Academic literature on the topic 'Spermatozoo'

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Journal articles on the topic "Spermatozoo"

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Paoli, Donatella, Tania Carlini, Fabiana Faja, Monica Muratori, and Elisabetta Baldi. "Metodi di valutazione del danno al DNA dello spermatozoo." L'Endocrinologo 19, no. 4 (August 2018): 179–84. http://dx.doi.org/10.1007/s40619-018-00465-1.

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Zhao, Bin, and Bing Liu. "Ultrastructure of the spermatid and spermatozoon of Macracanthorhynchus hirudinaceus." Journal of Helminthology 66, no. 4 (December 1992): 267–72. http://dx.doi.org/10.1017/s0022149x0001470x.

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ABSTRACTThe ultrastructure of the spermatid and spermatozoon of Macracanthorhynchus hirudinaceus (Archiacanthocephala) was studied by means of transmission electron microscopy. The flagellum and nucleus in the spermatid gradually expanded simultaneously. The karyoplasm of the spermatid transformed into dense inclusions and a multibarrel structure, which were also found in the spermatozoan body. The multibarrel structure was located close to the flagellum and consisted of many irregular microtubes. The flagellum of the developing spermatozoon was observed in a concavity of the spermatid nucleus. The microtubule arrangement of the flagellum was ″9+2″. No mitochondria or acrosome were observed in spermatozoa.
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Valdivia, M., T. Sillerico, A. De Ioannes, and C. Barros. "Proteolytic activity of rabbit perivitelline spermatozoa." Zygote 7, no. 2 (May 1999): 143–49. http://dx.doi.org/10.1017/s0967199499000507.

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Acrosin, an acrosomal serine protease, has been associated with binding of spermatozoa and their penetration through the zona pellucida. This study was aimed at determining whether the remaining proacrosin/acrosin system on rabbit perivitelline spermatozoa still has proteolytic activity and whether this activity is involved in further penetration of unfertilised rabbit eggs. Eight hundred and sixty-five rabbit perivitelline spermatozoa were evaluated by the gelatin-substrate film technique for the detection of acrosin on individual spermatozoan. Fifteen per cent of the studied spermatozoa showed small digestion halos on the gelatin film. The proteolytic activity of rabbit perivitelline spermatozoa was inhibited in the presence of 1 mg/ml of soybean trypsin inhibitor (SBTI) or with 20 μg/ml of a mixture of the monoclonal anti-proacrosin/acrosin antibody. In vitro fertilisation occurred in 21.8% of rabbit oocytes co-incubated with perivitelline spermatozoa and was completely inhibited when oocytes were incubated with 600 μg/ml of a mixture of three anti-acrosin monoclonal antibodies (ACRO-A8C10, ACRO-C2B10 and ACRO-C5F10). Inseminations in the presence of anti-cholera monoclonal antibody (irrelevant to spermatozoa) resulted in 17.6% fertilisation. These results support the idea that the residual proacrosin/acrosin system in perivitelline spermatozoa might be involved in spermatozoal binding and/or second penetration through the zona pellucida.
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Tosit, Elisabetta. "Sperm activation in species with external fertilisation." Zygote 2, no. 4 (November 1994): 359–61. http://dx.doi.org/10.1017/s0967199400002215.

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The spermatoozoon is an excitable cell that responds to specific effectors by rapidly changing its behaviour. In species with external fertilisation, spermatozoa are stored in a quiescent state in the testis, but within seconds after spawning, dilution into water triggers several activation events such as increases in motility and respiration. In some species, these are followed by the acrosome reaction, an exocytotic process that allows the spermatozoon to penetrate the egg investments and activate the egg (Dale, 1983). The majority of information on sperm activation has come from the sea urchin; secondarily teleosts and starfish have proved to be useful models.
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Ishijima, S., M. S. Hamaguchi, M. Naruse, S. A. Ishijima, and Y. Hamaguchi. "Rotational movement of a spermatozoon around its long axis." Journal of Experimental Biology 163, no. 1 (February 1, 1992): 15–31. http://dx.doi.org/10.1242/jeb.163.1.15.

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The rotational movement of a spermatozoon around its longitudinal axis was investigated by two methods: by observing a spermatozoon attached vertically to a coverslip by the tip of its head, and by observing a spermatozoon freely swimming in a medium by means of ‘double-focal microscopy’, which yielded simultaneous images at two different focal planes. Similar results were obtained by these two methods. Sea urchin, starfish, medaka, human, golden hamster and bull spermatozoa rolled in both clockwise and counterclockwise directions, although there was a large difference in the proportion of spermatozoa rolling in each direction in the different species. The majority of sea urchin and starfish spermatozoa rolled in a clockwise direction when an observer viewed the cell from its anterior end, whereas the majority of medaka, golden hamster, human and bull spermatozoa rolled in a counterclockwise direction relative to the same observer. Moreover, some spermatozoa occasionally changed their rotational direction. These results suggest that the mechanism regulating the direction of rotation of the spermatozoa is lax. As rotational movement of a spermatozoon around its longitudinal axis is due to the three-dimensional component of the beat of the flagellum, the direction of the three-dimensional movement presumably changes as the spermatozoa swim.
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Hayashi, Masayuki, Naoto Yonezawa, Toshiyuki Katsumata, Keiichi Ikeda, Fabiana Lica Imai, Kazuhiro Kikuchi, Seizo Hamano, and Minoru Nakano. "Activity of exoglycosidases in ejaculated spermatozoa of boar and bull." Zygote 12, no. 2 (May 2004): 105–9. http://dx.doi.org/10.1017/s0967199404002692.

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The activity of exoglycosidases in extracts from freshly ejaculated boar and bull spermatozoa with 0.2% Brij-35/2% acetic acid was measured. The results show that β-N-acetylhexosaminidase, β-galactosidase and α-mannosidase are the major glycosidases; much higher levels of activity were found in boar spermatozoa than in bull spermatozoa. When compared on a per spermatozoon basis, the ratios of the activities of β-N-acetylhexosaminidase, β-galactosidase and α-mannosidase in boar spermatozoon relative to those in bull spermatozoon were approximately 13 000:1, 1700:1 and 400:1, respectively. Liberation of these glycosidases from bull spermatozoa by treatment with phosphatidylinositol-specific phospholipase C (PI-PLC) was low, in contrast to liberation of α-mannosidase from boar spermatozoa previously found by the same means. The possibility that the exoglycosidases present in large amounts in boar spermatozoa play a role in the process of binding to the zona pellucida glycoprotein of the egg is discussed.
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Andraszek, Katarzyna, and Elżbieta Smalec. "The use of silver nitrate for the identification of spermatozoon structure in selected mammals." Canadian Journal of Animal Science 91, no. 2 (June 2011): 239–46. http://dx.doi.org/10.4141/cjas10052.

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Andraszek, K. and Smalec, E. 2011. The use of silver nitrate for the identification of spermatozoon structure in selected mammals. Can. J. Anim. Sci. 91: 239–246. The spermatozoon is one of the most diversified cell types, and the chromatin of the haploid spermatozoon genome is essentially different from that of the somatic cell as regards its chemical composition, structure and function. Although the structure of spermatozoon chromatin has crucial importance for fertilization and embryo development, standard staining techniques are still predominantly used for identifying semen quality and the assessment of spermatozoa is most often limited to detecting irregularities in their morphological structure. The aim of the present research was to evaluate the usefulness of silver nitrate staining for assessing spermatozoon morphology and identifying spermatozoon structure. Spermatozoa isolated from testes and semen were examined. Silver nitrate staining made it possible to identify many significant details of the morphological structure of the spermatozoon and could be successfully employed in sperm morphology assessments.
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Kondracki, S., A. Wysokińska, M. Iwanina, D. Banaszewska, and D. Sitarz. "Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars." Polish Journal of Veterinary Sciences 14, no. 1 (December 1, 2011): 35–40. http://dx.doi.org/10.2478/v10181-011-0005-z.

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Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars The experimental material consisted of 75 ejaculates collected form 8 Duroc boars. The ejaculates were divided into three groups according to sperm concentration in an ejaculate. An ejaculate was obtained from each boar monthly and it was used to make microscopic preparations to examine spermatozoa morphology. In each preparation morphometric measurements were taken of fifteen randomly selected spermatozoa characterized by normal morphology. The following measurements of spermatozoa were taken: length and width of the spermatozoa head, head area, length of the flagellum, perimeter of the spermatozoon head and total spermatozoon length. The results were used to calculate indicators of spermatozoa morphology. Moreover, assessments were made of frequency of morphological defects to isolate spermatozoa with primary and secondary abnormalities following the Blom classification system. It was found that the concentration of spermatozoa in the ejaculate influenced the morphometric characteristics of spermatozoa. Ejaculates with low sperm concentrations are characterized by larger spermatozoa as compared to ejaculates with high sperm concentrations. However, sperm concentration in the ejaculate does not much influence the shape of spermatozoa.
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Lacham-Kaplan, O., and AO Trounson. "Embryo development capacity of oocytes fertilized by immature sperm and sperm treated with motility stimulants." Reproduction, Fertility and Development 6, no. 1 (1994): 113. http://dx.doi.org/10.1071/rd9940113.

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The fertilizing ability of mouse spermatozoa develops during maturation and coincides with the acquisition of motility. The lack of progressive motility of spermatozoa from the testis and precaudal segments of the epididymis interferes with their ability to fertilize oocytes after insemination in vitro. The removal of cumulus cells for insemination in vitro and the use of subzonal injection of a single spermatozoon resulted in a higher number of oocytes fertilized by immature caput and corpus spermatozoa. High rates of embryonic arrest and retarded development were observed in oocytes fertilized by caput and corpus spermatozoa when compared with oocytes fertilized by cauda spermatozoa. However, when the oocytes were enclosed in their cumulus cells or microinjected with a single spermatozoon, these effects were reduced. A block in embryonic development was also observed after human and mouse oocytes were exposed to the sperm motility stimulants pentoxifylline (PTF) and 2-deoxyadenosine (DOA). These observations suggest that exposure of oocytes to PTF and DOA should be avoided.
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Miller, David, Martin Brinkworth, and David Iles. "Paternal DNA packaging in spermatozoa: more than the sum of its parts? DNA, histones, protamines and epigenetics." REPRODUCTION 139, no. 2 (February 2010): 287–301. http://dx.doi.org/10.1530/rep-09-0281.

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Haploid male germ cells package their DNA into a volume that is typically 10% or less that of a somatic cell nucleus. To achieve this remarkable level of compaction, spermatozoa replace most of their histones with smaller, highly basic arginine and (in eutherians) cysteine rich protamines. One reason for such a high level of compaction is that it may help optimise nuclear shape and hence support the gametes' swimming ability for the long journey across the female reproductive tract to the oocyte. Super-compaction of the genome may confer additional protection from the effects of genotoxic factors. However, many species including the human retain a fraction of their chromatin in the more relaxed nucleosomal configuration that appears to run counter to the ergonomic, toroidal and repackaging of sperm DNA. Recent research suggests that the composition of this ‘residual’ nucleosomal compartment, a generally overlooked feature of the male gamete, is far more significant and important than previously thought. In this respect, the transport and incorporation of modified paternal histones by the spermatozoon to the zygote has been demonstrated and indicates another potential paternal effect in the epigenetic reprogramming of the zygote following fertilisation that is independent of imprinting status. In this review, the most recent research into mammalian spermatozoal chromatin composition is discussed alongside evidence for conserved, non-randomly located nucleosomal domains in spermatozoal nuclei, all supporting the hypothesis that the spermatozoon delivers a novel epigenetic signature to the egg that may be crucial for normal development. We also provide some thoughts on why this signature may be required in early embryogenesis.
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Dissertations / Theses on the topic "Spermatozoo"

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Cova, Riccardo. "Analisi di dati citofluorimetrici con tecniche di Data Mining." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amslaurea.unibo.it/4774/.

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Il citofluorimetro è uno strumento impiegato in biologia genetica per analizzare dei campioni cellulari: esso, analizza individualmente le cellule contenute in un campione ed estrae, per ciascuna cellula, una serie di proprietà fisiche, feature, che la descrivono. L’obiettivo di questo lavoro è mettere a punto una metodologia integrata che utilizzi tali informazioni modellando, automatizzando ed estendendo alcune procedure che vengono eseguite oggi manualmente dagli esperti del dominio nell’analisi di alcuni parametri dell’eiaculato. Questo richiede lo sviluppo di tecniche biochimiche per la marcatura delle cellule e tecniche informatiche per analizzare il dato. Il primo passo prevede la realizzazione di un classificatore che, sulla base delle feature delle cellule, classifichi e quindi consenta di isolare le cellule di interesse per un particolare esame. Il secondo prevede l'analisi delle cellule di interesse, estraendo delle feature aggregate che possono essere indicatrici di certe patologie. Il requisito è la generazione di un report esplicativo che illustri, nella maniera più opportuna, le conclusioni raggiunte e che possa fungere da sistema di supporto alle decisioni del medico/biologo.
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Godo, Pla Anna. "Anàlisi del contingut cromosòmic en espermatozoides d’individus portadors de translocacions: relació entre efecte intercromosòmic i segregació." Doctoral thesis, Universitat Autònoma de Barcelona, 2015. http://hdl.handle.net/10803/318798.

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Els individus portadors de translocacions cromosòmiques equilibrades presenten un risc incrementat de produir gàmetes amb anomalies cromosòmiques, ja sigui per una segregació desequilibrada dels cromosomes reorganitzats o per la formació d’anomalies numèriques derivades de l’efecte intercromosòmic. Es desconeix si existeix una relació entre les anomalies derivades d’ambdós esdeveniments, que podria residir en l’aparició de fenòmens d’heterosinapsi entre els multivalents i altres cromosomes durant la profase I. D’altra banda, l’heterosinapsi s’ha relacionat amb canvis en l’arquitectura nuclear dels cromosomes en espermatozoides, que en última instància també podrien afectar la fertilitat dels individus portadors. Els objectius d’aquesta tesi han estat: i) Desenvolupar un mètode d’anàlisi seqüencial basat en tècniques d’hibridació in situ fluorescent (FISH) que permeti identificar anomalies cromosòmiques numèriques i determinar el mode de segregació dels cromosomes reorganitzats sobre els mateixos espermatozoides; ii) Caracteritzar els patrons de segregació de diferents reorganitzacions cromosòmiques en espermatozoides no seleccionats i en espermatozoides portadors d’anomalies numèriques; iii) Determinar si existeix una relació entre els diferents modes de segregació dels cromosomes reorganitzats i la producció d’espermatozoides amb anomalies numèriques; iv) Desenvolupar una tècnica d’anàlisi que permeti valorar la disposició tridimensional dels territoris cromosòmics en nuclis d’espermatozoides. S’han recollit mostres de semen de vuit individus portadors de translocacions recíproques, onze portadors de translocacions Robertsonianes i un portador d’una triple translocació. En la primera ronda de FISH seqüencial s’han detectat espermatozoides amb anomalies numèriques per cinc cromosomes no relacionats amb la reorganització. En la segona ronda s’han analitzat els productes de segregació dels cromosomes reorganitzats en els espermatozoides amb anomalies numèriques detectats prèviament, així com en espermatozoides no seleccionats. L’optimització del sistema d’anàlisi de la disposició dels territoris cromosòmics en el nucli espermàtic ha inclòs la selecció de nuclis segons el seu genotip, la captura d’imatges tridimensionals, la relocalització dels nuclis d’interès, el tractament digital de les imatges, la normalització de les dades i l’obtenció dels valors de posicionament dels centròmers en l’eix longitudinal i radial de l’espermatozoide. Els resultats de l’anàlisi de segregació en espermatozoides no seleccionats mostren una elevada homogeneïtat dels patrons de segregació entre portadors del mateix tipus de translocació, caracteritzats per una elevada freqüència dels modes de segregacions que impliquen la disjunció a pols cel·lulars oposats dels cromosomes amb centròmers homòlegs. Els resultats de segregació en espermatozoides aneuploides i diploides/múltiples disòmics mostren un patró de segregació alterat respecte els espermatozoides no seleccionats, on s’afavoreixen els modes de segregació desequilibrats, en detriment dels productes de segregació equilibrats. Els espermatozoides amb diferents tipus d’aneuploïdies i amb diferents cromosomes implicats, influeixen per igual al patró de segregació alterat. Els resultats sobre el posicionament tridimensional dels cromosomes han permès validar la metodologia desenvolupada, ja que s’ha obtingut informació sobre la localització preferent dels cromosomes estudiats en nuclis amb diferents genotips. Els resultats obtinguts demostren una associació entre la presència d’anomalies numèriques i un contingut desequilibrat dels cromosomes reorganitzats. L’acumulació d’anomalies cromosòmiques en els mateixos gàmetes pot ser atribuïble a un efecte bidireccional de l’heterosinapsi, que comportaria un canvi en la localització nuclear dels cromosomes afectats i una alteració en la formació dels quiasmes. Els problemes d’orientació dels cromosomes a la placa metafàsica poden provocar una aturada meiòtica prolongada, que en cas de no corregir-se, es podria resoldre a través de l’evasió del punt de control. Aquest fet afavoriria la producció de gàmetes que podrien acumular anomalies procedents de segregacions desequilibrades, a més d’aneuploïdies d’altres cromosomes. La tècnica desenvolupada per estudiar l’arquitectura nuclear dels espermatozoides permetrà determinar si les anomalies cromosòmiques presents en els gàmetes dels individus portadors de reorganitzacions cromosòmiques afecten l’organització nuclear global dels cromosomes i condicionen la seva fertilitat.
Carriers of chromosomal translocations present a high risk of producing chromosomally abnormal gametes, as a consequence of an unbalanced segregation of the rearranged chromosomes, or the presence of numerical chromosomal anomalies derived from an interchromosomal effect. It is not known whether there exists a relationship between anomalies produced by these two events, but it might be based on the occurrence of heterosynapsis at the meiotic prophase I between multivalents and other chromosomes. Moreover, heterosynapsis has been associated related to changes in the nuclear chromosome architecture in sperm, which may also affect the fertility of reorganization carriers. The objectives of this thesis have been: i) To develop a sequential fluorescence in situ hybridization (FISH) protocol in order to detect numerical chromosomal abnormalities and to establish the segregation mode of rearranged chromosomes in the same spermatozoa; ii) To establish the segregation pattern of different chromosomal rearrangements in random sperm and in sperm with numerical abnormalities; iii) To determine whether there exists a relationship between certain segregation modes and the occurrence of numerical chromosome abnormalities; iv) To develop a methodology to evaluate the tridimensional distribution of chromosome territories in sperm nuclei. Semen samples of eight carriers of reciprocal translocations, eleven carriers of Robertsonian translocations and one carrier of a three-way translocation have been included in the study. In the first sequential FISH round, numerical anomalies for five chromosomes unrelated to the rearrangements have been analysed. In the second round, a segregation analysis has been performed both in the numerically abnormal sperm detected in the first round as well as in randomly assessed sperm. The optimization of the analysis of chromosome territories in sperm nuclei has included: nuclei classification according to their genotype, 3D image recording, relocalization of selected nuclei, digital images editing, data normalization, and prediction of the preferred position of each hybridization signal along the longitudinal and radial axis of spermatozoa. The segregation patterns obtained in randomly assessed sperm show high homogeneity among carriers of the same translocation. These patterns involve high frequencies of segregation modes that entail disjunction to opposite cellular poles of chromosomes with homologous centromeres. Data obtained from segregation analysis in aneuploid and diploid/multiple disomic sperm show altered segregation patterns when compared to randomly assessed sperm, in which unbalanced segregation modes are favoured while balanced segregation products decrease. Aneuploid sperm with different types of chromosomal abnormalities or different chromosomes involved in the aneuploidy have the same effect over the altered segregation pattern. The results obtained in the analysis of chromosome territories allow for the validation of the developed methodology. It has allowed the prediction of the preferred positioning of analysed chromosomes in sperm nuclei with different genotypes. In conclusion, data obtained point out that indeed there exists a relationship between the presence of numerical chromosome abnormalities and an unbalanced segregation content in sperm. This accumulation of chromosome anomalies in the same gametes would be driven by a bidirectional effect of heterosynapsis, which could entail changes in the nuclear positioning of the affected chromosomes, as well as alterations in chiasmata formation. The chromosome misalignment at metaphase plate would cause a meiotic arrest. If unresolved, cells could eventually evade this checkpoint favouring the accumulation of both unbalanced segregation products and aneuploidies for other chromosomes in the same gametes. The developed methodology to study the sperm nuclear architecture can be potentially used in carriers of chromosomal rearrangements, in order to assess whether chromosomal abnormalities in sperm affect the global nuclear chromosome organization and disturb their fertility.
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Bradley, Leanne Teresa. "Cryopreservation of bovine spermatozoa." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0016/MQ47313.pdf.

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歐陽鎮怡 and Chun-yee Natalie Auyeung. "Fucosyltransferase-5 on human spermatozoa mediates attachment of spermatozoa to oviductal epithelial cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B40738620.

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Auyeung, Chun-yee Natalie. "Fucosyltransferase-5 on human spermatozoa mediates attachment of spermatozoa to oviductal epithelial cells." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B40738620.

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Williams, Andrew C. "Glucose metabolism in human spermatozoa." Thesis, University of Bristol, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302101.

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Pandolfi, Susan M. "Cryopreservation of microencapsulated bovine spermatozoa." Thesis, This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-11012008-063722/.

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Casey, Shannon Marie. "Effects of laundering on spermatozoa." Thesis, Boston University, 2012. https://hdl.handle.net/2144/12314.

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Thesis (M.S.)--Boston University PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
Collection of physical evidence as soon as possible after a sexual assault is important for obtaining the best evidence of the crime. Actions taken following a sexual assault, such as laundering clothing worn during the assault, may occur before evidence collection has begun, and can have an effect on the forensic laboratory analysis. The purpose of this study was to investigate the effects of laundering on spermatozoa. The effects of All®, Woolite® and bleach on sperm removal during washing, on sperm elution of previously laundered evidence samples, and on sperm transfer during washing were examined and compared. The possibility of sperm transfer between laundry loads was also investigated. Bleach was found to have a significant effect on sperm removal during simulated laundering. The use of detergents during washing did not prevent the subsequent elution of sperm with sodium dodecyl sulfate (SDS), and did not affect the amount of sperm transferred during washing. It was also found that sperm transfer between laundry loads can occur. These results should be considered when dealing with sexual assault evidence that has been laundered prior to forensic examination. Complications with DNA analysis on clothing may be possible due to the occurrence of sperm transfer during laundering either before or after a sexual assault occurs.
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Lewis, Beverley Anne. "Cell biology of rat spermatozoa." Thesis, University of Edinburgh, 2001. http://hdl.handle.net/1842/23087.

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The purpose of the research presented in this thesis was to investigate the cell biology of rat spermatozoa. An additional aim was to utilise the knowledge obtained to aid the development of in vitro functional tests for the assessment of rat sperm fertility and identify potential markers of normal epididymal maturation. As mammalian spermatozoa migrate through the epididymis, they acquire the potential for fertilisation, characterised by the acquisition of the ability to express co-ordinated movement and the competence to undergo capacitance. The mechanisms by which epididymal maturation confers upon mammalian spermatozoa the potential to capacitate is poorly understood. These studies investigated the impact of epididymal maturation on the signal transduction pathways regulating tyrosine phosphorylation using the laboratory rat as an animal model, since this signal transduction pathway is thought to be central to the attainment of a capacitated state and expression of hyperactivated motility, both of which are prerequisites for fertilisation. Western Blot and immunocytochemical analysis demonstrated that epididymal maturation is associated with a progressive loss in phosphotyrosine expression located to the acrosomal domain. These differences in phosphotyrosine expression between caput and caudal epididymal spermatozoa appeared to reflect the normal in vivo situation. In addition, epididymal maturation of rat spermatozoa is also associated with an acquired competence to respond to high levels of intracellular cAMP by phoshorylating tyrosine residues on the sperm tail. Epididymal maturation also led to unique differences in the generation of reactive oxygen species (ROS) by spermatozoa obtained from the caput and caudal regions of the epididymis. Spermatozoa from both regions of the epididymis spontaneously generated equal levels of O2 whereas only mature caudal spermatozoa generated significant levels of H2O2. In contrast, although both caput and caudal spermatozoa generated increased O2-. in response to NADPH, induced levels were significantly greater in the immature caput cells.
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Herold, Florian-Cecil. "Influence of equilibration time and freezing diluent on post-thaw motility and acrosomal integrity of epididymal sperm from the African buffalo (Syncerus caffer)." Pretoria : [s.n.], 2008. http://upetd.up.ac.za/thesis/available/etd-10032005-094824/.

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Books on the topic "Spermatozoo"

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S, Roldan Eduardo R., and Gomendio M, eds. Spermatology: Proceedings of the 10th International Symposium on Spermatology, held at El Escorial, Madrid, Spain, 17-22 September 2006. Nottingham, U.K: Nottingham University Press, 2007.

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Barth, A. D. Abnormal morphology of bovine spermatozoa. Ames: Iowa State University Press, 1989.

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Barth, A. D. Abnormal morphology of bovine spermatozoa. Ames: Iowa State University Press, 1989.

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Baldi, Elisabetta, and Monica Muratori, eds. Genetic Damage in Human Spermatozoa. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-7783-9.

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Baldi, Elisabetta, and Monica Muratori, eds. Genetic Damage in Human Spermatozoa. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21664-1.

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Pandya, Ila Jagannath. Human cervix, secretions and spermatozoa. Birmingham: University of Birmingham, 1987.

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Lejeune, Thomas. Human spermatozoa: Maturation, capacitation and abnormalities. New York: Nova Biomedical Books, 2010.

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Adeghe, Aimua Jude-Harris. Spermatozoal antibodies in male subfertility. Birmingham: University ofBirmingham, 1987.

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Jamieson, Barrie G. M. The ultrastructure and phylogeny of insect spermatozoa. Cambridge: Cambridge University Press, 1987.

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Guraya, Sardul S. Biology of spermatogenesis and spermatozoa in mammals. Berlin: Springer-Verlag, 1987.

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Book chapters on the topic "Spermatozoo"

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Srivastava, N., Megha Pande, S. Tyagi, and Omer Din. "Selection of Spermatozoa." In Protocols in Semen Biology (Comparing Assays), 7–17. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5200-2_2.

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Pacey, Allan, and Katrina Williams. "The Human Spermatozoa." In Clinical Reproductive Science, 65–73. Chichester, UK: John Wiley & Sons, Ltd, 2018. http://dx.doi.org/10.1002/9781118977231.ch5.

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Gandini, Loredana, Francesco Pallotti, Donatella Paoli, and Andrea Lenzi. "Cryopreservation of Spermatozoa." In Endocrinology, 1–16. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-29456-8_41-1.

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Salisbury, G. W., and J. R. Lodge. "Metabolism of Spermatozoa." In Advances in Enzymology - and Related Areas of Molecular Biology, 35–104. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470124888.ch2.

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Bien, Christian G., Christian E. Elger, Ali R. Afzal, Sirajedin Natah, Ritva Häyrinen-Immonen, Yrjö Konttinen, George S. Zubenko, et al. "Round-headed Spermatozoa." In Encyclopedia of Molecular Mechanisms of Disease, 1873. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-29676-8_8598.

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Heppner, John B., David B. Richman, Steven E. Naranjo, Dale Habeck, Christopher Asaro, Jean-Luc Boevé, Johann Baumgärtner, et al. "Spermatid (pl., spermatozoa)." In Encyclopedia of Entomology, 3484. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_4303.

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Neri, Queenie V., Jennifer Hu, Zev Rosenwaks, and Gianpiero D. Palermo. "Understanding the Spermatozoon." In Methods in Molecular Biology, 91–119. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0659-8_5.

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Gandini, Loredana, Francesco Pallotti, Donatella Paoli, and Andrea Lenzi. "Cryopreservation of Spermatozoa." In Endocrinology, 1235–50. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-44441-3_41.

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Briz, M., and A. Fàbrega. "The Boar Spermatozoon." In Boar Reproduction, 3–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-35049-8_1.

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Nixon, Brett, and R. John Aitken. "Proteomics of Human Spermatozoa." In Immune Infertility, 3–12. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-01379-9_1.

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Conference papers on the topic "Spermatozoo"

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Sitepu, Sukma Aditya, and Julia Marisa. "Pengaruh Penambahan Gentamisin dan Minyak Atsiri Jeruk Manis Terhadap Persentase Hidup Spermatozoa Pada Pengencer Semen Beku Sapi Simmental." In Kedaulatan Pangan Nasional Melalui Pengembangan Potensi Ternak Lokal di Era Kenormalan Baru. Animal Science : Polije Proceedings Series, 2020. http://dx.doi.org/10.25047/proc.anim.sci.2020.11.

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Penelitian ini bertujuan untuk mengetahui nilai persentase spermatozoa hidup pada semen beku sapi Simmental dengan menambahkan gentamisin dan minyak atsiri jeruk manis pada bahan pengencer tris kuning telur. Bahan yang digunakan dalam penelitian ini adalah semen segar sapi Simmental, pengencer tris kuning telur dan minyak atsiri jeruk manis. Pengencer tris kuning telur dengan menggunakan 3,32 g Tris (hidroksimetilaminometana); 1,86 g asam sitrat; 1,37 g fruktosa; 6 ml gliserol; 20 ml kuning telur; dan 100 ml aquades. Metode penelitian yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan. Perlakuan yang diberikan adalah penambahan minyak atsiri jeruk manis 0% (P0); 0,25% (P1); 0,5% (P2); 0,75% (P3) dan 1% (P4). Hasil penelitian menunjukkan bahwa semakin banyak penambahan minyak atsiri jeruk manis, persentase hidup spermatozoa pada semen beku Sapi Simmental akan terus meningkat. Hasil terbaik ditunjukkan pada penambahan 1% (P4) minyak atsiri jeruk manis dengan persentase hidup spermatozoa sebesar 88% (pra pembekuan) dan 75% (pasca pembekuan).
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Manago, S., G. Coppola, M. A. Ferrara, L. Sirleto, I. Rendina, R. Puglisi, D. Balduzzi, A. Galli, P. Ferraro, and A. C. De Luca. "Raman sex sorting of bovine spermatozoa." In 2014 Fotonica AEIT Italian Conference on Photonics Technologies (Fotonica AEIT). IEEE, 2014. http://dx.doi.org/10.1109/fotonica.2014.6843968.

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Halimah, Khori, Ari Yuniastuti, and Sri Rahayu. "Effect of Temperature on Spermatozoa Morphology." In Proceedings of the 5th International Seminar of Public Health and Education, ISPHE 2020, 22 July 2020, Universitas Negeri Semarang, Semarang, Indonesia. EAI, 2020. http://dx.doi.org/10.4108/eai.22-7-2020.2300287.

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Sørensen, Lauge, Jakob Østergaard, Peter Johansen, and Marleen de Bruijne. "Multi-object tracking of human spermatozoa." In Medical Imaging, edited by Joseph M. Reinhardt and Josien P. W. Pluim. SPIE, 2008. http://dx.doi.org/10.1117/12.771135.

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Okumus, Fatih, Adnan Fatih Kocamaz, and Mustafa Erkan Ozgur. "Detection and counting of Oncorhynchus Mykiss spermatozoa." In 2015 23th Signal Processing and Communications Applications Conference (SIU). IEEE, 2015. http://dx.doi.org/10.1109/siu.2015.7130205.

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Khachane, Monali Y., R. R. Manza, and R. J. Ramteke. "Fuzzy rule based classification of human spermatozoa." In 2015 International Conference on Electrical, Electronics, Signals, Communication and Optimization (EESCO). IEEE, 2015. http://dx.doi.org/10.1109/eesco.2015.7253656.

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AFIATI, FIFI. "Abnormalitas spermatozoa domba dengan frekuensi penampungan berbeda." In Seminar Nasional Masyarakat Biodiversitas Indonesia. Masyarakat Biodiversitas Indonesia, 2015. http://dx.doi.org/10.13057/psnmbi/m010449.

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Revollo Sarmiento, Natalia Verónica, Felix S. L. Thomsen, CLAUDIO DELRIEUX, and Rolando González-José. "Supervised learning for semantic segmentation of human spermatozoa." In 15th International Symposium on Medical Information Processing and Analysis, edited by Jorge Brieva, Eduardo Romero, and Natasha Lepore. SPIE, 2020. http://dx.doi.org/10.1117/12.2542464.

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Zhu, Anding, and Shunren Xia. "Segmentation methods of spermatoza microscopic image." In Second International Conference on Image and Graphics, edited by Wei Sui. SPIE, 2002. http://dx.doi.org/10.1117/12.477193.

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Alapati, Raghava, Michael Stout, Robert A. Godke, and Ram V. Devireddy. "Comparative Freezing Response of Ejaculated and Epididymal Bovine Spermatozoa." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192329.

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In the present study, we report the effects of cooling ejaculated and epididymal bovine sperm from the same animals with and without a cryoprotective agent, glycerol. Water transport data during freezing of ejaculated and epididymal bovine sperm cell suspensions were obtained at a cooling rate of 20 °C/min under two different conditions: i) in the absence of cryoprotective agents, CPAs; and ii) in the presence of 0.7 M glycerol. Using previously published values, the bovine sperm cell was modeled as a cylinder of length 39.8 μm and a radius of 0.4 μm with an osmotically inactive cell volume, Vb, of 0.61Vo, where Vo is the isotonic cell volume. The subzero water transport response is analyzed to determine the variables governing the rate of water loss during cooling of bovine spermatozoa, i.e. the membrane permeability parameters (reference membrane permeability, Lpg and activation energy, ELp). The predicted best-fit permeability parameters ranged from, Lpg = 0.021 to 0.038 μm/min-atm and ELp = 27.8 to 41.1 kcal/mol. The subzero water transport response and consequently the subzero water transport parameters are not significantly different between the ejaculated and epididymal bovine spermatozoa under corresponding cooling conditions. If this observation is found to be more generally valid for other mammalian species as well, then the sperm extracted from the testicles of an animal during post-mortem can also be optimally cryopreseved using procedures similar to those derived for ejaculated sperm.
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Reports on the topic "Spermatozoo"

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Andreeva, Madlena, Nikola Metodiev, Paulina Taushanova, and Rossen Stefanov. Influence of Cryopreservation on the Velocity Parameters of Spermatozoa from Breeds of Lacaune and Ile De France Sheep. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, December 2018. http://dx.doi.org/10.7546/crabs.2018.12.18.

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Dzhoglov, Spas, Doychin Boyadzhiev, and Evgeniya Neshova Ivanova. Association between Some Environment and Lifestyle Factors with Male Semen Quality Parameters: Semen Volume, Spermatozoa Concentra tion and Motility. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, January 2021. http://dx.doi.org/10.7546/crabs.2021.01.15.

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