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Journal articles on the topic 'Splinkerette'

Author: Grafiati

Published: 14 March 2023

Last updated: 31 July 2025

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1

Hengen, P. "Vectorette, splinkerette and boomerang DNA amplification." Trends in Biochemical Sciences 20, no. 9 (1995): 372–73. http://dx.doi.org/10.1016/s0968-0004(00)89079-9.

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2

Potter, Christopher J., and Liqun Luo. "Splinkerette PCR for Mapping Transposable Elements in Drosophila." PLoS ONE 5, no. 4 (2010): e10168. http://dx.doi.org/10.1371/journal.pone.0010168.

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3

Horn, Carsten, Jens Hansen, Frank Schnütgen, et al. "Splinkerette PCR for more efficient characterization of gene trap events." Nature Genetics 39, no. 8 (2007): 933–34. http://dx.doi.org/10.1038/ng0807-933.

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4

Horn, Carsten, Jens Hansen, Frank Schnütgen, et al. "Erratum: Splinkerette PCR for more efficient characterization of gene trap events." Nature Genetics 39, no. 12 (2007): 1528. http://dx.doi.org/10.1038/ng1207-1528.

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5

Cavagnaro, Pablo F., Douglas Senalik, and Philipp W. Simon. "SplinkBES: a splinkerette-based method for generating long end sequences from large insert DNA libraries." BioTechniques 47, no. 2 (2009): 681–90. http://dx.doi.org/10.2144/000113122.

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6

Uren, Anthony G., Harald Mikkers, Jaap Kool, et al. "A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites." Nature Protocols 4, no. 5 (2009): 789–98. http://dx.doi.org/10.1038/nprot.2009.64.

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7

Too, W. C. See, Y. C. Liew, and L. L. Few. "Cloning of glyceraldehyde-3-phosphate dehydrogenase from an Antarctic psychrophilic bacterium by inverse and splinkerette PCR." Journal of Basic Microbiology 48, no. 5 (2008): 430–35. http://dx.doi.org/10.1002/jobm.200800008.

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8

Lynn, Elizabeth C., and Robert B. Beckstead. "Identification of Gene Expression Elements in Histomonas meleagridis Using Splinkerette PCR, a Variation of Ligated Adaptor PCR." Journal of Parasitology 98, no. 1 (2012): 135–41. http://dx.doi.org/10.1645/ge-2916.1.

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9

See Too, W. C., and L. L. Few. "Cloning of triose phosphate isomerase gene from an antarctic psychrophilic Pseudomonas sp. by degenerate and splinkerette PCR." World Journal of Microbiology and Biotechnology 26, no. 7 (2010): 1251–59. http://dx.doi.org/10.1007/s11274-009-0295-9.

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10

Cleveland, Susan M., and Utpal P. Dave. "Insertional Activation of GLI2 in Adult T-Cell Leukemia/Lymphoma." Blood 110, no. 11 (2007): 4149. http://dx.doi.org/10.1182/blood.v110.11.4149.4149.

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Abstract Retroviruses induce cancer by integrating into the cellular genome and activating oncogenes or inactivating tumor suppressor genes. Human T-cell Leukemia Virus type 1 (HTLV-1), a complex retrovirus, induces Adult T-cell Leukemia/Lymphoma (ATLL) after a latency of over 30 years and in only 5% of carriers. The long latency and incomplete penetrance is similar to how slow transforming retroviruses induce cancer in mice and imply multiple oncogenic “hits” need to accumulate for clinically apparent disease. Insertional mutagenesis may be one mechanism by which ATLL develops. We used splink

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11

Fili, A. E., A. P. Alessio, W. Garrels, et al. "242 HIGHLY EFFICIENT SLEEPING BEAUTY TRANSPOSON-MEDIATED TRANSGENESIS IN BOVINE FETAL FIBROBLASTS." Reproduction, Fertility and Development 28, no. 2 (2016): 253. http://dx.doi.org/10.1071/rdv28n2ab242.

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Active transposon-mediated transgenesis is an emerging tool for basic and applied research in livestock. We have demonstrated the effectiveness of a helper-independent piggyBac transposon (pGENIE-3) for gene transfer into the genome of bovine cells (Alessio et al. 2014 Reprod. Domest. Anim. 49, 8). Here, we extend our previous research by examining the suitability of a Sleeping Beauty (SB) transposon-based methodology to deliver transgenes into the genome of bovine fetal fibroblasts (BFF), and the ability of these cells to support in vitro embryo development upon somatic cell nuclear transfer

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12

Shen, Dan, Songlei Xue, Shuheng Chan, et al. "Enhancer Trapping and Annotation in Zebrafish Mediated with Sleeping Beauty, piggyBac and Tol2 Transposons." Genes 9, no. 12 (2018): 630. http://dx.doi.org/10.3390/genes9120630.

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Although transposon-mediated enhancer trapping (ET) is successfully applied in diverse models, the efficiency of various transposon systems varies significantly, and little information is available regarding efficiency of enhancer trapping by various transposons in zebrafish. Most potential enhancers (Ens) still lack evidence of actual En activity. Here, we compared the differences in ET efficiency between sleeping beauty (SB), piggyBac (PB) and Tol2 transposons. Tol2 represented the highest germline transfer efficiencies at 55.56% (NF0 = 165), followed by SB (38.36%, NF0 = 151) and PB (32.65%

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13

Sato, Masahiro, Emi Inada, Issei Saitoh, Shingo Nakamura, and Satoshi Watanabe. "In Vivo Piggybac-Based Gene Delivery towards Murine Pancreatic Parenchyma Confers Sustained Expression of Gene of Interest." International Journal of Molecular Sciences 20, no. 13 (2019): 3116. http://dx.doi.org/10.3390/ijms20133116.

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The pancreas is a glandular organ that functions in the digestive system and endocrine system of vertebrates. The most common disorders involving the pancreas are diabetes, pancreatitis, and pancreatic cancer. In vivo gene delivery targeting the pancreas is important for preventing or curing such diseases and for exploring the biological function of genes involved in the pathogenesis of these diseases. Our previous experiments demonstrated that adult murine pancreatic cells can be efficiently transfected by exogenous plasmid DNA following intraparenchymal injection and subsequent in vivo elect

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14

Jia, Wenzhu, Zhongxia Guan, ShaSha Shi, et al. "The Annotation of Zebrafish Enhancer Trap Lines Generated with PB Transposon." Current Issues in Molecular Biology 44, no. 6 (2022): 2614–21. http://dx.doi.org/10.3390/cimb44060178.

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An enhancer trap (ET) mediated by a transposon is an effective method for functional gene research. Here, an ET system based on a PB transposon that carries a mini Krt4 promoter (the keratin4 minimal promoter from zebrafish) and the green fluorescent protein gene (GFP) has been used to produce zebrafish ET lines. One enhancer trap line with eye-specific expression GFP named EYE was used to identify the trapped enhancers and genes. Firstly, GFP showed a temporal and spatial expression pattern with whole-embryo expression at 6, 12, and 24 hpf stages and eye-specific expression from 2 to 7 dpf. T

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15

Salnikov, P. A., A. A. Khabarova, G. S. Koksharova, et al. "Here and there: the double-side transgene localization." Vavilov Journal of Genetics and Breeding 25, no. 6 (2021): 607–12. http://dx.doi.org/10.18699/vj21.068.

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Random transgene integration is a powerful tool for developing new genome-wide screening approaches. These techniques have already been used for functional gene annotation by transposon-insertion sequencing, for identification of transcription factor binding sites and regulatory sequences, and for dissecting chromatin position effects. Precise localization of transgenes and accurate artifact filtration are essential for this type of method. To date, many mapping assays have been developed, including Inverse-PCR, TLA, LAM-PCR, and splinkerette PCR. However, none of them is able to ensure locali

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16

Hasemann, Marie S., Annette B. Sørensen, Finn S. Pedersen, Claus Nerlov, and Bo Porse. "Retroviral Insertional Mutagenesis Screen in a C/EBPalpha Proliferative Genetic Background." Blood 108, no. 11 (2006): 4342. http://dx.doi.org/10.1182/blood.v108.11.4342.4342.

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Abstract The CCAAT enhancer binding protein alpha (C/EBPalpha) transcription factor plays a key role in the regulation of growth and differentiation of the granulocytic lineage in the hematopoietic system. Consistently, mice lacking C/EBPalpha have no mature neutrophils and die within a few hours after birth. In contrast, homozygous knockin mice in which the wild type Cebpa allele has been replaced with a mutant allele (BRM2) deficient in repressing the activity of E2F family members, are viable. At 8 weeks of age these animals display myeloid dysplasia with absence of neutrophil granulocytes.

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17

Qureshi, Safia J., David J. Porteous, and Anthony J. Brookes. "Alu-based vectorettes and splinkerettes." Genetic Analysis: Biomolecular Engineering 11, no. 4 (1994): 95–101. http://dx.doi.org/10.1016/1050-3862(94)90046-9.

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18

Li, Zhe, Jan-Henning Klusmann, Frank J. Godinho, Hee-Won Lee, Dirk Reinhardt, and Stuart H. Orkin. "A Genome-Wide Retroviral Insertional Mutagenesis Screen for Genes Cooperating with Truncated, Oncogenic GATA1s." Blood 106, no. 11 (2005): 2990. http://dx.doi.org/10.1182/blood.v106.11.2990.2990.

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Abstract Somatic mutations in the hematopoietic transcription factor GATA1 are found in megakaryoblasts of Down Syndrome (DS) patients with transient myeloproliferative disorder (TMD, or transient leukemia or TL) and the related acute megakaryoblastic leukemia (DS-AMKL, or DS- AML M7). These mutations lead to production of a GATA1 variant (GATA1s) lacking its N-terminal domain. Mice carrying GATA1s mutation have normal adult hematopoiesis. However, during embryonic/fetal development, we have identified a transient population of abnormal yolk sac/fetal liver megakaryocytic progenitors in mutant

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19

Devon, Rebecca S., David J. Porteous, and Anthony J. Brookes. "Splinkerettes—improved vectorettes for greater efficiency in PCR walking." Nucleic Acids Research 23, no. 9 (1995): 1644–45. http://dx.doi.org/10.1093/nar/23.9.1644.

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20

Tolar, Jakub, Mark Osborn, Scott Bell, et al. "Transgenesis of Multipotent Adult Progenitor Cells (MAPC) with Sleeping Beauty Transposons to Determine MAPC Homing and Persistence in Real-Time In Vivo." Blood 104, no. 11 (2004): 2099. http://dx.doi.org/10.1182/blood.v104.11.2099.2099.

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Abstract MAPC are non-hematopoietic stem cells with the capacity to form most, if not all, cell types of the body. To date, the observations of homing of the MAPC have been limited to post mortem analyses. As MAPC may be useful in cellular therapies, our goal was to map their biodistribution in live organisms. To determine the real-time organ-specific homing pattern of donor MAPC, MAPC (from BM of C57BL/6J-rosa26 mice) were co-nucleofected with cDNAs encoding the red fluorescent protein DsRed2 and luciferase, using the Sleeping Beauty (SB) transposon system. Non-viral gene transfer mediated by

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21

Stoddart, Angela, Anthony Fernald, Rachel Joy Bergerson, et al. "Retroviral Insertional Mutagenesis In Egr1+/- mice, Haploinsufficient For a Human Del(5q) Myeloid Leukemia Gene, Develop Myeloid Neoplasms With Proviral Insertions In Genes Syntenic To Human 5q." Blood 122, no. 21 (2013): 1275. http://dx.doi.org/10.1182/blood.v122.21.1275.1275.

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Abstract Therapy-related myeloid neoplasm (t-MN) is a distinctive clinical syndrome occurring after treatment with chemotherapy and/or radiotherapy, typically for a primary malignant disease. Loss of the long arm of chromosome 5, del(5q), is the most common recurring cytogenetic abnormality and is observed in 40% of t-MN patients, as well as 10-15% of patients with primary MDS or AML de novo. These deletions typically encompass over 70Mb [spanning 5q14-q33] and numerous genes, making the identification of relevant del(5q) genes very challenging. In previous studies, we identified a commonly de

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22

Shao, Hongguang, and James Lok. "Detection of piggyBac-mediated Transposition by Splinkerette PCR in Transgenic Lines of Strongyloides ratti." BIO-PROTOCOL 4, no. 1 (2014). http://dx.doi.org/10.21769/bioprotoc.1015.

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23

Han, Hye-Jin, Dae Hoon Kim, and Jong Youn Baik. "A splinkerette PCR-based genome walking technique for the identification of transgene integration sites in CHO cells." Journal of Biotechnology, May 2023. http://dx.doi.org/10.1016/j.jbiotec.2023.05.007.

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24

Mishra, Priya, Velmurugan Balaraman, and Malcolm J. Fraser. "Maxizyme-mediated suppression of chikungunya virus replication and transmission in transgenic Aedes aegypti mosquitoes." Frontiers in Microbiology 14 (December 22, 2023). http://dx.doi.org/10.3389/fmicb.2023.1286519.

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Chikungunya virus (CHIKV) is an emerging mosquito-borne pathogen of significant public health importance. There are currently no prophylactic vaccines or therapeutics available to control CHIKV. One approach to arbovirus control that has been proposed is the replacement of transmission-competent mosquitoes with those that are refractory to virus infection. Several transgene effectors are being examined as potentially useful for this population replacement approach. We previously demonstrated the successful use of hammerhead ribozymes (hRzs) as an antiviral effector transgene to control CHIKV i

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