommer, and F. Paltauf. "Ether Phospholipids in Membranes: Applications of Phase and Steady-State Fluorometry." In Fluorescence Spectroscopy, 149–57. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_11.

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Kinnunen, Paavo K. J., Anu Koiv, and Pekka Mustonen. "Pyrene-Labelled Lipids as Fluorescent Probes in Studies on Biomembranes and Membrane Models." In Fluorescence Spectroscopy, 159–71. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_12.

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Slavík, Jan. "Optical Detection of Intracellular Ion Concentrations." In Fluorescence Spectroscopy, 173–85. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_13.

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Miller, James N., Marc B. Brown, Nichola J. Seare, and Stephen Summerfield. "Analytical Applications of Very Near-IR Fluorimetry." In Fluorescence Spectroscopy, 189–96. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_14.

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Valcárcel, M., and M. D. Luque de Castro. "Fluorescence Detection in Flow Injection Analysis." In Fluorescence Spectroscopy, 197–212. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_15.

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Goldberg, Marvin C., and Eugene R. Weiner. "Fluorescence Spectroscopy in Environmental and Hydrological Sciences." In Fluorescence Spectroscopy, 213–41. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_16.

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Klein, Christian, Hans-Georg Batz, Brigitte Draeger, Hans-Joachim Guder, Rupert Herrmann, Hans-Peter Josel, Ulrich Nägele, Roland Schenk, and Bernd Vogt. "Fluorescence Polarisation Immunoassay." In Fluorescence Spectroscopy, 245–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3_17.

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Conference papers on the topic "Spectroscopie de fluorescence"

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Bakeev, Katherine, Jacopo Zini, Amutha Daniel, and Mari Tenhunen. "Time-Gated Raman for Bioprocess Analysis." In Applied Industrial Spectroscopy, AM1A.2. Washington, D.C.: Optica Publishing Group, 2024. https://doi.org/10.1364/ais.2024.am1a.2.

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Raman spectroscopy for process monitoring can be limited due to interference of sample-induced fluorescence overwhelming the Raman signal. Using a Timegated<sup>®</sup> Raman spectrometer overcomes fluorescence and we show the successful monitoring of biopharmaceutical product production. Full-text article not available; see video presentation
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Kosterev, Anatoliy A. "Exploring Laser-Induced Fluorescence as a Process Analytics Method for Dissolved Oxygen." In Applied Industrial Spectroscopy, ATu1G.3. Washington, D.C.: Optica Publishing Group, 2024. https://doi.org/10.1364/ais.2024.atu1g.3.

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Laser-induced fluorescence of oxygen in organic solvents is considered for a contactless process analytics method. Neither a photosensitizer nor singlet oxygen traps were added to solvents. Singlet oxygen self-quenching and solvent contamination effects reported.
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Sowoidnich, Kay, Stefan Pätzold, Markus Ostermann, Martin Maiwald, and Bernd Sumpf. "Portable Shifted Excitation Raman Difference Spectroscopy: Towards Qualitative and Quantitative On-site Soil Analysis." In Applied Industrial Spectroscopy, AW3A.5. Washington, D.C.: Optica Publishing Group, 2024. https://doi.org/10.1364/ais.2024.aw3a.5.

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Portable shifted excitation Raman difference spectroscopy effectively overcomes fluorescence and ambient light interference during on-site soil investigations. This enables to identify selected soil components and to predict the contents of carbonate and soil organic carbon.
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Wagner, Christoph. "Spectroscopy and Disinfection Byproducts in Water Treatment." In Applied Industrial Spectroscopy, AW3A.4. Washington, D.C.: Optica Publishing Group, 2024. https://doi.org/10.1364/ais.2024.aw3a.4.

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This study presents UV/VIS and fluorescence measurements to predict disinfection byproducts in drinking water. Data indicates good correlations, highlighting these techniques' potential for real-time monitoring and improving water safety and quality control. Full-text article not available; see video presentation
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Borgese, Laura, Fabjola Bilo, Elza Bontempi, Luca Seralessandri, Amedeo Cinosi, Giacomo Siviero, and Laura E. Depero. "Total Reflection X-Ray Fluorescence (TXRF) spectroscopy for environmental and biological analysis. - Réflexion spectroscopie des rayons X de fluorescence totale dans l'analyse biologique et de l'environnement." In 16th International Congress of Metrology. Les Ulis, France: EDP Sciences, 2013. http://dx.doi.org/10.1051/metrology/201310010.

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Paithankar, D. Y., and E. M. Sevick-Muraca. "Fluorescence lifetime imaging with frequency-domain photon migration measurement." In Biomedical Optical Spectroscopy and Diagnostics. Washington, D.C.: Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.fg3.

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The feasibility of employing fluorescent contrast agents to perform optical imaging in tissues and other scattering media has been examined through computational studies. Fluorescence lifetime and yield can give crucial information about local metabolite concentration or environmental conditions within tissues. This information can be employed towards disease detection, diagnosis, and treatment if non- invasively quantitated from re-emitted optical signals. However, the problem of inverse image reconstruction of fluorescence yield and lifetime is complicated due to the highly scattering nature of the tissue. In this work, a light propagation model employing the diffusion equation is used to account for the scattering of both the excitation and fluorescent light. Simulated measurements of frequency-domain parameters of fluorescent modulated AC intensity and phase-lag are used as inputs to an inverse image reconstruction algorithm which employs the diffusion model to relate frequency-domain measurements resulting from a modulated input at the phantom periphery. In the inverse image reconstruction algorithm, we employ a Newton-Raphson technique combined with Marquardt algorithm to converge upon the fluorescent properties within the medium. The successful reconstruction of both the fluorescence yield and lifetime in the case of heterogeneous fluorophore distribution within a scattering medium has been demonstrated without a priori information or without the necessity of obtaining "absence" images.
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Pepich, Barry V., James B. Callis, and Martin Gouterman. "Pulsed free jet expansion system for high resolution fluorescence spectroscopy of capillary GC effluents: application to a complex environmental sample." In International Laser Science Conference. Washington, D.C.: Optica Publishing Group, 1986. http://dx.doi.org/10.1364/ils.1986.tue6.

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A new hyphenated instrument for the detection of fluorescent polynuclear aromatic hydrocarbons in complex enviromental samples is described. The instrument combines a high resolution separation tool, capillary gas chromatography, with a high resolution spectroscopic instrument, laser fluorescence excitation spectroscopy. Optimal interfacing of these two devices is accomplished by means of a novel pulsed free jet expansion system which withstands temperatures to 300°C, has a very low dead volume, pro vides an excellent duty factor match between the steady-state gas chromatograph and the pulsed laser, and yields cooled, isolated gas phase molecules for high resolution spectroscopy. As a test of the system, a complex environmental sample was analyzed for the geometric isomers of monomethylanthracene.
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Rakestraw, David J. "Applications of Infrared Spectroscopy for Combustion Diagnostics." In Laser Applications to Chemical and Environmental Analysis. Washington, D.C.: Optica Publishing Group, 1996. http://dx.doi.org/10.1364/lacea.1996.lthc.1.

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The application of infrared spectroscopies for combustion diagnostics offers an exciting new opportunity to measure a wide range of polyatomic molecular species. The ability to measure low concentrations of many species important in combustion chemistry has been limited to a great extent to molecules that that can be probed using laser-induced fluorescence in the visible and ultraviolet spectral regions. Over the past several years our group has been investigating the use of infrared spectroscopies in an effort to greatly expand the polyatomic molecules that can be probed using laser diagnostics. This effort has benefited from advances in laser technology as well as the development of new spectroscopic approaches. The work discussed in this paper makes use of a tunable single-mode optical parametric oscillator system to provide high resolution rovibrational spectra. Degenerate four-wave mixing (DFWM) and cavity ringdown laser absorption spectroscopy (CRLAS) are the two spectroscopic techniques investigated.
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Bardeen, C. J., and C. V. Shank. "Solvent Dependence of the Femtosecond Three Pulse Photon Echo for LD690 in n-Alcohols." In International Conference on Ultrafast Phenomena. Washington, D.C.: Optica Publishing Group, 1994. http://dx.doi.org/10.1364/up.1994.thd.16.

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The characteristic timescales of the solvent-solute interaction are of great interest to those studying the spectroscopy and reaction dynamics of molecules in condensed phases. These timescales influence a variety of linear and non-linear spectroscopic measurements, and together these different types of measurements should constrain the quantitative values of the interaction parameters. In this work we use both linear (absorption and fluorescence) and non-linear (resonance raman and femtosecond time-resolved 4-wave-mixing) spectroscopies to systematically investigate the spectral broadening of the dye molecule LD690 in a series of n-alcohols.
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Obeidat, A. T., A. E. Kaplan, J. B. Khurgin, and M. D. Stem. "Single-Fiber Optical Probe for Two-Photon Induced Fluorescence of Biological Markers." In Biomedical Optical Spectroscopy and Diagnostics. Washington, D.C.: Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.ft8.

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The use of fluorescent dyes which can be attached to specific cellular components as biological markers has become a well- established biomedical technique in recent years [1]. When optically stimulated, these dyes emit a characteristic fluorescence, enabling the structure and organization of the stained sample to be readily studied and visualized by means of fluorescence spectroscopy and confocal microscopy [2]. Moreover, some dyes exhibit enhanced fluorescence if attached to certain biological molecules. For instance, 4’,6- diamidino-2-phenylindole (DAPI) exhibits such enhanced fluorescence when attached to the AT base pairs on a DNA strand [3], Such properties are useful for mapping DNA structure or identifying the presence of particular molecules in a sample.
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Reports on the topic "Spectroscopie de fluorescence"

1

Fernando, P. U. Ashvin Iresh, Gilbert Kosgei, Matthew Glasscott, Garrett George, Erik Alberts, and Lee Moores. Boronic acid functionalized ferrocene derivatives towards fluoride sensing. Engineer Research and Development Center (U.S.), July 2022. http://dx.doi.org/10.21079/11681/44762.

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In this technical report (TR), a robust, readily synthesized molecule with a ferrocene core appended with one or two boronic acid moieties was designed, synthesized, and used toward F- (free fluoride) detection. Through Lewis acid-base interactions, the boronic acid derivatives are capable of binding with F- in an aqueous solution via ligand exchange reaction and is specific to fluoride ion. Fluoride binding to ferrocene causes significant changes in fluorescence or electrochemical responses that can be monitored with field-portable instrumentation at concentrations below the WHO recommended limit. The F- binding interaction was further monitored via proton nuclear magnetic resonance spectroscopy (1H-NMR). In addition, fluorescent spectroscopy of the boronic acid moiety and electrochemical monitoring of the ferrocene moiety will allow detection and estimation of F- concentration precisely in a solution matrix. The current work shows lower detection limit (LOD) of ~15 μM (285 μg/L) which is below the WHO standards. Preliminary computational calculations showed the boronic acid moieties attached to the ferrocene core interacted with the fluoride ion. Also, the ionization diagrams indicate the amides and the boronic acid groups can be ionized forming strong ionic interactions with fluoride ions in addition to hydrogen bonding interactions.
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Scalettar, Bethe A. Fluorescence spectroscopic studies of DNA dynamics. Office of Scientific and Technical Information (OSTI), April 1987. http://dx.doi.org/10.2172/10150990.

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VerMeulen, Holly, Jay Clausen, Ashley Mossell, Michael Morgan, Komi Messan, and Samuel Beal. Application of laser induced breakdown spectroscopy (LIBS) for environmental, chemical, and biological sensing. Engineer Research and Development Center (U.S.), June 2021. http://dx.doi.org/10.21079/11681/40986.

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The Army is interested in sensors capable of characterizing/monitoring the environment (battlefield or military training ranges) at proximal distances. Recently, we evaluated laser induced breakdown spectroscopy (LIBS) systems (hand-held, proximal, and bench top) for the characterization of metals (antimony, copper, lead, tungsten, and zinc) in soils obtained from military training ranges. We then compared the results to findings obtained with standard field and laboratory instrumentation for metals analysis -X-ray Fluorescence (XRF) and Inductively Couple Plasma- Optical Emission Spectroscopy (ICP-OES).
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DeRose, Paul C. Recommendations and guidelines for standardization of fluorescence spectroscopy. Gaithersburg, MD: National Institute of Standards and Technology, 2007. http://dx.doi.org/10.6028/nist.ir.7457.

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McKnight, Diane. Fulvic Acid Oxidation State Detection Using Fluorescence Spectroscopy. Fort Belvoir, VA: Defense Technical Information Center, August 2002. http://dx.doi.org/10.21236/ada412483.

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Mukerji, I. Fluorescence spectroscopy of excitation transfer in Photosystem 1. Office of Scientific and Technical Information (OSTI), December 1990. http://dx.doi.org/10.2172/5871906.

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Clausen, Jay, Richard Hark, Russ Harmon, John Plumer, Samuel Beal, and Meghan Bishop. A comparison of handheld field chemical sensors for soil characterization with a focus on LIBS. Engineer Research and Development Center (U.S.), February 2022. http://dx.doi.org/10.21079/11681/43282.

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Commercially available handheld chemical analyzers for forensic applications have been available for over a decade. Portable systems from multiple vendors can perform X-ray fluorescence (XRF) spectroscopy, Raman spectroscopy, Fourier transform infrared(FTIR) spectroscopy, and recently laser-induced breakdown spectroscopy (LIBS). Together, we have been exploring the development and potential applications of a multisensor system consisting of XRF, Raman, and LIBS for environmental characterization with a focus on soils from military ranges. Handheld sensors offer the potential to substantially increase sample throughput through the elimination of transport of samples back to the laboratory and labor-intensive sample preparation procedures. Further, these technologies have the capability for extremely rapid analysis, on the order of tens of seconds or less. We have compared and evaluated results from the analysis of several hundred soil samples using conventional laboratory bench top inductively coupled plasma atomic emission spectroscopy (ICP-AES) for metals evaluation and high-performance liquid chromatography (HPLC) and Raman spectroscopy for detection and characterization of energetic materials against handheld XRF, LIBS, and Raman analyzers. The soil samples contained antimony, copper, lead, tungsten, and zinc as well as energetic compounds such as 2,4,6-trinitrotoluene(TNT), hexahydro-1,3,5-triazine (RDX), nitroglycerine (NG), and dinitrotoluene isomers (DNT). Precision, accuracy, and sensitivity of the handheld field sensor technologies were compared against conventional laboratory instrumentation to determine their suitability for field characterization leading to decisional outcomes.
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Zhu, Changfang, and Nirmala Ramanujam. Diagnosis of Breast Cancer Using Fluorescence and Reflectance Spectroscopy. Fort Belvoir, VA: Defense Technical Information Center, September 2004. http://dx.doi.org/10.21236/ada433953.

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McGown, L. B. Studies of multifrequency phase-resolved fluorescence spectroscopy for spectral fingerprinting. Office of Scientific and Technical Information (OSTI), January 1990. http://dx.doi.org/10.2172/6383290.

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Shear, Trevor A. Review of X-ray Tomography and X-ray Fluorescence Spectroscopy. Office of Scientific and Technical Information (OSTI), March 2017. http://dx.doi.org/10.2172/1351176.

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