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Journal articles on the topic 'Spot-on lawn assay'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Esra, Demir, and Başbülbül Gamze. "Screening of Bacteriocin Production in Lactic Acid Bacteria Isolated From Fermented Dairy Products." Biotechnology Journal International 18, no. 2 (2017): 1–9. https://doi.org/10.9734/BJI/2017/33504.

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<strong>Aims: </strong>In this study we aimed to screen LAB strains from different fermented dairy products by phenotypic and genotypic methods. <strong>Methodology:</strong> 138 LAB isolates belonging to <em>Enterococcus</em> (13,76%), <em>Lactococcus</em> (18,84%) and <em>Lactobacillus</em> (67,39%) genera from fermented dairy products were used to search for their bacteriocin production and bacteriocin encoding genes were searched. LAB isolates were screened by spot-on lawn assay method against indicator microorganisms. <em>Geobacillus stearothermophilus</em> DSMZ 22, <em>Escherichia coli</

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Zwarycz, Allison S., and David E. Whitworth. "Myxobacterial Predation: A Standardised Lawn Predation Assay Highlights Strains with Unusually Efficient Predatory Activity." Microorganisms 11, no. 2 (2023): 398. http://dx.doi.org/10.3390/microorganisms11020398.

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Myxobacteria prey upon a broad range of microorganisms. Lawn assays are commonly used to quantify myxobacterial predation—myxobacterial suspensions are spotted onto prey lawns, and monitored via spot expansion. The diversity in motility behaviours of myxobacterial strains and differing assay protocols in myxobacteriology laboratories led us to develop a highly-specified assay, which was applied to 28 myxobacterial strains preying on seven phytopathogenic prey species. Generally, prey organisms showed no qualitative differences in their susceptibility/resistance to myxobacterial predation. For

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Poimenidou, Sofia V., Athina Skarveli, Georgia Saxami, Evdokia K. Mitsou, Maria Kotsou, and Adamantini Kyriacou. "Inhibition of Listeria monocytogenes Growth, Adherence and Invasion in Caco-2 Cells by Potential Probiotic Lactic Acid Bacteria Isolated from Fecal Samples of Healthy Neonates." Microorganisms 11, no. 2 (2023): 363. http://dx.doi.org/10.3390/microorganisms11020363.

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Lactic acid bacteria (LAB) isolated from healthy humans may prove an effective tool against pathogen growth, adherence and invasion in intestinal epithelial cells. This study aimed to evaluate the antilisterial properties of LAB isolated from fecal samples of healthy neonates. Forty-five LAB strains were tested for their antimicrobial activity against ten Listeria monocytogenes strains with spot-on-lawn and agar-well diffusion assays, and ten lactobacilli strains were further assessed for their inhibitory effect against adherence and invasion of Caco-2 cells by L. monocytogenes EGDe. Inhibitio

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Aruta, Kathleen Joy C., and Christopher Marlowe A. Caipang. "Local aquatic microflora as a potential source of probionts in biofloc technology for whiteleg shrimp, <i>Penaeus vannamei</i>." Environmental and Experimental Biology 23, no. 1 (2025): 1–8. https://doi.org/10.22364/eeb.23.01.

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The Philippine shrimp aquaculture industry, a key supplier of Penaeus vannamei to domestic and international markets, faces significant disease challenges, particularly from pathogenic Vibrio species. Current disease management strategies often employ terrestrially-sourced probiotics, whose efficacy can be limited by environmental incompatibility with the aquatic host. This study aimed to address this limitation by isolating and characterizing putative probiotic microorganisms from the local aquatic environment of P. vannamei. Water samples, collected from a shrimp farm outlet pond in Negros O

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Mussi, Jamili Maria Suhet, Guilherme Campos Tavares, Ethiene Luiza de Souza Santos, et al. "Antagonistic activity of lactic acid bacteria isolated from Minas artisanal cheeses against Brucella abortus." Brazilian Journal of Veterinary Research and Animal Science 60 (August 21, 2023): e204539. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2023.204539.

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This study aimed to evaluate methods for studying the in vitro antimicrobial activity of lactic acid bacteria (LAB) against Brucella abortus and to evaluate the antagonistic effect of LAB on the viability of this pathogen. A total of 18 LAB strains (Lactobacillus plantarum, n = 11; Pediococcus acidilactici, n = 1; Lactobacillus rhamnosus, n = 4; and Lactobacillus brevis, n = 2), isolated from Minas artisanal cheeses produced in three regions (Canastra, Campos das Vertentes, and Araxá) of Minas Gerais State, Brazil, were tested for their antimicrobial activity against B. abortus using three met

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Ali, Zainab. "Screening of marcescin producing isolates from Serratia marcescens." Academic Science Journal 2, no. 4 (2024): 207–16. http://dx.doi.org/10.24237/asj.02.04.811d.

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The current study intends to investigate the production of bacteriocin from S. marcescens, a Gram-negative bacterium, is a human pathogen and the reason why hospital medical equipment becomes contaminated. Various samples from patients about 220 samples which include blood,urine,abscess and sputum, twenty -three isolates of S.marcescens was performed as per standard laboratory procedure and confirmed by using VITEK-2 systems. The isolates of S.marcescens which produced marcescin detected by four methods : spot –on-the –lawn method , Cup-assay method ,Well-diffusion method and Agar cross streak

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Seccareccia, Ivana, Christian Kost, and Markus Nett. "Quantitative Analysis of Lysobacter Predation." Applied and Environmental Microbiology 81, no. 20 (2015): 7098–105. http://dx.doi.org/10.1128/aem.01781-15.

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ABSTRACTBacteria of the genusLysobacterare considered to be facultative predators that use a feeding strategy similar to that of myxobacteria. Experimental data supporting this assumption, however, are scarce. Therefore, the predatory activities of threeLysobacterspecies were tested in the prey spot plate assay and in the lawn predation assay, which are commonly used to analyze myxobacterial predation. Surprisingly, only one of the testedLysobacterspecies showed predatory behavior in the two assays. This result suggested that not allLysobacterstrains are predatory or, alternatively, that the a

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Venegas-Ortega, María Georgina, Adriana Carolina Flores-Gallegos, Cristóbal Noé Aguilar, Raúl Rodríguez-Herrera, José Luis Martínez-Hernández, and Guadalupe Virginia Nevárez-Moorillón. "Multi-Functional Potential of Presumptive Lactic Acid Bacteria Isolated from Chihuahua Cheese." Foods 9, no. 3 (2020): 276. http://dx.doi.org/10.3390/foods9030276.

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The multifunctional properties of autochthonous lactic acid bacteria can be of use for enhancing the sensorial properties of food, as well as in food preservation. An initial screening for antimicrobial, proteolytic, and lipolytic capacities was done in 214 presumptive lactic acid bacteria isolates obtained from Chihuahua cheese manufacturing and during a ripening period of nine months. The antimicrobial screening was done by spot-on-the-lawn tests, using Listeria monocytogenes and Escherichia coli as indicator microorganisms; proteolysis was tested in casein-peptone agar and lipolysis in Mann

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9

Wan, Allison, Cherry Ibarra-Romero, John Vederas, and Sorina Chiorean. "Learning the Science Behind Bacteriocins Through Lacticin 3147; a Promising Lantibiotic." Alberta Academic Review 2, no. 2 (2019): 79–80. http://dx.doi.org/10.29173/aar75.

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As microorganisms continue to develop resistance and survive against many different forms of antimicrobial solutions, such as antibiotics, the threat that antimicrobial resistance poses grows considerably. One solution to this persistent issue could be bacteriocins: ribosomally synthesized antimicrobial peptides that are synthesized by bacteria. In this study, the specific lantibiotic—a subclass of bacteriocin—used was lacticin 3147, which is comprised of two components: A1 and A2. Lacticin 3147 was first purified and isolated in order to properly analyze its antimicrobial effects, which show

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Rozoy, Elodie, Laurent Bazinet, Monica Araya-Farias, Anthony Guernec, and Linda Saucier. "Inhibitory Effects of Commercial and Enriched Green Tea Extracts on the Growth of Brochothrix thermosphacta, Pseudomonas putida and Escherichia coli." Journal of Food Research 2, no. 1 (2012): 1. http://dx.doi.org/10.5539/jfr.v2n1p1.

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<p>The major catechin found in green tea, called epigallocatechin gallate (EGCG), have been reported to have antimicrobial properties. In this study, we examined <em>in vitro </em>the antimicrobial effects of a commercial green tea extract sold in a capsule form, and two prepared green tea extracts enriched in catechins against <em>Brochothrix thermosphacta</em>, <em>Pseudomonsas putida</em> and <em>Escherichia coli</em> which have been associated with meat spoilage. The antimicrobial activity of the different tea extracts was evaluated by

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Nguyen, H., F. Elegado, N. Librojo-Basilio, R. Mabesa, and E. Dizon. "Isolation and characterisation of selected lactic acid bacteria for improved processing of Nem chua, a traditional fermented meat from Vietnam." Beneficial Microbes 1, no. 1 (2010): 67–74. http://dx.doi.org/10.3920/bm2009.0001.

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Nem chua is a traditional lactic acid fermented meat of Vietnam that is consumed raw. During fermentation, the number of acid-producing bacteria increased significantly and by the final day (4th), it had reached approximately 8.4 log cfu/g, contributing to 94% of the total microbiota of the product. Out of 85 isolates obtained from the product, only 44 were confirmed Gram-positive and catalase-negative. These putative LAB isolates were purified and screened for antimicrobial and proteolytic activity. None of the isolates showed bacteriocin activity using the 'spot-on lawn' method, but showed a

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12

Silva, J. G., R. D. Castro, F. M. Sant’Anna, et al. "In vitro assessment of the probiotic potential of lactobacilli isolated from Minas artisanal cheese produced in the Araxá region, Minas Gerais state, Brazil." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 71, no. 2 (2019): 647–57. http://dx.doi.org/10.1590/1678-4162-10188.

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ABSTRACT Minas artisanal cheese is made from endogenous starter cultures, including lactic acid bacteria (LAB). Some LAB may possess probiotic potential. Thus, this study aimed to evaluate the in vitro probiotic properties of lactobacilli isolated from Minas artisanal cheeses produced in Minas Gerais. Ten samples of lactobacilli, formerly isolated from those cheeses, were submitted to the following assays: antimicrobial susceptibility, tolerance to artificial gastric juice and biliary salts, production of hydrogen peroxide and antagonism against pathogenic and non-pathogenic micro-organisms. O

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Knysh, O. V., A. V. Martynov, and T. P. Osolodchenko. "Inhibitory potential of probiotic spore formers grown as mono- and mix-cultures under conditions of different nutrient availability." Modern medical technology 16, no. 4 (2024): 310–17. https://doi.org/10.14739/mmt.2024.4.311797.

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In many clinical cases, the use of probiotic microorganisms is a safer alternative to antibiotic therapy. Spore-forming probiotics, due to their high resistance to damaging environmental factors, stand out among other beneficial microorganisms and, therefore, deserve special attention. Determining the conditions that enhance the inhibitory potential of probiotic bacilli is an important step towards increasing their effectiveness against pathogenic bacteria and pathobionts. The aim of this study was to determine whether the inhibitory potential of spore-forming probiotic species: Alkalihalobaci

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14

Rahaman, MT, M. Rahman, MB Rahman, et al. "Poultry Salmonella Specific Bacteriophage Isolation and Characterization." Bangladesh Journal of Veterinary Medicine 12, no. 2 (2014): 107–14. http://dx.doi.org/10.3329/bjvm.v12i2.21264.

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Bacteriophage named as SAL-PG (Salmonella bacteriophage), specific to Salmonella pullorum and Salmonella gallinarum was isolated by an enrichment method directly from waste water near to poultry farm. For the isolation of phage 4.5 ml water sample, 0.5 ml of 10X Nutrient Broth and 0.5 ml of log phage bacteria were mixed well and incubated at 37° C for overnight followed by centrifugation at 10,000 rpm for 10 minutes. The presence of bacteriophage was observed by spot test over the bacterial lawn of and plaque assay. The host range of the isolated bacteriophage was determined by spot test using

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Li, Songbo, Arishabhas Tantibhadrasapa, Songphon Buddhasiri, et al. "Probiotic, Paraprobiotic, and Postbiotic Activities of Lactiplantibacillus plantarum KUNN19-2 Against Non-Typhoidal Salmonella Serovars." International Journal of Molecular Sciences 26, no. 5 (2025): 1821. https://doi.org/10.3390/ijms26051821.

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Non-typhoidal salmonellosis (NTS) caused by multidrug-resistant (MDR) Salmonella enterica is a significant public health concern worldwide. Probiotics offer a potential alternative to antibiotics in many infectious diseases, including NTS. However, using living bacteria raises safety concerns in clinical settings, especially in the immunocompromised host. This study compared the anti-Salmonella and immunomodulatory effects between viable (probiotics) and heat-killed (paraprobiotics) lactic acid bacteria Lactiplantibacillus plantarum KUNN19-2 (KUNN19-2), isolated from Thai-style fermented pork

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GROWER, JENNIFER L., KAY COOKSEY, and KELLY J. K. GETTY. "Development and Characterization of an Antimicrobial Packaging Film Coating Containing Nisin for Inhibition of Listeria monocytogenes." Journal of Food Protection 67, no. 3 (2004): 475–79. http://dx.doi.org/10.4315/0362-028x-67.3.475.

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The purpose of this study was to develop and characterize a packaging film coating containing nisin. A spot-on-lawn assay was used to determine the effect of acid type (ascorbic, acetic, hydrochloric, lactic) and nisin level (equal increments from 10,000 IU to 9 IU) to be used in the formulation of the film coating. Zones of inhibition were measured after incubation on tryptic soy agar (37°C, 48 h). Low-density polyethylene films coated with differing levels of nisin were characterized by field emission scanning electron microscopy, tensile strength, elongation, and water vapor transmission ra

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Magalhães Júnior, Géssica Aparecida de, Romário Costa Fochat, Humberto Moreira Húngaro, et al. "Partial characterization of a bacteriocin-like inhibitory substance produced by Streptococcus equinus C6I9, a bovine rumen isolate." Concilium 23, no. 15 (2023): 78–96. http://dx.doi.org/10.53660/clm-1661-23i24.

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The present study aimed to evaluate the production and the factors that influence the antimicrobial activity of a bacteriocin-like inhibitory substance (BLIS) produced by Streptococcus equinus C6I9, a bovine rumen isolate. The antagonist activity was evaluated by the spot-on-lawn assay. Antimicrobial activity of the crude extracts obtained using NaCl acidic solution (pH 2, 100 mM) was evaluated along the bacterial growth and in growth medium containing different carbon and nitrogen sources, by agar diffusion method. Crude extract was evaluated regarding temperature stability, storage condition

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Kizheva, Yoana, Zoltan Urshev, Melani Dimitrova, Nevena Bogatzevska, Penka Moncheva, and Petya Hristova. "Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential." Plants 12, no. 4 (2023): 947. http://dx.doi.org/10.3390/plants12040947.

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Bacteriophages have greatly engaged the attention of scientists worldwide due to the continuously increasing resistance of phytopathogenic bacteria to commercially used chemical pesticides. However, the knowledge regarding phages is still very insufficient and must be continuously expanded. This paper presents the results of the isolation, characterization, and evaluation of the potential of 11 phage isolates as natural predators of a severe phytopathogenic bacterium—Xanthomonas euvesicatoria. Phages were isolated from the rhizosphere of tomato plants with symptoms of bacterial spot. The plaqu

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Akomoneh, Elvis Achondou, Zina Gestels, Saïd Abdellati, et al. "Genome Mining Uncovers NRPS and PKS Clusters in Rothia dentocariosa with Inhibitory Activity against Neisseria Species." Antibiotics 12, no. 11 (2023): 1592. http://dx.doi.org/10.3390/antibiotics12111592.

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The growing global threat of antimicrobial resistance is reaching a crisis point as common bacterial infections, including those caused by pathogenic Neisseria species, are becoming increasingly untreatable. This is compelling the scientific community to search for new antimicrobial agents, taking advantage of computational mining and using whole genome sequences to discover natural products from the human microbiome with antibiotic effects. In this study, we investigated the crude extract from a Rothia dentocariosa strain with demonstrated antimicrobial activity against pathogenic Neisseria s

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Fraberger, Vera, Claudia Ammer, and Konrad J. Domig. "Functional Properties and Sustainability Improvement of Sourdough Bread by Lactic Acid Bacteria." Microorganisms 8, no. 12 (2020): 1895. http://dx.doi.org/10.3390/microorganisms8121895.

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Preventing food spoilage without the addition of chemical food additives, while increasing functional properties of wheat-based bakery products, is an increasing demand by the consumers and a challenge for the food industry. Within this study, lactic acid bacteria (LAB) isolated from sourdough were screened in vitro for the ability to utilize the typical wheat carbohydrates, for their antimicrobial and functional properties. The dual culture overlay assay revealed varying levels of inhibition against the examined fungi, with Lactiplantibacillus plantarum S4.2 and Lentilactobacillusparabuchneri

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Zhang, Peipei, József Baranyi, and Mark Tamplin. "Interstrain Interactions between Bacteria Isolated from Vacuum-Packaged Refrigerated Beef." Applied and Environmental Microbiology 81, no. 8 (2015): 2753–61. http://dx.doi.org/10.1128/aem.03933-14.

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ABSTRACTThe formation of bacterial spoilage communities in food is influenced by both extrinsic and intrinsic environmental factors. Although many reports describe how these factors affect bacterial growth, much less is known about interactions among bacteria, which may influence community structure. This study investigated interactions among representative species of bacteria isolated from vacuum-packaged (VP) beef. Thirty-nine effectors and 20 target isolates were selected, representing 10 bacterial genera:Carnobacterium,Pseudomonas,Hafnia,Serratia,Yersinia,Rahnella,Brochothrix,Bacillus,Leuc

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Knysh, O. V., A. V. Martynov, S. I. Pokhyl, and N. I. Skliar. "The behavior of the Bacillus probiotic species under conditions of co-cultivation." Modern medical technology, no. 4 (December 7, 2023): 59–69. http://dx.doi.org/10.34287/mmt.4(59).2023.8.

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Combining probiotic bacteria is a promising strategy to increase the effectiveness and avoid side effects of probiotic therapy. Bacteria that find themselves in a common environment are able to both change their properties and show new ones under the influence of each other. The change of colonization and antagonistic properties, which provide bacteria with competitive advantages in the development of new spaces, deserves special attention. The aim of this research was to study the features of the mutual influence of probiotic bacilli: B. clausii, B. coagulans and B. subtilis on growth,

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Li, Jieling, Ming Hu, Yang Xue, et al. "Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae." Microorganisms 8, no. 5 (2020): 697. http://dx.doi.org/10.3390/microorganisms8050697.

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Dickeya zeae is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by D. zeae. The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed

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JANES, M. E., R. NANNAPANENI, and M. G. JOHNSON. "Identification and Characterization of Two Bacteriocin-Producing Bacteria Isolated from Garlic and Ginger Root†." Journal of Food Protection 62, no. 8 (1999): 899–904. http://dx.doi.org/10.4315/0362-028x-62.8.899.

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Two bacteriocin-producing bacterial strains were isolated from garlic and ginger root by the agar overlay method. The bacteria were identified by 16S rRNA sequence analyses and fermentation patterns as Leuconostoc mesenteroides (garlic isolate) and Lactococcus lactis (ginger isolate). The bacteriocins were assigned the names leucocin BC2 and lactocin GI3, respectively. Physiochemical properties and antimicrobial spectra of the bacteriocins were determined by the spot-on-lawn method. Both bacteriocins were inhibited by proteolytic enzymes. Leucocin BC2 exhibited a narrow antimicrobial spectrum,

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KIM, MI-HEE, YOON-JUNG KONG, HONG BAEK, and HYUNG-HWAN HYUN. "Production, Purification, and Characterization of Micrococcin GO5, a Bacteriocin Produced by Micrococcus sp. GO5 Isolated from Kimchi." Journal of Food Protection 68, no. 1 (2005): 157–63. http://dx.doi.org/10.4315/0362-028x-68.1.157.

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Strain GO5, a bacteriocin-producing bacterium, was isolated from green onion kimchi and identified as Micrococcus sp. The bacteriocin, micrococcin GO5, displayed a broad spectrum of inhibitory activity against a variety of pathogenic and nonpathogenic microorganisms, as tested by the spot-on-lawn method; its activity spectrum was almost identical to that of nisin. Micrococcin GO5 was inactivated by trypsin (whereas nisin was not) and was completely stable at 100°C for 30 min and in the pH range of 2.0 to 7.0. Micrococcin GO5 exhibited a typical mode of bactericidal activity against Micrococcus

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Quintanilla-Pineda, Mario, Chajira Garrote Achou, Jesús Díaz, et al. "In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida." Foods 12, no. 4 (2023): 861. http://dx.doi.org/10.3390/foods12040861.

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The use of antibiotics in aquaculture leads to the proliferation of multidrug-resistant bacteria, and an urgent need for developing new alternatives to prevent and control disease has, thus, arisen. In this scenario, postbiotics represent a promising tool to achieve this purpose; thus, in this study, isolation and selection of bacteria to further produce and evaluate their postbiotics antibacterial activity against fish pathogens was executed. In this respect, bacterial isolates from rainbow trout and Nile tilapia were obtained and tested in vitro against Yersinia ruckeri and Aeromonas salmoni

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LITT, PUSHPINDER KAUR, JOYJIT SAHA, and DIVYA JARONI. "Characterization of Bacteriophages Targeting Non-O157 Shiga Toxigenic Escherichia coli." Journal of Food Protection 81, no. 5 (2018): 785–94. http://dx.doi.org/10.4315/0362-028x.jfp-17-460.

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ABSTRACT Non-O157 Shiga toxigenic Escherichia coli (STEC) are an important group of foodborne pathogens, implicated in several outbreaks and recalls in the past 2 decades. It is therefore crucial to devise effective control strategies against these pathogens. Bacteriophages present an attractive alternative to conventional pathogen control methods in the food industry. Bacteriophages, targeting non-O157 STEC (O26, O45, O103, O111, O121, O145), were isolated from beef cattle operations in Oklahoma. Their host range and lytic ability were determined against several (n = 21) non-O157 STEC isolate

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MURINDA, SHELTON E., ROBERT F. ROBERTS, and STEPHANIE DOORES. "Evaluation of Lactic Acid–Producing Bacillus and Sporolactobacillus for Antilisterial Activity." Journal of Food Protection 58, no. 5 (1995): 570–72. http://dx.doi.org/10.4315/0362-028x-58.5.570.

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Fifty-five strains of Bacillus (53 B. laevolacticus and 2 B. racemilacticus) and 31 strains of Sporolactobacillus (3 S. inulinus, 19 S. laevus, and 9 S. racemicus) were screened for specific inhibitory activity against 8 strains of Listeria monocytogenes. Putative producer strains were propagated in Eugon, lactobacillus MRS, or sporolactobacillus broths (48–96 h, 37°C, 9.4% CO2), Assays to detect inhibition of Listeria monocytogenes were carried out on Eugon or Bacto-Agar at 37°C (9.4% CO2). Inhibition was absent in direct assays using spot-on-the-lawn techniques but was found in well diffusio

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Leonora, Francis Harry Shone V., and Christopher Marlowe A. Caipang. "Isolation and characterization of gill mucus-associated antagonistic bacteria in the Asian swamp eel (<i>Monopterus albus</i>)." Environmental and Experimental Biology 23, no. 1 (2025): 9–18. https://doi.org/10.22364/eeb.23.02.

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This study investigated the gill mucus of Asian swamp eel (Monopterus albus) for the presence of antagonistic bacteria against known aquatic pathogens, Vibrio harveyi and Aeromonas hydrophila. Initial screening of 500 bacterial isolates via the spot-on-lawn and co-incubation assays, identified five with significant antagonistic activity, which were further subjected to morphological, enzymatic, biochemical, and molecular characterization. All five isolates were Gram-negative bacilli. Four isolates were identified as belonging to the Pseudomonas genus, a known probiont with documented biocontro

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Twomey, Ellen, Paula M. O’Connor, Aidan Coffey, et al. "Inhibition of Clinical MRSA Isolates by Coagulase Negative Staphylococci of Human Origin." Antibiotics 13, no. 4 (2024): 338. http://dx.doi.org/10.3390/antibiotics13040338.

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Staphylococcus aureus is frequently highlighted as a priority for novel drug research due to its pathogenicity and ability to develop antibiotic resistance. Coagulase-negative staphylococci (CoNS) are resident flora of the skin and nares. Previous studies have confirmed their ability to kill and prevent colonization by S. aureus through the production of bioactive substances. This study screened a bank of 37 CoNS for their ability to inhibit the growth of methicillin-resistant S. aureus (MRSA). Deferred antagonism assays, growth curves, and antibiofilm testing performed with the cell-free supe

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Robertson, N. L., and K. L. Brown. "First Report of Bean yellow mosaic virus in Alaska from Clover (Trifolium spp.)." Plant Disease 94, no. 3 (2010): 372. http://dx.doi.org/10.1094/pdis-94-3-0372a.

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In mid-June 2008, distinct mosaic leaves were observed on a cluster of clover (Trifolium spp.) with light pink and white flowers growing at the edge of a lawn in Palmer, AK. Virus minipurification from leaves of affected clover and protein extractions on a polyacrylamide electrophoresis implicated a ~35-kDa putative coat protein (CP). Subsequent western blots and ELISA with a universal potyvirus antiserum (Agdia Inc., Elkhart, IN) confirmed potyvirus identity. Total RNA extracts (RNeasy Plant Mini Kit, Qiagen Inc., Valencia, CA) from the same plant were used for reverse transcription (RT)-PCR.

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Insanu, Muhamad, Ida Maryam, Dadan Rohdiana, and Komar Ruslan Wirasutisna. "UJI AKTIVITAS ANTIBAKTERI LIMA BELAS JENIS MUTU TEH HITAM ORTODOKS ROTORVANE DAN TEH PUTIH (CAMELLIA SINENSIS VAR. ASSAMICA) PADA STAPHYLOCOCCUS AUREUS ATCC 6538." Acta Pharmaceutica Indonesia 42, no. 1 (2017): 32–41. http://dx.doi.org/10.5614/api.v42i1.5385.

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ABSTRAKCamellia sinensis (L.) Kuntze atau teh merupakan tumbuhan yang berasal dari suku theceae, digolongkan menjadi beberapa jenis, yaitu teh hitam, teh oolong, teh hijau, dan teh putih. Secara tradisional teh digunakan sebagai obat kumur hal ini diperkuat oleh beberapa penelitian sebelumnya yang membuktikan aktivitas antibakteri terhadap bakteri yang ada pada rongga mulut antara lain Staphylococcus aureus. Penelitian ini bertujuan untuk membandingkan aktivitas antibakteri dari lima belas mutu teh hitam dan teh putih yang diekstraksi dengan pelarut yang berbeda kepolaran. Serbuk simplisia dau

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Kamath, Rajesh, Helmut Brand, Nisha Nayak, Vani Lakshmi, Reena Verma, and Prajwal Salins. "District-Level Patterns of Health Insurance Coverage and Out-of-Pocket Expenditure on Caesarean Section Deliveries in Public Health Facilities in India." Sustainability 15, no. 5 (2023): 4608. http://dx.doi.org/10.3390/su15054608.

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Reducing catastrophic out-of-pocket expenditure (OOPE) and increasing the rates of institutional deliveries are part of the Sustainable Development Goals (SDGs). India has made significant progress on the maternal and child health front in recent years. India’s National Health Mission (NHM) has been able to increase rates of institutional deliveries. In the present study, we aim to ascertain district-level patterns of percentage of health insurance coverage in the National Family Health Surveys NFHS 4 and NFHS 5. We also aim to ascertain district-level patterns of out-of-pocket expenditure on

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Mills, Emma, Marrisa P. Griffifth, Vatsala Rangachar, et al. "742. Strain Replacement of Vancomycin-Resistant Enterococcus faecium over 6 years at a Large Tertiary Care Center." Open Forum Infectious Diseases 10, Supplement_2 (2023). http://dx.doi.org/10.1093/ofid/ofad500.803.

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Abstract Background E. faecium is a common hospital-acquired bacteria in the United States, with a high prevalence of vancomycin resistance seen within this species. Hospital-adapted vancomycin-resistant E. faecium (VREfm) lineages, such as ST17 and ST117, contribute to the spread and persistence of VREfm within healthcare systems. Although the dynamics of these lineages within healthcare systems have been characterized, little is understood as to what drives strain replacement in VREfm. Methods Comparative genomic analyses were performed on 718 clinical VREfm isolates collected between Januar

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Hitchcock, Nicole M., Danielle Nunes, Josiane Dantas, et al. "2773. Brazilian Bacteriophage Activity Against Multidrug-Resistant (MDR) Klebsiella pneumoniae Isolated from Patients with Severe Nosocomial Infection." Open Forum Infectious Diseases 10, Supplement_2 (2023). http://dx.doi.org/10.1093/ofid/ofad500.2384.

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Abstract Background Phage therapy has re-emerged as a promising strategy for combatting antimicrobial resistance. Phages are ubiquitous viruses that infect and destroy bacteria and are easily isolated from environmental samples. An impressive reservoir of potentially efficacious bacteriophages has yet to be discovered, and the isolation of novel phages from diverse environments is of global importance. Methods A phage research lab was implemented in Salvador Bahia, Brazil, in collaboration with the Center for Innovative Phage Applications and Therapeutics (IPATH) located in San Diego, Californ

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Macaluso, Giusi, Gerlando Fiorenza, Raimondo Gaglio, Isabella Mancuso, and Maria Luisa Scatassa. "In vitro evaluation of bacteriocin-like inhibitory substances produced by lactic acid bacteria isolated during traditional Sicilian cheese making." Italian Journal of Food Safety 5, no. 1 (2016). http://dx.doi.org/10.4081/ijfs.2016.5503.

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Bacteriocins are antimicrobial proteins produced by bacteria that inhibit with a bactericidal or bacteriostatic mode of action, the growth of other bacteria. Many lactic acid bacteria (LAB) produce a high diversity of different bacteriocins. Bacteriocinogenic LAB are generally recognized as safe (GRAS) and useful to control the frequent development of pathogens and spoilage microorganisms; for this reason they are commonly used as starter cultures in food fermentations. In this study, the authors describe the results of a screening on 699 LAB isolated from wooden vat surfaces, raw milk and tra

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Dhar, Sakshi, Sonia Nain, Vaishali Gupta, et al. "P26 Evaluating the antibacterial activity of recombinant phage-encoded lysin against drug-resistant uropathogenic Escherichia coli." JAC-Antimicrobial Resistance 5, Supplement_3 (2023). http://dx.doi.org/10.1093/jacamr/dlad077.030.

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Abstract Background Atrocious use of antibiotics has led to the emergence of MDR in uropathogenic Escherichia coli (UPEC) that poses a serious challenge in the management of urinary tract infections (UTIs). The WHO has described antibiotic resistance in uropathogens as a key pressure point in the burgeoning global antimicrobial resistance crisis. Given this grim situation, we are exploring phage-encoded lysins as plausible alternatives. Objectives Our study involved an in silico strategy for the discovery and characterization of lysin sequences (seq) targeting E. coli cell wall and evaluating

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Daubie, Valéry, Houssein Chalhoub, Bob Blasdel, et al. "Determination of phage susceptibility as a clinical diagnostic tool: A routine perspective." Frontiers in Cellular and Infection Microbiology 12 (September 21, 2022). http://dx.doi.org/10.3389/fcimb.2022.1000721.

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As the global burden of disease caused by multidrug resistant bacteria is a major source of concern, credible clinical alternatives to antibiotic therapy, such as personalized phage therapy, are actively explored. Although phage therapy has been used for more than a century, the issue of an easy to implement diagnostic tool for determining phage susceptibility that meets current routine clinical needs is still open. In this Review, we summarize the existing methods used for determining phage activity on bacteria, including the three reference methods: the spot test, the double agar overlay pla

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Lat, Emil, Francisco Elegado, and Ida Dalmacio. "Isolation and identification of bacteriocin-producing lactic acid bacteria from plants in Mount Makiling Forest Reserve, Philippines." Annals of Tropical Research, April 16, 2019, 1–15. http://dx.doi.org/10.32945/atr4111.2019.

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Sixty-three lactic acid bacteria (LAB) were isolated from trapped or stagnated water in parts of 14 different plant species in Mount Makiling Forest Reserve, Philippines and were screened for bacteriocin production. Thirteen of these isolates were found antagonistic to different indicator bacterial strains through direct assay. However, after screening of the pH neutralized culture supernatant using ‘spot-on-lawn’ method against Enterococcus faecium 79 as the indicator strain, only one isolate, designated as ML 258, was confirmed to be bacteriocin-producer. Its crude extract of bacteriocin exh

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Kassem, Mohamed Ali, Amal Eissa Saafan, Faten Bayomy та Ahmed Osama El-Gendy. "Exploring clinically isolated Staphylococcus sp. bacteriocins revealed the production of amonabactin, micrococcin, and α-circulocin". Iranian Journal of Microbiology, 14 квітня 2021. http://dx.doi.org/10.18502/ijm.v13i2.5983.

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Background and Objectives: Bacteriocins are considered alternative non-conventional antimicrobials produced by certain bacteria with activity against closely related species. The present study focuses on screening, characterization, and partial purification of bacteriocins produced by Staphylococcus sp. isolated from different clinical sources such as pus and blood. Materials and Methods: A total of 100 Staphylococcus isolates were screened for bacteriocin production using spot on lawn assay and agar diffusion method against five indicator bacteria. Bacteriocins from five selected highly

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Zhang, Peipei, Michael Gänzle, and Xianqin Yang. "Complementary Antibacterial Effects of Bacteriocins and Organic Acids as Revealed by Comparative Analysis of Carnobacterium spp. from Meat." Applied and Environmental Microbiology 85, no. 20 (2019). http://dx.doi.org/10.1128/aem.01227-19.

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ABSTRACT Carnobacterium maltaromaticum and Carnobacterium divergens are often predominant in the microbiota of vacuum-packaged (VP) meats after prolonged storage at chiller temperatures, and more so in recent studies. We investigated the antibacterial activities of C. maltaromaticum and C. divergens (n = 31) from VP meats by phenotypic characterization and genomic analysis. Five strains showed antibacterial activities against Gram-positive bacteria in a spot-lawn assay, with C. maltaromaticum strains having an intergeneric and C. divergens strains an intrageneric inhibition spectrum. This inhi

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Hofkens, Nele, Zina Gestels, Said Abdellati, et al. "Microbisporicin (NAI-107) protects Galleria mellonella from infection with Neisseria gonorrhoeae." Microbiology Spectrum, October 12, 2023. http://dx.doi.org/10.1128/spectrum.02825-23.

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ABSTRACT Due to the increasing incidence of antibiotic-resistant bacterial pathogens, the need for the development of alternative compounds is of utmost importance. Bacteriocins are considered a promising alternative to antibiotics. In this study, we determined the in vitro activities of 65 bacteriocins from the PARAGEN collection along with microbisporicin (NAI-107) against 14 WHO reference isolates of Neisseria gonorrhoeae , one isolate of Neisseria meningitidis , and four commensal Neisseria spp. The initial screening of the bacteriocins was carried out using a spot-on-lawn assay wherein 12

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Aladeboyeje, Oluwaseun Temitope, Nazmiye Ozlem Sanli, and Umut Buyuk. "Evaluation of the Antimicrobial Efficacy of some Fermented Traditional Turkish Beverages with Probiotic Potentials." Johnson Matthey Technology Review, 2021. http://dx.doi.org/10.1595/205651322x16388083409013.

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Turkey is a home country for a good number of fermented beverages derived from milk, cereals, fruits and vegetables, and several studies have reported the probiotic potentiality of these beverages. Probiotics, otherwise known as beneficial microorganisms possess the ability to exert antimicrobial effects, which is one of the most important selection criteria for their use in commercial products. In the current study, the antimicrobial activities of potential probiotic bacteria isolated from five fermented traditional Turkish beverages (boza, kefir, ayran, shalgam juice and hardaliye) were eval

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Ghasemian, Abdollah, sima Shakerhagh, and zahra Moradpour. "Evaluation of inhibitory effect of Magnesium Oxide nanoparticles on the viability of planktonic and biofilm forms of Pseudomonas aeruginosa." European Journal of Microbiology and Infectious Diseases, 2025, 1. https://doi.org/10.5455/ejmid.20241105085834.

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The need for developing new antimicrobial approaches comes from the growing antibiotic resistance in pathogenic bacteria, which is a serious concern to public health. Metal nanoparticles have increasingly been investigated in this field owing to their multiple mechanisms of action against microbes. The object of this study was to evaluate the antimicrobial activity of Magnesium Oxide nanoparticles on Pseudomonas aeruginosa. The effect of different concentrations of nanoparticles on both planktonic and biofilm forms was assessed by recording the corresponding optical density. Spot assays showed

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Tantibhadrasapa, Arishabhas, Songbo Li, Songphon Buddhasiri, et al. "Probiotic Limosilactobacillus reuteri KUB-AC5 decreases urothelial cell invasion and enhances macrophage killing of uropathogenic Escherichia coli in vitro study." Frontiers in Cellular and Infection Microbiology 14 (July 18, 2024). http://dx.doi.org/10.3389/fcimb.2024.1401462.

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IntroductionBacterial urinary tract infections (UTI) are among the most common infectious diseases worldwide. The rise of multidrug-resistant (MDR) uropathogenic Escherichia coli (UPEC) UTI cases is a significant threat to healthcare systems. Several probiotic bacteria have been proposed as an alternative to combat MDR UTI. Lactic acid bacteria in the genus Limosilactobacillus are some of the most studied and used probiotics. However, strain-specific effects play a critical role in probiotic properties. L. reuteri KUB-AC5 (AC5), isolated from the chicken gut, confers antimicrobial and immunobi

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Rajeswari, T., U. Vasantha Lakshmi, and B. Vijai Anand Babu. "TAY-SACHS DISEASE IN A CHILD: A RARE CASE REPORT ,GGH KURNOOL." PARIPEX INDIAN JOURNAL OF RESEARCH, March 15, 2023, 53–55. http://dx.doi.org/10.36106/paripex/3808650.

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The neurodegenerative condition known as Tay-Sachs disease is inherited in an autosomal recessive pattern and is caused by an abnormal accumulation of the cell membrane glycolipid and GM2 ganglioside within the lysosomes of affected cells. A lack of the isoenzyme -hexosaminidase A, which is synthesised in the endoplasmic reticulum, is the root cause of this condition. In patients who have Tay-Sachs disease, the first few months of life are characterised by normal motor development, followed by gradual weakening and loss of motor abilities beginning around 2 to 6 months of life. Due to the irre

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Cheng, Yanfei, Hui Zhu, Zhengda Du, et al. "Eukaryotic translation factor eIF5A contributes to acetic acid tolerance in Saccharomyces cerevisiae via transcriptional factor Ume6p." Biotechnology for Biofuels 14, no. 1 (2021). http://dx.doi.org/10.1186/s13068-021-01885-2.

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Abstract Background Saccharomyces cerevisiae is well-known as an ideal model system for basic research and important industrial microorganism for biotechnological applications. Acetic acid is an important growth inhibitor that has deleterious effects on both the growth and fermentation performance of yeast cells. Comprehensive understanding of the mechanisms underlying S. cerevisiae adaptive response to acetic acid is always a focus and indispensable for development of robust industrial strains. eIF5A is a specific translation factor that is especially required for the formation of peptide bon

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sprotocols. "Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes." December 30, 2014. https://doi.org/10.5281/zenodo.13616.

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Authors: Yuri G. Strukov and Andrew S. Belmont This protocol was adapted from “Development of Mammalian Cell Lines with lac Operator-Tagged Chromosomes,” Chapter 25, in[ *Live Cell Imaging* ](http://www.cshlpress.com/link/livecelp.htm)(eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005. ### INTRODUCTION The discovery and use of fluorescent proteins to label chromosomal proteins has yielded basic structural information as well as insights into dynamics that were previously inaccessible. This protocol describes a method for tagging specific chromoso

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