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1

Friedrich, Benjamin. "Chemotaxis of Sperm Cells." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1235056439247-79608.

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Sperm cells are guided to the egg by chemoattractants in many species. Sperm cells are propelled in a liquid by the regular beat of their flagellum. In the presence of a concentration gradient of a chemoattractant, they can steer upwards the concentration gradient, a process called chemotaxis. Eggs release chemoattractants to guide the sperm cells to the egg. Sperm chemotaxis is best studied experimentally in the sea urchin. There, specific receptors in the flagellar membrane of the sperm cells are activated upon binding of chemoattractant molecules and trigger a signaling cascade which ultima
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2

Friedrich, Benjamin. "Chemotaxis of Sperm Cells." Doctoral thesis, Technische Universität Dresden, 2008. https://tud.qucosa.de/id/qucosa%3A23708.

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Sperm cells are guided to the egg by chemoattractants in many species. Sperm cells are propelled in a liquid by the regular beat of their flagellum. In the presence of a concentration gradient of a chemoattractant, they can steer upwards the concentration gradient, a process called chemotaxis. Eggs release chemoattractants to guide the sperm cells to the egg. Sperm chemotaxis is best studied experimentally in the sea urchin. There, specific receptors in the flagellar membrane of the sperm cells are activated upon binding of chemoattractant molecules and trigger a signaling cascade which ultima
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3

Tam, Wing-hei Winky. "Adrenomedullin in oviduct and sperm function." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B39430248.

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4

Tam, Wing-hei Winky, and 譚詠曦. "Adrenomedullin in oviduct and sperm function." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39430248.

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5

Sriboonlert, Ajaraporn. "Proteome and transcriptional analysis of Arabidopsis thaliana sperm cells." Thesis, University of Bath, 2008. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538168.

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Angiosperm sexual reproduction is unique and remarkable. Unlike for other living organisms, fertilisation involves the fusion of two sperm cells to two cell types of female gametophyte, the egg and central cell. This fertilization process is called double fertilisation. Fusion of the egg and the sperm yields a zygote whereas the interaction of the central cell and the sperm gives the nutritive endosperm. This fertilization process has been studied extensively for many years. However, despite these studies, relatively little is known at the molecular level about either of the plant gametes. Thi
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6

Robinson, R., and Phillip R. Scheuerman. "Effect of Cadmium and Copper on Rabbit Sperm Cells." Digital Commons @ East Tennessee State University, 1996. https://dc.etsu.edu/etsu-works/2911.

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7

Blackwell, Shannen. "Forensic techniques for the isolation of sperm cells from mixed cell fractions: A review." Thesis, Blackwell, Shannen (2017) Forensic techniques for the isolation of sperm cells from mixed cell fractions: A review. Masters by Coursework thesis, Murdoch University, 2017. https://researchrepository.murdoch.edu.au/id/eprint/39830/.

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The process of separating one specific cell type from a mixed cellular sample has been utilised extensively by the scientific community. In particular, separating sperm cells from mixed cell samples, containing both male and female DNA, is typically employed within the forensic sciences. This review will examine the different sperm cell DNA isolation techniques which are used by forensic analysts, with a particular focus on differential extraction and Y-chromosome targeted DNA profiling, as these are currently the most widely used techniques. Throughout the literature, many modifications have
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8

Torgerson, Dara G. Singh R. S. "The molecular evolution of genes expressed in the sperm /." *McMaster only, 2004.

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9

Tramontozzi, Peter J. "Microtubule Dynamics During Sperm Aster Centration in Fertilized Sea Urchin Cells." Thesis, Boston College, 2018. http://hdl.handle.net/2345/bc-ir:108018.

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Thesis advisor: David R. Burgess<br>Centration of the nucleus after fertilization is an essential step for setting-up cell division and proper embryonic development in many proliferating cells such as the sea urchin. The sperm aster must capture the female pronucleus for fusion as well as the nucleus becoming positioned at the center of the cell. Microtubules (MTs) are known to play a role in this centration but the exact mechanism remains unknown. This begins to investigate current models of nuclear centration and the role of various interactions. Three phases of migration were observed as th
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10

姚元慶 and Yuanqing Yao. "The effects of human oviductal cells and follicular fluid on sperm functions." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31239614.

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11

Gaines, Jasmine P. "The role of sperm protein 17 (Sp17) in somatic cells and cancer." Birmingham, Ala. : University of Alabama at Birmingham, 2006. https://www.mhsl.uab.edu/dt/2008r/gaines.pdf.

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Thesis (Ph. D.)--University of Alabama at Birmingham, 2006.<br>Additional advisors: Vithal K. Ghanta, Denise R. Shaw, Stephen A. Watts, Bradley K. Yoder. Description based on contents viewed Feb. 20, 2009; title from PDF t.p. Includes bibliographical references.
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12

Yao, Yuanqing. "The effects of human oviductal cells and follicular fluid on sperm functions /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B20868212.

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13

Connolly, Thomas James. "Calcium signalling during human sperm interaction with cells of the female reproductive tract." Thesis, University of Birmingham, 2012. http://etheses.bham.ac.uk//id/eprint/3411/.

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The female reproductive tract acts not only as a complex mediator of sperm function and selection but animal data suggests that it alters protein expression after exposure to sperm, implying two-way communication. We have used single-cell fluorescence imaging to observe [Ca2+]i signalling in human female reproductive tract cells upon initial contact with sperm and in sperm during binding and release events. Parallel experiments were also performed on a model human oviductal cell line, OE E6/E7 and a control, human foreskin fibroblasts. Upon exposure to sperm, tract cells generated [Ca2+]i sign
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14

Smallwood, Alan Victor. "Chromatin structure of imprinted H19 and IGF2 genes in human sperm nuclei and cultured cells." Thesis, University of Birmingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393837.

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15

Hutchinson, Sean. "Utilising antigen-antibody complexes to capture and enrich for sperm cells fractions in a mixed substrate." Thesis, Hutchinson, Sean (2017) Utilising antigen-antibody complexes to capture and enrich for sperm cells fractions in a mixed substrate. Masters by Coursework thesis, Murdoch University, 2017. https://researchrepository.murdoch.edu.au/id/eprint/38406/.

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Forensic DNA analysis on sexual assault evidence requires the separation of the victim and assailants DNA. For vaginal swabs where a large number of victims cells (epithelial cells) are present compared to the small number of the male assailants cells this can be problematic. There are a variety of methods that can achieve this separation and the most commonly used at present is differential lysis or variations upon this method. These methods tend to be long and tedious with results often relying on the operators’ expertise and experience. Immunomagnetic beads have been used in various other a
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16

Taimur, Nadia. "A study of molecular and genetic mechanisms mediating the formation of twin sperm cells in Arabidopsis thaliana." Thesis, University of Leicester, 2014. http://hdl.handle.net/2381/37237.

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In flowering plants, the male gametophyte produces a pair of functional sperm cells that are transported to the embryo sac for double fertilisation. Asymmetric division of the microspore establishes the germline and division of the germ cell results in two sperm cells, however, the molecular mechanisms governing the germ cell specification and division are yet to be uncovered. DUO1 has been identified as a germline-specific MYB binding transcription factor that coordinates germ cell division with gamete specification. One of the major objectives of this thesis is the characterization of an EMS
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17

Yeste, Oliveras Marc. "New insights into boar sperm function and survival from integrated field and laboratory studies." Doctoral thesis, Universitat de Girona, 2008. http://hdl.handle.net/10803/7633.

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En aquesta tesi s'han dut a terme dos tipus d'estudis diferents. L'objectiu del primer era la preservació del semen de porcí a 15ºC i el del segon eren els co-cultius homòlegs de cèl·lules epitelials de l'oviducte i espermatozoides de porcí. <br/>Pel que fa al primer estudi, s'ha observat que l'addició de la prostaglandina F2&#945; i àcid hialurònic a les dosis seminals no malmena la qualitat espermàtica i que la tolerància dels espermatozoides als canvis d'osmolalitat del medi es pot correlacionar proves de fertilitat i prolificitat..<br/>Respecte el segon, s'ha determinat que les cèl·lules o
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Lassen, Paula Graziela. "Morfometria e desenvolvimento das gônadas de tilápias (Oreochromis niloticus) suplementadas com sal mineral composto por cobre, manganês e zinco." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/143757.

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O objetivo deste trabalho foi o de avaliar o efeito da suplementação da dieta de Tilápias (Oreochromis niloticus) utilizando sal comercial composto dos microminerais cobre, manganês e zinco, sobre a histologia e o desenvolvimento das gônadas dos machos. Foram utilizados 1200 machos masculinizados de tilápia, com média de peso de 120g, suplementadas com níveis crescentes (0,00; 0,35; 0,70; 1,05; 1,40 e 1,75 mg/kg) de um produto comercial composto por Cu 13,40 g/kg; Mn 26,70 g/kg e Zn 66,70 g/kg. O experimento foi conduzido em um sistema de recirculação de água, composto por 24 tanques divididos
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19

Kopeika, Julia. "The effect of cryopreservation on the genome of fish reproductive cells." Thesis, University of Bedfordshire, 2003. http://hdl.handle.net/10547/558306.

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Cryopreservation has been extensively used in human reproductive medicine, aquaculture and conservation programs for endangered species. Many studies have been devoted to the mechanisms of cryodamage. However, in spite of growing successes of cryopreservation, post-thaw recovery of reproductive and embryonic cells often remains poor. It is known that cryopreservation causes extensive damage to membrane, results in decreased metabolism of cells, and disturbs the bioenergetical processes of cells by damage to mitochondria. Nonetheless, it has not yet been identified clearly if cryopreservation c
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20

Young, Karen Elizabeth. "Interactions of nickel (II) with human protamine 2 in sperm cells a mechanism of toxicity and implication for industrial exposure /." Diss., Restricted to subscribing institutions, 2009. http://proquest.umi.com/pqdweb?did=1835198971&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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21

Pipart, Perrine. "Biopolymères pour l'insémination artificielle." Electronic Thesis or Diss., Sorbonne université, 2024. http://www.theses.fr/2024SORUS217.

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Les biotechnologies de la reproduction par insémination artificielle sont largement répandues dans le secteur primaire de l'élevage, notamment pour des espèces telles que les bovins, les porcins, les équins et les aviaires. L'insémination artificielle permet de maîtriser la fertilité et d'améliorer la génétique des cheptels en s'affranchissant des contraintes liées au déplacement des animaux. Elle rend possible la fécondation d'un grand nombre de femelles avec la semence d'un seul mâle. Les descendants héritant d'une partie du patrimoine génétique du mâle, ce dernier est choisi en fonction de
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22

Osman, Ilham F. "Effect of nanoparticles on human cells from healthy individuals and patients with respiratory diseases." Thesis, University of Bradford, 2010. http://hdl.handle.net/10454/4864.

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Ever increasing applications of nanomaterials (materials with one or more dimension less than 100 nm) has raised awareness of their potential genotoxicity. They have unique physico-chemical properties and so could have unpredictable effects. Zinc oxide (ZnO) and titanium dioxide (TiO2) are widely used in a number of commercial products. There are published studies indicating that some forms of these compounds may be photo-clastogenic in mammalian cells. What has not been investigated before is the effect of nanoparticles from these compounds in human germ cells. Thus the present study has exam
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23

Carlsson, Lena. "Perspectives on the Biological Role of Human Prostasomes." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2001. http://publications.uu.se/theses/91-554-5159-4/.

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24

Taylor, Jacqueline Susan. "The Effect of Pyrethroid Compounds on the Expression of Estrogen Receptors in Mouse Sertoli Cells and Implications for Male Infertility." Thesis, University of Canterbury. Biological Sciences, 2006. http://hdl.handle.net/10092/1482.

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Male fertility is largely controlled by the hypothalamic-pituitary axis, a careful balance between stimulating and suppressing gene expression and the secretion of hormones. The critical factors for male fertility have in the past been thought to be limited to testosterone and the gonadotropins. Estrogen has only recently been demonstrated to be both a crucial requirement for fertility and a cause of infertility. Reports in the early 1990s demonstrated a decrease in mean sperm counts over the last 50 years. A hypothesis for this observation is the increase of xenoestrogens in the environment t
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Flores, Jonas Gomes. "Fatores relacionados à determinação do sexo de potros da raça PSC." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2018. http://hdl.handle.net/10183/179251.

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As biotécnicas da reprodução na espécie equina avançaram na última década e os criadores de equinos começaram a questionar as possibilidades de interferência na determinação do sexo dos potros. A determinação do sexo é importante, pois o sexo do potro possui grande influência no valor comercial do mesmo. O objetivo do presente estudo foi avaliar a influência do momento da cobertura em relação ao momento da ovulação no sexo dos potros e outros fatores como: idade da égua e garanhão; indutor da ovulação; ovário em que ocorreu a ovulação e o diâmetro do folículo pré-ovulatório também foram avalia
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26

Eckmann, Christian R., Mark Schmid, Adam P. Kupinski, Britta Jedamzik, Martin Harterink, and Agata Rybarska. "GLS-1, a novel P granule component, modulates a network of conserved RNA regulators to influence germ cell fate decisions." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-184095.

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Post-transcriptional regulatory mechanisms are widely used to influence cell fate decisions in germ cells, early embryos, and neurons. Many conserved cytoplasmic RNA regulatory proteins associate with each other and assemble on target mRNAs, forming ribonucleoprotein (RNP) complexes, to control the mRNAs translational output. How these RNA regulatory networks are orchestrated during development to regulate cell fate decisions remains elusive. We addressed this problem by focusing on Caenorhabditis elegans germline development, an exemplar of post-transcriptional control mechanisms. Here, we re
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Bryan, Emily Rose. "Chronic Chlamyida infections in males: Impacts on testicular function and spermatogenesis." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/119158/1/Emily_Bryan_Thesis.pdf.

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Approximately 131 million Chlamydia trachomatis infections occur annually. The rates of infection are similar between males and females, but female infections have been more widely characterised. C. trachomatis causes infertility in men and women but little is known about the male disease. This study investigated the ability of chronic infections to cause male infertility. In mice, Chlamydia colonises specialised testicular cells, resulting in partial destruction of the testicular structure and production of anti-sperm-antibodies. This correlates with decline in sperm health and the egg-bindin
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28

Bergmann, Annabel Elisabeth [Verfasser], Christoph [Akademischer Betreuer] Knorr, Detlef [Gutachter] Rath, and Wolfgang [Gutachter] Holtz. "Sperm binding properties to uterine epithelial cells in vitro employing a primary porcine endometrium culture system / Annabel Elisabeth Bergmann. Betreuer: Christoph Knorr. Gutachter: Detlef Rath ; Wolfgang Holtz." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2016. http://d-nb.info/1102535877/34.

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29

Eckmann, Christian R., Mark Schmid, Adam P. Kupinski, Britta Jedamzik, Martin Harterink, and Agata Rybarska. "GLS-1, a novel P granule component, modulates a network of conserved RNA regulators to influence germ cell fate decisions." PLOS Genetics, 2009. https://tud.qucosa.de/id/qucosa%3A28993.

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Post-transcriptional regulatory mechanisms are widely used to influence cell fate decisions in germ cells, early embryos, and neurons. Many conserved cytoplasmic RNA regulatory proteins associate with each other and assemble on target mRNAs, forming ribonucleoprotein (RNP) complexes, to control the mRNAs translational output. How these RNA regulatory networks are orchestrated during development to regulate cell fate decisions remains elusive. We addressed this problem by focusing on Caenorhabditis elegans germline development, an exemplar of post-transcriptional control mechanisms. Here, we re
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30

Martins, Soraia Alexandra Araújo. "CD81 interacting proteins." Master's thesis, Universidade de Aveiro, 2016. http://hdl.handle.net/10773/16139.

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Mestrado em Biomedicina Molecular<br>Fertilization is a multistep and complex process culminating in the merge of gamete membranes, cytoplasmic unity and fusion of genome. CD81 is a tetraspanin protein that participates in sperm-oocyte interaction, being present at the oocyte surface. CD81 has also been implicated in other biological processes, however its specific function and molecular mechanisms of action remain to be elucidated. The interaction between CD81 and its binding partner proteins may underlie the CD81 involvement in a variety of cellular processes and modulate CD81/interactors sp
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31

Ali, Aftab H. M. "Genotoxicity of haloacetic acids, aspirin and ibuprofen in human cells : genotoxic effects of water disinfectant by-products in human blood and sperm and bulk and nano forms of aspirin and ibuprofen in human blood of respiratory disease patients." Thesis, University of Bradford, 2014. http://hdl.handle.net/10454/14626.

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This project focuses on two important topics which may pose hazards to human health. Firstly, drinking water disinfection by-products (DBPs), which are generated by the chemical disinfection of water have been investigated. What has not been shown is the effect of DBPs in human germ cells as well as somatic cells and whether oxidative stress is involved in the mechanism of genotoxic action. Three different DBPs (halo acetic acids: HAAs), together with the antioxidants – catalase and butylated hydroxyanisole (BHA), were investigated in peripheral blood cells and sperm from healthy individuals u
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32

Steiner, Jessica Kathryne. "Characterization of sterility and germline defects caused by Smed-boule RNA-interference." Wright State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1463490878.

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33

Kongmanas, Kessiri. "Roles of Seminolipid and Its Associated Membrane Domain in Male Fertility." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32509.

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Our research aims at understanding the roles of seminolipid (sulfogalactosylglycerolipid or SGG) and its associated membrane domains in male reproduction. SGG is a sulfoglycolipid present selectively and abundantly in mammalian male germ cells. Therefore, information on its properties would be relevant towards the development of male fertility biomarkers and spermicide-based contraceptives. We have shown that SGG has direct affinity for zona pellucida (ZP, egg extracellular matrix) and plays a role in the formation of sperm lipid rafts, the ZP-binding platforms on the sperm anterior head pl
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34

Achikanu, Cosmas Ezekaibeya. "Regulation of sperm motility by cell-signalling events in human sperm." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7907/.

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Ca²⁺ signals from activated Ca²⁺ channels (CatSper) and mobilisation of Ca²⁺ stores regulate human sperm cell behaviour as they ascend the female tract. I investigated the effects on human sperm [Ca²⁺]i and behaviour of CatSper channel activation (alkaline pH and progesterone) and Ca²⁺-store mobilisation (4-aminopyridine, thimerosal) using a fluorescence plate reader and CASA. Extracellular alkalinisation raised pH¬i (pHi = 6.9 and 7.2 at pHo7.4 and 8.5 respectively), caused tonic elevation of [Ca²⁺]i, which was partially inhibited by CatSper block and increased the proportion of hyperactivate
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35

Huang, Wenxin, and 黃聞馨. "Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/196485.

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Oxidative damage by reactive oxygen species (ROS) is a major cause of sperm dysfunction. Excessive ROS generation reduces fertilization and enhances DNA damage of spermatozoa. In mammals, including humans, oviduct functions as a sperm reservoir which is created by the binding of spermatozoa to the epithelial lining in the oviduct. Interaction between sperm and oviductal epithelial cells improves the fertilizing ability of and reduces chromatin damage in spermatozoa. However, the mechanism(s) by which spermatozoa-oviduct interaction producing these beneficial effects is unknown. One possibility
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36

Gavin-Plagne, Lucie. "Cryoconservation de cellules spermatiques et de cellules souches pluripotentes de mammifères dans un milieu synthétique et chimiquement défini." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1197/document.

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Cette thèse s’inscrit dans le cadre du projet CRB-Anim (Centre de Ressources Biologiques d’Animaux Domestiques) dont le but est de constituer une cryobanque nationale et d’améliorer les techniques de cryoconservation. Aujourd’hui, les ressources biologiques de type reproductif (embryons, sperme, ovocytes) et de type somatique (fibroblastes et cellules souches pluripotentes) sont conservées dans des milieux contenant des produits d’origine animale (POA). L’utilisation actuelle des POA (sérums, lait, jaune d’œuf) pose des problématiques sanitaires (risque de contamination) et scientifiques (manq
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Sousa, Patrícia da Cunha. "Estudo dos danos morfofuncionais causados pela criopreservação no sêmen de tatu-peba,Euphractus sexcinctus Wagler, 1830." Universidade Federal Rural do Semi-Árido, 2013. http://bdtd.ufersa.edu.br:80/tede/handle/tede/344.

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Made available in DSpace on 2016-08-15T20:31:13Z (GMT). No. of bitstreams: 1 PatriciaCS_DISSERT.pdf: 1847560 bytes, checksum: 7da5854056af7ddc3546a8a90de2324f (MD5) Previous issue date: 2013-08-30<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The objective of this work, cryopreserve semen from six-banded armadillos, assessing in advance the effects of centrifugation and dilution of their seminal parameters over thermoresistance test (TRT) in order to define the best diluent to be used in semen cryopreservation of this species. Semen samples (n = 12) derived from 04 adult
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Stein, Kathryn Kreimborg. "Sperm proteins involved in sperm-egg fusion : a cell biological and proteomic approach /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.

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Kavak, Ants. "Evaluation of sperm production, testicular measurements and post-thaw sperm quality in Tori and Estonian breed stallions /." Uppsala : Dept. of Obstetrics and Gynaecology, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/9329559.pdf.

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Santiago, Joana Filipa Marques. "Modulation of sperm motility using cell-penetrating peptides." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22325.

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Mestrado em Biologia Molecular e Celular<br>The large number of unintended pregnancies worldwide due to the non-use or failure of contraceptive methods and the fact that male contraceptives are limited to condom and vasectomy, highlight the urgent need for the development of new contraceptive methods. The mechanism of sperm motility acquisition in the epididymis constitutes an ideal target for new pharmacological male contraceptives since only the post-testicular sperm maturation is affected. It is known that protein phosphatase 1 subunit gamma 2 (PPP1CC2), a PPP1 isoform only present i
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Englhart, Maria [Verfasser], and Stefanie [Akademischer Betreuer] Sprunck. "Direct gamete interactions and EGG CELL1-mediated sperm cell activation in Arabidopsis thaliana / Maria Englhart ; Betreuer: Stefanie Sprunck." Regensburg : Universitätsbibliothek Regensburg, 2018. http://d-nb.info/1173974644/34.

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42

Wasbrough, Elizabeth. "Comparative genomic and evolutionary analysis of sperm proteomes." Thesis, University of Bath, 2011. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538135.

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While the central role of spermatozoa in sexual reproduction and fertilization is well understood, many functional attributes of sperm have yet to be elucidated at the molecular level. One key to ultimately understand the molecular basis of sperm function is to comprehensively characterize its biochemical composition. This crucial information has been lacking, as molecular characterization of the sperm cell cannot be assessed by classic gene expression assays since mature spermatozoa are transcriptionaly inert. Whole-cell shotgun proteomic approaches have revolutionized the molecular analysis
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43

Abdulmwli, Mhamoued A. A. "Biomarkers of Genotoxic and Reprotoxic Effects after Chemical Exposure. The genotoxic effects due to the respiratory disease of Tuberculosis (TB) patients compared to healthy controls in diploid lymphocyte and haploid sperm cells, after treated with two heterocyclic amines and quercetin in bulk and nano forms." Thesis, University of Bradford, 2019. http://hdl.handle.net/10454/18335.

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In the tuberculosis patients, Mycobacterium tuberculosis can stimulate production of hydrogen peroxide in the host as a result of immune response. The H2O2 accumulate in pulmonary cells, causing oxidative stress that could lead to the cancer. We select TB patients for this study which investigates the effects of quercetin as there is an increased incidence of latent TB among the migrant population in the past few years and TB can increase the risk of cancer. Sperm and lymphocytes were treated with DNA damage inducers and quercetin (10µM, 25µM and 100µM), the responses evaluated usi
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44

Richmond, Alissa Gale. "Sperm-Oocyte Membrane Interactions during Fertilization in the Nematode Caenorhabditis elegans." W&M ScholarWorks, 2004. https://scholarworks.wm.edu/etd/1539624377.

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45

Seo, Duckbong. "A development of the motile sperm sorting microfluidic devices." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4798.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2007.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on December 13, 2007) Vita. Includes bibliographical references.
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Nance, Jeremy Franklin. "A molecular and genetic analysis of Caenorhabditis elegans sperm development." Diss., The University of Arizona, 1999. http://hdl.handle.net/10150/289079.

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As animals develop, cell differentiation is controlled through the actions of numerous signaling pathways. Typically, differentiation signals lead to the regulation of various transcription factors, which in turn regulate expression of sets of genes. In certain cells incapable of realizing new gene product expression, alternate strategies must be used to control development. The transition of a C. elegans spermatid (which has no ribosomes) to a spermatozoon is one such example of an atypical developmental event. How does the signaling pathway that controls this cell differentiation differ from
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47

Gil, Corbalán María Antonia. "Influencia de diferentes condiciones de cocultivo sobre la fecundación y la producción in vitro de embriones porcinos." Doctoral thesis, Universidad de Murcia, 2001. http://hdl.handle.net/10803/10979.

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Para reducir la incidencia de las penetraciones polispérmicas en los sistemas de fecundación in vitro porcina (FIV), los objetivos del presente trabajo fueron estudiar: 1) el efecto de tres volúmenes de medio de coincubación (2, 1 y 0'1 ml) y tres números de ovocitos (50, 30 y 15), inseminados con 6 x 105 espermatozoides /ml (primera experiencia) y 2000:1 espermatozoides:ovocito (segunda experiencia); 2) el efecto de la presencia de células del cumulus durante la FIV de ovocitos inseminados con diferentes ratios espermatozoides:ovocito (2000:1; 3000:1; 4000:1, 6000:1 y 8000:1), en 0'1 ml de me
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48

Khatab, Hoda A. T. "Molecular and genetic mechanisms regulating sperm cell development in Arabidopsis thaliana." Thesis, University of Leicester, 2012. http://hdl.handle.net/2381/10139.

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Sexual reproduction in flowering plants involves the production of male and female gametes crucial for successful fertilisation, yet molecular mechanisms in these processes are largely unknown. The goal of the research presented in this thesis is to understand more about these mechanisms. A novel pollen division mutant, duo pollen 4 (duo4), that fails to produce twin sperm cells was investigated. Genetic analyses revealed that duo4 acts post-meiotically and does not affect female gametophyte development. The majority of duo4 germ cells complete DNA replication and fail to enter mitosis, but mu
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Durbarry, Anjusha. "Genetic analysis of sperm cell formation in Arabidopsis thaliana L. Heynh." Thesis, University of Leicester, 2004. http://hdl.handle.net/2381/29845.

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The genetics and cell biology of a novel class of mutants that affect microgametogenesis in Arabidopsis was investigated. These mutants termed duo pollen (duo) specifically block generative cell division preventing the formation of sperm cells. Genetic analyses revealed that duo1-duo6 gametophytically and are male-specific. The duo mutants mapped to different chromosomal locations. Phenotypic characterisation revealed that duo1-duo3 share similar but distant nuclear morphologies. Analysis of pollen grains exhibiting mitotic figures showed that the undivided generative cell in duo1 entered poll
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Lamy, Julie. "Régulation du fluide tubaire et des intéractions spermatozoïdes-oviducte chez le bovin." Thesis, Tours, 2017. http://www.theses.fr/2017TOUR4049.

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Le stockage et la maturation finale des spermatozoïdes, la fécondation et le développement embryonnaire précoce se déroulent au sein de l’oviducte. Afin de mieux comprendre la régulation de ces événements, un pool de fluides tubaires issus d’une centaine de vaches a été analysé pour son contenu en hormones stéroïdiennes, en protéines et en métabolites par chromatographie couplée à la spectrométrie de masse et par spectroscopie RMN. D’importantes régulations du stéroïdome, du protéome et du métabolome du fluide tubaire ont été mises en évidence au cours du cycle estrien et en fonction du côté d
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