Relevant bibliographies by topics / Sperm DNA Extraction

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters

Academic literature on the topic 'Sperm DNA Extraction'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Sperm DNA Extraction"

1

Anvar, Zahra, Bahia Namavar-Jahromi, Samaneh Ebrahimi, and Behrouz Gharesi-Fard. "Genomic DNA Extraction from Sperm." Journal of Advanced Medical Sciences and Applied Technologies 1, no. 2 (2015): 120. http://dx.doi.org/10.18869/nrip.jamsat.1.2.120.

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Pichler, F. B., M. L. Dalebout, and C. S. Baker. "Nondestructive DNA extraction from sperm whale teeth and scrimshaw." Molecular Ecology Notes 1, no. 1-2 (2001): 106–9. http://dx.doi.org/10.1046/j.1471-8278.2001.00027.x.

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Tsukada, K., H. Asamura, M. Ota, K. Kobayashi, and H. Fukushima. "Sperm DNA extraction from mixed stains using the Differex™ System." International Congress Series 1288 (April 2006): 700–703. http://dx.doi.org/10.1016/j.ics.2005.12.059.

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Beránek, Martin, Igor Sirák, Milan Vošmik, Jiří Petera, Monika Drastíková, and Vladimír Palička. "Carrier molecules and extraction of circulating tumor DNA for next generation sequencing in colorectal cancer." Acta Medica (Hradec Kralove, Czech Republic) 59, no. 2 (2016): 54–58. http://dx.doi.org/10.14712/18059694.2016.54.

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The aims of the study were:i) to compare circulating tumor DNA (ctDNA) yields obtained by different manual extraction procedures,ii) to evaluate the addition of various carrier molecules into the plasma to improve ctDNA extraction recovery, andiii) to use next generation sequencing (NGS) technology to analyzeKRAS,BRAF, andNRASsomatic mutations in ctDNA from patients with metastatic colorectal cancer. Venous blood was obtained from patients who suffered from metastatic colorectal carcinoma. For plasma ctDNA extraction, the following carriers were tested: carrier RNA, polyadenylic acid, glycogen
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SIRONEN, A., P. UIMARI, and J. VILKKI. "Comparison of different DNA extraction methods from hair root follicles to genotype Finnish Landrace boars with the Illumina PorcineSNP60 BeadChip." Agricultural and Food Science 20, no. 2 (2008): 143. http://dx.doi.org/10.2137/145960611797215709.

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Recent developments in sequencing methods have enabled whole genome sequencing of several species and the available sequence information has allowed the development of high throughput genotyping chips. However, these genotyping methods require high quality DNA. The possibility to genotype samples based on DNA from non-invasive sources would permit retrospective genotyping of previously collected samples and also facilitate the analysis of large populations e.g. for genomic selection. In this study we have developed and evaluated different DNA preparation methods from porcine hair root follicle
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D. Vaghela, Bhaumik, Hiren S. Gajjar, and Jayesh C. Jalondhara. "Rapid and effective method for the extraction of the genomic dna from sperm." International Journal of Current Advanced Research 6, no. 5 (2017): 3613–15. http://dx.doi.org/10.24327/ijcar.2017.3615.0331.

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Serra, Olga, Raffaele Frazzi, Alessio Perotti, Lorenzo Barusi, and Annamaria Buschini. "Use of FTA® classic cards for epigenetic analysis of sperm DNA." BioTechniques 64, no. 2 (2018): 45–51. http://dx.doi.org/10.2144/btn-2017-0101.

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FTA® technologies provide the most reliable method for DNA extraction. Although FTA technologies have been widely used for genetic analysis, there is no literature on their use for epigenetic analysis yet. We present for the first time, a simple method for quantitative methylation assessment based on sperm cells stored on Whatman FTA classic cards. Specifically, elution of seminal DNA from FTA classic cards was successfully tested with an elution buffer and an incubation step in a thermocycler. The eluted DNA was bisulfite converted, amplified by PCR, and a region of interest was pyrosequenced
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Daigneault, Bradford W., Sandeep K. Rajput, and George W. Smith. "Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input." BioTechniques 68, no. 3 (2020): 155–58. http://dx.doi.org/10.2144/btn-2019-0121.

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We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and agriculture, including clinical diagnoses of infertility, the identification of single-nucleotide polymorphisms and aberrant methylation patterns that can impact fertility, lower embryo development and contribute to heritable disease. The methods described here provide a reliable, simplistic approac
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Carter, Kim L., Bruce C. Robertson, and Bart Kempenaers. "A differential DNA extraction method for sperm on the perivitelline membrane of avian eggs." Molecular Ecology 9, no. 12 (2000): 2149–50. http://dx.doi.org/10.1046/j.1365-294x.2000.01114.x.

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Motoe Katoh and Ruby Valdivia. "Procedure for sperm collection and DNA extraction from the ductus deferens and caudal epididymis." Mutation Research/Genetic Toxicology 341, no. 1 (1994): 26–28. http://dx.doi.org/10.1016/0165-1218(94)90021-3.

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Dissertations / Theses on the topic "Sperm DNA Extraction"

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Nori, Deepthi V. "A Novel Method for Rapid and Selective Extraction of Male DNA from Rape Kits using Alkaline Lysis and Pressure Cycling Technology (PCT)." FIU Digital Commons, 2014. http://digitalcommons.fiu.edu/etd/1505.

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There is an increasing demand for DNA analysis because of the sensitivity of the method and the ability to uniquely identify and distinguish individuals with a high degree of certainty. But this demand has led to huge backlogs in evidence lockers since the current DNA extraction protocols require long processing time. The DNA analysis procedure becomes more complicated when analyzing sexual assault casework samples where the evidence contains more than one contributor. Additional processing to separate different cell types in order to simplify the final data interpretation further contributes
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Martinez, Rachael Elizabeth. "A novel differential extraction technique utilizing multiple enzymes: developing separation of non-sperm and sperm fractions." Thesis, 2015. https://hdl.handle.net/2144/13994.

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Processing sexual assault samples is a difficult time consuming task for the forensic analyst. Samples tend to be a mixture of the victim’s epithelial cells and the male suspect’s sperm cells that need to be separated prior to extraction of Deoxyribonucleic Acid (DNA). Without separation of the two cell types, the DNA extract would result in an uninterpretable mixture. In 1985, Peter Gill and colleagues outlined a procedure known as preferential lysis that would aid in the separation of female and male cells from sexual assault samples. The basis of this procedure, commonly referred to as a
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Rai, Anooja. "Optimization and validation of a novel direct-lysis differential extraction procedure." Thesis, 2018. https://hdl.handle.net/2144/33006.

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Forensic analysis of DNA from sexual assault kits is a laborious process. These samples may be a mixture of sperm and male or female epithelial cells (E-cells). Generally, it is the sperm cells that are of greatest forensic value. Since its introduction in 1985 by Gill, Jefferys and Warrett, differential extraction has remained an essential pre-PCR extraction procedure adopted by most forensic laboratories for the preferential lysis of E-cells and isolation of sperm cells/male fraction prior to DNA profiling. The differential extraction procedure operates based on the packaging of DNA in the
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Cassis, Patricia Rose. "Effectiveness of a novel extraction method for semen: comparison using liquid samples and dried stains." Thesis, 2016. https://hdl.handle.net/2144/16762.

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Forensic analysis of deoxyribonucleic acid (DNA) collected from sexual assault evidence is a multi-step process that requires a great amount of time and resources. A large percentage of samples are mixtures containing DNA from a major female contributor and at least one minor male contributor. The amount of male DNA present is often much less than that of the female, making it difficult to achieve a full short-tandem repeat (STR) profile for identification purposes. The current method employed by many forensic laboratories to separate sperm DNA from non-sperm DNA is the differential extra
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Taveira, Caitlyn Nicole. "Optimizing cell elution conditions for a novel enzymatic DNA extraction technique for spermatozoa on cotton swabs." Thesis, 2015. https://hdl.handle.net/2144/13989.

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Fundamentals of forensic deoxyribonucleic acid (DNA) typing for sexual assault samples require the successful application of a differential extraction. Gynecological swabs containing vaginal epithelial cells and sperm cells are commonly encountered in forensic casework. A priority in sexual assault casework is the identity of the male contributor and in order to identify the male contributor, separation of the vaginal epithelial cells and sperm cells must be achieved. Two considerations when separating the different cell-types from a substrate are cellular elution and purity of the DNA frac
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de, Gannes Matthew K. "Rapid Method of Processing Sperm for Nucleic Acid Extraction in Clinical Research." 2014. https://scholarworks.umass.edu/masters_theses_2/9.

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Background: Sperm contain highly compact nuclei, inhibiting DNA extraction using traditional techniques. Current methods extracting sperm DNA involve lengthy lysis and no means of stabilizing DNA, hindering clinical research. Objective: We sought to optimize an efficient method of extracting high quality human sperm DNA. Methods: Sperm from three volunteers were isolated using PureCeption. We tested 1) proteinase K with DNA/RNA Shield, 2) DTT and TCEP as reducing agents, 3) QIAshredder homogenization, and 4) stability of sperm DNA fresh (baseline) or after 4 weeks of storage at 4OC in DNA/RNA
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Stahlberger, Michele Elizabeth. "Optimization of sperm DNA extraction utilizing a multi-enzyme technique and preliminary experiments for the development of a novel fluorescent stain for sperm nuclei." Thesis, 2016. https://hdl.handle.net/2144/19197.

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The examination and processing of sexual assault evidence within the forensic science community has presented many challenges. Sexual assault evidence is often submitted as a heterogeneous mixture that requires separation of cell types for further analysis. The utilization of differential extractions provides a separation technique based on structural differences between epithelial cells (E-cells) and spermatozoa. Differential extraction does not separate cell types completely, as there may be carry over in both fractions. A protocol using several proteases was designed to separate cell type
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Book chapters on the topic "Sperm DNA Extraction"

1

Dash, Hirak Ranjan, Pankaj Shrivastava, and Surajit Das. "Differential Extraction of Sperm and Epithelial Cell DNA." In Springer Protocols Handbooks. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0274-4_10.

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2

Griffin, Jeanine. "Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-038-0_32.

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