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Relevant bibliographies by topics / SSCP [Single Strand Conformation Polymorphism ]

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Academic literature on the topic 'SSCP [Single Strand Conformation Polymorphism ]'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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Journal articles on the topic "SSCP [Single Strand Conformation Polymorphism ]"

1

Maréchal, V. "SSCP (single strand conformation polymorphism)." EMC - Biologie médicale 3, no. 1 (2008): 1–5. http://dx.doi.org/10.1016/s2211-9698(08)71401-8.

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AL-ADHAMI, BATOL H., FLORENCE HUBY-CHILTON, BURTON W. BLAIS, AMALIA MARTINEZ-PEREZ, NEIL B. CHILTON, and ALVIN A. GAJADHAR. "Rapid Discrimination of Salmonella Isolates by Single-Strand Conformation Polymorphism Analysis." Journal of Food Protection 71, no. 10 (2008): 1960–66. http://dx.doi.org/10.4315/0362-028x-71.10.1960.

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A molecular typing technique was developed for the differentiation of Salmonella isolates based on single-strand conformation polymorphism (SSCP) analysis of amplicons generated by PCR. Amplicons from parts of the fimA (both the 5′ and 3′ ends), mdh, invA, and atpD genes were generated separately from a panel of Salmonella strains representing Salmonella bongori, and four subspecies and 17 serovars of Salmonella enterica. These amplicons were subjected to SSCP analysis for differentiation of the salmonellae on the basis of different conformational forms arising due to nucleotide sequence variations in the target genes. Several distinct SSCP banding patterns (a maximum of 14 each for atpD and fimA 3′ end) were observed with this panel of Salmonella strains for amplicons generated from each target gene. The best discrimination of Salmonella subspecies and serovar was achieved from the SSCP analysis of a combination of at least three gene targets: atpD, invA, and either mdh or fimA 3′ end. This demonstrates the applicability of SSCP analysis as an important additional method to classical typing approaches for the differentiation of foodborne Salmonella isolates. SSCP is simple to perform and should be readily transferable to food microbiology laboratories with basic PCR capability.

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Satoh, T., M. Kobayashi, M. Kaneda, M. Tanihiro, K. Okada, and K. Ueda. "Genotypical classification of neutrophil Fc gamma receptor III by polymerase chain reaction-single-strand conformation polymorphism." Blood 83, no. 11 (1994): 3312–15. http://dx.doi.org/10.1182/blood.v83.11.3312.3312.

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Abstract We classified the genotype of neutrophil Fc gamma receptor III (FcRIII) (CD16) with a new method. Genomic DNA from mononuclear cells of 39 unrelated healthy donors (13 NA1/NA1, 13 NA2/NA2, and 13 NA1/NA2 typed serologically) were subjected to polymerase chain reaction (PCR) to amplify the polymorphic third exon of the FcRIII genes. The PCR products were heat denatured, electrophoresed, and visualized by silver staining. Allelic differences were detected by distinctive electrophoretic patterns of each single strand, depending on their sequence specific conformations (single-strand conformation polymorphism [SSCP]). The genotypes of neutrophil FcRIII determined by this method were consistent with the phenotypes of NA antigens determined by serologic examinations. These results indicate that the PCR-SSCP system is a very useful tool for genotyping of the neutrophil FcRIII.

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Satoh, T., M. Kobayashi, M. Kaneda, M. Tanihiro, K. Okada, and K. Ueda. "Genotypical classification of neutrophil Fc gamma receptor III by polymerase chain reaction-single-strand conformation polymorphism." Blood 83, no. 11 (1994): 3312–15. http://dx.doi.org/10.1182/blood.v83.11.3312.bloodjournal83113312.

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We classified the genotype of neutrophil Fc gamma receptor III (FcRIII) (CD16) with a new method. Genomic DNA from mononuclear cells of 39 unrelated healthy donors (13 NA1/NA1, 13 NA2/NA2, and 13 NA1/NA2 typed serologically) were subjected to polymerase chain reaction (PCR) to amplify the polymorphic third exon of the FcRIII genes. The PCR products were heat denatured, electrophoresed, and visualized by silver staining. Allelic differences were detected by distinctive electrophoretic patterns of each single strand, depending on their sequence specific conformations (single-strand conformation polymorphism [SSCP]). The genotypes of neutrophil FcRIII determined by this method were consistent with the phenotypes of NA antigens determined by serologic examinations. These results indicate that the PCR-SSCP system is a very useful tool for genotyping of the neutrophil FcRIII.

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Iizuka, M., K. Hayashi, and T. Sekiya. "Single-strand conformation polymorphism (SSCP) at the D8S86 locus." Nucleic Acids Research 19, no. 22 (1991): 6346. http://dx.doi.org/10.1093/nar/19.22.6346.

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Lizuka, M., K. Hayashi, and T. Sekiya. "Single-strand conformation polymorphism (SSCP) at the D8S86 locus." Nucleic Acids Research 20, no. 2 (1992): 388. http://dx.doi.org/10.1093/nar/20.2.388.

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MUGGIA, Lucia, and Martin GRUBE. "Fungal composition of lichen thalli assessed by single strand conformation polymorphism." Lichenologist 42, no. 4 (2010): 461–73. http://dx.doi.org/10.1017/s0024282909990752.

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AbstractFungi that are unrelated to the mycobiont species frequently colonize lichens. Some of these fungal colonists are described lichenicolous fungi, lichen parasites and pathogens that produce recognizable morphological characters, while others apparently produce no noticeable structures. Here we apply the single strand conformation polymorphism (SSCP) technique to directly assess the abundance of different fungi in lichens. Twenty-eight lichen thalli were chosen, some with and some without externally visible symptoms of parasite infection, and these were subjected to total DNA extraction. PCR was conducted with fungal-specific primers for the ITS region of ribosomal DNA. Single strands of the products were separated on native acrylamide gels. The majority of lichen specimens, both infected and those without symptoms, displayed more than one band in the stained gels. In one case, 14 bands were detected using SSCP. Some of these bands apparently represent other neighbouring lichens in the habitat, but many are apparently non-lichen-forming. Since few lichen-associated fungi have been cultured and sequenced, it is difficult to know if SSCP bands represent obligate lichenicolous fungi, other asymptomatic lichen parasites, or fungi not obligately associated with lichens, but our results indicate that large numbers of non-lichen-forming fungi commonly co-occur with lichens in nature. For specimens of the filamentous lichens Cystocoleus ebeneus and Racodium rupestre we used cloned sequences to compare the number of sequences obtained by the SSCP method to the number obtained by direct sequencing of thallus extracts, and we generally found that more sequences could be detected by SSCP than could be seen by direct sequencing.

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Markoff, Arseni, Alex Savov, Vladimir Vladimirov, Nadia Bogdanova, Ivo Kremensky, and Varban Ganev. "Optimization of single-strand conformation polymorphism analysis in the presence of polyethylene glycol." Clinical Chemistry 43, no. 1 (1997): 30–33. http://dx.doi.org/10.1093/clinchem/43.1.30.

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Abstract We report optimization of single-strand conformation polymorphism (SSCP) analysis in the presence of polyethylene glycol. The protocol developed separates single-strand conformers in a much shorter time (1–3 h) than conventional SSCP protocols and broadens the applicability of SSCP analysis from 150 to as much as 500 bp of DNA by different percentages of GC content present. We conclude that addition of polyethylene glycol helps improve the differential separation of conformers and, in combination with high-resolution polyacrylamide gel electrophoresis, offers an alternative to previous SSCP analysis protocols. This protocol should be very useful for clinical applications in routine detection of mutations as well as for research purposes.

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Isam, Zahraa, Rabab Omran Al-jelawi-, and Ammad Hassan Mahmood. "DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE-STRAND CONFORMATION POLYMORPHISM-POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE." Asian Journal of Pharmaceutical and Clinical Research 11, no. 1 (2018): 205. http://dx.doi.org/10.22159/ajpcr.2017.v11i1.22063.

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Objective: The uromodulin, a glycoprotein, expressed and secreted by epithelial kidney cells lining the thick ascending limb of the Henle’s loop. It is encoded by the UMOD gene in humans. Our objective was to analyze single nucleotide polymorphisms (SNPs) in the UMOD promoter region in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD).Methods: The blood samples were collected from 100 patients with CKD (50) and ESRD (50), who admitted at Merjan Teaching Hospital in Babylon Province, Iraq (February-July 2016). In addition, 50 blood samples of healthy control. The SNPs of UMOD promoter region was investigated using single-strand conformation polymorphism-polymerase chain polymorphisms (SSCP-PCR) and DNA sequencing techniques.Results: UOMD promoter region polymorphisms using PCR-SSCP and sequencing DNA appeared three different conformational haplotypes, including A\G 49 haplotype (5 bands), A\G 49 and C\A 247 haplotype (5 bands), and C\G 45 and A\G 49 haplotype (6 bands) distributed among CKD and ESRD cases, due to the presence of three SNPs. There was no association between band numbers of PCR-SSCP with ESRD and CKD compared with a control group.Conclusion: SSCP-PCR is a good screening method to detect genetic variations in an uromodulin promoter region.

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Isam, Zahraa, Rabab Omran Al-jelawi-, and Ammad Hassan Mahmood. "DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE-STRAND CONFORMATION POLYMORPHISM-POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE." Asian Journal of Pharmaceutical and Clinical Research 11, no. 1 (2018): 205. http://dx.doi.org/10.22159/ajpcr.2018.v11i1.22063.

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Objective: The uromodulin, a glycoprotein, expressed and secreted by epithelial kidney cells lining the thick ascending limb of the Henle’s loop. It is encoded by the UMOD gene in humans. Our objective was to analyze single nucleotide polymorphisms (SNPs) in the UMOD promoter region in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD).Methods: The blood samples were collected from 100 patients with CKD (50) and ESRD (50), who admitted at Merjan Teaching Hospital in Babylon Province, Iraq (February-July 2016). In addition, 50 blood samples of healthy control. The SNPs of UMOD promoter region was investigated using single-strand conformation polymorphism-polymerase chain polymorphisms (SSCP-PCR) and DNA sequencing techniques.Results: UOMD promoter region polymorphisms using PCR-SSCP and sequencing DNA appeared three different conformational haplotypes, including A\G 49 haplotype (5 bands), A\G 49 and C\A 247 haplotype (5 bands), and C\G 45 and A\G 49 haplotype (6 bands) distributed among CKD and ESRD cases, due to the presence of three SNPs. There was no association between band numbers of PCR-SSCP with ESRD and CKD compared with a control group.Conclusion: SSCP-PCR is a good screening method to detect genetic variations in an uromodulin promoter region.

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Dissertations / Theses on the topic "SSCP [Single Strand Conformation Polymorphism ]"

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Genini, Sem. "Establishment of quick-methods to reveal DNA-polymorphisms single strand conformational polymorphism (SSCP) and heteroduplex analysis (HA) /." Zurich : Swiss Federal Institute of Technology, Department of Animal Sciences, Breeding Biology, 2002. http://e-collection.ethbib.ethz.ch/show?type=dipl&nr=50.

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Jorge, Simone Bordignon de. "Otimização da tecnica de SSCP (Single Strand Conformation Polymorphism) para triagem de mutações nos genes da globina [alfa] humana." [s.n.], 2002. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310884.

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Orientadores : Maria de Fatima Sonati, Monica Barbosa de Melo<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas<br>Made available in DSpace on 2018-08-03T14:43:34Z (GMT). No. of bitstreams: 1 Jorge_SimoneBordignonde_M.pdf: 1672707 bytes, checksum: 1ca1b039159c022f6091792558490b00 (MD5) Previous issue date: 2002<br>Resumo: Mutações de ponto e pequenas inserções ou deleções nos genes da globina a humana podem produzir variantes estruturais de cadeia a e a-talassemia. As mutações podem ser identificadas tanto pelo sequenciamento total dos genes, como por métodos de triagem, os quais selecionam o exon mutado para, então, ser seqüenciado. Embora sejam pequenos (cerca de 1Kb, com 3 exons e 2 introns), os genes da globina a são duplicados (a2 and a1) e extremamente ricos em ligações G-C, o que torna difícil a desnaturação e reduz a eficiência do sequenciamento, causando freqüentes artefatos e necessidade de repetições. Como o sequenciamento é ainda um método demorado e de custo elevado para muitos laboratórios, nós modificamos algumas condições do Single Strand Conformation Polymorphism (SSCP) com o intuito de otimizar a triagem de mutações nos genes da globina a humana<br>Abstract: Point mutations and small insertions or deletions in the human a-globin genes may produce a-chain structural variants and a-thalassemia. Mutations can be detected either by direct sequencing of the whole gene or by screening methods, which select the mutated exon for sequencing. Although small (around 1 Kb, 3 exons and 2 introns), the a-globin genes are duplicate (a2 and a1) and extremely G-C rich, what makes them difficult to denature and reduces sequencing efficiency, causing frequent artifacts and the need of repetitions. As DNA sequencing is still a time-consuming and expensive method for many laboratories, we modified some conditions of the Single Strand Conformation Polymorphism (SSCP) in order to optimize mutation detection in the a-globin genes<br>Mestrado<br>Ciencias Biomedicas<br>Mestre em Ciências Médicas

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Contador, Luciana. "Etude de la structure chimique et microbiologique de l'interface air-mer en baie de Guanabara (Rio de Janeiro, Brésil)." Paris 6, 2006. http://www.theses.fr/2006PA066158.

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La microcouche de surface (SML) correspond au premier millimètre de la colonne d’eau. Située à l’interface air-eau, la SML est un environnement unique, riche en microorganismes, en matières organiques, en hydrocarbures et présentant des conditions extrêmes par l’impact des radiations UV et les fortes concentrations de polluants. Les échantillons ont été prélevés dans la SML et dans l’eau sous-jacente (UW) à 7 stations de la Baie de Guanabara, Brésil. Cette étude a permis de caractériser l’abondance et la diversité des bactéries et des hydrocarbures présents dans la SML de la baie. L’origine des hydrocarbures a été analysée par des marqueurs chimiques par chromatographie en phase gazeuse. Sur le plan microbiologique, 43 souches bactériennes ont été isolées et identifiées. Leur résistance aux radiations UV a été évaluée à l’aide d’un simulateur solaire. La SML de la baie est bien définie par rapport à l’UW et enrichie en hydrocarbures et en bactéries. Le bactérioneuston est plus diversifié que le bactérioplancton. La plupart des souches testées sont très résistantes aux UV et sont des espèces potentiellement intéressantes sur le plan biotechnologique.

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Hedley, Paula. "The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations." Master's thesis, University of Cape Town, 2003. http://hdl.handle.net/11427/4271.

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Includes bibliographical references.<br>This thesis reports the development of Single-Strand Conformation Polymorphism (SSCP) technique to assess sequence level variation within the Major Histocompatibility Complex (MHC) DRB1 gene in four South African buffalo populations. MHC gene products are involved in the immune response, and so variation within these genes provides information on the immunological fitness of the population under study. The aims of this study were: (i) to develop the SSCP technique; (ii) to investigate the level of genetic variation at the peptide binding region (PBR) of the DRB1 gene in four South African buffalo populations. (iii) This data was then compared to data generated previously in a study on the same populations using microsatellite DNA, (iv) the statistical comparisons were used to assess the appropriateness of SSCP data for population genetic analysis. Levels of heterozygosity, allelic diversity and population differentiation were quantified using MHC DRB1 gene. The amplified region (Exon 2 of the DRB1 gene) showed high levels of variability, with 77 alleles found in the 84 individuals examined using SSCP analyses.

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Peyruchaud, Olivier. "Analyse des polymorphismes du complexe glycoprotéique GPIIb-IIIa plaquettaire par PCR-SSCP : application à la Thrombasthénie de Glanzmann et aux systèmes alloantigéniques." Bordeaux 2, 1995. http://www.theses.fr/1995BOR28356.

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Contador, Luciana. "Étude de la structure chimique et microbiologique de l'interface air-mer en Baie de Guanabara (Rio de Janeiro, Brésil)." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2006. http://tel.archives-ouvertes.fr/tel-00122547.

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La microcouche de surface (SML) correspond au premier millimètre de la colonne d'eau. Située à l'interface air-eau, la SML est un environnement unique, riche en microorganismes, en matières organiques, en hydrocarbures et présentant des conditions extrêmes par l'impact des radiations UV et les fortes concentrations de polluants. Les échantillons ont été prélevés dans la SML et dans l'eau sous-jacente (UW) à 7 stations de la Baie de Guanabara, Brésil. Cette étude a permis de caractériser l'abondance et la diversité des bactéries et des hydrocarbures présents dans la SML de la baie. L'origine des hydrocarbures a été analysée par des marqueurs chimiques par chromatographie en phase gazeuse. Sur le plan microbiologique, 43 souches bactériennes ont été isolées et identifiées. Leur résistance aux radiations UV a été évaluée à l'aide d'un simulateur solaire. La SML de la baie est bien définie par rapport à l'UW et enrichie en hydrocarbures et en bactéries. Le bactérioneuston est plus diversifié que le bactérioplancton. La plupart des souches testées sont très résistantes aux UV et sont des espèces potentiellement intéressantes sur le plan biotechnologique.

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Charinthon, Ngamamonpirat Jitra Waikagul. "Sequence variation of Gnathostoma spinigerum mitochondrial DNA by single-strand conformation polymorphism analysis /." Abstract, 2003. http://mulinet3.li.mahidol.ac.th/thesis/2546/46E-Charinthon-N.pdf.

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King, Stephanie. "Capillary Electrophoresis Single-Strand Conformation Polymorphism Analysis for Monitoring Bacteria during the Remediation of TNT-Contaminated Soil." Ohio University / OhioLINK, 2004. http://www.ohiolink.edu/etd/view.cgi?ohiou1108061640.

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ZIHA, ZARIFI ISABELLE. "Mutations de la p53 dans les cancers du sein : optimisation des conditions de single strand conformation polymorphism." Besançon, 1993. http://www.theses.fr/1993BESA3105.

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Duthoit, Frédérique. "Dynamique des communautés microbiennes du fromage d'A. O. C. Salers par analyse Single-Strand Conformation Polymorphism : lien avec les caractéristiques sensorielles des fromages." Clermont-Ferrand 2, 2003. http://www.theses.fr/2003CLF21453.

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Le but de ce travail est de relier les dynamiques microbiennes de présence et d'activité suivies par analyse Single Strand Conformation Polymorphism au cours de la fabrication du fromage d'AOC Salers aux caractéristiques sensorielles du fromage affiné. Des régions des ADNr et ARNr 16S extraits des fromages ont été amplifiées par PCR et RT-PCR et analysées par SSCP. Les profils microbiens ont été reliés aux données sensorielles par régression Partial Least Square. Des séquences correspondant à des bactéries lactiques, entérobactéries, bactéries Gram positif à bas et haut GC% ont été identifiées. La comparaison des profils issus des ARN et des ADN montre des dynamiques bactériennes et des niveaux d'activité différents. Les corrélations avec les variables sensorielles sont complexes. Chaque descripteur est expliqué par de nombreux groupes microbiens. Cela montre l'intérêt de prendre en compte les dynamiques bactériennes de présence et d'activité lors de la fabrication des fromages

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Book chapters on the topic "SSCP [Single Strand Conformation Polymorphism ]"

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Leung, Kim Hung, and Shea Ping Yip. "Single Strand Conformation Polymorphism (SSCP) Analysis." In Springer Protocols Handbooks. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_9.

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Jordan, William C., Katherine Foley, and Michael W. Bruford. "Single-Strand Conformation Polymorphism (SSCP) Analysis." In Molecular Tools for Screening Biodiversity. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-009-0019-6_30.

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Qian, Feng, and Gregory G. Germino. "Single-Strand Conformation Polymorphism (SSCP) Analysis." In Techniques in Molecular Medicine. Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_5.

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Vorechovsky, Igor. "Single-Strand Conformation Polymorphism (SSCP) Analysis." In Medical Biomethods Handbook. Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-870-6:073.

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Schmalenberger, Achim, and Christoph C. Tebbe. "Profiling the Diversity of Microbial Communities with Single-Strand Conformation Polymorphism (SSCP)." In Methods in Molecular Biology. Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-712-9_6.

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Offermans, M. T. C., H. Meyer, and N. D. Zegers. "Identification of Pathogens Using Single/Double Strand Conformation Polymorphism (SSCP/DSCP) Analysis." In Rapid Methods for Analysis of Biological Materials in the Environment. Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-015-9534-6_20.

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Lin, Shu-Wha, Shu-Rung Lin, and Ming-Ching Shen. "Hemophilia A: Characterization of Genetic Defects by the Single Strand Conformation Polymorphism (SSCP)." In Current Aspects of Blood Coagulation, Fibrinolysis, and Platelets. Springer Japan, 1993. http://dx.doi.org/10.1007/978-4-431-68323-0_9.

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Zehbe, Ingeborg, Eva Rylander, Jan Sällström, and Erik Wilander. "Genotyping of Human Papillomavirus (HPV) by Single-Strand Conformational Polymorphism (SSCP)." In Immunology of Human Papillomaviruses. Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2449-6_10.

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Dohrmann, Anja B., and Christoph C. Tebbe. "Section 3 update: Microbial community analysis by PCR-single-strand conformation polymorphism (PCR-SSCP)." In Molecular Microbial Ecology Manual. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_316.

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Jacques Godon, Jean. "Capillary Electrophoresis- Single Strand Conformation Polymorphism (CE-SSCP) as a Tool for Water Traceability." In Food Traceability and Authenticity. CRC Press, 2017. http://dx.doi.org/10.1201/9781351228435-8.

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Conference papers on the topic "SSCP [Single Strand Conformation Polymorphism ]"

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Zhao, Yan, Jianzheng Li, Jie Zhang, Dong Li, and Shuang Zhao. "Dynamics of Methangens During Steady-State of Anaerobic Baffled Reactor Using Single Strand Conformation Polymorphism." In 2009 Asia-Pacific Power and Energy Engineering Conference. IEEE, 2009. http://dx.doi.org/10.1109/appeec.2009.4918306.

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Ono, Koichi, Mitsuyasu Koike, Takuhito Ohse, Atsuko Takagi, and Yasuyuki Ikeda. "High-throughput Single-strand Conformation Polymorphism Analysis of an LPL Gene Mutation by Temperature-controlled On-chip Capillary Electrophoresis." In 2006 International Conference on Microtechnologies in Medicine and Biology. IEEE, 2006. http://dx.doi.org/10.1109/mmb.2006.251527.

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