Relevant bibliographies by topics / SRB assay

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'SRB assay'

Author: Grafiati
Published: 4 June 2025
Last updated: 20 July 2025

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Journal articles on the topic "SRB assay"

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Christophersen, C. T., M. Morrison, and M. A. Conlon. "Overestimation of the Abundance of Sulfate-Reducing Bacteria in Human Feces by Quantitative PCR Targeting the Desulfovibrio 16S rRNA Gene." Applied and Environmental Microbiology 77, no. 10 (2011): 3544–46. http://dx.doi.org/10.1128/aem.02851-10.

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ABSTRACTThe dominant genus of sulfate-reducing bacteria (SRB) in humans isDesulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism.
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Sikander, Mohammed, Shabnam Malik, Anyssa Rodriguez, et al. "Abstract 399: Molecular mechanism of ormeloxifene mediated chemo-sensitivity in hepatocellular carcinoma." Cancer Research 83, no. 7_Supplement (2023): 399. http://dx.doi.org/10.1158/1538-7445.am2023-399.

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Abstract Background: Hepatocellular carcinoma (HCC) is a lethal cancer with a dismal 5-year survival rate as the standard therapy available Sorafenib (SRB), is only effective in extending survival for a subset of patients. Resistance to apoptosis is a defining characteristic of cancer cells and a crucial factor in cancer recurrence and relapse. Although, anticancer potential of the FDA-approved compound Ormeloxifene (ORM), which has well-defined pharmacokinetic and pharmacodynamic properties, has been explored in a variety of cancers, its efficacy in HCC has remained unknown. Therefore, the pu
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Soikkeli, Anne, Cristina Sempio, Ann Marie Kaukonen, Arto Urtti, Jouni Hirvonen, and Marjo Yliperttula. "Feasibility Evaluation of 3 Automated Cellular Drug Screening Assays on a Robotic Workstation." Journal of Biomolecular Screening 15, no. 1 (2009): 30–41. http://dx.doi.org/10.1177/1087057109352236.

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This study presents the implementation and optimization of 3 cell-based assays on a TECAN Genesis workstation—the Caspase-Glo® 3/7 and sulforhodamine B (SRB) screening assays and the mechanistic Caco-2 permeability protocol—and evaluates their feasibility for automation. During implementation, the dispensing speed to add drug solutions and fixative trichloroacetic acid and the aspiration speed to remove the supernatant immediately after fixation were optimized. Decontamination steps for cleaning the tips and pipetting tubing were also added. The automated Caspase-Glo® 3/7 screen was successful
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Nidugala, Hema, Ashwini Prabhu, Ramakrishna Avadhani, and Ravishankar B. "In vitro anticancer efficacy of Cyperus rotundus (L.) on breast adenocarcinoma cells via the induction of DNA fragmentation and apoptosis." Biomedicine 43, no. 4 (2023): 1198–202. http://dx.doi.org/10.51248/.v43i4.2309.

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Introduction and Aim: Cyperus rotundus (L.), commonly known as nutgrass, has been in use in Indian medicine traditional system against several ailments. This investigation was performed to assess the in vitro antineoplastic activity of extracts prepared from water and ethanol with C. rotundus rhizomes. Materials and Methods: Toxic effects induced by the C. rotundus extracts on MCF-7 breast adenocarcinoma cells was evaluated using Sulphorhodamine- B (SRB) Assay, Trypan blue assay, Hoechst nuclear staining assay, Acridine Orange-Ethidium bromide (AO-EB) differential fluorescence staining assay a
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dos Santos, Marcela Silva, Juliana da Silva, Ana Paula Simões Menezes, et al. "Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems." Oxidative Medicine and Cellular Longevity 2018 (August 19, 2018): 1–9. http://dx.doi.org/10.1155/2018/7804135.

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The use in folk medicine of Baccharis trimera and recent studies on DNA damage by oxidative stress mechanisms have motivated this study. We investigated the biotoxicological effects of trimeroside from this plant. Aqueous extract from aerial parts of B. trimera was fractioned by flash chromatography for further isolation by thin-layer chromatography. The novel nor-monoterpene glycoside, trimeroside, and three flavonoids, cirsimaritin, luteolin and quercetin, were isolated. The genotoxic and mutagenic potential of trimeroside was determined by Salmonella/microsome (TA98 and TA100), comet assay,
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Bianchi, E., A. Goldoni, L. Trintinaglia, et al. "Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil." Brazilian Journal of Biology 75, no. 2 suppl (2015): 68–74. http://dx.doi.org/10.1590/1519-6984.1913.

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<p>Some water bodies in the Sinos River Basin (SRB) have been suffering the effects of pollution by residential, industrial and agroindustrial wastewater. The presence of cytotoxic and genotoxic compounds could compromise the water quality and the balance of these ecosystems. In this context, the research aimed to evaluate the genotoxicity and cytotoxicity of the water at four sites along the SRB (in the cities of Santo Antônio da Patrulha, Parobé, Campo Bom and Esteio), using bioassays in fish and cell culture. Samples of surface water were collected and evaluated <italic>in vitro
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Randive, D. S., K. P. Shejawal, S. D. Bhinge, et al. "GREEN SYNTHESIS OF GOLD NANOPARTICLES OF ISOLATED CITRUS BIOFLAVONOID FROM ORANGE: CHARACTERIZATION AND IN VITRO CYTOTOXICITY AGAINST COLON CANCER CELL LINES COLO 320DM AND HT29." INDIAN DRUGS 57, no. 08 (2020): 61–69. http://dx.doi.org/10.53879/id.57.08.12514.

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The aim of the present research was to perform green synthesis of gold nanoparticles of isolated citrus bioflavonoid from Citrus sinensis (orange) peel extract and to evaluate its anticancer potential. Methanolic extract of orange peel was obtained by Soxhlet extraction and citrus bioflavonoid was isolated by using column chromatography. Gold nanoparticles were developed by green synthesis method, wherein 1 % aqueous solution of isolated citrus bioflavonoid were mixed with 1% solution of HAuCl4 and incubated at ambient temperature for 4 to 5 hours and observed for the color change which indica
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Khatun, Rafeza, Iffat Hasnin, Anamul Haque, and Md Aziz Abdur Rahman. "Comparative Cytotoxicity of Selected Mikania species using Brine Shrimp Lethality Bioassay and Sulforhodamine B (SRB) Assay." Bangladesh Pharmaceutical Journal 24, no. 1 (2021): 11–16. http://dx.doi.org/10.3329/bpj.v24i1.51630.

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This study aims to assess the comparative cytotoxic activity of the ethanolic extract of Mikania cordata (MC), Mikania micrantha (MM) and Mikania scandens (MS) (Family: Asteraceae) using brine shrimp lethality bioassay and colorimetric sulforhodamine B assay method. In SRB assay, A549 human lung carcinoma, SK-Mel-2 skin melanoma and B16F1 mouse melanoma cell lines were used. In brine shrimp assay, the LC50 for MC, MM, MS and vincristine were found to be 29.04, 15.84, 32.35, and 1.2 μg/ml, respectively. The results indicate that all the Mikania species showed moderate lethality against nauplii.
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., Srivalli Tripuramallu* and Suthakaran R. "SULFORHODAMINE B COLORIMETRIC ASSAY FOR CYTOTOXICITY SCREENING FOR SOME PYRAZOLE-QUINAZOLINE DERIVATIVES." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 12 (2018): 16606–11. https://doi.org/10.5281/zenodo.2443347.

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<em>The sulforhodamine B (SRB) assay is a most advanced cell density determination, with the measurement of cellular protein content. The method explained here has been optimized for the toxicity screening of compounds to adherent cells in a 96-well format. After an incubation period, cell monolayers are fixed with 10% (wt/vol) trichloroacetic acid and stained for 30 min, after which the excess dye is removed by washing repeatedly with 1% (vol/vol) acetic acid. The protein-bound dye is dissolved in 10 mM Tris base solution for OD determination at 510 nm using a microplate reader. The results a
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Wang, Zi, Wanjun Lin, Meina Shi та ін. "Involucrasin B Inhibits the Proliferation of Caco-2 Cells by Regulating the TGFβ/SMAD2-3-4 Pathway". Molecules 29, № 3 (2024): 686. http://dx.doi.org/10.3390/molecules29030686.

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(1) Background: Colorectal cancer (CRC) is the third most common malignant tumor worldwide and the second most common cause of cancer death. However, effective anti-CRC drugs are still lacking in clinical settings. This article investigated the anti-proliferative effect of involucrasin B on CRC Caco-2 cells. (2) Methods: This study employed a sulforhodamine B (SRB) method, colony formation experiments, flow cytometry, FastFUCCI assay, dual luciferase assay, and Western blot analysis for the investigation. (3) Results: The SRB method and colony formation experiments showed that involucrasin B e
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Dissertations / Theses on the topic "SRB assay"

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Silva, Júnior Flavio Manoel Rodrigues da. "Atividade mutagênica em solos sob a influência de rejeitos de carvão." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2008. http://hdl.handle.net/10183/13841.

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O solo é um compartimento ambiental altamente complexo e, em razão do crescimento populacional acelerado e da intensa atividade industrial e agrícola, tem sofrido com a contaminação de substâncias de origens diversas. Entre as principais atividades que contribuem para a perturbação da qualidade dos solos estão as atividades de geração de energia pela queima de combustíveis fósseis, como o carvão mineral. Dentre as substâncias presentes nos rejeitos do carvão queimado em usinas termelétricas estão compostos orgânicos e metais pesados que interagem com o material genético, produzindo mutações e
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Assad, Roberley Araújo. "Estudo da prevalência de Candida spp. e de outras variáveis relacionadas à estomatite protética em pacientes geriátricos institucionalizados / Roberley Araújo Assad ; orientador, Edvaldo Antonio Ribeiro Rosa." reponame:Biblioteca Digital de Teses e Dissertações da PUC_PR, 2004. http://www.biblioteca.pucpr.br/tede/tde_busca/arquivo.php?codArquivo=43.

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Dissertação (mestrado) - Pontifícia Universidade Católica do Paraná, Curitiba, 2004<br>Inclui bibliografias<br>Devido à influência de medicamentos e de doenças sistêmicas que agravam o estado da candidose em pacientes usuários de próteses totais, propôs-se, neste trabalho realizar a análise quantitativa das espécies mais freqüentes de Candida em pacientes com sina
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Carbone, Paulo Henrique Lage. ""Pesquisa de anticorpos dirigidos a antígenos de fase latente e lítica do herpesvírus humano tipo 8 (HHV-8): prevalência em populações sob risco epidemiológico e em população sadia de São Paulo"." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-04072003-142052/.

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O presente trabalho teve como objetivo otimizar ensaios sorológicos para serem utilizados na pesquisa de anticorpos dirigidos ao Herpesvírus humano tipo 8 (HHV-8) e com eles explorar grupos de risco para adquirir, transmitir e desenvolver doença relacionada à esta infecção, como o sarcoma de Kaposi (SK). Tomando como base a literatura disponível, as condições do laboratório e a experiência profissional acumulada na Seção de Imunologia do Instituto Adolfo Lutz de São Paulo, foram selecionados e utilizados os ensaios de imunofluorescência indireta (IFI) e Western blot (WB) para a pesquisa de ant
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Wortmann, Andreas. "In vitro and in vivo examination of the cell surface glycoprotein CDCP1." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/40975/1/Andreas_Wortmann_Thesis.pdf.

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A number of reports have demonstrated the importance of the CUB domaincontaining protein 1 (CDCP1) in facilitating cancer progression in animal models and the potential of this protein as a prognostic marker in several malignancies. CDCP1 facilitates metastasis formation in animal models by negatively regulating anoikis, a type of apoptosis triggered by the loss of attachment signalling from cell-cell contacts or cell-extra cellular matrix (ECM) contacts. Due to the important role CDCP1 plays in cancer progression in model systems, it is considered a potential drug target to prevent the metast
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Huang, Bo Yi, and 黃渤溢. "Development of an assay platform for screening Src kinase inhibitors using dot-blot assay." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/90642775013174705676.

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碩士<br>國立清華大學<br>分子與細胞生物研究所<br>104<br>Src tyrosine kinase, known as a proto-oncogene, participates in many signal pathways to regulate various cellular functions. Abnormal activation of Src is also involved in cancer progression. Autophosphorylation of Src plays a crucial role in its activation. Herein, I have developed an assay platform to screen Src tyrosine kinase inhibitors by analyzing the autophosphorylation of Src. The autophosphorylation reaction was performed in a 96-well format thermocycler and the autophosphorylation of Src was analyzed by dot-blot assay. In this assay platform, the
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Kasper, Amanda Clare. "I. Studies Towards the Synthesis of Manassantins A and B and Analogues II. Efforts Towards a FRET-Based Assay to Characterize Palmitoylation of Src Kinases III. Synthesis of Oxepanes via Organocatalytic Oxa-Michael Reactions." Diss., 2010. http://hdl.handle.net/10161/3069.

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<p>This dissertation focuses on three main projects that include aspects of natural product synthesis, synthetic methodology, as well as the development of a small molecular probe for the study of protein palmitoylation. This first project introduces studies towards the synthesis of manassantins A and B which are potent inhibitors of hypoxia-inducible factor 1 (HIF-1). In addition, the preparation of manassantin A analogues is described along with preliminary structure-activity relationship (SAR) studies providing further insight into the structural requirements for HIF-1 inhibitory activity.
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Books on the topic "SRB assay"

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Speeckaert, Marijn, and Jopis Delanghe. Assessment of renal function. Edited by Christopher G. Winearls. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199592548.003.0007.

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Glomerular filtration rate (GFR) can be measured as the clearance of exogenous or endogenous filtration markers. Practical formulas permit estimation of creatinine clearance or GFR without timed urine collections in many stable patients with CKD. Standardization of serum creatinine is important for all of these estimation methods and implementing traceability of the assays to the new global SRM 967 standard has led to changes in clinical decision-making criteria. Calibration to an IDMS reference produces a lowering of serum creatinine values by 10–30% for most methods. Serum creatinine concent
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Book chapters on the topic "SRB assay"

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Valentin, By Jean-Pierre, Derek Leishman, and Hugo Vargas. "Overview of the ICH E14/S7B: Questions and Answers." In Drug Discovery and Evaluation: Safety and Pharmacokinetic Assays. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-030-73317-9_147-1.

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Valentin, Jean-Pierre, Derek Leishman, and Hugo M. Vargas. "Overview of the ICH E14/S7B: Questions and Answers." In Drug Discovery and Evaluation: Safety and Pharmacokinetic Assays. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-35529-5_147.

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Das, Mrinmoy, Badal Bhattacharyya, D. Pujari, and Gautam Handique. "Faunal Composition of Scarab Beetles and Their Hosts in Assam." In Arthropod Diversity and Conservation in the Tropics and Sub-tropics. Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-1518-2_21.

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Kumar, C. Sathish, H. Harikrishnan, R. Charulatha, and V. Shanmugaiah. "Evaluation of Pseudomonas sp. VSMKU-4046 for Suppression of Sheath Blight (ShB) of Rice in Detached Leaf Assay." In Prospects in Bioscience: Addressing the Issues. Springer India, 2012. http://dx.doi.org/10.1007/978-81-322-0810-5_33.

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Poulard, Coralie, Juliette Rambaud, Muriel Le Romancer, and Laura Corbo. "Proximity Ligation Assay to Detect and Localize the Interactions of ERα with PI3-K and Src in Breast Cancer Cells and Tumor Samples." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1346-6_12.

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Everton, Gustavo Oliveira, Ana Paula Muniz Serejo, Mariana Oliveira Arruda, et al. "Antioxidant and anticancer activity in vitro of Syzygium aromaticum essential oil." In Open Science Research XIX. Editora Científica Digital, 2025. https://doi.org/10.37885/250319073.

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Objective: This study evaluated the in vitro antioxidant and anticancer activities of essential oil (EO) from Syzygium aromaticum (clove). Methods: Clove flower buds were collected in Maranhão, Brazil, and hydrodistillation was used for EO extraction. Antioxidant activity was assessed via ABTS, DPPH, and FRAP assays, while anticancer activity was tested against various human cancer cell lines (colon, lung, liver, prostate, neuroblastoma, etc.) using the SRB assay to determine IC50 values. The chemical composition was analyzed by GC-MS. Results: The EO demonstrated strong antioxidant activity, especially in the ABTS assay (IC50 = 0.73 mg/L). It displayed significant cytotoxicity against cancer cells, particularly colon (502713; IC50 = 6.27 mg/L), liver (HepG2; IC50 = 6.99 mg/L), and neuroblastoma (IMR-32; IC50 = 6.89 mg/L). GC-MS revealed eugenol (71.11%) as the primary compound, followed by β-caryophyllene (19.55%) and humulene (3.99%), indicating geographic influences on chemotypes. Conclusion: S. aromaticum EO shows potent antioxidant and anticancer effects in vitro, likely due to its high phenolic content. Further in vivo and clinical studies are needed to validate its therapeutic potential and safety. These findings underscore the significance of natural products in developing complementary therapies for oxidative stress-related diseases and cancer.
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Everton, Gustavo Oliveira, Ana Paula Muniz Serejo, Mariana Oliveira Arruda, et al. "Antioxidant and anticancer activity in vitro of Chenopodium ambrosioides L. essential oil." In Open Science Research XIX. Editora Científica Digital, 2025. https://doi.org/10.37885/250319071.

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Objective: This study evaluated the in vitro antioxidant and anticancer activities of essential oil (EO) from Chenopodium ambrosioides. Methods: Leaves were collected in Maranhão, Brazil, and the EO was extracted via hydrodistillation using a Clevenger apparatus. Antioxidant activity was assessed through the ABTS, DPPH, and FRAP assays. Anticancer activity was evaluated on various cancer cell lines, including colon, lung, liver, and prostate, using the SRB assay to determine the inhibitory concentration (IC50). The chemical composition was analyzed via gas chromatography-mass spectrometry (GC-MS) to identify main bioactive compounds. Results: The EO exhibited strong antioxidant activity, particularly in the ABTS assay (IC50 = 3.43 mg/L). For anticancer evaluation, it showed significant cytotoxicity in colon cells (IC50 = 13.18 mg/L), liver cells (IC50 = 14.07 mg/L), and neuroblastoma cells (IC50 = 13.95 mg/L). Chemical analysis revealed high levels of α-terpinene (56.33%), ascaridole (11.25%), and p-cymene (10.21%), compounds linked to antitumor effects. Variability in EO composition across regions indicated multiple chemotypes. Conclusion: The EO of C. ambrosioides shows promise as a natural antioxidant and anticancer agent, with notable in vitro efficacy attributed to its bioactive monoterpenes. Further in vivo and clinical studies are required to confirm its safety and therapeutic potential. These findings highlight the pharmacological potential of medicinal plants and underscore the need for standardized extraction and application methods.
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Paul, Antonio, Rajamony Chellammal Arun Dileep, and Noble Gracious. "Crossmatch and Histocompatibility." In Bridging the Gap and Saving Lives - A Comprehensive Guide to Organ Donation [Working Title]. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.1006348.

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Chronic kidney disease is a worldwide public health problem for which kidney transplantation results in an improved quality of life and social rehabilitation, and savings in overall healthcare costs. The human leukocyte antigen (HLA) system, also known as the major histocompatibility complex (MHC) in humans, plays a crucial role in the immune system. It is responsible for producing cell surface molecules that are specifically designed to convey antigenic peptides to the T-cell receptor (TCR) found on T cells. HLA determines whether a patient has already developed antibodies against the donor’s antigens. Various platforms for crossmatching include CDC Crossmatch (CDC CXM), Flow cytometric crossmatch (FCXM), Luminex-based DSA Lysate crossmatch, and Virtual Crossmatch (VXM). VXM is a procedure used to determine the compatibility between a potential organ donor and recipient without physically mixing their blood samples. VXM does not function as a standalone laboratory test; instead, it depends on the analysis and interpretation of prior data obtained from the recipient’s anti-HLA analysis using Single Antigen Bead (SAB) assays and donor HLA molecular type. Epitope-based matching is a novel method of histocompatibility matching that involves estimation of the eplet against the antibody in the recipient’s serum based on the positive reaction pattern observed in the SAB assay.
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Sarin, Hemant. "Molecular, Pharmacologic and Biochemical Aspects of Polycarbonate Plastic Bisphenol Exposures." In Pharmaceutical Science. IntechOpen, 2025. https://doi.org/10.5772/intechopen.115284.

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Bisphenol acetone was initially studied for potential application as an estrogen substitute, and since then, it is in industrial use as dually-substituted bifunctional epoxide in epoxy resins and as poly-BPA ester carbonate for its elasticity and advanced material bend properties. The bisphenols are lipophilic chemicals with hydrophilic substituents and steroid nucleus structure that can bind to cell surface and nuclear receptors to exert pituitary, thyroid and steroid axis, and adipose tissue / end organ-related health effects. The available bisphenols utilized in industry are known and have been characterized for their binding affinity by traditional assays such as saturation (-Ka) and competition (IC50), and reporter-based transfectant assay for study of their agonist (EC50) or antagonist (IC50) potential. The bisphenol A / BP receptors include the NR adapters, Ncor1, Smrt and Ncoa1-7 (Src), and the coadapter binding partners, Erα and Erβ, Gpr30 and Errγ, Rorα, Pparγ, and by effect on coadapter, TRα and TRβ. Dose-potency-efficacy concentration modeling is applicable to the study of NOAEL, LOAEL, ED50 and LD50 effects in population health and ecological studies. In this chapter, the sustentative knowledge on bisphenol properties, biochemical pathways, gene activation and repression, and epigenetics, is covered with meditation on cell-based receptor binding assays and potency-efficacy curvilinear relationships relevant to the epidemiology of exposure assessment.
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Goswami, Narayan Chandra, Parasmoni Dutta, Paula Richman, and Rustom Bharucha. "Questions around Rām Vijay." In Performing the Ramayana Tradition. Oxford University Press, 2021. http://dx.doi.org/10.1093/oso/9780197552506.003.0012.

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In conversation with Sri Narayan Chandra Goswami, the renowned sattradhikar (abbot) of the Natun Kamalabari Sattra in Assam, the co-editors of the volume, along with translator and annotator Parasmoni Dutta, raise questions relating to the history of the monastic Vaishnavite performance tradition Sattriya. Performed by monks as part of their devotional service in the sattras (monasteries) of Majuli in Assam, this tradition is attributed to the Assamese saint and social reformer, Sri Sankaradeva (1449–1568), whose play Rām Vijay is the only play in his oeuvre focusing on Ram through his marriage to Sita. Using the play as a catalyst for the discussion, Sri Narayan Chandra Goswami expounds on a range of issues—the socio-historical and cultural dimensions of Sattriya; its patronage by kings; its use as a popular means of proselytizing bhakti (a personalized devotional worship); and the representation of everyday life in the larger social and cultural context of the regional history of Assam.
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Conference papers on the topic "SRB assay"

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Zhu, Xiang Y., Alvin Ayala, Hetal Modi, and John J. Kilbane. "Application of Quantitative, Real-Time PCR in Monitoring Microbiologically-Influenced Corrosion (MIC) in Gas Pipelines." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05493.

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Abstract Quantitative PCR (qPCR) methods (fluorogenic 5′-nuclease assay and SYBR Green I assay) were developed to detect and quantify bacteria and some critical sub-populations related to gas and oil pipeline corrosion. 16S rRNA genes were targeted for the detection and quantification of total bacteria using fluorogenic 5′-nuclease assays. The functional genes, dissimilatory sulfite reductase gene (dsrAB), nitrite reductase gene (nirS), and methyl-coenzyme M reductase gene (mcrA), were targeted for sulfate reducing bacteria (SRB), denitrifying bacteria, and methanognes, respectively. The metho
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Tatnall, R. E., K. M. Stanton, and R. C. Ebersole. "Methods of Testing for the Presence of Sulfate-Reducing Bacteria." In CORROSION 1988. NACE International, 1988. https://doi.org/10.5006/c1988-88088.

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Abstract Experience with testing cooling water and oilfield produced water has shown that the common methods of testing for sulfate-reducing bacteria (SRB) can produce misleading results. Various methods of testing for and enumerating SRB are discussed, and the advantages and limitations of each are reviewed. These methods include broth bottles, agar deeps, melt agar tubes, adenosine triphosphate (ATP) assay, a specific (cell surface) antibody method, and a developmental specific antibody technique that tests for an enzyme unique to SRB. It is seen that no method is perfect and that the limita
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Eisenlord, S., A. Darzins, Carrie Keller-Schultz, and Victor V. Keasler. "Evaluation of Propidium Monoazide as a Tool to Differentiate Live from Dead MIC Microorganisms." In CORROSION 2016. NACE International, 2016. https://doi.org/10.5006/c2016-07347.

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Abstract We describe the advancement of an activity-based qPCR assay which can distinguish live from dead corrosion influencing microorganisms in oil and gas pipeline environments. While the current body of knowledge is expanding for the types of microbial organisms present in waters used for natural gas and oil production, there is scant information on the activity of these potentially deleterious or beneficial organisms in source waters and produced waters associated with the industry. This assay was developed using pure cultures of sulfate reducing bacteria (SRB), sulfate reducing Archaea (
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Liduino, Vitor Silva, Magali Christe Cammarota, and Eliana Flávia Camporese Sérvulo. "Electrochemically Active Biofilm on AISI 1020 Steel and Its Corrosion Potential." In LatinCORR 2023. AMPP, 2023. https://doi.org/10.5006/lac23-20888.

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Electroactive biofilms provide environments conducive to the occurrence of microbiologically influenced corrosion (MIC) in several industrial sectors. There are many distinct MIC mechanisms associated with different microbial groups and metabolism types. Most published research studies on MIC focus on bacteria, where sulfate-reducing bacteria (SRB) have the largest impact on corrosion because sulfate is widely distributed in anoxic environments, such as offshore oil reservoirs. This study investigated the role of an oilfield SRB-consortium in the AISI 1020 carbon steel corrosion immersed in hy
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Nemati, M., and G. Voordouw. "Identification and Characterization of Sulfate-Reducing Bacteria Involved in Microbially Influenced Corrosion in Oil Fields." In CORROSION 2000. NACE International, 2000. https://doi.org/10.5006/c2000-00126.

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Abstract Sulfate-reducing bacteria (SRB) are thought to be involved in microbially influenced corrosion (MIC) of oil field pipelines and equipment. Analysis of microbial communities on corrosion coupons installed in production facilities at Wainwright and Wildmere, two oil fields in Alberta, was done by reverse sample genome probing (RSGP) a DNA hybridization assay in which multiple bacteria can be tracked simultaneously. RSGP indicated dominance of SRB in the microbial communities present on 10 of 15 coupon samples. Of these Desulfovibrio spp. Lac6 and Eth3 were found to be resistant to cocod
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Leleika, Scott, Amanda Harmon, and Sarah Eisenlord. "Quantifying Sulfur Oxidizing Bacteria Using qPCR." In CORROSION 2018. NACE International, 2018. https://doi.org/10.5006/c2018-10902.

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Abstract The understanding of microbiologically influenced corrosion (MIC), specifically the role different species of microorganisms play in the process, is continuously evolving. Recently, bacteria beyond typically implicated sulfate reducing bacteria (SRB) and acid producing bacteria (APB) have been found to influence corrosion. Sulfur oxidizing bacteria (SOB) populations, for example, have been linked to the corrosion of concrete and steel. As with the other MIC organisms found in gas industry samples, modern molecular techniques targeting functional gene DNA are the most accurate ways to
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Fichter, Jennifer, Geddy Hamblen, Chris Janes, Elizabeth J. Summer, Elizabeth J. Summer, and Abigail Mills. "Use of a Methodological Panel to Identify the Source of Problematic Microbial Contamination in an Oil Shale Field." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09019.

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Abstract An oil shale field was found to exhibit classic signs of a heavy microbial burden, including incidences of hydrogen sulfide production, downhole and surface microbially influenced corrosion, downhole pump and surface equipment fouling and fracturing fluid and drilling mud degradation. Over 140 samples, including formation core material, drilling muds, fracturing fluid source waters, production well samples, samples collected from failed pipe surfaces and samples from salt water disposal facilities, were collected in a comprehensive survey. Microbial activity was measured in parallel u
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de Romero, Matilde F., Reinaldo Ortiz, Olga Pérez de Márquez, and María E. Romero-González. "Use of Ftir in Situ Technique to Evaluate MIC by SRB." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07531.

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Abstract Infrared Spectroscopy Technique based on diffuse, internal and external reflectance allows both, the performance of a general qualitative analysis of the structure and the identification of organic and inorganic compounds, through the excitation of molecular vibrations by light absorption. Through the technique, vibrational data is obtained from the surface film, determining the nature of the molecules adsorbed on the metallic surface. Different researchers have given their attention and efforts to the study of the interfase region, by using Fourier Transformed Infrared Spectroscopy (
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Grigoryan, Alexander. "Challenges in Microbial Monitoring of an Oil-water Separation Facility." In MECC 2023. AMPP, 2023. https://doi.org/10.5006/mecc2023-20123.

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Abstract Abundance and diversity of microorganisms, including H2S-producing and corrosive bacteria, in the produced water collected from different locations at a Central Arabian Gas-Oil Separation Plant (GOSP) were examined by culture-based and culture-independent methods. Conventional MPN techniques showed that abundance of general heterotrophic bacteria exceeded acceptable levels more frequently in water samples from High Pressure-Production Trap (HPPT) and Water-Oil Separator (WOSEP) than in water from Production Header (PH). MPN assay of corrosive sulfidogenic sulfate-reducing bacteria (SR
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Trivedi, Rishi Raj, Olivia Stenzel, Seth Sheridan, and Marcelo Filgueira. "Biocide Stability and Efficacy in Soured Oilfield Environments." In CORROSION 2019. NACE International, 2019. https://doi.org/10.5006/c2019-13163.

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Absrract One of the most problematic and costly microbial issues of the oil and gas industry is biotic reservoir souring. Reservoir souring can be minimized by proper treatment of injection water to control sulfate-reducing bacteria (SRB). However, control of this process is often lost due to unintended interaction of the biocidal active species with hydrogen sulfide resulting in the deleterious production of H2S in production wells. This study investigates the stability and efficacy of commonly used oil and gas biocides in a soured environment to determine their suitability for use in souring
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Reports on the topic "SRB assay"

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Tobin, Stephen Joseph, Michael Lynn Fugate, Holly Renee Trellue, et al. Nondestructive Assay Data Integration with the SKB-50 Assemblies - FY16 Update. Office of Scientific and Technical Information (OSTI), 2016. http://dx.doi.org/10.2172/1330638.

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Griffin, G. D., M. R. Baker, and I. Sauers. Simple chemical assays for presence of S{sub 2}F{sub 10} in decomposed SF{sub 6}. Office of Scientific and Technical Information (OSTI), 1994. http://dx.doi.org/10.2172/10189263.

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Holland, M., and S. Sheldon Nichols. EVALUATING EFFECTS OF NEPTUNIUM ON THE SRS METHOD FOR CONTROLLED POTENTIAL COULOMETRIC ASSAY OF PLUTONIUM IN SULFURIC ACID SUPPORTING ELECTROLYTE. Office of Scientific and Technical Information (OSTI), 2008. http://dx.doi.org/10.2172/929466.

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CASELLA, VITO. TASK TECHNICAL AND QUALITY ASSURANCE PLAN FOR GAMMA ASSAYS AT THE SRS SOLID WASTE MANAGEMENT FACILITIES. Office of Scientific and Technical Information (OSTI), 2006. http://dx.doi.org/10.2172/882702.

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Guram, M. S. Development of critical life stage assays: Teratogenic effects of SRS effluent components on freshwater fish, gambusia. Office of Scientific and Technical Information (OSTI), 1990. http://dx.doi.org/10.2172/6225646.

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Avni, Adi, and Gitta L. Coaker. Proteomic investigation of a tomato receptor like protein recognizing fungal pathogens. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7600030.bard.

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Maximizing food production with minimal negative effects on the environment remains a long-term challenge for sustainable food production. Microbial pathogens cause devastating diseases, minimizing crop losses by controlling plant diseases can contribute significantly to this goal. All plants possess an innate immune system that is activated after recognition of microbial-derived molecules. The fungal protein Eix induces defense responses in tomato and tobacco. Plants recognize Eix through a leucine-rich-repeat receptor- like-protein (LRR-RLP) termed LeEix. Despite the knowledge obtained from
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Levy, Maggie, Raymond Zielinski, and Anireddy S. Reddy. IQD1 Function in Defense Responses. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7699842.bard.

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The main objective of the proposed research was to study IQD1's mechanism of action and elucidate its role in plant protection. Preliminary experiments suggest that IQD1 binds CaM in a Ca²⁺-dependent manner and functions in general defense responses. We propose to identify proteins and genes that interact with IQD1, which may provide some clues to its mechanism of action. We also plan to dissect IQD1's integration in defense pathways and to study and modulate its binding affinity to CaM in order to enhance crop resistance. Our specific objectives were: (1) Analysis of IQD1's CaM-binding proper
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Jander, Georg, and Daniel Chamovitz. Investigation of growth regulation by maize benzoxazinoid breakdown products. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7600031.bard.

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Introduction Previous research had suggested that benzoxazinoids, a class of defensive metabolites found in maize, wheat, rye, and wild barley, are not only direct insect deterrents, but also influence other areas of plant metabolism. In particular, the benzoxazinoid 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxa- zin-3(4H)- one (DIMBOA) was implicated in: (i) altering plant growth by interfering with auxin signaling, and (ii) leading to the induction of gene expression changes and secondary plant defense responses. The overall goal of this proposal was to identify mechanisms by which benzoxazinoids i
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ASSAf Distinguished Visiting Scholar (DVS) Programme 2024/25. Academy of Science of South Africa (ASSAf), 2025. https://doi.org/10.17159/assaf.2025/112.

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The Academy of Science of South Africa (ASSAf) in partnership with Prof Caradee Wright (as National Host) from the South African Medical Research Council (SAMRC), hosted Prof Kalpana Balakrishnan on 03 - 14 March 2025 for the 2024/25 DVS Programme. Prof Balakrishnan delivered a series of lectures and trainings under the theme “Air Pollution, Climate and Human Health”, with emphasis on health equity considerations in low and middle income countries (LMICs). The itinerary of her visit spun across various institutions in three Provinces: University of Johannesburg (UJ), University of Pretoria (UP
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