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Journal articles on the topic 'SRB assay'

Author: Grafiati

Published: 4 June 2025

Last updated: 20 July 2025

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1

Christophersen, C. T., M. Morrison, and M. A. Conlon. "Overestimation of the Abundance of Sulfate-Reducing Bacteria in Human Feces by Quantitative PCR Targeting the Desulfovibrio 16S rRNA Gene." Applied and Environmental Microbiology 77, no. 10 (2011): 3544–46. http://dx.doi.org/10.1128/aem.02851-10.

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ABSTRACTThe dominant genus of sulfate-reducing bacteria (SRB) in humans isDesulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism.

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Sikander, Mohammed, Shabnam Malik, Anyssa Rodriguez, et al. "Abstract 399: Molecular mechanism of ormeloxifene mediated chemo-sensitivity in hepatocellular carcinoma." Cancer Research 83, no. 7_Supplement (2023): 399. http://dx.doi.org/10.1158/1538-7445.am2023-399.

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Abstract Background: Hepatocellular carcinoma (HCC) is a lethal cancer with a dismal 5-year survival rate as the standard therapy available Sorafenib (SRB), is only effective in extending survival for a subset of patients. Resistance to apoptosis is a defining characteristic of cancer cells and a crucial factor in cancer recurrence and relapse. Although, anticancer potential of the FDA-approved compound Ormeloxifene (ORM), which has well-defined pharmacokinetic and pharmacodynamic properties, has been explored in a variety of cancers, its efficacy in HCC has remained unknown. Therefore, the pu

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Soikkeli, Anne, Cristina Sempio, Ann Marie Kaukonen, Arto Urtti, Jouni Hirvonen, and Marjo Yliperttula. "Feasibility Evaluation of 3 Automated Cellular Drug Screening Assays on a Robotic Workstation." Journal of Biomolecular Screening 15, no. 1 (2009): 30–41. http://dx.doi.org/10.1177/1087057109352236.

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This study presents the implementation and optimization of 3 cell-based assays on a TECAN Genesis workstation—the Caspase-Glo® 3/7 and sulforhodamine B (SRB) screening assays and the mechanistic Caco-2 permeability protocol—and evaluates their feasibility for automation. During implementation, the dispensing speed to add drug solutions and fixative trichloroacetic acid and the aspiration speed to remove the supernatant immediately after fixation were optimized. Decontamination steps for cleaning the tips and pipetting tubing were also added. The automated Caspase-Glo® 3/7 screen was successful

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Nidugala, Hema, Ashwini Prabhu, Ramakrishna Avadhani, and Ravishankar B. "In vitro anticancer efficacy of Cyperus rotundus (L.) on breast adenocarcinoma cells via the induction of DNA fragmentation and apoptosis." Biomedicine 43, no. 4 (2023): 1198–202. http://dx.doi.org/10.51248/.v43i4.2309.

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Introduction and Aim: Cyperus rotundus (L.), commonly known as nutgrass, has been in use in Indian medicine traditional system against several ailments. This investigation was performed to assess the in vitro antineoplastic activity of extracts prepared from water and ethanol with C. rotundus rhizomes. Materials and Methods: Toxic effects induced by the C. rotundus extracts on MCF-7 breast adenocarcinoma cells was evaluated using Sulphorhodamine- B (SRB) Assay, Trypan blue assay, Hoechst nuclear staining assay, Acridine Orange-Ethidium bromide (AO-EB) differential fluorescence staining assay a

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dos Santos, Marcela Silva, Juliana da Silva, Ana Paula Simões Menezes, et al. "Biotoxicological Analyses of Trimeroside from Baccharis trimera Using a Battery of In Vitro Test Systems." Oxidative Medicine and Cellular Longevity 2018 (August 19, 2018): 1–9. http://dx.doi.org/10.1155/2018/7804135.

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The use in folk medicine of Baccharis trimera and recent studies on DNA damage by oxidative stress mechanisms have motivated this study. We investigated the biotoxicological effects of trimeroside from this plant. Aqueous extract from aerial parts of B. trimera was fractioned by flash chromatography for further isolation by thin-layer chromatography. The novel nor-monoterpene glycoside, trimeroside, and three flavonoids, cirsimaritin, luteolin and quercetin, were isolated. The genotoxic and mutagenic potential of trimeroside was determined by Salmonella/microsome (TA98 and TA100), comet assay,

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Bianchi, E., A. Goldoni, L. Trintinaglia, et al. "Evaluation of genotoxicity and cytotoxicity of water samples from the Sinos River Basin, southern Brazil." Brazilian Journal of Biology 75, no. 2 suppl (2015): 68–74. http://dx.doi.org/10.1590/1519-6984.1913.

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<p>Some water bodies in the Sinos River Basin (SRB) have been suffering the effects of pollution by residential, industrial and agroindustrial wastewater. The presence of cytotoxic and genotoxic compounds could compromise the water quality and the balance of these ecosystems. In this context, the research aimed to evaluate the genotoxicity and cytotoxicity of the water at four sites along the SRB (in the cities of Santo Antônio da Patrulha, Parobé, Campo Bom and Esteio), using bioassays in fish and cell culture. Samples of surface water were collected and evaluated <italic>in vitro

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Randive, D. S., K. P. Shejawal, S. D. Bhinge, et al. "GREEN SYNTHESIS OF GOLD NANOPARTICLES OF ISOLATED CITRUS BIOFLAVONOID FROM ORANGE: CHARACTERIZATION AND IN VITRO CYTOTOXICITY AGAINST COLON CANCER CELL LINES COLO 320DM AND HT29." INDIAN DRUGS 57, no. 08 (2020): 61–69. http://dx.doi.org/10.53879/id.57.08.12514.

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The aim of the present research was to perform green synthesis of gold nanoparticles of isolated citrus bioflavonoid from Citrus sinensis (orange) peel extract and to evaluate its anticancer potential. Methanolic extract of orange peel was obtained by Soxhlet extraction and citrus bioflavonoid was isolated by using column chromatography. Gold nanoparticles were developed by green synthesis method, wherein 1 % aqueous solution of isolated citrus bioflavonoid were mixed with 1% solution of HAuCl4 and incubated at ambient temperature for 4 to 5 hours and observed for the color change which indica

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Khatun, Rafeza, Iffat Hasnin, Anamul Haque, and Md Aziz Abdur Rahman. "Comparative Cytotoxicity of Selected Mikania species using Brine Shrimp Lethality Bioassay and Sulforhodamine B (SRB) Assay." Bangladesh Pharmaceutical Journal 24, no. 1 (2021): 11–16. http://dx.doi.org/10.3329/bpj.v24i1.51630.

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This study aims to assess the comparative cytotoxic activity of the ethanolic extract of Mikania cordata (MC), Mikania micrantha (MM) and Mikania scandens (MS) (Family: Asteraceae) using brine shrimp lethality bioassay and colorimetric sulforhodamine B assay method. In SRB assay, A549 human lung carcinoma, SK-Mel-2 skin melanoma and B16F1 mouse melanoma cell lines were used. In brine shrimp assay, the LC50 for MC, MM, MS and vincristine were found to be 29.04, 15.84, 32.35, and 1.2 μg/ml, respectively. The results indicate that all the Mikania species showed moderate lethality against nauplii.

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., Srivalli Tripuramallu* and Suthakaran R. "SULFORHODAMINE B COLORIMETRIC ASSAY FOR CYTOTOXICITY SCREENING FOR SOME PYRAZOLE-QUINAZOLINE DERIVATIVES." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 12 (2018): 16606–11. https://doi.org/10.5281/zenodo.2443347.

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<em>The sulforhodamine B (SRB) assay is a most advanced cell density determination, with the measurement of cellular protein content. The method explained here has been optimized for the toxicity screening of compounds to adherent cells in a 96-well format. After an incubation period, cell monolayers are fixed with 10% (wt/vol) trichloroacetic acid and stained for 30 min, after which the excess dye is removed by washing repeatedly with 1% (vol/vol) acetic acid. The protein-bound dye is dissolved in 10 mM Tris base solution for OD determination at 510 nm using a microplate reader. The results a

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Wang, Zi, Wanjun Lin, Meina Shi та ін. "Involucrasin B Inhibits the Proliferation of Caco-2 Cells by Regulating the TGFβ/SMAD2-3-4 Pathway". Molecules 29, № 3 (2024): 686. http://dx.doi.org/10.3390/molecules29030686.

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(1) Background: Colorectal cancer (CRC) is the third most common malignant tumor worldwide and the second most common cause of cancer death. However, effective anti-CRC drugs are still lacking in clinical settings. This article investigated the anti-proliferative effect of involucrasin B on CRC Caco-2 cells. (2) Methods: This study employed a sulforhodamine B (SRB) method, colony formation experiments, flow cytometry, FastFUCCI assay, dual luciferase assay, and Western blot analysis for the investigation. (3) Results: The SRB method and colony formation experiments showed that involucrasin B e

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AMIT, ONKARI, PARULBEN D. MEHTA, and SHARMA KAJAL. ""Development, Characterization and Anti-Cancer Potential of 5-Fluorouracil Loaded Folate Appended Gellan Gum Nanoparticles for Breast Cancer Targeting"." International Journal of Multidisciplinary Research and Studies 07, no. 06 (2024): 27–39. https://doi.org/10.5281/zenodo.11637126.

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This study explores the pharmacological investigation and anti-cancer potential of folate-modified Gellan gum nanoparticles, focusing on their impact on cancer cells through p53 activation and the inhibition of the mTOR/PI3K pathway. The nanoparticles were meticulously characterized using advanced techniques, including Nuclear Magnetic Resonance (NMR) and Fourier-Transform Infrared Spectroscopy (FTIR) to ensure structural integrity. Additionally, drug release kinetics, particle size, zeta potential, and entrapment efficiency were assessed to understand the formulation's physicochemical propert

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Moon, So-Jeong, Hyung-Jun Choi, Young-Hyeon Kye, et al. "CTTN Overexpression Confers Cancer Stem Cell-like Properties and Trastuzumab Resistance via DKK-1/WNT Signaling in HER2 Positive Breast Cancer." Cancers 15, no. 4 (2023): 1168. http://dx.doi.org/10.3390/cancers15041168.

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Background: Despite the therapeutic success of trastuzumab, HER2 positive (HER2+) breast cancer patients continue to face significant difficulties due to innate or acquired drug resistance. In this study we explored the potential role of CTTN in inducing trastuzumab resistance of HER2+ breast cancers. Methods: Genetic changes of CTTN and survival of HER2+ breast cancer patients were analyzed in multiple breast cancer patient cohorts (METABRIC, TCGA, Kaplan-Meier (KM) plotter, and Hanyang University cohort). The effect of CTTN on cancer stem cell activity was assessed using the tumorsphere form

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Sudha, T., D. Mahalakshmi, and P. Kumar Nallasivan. "Design, Synthesis, Characterization, Molecular Docking Studies, In vitro and In vivo Cervical Cancer Activity of Novel N-Substituted Pyrazole Derivatives." Asian Journal of Chemistry 36, no. 10 (2024): 2260–68. http://dx.doi.org/10.14233/ajchem.2024.32021.

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The aim of this study is to design, synthesize, characterize few novel N-substituted pyrazole derivatives and evaluated for their anticancer capabilities; these compounds were selected based on their ability to inhibit the HPV E6 protein. The IR, 1H & 13C NMR, mass spectral and elemental analysis were used to determine the structural details of the newly synthesized substances. The molecular docking was performed using Auto Dock Vina, and the protein-ligand interaction was analyzed using Discovery Studio. The sulforhodamine B (SRB) assay was used to determine the in vitro anticancer activi

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Rodrigues, Elsa, Miguel Pardal, Vincent Laizé, M. Leonor Cancela, Paulo Oliveira, and Teresa Serafim. "Cardiomyocyte H9c2 cells present a valuable alternative to fish lethal testing for azoxystrobin." Environmental Pollution 206, November 2015 (2015): 619–26. https://doi.org/10.1016/j.envpol.2015.08.026.

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Beegum, Fathima, Anuranjana P V, Krupa thankam George, et al. "Investigating the role of sesamol in promoting the healing of diabetic wounds by analyzing molecular expression patterns in human diabetic dermal fibroblasts." Journal of Applied Pharmaceutical Research 12, no. 3 (2024): 107–18. http://dx.doi.org/10.69857/joapr.v12i3.505.

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Background: Sesamol (3,4-methylenedioxyphenol) is one of the plant compounds tested in vivo for diabetic wound healing, normal wound healing, and dexamethasone-induced delayed wound healing. Elucidation of mechanisms underlying the wound healing effect of sesamol through modulation of various molecular and cellular pathways is the crux of this paper. Objectives: The objective of the current work was to uncover the mechanism of sesamol underlying the treatment of diabetic wounds using gene expression analysis. Methods: The cytotoxicity assay was performed using an SRB colorimetric assay, from w

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Mishra, Sagar K., Sribatsa L. Dash, and Ranjit Mohapatra. "In-vitro Anticancer Activity of Various Plant Extracts." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 14, no. 01 (2023): 61–65. http://dx.doi.org/10.25258/ijpqa.14.1.11.

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Herbal medicinal plants have been explored for a variety of pharmacological properties but still a large number of phytoconstituents are still unexplored. The manuscript aims to assess the anticancer property of Leucas cephalotes (Roth) Spreng, Acalypha indica L. and Lantana camara L. extracts on MDA-MB-231, A-549, PC-3 and Hep-G2 cell lines. The ethanol extract of the selected plants was explored for anticancer activity by SRB assay. Since L. camara exhibited promising activity, this plant’s successive extraction in diff erent extracts (n-hexane, chloroform, ethanol and hydroalcoholic) was fu

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Farhat, Zeinab, Tyler Scheving, Diana S. Aga, et al. "Antioxidant and Antiproliferative Activities of Several Garlic Forms." Nutrients 15, no. 19 (2023): 4099. http://dx.doi.org/10.3390/nu15194099.

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It is hypothesized that garlic, Allium sativum, might protect against oxidative stress that causes damage to cells and tissues leading to the development of various health conditions including cancer. However, it is not known whether garlic’s potential anticancer benefits differ by form of garlic consumed. This study aimed to quantify and compare the in vitro antioxidant and antiproliferative activity of several garlic forms in water and alcohol extracts including fresh garlic, fresh garlic set aside, heated garlic, heated garlic set aside, garlic powder, black garlic, two commercially availab

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Kabała-Dzik, Agata, Anna Rzepecka-Stojko, Robert Kubina, Robert Dariusz Wojtyczka, Ewa Buszman, and Jerzy Stojko. "Caffeic Acid Versus Caffeic Acid Phenethyl Ester in the Treatment of Breast Cancer MCF-7 Cells: Migration Rate Inhibition." Integrative Cancer Therapies 17, no. 4 (2018): 1247–59. http://dx.doi.org/10.1177/1534735418801521.

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Epithelium mammary carcinoma is a cancer with a high death rate among women. One factor having a significant impact on metastasis is cell migration. The aim of this study was to compare migration rate inhibition of caffeic acid (CA) and its phenethyl ester (CAPE) on MCF-7 breast cancer cells. Microscopic evaluation was used to determine the morphology of carcinoma cells, before and after 24-hour treatment with CA and CAPE using a dose of 50 µM. The cytotoxic effect was measured by XTT-NR-SRB assay (tetrazolium hydroxide-neutral red-Sulforhodamine B) for 24-hour and 48-hour periods, using CA an

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Moghadam, Ebrahim S., Maryam H. Tehrani, René Csuk, et al. "2,4-Disubstituted Quinazoline Derivatives Act as Inducers of Tubulin Polymerization: Synthesis and Cytotoxicity." Anti-Cancer Agents in Medicinal Chemistry 19, no. 8 (2019): 1048–57. http://dx.doi.org/10.2174/1871520619666190314125254.

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Background: During last recent years number of anti-tubulin agents were introduced for treatment of diverse kind of cancer. Despite of their potential in treatment of cancer, drug resistance and adverse toxicity such as peripheral neuropathy are some of the negative criteria of anti-tubulin agents. Methods: Twenty seven quinazoline derivatives were synthesized using a multicomponent reaction. The cytotoxicity of compounds 1-27 was tested in SRB assays employing five different human tumor cell lines. Effect of two of active compounds on tubulin polymerization was also checked using a commercial

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Abeysinghe, Nuwanka K., Ira Thabrew, Sameera R. Samarakoon, et al. "Vernolactone Promotes Apoptosis and Autophagy in Human Teratocarcinomal (NTERA-2) Cancer Stem-Like Cells." Stem Cells International 2019 (December 19, 2019): 1–12. http://dx.doi.org/10.1155/2019/6907893.

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Vernonia zeylanica, is a shrub endemic to Sri Lanka. V. zeylanica has been used in Sri Lankan traditional medicine for the treatment of various diseases and conditions. The present study was designed to determine antiproliferative, apoptotic, autophagic, and antioxidant effects of vernolactone, isolated from V. zeylanica, in human embryonal carcinoma cells (NTERA-2, a cancer stem cell model). Antiproliferative effects of vernolactone in NTERA-2 cells and human peripheral blood mononuclear cells (control cells) were evaluated using the Sulforhodamine B (SRB) assay and WST-1 antiproliferative as

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Tavares-da-Silva, Edgar, Eurico Pereira, Ana S. Pires, et al. "Cold Atmospheric Plasma, a Novel Approach against Bladder Cancer, with Higher Sensitivity for the High-Grade Cell Line." Biology 10, no. 1 (2021): 41. http://dx.doi.org/10.3390/biology10010041.

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Antitumor therapies based on Cold Atmospheric Plasma (CAP) are an emerging medical field. In this work, we evaluated CAP effects on bladder cancer. Two bladder cancer cell lines were used, HT-1376 (stage III) and TCCSUP (stage IV). Cell proliferation assays were performed evaluating metabolic activity (MTT assay) and protein content (SRB assay). Cell viability, cell cycle, and mitochondrial membrane potential (Δψm) were assessed using flow cytometry. Reactive oxygen and nitrogen species (RONS) and reduced glutathione (GSH) were evaluated by fluorescence. The assays were carried out with differ

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Tavares-da-Silva, Edgar, Eurico Pereira, Ana S. Pires, et al. "Cold Atmospheric Plasma, a Novel Approach against Bladder Cancer, with Higher Sensitivity for the High-Grade Cell Line." Biology 10, no. 1 (2021): 41. http://dx.doi.org/10.3390/biology10010041.

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Antitumor therapies based on Cold Atmospheric Plasma (CAP) are an emerging medical field. In this work, we evaluated CAP effects on bladder cancer. Two bladder cancer cell lines were used, HT-1376 (stage III) and TCCSUP (stage IV). Cell proliferation assays were performed evaluating metabolic activity (MTT assay) and protein content (SRB assay). Cell viability, cell cycle, and mitochondrial membrane potential (Δψm) were assessed using flow cytometry. Reactive oxygen and nitrogen species (RONS) and reduced glutathione (GSH) were evaluated by fluorescence. The assays were carried out with differ

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Lewandowski, Łukasz, Iwona Bednarz-Misa, Alicja Kucharska, et al. "Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays." Molecules 27, no. 13 (2022): 4193. http://dx.doi.org/10.3390/molecules27134193.

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Despite the fact that phytochemicals of Cornaceae species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on Cornus mas L. (Cornelian cherry), the extracts of which are reported to exert a pleiotropic effect shown in both in vivo and in vitro studies. This study aimed to explore the cytotoxic effect of extracts from fruits of red (Cornus mas L. ‘Podolski’) and yellow (Cornus mas L. ‘Yantarnyi’ and ‘Flava’) Cornelian cherries on two melanoma cell lines (A375 and MeWo). The extracts wer

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Sharma, Ankush, Varsha R. Solanki, and Mohamed Muzammel. "In vitro anticancer activity of Bilwadi Agada on colon cancer cell line COLO 205 using sulforhodamine B assay." Ayush Journal of Integrative Oncology 2, no. 2 (2025): 57–61. https://doi.org/10.4103/ajio.ajio_16_25.

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Abstract Context: Cancer is a group of diseases that occurs when abnormal cells grow uncontrollably or spread to other parts of the body. Despite the fact that contemporary science has developed powerful anticancer medications like Adriamycin, there are many negative side effects. Bilwadi Agada is an herbal drug possessing thirteen ingredients, among which many are proven to have anticancer activity; therefore, the goal of the current study is to use the Sulforhodamine B (SRB) assay to screen that Bilwadi Agada has anticancer potential on colon cancer cell line COLO 205. Materials and Methods:

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Singh, Vandana, Devika Srivastava, Prashant Pandey, et al. "Characterization, antibacterial and anticancer study of silk fibroin hydrogel." Journal of Drug Delivery and Therapeutics 13, no. 2 (2023): 21–31. http://dx.doi.org/10.22270/jddt.v13i2.5733.

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Purpose: Protein-based hydrogels such as silk fibroin hydrogel, are used in tissue engineering and regenerative medicine applications as they showed striking characteristics like biocompatibility and offered various benefits as biomaterials. The current study sought to prepare silk fibroin hydrogel and characterise it in order to assess its antibacterial and anticancer activity. Methodology: Silk fibroin hydrogel was prepared and characterized by using different microscopy methods, namely Scanning Electron Microscopy (SEM), Phase Contrast Electron (PCM) microscopy, and foldscope analysis

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Friedrich, Michael W. "Phylogenetic Analysis Reveals Multiple Lateral Transfers of Adenosine-5′-Phosphosulfate Reductase Genes among Sulfate-Reducing Microorganisms." Journal of Bacteriology 184, no. 1 (2002): 278–89. http://dx.doi.org/10.1128/jb.184.1.278-289.2002.

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ABSTRACT Lateral gene transfer affects the evolutionary path of key genes involved in ancient metabolic traits, such as sulfate respiration, even more than previously expected. In this study, the phylogeny of the adenosine-5′-phosphosulfate (APS) reductase was analyzed. APS reductase is a key enzyme in sulfate respiration present in all sulfate-respiring prokaryotes. A newly developed PCR assay was used to amplify and sequence a fragment (∼900 bp) of the APS reductase gene, apsA, from a taxonomically wide range of sulfate-reducing prokaryotes (n = 60). Comparative phylogenetic analysis of all

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Tung, Nguyen Thanh, Thi Pham Thi Diem, Dang Ngoc Sang, Do Thi Thao, and Hoang Tan Quang. "Biomass Accumulation of Gynostemma Pentaphyllum (Thunb.) Makino in Cell Suspension Cultures inhibiting Human Cancer Cell Growth." Research Journal of Biotechnology 17, no. 3 (2022): 61–68. http://dx.doi.org/10.25303/1703rjbt6168.

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Gynostemma pentaphyllum (Thunb.) Makino (GpM) is a medicinal plant in traditional medicine throughout Asia for the treatment of several diseases including cancer. GpM plant cell suspension cultures provide a time and cost effective well-controlled means promising a high-yielding biomass production of pharmaceutical compounds. The purpose of the current work is to investigate the effect of GpM cell suspension cultures on human cancer cell lines growth. The biomass was produced by cell suspension culture of GpM callus into 250 mL Erlenmeyer flask containing 50 mL of liquid medium culture. Gypeno

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Khojali, Weam M. A., Weiam Hussein, Mohammed Khaled Bin Break, et al. "Chemical Composition, Antibacterial Activity and In Vitro Anticancer Evaluation of Ochradenus baccatus Methanolic Extract." Medicina 59, no. 3 (2023): 546. http://dx.doi.org/10.3390/medicina59030546.

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Background and Objectives: Ochradenus baccatus belongs to the family Resedaceae. It is widely spread in Saudi Arabia and other countries in Southwest Asia. O. baccatus is extensively used in traditional medicine as an anti-inflammatory and antibacterial agent, in addition to being a vital source of food for certain desert animal species. The aim of the present study was to investigate the chemical composition and antibacterial/anticancer activities of O. baccatus methanolic extracts collected from Hail, Saudi Arabia. Materials and Methods: The O. baccatus extracts were obtained by macerating t

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Bobtaina, Eman, Enise Ece Gurdal, Irem Durmaz, Rengul Cetin Atalay, and Mine Yarim. "Synthesis and Cytotoxicity Studies on Novel Piperazinylacetamides." Letters in Drug Design & Discovery 16, no. 1 (2018): 45–51. http://dx.doi.org/10.2174/1570180815666180501124009.

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Background: In this study, nine novel compounds, bearing N-[2-(4-substituted piperazine-1-yl)acetyl]-N’-[bis-(4-fluorophenyl)methyl]piperazine structures were synthesized. Methods: Their cytotoxic properties were evaluated in vitro by NCI-60 Sulforhodamine B (SRB) assay against human cancer cell lines: Huh7 (hepatocellular), MCF7 (breast) and HCT116 (colorectal). Results and Conclusion: According to the activity data, most of the compounds are more cytotoxic than 5-fluorouracil against Huh7 and HCT116 cancer cell lines.

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Shawki, Mamdouh M., Alaa El Sadieque, Seham Elabd, and Maisa E. Moustafa. "Synergetic Effect of Tumor Treating Fields and Zinc Oxide Nanoparticles on Cell Apoptosis and Genotoxicity of Three Different Human Cancer Cell Lines." Molecules 27, no. 14 (2022): 4384. http://dx.doi.org/10.3390/molecules27144384.

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Cancer remains a leading cause of death worldwide, despite extraordinary progress. So, new cancer treatment modalities are needed. Tumor-treating fields (TTFs) use low-intensity, intermediate-frequency alternating electric fields with reported cancer anti-mitotic properties. Moreover, nanomedicine is a promising therapy option for cancer. Numerous cancer types have been treated with nanoparticles, but zinc oxide nanoparticles (ZnO NPs) exhibit biocompatibility. Here, we investigate the activity of TTFs, a sub-lethal dose of ZnO NPs, and their combination on hepatocellular carcinoma (HepG2), th

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Nguyen Thai, Hoang Tam, Thuy Vy Nguyen, and Thuy Duong Ho Huynh. "Sweet Wormwood and Tortoise Shell Decoction (Thanh Hao Miet Giap Thang) Induces DNA Damage, S-Phase Arrest, and Apoptosis in MCF-7 Cells via ATR-CHK1 Signaling Pathway." Evidence-Based Complementary and Alternative Medicine 2022 (March 5, 2022): 1–12. http://dx.doi.org/10.1155/2022/2358290.

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Introduction. Sweet wormwood and tortoise shell decoction, Thanh Hao Miet Giap Thang (THMGT) in Vietnamese, a traditional formula composed of five ingredients, is used in complementary care in Vietnam for patients who underwent conventional cancer treatment. To expand the clinical use and explore novel functions of THMGT, this study was conducted to investigate the effect of THMGT in terms of antiproliferative activity and selective cytotoxicity toward human breast cancer cells MCF-7. Methods. Cytotoxicity of THMGT against human breast cancer cells MCF-7 and primary fibroblasts from a heathy d

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Mohammed, Hiam Rifaat Hussien, Amr Helmy Moustafa El Bolok, Sherif Farouq Elgayar, and Maii Ibrahim Ali Sholqamy. "Evaluation the Effect of Natural Compounds: Vitamin C, Green Tea, and their Combination on Progression of Mg-63 Osteosarcoma Cell Line Cells. (An In Vitro Study)." Open Access Macedonian Journal of Medical Sciences 9, A (2021): 1277–84. http://dx.doi.org/10.3889/oamjms.2021.7894.

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BACKGROUND: Osteosarcoma (OS) is considered extremely rare type of bone tumor although it is the most common type of malignant bone tumor in children with less common occurrence in elderly patients. Herbal plants and phytoconstituents are recently used in the treatment of OS to avoid the side effects of chemotherapeutic drugs. AIM: The aims of the present study are to investigate the effect of natural compound Vitamin C, green tea, and their combination on OS cell line (Mg-63 cells) after 72 h. MATERIAL AND METHODS: Mg-63 cells were obtained from Nawah scientific and divided to four groups: Co

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Singh, Mukesh Kr, Gurdeep Singh, Ritesh Patel, Amrita Mishra, Arun Kr Mishra, and Sushil Kumar. "Evaluation of In vitro Antioxidant, Antimicrobial and Cytotoxicity Activities of Orthosiphon Pallidus Royle." International Journal of Pharmaceutical Sciences and Nanotechnology(IJPSN) 16, no. 3 (2023): 6497–503. http://dx.doi.org/10.37285/ijpsn.2023.16.3.3.

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Objective: The objective of present research work was to evaluate the in vitro antioxidant, antimicrobial and cytotoxicity activity of Orthosiphon pallidus Royle. Methods: Total phenolic and flavonoid content of hydroalcoholic extract (50:50) of Orthosiphon pallidus Royle hydroalcoholic extract (OPRHE) was estimated and the in vitro antioxidant activity was determined using ABTS radical scavenging assay and metal chelating assay. Antimicrobial activity was investigated by agar well diffusion method and MIC was determined. Cytotoxic assay was measured by the SRB method against breast canc

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Deplancke, Bart, Kai Finster, W. Vallen Graham, Chad T. Collier, Joel E. Thurmond, and H. Rex Gaskins. "Gastrointestinal and Microbial Responses to Sulfate-Supplemented Drinking Water in Mice." Experimental Biology and Medicine 228, no. 4 (2003): 424–33. http://dx.doi.org/10.1177/153537020322800413.

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There is increasing evidence that hydrogen sulfide (H2S), produced by intestinal sulfate-reducing bacteria (SRB), may be involved in the etiopathogenesis of chronic diseases such as ulcerative colitis and colorectal cancer. The activity of SRB, and thus H2S production, is likely determined by the availability of sulfur-containing compounds in the intestine. However, little is known about the impact of dietary or inorganic sulfate on intestinal sulfate and SRB-derived H2S concentrations. In this study, the effects of short-term (7 day) and long-term (1 year) inorganic sulfate supplementation of

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Shyaula, Sajan L., Teresa Shah, Rajitha Kalum Rathnayaka, et al. "Cytotoxicity of Compounds Isolated from Usnea aciculifera." Journal of Nepal Chemical Society 43, no. 2 (2023): 125–29. http://dx.doi.org/10.3126/jncs.v43i2.53357.

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Atranorin 1, Usnic acid 2, and D-Arabinitol 3 were isolated from Usnea aciculifera by extensive column chromatography. The structures were elucidated based on the comprehensive spectral analysis including FT IR, GC MS, ESI MS, 13C NMR, and 1H NMR. The compounds were evaluated for their cytotoxic activity against LN-229 glioblastoma cancer cell line by performing Sulforhodamine B assay (SRB). Usnic acid 2 showed strong cytotoxic activity against LN-229 glioblastoma cancer cell with an IC50 value of 3.09 μg/mL.

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Paula, Anabela, Mafalda Laranjo, Carlos Miguel Marto, et al. "Biodentine™ Boosts, WhiteProRoot®MTA Increases and Life® Suppresses Odontoblast Activity." Materials 12, no. 7 (2019): 1184. http://dx.doi.org/10.3390/ma12071184.

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(1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life®), mineral trioxide aggregate (WhiteProRoot®MTA) and calcium silicate (Biodentine™), in an odontoblast-like mouse cell line (MDPC-23). (2) Methods: Metabolic activity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT)assay, viability by the sulforhodamine B

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Mészáros, Beáta, Agota Csoti, Tibor G. Szanto, et al. "The hEag1 K+ Channel Inhibitor Astemizole Stimulates Ca2+ Deposition in SaOS-2 and MG-63 Osteosarcoma Cultures." International Journal of Molecular Sciences 23, no. 18 (2022): 10533. http://dx.doi.org/10.3390/ijms231810533.

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The hEag1 (Kv10.1) K+ channel is normally found in the brain, but it is ectopically expressed in tumor cells, including osteosarcoma. Based on the pivotal role of ion channels in osteogenesis, we tested whether pharmacological modulation of hEag1 may affect osteogenic differentiation of osteosarcoma cell lines. Using molecular biology (RT-PCR), electrophysiology (patch-clamp) and pharmacology (astemizole sensitivity, IC50 = 0.135 μM) we demonstrated that SaOS-2 osteosarcoma cells also express hEag1 channels. SaOS-2 cells also express to KCa1.1 K+ channels as shown by mRNA expression and paxill

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Le, Giau Hoang, Nha To Nguyen, Duong Thi Thuy Ngo, et al. "Biological test against breast adenocarcinoma cells (MCF-7) of acylated products of 3-methyl-4 thiorhodanine." Science and Technology Development Journal 18, no. 1 (2015): 63–69. http://dx.doi.org/10.32508/stdj.v18i1.1035.

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Rhodanine and its derivatives are not only valued for their interesting chemical properties but also present a wide range of bioactivities and their chemical and pharmacological applications have been investigated. From 3-methyl-4-thiorhodanine we synthesized S-acylated and C-acylated products using (some) carboxylic acid chlorides and the obtained products were studied the anti-proliferative activity by a SRB (sulforhodamine B) assay against a human breast cancer cell line. The results showed that C-acylated products are better inhibitors than S-acylated products.

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Xi, Meili, and Wenbin Tang. "Knockdown of Ezrin inhibited migration and invasion of cervical cancer cells in vitro." International Journal of Immunopathology and Pharmacology 34 (January 2020): 205873842093089. http://dx.doi.org/10.1177/2058738420930899.

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Cervical cancer is the fourth most common malignancy in women. The aim of this study was to investigate the functions of Ezrin in cervical cancer cells. Two cervical cancer cell lines, SiHa and CaSki, were cultured in vitro. Following the knockdown of Ezrin using siRNA, real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were applied to analyze Ezrin expression at the messenger RNA (mRNA) and protein levels. Subsequently, wound healing assay, transwell assay, and sulforhodamine B (SRB) assay were used to detect the migration, invasion, and viability of

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Subhash, Sneha, Amma C. R. Krishna Kumari, Vargheese V. Winston, and S. Sumathi. "Asterias rubens in High Dilutions exhibiting Anti-Cancer Activity in Breast Cancer Cell Line: An in vitro Study." Research Journal of Biotechnology 18, no. 11 (2023): 34–39. http://dx.doi.org/10.25303/1811rjbt034039.

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Asterias rubens constitutes saponins that represents a class of steroid oligoglycoside namely asterosaponins that has broad biological functions inclusive of anti-cancerous activity. The most common malignancy as well as the second largest cause of mortality related to cancer in women is breast carcinoma. The prime objective of this research is to assess the anti-cancer activity of Asterias rubens in its higher dilutions on MCF-7 cells and to evaluate the process of angiogenesis. In vitro cytotoxicity was assessed by MTT and SRB assay. To evaluate the long-term survival capacity and proliferat

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Reddy, Chada Raji, Muppidi Subbarao, Jonnalagadda Vijaykumar, et al. "One-Pot Synthesis of Triazolo-Heterolignans: Biological Evaluation and Molecular Docking Studies as Tubulin Inhibitors." Anti-Cancer Agents in Medicinal Chemistry 18, no. 12 (2019): 1702–10. http://dx.doi.org/10.2174/1871520618666180718104647.

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Background: The anti-mitotic activity of podophyllotoxin derivative targeting tubulin enzyme proved them as strong polymerization inhibitors. The introduction of heteroatom along with different heteroaryl systems in naturally obtained lignans created a latitude for design of bioactive components. A novel one-pot sequential propargylation/cycloaddition reaction strategy has been followed to synthesize triazolo-heterolignans. Objective: To screen anti-proliferative activity of novel heterolignans and to determine their mode of action. Method: SRB assay, Cytotoxicity evaluation, PI uptake for ana

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Al Malki, Jamila S., Nahed Ahmed Hussien, Lamia M. Akkad, Shatha O. Al Thurmani, and Anhal E. Al Motiri. "Green Synthesis of Silver and Titanium Oxide Nanoparticles Using Tea and Eggshell Wastes, Their Characterization, and Biocompatibility Evaluation." Sustainability 15, no. 15 (2023): 11858. http://dx.doi.org/10.3390/su151511858.

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Using biodegradable wastes represents a viable alternative to creating a sustainable economy that benefits all humans. The present study aimed to use daily used waste products, tea (TE) and eggshell (ES) wastes, to synthesize silver (AgNPs) and titanium oxide (TiO2NPs) nanoparticles, respectively. Firstly, the green-synthesized nanoparticles were characterized using an ultraviolet-visible spectrophotometer (UV-VIS), Scanning (SEM), transmission electron microscope (TEM), Dynamic light scattering (DLS), zeta potential analysis, X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spec

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Bulajić, Aleksandra, Ivana Djekić, Jelena Jović, Slobodan Krnjajić, Ana Vučurović, and Branka Krstić. "Incidence and Distribution of Iris yellow spot virus on Onion in Serbia." Plant Disease 93, no. 10 (2009): 976–82. http://dx.doi.org/10.1094/pdis-93-10-0976.

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In a survey to determine the presence and distribution of Iris yellow spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Bačka District)

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Heo, Chang-Su, Jong Soon Kang, Jeong-Wook Yang, et al. "Geliboluols A–D: Kaurane-Type Diterpenoids from the Marine-Derived Rare Actinomycete Actinomadura geliboluensis." Marine Drugs 23, no. 2 (2025): 78. https://doi.org/10.3390/md23020078.

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Four new kaurane-type diterpenoids, geliboluols A–D (1–4), along with one known analog (5), were isolated from the culture broth of the marine-derived rare actinomycete Actinomadura geliboluensis. The structures of compounds 1–4 were determined by spectroscopic analysis (HR-ESIMS, 1D, and 2D NMR), the MPA method, and by comparing their optical rotation values with those in the literature. The new compounds were evaluated for their cytotoxicity against seven blood cancer cell lines by a CellTiter-Glo (CTG) assay and six solid cancer cell lines by a sulforhodamine B (SRB) assay. Among the new co

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Manzano, Joe Anthony H., Elian Angelo Abellanosa, Jose Paolo Aguilar та ін. "Globospiramine from Voacanga globosa Exerts Robust Cytotoxic and Antiproliferative Activities on Cancer Cells by Inducing Caspase-Dependent Apoptosis in A549 Cells and Inhibiting MAPK14 (p38α): In Vitro and Computational Investigations". Cells 13, № 9 (2024): 772. http://dx.doi.org/10.3390/cells13090772.

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Bisindole alkaloids are a source of inspiration for the design and discovery of new-generation anticancer agents. In this study, we investigated the cytotoxic and antiproliferative activities of three spirobisindole alkaloids from the traditional anticancer Philippine medicinal plant Voacanga globosa, along with their mechanisms of action. Thus, the alkaloids globospiramine (1), deoxyvobtusine (2), and vobtusine lactone (3) showed in vitro cytotoxicity and antiproliferative activities against the tested cell lines (L929, KB3.1, A431, MCF-7, A549, PC-3, and SKOV-3) using MTT and CellTiter-Blue

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Samah DJEDDI, Κonstantinos DIMAS2, Wissam MAZOUZ, and Helen SKALTSA. "In vitro cytotoxicity of sesquiterpene lactones isolated from Centaurea pullata L. native to Algeria." Journal of Natural Product Research and Applications 1, no. 03 (2022): 68–74. http://dx.doi.org/10.46325/jnpra.v1i03.29.

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The phytochemical investigation of the aerial parts of Centaurea pullata L. from Algeria led to the isolation and identification of ten sesquiterpene lactones. The in vitro cytotoxic and antiproliferative potential of the isolated sesquiterpene lactones was tested against fives human cell lines (i.e., DLD1, SF268, MCF7, NCI-H460 and OVCAR3) using the sulforhodamine B (SRB) assay. In total ten compounds were tested, among which the elemanolide melitensin (5) proved the most active demonstrating a growth inhibitory activity below 100 μM against three cell lines NCI-H460, MCF7 and SF268, from the

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47

Murti, Y., S. Sharma, and P. Mishra. "IN VITRO CYTOTOXICITY OF CHROMATOGRAPHIC ELUTES OF CALOTROPIS PROCERA (AIT.) R BR. LEAVES AGAINST HUMAN HEPATOMA CELL LINE (HEPG2)." INDIAN DRUGS 53, no. 05 (2016): 48–52. http://dx.doi.org/10.53879/id.53.05.10461.

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Calotropis procera (Ait.) R. Br. leaves extract was prepared by maceration with using a ethanol as a solvent for seven days. The dried residue was dissolved in water and partitioned with n-butanol to obtained water and n-butanol fraction. The n-butanol fraction collected was subjected to column chromatography. Chloroform, chloroform:methanol (9:1), chloroform:methanol (7:1), chloroform:methanol (1:1) and methanol elutes of n-butanol fraction of ethanolic extract of Calotropis procera (Ait) R.Br. leaves were tested against human hepatoma cell line (HEPG2) by using SRB assay method of in vitro a

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Pedro-Hernández, Luis D., Ulises Organista-Mateos, Luis I. Allende-Alarcón, Elena Martínez-Klimova, Teresa Ramírez-Ápan, and Marcos Martínez-García. "Improvement of the Anticancer Activity of Chlorambucil and Ibuprofen via Calix[4]arene Conjugates." Medicinal Chemistry 16, no. 7 (2020): 984–90. http://dx.doi.org/10.2174/1573406415666190826162339.

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Background: One of the possible ways of improving the activity and selectivity profile of anticancer agents is to design drug carrier systems employing nanomolecules. Calix[4]arene derivatives and chlorambucil and ibuprofen are important compounds that exhibit interesting anticancer properties. Objective: The objective of this article is the synthesis of new calix[4]arene-derivative conjugates of chlorambucil or ibuprofen with potential anticancer activity. Methods: Cytotoxicity assays were determined using the protein-binding dye sulforhodamine B (SRB) in microculture to measure cell growth a

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Kour, Manmeet, Jagjeet Kour, Bisma Yousuf, and Shashank K. Singh. "Mechanistic Evaluation of Thymoquinone Derivative-Induced Apoptosis in Human Glioblastoma Cells." Journal of Pharmacy and Bioallied Sciences 17, Suppl 2 (2025): S1313—S1315. https://doi.org/10.4103/jpbs.jpbs_1967_24.

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ABSTRACT Background: Glioblastoma is a highly aggressive brain tumor with limited treatment options, necessitating the development of targeted and effective therapeutics. Thymoquinone (TQ), a natural compound with known anticancer potential, has been modified to enhance its efficacy. Materials and Methods: A series of TQ derivatives (SS-01 to SS-05) and the parent compound (SS-06) were synthesized and screened for cytotoxic activity using the Sulforhodamine B (SRB) assay against various cancer cell lines—glioblastoma (SNB-19, U251), breast carcinoma (MCF-7, MD-MBA-231), pancreatic carcinoma (M

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Helal, Mohamed A., Ahmed M. Abdel-Gawad, Omnia M. Kandil, et al. "Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro." Pathogens 9, no. 9 (2020): 740. http://dx.doi.org/10.3390/pathogens9090740.

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The anthelmintic effects of extracted coriander oil and five pure essential oil constituents (geraniol, geranyl acetate, eugenol, methyl iso-eugenol, and linalool) were tested, using larval motility assay, on the third-stage larvae (L3s) of Haemonchus contortus, Trichostrongylus axei, Teladorsagia circumcincta, Trichostrongylus colubriformis, Trichostrongylus vitrinus and Cooperia oncophora. Coriander oil and linalool, a major component of tested coriander oil, showed a strong inhibitory efficacy against all species, except C. oncophora with a half maximal inhibitory concentration (IC50) that

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