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Journal articles on the topic 'ST53'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Giampetruzzi, Annalisa, Maria Saponari, Giuliana Loconsole, et al. "Genome-Wide Analysis Provides Evidence on the Genetic Relatedness of the Emergent Xylella fastidiosa Genotype in Italy to Isolates from Central America." Phytopathology® 107, no. 7 (2017): 816–27. http://dx.doi.org/10.1094/phyto-12-16-0420-r.

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Xylella fastidiosa is a plant-pathogenic bacterium recently introduced in Europe that is causing decline in olive trees in the South of Italy. Genetic studies have consistently shown that the bacterial genotype recovered from infected olive trees belongs to the sequence type ST53 within subspecies pauca. This genotype, ST53, has also been reported to occur in Costa Rica. The ancestry of ST53 was recently clarified, showing it contains alleles that are monophyletic with those of subsp. pauca in South America. To more robustly determine the phylogenetic placement of ST53 within X. fastidiosa, we

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Bive, Bive Zono, Rosalie Sacheli, Hippolyte Situakibanza Nani-Tuma, et al. "Clinical epidemiology and high genetic diversity amongst Cryptococcus spp. isolates infecting people living with HIV in Kinshasa, Democratic Republic of Congo." PLOS ONE 17, no. 5 (2022): e0267842. http://dx.doi.org/10.1371/journal.pone.0267842.

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Neuromeningeal cryptococcosis (NMC) is a life-threatening opportunistic infection in advanced HIV disease patients (AHDP). It is caused by Cryptococcus spp. complexes and mainly occurs in sub-Saharan Africa. In this study, we performed molecular characterization and antifungal susceptibility profiling of Cryptococcus isolates from AHDP in Kinshasa (DRC). Additionally, we investigated a possible association between NMC severity factors and the Cryptococcus neoformans (Cn) multilocus sequence typing (MLST) profiles. We characterized the isolates using PCR serotyping, MALDI-TOF MS, internal trans

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3

Fierro, Annalisa, Antonella Liccardo, and Francesco Porcelli. "A lattice model to manage the vector and the infection of the Xylella fastidiosa on olive trees." Scientific Reports 9, no. 1 (2019): 8723. https://doi.org/10.1038/s41598-019-44997-4.

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<strong>Since October 2013 a new devastating plant disease, known as Olive Quick Decline Syndrome, has been killing most of the olive trees distributed in Apulia, South Italy. </strong><em>Xylella fastidiosa pauca </em><strong>ST53 is the plant pathogenic bacterium responsible for the disease, and the adult Meadow Spittlebug, </strong><em>Philaenus spumarius </em><strong>(L.) (Hemiptera Aphrophoridae), is its main vector. This study proposes a lattice model for the pathogen invasion of olive orchard aimed at identifying an appropriate strategy for arresting the infection, built on the manageme

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Hidad, Soemeja, Boas van der Putten, Robin van Houdt, Caroline Schneeberger, and Sacha Daniëlle Kuil. "Recurrent E. coli Urinary Tract Infections in Nursing Homes: Insight in Sequence Types and Antibiotic Resistance Patterns." Antibiotics 11, no. 11 (2022): 1638. http://dx.doi.org/10.3390/antibiotics11111638.

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Introduction: Recurrent urinary tract infections (rUTI) largely contribute to antibiotic use in older adults. Understanding the genetic characteristics of Escherichia coli (E.coli) is needed to identify patients at risk for recurrence. The aim of this study was to obtain a greater understanding of the genetics of E. coli rUTI in nursing home residents. Methods: This is a secondary analysis of a multicenter Dutch nursing home study (PROGRESS). E. coli strains from residents with a suspected UTI and positive urine culture were analyzed using antimicrobial susceptibility testing and whole-genome

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Picciotti, Ugo, Nada Lahbib, Valdete Sefa, Francesco Porcelli, and Francesca Garganese. "Aphrophoridae Role in Xylella fastidiosa subsp. pauca ST53 Invasion in Southern Italy." Pathogens 10, no. 8 (2021): 1035. http://dx.doi.org/10.3390/pathogens10081035.

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The Philaenus spumarius L. (Hemiptera Aphrophoridae) is a xylem-sap feeder vector that acquires Xylella fastidiosa subsp. pauca ST53 during feeding on infected plants. The bacterium is the plant pathogen responsible for olive quick decline syndrome that has decimated olive trees in Southern Italy. Damage originates mainly from the insect vector attitude that multiplies the pathogen potentialities propagating Xf in time and space. The principal action to manage insect-borne pathogens and to contain the disease spread consists in vector and transmission control. The analysis of an innovative and

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MORALEJO, Eduardo, Bàrbara QUETGLAS, Marina MONTESINOS, et al. "Outbreak of Xylella fastidiosa subsp. pauca ST53 affecting wild and cultivated olive trees on the island of Mallorca, Spain." Phytopathologia Mediterranea 63, no. 3 (2024): 475–80. https://doi.org/10.36253/phyto-15891.

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The Balearic Islands have emerged as a hotspot for the invasive plant pathogen Xylella fastidiosa (Xf). Since 2016, the Xf subsp. fastidiosa and multiplex have been detected causing almond leaf scorch and Pierce’s disease on the island of Mallorca, Spain, and a new sequence type (ST), ST80, of subsp. pauca is infecting wild and cultivated olive trees on the island of Ibiza. In addition, Xf subsp. multiplex ST81 is widespread in scrublands, and causes mild, sub-lethal dieback of wild olive trees in Menorca and Mallorca. A new outbreak is here reported of the Xf subsp. pauca in the municipality

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Slukin, P. V., E. I. Astashkin, E. M. Aslanyan, et al. "Characterization of virulent Escherichia coli strains isolated from patients with urological infection." Journal of microbiology, epidemiology and immunobiology 98, no. 6 (2022): 671–84. http://dx.doi.org/10.36233/0372-9311-134.

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Objective. Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) affect 150 million people annually.Purpose: Characteristics of non-hospital strains of UPEC isolated from patients with UTI in Yaroslavl in 2016– 2017.Materials and methods. Susceptibility of UPEC strains (n = 20) to antibacterials was measured by the serial dilution method; the antibiotic resistance and virulence genes, phylogroups, O-serogroups and sequence types were identified by PCR and whole genome sequencing. The virulence of the strains was studied using the model of Galleria mellonella larvae.Re

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FANG, Y., P. ZHU, Q. LI, et al. "Multilocus sequence typing of 102 Burkholderia pseudomallei strains isolated from China." Epidemiology and Infection 144, no. 9 (2016): 1917–23. http://dx.doi.org/10.1017/s0950268815003052.

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SUMMARYThe phylogenetic and epidemiological relationships of 102 Burkholderia pseudomallei clinical isolates from different geographical and population sources in China were investigated by multilocus sequence typing (MLST). The MLST data were analysed using the e-BURST algorithm, and an unweighted pair-group method with arithmetic mean dendrogram was constructed based on the pair-wise differences in the allelic profiles of the strains. Forty-one sequence types (STs) were identified, of which eight were novel (ST1341, ST1345, ST1346, ST1347, ST1348, ST1349, ST1350, ST1351). No geographical-spe

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Abuyusuf, Md, Arif Robin, Ji-Hee Lee, et al. "Glucosinolate Profiling and Expression Analysis of Glucosinolate Biosynthesis Genes Differentiate White Mold Resistant and Susceptible Cabbage Lines." International Journal of Molecular Sciences 19, no. 12 (2018): 4037. http://dx.doi.org/10.3390/ijms19124037.

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Sclerotinia stem rot (white mold), caused by the fungus Sclerotinia sclerotiorum, is a serious disease of Brassica crops worldwide. Despite considerable progress in investigating plant defense mechanisms against this pathogen, which have revealed the involvement of glucosinolates, the host–pathogen interaction between cabbage (Brassica oleracea) and S. sclerotiorum has not been fully explored. Here, we investigated glucosinolate profiles and the expression of glucosinolate biosynthesis genes in white-mold-resistant (R) and -susceptible (S) lines of cabbage after infection with S. sclerotiorum.

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Rizzo, Domenico, Matteo Bracalini, Sara Campigli, et al. "Quantitative Real-Time PCR Based on SYBR Green Technology for the Identification of Philaenus italosignus Drosopoulos & Remane (Hemiptera Aphrophoridae)." Plants 11, no. 23 (2022): 3314. http://dx.doi.org/10.3390/plants11233314.

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The use of molecular tools to identify insect pests is a critical issue, especially when rapid and reliable tests are required. We proposed a protocol based on qPCR with SYBR Green technology to identify Philaenus italosignus (Hemiptera, Aphrophoridae). The species is one of the three spittlebugs able to transmit Xylella fastidiosa subsp. pauca ST53 in Italy, together with Philaenus spumarius and Neophilaenus campestris. Although less common than the other two species, its identification is key to verifying which role it can play when locally abundant. The proposed assay shows analytical speci

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Lahbib, Nada, Ugo Picciotti, Valdete Sefa, Sonia Boukhris-Bouhachem, Francesco Porcelli, and Francesca Garganese. "Zelus renardii Roaming in Southern Italy." Insects 13, no. 2 (2022): 158. http://dx.doi.org/10.3390/insects13020158.

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This study collects data from the literature and updates our Zelus renardii Kolenati, 1856 (Leafhopper Assassin Bug, LAB) prey knowledge. The literature consists of ca. 170 entries encompassing the years 1856 to 2021. This reduviid originated in the Nearctic region, but has entered and acclimatised in many Mediterranean countries. Our quantitative predation experiments—in the laboratory on caged plants plus field or environmental observations—confirm that LAB prefers a selected array of prey. Laboratory predation tests on living targets (Hemiptera, Coleoptera, Diptera, and Hymenoptera) agree w

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Stavrum, R., M. Mphahlele, K. Ovreas, et al. "High Diversity of Mycobacterium tuberculosis Genotypes in South Africa and Preponderance of Mixed Infections among ST53 Isolates." Journal of Clinical Microbiology 47, no. 6 (2009): 1848–56. http://dx.doi.org/10.1128/jcm.02167-08.

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13

Ambrico, Paolo Francesco, Stefania Zicca, Marianna Ambrico, et al. "Low Temperature Plasma Strategies for Xylella fastidiosa Inactivation." Applied Sciences 12, no. 9 (2022): 4711. http://dx.doi.org/10.3390/app12094711.

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The quarantine bacterium Xylella fastidiosa was first detected in Salento (Apulia, Italy) in 2013 and caused severe symptoms in olives, leading to plant death. The disease, named Olive Quick Decline Syndrome (OQDS), is caused by the strain “De Donno” ST53 of the subspecies pauca of this bacterium (XfDD), which is spread by the insect Philaenus spumarius. The epidemic poses a serious threat to the agricultural economy and the landscape, as X. fastidiosa infects several plant species and there is yet no recognized solution. Research on OQDS is focused on finding strategies to control its spread

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Elhussein, Ahmed, Biagia Musio, Stefano Todisco, et al. "Non-Targeted Spectranomics for the Early Detection of Xylella fastidiosa Infection in Asymptomatic Olive Trees, cv. Cellina di Nardò." Molecules 28, no. 22 (2023): 7512. http://dx.doi.org/10.3390/molecules28227512.

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Olive quick decline syndrome (OQDS) is a disease that has been seriously affecting olive trees in southern Italy since around 2009. During the disease, caused by Xylella fastidiosa subsp. pauca sequence type ST53 (Xf), the flow of water and nutrients within the trees is significantly compromised. Initially, infected trees may not show any symptoms, making early detection challenging. In this study, young artificially infected plants of the susceptible cultivar Cellina di Nardò were grown in a controlled environment and co-inoculated with additional xylem-inhabiting fungi. Asymptomatic leaves o

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Sanz, Juan Carlos, Iciar Rodríguez-Avial, Esther Ríos, Luis García-Comas, María Ordobás, and Emilia Cercenado. "Increase of serotype 8, ST53 clone, as the prevalent strain of Streptococcus pneumoniae causing invasive disease in Madrid, Spain (2012–2015)." Enfermedades Infecciosas y Microbiología Clínica 38, no. 3 (2020): 105–10. http://dx.doi.org/10.1016/j.eimc.2019.05.006.

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Sanz, Juan Carlos, Iciar Rodríguez-Avial, Esther Ríos, Luis García-Comas, María Ordobás, and Emilia Cercenado. "Increase of serotype 8, ST53 clone, as the prevalent strain of Streptococcus pneumoniae causing invasive disease in Madrid, Spain (2012–2015)." Enfermedades infecciosas y microbiologia clinica (English ed.) 38, no. 3 (2020): 105–10. http://dx.doi.org/10.1016/j.eimce.2019.05.008.

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Joseph Paul Marius Koualiagnigni, Jean Paul Assam Assam, Moussa Moupe, et al. "Evaluation of polymorphism of Mycobacterium tuberculosis complex and its association with age group and HIV sero-status in Ngaoundéré-Cameroon." International Journal of Science and Technology Research Archive 4, no. 2 (2023): 040–46. http://dx.doi.org/10.53771/ijstra.2023.4.2.0057.

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Pulmonary tuberculosis is a millennial scourge that is still current, and considered one of the most communicable diseases in the world. This study aimed to assess the genetic diversity of isolates of the Mycobacterium tuberculosis complex (MTBC) from pulmonary tuberculosis patients in Ngaoundéré. We estimates it from newly diagnosed sputum smear-positive patients by spoligotyping method. In total we obtained 21 different profiles including 9 orphan profiles and 12 clusters grouping from 2 to 54 strains. The comparison of the profiles of the strains of Ngaoundéré studied here with those of the

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Pecora, Nicole, Stacy Holzbauer, Xiong Wang, et al. "Genomic analysis of Clostridioides difficile in two regions reveals a diversity of strains and limited transmission." Infection Control & Hospital Epidemiology 41, S1 (2020): s237—s238. http://dx.doi.org/10.1017/ice.2020.793.

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Background: The epidemic NAP1/027 Clostridioides difficile strain (MLST1, ST1) that emerged in the mid-2000 is on the decline. The current distribution of C. difficile strain types and their transmission dynamics are poorly defined. We performed whole-genome sequencing (WGS) of C. difficile isolates in 2 regions to identify the predominant multilocus sequence types (MLSTs) in community- and healthcare-associated cases and potential transmission between cases using whole-genome single-nucleotide polymorphism (SNP) analysis. Methods: Isolates were collected through the CDC Emerging Infections Pr

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Ibrahim, Khaled M., Abdlrhman M. Alsonosi, Mahmoud B. Agena, Bassam A. Elgamoudi, and Stephen J. Forsythe. "Multiplex Determination of K-Antigen and Colanic Acid Capsule Variants of Cronobacter sakazakii." Genes 15, no. 10 (2024): 1282. http://dx.doi.org/10.3390/genes15101282.

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Cronobacter sakazakii is associated with the ingestion of contaminated reconstituted powdered infant formula (PIF), resulting in necrotizing enterocolitis, sepsis and meningitis in neonatal infants. Potential virulence determinants include the variable capsular polysaccharides; K-antigen and colanic acid (CA). Strains encoding for the capsule variant K2:CA2 have been strongly associated with neonatal meningitis cases. This study aimed to develop and apply a multiplex PCR assay to determine C. sakazakii K-antigen and colanic acid types. Twenty-six strains of C. sakazakii which had previously be

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Corbu, Viorica Maria, Andreea Ștefania Dumbravă, Irina Gheorghe-Barbu, and Ortansa Csutak. "Epiphytic Yeasts from South Romania for Preventing Food Microbial Contamination." Life 14, no. 9 (2024): 1087. http://dx.doi.org/10.3390/life14091087.

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Epiphytic yeasts represent an important source for the development of novel strategies aiming to combat food microbial contamination. The present study deals with the characterization of nine yeast strains belonging to Starmerella, Candida, Metschinikowia, Lachancea, Kodamaea and Pichia genera, isolated from the surface of plants from the Botanical Garden “Dimitrie Brandza” (Bucharest, Romania) for use as antimicrobial and probiotic agents. The tests involved the determination of the safe status, cell growth under stress conditions, and activity against pathogenic Candida and bacteria strains,

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Scala, Valeria, Marco Scortichini, Federico Marini, et al. "Assessment of Fatty Acid and Oxylipin Profile of Resprouting Olive Trees Positive to Xylella fastidiosa subsp. pauca in Salento (Apulia, Italy)." Plants 13, no. 16 (2024): 2186. http://dx.doi.org/10.3390/plants13162186.

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Xylella fastidiosa subsp. pauca ST53 (XFP), the causal agent of olive quick decline syndrome (OQDS), was thoroughly investigated after a 2013 outbreak in the Salento region of Southern Italy. Some trees from Ogliarola Salentina and Cellina di Nardò, susceptible cultivars in the Gallipoli area, the first XFP infection hotspot in Italy, have resprouted crowns and are starting to flower and yield fruits. Satellite imagery and Normalized Difference Vegetation Index analyses revealed a significant improvement in vegetation health and productivity from 2018 to 2022 of these trees. Lipid molecules ha

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Massella, Elisa, Cameron J. Reid, Max L. Cummins, et al. "Snapshot Study of Whole Genome Sequences of Escherichia coli from Healthy Companion Animals, Livestock, Wildlife, Humans and Food in Italy." Antibiotics 9, no. 11 (2020): 782. http://dx.doi.org/10.3390/antibiotics9110782.

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Animals, humans and food are all interconnected sources of antimicrobial resistance (AMR), allowing extensive and rapid exchange of AMR bacteria and genes. Whole genome sequencing (WGS) was used to characterize 279 Escherichia coli isolates obtained from animals (livestock, companion animals, wildlife), food and humans in Italy. E. coli predominantly belonged to commensal phylogroups B1 (46.6%) and A (29%) using the original Clermont criteria. One hundred and thirty-six sequence types (STs) were observed, including different pandemic (ST69, ST95, ST131) and emerging (ST10, ST23, ST58, ST117, S

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Gião, Joana, Célia Leão, Teresa Albuquerque, Lurdes Clemente, and Ana Amaro. "Antimicrobial Susceptibility of Enterococcus Isolates from Cattle and Pigs in Portugal: Linezolid Resistance Genes optrA and poxtA." Antibiotics 11, no. 5 (2022): 615. http://dx.doi.org/10.3390/antibiotics11050615.

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Enterococci are part of the commensal gut microbiota of mammals, with Enterococcus faecalis and Enterococcus faecium being the most clinically relevant species. This study assesses the prevalence and diversity of enterococcal species in cattle (n = 201) and pig (n = 249) cecal samples collected in 2017. Antimicrobial susceptibility profiles of E. faecium (n = 48) and E. faecalis (n = 84) were assessed by agar and microdilution methods. Resistance genes were screened through PCR and nine strains were analyzed by Whole Genome Sequencing. A wide range of enterococci species was found colonizing t

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Pot, Matthieu, Yann Reynaud, David Couvin, et al. "Emergence of a Novel Lineage and Wide Spread of a blaCTX-M-15/IncHI2/ST1 Plasmid among Nosocomial Enterobacter in Guadeloupe." Antibiotics 11, no. 10 (2022): 1443. http://dx.doi.org/10.3390/antibiotics11101443.

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Between April 2018 and August 2019, a total of 135 strains of Enterobacter cloacae complex (ECC) were randomly collected at the University Hospital Center of Guadeloupe to investigate the structure and diversity of the local bacterial population. These nosocomial isolates were initially identified genetically by the hsp60 typing method, which revealed the clinical relevance of E. xiangfangensis (n = 69). Overall, 57/94 of the third cephalosporin-resistant strains were characterized as extended-spectrum-β-lactamase (ESBL) producers, and their whole-genome was sequenced using Illumina technology

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Mokaddas, Eiman, Mohammad Asadzadeh, Shabeera Syed, and M. John Albert. "High Prevalence of Novel Sequence Types in Streptococcus pneumoniae That Caused Invasive Diseases in Kuwait in 2018." Microorganisms 12, no. 1 (2024): 225. http://dx.doi.org/10.3390/microorganisms12010225.

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Background: Multilocus sequence typing (MLST) is used to gain insight into the population genetics of bacteria in the form of sequence type (ST). MLST has been used to study the evolution and spread of virulent clones of Streptococcus pneumoniae in many parts of the world. Such data for S. pneumoniae are lacking for the countries of the Arabian Peninsula, including Kuwait. Methods: We determined the STs of all 31 strains of S. pneumoniae from invasive diseases received at a reference laboratory from various health centers in Kuwait during 2018 by MLST. The relationship among the isolates was d

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Villalón, Pilar, Marta Bárcena, María José Medina-Pascual, et al. "National Surveillance of Tetracycline, Erythromycin, and Clindamycin Resistance in Invasive Streptococcus pyogenes: A Retrospective Study of the Situation in Spain, 2007–2020." Antibiotics 12, no. 1 (2023): 99. http://dx.doi.org/10.3390/antibiotics12010099.

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Background: This work reports on antimicrobial resistance data for invasive Streptococcus pyogenes in Spain, collected by the ‘Surveillance Program for Invasive Group A Streptococcus’, in 2007–2020. Methods: emm typing was determined by sequencing. Susceptibility to penicillin, tetracycline, erythromycin, and clindamycin was determined via the E-test. tetM, tetO, msrD, mefA, ermB, ermTR, and ermT were sought by PCR. Macrolide-resistant phenotypes (M, cMLSB, and iMLSB) were detected using the erythromycin–clindamycin double-disk test. Resistant clones were identified via their emm type, multilo

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Wareth, Gamal, Christian Brandt, Lisa D. Sprague, Heinrich Neubauer, and Mathias W. Pletz. "Spatio-Temporal Distribution of Acinetobacter baumannii in Germany—A Comprehensive Systematic Review of Studies on Resistance Development in Humans (2000–2018)." Microorganisms 8, no. 3 (2020): 375. http://dx.doi.org/10.3390/microorganisms8030375.

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Acinetobacter (A.) baumannii has gained global notoriety as a significant nosocomial pathogen because it is frequently associated with multi-drug resistance and hospital-based outbreaks. There is a substantial difference in the incidence of A. baumannii infections between different countries and within Germany. However, its continuous spread within Germany is a matter of concern. A systematic literature search and analysis of the literature published between 2000 and 2018 on A. baumannii in humans was performed. Forty-four studies out of 216 articles met the criteria for inclusion, and were se

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MARCADE, G., S. BRISSE, S. BIALEK, et al. "The emergence of multidrug-resistantKlebsiella pneumoniaeof international clones ST13, ST16, ST35, ST48 and ST101 in a teaching hospital in the Paris region." Epidemiology and Infection 141, no. 8 (2012): 1705–12. http://dx.doi.org/10.1017/s0950268812002099.

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SUMMARYDespite infection control measures, an important increase in the extended-spectrum β-lactamase (ESBL)-producingKlebsiella pneumoniaeincidence density occurred in our hospital from 2006 onwards. This study, focusing on the 2005–2007 period, was performed in an attempt to explain this increase. ESBLs were characterized, isolates were typed by ERIC2-PCR, and sequence type (ST) of clustered isolates was determined. Temporal-spatial relationships of patients were analysed to assess possible cross-contamination. Of the 74 ESBL-producing isolates, 30 (40%) were detected at admission, 53 (71·5%

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Piccirilli, Alessandra, Sabrina Cherubini, Anna Maria Azzini, et al. "Whole-Genome Sequencing (WGS) of Carbapenem-Resistant K. pneumoniae Isolated in Long-Term Care Facilities in the Northern Italian Region." Microorganisms 9, no. 9 (2021): 1985. http://dx.doi.org/10.3390/microorganisms9091985.

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K. pneumoniae (KPN) is one of the widest spread bacteria in which combined resistance to several antimicrobial groups is frequent. The most common β-lactamases found in K. pneumoniae are class A carbapenemases, both chromosomal-encoded (i.e., NMCA, IMI-1) and plasmid-encoded (i.e., GES-enzymes, IMI-2), VIM, IMP, NDM, OXA-48, and extended-spectrum β-lactamases (ESBLs) such as CTX-M enzymes. In the present study, a total of 68 carbapenem-resistant KPN were collected from twelve long-term care facilities (LTCFs) in the Northern Italian region. The whole-genome sequencing (WGS) of each KPN strain

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Dione, Michel M., Stanny Geerts, and Martin Antonio. "Characterisation of novel strains of multiply antibiotic-resistant Salmonella recovered from poultry in Southern Senegal." Journal of Infection in Developing Countries 6, no. 05 (2011): 436–42. http://dx.doi.org/10.3855/jidc.1530.

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Introduction: Non-typhoidal Salmonella (NTS) contamination in poultry and poultry products is a major cause of food-borne disease in humans. This study presents the molecular epidemiology of NTS isolated from poultry in Senegal. Methodology: A total of 261 NTS recovered from broiler farms, chicken carcasses and street vendors were characterized using random amplification of polymorphic DNA (RAPD) and multilocus sequence typing (MLST) techniques. Results: We observed 20 distinct RAPD profiles corresponding to 18 different serotypes. Strains from each of these 20 groups were further analysed usi

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Alegría, Ángel, Marta Arias-Temprano, Isabel Fernández-Natal, Jose M. Rodríguez-Calleja, María-Luisa García-López, and Jesús A. Santos. "Molecular Diversity of ESBL-Producing Escherichia coli from Foods of Animal Origin and Human Patients." International Journal of Environmental Research and Public Health 17, no. 4 (2020): 1312. http://dx.doi.org/10.3390/ijerph17041312.

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Dissemination of enterobacteria that produce extended spectrum β-lactamases (ESBL) throughout the food chain has become an important health concern. This work aimed to evaluate the occurrence of ESBL-producing bacteria in foods of animal origin and to investigate the similarities between food and human isolates. The presence of beta-lactam-resistant Enterobacteriaceae was analyzed in 108 food samples, isolating 10 strains of Escherichia coli, one strain of Citrobacter freundi, and one of Hafnia alvei. E. coli isolates were compared to a group of 15 strains isolated from human patients by antib

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GERHOLD, G., M. H. SCHULZE, U. GROSS, and W. BOHNE. "Multilocus sequence typing and CTX-M characterization of ESBL-producing E. coli: a prospective single-centre study in Lower Saxony, Germany." Epidemiology and Infection 144, no. 15 (2016): 3300–3304. http://dx.doi.org/10.1017/s0950268816001412.

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SUMMARYThe increasing prevalence of extended-spectrum β-lactamase (ESBL)-producing Gram-negative bacteria is a serious threat for current healthcare settings. In this study we investigated the molecular epidemiology of ESBL-producing E. coli at the University Medical Center Göttingen in Lower Saxony, Germany. All E. coli isolates with an ESBL phenotype were collected during a 6-month period in 2014. Multilocus sequence typing and CTX-M characterization were performed on 160 isolates. Of the ESBL-producing isolates 95·6% were CTX-M positive. Compared to recent Germany-wide studies, we found CTX

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Ricci, Maria Luisa, Silvia Fillo, Andrea Ciammaruconi, et al. "Genome analysis of Legionella pneumophila ST23 from various countries reveals highly similar strains." Life Science Alliance 5, no. 6 (2022): e202101117. http://dx.doi.org/10.26508/lsa.202101117.

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Legionella pneumophila serogroup 1 (Lp1) sequence type (ST) 23 is one of the most commonly detected STs in Italy where it currently causes all investigated outbreaks. ST23 has caused both epidemic and sporadic cases between 1995 and 2018 and was analysed at genomic level and compared with ST23 isolated in other countries to determine possible similarities and differences. A core genome multi-locus sequence typing (cgMLST), based on a previously described set of 1,521 core genes, and single-nucleotide polymorphisms (SNPs) approaches were applied to an ST23 collection including genomes from Ital

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Massella, Elisa, Federica Giacometti, Paolo Bonilauri, et al. "Antimicrobial Resistance Profile and ExPEC Virulence Potential in Commensal Escherichia coli of Multiple Sources." Antibiotics 10, no. 4 (2021): 351. http://dx.doi.org/10.3390/antibiotics10040351.

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We recently described the genetic antimicrobial resistance and virulence profile of a collection of 279 commensal E. coli of food-producing animal (FPA), pet, wildlife and human origin. Phenotypic antimicrobial resistance (AMR) and the role of commensal E. coli as reservoir of extra-intestinal pathogenic Escherichia coli (ExPEC) virulence-associated genes (VAGs) or as potential ExPEC pathogens were evaluated. The most common phenotypic resistance was to tetracycline (76/279, 27.24%), sulfamethoxazole/trimethoprim (73/279, 26.16%), streptomycin and sulfisoxazole (71/279, 25.45% both) among the

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Vingino, Alexandria, Marilyn C. Roberts, Michelle Wainstein, et al. "Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem." Antibiotics 10, no. 10 (2021): 1201. http://dx.doi.org/10.3390/antibiotics10101201.

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E. coli was isolated from the Salish Sea (Puget Sound) ecosystem, including samples of marine and fresh water, and wildlife dependent on this environment. E. coli isolates were assessed for phenotypic and genotypic resistance to antibiotics. A total of 305 E. coli isolates was characterized from samples collected from: marine water obtained in four quadrants of the Salish Sea; select locations near beaches; fresh water from streams near marine beaches; and fecal samples from harbor porpoises (Phocoena phocoena), harbor seals (Phoca vitulina), river otters (Lontra canadensis), and English sole

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Flament-Simon, Saskia-Camille, Marie-Hélène Nicolas-Chanoine, Vanesa García, et al. "Clonal Structure, Virulence Factor-encoding Genes and Antibiotic Resistance of Escherichia coli, Causing Urinary Tract Infections and Other Extraintestinal Infections in Humans in Spain and France during 2016." Antibiotics 9, no. 4 (2020): 161. http://dx.doi.org/10.3390/antibiotics9040161.

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Escherichia coli is the main pathogen responsible for extraintestinal infections. A total of 196 clinical E. coli consecutively isolated during 2016 in Spain (100 from Lucus Augusti hospital in Lugo) and France (96 from Beaujon hospital in Clichy) were characterized. Phylogroups, clonotypes, sequence types (STs), O:H serotypes, virulence factor (VF)-encoding genes and antibiotic resistance were determined. Approximately 10% of the infections were caused by ST131 isolates in both hospitals and approximately 60% of these infections were caused by isolates belonging to only 10 STs (ST10, ST12, ST

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Silva-Costa, C., A. Friães, M. Ramirez, and J. Melo-Cristino. "Differences between Macrolide-Resistant and -Susceptible Streptococcus pyogenes: Importance of Clonal Properties in Addition to Antibiotic Consumption." Antimicrobial Agents and Chemotherapy 56, no. 11 (2012): 5661–66. http://dx.doi.org/10.1128/aac.01133-12.

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ABSTRACTA steady decline in macrolide resistance amongStreptococcus pyogenes(group A streptococci [GAS]) in Portugal was reported during 1999 to 2006. This was accompanied by alterations in the prevalence of macrolide resistance phenotypes and in the clonal composition of the population. In order to test whether changes in the macrolide-resistant population reflected the same changing patterns of the overall population, we characterized both macrolide-susceptible and -resistant GAS associated with a diagnosis of tonsillo-pharyngitis recovered in the period from 2000 to 2005 in Portugal. Pulsed

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Gostev, Vladimir V., O. S. Sulian, P. A. Pavlova, et al. "Genomic characterization of oxacillin-susceptible mecA-positive Staphylococcus aureus ST59." Clinical Microbiology and Antimicrobial Chemotherapy 25, no. 2 (2023): 116–22. http://dx.doi.org/10.36488/cmac.2023.2.116-122.

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Objective. To characterize the genomes of oxacillin-susceptible mecA-positive Staphylococcus aureus ST59 isolated in St. Petersburg. Materials and Methods. Nine oxacillin-susceptible mecA-positive of S. aureus isolates (OS-MRSA) of ST59 were included in the study. The isolates were obtained from children who showed no clinical signs of staphylococcal infections during nasal screening of S. aureus in St. Petersburg in 2018–2019. One isolate was obtained from an adult patient with skin and soft tissue infection (SSTI). The susceptibility to antibiotics and whole genome sequencing were performed.

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Abreu-Salinas, Fátima, Dafne Díaz-Jiménez, Isidro García-Meniño, et al. "High Prevalence and Diversity of Cephalosporin-Resistant Enterobacteriaceae Including Extraintestinal Pathogenic E. coli CC648 Lineage in Rural and Urban Dogs in Northwest Spain." Antibiotics 9, no. 8 (2020): 468. http://dx.doi.org/10.3390/antibiotics9080468.

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The aim of this work was to assess the prevalence of extended spectrum-β-lactamase (ESBL)- and carbapenemase-producing Enterobacteriaceae in fecal samples recovered from rural and urban healthy dogs in Northwest Spain (Galicia) to identify potential high-risk clones and to molecularly characterize positive isolates regarding the genes coding for ESBL/pAmpC resistance and virulence. Thirty-five (19.6%) out of 179 dogs were positive for cephalosporin-resistant Enterobacteriaceae, including Escherichiacoli and Klebsiella pneumoniae (39 and three isolates, respectively). All the isolates were mult

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Nunney, Leonard, Danel B. Vickerman, Robin E. Bromley, et al. "Recent Evolutionary Radiation and Host Plant Specialization in the Xylella fastidiosa Subspecies Native to the United States." Applied and Environmental Microbiology 79, no. 7 (2013): 2189–200. http://dx.doi.org/10.1128/aem.03208-12.

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ABSTRACTThe bacterial pathogen,Xylella fastidiosa, infects many plant species in the Americas, making it a good model for investigating the genetics of host adaptation. We used multilocus sequence typing (MLST) to identify isolates of the native U.S. subsp.multiplexthat were largely unaffected by intersubspecific homologous recombination (IHR) and to investigate how their evolutionary history influences plant host specialization. We identified 110 “non-IHR” isolates, 2 minimally recombinant “intermediate” ones (including the subspecific type), and 31 with extensive IHR. The non-IHR and interme

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Sundqvist, Martin, Susanne Granholm, Umaer Naseer, et al. "Within-Population Distribution of Trimethoprim Resistance in Escherichia coli before and after a Community-Wide Intervention on Trimethoprim Use." Antimicrobial Agents and Chemotherapy 58, no. 12 (2014): 7492–500. http://dx.doi.org/10.1128/aac.03228-14.

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ABSTRACTA 2-year prospective intervention on the prescription of trimethoprim reduced the use by 85% in a health care region with 178,000 inhabitants. Here, we performed before-and-after analyses of the within-population distribution of trimethoprim resistance inEscherichia coli. Phylogenetic and population genetic methods were applied to multilocus sequence typing data of 548 consecutively collectedE. coliisolates from clinical urinary specimens. Results were analyzed in relation to antibiotic susceptibility and the presence and genomic location of different trimethoprim resistance gene class

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Taniwan, Steven, Dwi Suryanto, and Isnaini Nurwahyuni. "ISOLASI DAN KARAKTERISASI PARSIAL BAKTERI PELARUT FOSFAT DARI GUANO GUA KAMPRET DAN UJI KEMAMPUANNYA DALAM MENINGKATKAN PERTUMBUHAN TANAMAN." JURNAL BIOSAINS 2, no. 2 (2016): 82. http://dx.doi.org/10.24114/jbio.v2i2.4219.

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Bakteri pelarut fosfat merupakan bakteri yang mampu melarutkan fosfat yang tidak tersedia menjadi tersedia sehingga dapat diserap oleh tanaman. Studi tentang isolasi dan karakterisasi parsial bakteri pelarut fosfat dari guano gua kampret dan uji kemampuannya dalam meningkatkan pertumbuhan tanaman telah dilakukan. Dua isolat bakteri pelarut fosfat berhasil diisolasi dan keduanya berasal dari kelompok bakteri Gram positif, yaitu ST02 dan ST03. Isolat bakteri diuji pada benih tanaman cabai merah selama 30 hari. Rata-rata pertambahan tinggi tanaman tertinggi diperoleh dari perlakuan bakteri ST03 s

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Dziva, Francis, Heidi Hauser, Thomas R. Connor, et al. "Sequencing and Functional Annotation of Avian Pathogenic Escherichia coli Serogroup O78 Strains Reveal the Evolution of E. coli Lineages Pathogenic for Poultry via Distinct Mechanisms." Infection and Immunity 81, no. 3 (2012): 838–49. http://dx.doi.org/10.1128/iai.00585-12.

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ABSTRACTAvian pathogenicEscherichia coli(APEC) causes respiratory and systemic disease in poultry. Sequencing of a multilocus sequence type 95 (ST95) serogroup O1 strain previously indicated that APEC resemblesE. colicausing extraintestinal human diseases. We sequenced the genomes of two strains of another dominant APEC lineage (ST23 serogroup O78 strains χ7122 and IMT2125) and compared them to each other and to the reannotated APEC O1 sequence. For comparison, we also sequenced a human enterotoxigenicE. coli(ETEC) strain of the same ST23 serogroup O78 lineage. Phylogenetic analysis indicated

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Takano, Tomomi, Wataru Higuchi, Taketo Otsuka, et al. "Novel Characteristics of Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains Belonging to Multilocus Sequence Type 59 in Taiwan." Antimicrobial Agents and Chemotherapy 52, no. 3 (2007): 837–45. http://dx.doi.org/10.1128/aac.01001-07.

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ABSTRACTCommunity-acquired methicillin-resistantStaphylococcus aureus(CA-MRSA) strains, which often produce Panton-Valentine leucocidin (PVL), are increasingly noted worldwide. In this study, we examined 42 MRSA strains (25 PVL-positive [PVL+] strains and 17 PVL-negative [PVL−] strains) isolated in Taiwan for their molecular characteristics. The PVL+MRSA strains included CA-MRSA strains with multilocus sequence type (ST) 59 (major PVL+MRSA in Taiwan), its variants, and worldwide CA-MRSA ST30 strains. The PVL−MRSA strains included the pandemic Hungarian MRSA ST239 strain, the Hungarian MRSA ST2

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Kitahara, Maki, Mitsuo Sakamoto, Sayaka Tsuchida, et al. "Bacteroides stercorirosoris sp. nov. and Bacteroides faecichinchillae sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces." International Journal of Systematic and Evolutionary Microbiology 62, Pt_5 (2012): 1145–50. http://dx.doi.org/10.1099/ijs.0.032706-0.

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Strains of Gram-negative anaerobic rods were isolated from chinchilla (Chinchilla lanigera) faeces, and three strains, ST161T, ST33 and ST37T, were investigated taxonomically. Based on phylogenetic analyses and specific phenotypic characteristics, the three strains were allocated to the genus Bacteroides . Phylogenetic analyses of their 16S rRNA gene sequences revealed that strain ST161T formed a distinct line of descent, with highest sequence similarity to strain ST33 (98.7 %) and Bacteroides oleiciplenus JCM 16102T (97.7 %). High levels of DNA–DNA relatedness (79–89 %) were found between str

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Chang, Chia-Ning, Chia-Hsiang Yu, and Chih-Chien Wang. "A Decade of Pediatric CA-MRSA Surveillance in Northern Taiwan: Retrospective Resistance Analysis and Recent Genotypic Characterization." Microorganisms 13, no. 5 (2025): 1013. https://doi.org/10.3390/microorganisms13051013.

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Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of pediatric infections and has shown evolving molecular characteristics over time. This study aimed to investigate the phenotypic and genotypic features of MRSA isolates collected from pediatric patients at a tertiary medical center in northern Taiwan between 2011 and 2020. A total of 182 MRSA strains were analyzed for SCCmec types, PVL gene presence, antimicrobial susceptibility, multilocus sequence typing (MLST), and clonal relatedness using pulsed-field gel electrophoresis (PFGE). ST59/SCCmec Vt was the most prevalent geno

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Tsai, Chin-Shiang, Bo-Yang Tsai, Sung-Hsi Huang, et al. "Chlamydia trachomatisgenovariant L2b in men who have sex with men in Taiwan, 2020–2023." Sexually Transmitted Infections, April 24, 2025, sextrans—2024–056477. https://doi.org/10.1136/sextrans-2024-056477.

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ObjectivesLymphogranuloma venereum (LGV) caused byChlamydia trachomatisgenotypes L1–L3 has been resurging among men who have sex with men (MSM) and people with HIV (PWH) in Western countries. While historically attributed to tropical regions, rectal LGV has been rarely recognised in Asia, with Taiwan recently becoming the second Asian country to report cases.MethodsA multicentre, laboratory-based surveillance was conducted from January 2020 to December 2023 in Taiwan. Specimens were collected from MSM through syndromic testing and screening of high-risk populations.C. trachomatiswas identified

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Salmerón, Paula, Judit Serra-Pladevall, Martí Vall-Mayans, et al. "Genetic characterisation of lymphogranuloma venereum in Spain: a multicentre study." Sexually Transmitted Infections, July 24, 2024, sextrans—2023–056021. http://dx.doi.org/10.1136/sextrans-2023-056021.

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IntroductionLymphogranuloma venereum (LGV) is a sexually transmitted infection caused byChlamydia trachomatisgenotypes L1–L3. A combination of techniques with high discriminatory capacity such as multilocus sequence typing (MLST) and the analysis of theompAgene may be useful to determine the greater penetration of certain strains in transmission networks and their relationship with certain tropisms.AimThe aim of this study was to investigate the molecular epidemiology of LGV isolates from different regions of Spain.MethodsGenetic characterisation of LGV isolates detected in six hospitals from

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Giampetruzzi, Annalisa, Maria Saponari, Giuliana Loconsole, et al. "Genome-wide analysis provides evidence on the genetic relatedness of the emergent Xylella fastidiosa genotype in Italy to isolates from Central America." April 7, 2017. https://doi.org/10.1094/PHYTO-12-16-0420-R.

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Xylella fastidiosa is a plant pathogenic bacterium recently introduced in Europe that is causing decline in olive trees in the South of Italy. Genetic studies have consistently shown that the bacterial genotype recovered from infected olive trees belongs to the sequence type ST53 within subspecies pauca. This genotype, ST53, has also been reported to occur in Costa Rica. The ancestry of ST53 was recently clarified, showing it contains alleles which are monophyletic with those of subsp. pauca in South America. To more robustly determine the phylogenetic placement of ST53 within X. fastidiosa, w

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Annalisa, Giampetruzzi, Saponari Maria, Loconsole Giuliana, et al. "Genome-Wide Analysis Provides Evidence on the Genetic Relatedness of the Emergent Xylella fastidiosa Genotype in Italy to Isolates from Central America." April 7, 2017. https://doi.org/10.5281/zenodo.2536530.

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Xylella fastidiosa is a plant-pathogenic bacterium recently introduced in Europe that is causing decline in olive trees in the South of Italy. Genetic studies have consistently shown that the bacterial genotype recovered from infected olive trees belongs to the sequence type ST53 within subspecies pauca. This genotype, ST53, has also been reported to occur in Costa Rica. The ancestry of ST53 was recently clarified, showing it contains alleles that are monophyletic with those of subsp. pauca in South America. To more robustly determine the phylogenetic placement of ST53 within X. fastidiosa, we

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