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Högmo, Anders. "Squamous cell carcinomas and preneoplastic lesions of the oral cavity : biological factors and prognosis /." Stockholm, 1999. http://diss.kib.ki.se/1999/91-628-3370-7/.
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Jatana, Courtney Ann. "Histopathological Characteristics in Squamous Cell Carcinoma of the Oral Cavity with Regard to Presence of Circulating Tumor Cells." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1313587893.
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Anand, Sumeet M. 1978. "The correlation between tumour volume and survival in oral cavity and oropharyngeal squamous cell carcinoma /." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111587.
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The Tumour-Node-Metastasis (TNM) classification system of tumour stage does not always reflect the actual tumour mass present at diagnosis. Recent reports propose that volumetric analysis may allow improved stratification of disease recurrence and survival in head and neck squamous cell cancer (SCC). This study aims to assess the prognostic value of tumour volume on the outcome of patients with oral cavity and oropharyngeal SCC.A retrospective review of 73 patients was completed. Tumours were outlined semi-automatically in digitized computed tomography scans, and volumes computed based on surface triangulations of three-dimensional reconstructions with novel software developed at McGill.
Results illustrate significant interstage variability within the current TNM model. Moreover, in oral cavity and oropharyngeal SCC, tumour volume as well as T-stage are significant and independent predictors of disease free survival and overall survival.
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Marinho, Erasmo Bernardo. "Evaluation of the quality of life of post- irradiated patients diagnosed with squamous cell carcinoma of the oral cavity." Universidade Federal do CearÃ, 2016. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16162.
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Radiotherapy is an effective therapeutic modality employed in the management of malignant tumors in the oral cavity. Its use in head and neck results in adverse effects. The measurement of quality of life allows to gather the most common problems in oral cancer patients in a structured way, ranking its intensity and enabling a more accurate clinical control. This study aimed to evaluate the quality of life of post- irradiated diagnosed with squamous cell carcinoma of the oral cavity. Eleven patients over six months of completion of therapy treated at the Hospital Haroldo JuaÃaba â Instituto do CÃncer do Cearà were included in the study. University of Washington quality of life questionnaire, specific to head and neck cancer, was applied. Data related to socio-demographic, clinical, pathological and therapeutic profile were collected. Unstimulated salivary flow was measured by spitting method. Categorical data were exposed in the form of absolute and percentage frequency, and quantitative data as mean  standard deviation, followed by their minimum and maximum. Statistical analysis of quantitative data was performed by nonlinear Spearman correlation, considering a 95% confidence. Most patients were elderly (72.72%), male (81.81%), with lower education (90.9%), away from their work activities since the completion of cancer treatment (90.9%), with a history of smoking (90.9%), alcohol abuse (90.9%) and exposure to sunlight before the onset of the disease (90.9%), with lesions in the mouth floor (45.45%) moderately differentiated type (100%), stage IV (54,54%), subjected to other treatment modalities in addition to radiation therapy (100%). Among the domains assessed, chewing, saliva and speech presented the lowest mean scores (31.8, 42.3 and 60.6, respectively). All participants presented moderate to severe hyposalivation. Statistically significant correlation was found between age and speech (p = 0.043); time of completion of radiation therapy and recreation (p = 0.027); swallowing and pain (p = 0.039); activity and recreation (p = 0.030); swallowing and chewing (p = 0.007); chewing and speaking (p = 0.048); and shoulder and mood (p = 0.004). We conclude that the post- irradiated patients diagnosed with squamous cell carcinoma of the oral cavity tend to consider their quality of life ranging from fair to outstanding and that chewing, saliva and speech domains are those with greater commitment.A radioterapia à uma modalidade terapÃutica eficaz utilizada no tratamento de tumores malignos da cavidade oral. Sua utilizaÃÃo em regiÃo de cabeÃa e pescoÃo leva ao surgimento de efeitos adversos. A mensuraÃÃo da qualidade de vida permite reunir os problemas mais comuns aos pacientes com cÃncer oral de forma estruturada, classificando sua intensidade, tornando-se possÃvel um controle clÃnico mais apurado. O presente estudo teve como objetivo avaliar a qualidade de vida de pÃs-irradiados com diagnÃstico de carcinoma espinocelular de cavidade oral. Onze pacientes com mais de seis meses de conclusÃo do tratamento, atendidos no Hospital Haroldo JuaÃaba â Instituto do CÃncer do CearÃ, foram incluÃdos no estudo. Foi aplicado o questionÃrio de qualidade de vida da Universidade de Washington, especÃfico para o cÃncer de cabeÃa e pescoÃo. Foram coletados dados relacionados ao perfil sociodemogrÃfico, clÃnico-patolÃgico e terapÃutico. Realizou-se a mensuraÃÃo do fluxo salivar nÃo estimulado atravÃs do mÃtodo de spitting. Dados categÃricos foram expostos em forma de frequÃncia absoluta e percentual e os dados quantitativos em forma de mÃdia  desvio-padrÃo, seguido de sua mÃnima e mÃxima. A anÃlise estatÃstica dos dados quantitativos foi realizada atravÃs da correlaÃÃo nÃo linear de Spearman, considerando uma confianÃa de 95%. Predominaram pacientes idosos (72,72%), do sexo masculino (81,81%), de baixa escolaridade (90,9%), afastados de suas atividades laborais desde a conclusÃo do tratamento oncolÃgico (90,9%), com histÃrico de tabagismo (90,9%), abuso de Ãlcool (90,9%) e exposiÃÃo à luz solar anterior ao surgimento da doenÃa (90,9%), com lesÃes localizadas em soalho de boca (45,45%) do tipo moderadamente diferenciado (100%), estadiamento IV (54,54%), submetidas a outras modalidades de tratamento alÃm da radioterapia (100%). Dentre os domÃnios avaliados, mastigaÃÃo, saliva e fala apresentaram as menores mÃdias de escores (31,8; 42,3 e 60,6, respectivamente). Todos os participantes apresentaram hipossalivaÃÃo de moderada à severa. Constatou-se correlaÃÃo estatisticamente significante entre: idade e fala (p=0,043); tempo de conclusÃo da radioterapia e recreaÃÃo (p=0,027); dor e deglutiÃÃo (p=0,039); atividade e recreaÃÃo (p=0,030); deglutiÃÃo e mastigaÃÃo (p=0,007); mastigaÃÃo e fala (p=0,048); e ombro e humor (p=0,004). Conclui-se que os pacientes pÃs-irradiados com diagnÃstico de carcinoma espinocelular de cavidade oral tendem a considerar de mÃdia a excelente sua qualidade de vida e que os domÃnios de mastigaÃÃo, saliva e fala sÃo os que apresentam maior comprometimento.
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El-Hakim, Ibrahim El-Sayed M. "Study of the 5-lipoxygenase pathway of arachidonic acid metabolism (leukotrienes) in squamous cell carcinoma of the oral cavity." Thesis, King's College London (University of London), 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.669048.
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Abreu, Priscila Marinho de. "HPV e Expressão de p16 como biomarcadores de prognósticos em carcinoma de células escamosas da cavidade bucal." Universidade Federal do Espírito Santo, 2015. http://repositorio.ufes.br/handle/10/4505.
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Abreu, Priscila Marinho de. "HPV e expressão de p16 como biomarcadores de prognóstico em carcinoma de células escamosas da cavidade bucal." Mestrado em Biotecnologia, 2015. http://repositorio.ufes.br/handle/10/1881.
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Biomarcadores de progressão tumoral são importantes na determinação do prognóstico e resposta ao tratamento em carcinoma de células escamosas da cavidade bucal. Este estudo teve como objetivo avaliar a frequência de infecção pelo HPV e a expressão de p16 como biomarcador de prognóstico em carcinoma de células escamosas da cavidade bucal. Dados clínico-patológicos e tecido tumoral de 90 indivíduos com carcinoma de células escamosas da cavidade bucal foram obtidos por entrevista e análise de prontuários. Detecção de HPV foi realizada à partir de amostras de tecido tumoral por PCR utilizando o conjunto de primers PGMY09/11. A seguir, as mesmas amostras foram submetidas à PCR com os primers MY09/11 e nested PCR com os primers GP5+/6+. Expressão de p16 foi detectada por imunohistoquímica. Análise estatística foi feita através de associação de variáveis, utilizando o teste de qui-quadrado e exato de Fisher. Sobrevida doença-específica (SDE) e sobrevida livre de doença (SLD) foram estimadas usando método de Kaplan-Meier. Comparação entre as curvas de sobrevida foram realizadas com o teste Log-Rank. Comparação entre status da infecção pelo HPV e expressão de p16 foram analisadas pelo teste Cox. Todos os testes com P ≤ 0.05 foram considerados significantes. A frequência de infecção pelo HPV na população estudada foi de 3,70% detectadas apenas com a nested PCR. Expressão de p16 foi observada em 21,87% das amostras analisadas. A SDE foi de 25,5 meses (95% IC = 20,03 - 31,00), enquanto a SLD foi de 30 meses (95% IC = 25,63 – 34,48). Maior SDE foi observada em indivíduos com tumores em estádio inicial (I-II) (P=0,001), tumores < 2cm (P=0,001), sem metástase em linfonodos regionais (P=0,006) e aqueles submetidos a tratamento cirúrgico (P<0,0001). Em conclusão, o status da infecção pelo HPV não mostrou-se um bom marcador de prognóstico e parece não ser determinante na tumorigênese em CCE da cavidade bucal, uma vez que apresentou baixa frequência de infecção. A expressão de p16 não foi um indicador determinante da presença de HPV nas amostras estudadas e um maior número de casos é necessário para avaliar sua aplicabilidade como marcador de prognóstico na população estudada.
Tumor progression biomarkers are important in determining prognosis and treatment response in squamous cell carcinoma of the oral cavity. This study aimed to evaluate the frequency of HPV infection and p16 expression as a biomarker of prognosis in squamous cell carcinoma of the oral cavity. Clinicopathological data and tumor tissue of 90 patients with squamous cell carcinoma of the oral cavity were obtained by interview and review of medical records. HPV detection was performed starting from tumor tissue samples by PCR using the primer set PGMY09/11. Next, the same samples were subjected to PCR with primers MY09 / 11 primers and nested PCR with GP5+/6+ primers. P16 expression was detected by immunohistochemistry. Statistical analysis was performed using variable association, using the chi-square and Fisher exact test. Disease-specific survival (DSS) and disease-free survival (DFS) were estimated using Kaplan-Meier. Comparison between the survival curves were performed using the log-rank test. Comparison status of HPV infection and p16 expression were analyzed by Cox test. All tests with P≤0,05 were considered significant. The frequency of HPV infection in this population was 3,70% detected only with nested PCR. p16 expression was observed in 21.87% of the analyzed samples. The DSS was 25.5 months (95% CI = 20.03 to 31.00), while the DFS was 30 months (95% CI = 25.63 to 34.48). Most DSS was observed in subjects with initial stage tumors (I-II) (P=0,001), tumor <2 cm (P=0,001), without regional lymph node metastasis (P=0,006) and those undergoing surgical treatment (P<0,0001). In conclusion, the status of HPV infection not proved to be a good prognostic marker and does not seem to be decisive in tumorigenesis in SCC of the oral cavity, since it showed low frequency of infection. The expression of p16 was not a key indicator of the presence of HPV in all samples and a greater number of cases it is necessary to evaluate its applicability as a prognostic marker in this population.
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Francisco, Ana Lucia Noronha. "System of fluorescence spectroscopy in the evaluation of surgical margins for squamous cell carcinoma of the oral cavity in moments in situ and ex vivo = Sistema de espectroscopia de fluorescência na avaliação de margens cirúrgicas de carcinoma de células escamonas da cavidade oral nos momentos in situ e ex vivo." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/289799.
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Orientadores: Luiz Paulo Kowalski, Cristina KurachiTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: O tratamento mais empregado para os carcinomas de células escamosas da boca é a ressecção cirúrgica, sendo ou não acompanhada de radio e/ou quimioterapia. O tratamento é simplificado, diminuindo número de recidivas e aumentando a sobrevida, quando as lesões encontram-se em estágios iniciais, conforme localização anatômica e diagnóstico de margens cirúrgicas livres. Frente a isto, o desenvolvimento e aperfeiçoamento de técnicas para um diagnóstico precoce, assim como de uma acurada definição das margens cirúrgicas livres e correta delimitação da extensão do câncer boca, com o intuito de melhorar a qualidade de vida e a taxa de sobrevida desses pacientes, tornam-se de grande importância. A espectroscopia de fluorescência é uma ferramenta diagnóstica não invasiva que pode auxiliar na detecção do câncer em tempo real, com o potencial de fornecer sensibilidade e especificidade semelhantes ao diagnóstico clínico de profissionais experientes. É uma técnica relativamente simples, rápida e acurada que consiste em avaliar a composição bioquímica e a estrutura do tecido pelo espectro de fluorescência emitido por ele, após aplicação de um feixe de luz. Quando há progressão de um estado normal para um estado alterado, isso é refletido nas características espectrais da fluorescência dos tecidos, podendo ser correlacionada com o exame histopatológico destes tecidos. O objetivo do presente estudo consistiu em discriminar, na mucosa bucal, tecido sadio de neoplásico, por meio de espectroscopia de fluorescência avaliando as margens ressecadas cirurgicamente. As avaliações ocorreram nos pacientes do Departamento de Cirurgia de Cabeça e Pescoço e Otorrinolaringologia do AC Camargo Câncer Center. O estudo obteve aprovação dos Comitês de Ética em Pesquisa das instituições participantes. A amostra consistiu de 75 indivíduos nos quais se realizou a espectroscopia de fluorescência dos quais 45 pacientes eram portadores de carcinoma oral e 30 voluntários com mucosa oral clinicamente normal. 29 casos (64.4%) do sexo masculino e a média de idade foi de 61.3 anos. Foram realizadas biópsias e os resultados destas duas metodologias foram comparados, usando o diagnóstico histopatológico como padrão ouro, para identificar características espectrais de entre tecidos clinicamente não alterados das margens cirúrgicas da mucosa de voluntários. Os espectros foram classificados e comparados com a histopatologia para determinação da eficiência na discriminação diagnóstica empregando-se a fluorescência. A análise inicial foi qualitativa e após consistiu de processamentos matemáticos dos espectros com excitação nos comprimentos de 532 e 406nm. Observou-se a variabilidade entre os indivíduos, entre os sítios anatômicos, entre regiões da mesma lesão e entre tecido clinicamente normal de voluntários e tecido das margens cirúrgicas em momentos in situ. Foram observadas também grandes diferenças entre espectros in situ e ex vivo, em concordância com resultados de outros estudos. A acurácia da técnica variou em função do tipo de análise empregada, mas pode-se constatar o seu potencial de uso como instrumento auxiliar para avaliar margens cirúrgicas no câncer de boca
Abstract: The most widely used treatment for squamous cell carcinoma of the mouth is surgical resection, whether or not accompanied by radiation and/or chemotherapy. The treatment is simplified, reducing the number of recurrences and increasing survival when the lesions are in the early stages, according to anatomical site and diagnosis of disease-free surgical margins. The development and improvement of techniques for early diagnosis, as well as an accurate definition of disease-free surgical margins and correct delineation of the extent of the mouth cancer, is an important part of improving the quality of life and survival rate for these patients. Fluorescence spectroscopy is a noninvasive diagnostic tool that can aid in real-time cancer detection, with the potential to provide similar sensitivity and specificity to that of the clinical diagnoses of experienced professionals. It is a relatively simple, fast and accurate technique that assesses the biochemical composition and structure of the tissue by the fluorescence spectrum emitted after the application of a beam of light. When there is progression from a normal state to an altered state, this is reflected in the spectral characteristics of the fluorescence of the tissues, which may be correlated with the histopathological examination of these tissues. The aim of this study was to discriminate, in oral mucosa, healthy tissue through fluorescence spectroscopy by evaluating surgically resected margins. Assessments occurred in patients of the Head and Neck Surgery and Otorhinolaryngology Department of A.C. Camargo Cancer Center. The study was approved by the Research Ethics Committee of the institution. The sample consisted of 75 individuals who underwent fluorescence spectroscopy, of which there were 45 individuals with oral carcinoma and 30 healthy volunteers with normal oral mucosa. Twenty-nine cases (64.4%) were male and the mean age was 61.3 years. Biopsies were performed and the results of these two methods were compared using histopathology as the gold standard to identify spectral characteristics from clinically unchanged tissues to surgical margins of the mucosa of volunteers. The spectra were classified and compared with histopathology for determining the efficiency of diagnostic discrimination of employing fluorescence. The initial analysis was qualitative and consisted of following mathematical processing of the spectra with excitation in lengths of 532 and 406 nm. Variability was observed among individuals, anatomical sites, regions of the same lesion and clinically normal tissue of volunteers and the tissue of surgical margins in situ. Also observed were large differences between spectra in situ and ex vivo, in agreement with results of other studies. The accuracy of the technique varied according to the type of analysis used, but its potential use is noted as an aid to evaluated surgical margins in oral cancer
Doutorado
Estomatologia
Doutora em Estomatopatologia
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Abrahim, Naíza Menezes Medeiros, and 92-99962-0222. "Contribuição da Citopatologia Esfoliativa no diagnóstico de lesões de cavidade oral." Universidade Federal do Amazonas, 2017. https://tede.ufam.edu.br/handle/tede/6331.
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JUSTIFICATION: Oral cancer is considered worldwide as a major public health problem, more common in developing countries. The evolution can be insidious, and often diagnosed only in advanced stages with mutilating surgeries, associated or not with adjuvant treatment. As in other cancers, early diagnosis is a priority activity to perform less aggressive treatments and improving survival. An activity that can contribute to the early diagnosis of lesions with or without evident clinical repercussion is exfoliative cytology; technique is not yet implemented for the diagnosis of oral lesions in the Amazonas´s state. OBJECTIVES: The aim of this study is to evaluate the efficacy of the use of exfoliative cytology for the diagnosis of oral cavity and oropharyngeal lesions, prior to its surgical removal. MATERIAL AND METHODS: Patients with an incisional or excisional biopsy of oral cavity lesions attended at the Dental North Specialty Center and the Oncology Control Center Foundation of the State of Amazonas, both in Manaus-AM, were selected. Before completing the biopsy, and after patient's consent, a form was filled out to obtain clinical data, followed by photodocumentation of the lesion and collection of cytological material for the preparation of conventional smears and in a liquid medium. These were fixed and then stained by the Papanicolaou technique. The results of the cytology techniques were compared with each other and with those obtained in the histopathological evaluation. RESULTS: The study sample was composed of 50 patients, 30 females and 20 males. In 70% of the cases, the lesions were histological interpreted as benign and 30% as malignant. Squamous cell carcinoma was the most prevalent lesions, with 15 cases, the benign lesions preferential site was lip and the malignant lesions were tongue, the profile´s patients diagnosed with malignancy was of male patients, smoker and alcohol use. When comparing the two methods of cytology we obtained 100% sensitivity, specificity 97%, accuracy 97%, Kappa 0.91. Regarding the accuracy of the cytological and histopathological diagnosis, sensitivity was 86.6%, specificity 100%, PPV 100%, NPV 94.5%, Kappa 0.958 and accuracy 96%. CONCLUSIONS Traditional and liquid based cytology methods were able to identify and classify cell changes with characteristics of malignancy and have high sensitivity and specificity, without significant differences between the two techniques tested. Cytology techniques have proven reproducible and, if well indicated, can be routinely used for the early detection of malignant lesions.
JUSTIFICATIVA: O câncer de boca é considerado em todo o mundo como um grave problema de saúde pública, sendo mais incidente em países em desenvolvimento. Sua evolução pode ser insidiosa, sendo na maioria das vezes diagnosticado apenas em estágios avançados, em que cirurgias mutiladoras são realizadas, associadas ou não a tratamento adjuvante. Como em outras neoplasias, a precocidade do diagnóstico é atividade prioritária para a realização de tratamentos menos agressivos e na melhoria da sobrevida. Uma atividade que pode contribuir para o diagnóstico precoce de lesões orais e de orofaringe, com ou sem repercussão clínica mais evidente, é a citologia esfoliativa, técnica ainda não implementada para avaliação das referidas lesões no Estado do Amazonas. OBJETIVOS: Avaliar a eficácia do uso da citopatologia esfoliativa para diagnóstico de lesões de cavidade oral antes de sua remoção cirúrgica. MÉTODOS: Foram selecionados pacientes com indicação de biópsia incisional ou excisional de lesões de cavidade oral e orofaringe, atendidos no Centro de Especialidade Odontológica Norte e na Fundação Centro de Controle de Oncologia do Estado do Amazonas, ambos em Manaus-AM. Antes da realização da biópsia, e após anuência do paciente, foi realizado preenchimento de formulário para obtenção de dados clínicos, seguido de fotodocumentação da lesão e de coleta de material citológico para confecção de esfregaços convencional e em meio líquido. Estes foram fixados e então corados pela técnica de Papanicolau. Os resultados das técnicas de citologia foram comparados entre si e com aqueles obtidos na avaliação histopatológica. RESULTADOS: A amostra foi constituída de 50 pacientes, na qual 30 eram do sexo feminino e 20 do sexo masculino. E em 70% dos casos a lesão foi interpretada à histopatologia como de natureza benigna e em 30%, como maligna. A localização preferencial das lesões benignas foi em lábio e das lesões malignas, em língua, sendo que destas, a mais prevalente foi o carcinoma escamocelular, com 15 casos. O perfil dos pacientes com diagnóstico de malignidade foi de pacientes do sexo masculino, tabagistas e etilistas. Quando os dois métodos de citologia foram comparados entre si foram obtidos os seguintes resultados: sensibilidade 100%, especificidade 97%, acurácia 97%, Kappa 0,91. Com relação à acuidade do diagnóstico citológico com o histopatológico, a sensibilidade foi de 86,6%, a especificidade de 100%, o VPP 100%, o VPN 94,5%, Kappa 0,958 e acurácia 96%. CONCLUSÕES: Os métodos de citologia tradicional e em meio líquido foi capaz de identificar e classificar as alterações celulares características de malignidade e possuem alta sensibilidade e especificidade, sem que houvesse diferenças significativas entre as duas técnicas testadas. As técnicas de citologia se mostraram reprodutíveis e se bem indicadas podem ser utilizadas rotineiramente para detecção precoce de lesões malignas.
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Leimert, Mario, Tareq A. Juratli, Claudia Lindner, Kathrin D. Geiger, Johannes Gerber, Gabriele Schackert, and Matthias Kirsch. "An Extremely Rare, Remote Intracerebral Metastasis of Oral Cavity Cancer: A Case Report." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-132102.
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Distant brain metastases from oral squamous cell carcinomas (OSCC) are extremely rare. Here we describe a case of a 53-year-old man with a primary OSCC who referred to the neurosurgical department because of epileptic seizures. MR imaging revealed an enhancing lesion in the right parietal lobe. A craniotomy with tumor removing was performed. Histopathological examination verified an invasive, minimally differentiated metastasis of the primary OSCC. The patient refused whole brain radiation therapy and died from pulmonary metastatic disease 10 months after the neurosurgical intervention without any cerebral recurrence. To the authors’ knowledge, only two similar cases have been previously reported.
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Leimert, Mario, Tareq A. Juratli, Claudia Lindner, Kathrin D. Geiger, Johannes Gerber, Gabriele Schackert, and Matthias Kirsch. "An Extremely Rare, Remote Intracerebral Metastasis of Oral Cavity Cancer: A Case Report." Hindawi, 2013. https://tud.qucosa.de/id/qucosa%3A26371.
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Distant brain metastases from oral squamous cell carcinomas (OSCC) are extremely rare. Here we describe a case of a 53-year-old man with a primary OSCC who referred to the neurosurgical department because of epileptic seizures. MR imaging revealed an enhancing lesion in the right parietal lobe. A craniotomy with tumor removing was performed. Histopathological examination verified an invasive, minimally differentiated metastasis of the primary OSCC. The patient refused whole brain radiation therapy and died from pulmonary metastatic disease 10 months after the neurosurgical intervention without any cerebral recurrence. To the authors’ knowledge, only two similar cases have been previously reported.
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Foy, Jean-Philippe. "Caractérisation transcriptomique de l’hétérogénéité des lésions à potentiel malin et des carcinomes épidermoïdes HPV-négatifs de la cavité orale." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1086.
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La morbi-mortalité élevée des carcinomes épidermoïdes de la cavité orale (CECO), qui peuvent se développer à partir de lésions orales à potentiel malin (LOPM), rend indispensable le développement de nouvelles stratégies thérapeutiques. Le décryptage de l’hétérogénéité moléculaire aux différentes étapes de la carcinogénèse orale pourrait permettre de personnaliser les stratégies thérapeutiques de prévention et de traitement de ces cancers. Notre objectif était de caractériser l’hétérogénéité transcriptomique des LOPM et des CECO.Nous avons d’abord défini des signatures transcriptomiques associées aux changements histologiques de la carcinogénèse orale observés dans le modèle murin induit par le 4-NQO, montrant la pertinence de l’analyse de la dynamique temporelle du transcriptome pour améliorer la prévention des CECO. Cependant, ce modèle ne représentant qu’un sous-groupe particulier des CECO, nous avons ensuite étudié l’hétérogénéité inter-lésionnelle des LOPM en identifiant deux sous-types transcriptomiques principaux nommés « classical » et « immunological », qui sont caractérisés par différents biomarqueurs de risque de CECO.Au stade invasif, nous avons également étudié l’hétérogénéité transcriptomique des CECO HPV-négatifs entre les patients non-fumeurs non-buveurs (NFNB) et les patients fumeurs buveurs (FB). Le microenvironnement immunitaire était la principale différence biologique entre NFNB et FB, suggérant un bénéfice accru des immunothérapies chez les NFNB. Le profil transcriptomique de réponse antivirale observé dans les CECO des NFNB pourrait être en faveur de leur origine virale. En conclusion, l’hétérogénéité transcriptomique des LOPM et CECO suggère de personnaliser les stratégies thérapeutiques des patients porteurs de ces lésionsOral squamous cell carcinomas (OSCC), which may develop from oral premalignant lesions (OPL), are associated with a substantial morbidity and mortality. A better understanding of the molecular heterogeneity at different steps of oral carcinogenesis may help to refine prevention and treatment strategies of patients suffering from OPL and OSCC. Our goal was to decipher transcriptomic hetereogeneity of OPL as well as OSCC. Using the 4-NQO murine model of oral carcinogenesis, we first identified transcriptomic signatures that characterized the dynamics of gene expression changes through different stages of disease progression, and that could be relevant for refining prevention strategies. Because this model represents only a subgroup of patients suffering from OSCC, we then investigated inter-OPL molecular heterogeneity. We identified two distinct gene expression subtypes, which were named classical and immunological and were characterized by different biomarkers of cancer risk. At invasive steps, we investigated transcriptomic heterogeneity between HPV-negative OSCC from never-smoker never-drinker (NSND) and smoker drinker (SD) patients. The immune microenvironment was the main biological difference between OSCC from NSND and SD, suggesting higher clinical benefit of immunotherapies in OSCC from NSND. The antiviral gene expression profile of OSCC from NSND could suggest a viral origin.In conclusion, we investigated transcriptomic heterogeneity of OPL as well as OSCC, that could help to refine their prevention and treatment strategies
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Murphy, Justin Thomas. "Radioresistance in oral squamous cell carcinoma." Thesis, University of Hull, 2008. http://hydra.hull.ac.uk/resources/hull:770.
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Head and Neck Squamous Cell Carcinoma (HNSCC) is the sixth most common cancer accounting for approximately 6% of all cancers worldwide. However the distribution across the globe varies considerably. The majority of small tumours of the oral cavity and upper aerodigestive tract, in the absence of metastatic disease, can be successfully treated with surgery or radiotherapy. Despite this most small tumours of the oral cavity are now treated with surgery as the primary treatment modality with radiotherapy being reserved for adjuvant therapy, palliation or in patients unfit for surgery. Radiotherapy is also used in cases where there is doubt about the completeness of resection and where adverse histological characteristics are present. Unfortunately, on average about 10% of tumours treated in this way are resistant to radiotherapy, developing tumour recurrence within the original radiotherapy field during the ensuing 12 months. Patients with radioresistant tumours are not only receiving a therapy that is unnecessary but are also being put at risk of potentially serious complications, e.g. osteoradionecrosis of the cervical spine. The primary aim of this thesis was to investigate the mechanism of radioresistance and create an in vitro model of a radioresistant oral squamous cell carcinoma. The methods of cell culture, microarray analysis and immunohistochemistry were employed to this end. Two novel radioresistant cell lines, PE-CAPJ41RR and PE-CAPJ49RR, were created and a number of targets identified using microarray analysis. Immunohistochemistry was used to investigate the relationship EGFR, Bcl-2, BAX and COX-2 had with radiotherapy response.
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Ash, Cecil Samuel. "Mandibular invasion in oral squamous cell carcinoma." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq23202.pdf.
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Sun, Li. "Molecular cytogenetics of oral squamous cell carcinoma." Click to view the E-thesis via HKUTO, 2002. http://sunzi.lib.hku.hk/HKUTO/record/B38627887.
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Lim, Kue Peng. "Fibroblasts in human oral squamous cell carcinoma." Thesis, University of Bristol, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.503859.
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The tumour microenvironment is known to play an important role in tumour development and progression. The diversity and role of stromal fibroblasts in human oral cancer, however, is unknown. In this study, fibroblasts were oral cancer, however, is unknown. In this study, fibroblasts were isolated from cultures of normal oral mucosa, oral epithelial dysplasia and mortal and immortal oral carcinomas, the latter malignancy being genetically unstable. Using global gene expression profiling, we demonstrated that fibroblasts clustered according to their tissue of origin.
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Sun, Li, and 孫莉. "Molecular cytogenetics of oral squamous cell carcinoma." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B36544267.
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Yap, Lee Fah. "Molecular characterization of oral squamous cell carcinoma." Thesis, University of Bristol, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435716.
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Kim, Hyung Jun. "Surgical management of oral squamous cell carcinoma infiltrating mandible." Diss., lmu, 2008. http://nbn-resolving.de/urn:nbn:de:bvb:19-98143.
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Sawair, F. A. "Prognostic indicators of outcome for oral squamous cell carcinoma." Thesis, Queen's University Belfast, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390863.
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Gemenetzidis, Emilios. "The role of FOXM1 in oral squamous cell carcinoma." Thesis, Queen Mary, University of London, 2010. http://qmro.qmul.ac.uk/xmlui/handle/123456789/492.
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FOXM1 transcription factor regulates the expression of a multitude of genes, which are important for cell proliferation, mitosis, and differentiation. Although it is abundantly expressed in majority of human solid tumours, its role in early stages of human neoplasia remains unclear. Oral squamous cell carcinoma (OSCC) is characterized by sequential genomic alterations, which lead to invasive malignancy. In this study, it is shown that FOXM1 is significantly upregulated in early oral pre-malignant and OSCC tissues and cultured keratinocytes. Furthermore, the current study suggests that FOXM1B is the main isoform driving the cell cycle dependent expression of FOXM1, and that it is expressed mainly at the G2 phase of human epithelial keratinocytes. In an attempt to understand why FOXM1 precedes epithelial malignancy, the present study investigated 1) the genomic profile of FOXM1B overexpressing human epithelial keratinocytes, and 2) whether FOXM1B overexpression interferes with the innate program of keratinocyte differentiation, which is frequently reported as being the earliest oncogenic event in epithelial neoplasia. First, by using a high-resolution Affymetrix single nucleotide polymorphism (SNP) mapping technique, this study provides the first evidence that FOXM1B overexpression alone in primay human keratinocytes was sufficient to induce genomic instability, mainly in the form of copy number alterations. FOXM1B overexpression also cooperated with damaging agents relevant to human epidermal (UVB) and oral epithelial cancer (Nicotine), to promote genomic instability in human keratinocytes. Second, by using a 3D-organotypic culture model of oral mucosa, sustained overexpression of FOXM1 was found to induce a hyper-proliferative phenotype with suprabasal proliferation, exhibiting perturbed markers of epithelial differentiation such as cytokeratin 13 and filaggrin, resembling early oral dysplastic epithelium. Based on these observations it is hypothesised that aberrant upregulation of FOXM1B serves as a ‘first hit’ whereby cells acquire genomic instability, and an abnormal differentiation program. The latter event promotes epithelial proliferation at the expense of terminal differentiation, allowing sufficient time for the accumulation of additional genetic aberrations/mutations required for tumour promotion and expansion. The Role of FOXM1 in Oral Squamous Cell Carcinoma
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Qadir, Fatima. "Cellular and molecular signature of oral squamous cell carcinoma." Thesis, Queen Mary, University of London, 2018. http://qmro.qmul.ac.uk/xmlui/handle/123456789/39763.
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Oral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide. It is a result of numerous aetiological factors such as genetic predisposition, smoking, excessive alcohol consumption and viruses such as the human papilloma virus. Due to late diagnosis it has a high mortality and morbidity rates which has remained unchanged over the last 5 decades. Currently no screening is available for high risk patients for better monitoring. Diagnosing OSCC relies on histopathology of biopsy tissue, reviewed for dysplasia and advancing lesions. Although the technique has been used for decades for successful diagnosis it fails to identify the molecular signature of OSCC which appears much before the visual signs. It also falls short in predicting the malignant transformation of pre-malignant oral lesions. Identifying the molecular and genetic changes leading to OSCC lesion will aid in more specific (quantitative) and early diagnosis of the disease reducing the financial burden of treating late-stage OSCC patients on the healthcare system. This study focuses on developing new adjuncts which can be used alongside histopathology for early diagnosis. There is a need to monitor high risk patients through non-invasive methods causing less patient discomfort. We therefore explored the potentials of exosomes which are extracellular vesicles secreted by normal and tumour cells. They can be isolated from body fluids such as blood and saliva. In cancer biology exosomes offer both diagnostic and therapeutic advantage. Their involvement in cell-cell communication indicates their influence in tumour development, progression, metastasis and therapeutic efficacy. Exosomes released by cancerous cells carry numerous biomarkers, which are passed on to healthy cells via microenvironment, causing stromal and angiogenic activation along with immune escape. In this study exosomes were successfully isolated from body fluids (blood, saliva and plasma) and cell line supernatant through ultracentrifugation and characterised by visual and particle size quantification techniques including Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), Zetasizer and Nanosight Tracking Analysis (NTA). Exosomal specific membrane proteins were identified through Western blotting. 5 We report the presence of a potential protein biomarker located exclusively on the outer membrane of cancer exosomes. Since body fluids consist of a heterogeneous population of exosomes derived from multiple cell types, such surface biomarker can potentially be used to isolate OSCC exosomes. Characterisation of exosomal mRNA cargo was done using Agilent Bioanalyzer (for RNA quantity and quality assurance) and reverse transcription-quantitative PCR (RT-qPCR; for gene specific quantitation). Functional significance of exosomes was studied by transfecting normal oral keratinocyte cells with self and cancer-derived exosomes. Through gene-expression microarray and subsequent RT-qPCR verification, we report a panel of differentially expressed genes involved in essential cellular functions being modulated by exosome transfection. A previously developed molecular diagnostic system by our research group called quantitative malignancy index diagnostic system (qMIDS) based on FOXM1 oncogene and its downstream targets was validated on archival formalin fixed paraffin embedded OSCC patient biopsy samples. We report that qMIDS index successfully correlates with the disease stages including dysplasia, tumour and lymph node metastasis. Furthermore, through meta-analysis of 8 OSCC microarray studies we identified a panel of six genes including PLAU, FN1, CDCA5, CRNN, CLEC3B and DUOX1 (q6) which are able to identify two clinically distinct sub-groups of OSCC patient population. Through RT-qPCR the expression of q6 biomarkers was established in 100 OSCC biopsy samples. This information can be of immense importance in developing personalized treatment strategies based on the molecular makeup of the presenting tumour.
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supsavhad, wachiraphan. "Novel Molecular Targets for Feline Oral Squamous Cell Carcinoma." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1471628009.
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Davidson, Matthew Alexander. "Analysis of potential driver genes in oral squamous cell carcinoma." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/9018/.
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The 5-year survival rate of head and neck squamous cell carcinoma (HNSCC) has remained at ~50% for over 50 years. HNSCC is categorised by multiple anatomical sites, but oral (oral SCC) and oropharyngeal squamous cell carcinoma (OPSCC) account for approximately 90% of all cases. At the time of writing, only one targeted agent, cetuximab (a monoclonal antibody targeting the epithelial growth factor receptor), has been approved for the treatment of recurrent/metastatic HNSCC. However, despite the high expression of EGFR in oral SCC tumour samples, the clinical benefit of cetuximab has been modest thus far. Using a phenotypic screening approach, I sought to identify putative therapeutic targets. A whole genome siRNA screen carried out using an aggressive patient-derived cell line (‘Liv7k’) in normoxic and hypoxic conditions provided the foundation for this project. In addition, a drug-repurposing screen tested the efficacy of 1,351 compounds, approved for cancer and non-cancer indications. A number of approaches were used to identify potential targets, including a whole genome siRNA screen in normoxic and hypoxic conditions, a drug-repurposing screen, and a data multiplexing approach combining the two screens with pathway analysis and datasets from The Cancer Genome Atlas and the International Cancer Genome Consortium. Genomic characterisation of oral cancer cell lines confirmed the importance of a previously identified frequently amplified region of chromosome three, which contains a number of driver genes in HNSCC. In addition, a differential susceptibility of oral SCC cells in hypoxia formed the basis of a line of inquiry centred on triglyceride and ether lipid metabolism. Finally, compound screening identified a dependence of oral SCCs on cysteinyl leukotriene signalling, which is involved in inflammatory conditions such as asthma.
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Emich, Helena. "Clinical implications of cancer stem cell properties in oral squamous cell carcinoma." Thesis, Queen Mary, University of London, 2014. http://qmro.qmul.ac.uk/xmlui/handle/123456789/8479.
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CD44 has been described as a marker of cancer stem cells in oral squamous cell carcinoma (OSCC). The main objective of this study was to characterise expression of CD44 in both fresh samples of human OSCC and in cell lines generated from them, and to examine its correlation with selected clinicopathological parameters of the tumours of origin. The epithelial fraction in 20 fresh OSCC samples was identified by the standard method using the negative selection technique with antibodies against non-tumour cells. A novel method of identifying the epithelial fraction, termed positive selection, was also developed and used for analysis of 14 additional OSCC samples. This new method, using epithelial-specific antibodies, led to a considerable improvement in the efficiency and the accuracy of the procedure. The frequency of CD44+ cells in the epithelial fraction of the tumour specimens was assessed by FACS and varied widely (3-97%). High frequency of CD44+ cells in tumour samples was found to be associated with high tumour grade, discohesive invasion front and presence of lymph node metastases (p<0.01, as calculated with Spearman’s ranked test and Fisher’s exact test). It was also observed, that the percentage of CD44+ cells changes when cells isolated from tumour samples are propagated in culture. Nearly all cells in cell lines generated from OSCC samples showed CD44 expression when analysed by FACS. However, a markedly higher level of CD44 expression (as assessed by median fluorescence intensity for cell surface CD44) was found for early passage cell lines generated from metastatic OSCC and lymph node metastases as compared to cell lines generated from nonmetastatic OSCC. These findings show that a high frequency of CD44+ cells in fresh OSCC tissue and a high level of CD44 expression in cultured OSCC cells correlate 11 with more aggressive tumour behaviour. These results might provide important information of prognostic and therapeutic value.
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Escriu, Carlos. "The role of Mst2 in oral squamous cell cancer progression." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708173.
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Sartor, Marina. "Analysis of G1 checkpoint components in Oral Squamous Cell Carcinoma (OSCC)." Thesis, King's College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325318.
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Rawal, Yeshwant B. "Pro and Antioxidant Modulation of the Oral Squamous Cell Carcinoma Phenotype." The Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=osu1419873714.
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Han, Byungdo B. "Chemoprevention of Oral Squamous Cell Carcinoma: Extending Therapeutic Parameters of Fenretinide." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429726539.
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Wilcock, Paul. "A systems biology approach for investigating oral squamous cell carcinoma (OSCC)." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/a-systems-biology-approach-for-investigating-oral-squamous-cell-carcinoma-oscc(8ec3728b-1928-450f-b467-76996fa970fb).html.
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A systems biology approach was adopted in order to assess various aspects of the disease oral squamous cell carcinoma. Three main aims were addressed; assess the ability of CoCl2 to mimic the hypoxic response in a eukaryotic cell line, assess the role of PDE4D in oral squamous cell carcinoma (OSCC) and the construction of a normoxic/hypoxic mathematical model to identify therapeutic targets.Cancer cells often acquire a revised metabolism which aids in initiation, survival and progression of the tumour. This is predominantly due to the transcription factor HIF-1 which is activated under hypoxic conditions. Certain compounds such as cobalt chloride (CoCl2) have been used extensively to inhibit the degradation of HIF-1α and simulate hypoxia. CoCl2 is likely to have off-target effects on metabolism; these effects were examined when exposing human telomerase reverse transcriptase (hTERT) cells to 100μM CoCl2. Gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) based metabolomics were utilised in combination with ELISA assays for HIF-1α and ATP. Central metabolism was accurately mimicked when hTERT cells were subjected to 100μM CoCl2, however; it was apparent that this concentration of CoCl2 does not induce an equal extent of hypoxia as 1% oxygen. A number of off-target effects of CoCl2 were observed in secondary metabolism, specifically in lipids and fatty acids. In conclusion, CoCl2 should be used with caution as a hypoxic mimicker with the caveat that interpretation of results should be restricted to its effects on central metabolism.The transcription factor CREB has the ability to regulate approximately 4000 genes, a number of which are associated with cancer initiation and progression. Cyclic adenosine monophosphate (cAMP) is required to activate CREB and is partially regulated through its degradation via the enzyme phosphodiesterase type 4D (PDE4D). A homozygous deletion of PDE4D has been associated with OSCC; however; the exact consequence of this deletion has not been fully elucidated. PDE4D was knocked down in the OSCC cell line BicR16 and cellular proliferation, migration, resistance to ionising radiation and central metabolism was investigated using MTT, scratch, clonogenic and GC-MS, respectively. The knockdown resulted in an increase in proliferation, migration and radiation resistance suggesting the role of PDE4D as a TSG. Amino acids, cholesterol, fatty acids, carbohydrates and TCA intermediates were found to be altered in concentration.A mathematical model of glycolysis, TCA and glutaminolysis under normoxia and hypoxia was constructed through the amalgamation of two established models from the literature. New reactions, parameters and metabolite concentrations were added and unnecessary entities were deleted. COmplex PAthway SImulator (COPASI) was utilised to construct the model before validating the model using experimental data from the literature and steady state and flux analyses. Sensitivity analysis and a reduction in external glucose and glutamine were mimicked and the alterations in hypoxic and normoxic metabolism analysed. The reactions vCSII, vGS, vPGK and vGII were identified as potential therapeutic targets which may affect metabolism in hypoxia only. However, certain validation methods proved unsuccessful and hence the model requires further work before attempting the analyses again.
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Towle, Rebecca. "The molecular characterization of the progression of oral squamous cell carcinoma." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/59026.
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Oral squamous cell carcinoma (OSCC) is the most common subtype of head and neck cancer and has a relatively low five year survival rate of ~50%. One of the reasons for this high mortality rate is that patients are generally diagnosed at late stages. OSCC develops through a typical histological progression and although lesions in the oral cavity are visible at the premalignant stage, it is not possible to predict which lesions will progress based on histology alone. In-depth analysis of genome-wide molecular alterations may identify novel genes or pathways that can be used as biomarkers or therapeutic targets in order to improve survival rates of this disease.
In this thesis, I perform DNA methylation, gene expression and miRNA profiling on a panel of patient tissue samples, each with a paired adjacent normal, dysplasia and either a carcinoma in situ or squamous cell carcinoma, taken from a single contiguous disease field within a patient’s oral cavity. My hypotheses are that the epigenetic landscape of OSCC becomes progressively more deregulated throughout the different histological stages and that the most frequently altered molecular events identified at the dysplasia stage may be crucial for premalignant disease development and progression. A high level of deregulation in both methylation and miRNA patterns as the disease progresses is observed, and a number of highly frequent molecular events are identified. Several of these molecular events are then functionally validated to assess the ability to contribute to tumorigenesis in oral premalignant lesions.
Taken together, this thesis provides one of the most comprehensive epigenetic analyses of paired normal, dysplasia and CIS/SCC biopsies with regards to DNA methylation and miRNA profiling. In addition to providing a deeper insight into the molecular mechanisms at play within the premalignant lesions, we also validate the ability of these mechanisms to directly contribute to tumorigenesis.Medicine, Faculty of
Graduate
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Yoshikawa, Hiroto. "Feline oral squamous cell carcinoma| A comprehensive approach to improve treatment outcome." Thesis, Colorado State University, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3593468.
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Feline oral squamous cell carcinoma (SCC) is a devastating disease that responds poorly to traditional treatment modalities. The tumor location directly impacts the patient's ability to eat and drink, and immediate intervention to alleviate clinical signs is important. To design better treatment strategies it is paramount to understand the underlying biological behavior of this poorly defined tumor. This research takes a comprehensive approach in attempt to understand this disease. A number of assays have been developed and applied to elucidate underlying biology. New imaging modalities have been used to better stage the disease and define tumor location. Finally, patients were treated with a new radiation therapy modality, stereotactic radiation therapy (SRT), and outcome was correlated with the biological assays for potential predictive value.
The goal of the prospective study described in Chapter 2 was to compare gross tumor volume measurements using 18F-FDG PET vs. those using computed tomography (CT) for SRT planning in cats with oral SCC. Twelve cats with confirmed oral SCC underwent pretreatment 18F-FDG PET/CT. Gross tumor volumes based on contrast-enhanced CT and 18F-FDG PET were measured and compared between cats. Mean PET gross tumor volume was significantly smaller than mean CT gross tumor volume in the mandibular/maxillary SCC group (n=8, P=0.002) and for all cats (n=12, P=0.006), but not for cats with lingual/laryngeal SCC (n=4, P=0.57). Mismatch fraction analysis revealed that most of the lingual/laryngeal patients had a large region of high-18F-FDG activity outside of the CT gross tumor volume. This mismatch fraction was significantly greater in the lingual/laryngeal group than the mandibular/maxillary group ( P=0.028). The effect of poor spatial resolution of PET imaging was greater when the absolute tumor volume was small. Findings from this study indicated that 18F-FDG PET warrants further investigation as a supplemental imaging modality in cats with oral SCC because it detected presumed regions of primary tumor that were not detected on CT images.
For canine and feline patients with tumors in the head region, simultaneous irradiation of the primary tumor and mandibular and retropharyngeal lymph nodes (LNs) is often indicated. The purpose of this study described in Chapter 3 was to assess the reliability of a planning target volume (PTV) expansion protocol for secondary targets (LNs).
Information about the molecular biology of feline oral SCC is still limited. In Chapter 4, 22 archived tumor samples of feline oral SCC were evaluated to develop immunohistochemical assays and to determine if there was correlation to clinical parameters. Immunohistochemistry for Ki67, MVD, and EGFR was performed and scored. Patient survival information was obtained from the medical records. These molecular markers as well as MI were correlated with tumor locations and patient survival time. The 22 tumors showed wide variation in Ki67, MI, MVD, and EGFR. Tongue SCC expressed higher MVD than mandibular/maxillary SCC (P=0.088).
Cancer stem cell or tumor initiating cell (TIC) theory and telomere biology are actively studied fields in human head and neck (H&N) cancer. In feline oral SCC, which has been advocated as a feline model for human H&N cancer, our knowledge about the TIC and telomere/telomerase biology is limited. Protein expression levels of putative TIC markers of human H&N cancer, CD44 and Bmi-1, were immunohistochemically evaluated for their possible role as prognostic markers in 20 patients with feline oral SCC who underwent SRT. This patient population was part of a clinical trial and information relevant to PFI and ST was available. A combined technique of fluorescent in-situ hybridization and immunofluorescent staining was used to determine telomere length ratio (fractions of very short telomere/average length telomere in the putative cancer stem cells) in the putative TICs that were positive for CD44 and Bmi-1. This was also correlated with treatment outcome. (Abstract shortened by UMI.)
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Windrich, Martin. "Automatic quantification of speech intelligibility of adults with oral squamous cell carcinoma /." Erlangen, 2008. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000254063.
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Bergkvist, Gurå Therese. "Role of epidermal growth factor receptor in feline oral squamous cell carcinoma." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/5542.
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Feline oral squamous cell carcinomas (FOSCCs) are locally aggressive tumours and a common cause of mortality and morbidity. Current treatment options are rarely successful and animals are frequently euthanised upon diagnosis due to their grave prognosis. Epidermal Growth Factor Receptor (EGFR) is a tyrosine kinase receptor which is frequently dysregulated in SCC of the head and neck (HNSCC) in man. Recent advances in human medicine have identified EGFR as a therapeutic target in HNSCC. In this study the role of EGFR in FOSCC was investigated. Sixty seven biopsy samples were immunohistochemically labelled for EGFR and Ki67, a proliferation marker. The tyrosine kinase region of feline EGFR was cloned and sequenced, and six small interfering RNAs (siRNAs) targeting the tyrosine kinase region were developed. The most effective siRNA as well as an EGFR specific tyrosine kinase inhibitor, gefitinib, was then used on a feline SCC cell line (SCCF1), and the effect of EGFR targeting alone, or in combination with irradiation, on the cell line was determined. The majority of the biopsy samples were labelled positively for EGFR and Ki67, and high proliferation corresponded with poor prognosis. The siRNA caused reduction in EGFR mRNA by Real-Time Polymerase Chain Reaction and protein levels as assessed by western blot analysis. Reduced cell proliferation and migration were also observed by proliferation assays and scratch assays respectively. Combining EGFR knockdown with irradiation caused an additive effect on the ability of the cell line to form colonies. These results support the role of EGFR as a potential therapeutic target in FOSCCs.
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Chu, Hao Wei, and 朱浩維. "Identification of Salivary Biomarkers for Detecting Oral Cavity Squamous Cell Carcinoma by Quantitative Proteomics." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/53496838952583369676.
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Chu, Hao Wei, and 朱浩維. "Identification of Salivary Biomarkers for Detecting Oral Cavity Squamous Cell Carcinoma by Quantitative Proteomics." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107CGU05114056%22.&searchmode=basic.
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Huang, Mei-Tzu, and 黃美慈. "The Effects of Treatment Modality on Survival in Patients with Oral Cavity Squamous Cell Carcinoma." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/30927238897361536996.
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碩士國立陽明大學
衛生福利研究所
98
Background: Oral cavity Squamous Cell Carcinoma was the important issue in Taiwan. The prevalence and mortality increase rapidly. Several studies have discussed the prognosis of the patients under different treatments in western countries. However, there are few studies concerning the territory in Taiwan. This study is to explore the treatment outcome of oral cavity Squamous Cell Carcinoma by using the secondary databases of government. Objective: To investigate the effect of first treatment modality on survival in patients with oral cavity squamous cell carcinoma. Methods: The study was retrospective cohort study. Patients who diagnosed with oral cavity squamous cell carcinoma in 2004-2005 were collected from Taiwan Cancer Data Base (TCDB) and Cancer Registry System (CRS), and using encrypted identification number for linking the related database. Stage was used as a stratification variable in the analysis. Using stepwise Cox' s regression analysis, to examined the effect of first treatment modality in four months after first diagnosis on survival in patients with oral cavity squamous cell carcinoma. Results: There were 7,064 patients included. The treatment rate in four months was 88.82％; the three common treatment modalities were surgery(39.32%)、postoperative radiotherapy(17.90%)、postoperative radiotherapy then chemotherapy(8.05%). surgery alone had the best prognosis in each stage. The stageⅠ postoperative radiotherapy and postoperative radiotherapy then chemotherapy had a significantly higher adjusted hazard ratio than surgery alone(1.89 and 1.86 respectively). The stageⅡ was 1.80 and 2.37 respectively; the stageⅢ was 1.66 and 1.37 respectively; the stageⅣA was 1.09 and 1.00 respectively. Conclusions: Treatment modality is a crucial attribute to prognosis in patients with oral cavity squamous cell carcinoma. Careful considering the patients’ characteristics and using the most adequate treatment are importment.
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Liu, Chiao Rou, and 劉巧柔. "Identification of Therapeutic Targets and Biological Pathways for Oral Cavity Squamous Cell Carcinoma using Proteomics Approaches." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107CGU05604003%22.&searchmode=basic.
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Cho, Kai Lun, and 卓楷倫. "The role of ATM and H2AX protein expression in oral cavity squamous cell carcinomas." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/97015659783958994557.
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碩士長庚大學
生物醫學研究所
104
DNA double-strand break is one of the types of DNA damage, which developed two cell repair mechanisms: homologous recombination and non-homologous end join. Some important tumor suppressor genes are involved in these repairs, such as ATM, Chk2, BRCA1, and BRCA2. In early stage of DNA damage, ATM and other protein kinases phosphorylate H2AX at Ser139 to form γH2AX. ATM and γH2AX overexpress at damage site until the completion of DNA repair. Recent studies indicated that cigarettes, alcohol and betel quid are correlated to oral cancer in Taiwan and smoking leads to DNA damage and DNA mutation which could subsequently play important roles in the process of carcinogenesis. Our purpose is to investigate the role of DNA repair proteins, ATM, pATM and H2AX, in oral cavity squamous cell carcinomas (OSCC). The method to detect protein expression was immunohistochemistry (IHC) staining. Our results showed that ATM was correlated with tumor depth (p = 0.000), tumor stage (p = 0.013) and lymphatic vessel invasion (p = 0.027); pATM was correlated with tumor depth (p = 0.027), tumor size(p = 0.001), tumor stage(p = 0.029), cigarette smoking(p = 0.033), skin invasion (p = 0.014) and bone invasion (p = 0.000); H2AX was correlated with cancer site (p = 0.011), cigarette smoking (p = 0.008) and betel quid chewing (p = 0.012). We also found that ATM was significantly correlated with overall survival (p = 0.023) and also correlated with disease-free survival (p = 0.043) in patients who had adjuvant chemoradiation therapy. ATM, pATM and H2AX play important roles in oral cancer development. The overexpression of ATM could provide survival benefits in OSCC patients in Taiwan.
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Schwock, Joerg. "Inhibition of Hsp90 and its Client Kinase FAK has Therapeutic Potential in Squamous Cell Carcinomas of the Uterine Cervix and Oral Cavity." Thesis, 2010. http://hdl.handle.net/1807/26517.
Full textAbstract:
Heat shock protein 90 (Hsp90) is an essential and conserved chaperone, required for the conformational maturation and stability of many signaling kinases. We hypothesized that the functional pleiotropism of Hsp90 can be exploited during pharmacological inhibition causing simultaneous restraint of tumor growth as well as suppression of distant spread. Recognizing the lack of therapeutic options in advanced and metastatic squamous cell carcinomas (SCC) of the uterine cervix as well as the oral cavity, this dual concept was tested in corresponding cell lines and xenografts, and correlated with clinical data on client protein expression. Examination of the cell cycle response to Hsp90 inhibition revealed a G2/M-arrest in a panel of four cervical cancer cell lines and a contribution of abnormal mitosis to apoptosis induction in vitro. Although limited to intraperitoneal application, in vivo evidence of biological activity including heat shock response and decreased client kinase phosphorylation was seen with the geldanamycin derivative 17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG). Importantly, focal adhesion kinase (FAK) signaling and associated functional parameters were inhibited by the drug treatment. Functional significance of FAK as a client was confirmed using a molecular model based on FAK-related non-kinase (FRNK) expression. Dependency on FAK appeared to be a requirement for full response to FRNK as well as 17-DMAG, and was observed in the mesenchymal-like cervical cell line SiHa. FAK expression and E-cadherin loss were features found in both cervical and oral malignancies, but absent from normal mucosa of either anatomic site. Particularly high FAK expression was noted in oral SCC with sarcomatoid features. Thus, we conclude that Hsp90 inhibition has potential in the treatment of advanced and metastatic SCC of cervical and oral origin. The further examination of novel Hsp90-targeting compounds as well as strategies focused on other components of the Hsp90 chaperone complex seems warranted.
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Ribeiro, Ilda Patrícia Tavares da Silva. "Head and Neck Squamous Cell Carcinoma: integrating genomic, epigenetic and transcriptomic data - from bench to clinical applications." Doctoral thesis, 2017. http://hdl.handle.net/10316/79613.
Full textAbstract:
Tese de doutoramento do Programa Interuniversitário de Doutoramento em Envelhecimento e Doenças Crónicas, apresentada à Faculdade de Medicina da Universidade de CoimbraHead and neck squamous cell carcinoma (HNSCC) is an emergent health problem worldwide. These tumors present heterogeneity at phenotypic, aetiological, biological and clinical level. In developed countries, smoking and alcohol are implicated in the increase of HNSCC cases, and human papillomavirus is an important risk factor, especially in the rise of oropharyngeal tumors without smoke and alcohol habits. A significant percentage of HNSCC patients develop loco-regional and distant recurrences. Even with progresses in surgery, radiation and chemotherapy, approximately half of all patients die of the disease. Risk stratification for HNSCC is essencial in order to decrease mortality and improve quality of life of the patients. The great HNSCC heterogeneity makes difficult to understand the molecular carcinogenesis process as well as to develop early detection and therapeutic strategies for these tumors. Nowadays, the majority of genome-wide molecular profiling studies of HNSCC are limited to single approaches, which hampers the identification of accurate and robust biomarkers of early diagnosis and prognosis. Indeed, there is a lack of proven biomarkers for predicting clinical outcomes and response to treatment. The present work aimed to perform a molecular characterization of HNSCC in order to predict recurrence/metastasis development and signaling pathways associated to targeted therapy and resistance to conventional drugs through the identification of different molecular groups with apparently different survival profiles using genomic, epigenetic and transcriptomic approaches. We analyzed the same HNSCC patients through different molecular technologies, being the identified biomarkers and molecular signatures validated with TCGA (The Cancer Genome Atlas) data. First, we performed a direct genetic and epigenetic characterization of HNSCC patients, using specific Multiplex Ligation-dependent Probe Amplification (MLPA) and Methylation-Specific MLPA (MS-MLPA) probe panels. We reported different genetic signatures related to tumor stage and anatomic site as well as tobacco use. Additionally, specific genomic and epigenetic signatures associated to patients' risk of recurrence/metastasis development after treatment of primary tumor and also to survival were identified. The genetic analysis of non-tumor samples (from surgical margins) revealed some imbalances similar to those identified in the tumor samples, which reinforce the importance of molecularly analyze the high-risk patients even before the visible morphological changes and also the suspicious lesions in order to early diagnose these tumors and their recurrences. Secondly, we moved forward to a high-throughput genomic and transcriptomic approaches and we identified molecular signatures with capability to predict the recurrent/metastatic disease development and clinical outcome. In these studies using either direct probe panels or genome-wide approaches we identified the most common chromosomal regions with imbalances and altered genes. As expected, whole-genome techniques revealed new chromosomal regions and genes that seem to have a role in HNSCC development and behavior. Overall, through these comprehensive genomic, epigenetic and transcriptomic characterization we identified biomarkers and molecular signatures of prognosis and survival, which open the door for personalized medicine in HNSCC patients. Finally, we applied these genomic and epigenetic technologies to perform a molecular characterization of four paradigmatic HNSCC patients in order to prove the benefit of these molecular knowledgement to the clinical management of the HNSCC patients. Several chromosomal regions and genes related to radiation and/or chemotherapy resistance and to patients' prognosis and survival were identified, which could help and guide the type or intensify of treatment modalities. Moreover, molecular characterization of commercial HNSCC cell lines and primary cell cultures established from these patients was conducted, which revealed the ploidy and the complex structural chromosomal rearrangements of HNSCC tumors. This comprehensive characterization enables cell models for further studies both in radiation and pharmacogenomics fields, as well as to understand the molecular mechanisms of HNSCC development and progression. With this work we performed a robust molecular characterization of HNSCC, using different omic approaches in the same tumor samples, which allowed the identification of new prognostic biomarkers and molecular signatures with potential to be translated to clinical practice.
O carcinoma epidermóide da cabeça e pescoço (CECP) é um problema emergente de saúde em todo o mundo. Estes tumores são heterogéneos a nível fenótipico, etiológico, biológico e clínico. Nos países desenvolvidos, o tabaco e o álcool estão implicados no aumento do número de casos de CECP e o papiloma vírus humano é um fator de risco importante para o aumento dos tumores da orofaringe não relacionados com hábitos tabágicos e de álcool. Uma percentagem significativa de doentes com CECP desenvolve recidivas loco-regionais e à distância. Mesmo com os progressos na cirurgia, radioterapia e quimioterapia, cerca de metade de todos os doentes morre devido ao CECP. A estratificação do risco de CECP é essencial de forma a contribuir para a diminuição da mortalidade e melhoria da qualidade de vida destes doentes. A heterogeneidade do CECP dificulta por um lado a compreensão dos processos moleculares da carcinogénese e por outro lado o desenvolvimento de estratégias de deteção precoce e de terapêutica. Atualmente, a maioria dos estudos moleculares de grande escala são restritos, o que dificulta a identificação robusta e precisa de biomarcadores de diagnóstico e prognóstico. De facto, há falta de biomarcadores para predizer o desenlace clínico e resposta ao tratamento. O presente trabalho teve como objetivo caraterizar molecularmente o CECP de forma a prever o desenvolvimento de recidivas/metástases e a identificação de vias de sinalização associadas a terapias alvo e resistência às terapias convencionais, através da identificação de diferentes grupos moleculares com diferentes sobrevivências, usando abordagens de genómica, epigenética e transcriptómica. Neste estudo, analisámos os mesmos doentes com CECP usando diferentes tecnologias moleculares, tendo validado os biomarcadores e assinaturas moleculares identificados usando dados do portal TCGA (The Cancer Genome Atlas). Em primeiro lugar, realizámos uma caraterização genética e epigenética do CECP direcionada, utilizando painéis de sondas específicos de Multiplex Ligation-dependent Probe Amplification (MLPA) e Methylation-Specific MLPA (MS-MLPA). Identificaram-se diferentes assinaturas genéticas relacionadas com o estadio do tumor e as localizações anatómicas, bem como com o consumo de tabaco. Adicionalmente, uma assinatura genética e epigenética associada ao risco dos doentes desenvolverem recidivas/metástases após o tratamento do tumor primário e também associada à sobrevivência, foi identificada. A análise genética das amostras não tumorais (provenientes das margens cirúrgicas) revelou alguns desequilíbrios similares aos identificados nas amostras tumorais, o que reforça a importância de analisar molecularmente os doentes de elevado risco mesmo antes de qualquer alteração morfológica visível e também das lesões suspeitas, de forma a diagnosticar precocemente estes tumores e as suas recidivas. Na segunda parte do estudo utilizámos abordagens genómicas e transcriptómicas de larga escala e, identificámos assinaturas moleculares capazes de prever o desenvolvimento de recidivas/metástases e evolução clínica dos doentes. Estes estudos, usando quer painéis de sondas direcionados quer abordagens de todo o genoma, permitiram identificar as regiões cromossómicas e genes mais comummente alterados. As técnicas de análise de todo o genoma revelaram novas regiões cromossómicas e genes que parecem desempenhar um papel no desenvolvimento e evolução clínica do CECP. No geral, através desta caraterização genómica, epigenética e trasncriptómica, identificámos biomarcadores e assinaturas moleculares de prognóstico e sobrevivência, o que abre novas portas para a medicina personalizada no CECP. Finalmente, utilizámos estas tecnologias de genómica e epigenética para caraterizar quatro doentes paradigmáticos com CECP de forma a provar o benefício deste conhecimento molecular na conduta clínica. Várias regiões cromossómicas e genes relacionados com a resistência à radiação, quimioterapia, prognóstico e sobrevivência foram identificados, o que poderia ajudar na escolha do tipo e intensidade das modalidades de tratamento. Adicionalmente, foi realizada a caraterização molecular de linhas comerciais de CECP e de culturas primárias estabelecidas a partir destes doentes de CECP, o que revelou a ploidia e rearranjos estruturais complexos destes tumores, garantindo modelos celulares para futuros estudos no campo da radiação e farmacogenómica e ainda para uma melhor compreensão dos mecanismos moleculares de desenvolvimento e progressão do CECP. Este trabalho permitiu, de uma forma robusta, caracterizar molecularmente o CECP, usando diferentes abordagens ómicas nas mesmas amostras tumorais, ajudando assim a identificar novos biomarcadores de prognóstico e assinaturas moleculares com potencial translação à clínica.
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Torres, Sara Cristina da Costa. "Cancro oral: perspetiva atual." Master's thesis, 2018. http://hdl.handle.net/10284/7423.
Full textAbstract:
O cancro oral é a 6º neoplasia mais comum em todo mundo e o carcinoma espino- celular representa 90% dos casos. A taxa de sobrevivência aos 5 anos é cerca de 50% devido ao estádio avançado do tumor no momento do diagnóstico. Esta realidade motiva uma intensa investigação no sentido de aprofundar o conhecimento do processo de carcinogénese bem como a procura de novas estratégias, alvo e esquemas terapêuticos.
A medicina dentária tem um papel fundamental, nomeadamente na prevenção, realização do diagnóstico precoce e, no encaminhamento dos pacientes para os centros de referência, bem como no acompanhamento dos doentes quer na fase prévia, durante e após os tratamentos.
O objetivo deste estudo foi realizar uma revisão narrativa da neoplasia oral avaliando os dados epidemiológicos do carcinoma da cavidade oral, factores de risco envolvidos, principais lesões potencialmente malignas, diagnóstico, tratamentos e demonstrar o importante papel do médico dentista neste processo.Oral cancer is the 6th most common neoplasm in the world and Squamous cell carcinoma represents 90% of the cases. The survival rate at 5 years old is about 50% due to the advanced stage of the tumor at the time of diagnosis. This reality motivates an intense investigation in the sense of improving the search of knowledge in the process of carcinogenesis as well as the search of new strategies, targets and therapeutic schemes. Dental medicine plays a key role, particularly in the prevention, doing the early diagnosis and referring the patients to specific centers as well as in their follow-up, both in the pre-treatment phase, during and after treatment. The objective of this study was to perform a narrative review of oral neoplasm by evaluating the epidemiological data of oral cavity carcinoma, risk factors involved, main potentially malignant lesions, diagnosis and treatments, as well as to demonstrate the important role of the dentist in this process.
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Martins, Beatriz Fernandes. "Avaliação do nível de conhecimento geral e grau de alerta sobre cancro oral numa população do nordeste transmontano." Master's thesis, 2016. http://hdl.handle.net/10284/5773.
Full textAbstract:
O cancro é um dos principais causadores de milhões de mortes em todo o mundo e
sendo o cancro oral, especificamente, a sexta neoplasia mais frequente a nível mundial.
Todos os anos são diagnosticados mais de 500 mil novos casos, sendo que as altas taxas
de mortalidade e mortalidade não se têm alterado ao longo dos anos.
A maior incidência de cancro oral encontra-se na Ásia e na Europa do Sul. Em Portugal,
mais precisamente em 2012, foram diagnosticados cerca de 1924 novos casos de cancro
oral, dos quais 967 ocorreram em homens.
O carcinoma espinocelular é o tipo histológico mais comum, sendo que 90% dos casos
de cancro oral são deste tipo. Sabe-se também que esta variante é mais frequente no
sexo masculino entre a 5ª e 6 ª década de vida apesar de, a incidência no sexo feminino,
ter vindo a aumentar, devido à contínua exposição ao tabaco, álcool e a outros factores
de risco.
Como foi dito anteriormente, o cancro oral tem uma alta taxa de mortalidade e de
morbilidade e, apesar dos avanços no diagnóstico, no tratamento e no conhecimento de
quais os factores de risco desta patologia, a taxa de sobrevivência ainda é inferior a 50%
o que revela que, o grande problema, passa pelo diagnóstico do cancro em estádios
avançados.
Assume-se então que, grande parte dos casos de cancro oral, poderiam ter sido evitados
se houvesse maior conhecimento e grau de alerta sobre a doença o que tendencialmente,
levaria a diagnósticos mais precoces.
Neste sentido, este estudo tem como propósito a avaliação do nível de conhecimento
geral e do grau de alerta de uma população do interior do país, mais precisamente do
Nordeste Transmontano, bem como, efectuar o registo da percepção dos inquiridos
relativamente a esta patologia, passando pelo reconhecimento da doença, pelo
conhecimento epidemiológico e etiológico, e pela melhor percepção a nível de sinais e
sintomas clínicos próprios desta patologia.Cancer ir a major cause of millions of deaths around the world, and oral cancer, specifically, is the sixth most common cancer worldwide. Each year 500,00 new cases are diagnosed, being a cancer associated with high rates of mortality and morbility. The highest incidence of oral cancer is in Asia and in Southern Europe. In Portugal, more precisely in 2012 were diagnosed about 1924 new cases of oral cancer, 967 occurred in men. As mentioned, oral cancer has a high mortality rate and poor prognosis, and despite advances in detection, treatment and knowledge of what are the risk factors of this disease, the survival rate is still below 50% which reveals that the major problem,remains in the diagnosis of cancer in advanced stages. It is assumed, that most cases of oral cancer could be prevented if there was greater awareness and knowledge about the disease which in turn would lead to earlier diagnosis. This study aims to evaluate the general level of knowledge and degree of alert of a specific population, more precisely from the northeast of Portugal and register the perception of this population regarding this pathology, through recognition of the disease, the epidemiological and etiological knowledge, and for better understanding the signs and symptoms of this condition.
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Chiu, I.-Wen, and 邱意彣. "Genome wide analysis of oral squamous cell carcinoma." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/23630161363930884402.
Full textAbstract:
碩士亞洲大學
生物科技學系碩士班
97
Oncogenes serve as specific and unique targets for drug intervention to treat cancer, as their activation (amplification and activating mutation) is essential for tumor development. Targeting of oncogenes has proven clinically useful in treating patients with HER2/neu gene amplification and BCR/Abl oncogene activation. Likewise, amplification of specific oncogenes could be linked with disease stage and response of chemotherapy, suggesting oncogene amplification as potential biomarker for outcome prediction. Given significant clinical impact, identification and characterization of new oncogenes promise to advance clinical management of cancer patients. Unfortunately, only a few such crucial oncogenes have been identified in oral cancer. We propose to identify new oncogenes by screening amplified chromosomal regions in oral squamous cell carcinoma, most common type of oral cancer, using a rational and comprehensive approach. Recent completion of human genome project together with advances in genome-wide DNA and transcriptome-wide RNA analyses provides unprecedented opportunity for comprehensive analysis of human cancer genome in fine detail. With these innovations as robust tools, we can now measure gene copy number changes in cancer quantitatively, plus map those changes directly onto a human genome. Candidate genes with both amplification and over-expression were then analyzed for potential somatic activating mutation, using a high throughput sequencing platform. We expect analysis of oral cancer genome and identification of new oncogenes not only to shed light on pathogenesis of oral cancer but also lay groundwork for new diagnostic tests and novel therapeutic intervention for this devastating disease.
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Liu, Yi-Ching, and 柳依青. "Expression of Gα12 in oral squamous cell carcinoma." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/52690522087536223361.
Full text
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Lin, Chiao-Ying, and 林佼穎. "Annexin A1 expression in oral squamous cell carcinoma." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/37879704074756447770.
Full textAbstract:
博士國立臺灣大學
臨床牙醫學研究所
96
To investigate whether annexin A1 (ANXA1) expression is a marker in predicting the prognosis of oral cancer patients. We immunohistochemically examined the expression of ANXA1 in 66 cases of oral epithelial dysplasia (OED) and 115 cases of oral squamous cell carcinoma (OSCC). The results were correlated with the clinicopathological parameters of tumors and overall patient survival. In normal oral mucosa, ANXA1 staining was predominantly located on the cell membrane. In OED and OSCC specimens, membranous staining decreased, whereas nuclear staining increased. Positive nuclear staining was observed in 9 of 66 (13.64%) OED cases and 63 of 115 (54.8%) OSCCs. Kaplan–Meier curves showed that OSCC patients with ANXA1 nuclear staining had significantly shorter overall lengths of survival (P¼0.00036 by the log-rank test). Multivariate analysis showed that ANXA1 nuclear staining is a significant predictor of poor overall survival. And oral cancer SAS cells treated with hepatocyte growth factor (HGF) can induce ANXA1 protein translocation from cytoplasm to nucleus. Cells pretreated with LY294002 (PI3K inhibitor) almost completely inhibited (88.3% inhibition) HGF-mediated ANXA1 nuclear translocation. The nuclear localization of ANXA1 protein is a frequent event and could be used as a prognostic factor in OSCC.
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Chong-YiHong and 洪崇翊. "DNA methylation deregulation in oral squamous cell carcinoma." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/2brd9b.
Full textAbstract:
碩士國立成功大學
口腔醫學研究所
104
Oral squamous cell carcinoma (oral cancer) was ranked the fifth of cancer incidence and increasing annually in Taiwan. The carcinogenesis of oral cancer is deeply investigated, revealed the cumulative carcinogenesis at the genetic and epigenetic levels, and also the feasible biomarker in oral cancer. Oncogene could be expressed or tumor suppression gene repressed as result of DNA methylation deregulated of gene promoter region. Pyrosequencing assay was used to evaluate the methylation level in oral cancer cell lines and patient tissue samples, and AQP5 was hypermethylation in oral cancer cell lines and precancer tissue samples. The expression of AQP5 was low by using qPCR. We concluded that the hypermethylation of AQP5 in precancer could induce low expression of AQP5, and hypomethylation of AQP5 in oral cancer might be the oncogene in oral cancer carcinogenesis.
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LAI, PEI-YU, and 賴佩妤. "Roles Of CD164 In Oral Squamous Cell Carcinoma." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/csue42.
Full textAbstract:
碩士國防醫學院
牙醫科學研究所
106
Cancer has ranked the number one cause of death in Taiwan. Oral cancer rank fourth of male’s cancer, which has the highest rate of increase. Cancer metastasis to distant site is the most common cause of death of patient. This malignant progression is enhanced by tumor cell activation of an epithelial to mesenchymal transition (EMT). Therefore, understanding the main regulatory mechanism of this malignancy is the key to develop novel and effective therapeutic strategies for oral squamous cell carcinoma (OSCC). CD164, a sialomucin, also known as endolyn or MGC-24, was a cell adhesion molecule which regulates proliferation, adhesion, and differentiation of hematopoietic stem cells. Emerging evidences indicated that the expression of CD164 was involved in the tumorigenesis and cancer progression in colon, prostate and ovarian cancers. However, few data were available regarding the clinical significance of CD164 signaling axis in oral cancer. The objective of this study was to investigate the role of CD164 in the tumorigenesis of the oral cancer. In this study, we test the hypothesis that CD164 is a critical factor for oral cancer metastasis and regulator of epithelial to mesenchymal transition. First we screen CD164 expression in a panel of oral cancer lines and the effect of CD164 on cell functions. Our preliminary results showed that CD164 expression is required to maintain the mesenchymal-like invasiveness of oral cancer cell. Knockdown of CD164 can attenuate oral cancer cell migration, proliferation and decrease CXCR4 expression. We also demonstrate that EMT markers were induced by CD164 modulation in oral tumor. These findings suggest that CD164 participated in the process of EMT that is required for oral cancer malignancy. In conclusion, our data show that CD164 contributes to EMT and regulates cell functions in oral cancer. Moreover, identification of CD164 as a cancer cell marker would possibly provide valuable approaches to develop effective therapy for oral cancer.
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WU, I.-CHEN, and 吳怡貞. "Roles of PIAS2 in Oral Squamous Cell Carcinoma." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/77726549103735439874.
Full textAbstract:
碩士國防醫學院
生物化學研究所
105
Oral cancer is the ranked fifth most frequently diagnosed cancer worldwide, as well as the ranked fifth frequent cause of cancer death in Taiwan. In spite of its convenience for diagnosis, prognosis is still poor. The crux of the matter in treatment lies in the biological heterogeneity of the tumor. There are no effective therapies to cure most of oral cancer patients. Gene variation contains various types including post-translational modification (PTM) which involves phosphorylation, methylation, ubiquitylation and SUMOylatoin. Based on our previous results, PIAS2, a SUMO E3-ligase, revealed abnormal expression in oral cancer patients. Roles of PIAS2 in cancers has not clearly defined yet. Therefore, we try to elucidate the relationship between PIAS2 and oral cancer. In this study, we first examined the expression of PIAS2 in oral cancer and normal cell lines. Furthermore, we knocked down PIAS2 by siRNA in oral cancer cell lines. Then the cell functions including of epithelial-mesenchymal transition (EMT), migration, invasion, proliferation and cell cycle were examined. First, we showed the upregulation of PIAS2 expression is noted in oral cancer compared to normal cells. Our results also revealed that knocked down PIAS2 in oral cancer cell increased epithelial markers expression along with reduced cell migration, invasion and proliferation ability. Moreover, it also influenced cell cycle progression. Thus our observations suggest that PIAS2 may act as oncogene in oral cancer. We further investigated potential mechanism of PIAS2 in oral cancer cell. Using immunoprecipitation assay, we demonstrated PIAS2 interact with P53 and P53 SUMOylatoin is depend on PIAS2. Additionally, our data indicated that PIAS2 also affects protein expression level of androgen receptor (AR). In conclusion, our data suggest that PIAS2 is closely related with oral cancer, which might affect P53 SUMOylation and AR expression. These results provide the role of PIAS2 in oral cancer and a new direction of its future application.
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Chen, Jia-Ni, and 陳佳妮. "Analyzing Genetic Variation in Oral Squamous Cell Carcinoma." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/46824453592107964093.
Full textAbstract:
碩士中國醫藥大學
生物科技學系碩士班
97
Oral Squamous Cell Carcinoma(OSCC) is the sixth of common death carcinoma in Taiwan. In the past, the research of genomic variation in OSCC is rare, so in the study we used a genome-wide array comparative genomic hybridization (aCGH) technology to assess 10 OSCC patients for detailed analysis of genomic aberration that may play a role in the development and progression of oral cancer. Our aCGH results revealed DNA copy number amplification on chromosome 1p, 3q, 7p, 8q, 9, 11q, 16, 17, 19, 20 and 22, as well as DNA copy number deletion on chromosome 3p, 4q, 5q, 8p, 10, 18q and Y. In particular, we found on WWP1, located at chromosome 8q21, which has been proposed to be an oncogene for breast cancer and prostate cancer. WWP1 is a ubiquitin ligase associated with the post-translational regulation of oncoproteins and tumor suppressor proteins. Our aCGH results revealed 80％ WWP1 DNA copy number amplification. In accordance with WWP1 copy number change and mRNA expression in OSCC clinical samples and cell lines, we applied Q-PCR technology to confirm our data. Our results showed that 39% of 59 OSCC clinical DNA samples represent WWP1 amplification, 35% of 46 OSCC clinical RNA samples represent WWP1 overexpression. To analyze WWP1 protein expression in clinical samples, we applied IHC technology to detect the level of WWP1 protein in 18 paired OSCC samples, our data represented different they had different protein pattern and location. In OSCC cell lines, WWP1 copy number change had different tendency with mRNA, protein expression. For the first time, we analyzed WWP1 in clinical OSCC samples and cell lines in this study, our data showed that WWP1 DNA amplification maybe associated with carcinogenesis of OSCC.