Academic literature on the topic 'Spermatocytes'

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Journal articles on the topic "Spermatocytes"

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Brower, Jeffrey V., Chae Ho Lim, Marda Jorgensen, S. Paul Oh, and Naohiro Terada. "Adenine nucleotide translocase 4 deficiency leads to early meiotic arrest of murine male germ cells." REPRODUCTION 138, no. 3 (2009): 463–70. http://dx.doi.org/10.1530/rep-09-0201.

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Male fertility relies on the highly specialized process of spermatogenesis to continually renew the supply of spermatozoa necessary for reproduction. Central to this unique process is meiosis that is responsible for the production of haploid spermatozoa as well as for generating genetic diversity. During meiosis I, there is a dramatic increase in the number of mitochondria present within the developing spermatocytes, suggesting an increased necessity for ATP production and utilization. Essential for the utilization of ATP is the translocation of ADP and ATP across the inner mitochondrial membr
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Barchi, Marco, Shantha Mahadevaiah, Monica Di Giacomo, et al. "Surveillance of Different Recombination Defects in Mouse Spermatocytes Yields Distinct Responses despite Elimination at an Identical Developmental Stage." Molecular and Cellular Biology 25, no. 16 (2005): 7203–15. http://dx.doi.org/10.1128/mcb.25.16.7203-7215.2005.

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ABSTRACT Fundamentally different recombination defects cause apoptosis of mouse spermatocytes at the same stage in development, stage IV of the seminiferous epithelium cycle, equivalent to mid-pachynema in normal males. To understand the cellular response(s) that triggers apoptosis, we examined markers of spermatocyte development in mice with different recombination defects. In Spo11 − / − mutants, which lack the double-strand breaks (DSBs) that initiate recombination, spermatocytes express markers of early to mid-pachynema, forming chromatin domains that contain sex body-associated proteins b
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Gerard, N., A. Corlu, B. Kneip, et al. "Liver-regulating protein (LRP) is a plasma membrane protein involved in cell contact-mediated regulation of Sertoli cell function by primary spermatocytes." Journal of Cell Science 108, no. 3 (1995): 917–25. http://dx.doi.org/10.1242/jcs.108.3.917.

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We have identified a liver-regulating protein involved in cell contact-mediated regulation of Sertoli cell function by primary spermatocytes in rat testis. Liver-regulating protein was studied using monoclonal antibody L8 prepared from rat primitive biliary epithelial cells. This molecule was located in vivo at the interface of Sertoli cells and spermatocytes, and expressed in a stage-dependent manner (expression peaked on leptotene-zygotene spermatocytes). In vitro, the liver-regulating protein was found on Sertoli cell, spermatocyte and early spermatid membranes. Immunoaffinity procedures re
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Enders, G. C., and C. F. Millette. "Pachytene spermatocyte and round spermatid binding to Sertoli cells in vitro." Journal of Cell Science 90, no. 1 (1988): 105–14. http://dx.doi.org/10.1242/jcs.90.1.105.

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Spermatogenic cells differentiate in vivo while in continuous contact with the Sertoli cell. During differentiation, the spermatogenic cells and Sertoli cells form a number of morphologically distinct stage-specific adhesions. We describe an in vitro assay system for studying the adhesion of spermatogenic cells to Sertoli cell monolayers. Mixed populations of spermatogenic cells or enriched fractions of pachytene spermatocytes and round spermatids were labelled with the vital dye, fluorescein diacetate, prior to their addition to Sertoli cell monolayers so that the adhesion of viable spermatog
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Rodriguez, Valeria, Gabriela Diaz de Barboza, Ruben Ponce, Valeria Merico, Silvia Garagna, and Nori Tolosa de Talamoni. "Spermatocyte apoptosis, which involves both intrinsic and extrinsic pathways, explains the sterility of Graomys griseoflavus × Graomys centralis male hybrids." Reproduction, Fertility and Development 22, no. 2 (2010): 478. http://dx.doi.org/10.1071/rd09106.

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Spermatogenic impairment and the apoptotic pathways involved in establishing sterility of male hybrids obtained from crossing Graomys griseoflavus females with Graomys centralis males were studied. Testes from G. centralis, G. griseoflavus and hybrids were compared at different ages. Terminal transferase-mediated dUTP nick-end labelling assay (TUNEL), Fas, Bax and cytochrome c labelling were used for apoptosis evaluation, and calbindin D28k staining as an anti-apoptotic molecule. In 1-month-old animals, spermatocytes were positive for all apoptotic markers, but moderate TUNEL (+) spermatocyte
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Ferraro-Gideon, Jessica, Rozhan Sheykhani, Qingyuan Zhu, Michelle L. Duquette, Michael W. Berns, and Arthur Forer. "Measurements of forces produced by the mitotic spindle using optical tweezers." Molecular Biology of the Cell 24, no. 9 (2013): 1375–86. http://dx.doi.org/10.1091/mbc.e12-12-0901.

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We used a trapping laser to stop chromosome movements in Mesostoma and crane-fly spermatocytes and inward movements of spindle poles after laser cuts across Potorous tridactylus (rat kangaroo) kidney (PtK2) cell half-spindles. Mesostoma spermatocyte kinetochores execute oscillatory movements to and away from the spindle pole for 1–2 h, so we could trap kinetochores multiple times in the same spermatocyte. The trap was focused to a single point using a 63× oil immersion objective. Trap powers of 15–23 mW caused kinetochore oscillations to stop or decrease. Kinetochore oscillations resumed when
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Lee, K. P., and L. A. Kinney. "The infrastructure and Reversibility of Testicular Atrophy Induced by Ethylene Glycol Monomethyl Ether (EGME) in the Rat." Toxicologic Pathology 17, no. 4_part_2 (1989): 759–73. http://dx.doi.org/10.1177/0192623389017004204.

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Inhalation exposure to 300 ppm ethylene glycol monomethyl ether (EGME) for 3 days produced degenerative changes in spermatocytes of pachytene and meiotic division at spermatogenic stage XIV in rats. However, a wide range of germ cell types including spermatogonia was affected and the stage-specific damage was not discernible after 2 weeks exposure to 300 ppm EGME. The stage-specific damage was related to exposure concentration-time course. In early stages, degenerating spermatocytes showed nuclear chromatin clumping around synaptonemal complexes, cytoplasmic vesiculation with electron-dense ma
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Ogura, Atsuo, Teruhiko Wakayama, Osamu Suzuki, Tae-Young Shin, Junichiro Matsuda, and Yoshiro Kobayashi. "Chromosomes of mouse primary spermatocytes undergo meiotic divisions after incorporation into homologous immature oocytes." Zygote 5, no. 2 (1997): 177–82. http://dx.doi.org/10.1017/s096719940000383x.

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SummaryThe primary spermatocytes used were male germ cells at prophase I. The present study was undertaken to see whether bivalent chromosomes of mouse primary spermatocytes can undergo meiotic divisions within maturing oocytes and participate in subsequent embryonic development. Primary spermatocytes (pachytene to diplotene) freshly collected from the testes of mature males were electrofused with immature oocytes shortly before or after germinal vesicle breakdown. After culture in MEM-α medium for 15 h, most (> 90%) of the oocytes containing spermatocyte chromosomes underwent maturation an
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Guha, T., A. Q. Siddiqui, and P. F. Prentis. "Ultrastructure of primary spermatocyte in fish (Tilapia: Oreochromis niloticus): The synaptonemal complex." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 288–89. http://dx.doi.org/10.1017/s0424820100143067.

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The Primary Spermatocytes represent a stage in spermatogenesis when the first meiotic cell division occurs. They are derived from Spermatogonium or Stem cell through mitotic division. At the zygotene phase of meiotic prophase the Synaptonemal complex appears in these cells in the space between the paired homologous chromosomes. Spermatogenesis and sperm structure in fish have been studied at the electron microscope level in a few species? However, no work has yet been reported on ultrastructure of tilapia, O. niloticus, spermatozoa and spermatogenetic process. In this short communication we ar
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Li, Miao, Jiahuan Zheng, Gaopeng Li, et al. "The male germline-specific protein MAPS is indispensable for pachynema progression and fertility." Proceedings of the National Academy of Sciences 118, no. 8 (2021): e2025421118. http://dx.doi.org/10.1073/pnas.2025421118.

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Meiosis is a specialized cell division that creates haploid germ cells from diploid progenitors. Through differential RNA expression analyses, we previously identified a number of mouse genes that were dramatically elevated in spermatocytes, relative to their very low expression in spermatogonia and somatic organs. Here, we investigated in detail 1700102P08Rik, one of these genes, and independently conclude that it encodes a male germline-specific protein, in agreement with a recent report. We demonstrated that it is essential for pachynema progression in spermatocytes and named it male pachyn
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Dissertations / Theses on the topic "Spermatocytes"

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Redhouse, Juliet Lauren. "The nuclear organisation of Drosophila melanogaster primary spermatocytes." Thesis, University of Cambridge, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611384.

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Crichton, James Hugh. "Dissecting the meiotic defects of Tex19.1-/- mouse spermatocytes." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/21042.

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The maintenance of genomic stability through suppression of retrotransposon activity is vital for the avoidance of potentially mutagenic genomic disruption caused by retrotransposition. Germline development is a particularly important phase for retrotransposon silencing as retrotransposition events here have the potential for transmission to the entire embryo, threatening the health of offspring. A collection of germline genome defence genes are required for the suppression of retrotransposons in the developing germline of male mice (e.g. Tex19.1, Dazl, Mili, Miwi2, Gasz, Mov10l1, Mael, Dnmt3l
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Messina, Kari Lynn. "SPE-7, a Novel Regulator of MSP Assembly in C elegans Spermatocytes." W&M ScholarWorks, 2012. https://scholarworks.wm.edu/etd/1539626928.

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Lassen, Paula Graziela. "Morfometria e desenvolvimento das gônadas de tilápias (Oreochromis niloticus) suplementadas com sal mineral composto por cobre, manganês e zinco." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/143757.

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O objetivo deste trabalho foi o de avaliar o efeito da suplementação da dieta de Tilápias (Oreochromis niloticus) utilizando sal comercial composto dos microminerais cobre, manganês e zinco, sobre a histologia e o desenvolvimento das gônadas dos machos. Foram utilizados 1200 machos masculinizados de tilápia, com média de peso de 120g, suplementadas com níveis crescentes (0,00; 0,35; 0,70; 1,05; 1,40 e 1,75 mg/kg) de um produto comercial composto por Cu 13,40 g/kg; Mn 26,70 g/kg e Zn 66,70 g/kg. O experimento foi conduzido em um sistema de recirculação de água, composto por 24 tanques divididos
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Perrin, Jeanne. "La méïose spermatocytaire : anomalies méiotiques chez les hommes infertiles et évaluation d'un modèle d'étude in vitro." Aix-Marseille 2, 2008. http://www.theses.fr/2008AIX20661.

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L’analyse cytogénétique des chromosomes méiotiques révèle chez les Hommes infertiles des anomalies de ségrégation chromosomique, corrélées à la sévérité de l’altération de la spermatogenèse. Notre travail évalue le taux d’asynapsis dans les spermatocytes au stade pachytène de la méiose, chez des patients présentant une infertilité d’origine obstructive (O) ou non obstructive (NO) et chez un patient porteur d’une microdélétion AZFb du chromosome Y. 41 patients et 13 témoins sont étudiés : au total, 2931 noyaux pachytène sont analysés (coloration au Giemsa, immunofluorescence). Le taux moyen de
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Rousseaux, Sophie. "Ségrégations méiotiques des chromosomes remaniés dans les translocations humaines : analyse par hybridation in situ en fluorescence sur les noyaux spermatiques interphasiques." Université Joseph Fourier (Grenoble), 1995. http://www.theses.fr/1995GRE10065.

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Une translocation équilibrée résulte d'un échange de fragments entre deux chromosomes non homologues, sans perte ni gain de matériel génétique. La ségrégation méiotique des chromosomes de sujets porteurs de translocations est analysée par hybridation in situ en fluorescence (FISH) multicouleur sur noyaux spermatiques interphasiques. Elle a d'abord été étudiée dans 27 000 spermatozoïdes provenant de deux frères porteurs d'une translocation réciproque t(6 ;11) (q14 ;p14). Trois sondes centromériques, spécifiques des chromosomes 6, 11 et 1 ont été hybridées simultanément afin de détecter tous les
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SAIAS, MAGNAN JACQUELINE. "Reconstruction tridimensionnelle de noyaux de spermatocytes 1 au stade zygotene chez un sujet infertile porteur d'une translocation reciproque t(13;14)." Aix-Marseille 2, 1993. http://www.theses.fr/1993AIX20902.

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Guichaoua, Marie-Roberte. "L'infertilité masculine d'origine chromosomique : ses mécanismes : apport de l'étude du stade pachytène dans les spermatocytes I en prophase de Méiose." Aix-Marseille 2, 1990. http://www.theses.fr/1990AIX21904.

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Winters, Tristan. "The role of STAG3 in mammalian meiosis." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-233399.

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The cohesin complex is essential for mitosis and meiosis. The specific meiotic roles of individual cohesin proteins are incompletely understood. We report in vivo functions of the only meiosis-specific STAG component of cohesin, STAG3. Newly generated STAG3-deficient mice of both sexes are sterile with meiotic arrest. In these mice, meiotic chromosome architecture is severely disrupted as no bona fide axial elements (AE) form and homologous chromosomes do not synapse. Axial element protein SYCP3 forms dot-like structures, many partially overlapping with centromeres. Asynapsis marker HORMAD1 is
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Foster, Naomi. "The involvement of ARF6 in rapid membrane recycling during Drosophila spermatocyte cytokinesis." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1171479096001-41311.

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Book chapters on the topic "Spermatocytes"

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Guraya, Sardul S. "Spermatocytes." In Biology of Spermatogenesis and Spermatozoa in Mammals. Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71638-6_4.

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Callan, Harold Garnet. "Spermatocytes with Lampbrush Chromosomes." In Molecular Biology, Biochemistry and Biophysics. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-82792-1_7.

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Ko, Evelyn, and Renée H. Martin. "Immunofluorescence Analysis of Human Spermatocytes." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-103-5_23.

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Oliver-Bonet, Maria. "FISH on Sperms, Spermatocytes and Oocytes." In Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-70581-9_16.

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Oliver-Bonet, Maria. "FISH on Sperm, Spermatocytes and Oocytes." In Springer Protocols Handbooks. Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-52959-1_23.

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Yun, Yan, Masaru Ito, Sumit Sandhu, and Neil Hunter. "Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes." In Homologous Recombination. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0644-5_19.

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Lin, Ting-Yi, M. Jodeane Pringle, and Margaret T. Fuller. "Regulation of Meiosis and Spermatid Differentiation in Drosophila Primary Spermatocytes." In The Testis. Springer New York, 2000. http://dx.doi.org/10.1007/978-1-4612-2106-7_11.

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Thomas, Sharon E., and Bruce D. McKee. "Analysis of Chromosome Dynamics and Chromosomal Proteins in Drosophila Spermatocytes." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-103-5_13.

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Savoian, Matthew S. "Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6340-9_15.

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La Salle, Sophie, Fengyun Sun, and Mary Ann Handel. "Isolation and Short-Term Culture of Mouse Spermatocytes for Analysis of Meiosis." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-103-5_17.

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Conference papers on the topic "Spermatocytes"

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Atsayeva, Maret M., Zura I. Bisultanova, Petimat M. Dzhambetova, Nadezhda Yu Oyun, and Oksana L. Kolomiets. "The Study of the Transgeneration Genotoxic Effect of Drugs Based on the Analysis of Synaptonemal Complexes in Mouse Spermatocytes." In Proceedings of the International Symposium “Engineering and Earth Sciences: Applied and Fundamental Research” (ISEES 2018). Atlantis Press, 2018. http://dx.doi.org/10.2991/isees-18.2018.27.

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