Relevant bibliographies by topics / Ss(ds-)-DNA

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters

Academic literature on the topic 'Ss(ds-)-DNA'

Author: Grafiati
Published: 11 March 2023
Last updated: 31 July 2025

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Journal articles on the topic "Ss(ds-)-DNA"

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Chandran, Harish, Nikhil Gopalkrishnan, Bernard Yurke, and John Reif. "Meta-DNA: synthetic biology via DNA nanostructures and hybridization reactions." Journal of The Royal Society Interface 9, no. 72 (2012): 1637–53. http://dx.doi.org/10.1098/rsif.2011.0819.

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Can a wide range of complex biochemical behaviour arise from repeated applications of a highly reduced class of interactions? In particular, can the range of DNA manipulations achieved by protein enzymes be simulated via simple DNA hybridization chemistry? In this work, we develop a biochemical system which we call meta-DNA (abbreviated as mDNA), based on strands of DNA as the only component molecules. Various enzymatic manipulations of these mDNA molecules are simulated via toehold-mediated DNA strand displacement reactions. We provide a formal model to describe the required properties and op
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2

Liu, H., M. I. Boulton, C. L. Thomas, D. A. M. Prior, K. J. Oparka, and J. W. Davies. "Maize Streak Virus Coat Protein Is Karyophyllic and Facilitates Nuclear Transport of Viral DNA." Molecular Plant-Microbe Interactions® 12, no. 10 (1999): 894–900. http://dx.doi.org/10.1094/mpmi.1999.12.10.894.

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Transport of maize streak virus (MSV) DNA into the nucleus of host cells is essential for virus replication and the presence of virus particles in the nuclei of infected cells implies that coat protein (CP) must enter the nucleus. To see if CP is imported into the nucleus in the absence of other viral gene products, the MSV CP gene was expressed in insect cells with a baculovirus vector system, and also in tobacco protoplasts with a cauliflower mosaic virus (CaMV) 35S promoter-driven transient gene expression vector. Immunofluorescent staining showed that the CP accumulated in the nuclei of bo
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3

De, Pallabi, Mandy M. Peak, and Karla K. Rodgers. "DNA Cleavage Activity of the V(D)J Recombination Protein RAG1 Is Autoregulated." Molecular and Cellular Biology 24, no. 15 (2004): 6850–60. http://dx.doi.org/10.1128/mcb.24.15.6850-6860.2004.

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ABSTRACT RAG1 and RAG2 catalyze the first DNA cleavage steps in V(D)J recombination. We demonstrate that the isolated central domain of RAG1 has inherent single-stranded (ss) DNA cleavage activity, which does not require, but is enhanced by, RAG2. The central domain, therefore, contains the active-site residues necessary to perform hydrolysis of the DNA phosphodiester backbone. Furthermore, the catalytic activity of this domain on ss DNA is abolished by addition of the C-terminal domain of RAG1. The inhibitory effects of this latter domain are suppressed on substrates containing double-strande
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4

Hauck, Bernd, Wei Zhao, Katherine High, and Weidong Xiao. "Intracellular Viral Processing, Not Single-Stranded DNA Accumulation, Is Crucial for Recombinant Adeno-Associated Virus Transduction." Journal of Virology 78, no. 24 (2004): 13678–86. http://dx.doi.org/10.1128/jvi.78.24.13678-13686.2004.

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ABSTRACT Adeno-associated virus (AAV) is a unique gene transfer vector which takes approximately 4 to 6 weeks to reach its expression plateau. The mechanism for this slow-rise expression profile was proposed to be inefficient second-strand DNA synthesis from the input single-stranded (ss) DNA viral genome. In order to clarify the status of ss AAV genomes, we generated AAV vectors labeled with bromodeoxyuridine (BrdU), a nucleotide analog that can be incorporated into the AAV genome and packaged into infectious virions. Since BrdU-DNA can be detected only by an anti-BrdU antibody when DNA is in
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5

Erben, Antonija, Josipa Matić, Nikola Basarić, and Ivo Piantanida. "The Phenanthridine-modified Tyrosine Dipeptide." Croatica chemica acta 92, no. 2 (2019): 249–58. http://dx.doi.org/10.5562/cca3542.

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Dipeptide 4 containing two unnatural amino acids, a modified tyrosine and a phenanthridine derivative, was synthesized. Binding of the dipeptide to a series of polynucleotides including ct-DNA, poly A - poly U, poly (dAdT)2, poly dG - poly dC and poly (dGdC)2 was investigated by thermal denaturation experiments, fluorescence spectroscopy and circular dichroism. Thermal denaturation experiments indicated that dipeptide 4 at pH 5.0, when phenanthridine is protonated, stabilizes ds-DNA, whereas it destabilizes ds-RNA. At pH 7.0, when the phenanthridine is not protonated, effects of 4 to the polyn
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6

Bastos, M., V. Castro, G. Mrevlishvili, and J. Teixeira. "Hydration of ds-DNA and ss-DNA by Neutron Quasielastic Scattering." Biophysical Journal 86, no. 6 (2004): 3822–27. http://dx.doi.org/10.1529/biophysj.104.039586.

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7

Clausen, J., and S. A. Nielsen. "The Use of In Vitro Cultured Lymphocytes for Tracing Mutagenic Activities of Chemicals." Alternatives to Laboratory Animals 14, no. 3 (1987): 168–71. http://dx.doi.org/10.1177/026119298701400314.

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Lymphocytes from normal, non-smoking human individuals not taking drugs were isolated from the peripheral blood by means of the lymphoprep method. The cells were cultured in RPMI medium with 10% fetal calf serum and stimulated with Phytohemagglutinin. A mutagen such as 3-methylcholanthrene was added for varying periods of time. Then the subspecies of DNA, i.e. double and single stranded DNA (ds-DNA and ss-DNA), were separated by the alkaline elution technique and quantitated by fluorimetric estimation. The mutagen induced a significant rise in the level of ss-DNA, but no changes in ds-DNA coul
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8

Amundsen, S. K., A. M. Neiman, S. M. Thibodeaux, and G. R. Smith. "Genetic dissection of the biochemical activities of RecBCD enzyme." Genetics 126, no. 1 (1990): 25–40. http://dx.doi.org/10.1093/genetics/126.1.25.

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Abstract RecBCD enzyme of Escherichia coli is required for the major pathway of homologous recombination following conjugation. The enzyme has an ATP-dependent DNA unwinding activity, ATP-dependent single-stranded (ss) and double-stranded (ds) DNA exonuclease activities, and an activity that makes a ss DNA endonucleolytic cut near Chi sites. We have isolated and characterized ten mutations that reduced recombination proficiency and inactivated some, but not all, activities of RecBCD enzyme. One class of mutants had weak ds DNA exonuclease activity and lacked Chi-dependent DNA cleavage activity
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9

Kirisawa, Rikio, Rika Kato, Koichi Furusaki, and Takashi Onodera. "Universal Virucidal Activity of Calcium Bicarbonate Mesoscopic Crystals That Provides an Effective and Biosafe Disinfectant." Microorganisms 10, no. 2 (2022): 262. http://dx.doi.org/10.3390/microorganisms10020262.

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We investigated the virucidal effects in solution of a new type of disinfectant, calcium bicarbonate mesoscopic crystals, designated CAC-717, against various types of virus. CAC-717 in solution is alkaline (pH 12.4) and has a self-electromotive force that generates pulsed electrical fields. Upon application to human skin, the pH of the solution becomes 8.4. CAC-717 contains no harmful chemicals and is thus non-irritating and harmless to humans and animals. Its virucidal effects were tested against six types of animal virus: enveloped double-strand (ds)-DNA viruses, non-enveloped ds-DNA viruses
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10

Chaudhury, AM, ES Dennis, and RIS Brettell. "Gene-Expression Following T-DNA Transfer Into Plant Cells Is Aphidicolin-Sensitive." Functional Plant Biology 21, no. 2 (1994): 125. http://dx.doi.org/10.1071/pp9940125.

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A transient assay for gene-expression was used to study the early events of T-DNA transfer. Particularly, it was asked if gene expression following T-DNA transfer required DNA replication in the host cell. A β-glucuronidase gene, linked to a CaMV 35S promoter (35S-GUS, engineered so that it was inactive in Agrobacterium tumefaciens) was introduced into Nicotiana plumbaginifolia protoplasts via a disarmed supervirulent strain of Agrobacterium tumefaciens. High β-glucuronidase activity appeared after 3 days of co-cultivation. The activity required the presence of the vir functions of agrobacteri
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Dissertations / Theses on the topic "Ss(ds-)-DNA"

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Nkoua, Ngavouka Maryse Dadina. "Conformational properties of variable density DNA nanobrushes." Doctoral thesis, Università degli studi di Trieste, 2015. http://hdl.handle.net/10077/11129.

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2013/2014<br>Advanced nanotechnologies allow the manipulation of molecules with nanoscale precision, and can be used for the production of sensitive devices for protein or nucleic acids detection for clinical use. DNA nano-assemblies are an excellent route for ultrasensitive DNA/RNA detection and for DNA-protein conjugated immobilization, for bio interaction studies, through the careful detection of single strand DNA (ssDNA) hybridization with complementary target sequences. For DNA nanoscale devices, the control of DNA surface density and conformation is crucial in order to achieve the hi
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Book chapters on the topic "Ss(ds-)-DNA"

1

Carter, Paul. "Mutagenesis facilitated by the removal or introduction of unique restriction sites." In Directed Mutagenesis. Oxford University PressOxford, 1991. http://dx.doi.org/10.1093/oso/9780199631414.003.0001.

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Abstract Over the last decade, a variety of efficient and reliable methods have been developed for the construction of site-directed mutations in DNA using synthetic oligonucleotides (reviewed in refs 1-3). This has revolutionized the study of gene regulation and protein -structure and function. All of these mutagenesis methods fall into one of three broad strategies. One approach uses an oligonucleotide complimentary to part of a single-stranded (ss) DNA template but containing an internal mismatch(es) to direct the desired mutation (see Section 1.1, and Chapters 2 to 5 and 9). A second strat
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Journal articles
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