Relevant bibliographies by topics / Sperm cells

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Sperm cells'

Author: Grafiati
Published: 4 June 2021
Last updated: 2 November 2024

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Journal articles on the topic "Sperm cells"

1

Kurd, Soleiman, Sara Hosseini, Fardin Fathi, Vahid Jajarmi, and Mohammad Salehi. "Dimethyl sulphoxide and electrolyte-free medium improve exogenous DNA uptake in mouse sperm and subsequently gene expression in the embryo." Zygote 26, no. 5 (October 2018): 403–7. http://dx.doi.org/10.1017/s0967199418000436.

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SummaryOne of the methods to generate transgenic animals is called sperm-mediated gene transfer (SMGT). Mature sperm cells can take up exogenous DNA molecules intrinsically and transfer them into the oocyte during fertilization. This study assessed the effect of dimethyl sulfoxide (DMSO) and electrolyte-free medium (EFM) on DNA uptake (EGFP–N1plasmid) in mouse sperm. Sperms cells cultured in human tubular fluid (HTF) without any treatment were considered as the control group. Sperms cells that were incubated in EFM and HTF with DNA/DMSO at 4°C were classified into EFM and HTF groups. Sperm motility and viability were assessed following treatment. In vitro fertilization (IVF) with sperm in all groups was performed. Fertilization, embryo development and GFP-positive blastocyst rates were analyzed and compared. The result showed that sperm motility and viability in EFM were better than those in the HTF group. The rate of development to reach the blastocyst stage and GFP-positive blastocysts was significantly higher in the EFM group compared with the HTF group (P<0.05). Our data demonstrate that sperm stored in the EFM group can improve the efficiency of SMGT for the generation of GFP-positive blastocysts.
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Belayeva, N. S. "Relation of the male gametes with embryo sec cells. The hypothesis of double fertilization." Acta Societatis Botanicorum Poloniae 50, no. 1-2 (2014): 173–75. http://dx.doi.org/10.5586/asbp.1981.026.

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The sperms one after another get out of synergids. The front sperm gets the first into the egg cell attraction zone and then the sperm comes into contact with egg membrane. At this moment Attraction ceases and the second sperm is led by a current of cytoplasma to the central nucleus. In the egg cell the sperm nucleus is led to the nucleus by cytoplasmic current too. After fertilization the character of cytoplasmic motion changes, because of a cell membrane damage. The presence of the sperm in the female nuclei may also serve as a regulating factor.
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Xiong, Hui, Xiangchen Li, Qingyun Hu, Pengfei Hu, and Weijun Guan. "Chicken Model for Inducing Primordial Germ Cells Differentiating into Motile Sperms in vitro." Avian Biology Research 10, no. 1 (February 2017): 12–18. http://dx.doi.org/10.3184/175815617x14799886572986.

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Stem cell technologies have been widely used in the study of spermatogenesis. However, deriving motile sperm from stem cells in vitro is still rarely achieved. We found that chicken primordial germ cells could directly differentiate into sperm by using retinoic acid in a non-testicular culture system. The induced sperms were characterised by RT-PCR, immunofluorescence and flow cytometry techniques. Results suggested that chicken primordial germ cells could produce motile sperm in vitro. Our work has provided a novel animal model of spermatogenesis in vitro, which might be used for male reproductive mechanism research.
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Bukowska, D., B. Kempisty, J. Sikora, M. Jackowska, M. Wozna, P. Antosik, H. Piotrowska, J. Budna, and JM Jaskowski. "The effect of swim-up purification and incubation of cells on sperm viability in dogs of different ages." Veterinární Medicína 56, No. 5 (June 10, 2011): 248–54. http://dx.doi.org/10.17221/1560-vetmed.

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The influence of selected semen extenders on the motility of frozen-thawed dog spermatozoa has been clearly demonstrated in several studies, although there are no reports indicating the effect of swim-up purification on sperm viability in this species of mammals. Therefore, this study was aimed at investigating phosphatidylserine (PS) externalization and necrosis in sperm after variable lengths of time of in vitro incubation after swim-up purification. Dog semen samples were collected from (i) ten dogs aged six months to 1.5 year, (ii) ten dogs aged six to eight years, and (iii) ten dogs aged 11 to 13 years. A flow cytometric method was employed to evaluate dog sperm viability in animals of different age groups after employment of a swim-up (SU) purification technique. After SU spermatozoa were incubated for 15, 30, 45 and 60 min in Sperm-TALP medium. We observed an increase in the number of viable sperm (double negatives) after 15 min of incubation compared to sperm undergoing PS externalization and late necrotic sperms (P &lt; 0.001) in each group of dogs. We also found a higher number of early necrotic sperm after 60 min of in vitro incubation (P &lt; 0.001). The amounts of late necrotic sperm and cells with PS externalization were similar among animals of different age groups. We show for the first time that most viable sperm are recovered after an in vitro incubation step of 15 min (control samples in this study) because as the time of incubation increases so does the number of degenerated or damaged cells. The higher number of early necrotic cells after 60 min of in vitro incubation may be a special feature of this species and may result from the induction of necrosis in the sperm. This knowledge may be used in future experiments for the preparation of spermatozoa following in vitro fertilization in dogs.
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Xie, Lan, Rui Ma, Chao Han, Kai Su, Qiufang Zhang, Tian Qiu, Lei Wang, et al. "Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device." Clinical Chemistry 56, no. 8 (August 1, 2010): 1270–78. http://dx.doi.org/10.1373/clinchem.2010.146902.

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BACKGROUND Sperm screening is an essential step in in vitro fertilization (IVF) procedures. The swim-up method, an assay for sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis, are also important indicators of mammalian sperm health. To monitor both sperm motility and chemotaxis simultaneously during sperm screening, we designed and constructed a microdevice comprising a straight channel connected with a bibranch channel that mimics the mammalian female reproductive tract. METHODS The width and length of the straight channel were optimized to select the motile sperms. We selectively cultured cumulus cells in the bibranch channel to generate a chemoattractant-forming chemical gradient. Sperm chemotaxis was represented by the ratio of the sperm swimming toward different branches. RESULTS The percentage of motile sperms improved from 58.5% (3.8%) to 82.6% (2.9%) by a straight channel 7 mm in length and 1 mm in width. About 10% of sperms were found to be chemotactically responsive in our experiment, which is consistent with previous studies. CONCLUSIONS For the first time, we achieved the combined evaluation of both sperm motility and chemotaxis. The motile and chemotactically responsive sperms can easily be enriched on a lab-on-a-chip device to improve IVF outcome.
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L Zanella, Eraldo. "Melatonin Effect on Cryopreserved Sperm Cells of Crioulo Stallions." Open Access Journal of Veterinary Science & Research 5, no. 2 (2020): 1–8. http://dx.doi.org/10.23880/oajvsr-16000202.

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The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation.
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Ding, Qiang, Xiuhu Ding, Shuwen Xia, Fang Zhao, Kunlin Chen, Yong Qian, Shaoxian Cao, et al. "Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility." Genes 13, no. 10 (October 3, 2022): 1788. http://dx.doi.org/10.3390/genes13101788.

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MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted ATP2A2 mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining ATP2A2 expression. Moreover, we observed high calcium concentrations and high ATP2A2 protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting ATP2A2 of the calcium signaling pathway in bovine spermatozoa.
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Kondracki, Stanisław, Anna Wysokińska, Magdalena Kania, and Krzysztof Górski. "Application of two staining methods for sperm morphometric evaluation in domestic pigs." Journal of Veterinary Research 61, no. 3 (September 26, 2017): 345–49. http://dx.doi.org/10.1515/jvetres-2017-0045.

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Abstract Introduction: The effect of two smear staining methods on the dimensions and shape of sperm cells in the semen of domestic pigs was evaluated. Material and Methods: The studies were carried out on 30 ejaculates collected from 15 boars, which included five Duroc boars, five Pietrain boars, and five hybrid Duroc × Pietrain boars. Each ejaculate was next sampled to make two microscopic slides, of which one was stained with eosin-nigrosin and the other with eosin-gentian dye. In total, 600 measurements of sperm cells were made. Each sperm was measured for the following morphometric parameters: head length, head width, head area, head perimeter, tail length, and the total sperm length. Results: Sperms measured on slides stained with eosin-nigrosin showed lower dimensions as compared with those stained with the eosin-gentian dye method. Sperm stained with eosin-nigrosin had shorter and narrower heads than sperm stained with eosin-gentian dye. The method of staining, therefore, affected not only the dimensions of the sperm, but also the proportions of the dimensions defining the shape of the sperm. Conclusions: The size and shape parameters in porcine sperm may take on different values depending on the method of semen staining. Sperm cells stained with eosin-nigrosin are smaller than the sperm stained with eosin-gentian dye. The sensitivity of the sperm to the type of dye used for the fixation may be associated with genetic factors.
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Khodamoradi, Maedeh, Saeed Rafizadeh Tafti, Seyed Ali Mousavi Shaegh, Behrouz Aflatoonian, Mostafa Azimzadeh, and Patricia Khashayar. "Recent Microfluidic Innovations for Sperm Sorting." Chemosensors 9, no. 6 (June 1, 2021): 126. http://dx.doi.org/10.3390/chemosensors9060126.

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Sperm selection is a clinical need for guided fertilization in men with low-quality semen. In this regard, microfluidics can provide an enabling platform for the precise manipulation and separation of high-quality sperm cells through applying various stimuli, including chemical agents, mechanical forces, and thermal gradients. In addition, microfluidic platforms can help to guide sperms and oocytes for controlled in vitro fertilization or sperm sorting using both passive and active methods. Herein, we present a detailed review of the use of various microfluidic methods for sorting and categorizing sperms for different applications. The advantages and disadvantages of each method are further discussed and future perspectives in the field are given.
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Bhat, Ghulam Rasool, Farooz Ahmad Lone, and Nafis Ibni Assad. "Spermbots: A Promising Futuristic Innovation in Assisted Reproductive Technology." Indian Journal of Animal Reproduction 44, no. 2 (December 29, 2023): 14–17. http://dx.doi.org/10.48165/ijar.2023.44.02.3.

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Spermbots are robotic sperms formed out of sperm cells conjugated to artificial microstructures, having potential applications ranging from biomedical processes, drug delivery systems, in situ real time imagery and assisted reproduction. The robotic sperm can act as an exploratory microdevice in biological networks. Incorporation of a biological entity like sperm into microstructures under the environment of magnetic or electric fields helps in shape templating and carrying chemotherapeutic agents to target sites. Besides its role in drug delivery systems, spermbots can potentially contribute in combating infertility, especially in oligo-zoospermia and necro-zoospermia in males. Numerous checkpoints may impede sperm cells to reach the oocyte in vivo. Spermbots bypass these sites and carry sperm to oocyte. Targeted delivery always requires interventions of natural functional aspects of living systems. Sperm flagellum, being a biological motor in nature, can be harnessed as a driving force in spermbots to ensure delivery of fertile sperm cells with impaired motile machinery to the target. Moreover, the technology has a potential to unravel sperm migration patterns and understanding the processes in vivo. After a review of documented literature on possible use of spermbot technology in assisted reproduction, we hereby discuss the application of this new and innovative technology in humans and animals. The paper also highlights certain shortfalls in the widespread application of this cargo delivery technology in assisted reproduction.
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Dissertations / Theses on the topic "Sperm cells"

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Friedrich, Benjamin. "Chemotaxis of Sperm Cells." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1235056439247-79608.

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Sperm cells are guided to the egg by chemoattractants in many species. Sperm cells are propelled in a liquid by the regular beat of their flagellum. In the presence of a concentration gradient of a chemoattractant, they can steer upwards the concentration gradient, a process called chemotaxis. Eggs release chemoattractants to guide the sperm cells to the egg. Sperm chemotaxis is best studied experimentally in the sea urchin. There, specific receptors in the flagellar membrane of the sperm cells are activated upon binding of chemoattractant molecules and trigger a signaling cascade which ultimately changes the activity of the molecular motors which drive the flagellar beat and result in a swimming response. Sea urchin sperm cells swim along circular and helical paths. Sperm cells of the sea urchin and several other species swim along helical paths far from boundary surfaces in the absence of chemoattractant. In a two-dimensional experimental geometry, sperm swimming paths are planar circles. The non-zero curvature of their swimming paths is a direct consequence of an asymmetry of their flagellar beat. In a concentration gradient of chemoattractant, sperm swimming path are drifting circles in two dimensions and bend helices in three dimensions. What is the working mechanism of sperm chemotaxis? In this thesis, we develop a theoretical description of sperm chemotaxis which can be subsumed as follows: While swimming along an approximately circular path in a concentration gradient a sperm cell traces a periodic concentration stimulus from the concentration field that has the frequency of circular swimming. The chemotactic signaling system processes this stimulus and causes a periodic modulation of the curvature of the swimming path which then gives rise to a swimming path which is a drifting circle. The relative direction of the drift with respect to the gradient direction is determined by the phase shift between the stimulus and the curvature oscillations. This picture is in perfect agreement with recent experimental findings. The mechanism is more general and also works in three dimensions for swimming along helical paths. Our results. Our theoretical description of sperm chemotaxis clarifies the concepts underlying sperm chemotaxis. In particular, we derive the role of internal timing of the chemotactic signaling system for sperm chemotaxis. We conclude that sampling a concentration field along circular and helical paths is a robust strategy for chemotaxis that does not require fine-tuning of parameters and which works reliable also in the presence of fluctuations. In a last chapter of this thesis, we discuss sperm chemotaxis in the more general context of an abstract search problem.
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Friedrich, Benjamin. "Chemotaxis of Sperm Cells." Doctoral thesis, Technische Universität Dresden, 2008. https://tud.qucosa.de/id/qucosa%3A23708.

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Sperm cells are guided to the egg by chemoattractants in many species. Sperm cells are propelled in a liquid by the regular beat of their flagellum. In the presence of a concentration gradient of a chemoattractant, they can steer upwards the concentration gradient, a process called chemotaxis. Eggs release chemoattractants to guide the sperm cells to the egg. Sperm chemotaxis is best studied experimentally in the sea urchin. There, specific receptors in the flagellar membrane of the sperm cells are activated upon binding of chemoattractant molecules and trigger a signaling cascade which ultimately changes the activity of the molecular motors which drive the flagellar beat and result in a swimming response. Sea urchin sperm cells swim along circular and helical paths. Sperm cells of the sea urchin and several other species swim along helical paths far from boundary surfaces in the absence of chemoattractant. In a two-dimensional experimental geometry, sperm swimming paths are planar circles. The non-zero curvature of their swimming paths is a direct consequence of an asymmetry of their flagellar beat. In a concentration gradient of chemoattractant, sperm swimming path are drifting circles in two dimensions and bend helices in three dimensions. What is the working mechanism of sperm chemotaxis? In this thesis, we develop a theoretical description of sperm chemotaxis which can be subsumed as follows: While swimming along an approximately circular path in a concentration gradient a sperm cell traces a periodic concentration stimulus from the concentration field that has the frequency of circular swimming. The chemotactic signaling system processes this stimulus and causes a periodic modulation of the curvature of the swimming path which then gives rise to a swimming path which is a drifting circle. The relative direction of the drift with respect to the gradient direction is determined by the phase shift between the stimulus and the curvature oscillations. This picture is in perfect agreement with recent experimental findings. The mechanism is more general and also works in three dimensions for swimming along helical paths. Our results. Our theoretical description of sperm chemotaxis clarifies the concepts underlying sperm chemotaxis. In particular, we derive the role of internal timing of the chemotactic signaling system for sperm chemotaxis. We conclude that sampling a concentration field along circular and helical paths is a robust strategy for chemotaxis that does not require fine-tuning of parameters and which works reliable also in the presence of fluctuations. In a last chapter of this thesis, we discuss sperm chemotaxis in the more general context of an abstract search problem.
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Tam, Wing-hei Winky. "Adrenomedullin in oviduct and sperm function." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B39430248.

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Tam, Wing-hei Winky, and 譚詠曦. "Adrenomedullin in oviduct and sperm function." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39430248.

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Sriboonlert, Ajaraporn. "Proteome and transcriptional analysis of Arabidopsis thaliana sperm cells." Thesis, University of Bath, 2008. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538168.

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Angiosperm sexual reproduction is unique and remarkable. Unlike for other living organisms, fertilisation involves the fusion of two sperm cells to two cell types of female gametophyte, the egg and central cell. This fertilization process is called double fertilisation. Fusion of the egg and the sperm yields a zygote whereas the interaction of the central cell and the sperm gives the nutritive endosperm. This fertilization process has been studied extensively for many years. However, despite these studies, relatively little is known at the molecular level about either of the plant gametes. This is primarily due to the difficulties in plant gamete isolation. Both plant gametes reside within enclosed tissues, pollen grains and embryo sacs. In this study, sperm isolation techniques were successfully developed for Brassica oleracea and Arabidopsis thaliana which ultimately utilised fluorescence-activated cell sorting (FACS) to obtain pure cell samples. Proteomic studies utilising two-dimensional protein electrophoresis and mass spectrometry (MS) were carried out on both semi-purified and FACS-purified sperm cells. In parallel with the proteomic studies a bioinformatics approach was taken which used sperm transcriptome data of maize, Plumbago zeylanica, rice and tobacco to identify homologues in Arabidopsis. Transcriptional analyses, RT-PCR and GFP translational fusion experiments were used to investigate these Arabidopsis sperm cell-expressed gene candidates. As a result, two sperm cell-expressed genes (At1g10090 and At5g39650) were identified and these are being analysed to confirm their functions in the reproductive process. Moreover, the sperm cell-expressed gene candidates derived from the bioinformatics were also screened for roles in plant reproduction by a reverse genetics approach (Arabidopsis T-DNA insertion mutagenesis plant screening) and eight genes were identified. In addition, for the first time, sperm cell size dimorphism was identified for Arabidopsis in this study utilising a GFP-labelled sperm line and confocal microscopy. Overall the techniques for sperm cell-expressed gene candidate selection were proven to be effective and will certainly facilitate further sperm cell-specific gene identification studies. Further the Arabidopsis sperm purification technique successfully developed in this project will surely be useful for any plant sperm cell studies in the future.
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Robinson, R., and Phillip R. Scheuerman. "Effect of Cadmium and Copper on Rabbit Sperm Cells." Digital Commons @ East Tennessee State University, 1996. https://dc.etsu.edu/etsu-works/2911.

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Blackwell, Shannen. "Forensic techniques for the isolation of sperm cells from mixed cell fractions: A review." Thesis, Blackwell, Shannen (2017) Forensic techniques for the isolation of sperm cells from mixed cell fractions: A review. Masters by Coursework thesis, Murdoch University, 2017. https://researchrepository.murdoch.edu.au/id/eprint/39830/.

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The process of separating one specific cell type from a mixed cellular sample has been utilised extensively by the scientific community. In particular, separating sperm cells from mixed cell samples, containing both male and female DNA, is typically employed within the forensic sciences. This review will examine the different sperm cell DNA isolation techniques which are used by forensic analysts, with a particular focus on differential extraction and Y-chromosome targeted DNA profiling, as these are currently the most widely used techniques. Throughout the literature, many modifications have been made to the original methods which were proposed, in an effort to increase the scientific value of both of these methods. Any method modifications which have been introduced will be reviewed, and their benefit for the forensic community will also be discussed. Although these methods have their uses, it is discovered by this review that they are both limited in their applicability, and by their overall success rates. For this reason, a more recently published technique of using immunomagnetic beads (IMBs) to separate the DNA fractions is proposed as an alternative method to the separation techniques currently employed by forensic DNA laboratories.
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Torgerson, Dara G. Singh R. S. "The molecular evolution of genes expressed in the sperm /." *McMaster only, 2004.

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Tramontozzi, Peter J. "Microtubule Dynamics During Sperm Aster Centration in Fertilized Sea Urchin Cells." Thesis, Boston College, 2018. http://hdl.handle.net/2345/bc-ir:108018.

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Thesis advisor: David R. Burgess
Centration of the nucleus after fertilization is an essential step for setting-up cell division and proper embryonic development in many proliferating cells such as the sea urchin. The sperm aster must capture the female pronucleus for fusion as well as the nucleus becoming positioned at the center of the cell. Microtubules (MTs) are known to play a role in this centration but the exact mechanism remains unknown. This begins to investigate current models of nuclear centration and the role of various interactions. Three phases of migration were observed as the male aster migrated with support in independent movements of the male and female pronuclei. Dimpling affects present that altered the morphology of the cell were observed when engagement occurred between the male and female pronuclei. It was discovered that this dimpling effect was a result of an interaction between MTs and the cortex, as confirmed by visualization of sheared cells in which only the cortex remained. Stemming from previous and current research in the lab, the role of post-translational modifications (PMTs) in nuclear centration was investigated for the different forces exerted due to various factors. Tyrosinated and detyrosinated populations were observed with and without the presence of parthenolide (PTL), an agent that inhibits detyrosination. PTL was observed to not only prevent the proper migration, but also that it expanded tyrosination of tubulin – which would further disrupt the force vectors created through the PMTs promotion of dyneins and kinesins. The results have lead to a new hypothesis to be furthered in order to gain an in-depth understanding in the mechanism(s) for pronuclear migration
Thesis (BS) — Boston College, 2018
Submitted to: Boston College. College of Arts and Sciences
Discipline: Departmental Honors
Discipline: Biology
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姚元慶 and Yuanqing Yao. "The effects of human oviductal cells and follicular fluid on sperm functions." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31239614.

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Books on the topic "Sperm cells"

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J, Wilms H., and Keijzer C. J. 1950-, eds. Plant sperm cells as tools for biotechnology. Wageningen: Pudoc, 1988.

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Smallwood, Alan Victor. Chromatin structure of the imprinted H19 and IGF2 genes in human sperm nuclei and cultured cells. Birmingham: University of Birmingham, 2000.

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Alan, Trounson, and Wood Carl, eds. Atlas of fine structure of human sperm penetration, eggs, and embryos cultured in vitro. New York: Praeger, 1985.

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Yanagimachi, Ryuzo. The Sperm Cell. Edited by Christopher J. De Jonge and Christopher L. R. Barratt. Cambridge: Cambridge University Press, 2017. http://dx.doi.org/10.1017/9781316411124.

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De Jonge, Christopher J., and Christopher Barratt, eds. The Sperm Cell. Cambridge: Cambridge University Press, 2006. http://dx.doi.org/10.1017/cbo9780511545115.

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1930-, Mohri Hideo, ed. New horizons in sperm cell research. Tokyo: Japan Scientific Societies Press, 1987.

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1929-, Sinowatz Fred, ed. Cytochemical analysis of mammalian sperm membranes. Stuttgart: Fischer, 1989.

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De, Jonge Christopher J., and Barratt C. L. R, eds. The sperm cell: Production, maturation, fertilization, regeneration. Cambridge, UK: Cambridge University Press, 2006.

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Contraceptive Research and Development Program. International Workshop. Gamete interaction: Prospects for immunocontraception : proceedings of the Third Contraceptive Research and Development (CONRAD) Program International Workshop, cosponsored by the World Health Organization, held in San Carlos de Bariloche, Argentina, November 26-29, 1989. Edited by Alexander Nancy J, Contraceptive Research and Development Program., and World Health Health Organization. New York: Wiley-Liss, 1990.

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Thomas, Lejeune, and Delvaux Pascal, eds. Human spermatozoa: Maturation, capacitation, and abnormalities. Hauppauge, N.Y: Nova Science Publishers, 2009.

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Book chapters on the topic "Sperm cells"

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Saucedo, Leslie. "Sperm." In Getting to Know Your Cells, 67–71. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-30146-9_12.

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Rauch, Anita. "Human Sperm Cells." In FISH Technology, 127–37. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56404-8_9.

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Lee, Tin-Lap, Albert Hoi-Hung Cheung, Owen M. Rennert, and Wai-Yee Chan. "RNA Expression in Male Germ Cells During Spermatogenesis (Male Germ Cell Transcriptome)." In Sperm Chromatin, 107–21. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9_8.

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Dai, Changsheng, and Yu Sun. "Robotic Sperm Immobilization." In Robotic Manipulation of Reproductive Cells, 21–39. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-52730-2_3.

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Dai, Changsheng, and Yu Sun. "Automated Sperm Analysis." In Robotic Manipulation of Reproductive Cells, 7–20. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-52730-2_2.

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Weber, M. "Sperm Cells in Apiaceae." In Sexual Reproduction in Higher Plants, 491. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73271-3_103.

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Silber, Sherman. "Making Sperm from Skin Cells." In Fundamentals of Male Infertility, 187–92. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-76523-5_19.

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Dai, Changsheng, and Yu Sun. "Automated Picoliter-Resolution Sperm Aspiration." In Robotic Manipulation of Reproductive Cells, 41–51. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-52730-2_4.

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Lee, Tin-Lap, Albert Hoi-Hung Cheung, Owen M. Rennert, and Wai-Yee Chan. "RNA Expression in Male Germ Cells During Spermatogenesis (Male Germ Cell Transcriptome)." In Sperm Chromatin for the Researcher, 105–23. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-8459-2_7.

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Weyand, I. "Cyclic Nucleotide-Gated Channels in Sperm." In Signal Transduction in Testicular Cells, 53–68. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-662-03230-5_4.

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Conference papers on the topic "Sperm cells"

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Butola, Ankit, Sigurd Hellberg, Mona Nystad, and Krishna Agarwal. "Quantitative phase imaging and machine learning for spermatozoa analysis." In 3D Image Acquisition and Display: Technology, Perception and Applications, JM4A.6. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/3d.2024.jm4a.6.

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Conventional methods of assessing sperm quality are based on qualitative analysis of semen. In this paper, we combined time-lapse quantitative phase imaging and deep learning for the classification of healthy and unhealthy sperm cells.
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Jankowski, Marcin, Emila Lewandowska, Hugues Talbot, Daniel Wesierski, and Anna Jezierska. "Learning sperm cells part segmentation with class-specific data augmentation." In 2024 16th International Conference on Human System Interaction (HSI), 1–6. IEEE, 2024. http://dx.doi.org/10.1109/hsi61632.2024.10613572.

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Hamidu, Aisha, Ahmed Azmeer, Omar Abdelgawad, Megan Ghaly, and Mohamed Abdelgawad. "A Microfluidic Platform for Analysis of Beating Characteristics of Sperm Cells." In 2024 IEEE 19th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS), 1–4. IEEE, 2024. http://dx.doi.org/10.1109/nems60219.2024.10639857.

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Park, Daniel S., Robert Egnatchik, Hali Bordelon, Terrence R. Tiersch, and W. Todd Monroe. "A Microfluidic Mixer to Activate Sperm Cells of Aquatic Species for Standardization of Computer-Assisted Motion Analysis." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53839.

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The objective of this paper is to develop a microfluidic device to: 1) activate a small volume of aquatic species sperm by rapid mixing with diluent, and 2) position sperm in a viewing chamber for motility evaluation using computer-assisted sperm analysis (CASA). Analysis of aquatic species sperm is becoming more important as the use of fish as biomedical models expands. Because it is more efficient to maintain frozen stocks of genetic material rather than thousands of research lines of adult fish, there has been increased study on cryopreservation for model fish. The analysis of fish gametes is challenging due to small sample size, short motility duration, and inconsistent activation (motility induction). For many aquatic species, sperm motility is initiated through the manual alteration of the medium osmolality, typically accomplished through manual dilution and mixing by hand. Manual methods limit control over the activation process and therefore viability analysis. The short lifespan of these cells makes CASA challenging due to the limitations in capturing and processing data rapidly enough to monitor the peak motility, as CASA systems were designed for mammalian sperm which have a longer motility duration.
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V.P., Osipova, and Kolyada M.N. "APPLICATION OF NEW GENERATION ANTIOXIDANTS AS CRYOPROTECTORS IN LOWTEMPERATURE PRESERVATION OF STURGEON REPRODUCTIVE CELLS." In II INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE "DEVELOPMENT AND MODERN PROBLEMS OF AQUACULTURE" ("AQUACULTURE 2022" CONFERENCE). DSTU-Print, 2022. http://dx.doi.org/10.23947/aquaculture.2022.103-105.

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The paper presents the results of a study of the cryoprotective activity of a new generation of antioxidants – hybrid multifunctional phenolic derivatives during low-temperature preservation of sturgeon reproductive cells. It has been shown that the addition of phosphorus-containing sterically hindered phenol, pyrrolidine and thioacetamide phenolic derivatives to the basic modified Stein cryoprotective medium, makes it possible to increase the low cryoresistance of sturgeon sperm: the level of lipid peroxidation decreases, cell motility improves, and the fertility of defrosted sperm increases. The effectiveness of the cryoprotective action of new antioxidants that can affect different stages and links of oxidative cryostress exceeds the activity of known antioxidants (Ionol and Trolox).
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Liu, Yagang, Gregory J. Sonek, Curtis F. Chapman, Bruce J. Tromberg, Pasquale Patrizio, Yona Tadir, and Michael W. Berns. "Microthermometry of laser-heated Chinese hamster ovary cells and sperm cells." In Photonics West '95, edited by Steven L. Jacques. SPIE, 1995. http://dx.doi.org/10.1117/12.209917.

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Chen, Hsiu-hung Simon, Zhiquan Shu, Lei Cheng, and Dayong Gao. "Development of a Microfluidic Injection and Perfusion Device for Single Cell Study." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13317.

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The cell membrane, composed primarily of proteins and lipids, is a selectively permeable lipid bilayer in the scale of 10 nm or so. Molecules permeating through cell membranes play critical roles in the applications of drug delivery, cell therapy, and cryopreservation. Cryopreservation and banking of cells, such as umbilical cord bloods, female eggs, etc., are critical to facilitate practical and effective in vitro fertilization (IVF). The determination of molecule transport properties of cells, such as water and cryoprotectants (CPAs), is indispensable for developing optimal conditions for cryopreserving them. On the other hand, injection of material of interests, such as sperms and DNA segments, to female eggs or blastocysts, so-called intracytoplasmic sperm injection (ICSI) technique, are playing important roles on IVF and advanced gene knock-out. In this study, a novel micro-nano-fluidic system that allows perfusion and injection in nano-liter scale has been developed and fabricated by soft lithographic methods. A single cell in the microfluidic system is able to be trapped on site and then either be perfused by various solutions or injected with plain solutions or solutions with genetic materials. Our ongoing study will demonstrate that the micro-nano-fluidic system allows us to: 1) confine cells in a channel; 2) deliver drugs by perfusing the cell; 3) monitor osmotic behaviors of the cell by replacing its extracellular environment; and 4) perform ICSI with sperms or genetic materials.
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Bhowmick, Sankha, Bharat D. Nath, John D. Biggers, and Mehmet Toner. "Thermostability Studies of Desiccated Murine Spermatozoa Nuclear DNA to Predict the Beneficial Effect of Trehalose in Long Term Storage." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-33684.

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Long term preservation of mouse sperm in a desiccated state using sugars like trehalose may offer attractive economic benefits in the management of rapidly increasing transgenic mouse strains. The goal of the current study was to evaluate the protective effect of intracellular trehalose on sperm nucleus by predicting the long-term nuclear degradation kinetics of desiccated spermatozoa using an Arrhenius model whose parameters are obtained from high temperature-short time storage studies. B6D2F1 sperm isolated in an EGTA supplemented tris-HCl buffer (with or without 0.5M intracellular trehalose) were convectively dried with inert nitrogen gas in a controlled manner to moisture content &gt;5%. The samples were then vacuum packed and stored at 22, 37, 45, 60 and 90°C for 1, 3 or 7 days. Following rehydration, the sperm sample was assayed for DNA damage using the sperm chromatin structure assay (SCSA). Results indicate significantly (p&gt;0.05) lower DNA degradation for cells dried with intracellular trehalose at 45, 60 and 90°C for 1, 3 or 7 days compared to cells dried without trehalose. Based on a 10% increase in the index of injury, the calculated activation energy and frequency factors were 10.33 kcal/mole and 5.4×105 hr−1 respectively for cells dried in EGTA solution only. The corresponding numbers for cells dried in EGTA solution supplemented with 0.5M trehalose were 5.7 kcal/mole and 43.73 hr−1. Based on these parameters the time required for 10% DNA degradation are 279 and 759 hours for samples desiccated in plain EGTA vs. trehalose supplemented EGTA. These results indicate the beneficial effect of intracellular trehalose for the long-term storage of desiccated sperm.
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Scherr, Thomas F., Gerald Knapp, Terrence Tiersch, W. Todd Monroe, and Krishnaswamy Nandakumar. "The Activation of Zebrafish Sperm Cells in a Micromixer." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14734.

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The freshwater fish, Danio rerio (zebrafish), have become widely used as a model organism for vertebrate development, DNA mutation, and human disease studies [1]. Maintaining live colonies of the numerous developed strains of zebrafish under investigation can be prohibitively costly. As such, there is a growing need to catalog their reproductive cells and have them available on demand [2]. Thus cryopreservation of model strain gametes has become an important endeavor, where evaluation of freezing and thawing techniques is currently a bottleneck to these procedures.
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Ferrara, M. A., A. De Angelis, G. Coppola, A. C. De Luca, and G. Coppola. "Polarization digital holography for birefringence characterization of sperm cells." In 20th Italian National Conference on Photonic Technologies (Fotonica 2018). Institution of Engineering and Technology, 2018. http://dx.doi.org/10.1049/cp.2018.1663.

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Reports on the topic "Sperm cells"

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Prince, R. M. Sperm cells as vectors in the production of transgenic animals. Office of Scientific and Technical Information (OSTI), April 1993. http://dx.doi.org/10.2172/10175384.

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Young, Ronald J., and William C. Starke. A Procedure for the Removal of Vesicles and Prostate Secretions from Motile Rabbit Sperm Cells. Fort Belvoir, VA: Defense Technical Information Center, August 1988. http://dx.doi.org/10.21236/ada200288.

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Border, Peter, and Amarpreet Kaur. Human germline genome editing. Parliamentary Office of Science and Technology, January 2020. http://dx.doi.org/10.58248/pn611.

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Changes made to DNA in human eggs, sperm or embryos (germline cells) can be passed on to future generation. The methods used to to make such changes are referred to as human germline genome editing (hGGE). This POSTnote reviews techniques available for hGGE, their safety and potential applications. It also outlines current regulation and governance of hGGE and examines issues raised by any potential future uses of hGGE.
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Hansen, Peter J., and Zvi Roth. Use of Oocyte and Embryo Survival Factors to Enhance Fertility of Heat-stressed Dairy Cattle. United States Department of Agriculture, August 2011. http://dx.doi.org/10.32747/2011.7697105.bard.

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The overall goal was to identify survival factors that can improve pregnancy success following insemination or embryo transfer in lactating dairy cows exposed to heat stress. First, we demonstrated that oocytes are actually damaged by elevated temperature in the summer. Then we tested two thermoprotective molecules for their effect on oocyte damage caused by heat shock. One molecule, ceramide was not thermoprptective. Another, insulin-like growth factor-1 (IGF) reduced the effects of heat shock on oocyte apoptosis and oocyte cleavage when added during maturation. We also used lactating cows exposed to heat stress to determine whether bovine somatotropin (bST), which increases IGF1 levels in vivo, would improve fertility in summer. Cows treated with bST received a single injection at 3 days before insemination. Controls received no additional treatment. Treatment with bST did not significantly increase the proportion of inseminated cows diagnosed pregnant although it was numerically greater for the bST group (24.2% vs 17.8%, 124–132 cows per group). There was a tendency (p =0.10) for a smaller percent of control cows to have high plasma progesterone concentrations (≥ 1 ng/ml) at Day 7 after insemination than for bST-treated cows (72.6 vs 81.1%). When only cows that were successfully synchronized were considered, the magnitude of the absolute difference in the percentage of inseminated cows that were diagnosed pregnant between bST and control cows was reduced (24.8 vs 22.4% pregnant for bST and control). Results failed to indicate a beneficial effect of bST treatment on fertility of lactating dairy cows. In another experiment, we found a tendency for addition of IGF1 to embryo culture medium to improve embryonic survival after embryo transfer when the experiment was done during heat stress but not when the experiment was done in the absence of heat stress. Another molecule tested, granulocyte-macrophage colony-stimulating factor (GM-CSF; also called colony-stimulating factor-2), improved embryonic survival in the absence of heat stress. We also examined whether heat shock affects the sperm cell. There was no effect of heat shock on sperm apoptosis (programmed cell death) or on sperm fertilizing ability. Therefore, effects of heat shock on sperm function after ejaculation if minimal. However, there were seasonal changes in sperm characteristics that indicates that some of the decrease in dairy cow fertility during the summer in Israel is due to using semen of inferior quality. Semen was collected from five representative bulls throughout the summer (August and September) and winter (December and January). There were seasonal differences in ion concentration in seminal plasma and in the mRNA for various ion channels known to be involved in acrosome reactions. Furthermore, the proportion of sperm cells with damaged acrosomes was higher in post-thaw semen collected in the summer than in its counterpart collected in winter (54.2 ± 3.5% vs. 51.4 ± 1.9%, respectively; P < 0.08Further examination is required to determine whether such alterations are involved in the low summer fertility of dairy cows.
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Bodt, B. A., and R. J. Young. Hyperactivated Rabbit Sperm Cell Motility Parameters. Fort Belvoir, VA: Defense Technical Information Center, March 1995. http://dx.doi.org/10.21236/ada294502.

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Young, Ronald J., and David Burnett. Chemical Inhibition of Rabbit Sperm Cell Motility in Toxicological Testing. Fort Belvoir, VA: Defense Technical Information Center, January 1990. http://dx.doi.org/10.21236/ada219488.

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Ohad, Nir, and Robert Fischer. Control of Fertilization-Independent Development by the FIE1 Gene. United States Department of Agriculture, August 2000. http://dx.doi.org/10.32747/2000.7575290.bard.

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A fundamental problem in biology is to understand how fertilization initiates reproductive development. During plant reproduction, one sperm cell fuses with the egg to form an embryo, whereas a second sperm cell fuses with the adjacent central cell nucleus to form the endosperm tissue that supports embryo and/or seedling development. To understand the mechanisms that initiate reproduction, we have isolated mutants of Arabidopsis that allow for replication of the central cell and subsequent endosperm development without fertilization. In this project we have cloned the MEA gene and showed that it encode a SET- domain polycomb protein. Such proteins are known to form chromatin-protein complexes that repress homeotic gene transcription and influence cell proliferation from Drosophylla to mammals. We propose a model whereby MEA and an additional polycomb protein we have cloned, FIE , function to suppress a critical aspect of early plant reproduction and endosperm development, until fertilization occurs. Using a molecular approach we were able to determine that FIE and MEA interact physically, suggesting that these proteins have been conserved also during the evolution of flowering plants. The analysis of MEA expression pattern revealed that it is an imprinted gene that displays parent-of- origin-dependent monoallelic expression specifically in the endosperm tissue. Silencing of the paternal MEA allele in the endosperm and the phenotype of mutant mea seeds support the parental conflict theory for the evolution of imprinting in plants and mammals. These results contribute new information on the initiation of endosperm development and provide a unique entry point to study asexual reproduction and apomixis which is expected to improve crop production.
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Heifetz, Yael, and Michael Bender. Success and failure in insect fertilization and reproduction - the role of the female accessory glands. United States Department of Agriculture, December 2006. http://dx.doi.org/10.32747/2006.7695586.bard.

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The research problem. Understanding of insect reproduction has been critical to the design of insect pest control strategies including disruptions of mate-finding, courtship and sperm transfer by male insects. It is well known that males transfer proteins to females during mating that profoundly affect female reproductive physiology, but little is known about the molecular basis of female mating response and no attempts have yet been made to interfere with female post-mating responses that directly bear on the efficacy of fertilization. The female reproductive tract provides a crucial environment for the events of fertilization yet thus far those events and the role of the female tract in influencing them are poorly understood. For this project, we have chosen to focus on the lower reproductive tract because it is the site of two processes critical to reproduction: sperm management (storage, maintenance, and release from storage) and fertilization. E,fforts during this project period centered on the elucidation of mating responses in the female lower reproductive tract The central goals of this project were: 1. To identify mating-responsive genes in the female lower reproductive tract using DNA microarray technology. 2. In parallel, to identify mating-responsive genes in these tissues using proteomic assays (2D gels and LC-MS/MS techniques). 3. To integrate proteomic and genomic analyses of reproductive tract gene expression to identify significant genes for functional analysis. Our main achievements were: 1. Identification of mating-responsive genes in the female lower reproductive tract. We identified 539 mating-responsive genes using genomic and proteomic approaches. This analysis revealed a shift from gene silencing to gene activation soon after mating and a peak in differential gene expression at 6 hours post-mating. In addition, comparison of the two datasets revealed an expression pattern consistent with the model that important reproductive proteins are pre-programmed for synthesis prior to mating. This work was published in Mack et al. (2006). Validation experiments using real-time PCR techniques suggest that microarray assays provide a conservativestimate of the true transcriptional activity in reproductive tissues. 2.lntegration of proteomics and genomics data sets. We compared the expression profiles from DNA microarray data with the proteins identified in our proteomic experiments. Although comparing the two data sets poses analyical challenges, it provides a more complete view of gene expression as well as insights into how specific genes may be regulated. This work was published in Mack et al. (2006). 3. Development of primary reproductive tract cell cultures. We developed primary cell cultures of dispersed reproductive tract cell types and determined conditions for organ culture of the entire reproductive tract. This work will allow us to rapidly screen mating-responsive genes for a variety of reproductive-tract specifi c functions. Scientific and agricultural significance. Together, these studies have defined the genetic response to mating in a part of the female reproductive tract that is critical for successful fertllization and have identified alarge set of mating-responsive genes. This work is the first to combine both genomic and proteomic approaches in determining female mating response in these tissues and has provided important insights into insect reproductive behavior.
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