Academic literature on the topic 'Stable isotope 15N'

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Journal articles on the topic "Stable isotope 15N"

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Demirev, Plamen A., and Catherine Fenselau. "Determination of Isotope-Enrichment Ratios in Proteins by High-Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry." European Journal of Mass Spectrometry 8, no. 2 (2002): 163–67. http://dx.doi.org/10.1255/ejms.485.

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Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry has been applied for determining the 13C and 15N stable isotope content of a 10 kDa protein molecule. Purified samples of modified human ubiquitin, expressed in E. coli grown in a 13C and 13N isotope-enriched medium, are electrosprayed in a 4.7 T FT-ICR instrument. The intensity profiles of the isotopically-resolved envelope of the molecular ions in different charge states are averaged. The experimental data are then compared to theoretically-generated isotopic profiles for the same molecule at varying ratios of 12C: 13C and
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Tea, Illa, Arnaud De Luca, Anne-Marie Schiphorst, et al. "Stable Isotope Abundance and Fractionation in Human Diseases." Metabolites 11, no. 6 (2021): 370. http://dx.doi.org/10.3390/metabo11060370.

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The natural abundance of heavy stable isotopes (13C, 15N, 18O, etc.) is now of considerable importance in many research fields, including human physiology. In fact, it varies between tissues and metabolites due to isotope effects in biological processes, that is, isotope discriminations between heavy and light isotopic forms during enzyme or transporter activity. The metabolic deregulation associated with many diseases leads to alterations in metabolic fluxes, resulting in changes in isotope abundance that can be identified easily with current isotope ratio technologies. In this review, we sum
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Alexander, Stuart A., Keith A. Hobson, Cheri L. Gratto-Trevor, and Antony W. Diamond. "Conventional and isotopic determinations of shorebird diets at an inland stopover: the importance of invertebrates and Potamogeton pectinatus tubers." Canadian Journal of Zoology 74, no. 6 (1996): 1057–68. http://dx.doi.org/10.1139/z96-117.

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We used gut-content and stable-isotope techniques to determine diets of shorebirds staging at a prairie wetland complex. Stable-isotope ratios for carbon (13C/12C) and nitrogen (15N/14N) varied greatly within and among prey types and shorebirds, depending on location within the complex. Both dietary techniques suggested that Long-billed Dowitchers (Limnodromus scolopaceus) and Stilt Sandpipers (Calidris himantopus) ate mostly invertebrates, whereas Hudsonian (Limosa haemastica) and Marbled godwits (Limosa fedoa) ate mainly Potamogeton pectinatus tubers. In comparison, the stable-isotope techni
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Keegan, William F., and Michael J. DeNiro. "Stable Carbon- and Nitrogen-Isotope Ratios of Bone Collagen Used to Study Coral-Reef and Terrestrial Components of Prehistoric Bahamian Diet." American Antiquity 53, no. 2 (1988): 320–36. http://dx.doi.org/10.2307/281022.

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Previous studies have demonstrated that stable carbon- and nitrogen-isotope ratios of bone collagen can be used to distinguish marine and terrestrial components of prehistoric human diet. However, when this method was first applied to prehistoric Bahamians, their bone-collagen nitrogen-isotope ratios were found to be outside the ranges observed for other coastal populations that ate substantial quantities of marine foods. This study examines in detail the distributions of stable carbon and nitrogen isotopes in Bahamian food chains. Our results indicate that the unique isotopic signature in bon
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Xie, Wancui, Min Li, Lin Song, et al. "15N Stable Isotope Labeling PSTs in Alexandrium minutum for Application of PSTs as Biomarker." Toxins 11, no. 4 (2019): 211. http://dx.doi.org/10.3390/toxins11040211.

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The dinoflagellate Alexandrium minutum (A. minutum) which can produce paralyticshellfish toxins (PSTs) is often used as a model to study the migration, biotransformation,accumulation, and removal of PSTs. However, the mechanism is still unclear. To provide a new toolfor related studies, we tried to label PSTs metabolically with 15N stable isotope to obtain 15N-PSTsinstead of original 14N, which could be treated as biomarker on PSTs metabolism. We then culturedthe A. minutum AGY-H46 which produces toxins GTX1-4 in f/2 medium of different 15N/Pconcentrations. The 15N-PSTs’ toxicity and toxin pro
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HAKVOORT, S. G. H., and O. SCHMIDT. "15N stable isotope labelling of slugs (Gastropoda: Pulmonata)." Annals of Applied Biology 141, no. 3 (2002): 275–81. http://dx.doi.org/10.1111/j.1744-7348.2002.tb00219.x.

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Overman, Nathanael C., та Donna L. Parrish. "Stable isotope composition of walleye: 15N accumulation with age and area-specific differences in δ13C". Canadian Journal of Fisheries and Aquatic Sciences 58, № 6 (2001): 1253–60. http://dx.doi.org/10.1139/f01-072.

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Stable nitrogen and carbon isotope ratios were measured for walleye (Stizostedion vitreum) collected across Lake Champlain, Vermont, to determine relationships between isotopic composition and diet, location of capture, length, weight, and age. Variation in δ13C values reflected area-specific differences in isotopic composition of organisms collected across the lake. A critical assumption in the application of isotope techniques is that a predictable relationship exists between the diet and isotopic composition of an organism. Our results indicate that isotopic fractionation factors may not be
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Kelly, Jeffrey F. "Stable isotopes of carbon and nitrogen in the study of avian and mammalian trophic ecology." Canadian Journal of Zoology 78, no. 1 (2000): 1–27. http://dx.doi.org/10.1139/z99-165.

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Differential fractionation of stable isotopes of carbon during photosynthesis causes C4 plants and C3 plants to have distinct carbon-isotope signatures. In addition, marine C3 plants have stable-isotope ratios of carbon that are intermediate between C4 and terrestrial C3 plants. The direct incorporation of the carbon-isotope ratio (13C/12C) of plants into consumers' tissues makes this ratio useful in studies of animal ecology. The heavy isotope of nitrogen (15N) is preferentially incorporated into the tissues of the consumer from the diet, which results in a systematic enrichment in nitrogen-i
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Schaumann, L., P. Galle, M. Thellier, and J. C. Wissocq. "Imaging the distribution of the stable isotopes of nitrogen 14N and 15N in biological samples by "secondary-ion emission microscopy"." Journal of Histochemistry & Cytochemistry 36, no. 1 (1988): 37–39. http://dx.doi.org/10.1177/36.1.3335768.

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Thanks to the "secondary-ion emission microscope" (CAMECA IMS 300), we have been able to image the distribution of the stable isotopes of nitrogen 14N and 15N in sections of plant roots (spatial resolution better than 1 micron), as well as to estimate the relative concentrations of these isotopes. The plants used (Lupinus spec.) originated from seeds with natural (i.e., 14N) nitrogen and had been fed for a few days with [15N]-nitrate before sampling. We have found in root sections of 6-day-old plants (prepared at 5 mm from the root tip) a clear-cut regionalization of the distribution of 15N be
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Kline, Jr., Thomas C., and T. Mark Willette. "Pacific salmon (Oncorhynchus spp.) early marine feeding patterns based on 15N/14N and 13C/12C in Prince William Sound, Alaska." Canadian Journal of Fisheries and Aquatic Sciences 59, no. 10 (2002): 1626–38. http://dx.doi.org/10.1139/f02-126.

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Nitrogen and carbon mass and stable isotope composition among cohorts of Pacific salmon (Oncorhynchus spp.) released from Prince William Sound, Alaska, hatcheries in 1994 varied widely, suggesting a range in early marine feeding patterns. Analyses consisted of whole-body stable carbon and nitrogen mass and stable isotope composition of selected release-date cohorts that had been identified by implanted coded wire tags (CWT). Nitrogen isotopic and mass shifts suggested that the initial protein pool within individual fish was replaced at different rates among cohorts. There was a notable differe
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Dissertations / Theses on the topic "Stable isotope 15N"

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Addison, Sarah Louise. "15N stable isotope probing of pulp and paper wastewaters." The University of Waikato, 2008. http://hdl.handle.net/10289/2399.

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Stable isotope probing (SIP) is an established technique that can be applied to identify the metabolically active micro-organisms within a microbial population. The SIP method utilises an isotopically-labelled substrate and PCR techniques to discern the members of a microbial community that incorporate the isotope into their DNA or RNA. The current literature gap around using 15N isotopes with RNA-SIP offers real potential and advantages for targeting and identifying active members from mixed communities involved in global biogeochemical nitrogen cycling. This study specifically investigated w
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Pears, Katrina. "Investigating nitrogen transfer between plants in agricultural grassland by using a 15N stable isotope labelling approach." Thesis, University of Bristol, 2018. http://hdl.handle.net/1983/bce1a3d6-218a-43aa-9033-788fc3432c72.

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The world’s population is predicted to reach 9.5 billion by 2050. This will put increasing pressure on already stretched food supplies. Previously, food supply has been increased by the use of synthetic fertilisers, particularly the use of nitrogen (N). However, fertilisers provide an unsustainable source of N, due to high energy demands for production as well as over-application and inadequate matching of fertiliser application to crop demand (synchrony). One solution to this global problem is the use of legumes, such as white clover (Trifolium repens L.), which are capable of fixing atmosphe
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Filho, Carlos Roberto de Sant\'Ana. "Síntese de uréia enriquecida com o isótopo 13C e/ou 15N." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/64/64135/tde-03082011-141751/.

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No presente trabalho foi sintetizada a 13CO(15NH2)2, enriquecida em 13C e/ou 15N, a partir da reação entre 13CO, 15NH3, S e CH3OH (meio líquido), sob baixa pressão e temperatura, com simultânea produção de H2S. A reação ocorreu em reator de aço inoxidável, revestido internamente com politetrafluoretileno. Inicialmente, adicionou-se S no interior do reator. Na sequência, esse foi fechado e, sob pressão reduzida (10-3 MPa) e temperatura de 4 ºC, transferiu-se o CH3OH, H2S, 15NH3 e 13CO. Logo após, o reator foi colocado em um sistema de aquecimento (100 ºC), sob agitação mecânica. Ao final do tem
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Werner, Amy. "Nitrogen release, tree uptake, and ecosystem retention in a mid-rotation loblolly pine plantation following fertilization with 15N-enriched enhanced efficiency fertilizers." Thesis, Virginia Tech, 2013. http://hdl.handle.net/10919/50975.

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Nitrogen is the most frequently limiting nutrient in southern pine plantations.  Previous studies found that only 10 to 25% of applied urea fertilizer N is taken up by trees.  Enhanced efficiency fertilizers could increase tree uptake efficiency by controlling the release of N and/or stabilize N.  Three enhanced efficiency fertilizers were selected as a representation of fertilizers that could be used in forestry: 1) NBPT treated urea (NBPT urea), 2) polymer coated urea (PC urea), and 3) monoammonium phosphate coated NBPT treated urea (MC NBPT urea). Urea, MC NBPT urea, and NBPT urea fertilize
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Fenilli, Tatiele Anete Bergamo. "Destino do nitrogênio (15N) do fertilizante em uma cultura de café." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/64/64132/tde-08052007-111834/.

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A cultura do café é um dos mais importantes agronegócios do Brasil e, apesar de suas práticas de manejo já estarem bem definidas, muitos aspectos da cultura ainda carecem esclarecimentos, destacando-se entre eles a nutrição nitrogenada do cafeeiro. Para isso foram conduzidos vários experimentos na cultura do café com adubação nitrogenada para se determinar o balanço do N no sistema solo-café-atmosfera, e melhor compreender a dinâmica do N dentro da planta. Os tratamentos constituíram de 5 sub-parcelas (repetições) com 9 plantas, as três centrais recebendo 280 kg ha-1 de N no 1º ano de experime
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Pereira, Leonardo Regis. "Dinâmica do nitrogênio amídico (15N-uréia) ou amoniacal (15N-aquamônia) no solo aplicado conjuntamente com o co-produto da produção do ácido glutâmico (Ajifer)." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/64/64134/tde-07122009-164411/.

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Com objetivo de estudar o efeito da adubação nitrogenada líquida aplicada sobre a palha em solo cultivado com cana-de-açúcar utilizando o co-produto da fabricação do ácido glutâmico (Ajifer), foi implantado um experimento em tubos de PVC de 100 mm no sistema confinado, simulando área com colheita mecanizada de cana-crua e área com colheita com despalha à fogo na fazenda experimental da Apta, Pólo Regional Centro Sul, em Piracicaba, SP. O fertilizante organomineral: Ajifer foi utilizado como veiculo para as fontes uréia (N-amídico) ou aquamônia (N-amoniacal) marcadas com 15N. Este experimento
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Smyth, Patrick. "Studying the Temporal Dynamics of the Gut Microbiota Using Metabolic Stable Isotope Labeling and Metaproteomics." Thesis, Université d'Ottawa / University of Ottawa, 2021. http://hdl.handle.net/10393/42344.

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The gut microbiome and its metabolic processes are dynamic systems. Surprisingly, our understanding of gut microbiome dynamics is limited. Here we report a metaproteomic workflow that involves protein stable isotope probing (protein-SIP) and identification/quantification of partially labeled peptides. We also developed a package, which we call MetaProfiler, that corrects for false identifications and performs phylogenetic and time series analysis for the study of microbiome dynamics. From the stool sample of five mice that were fed with 15-N hydrolysate from Ralstonia eutropha, we identified 1
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Bruno, Isabeli Pereira. "Eficiência da aplicação de ureia em fertirrigação de cafeeiros irrigados por pivô central, utilizando o traçador 15N." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-17032011-135748/.

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A cultura do cafeeiro é muito importante para a economia agrícola brasileira e em anos recentes tem avançado para o cerrado do oeste da Bahia. Esta região apresenta condições de solo e clima diferentes daquelas onde a cafeicultura já está estabelecida e, portanto, necessita de reavaliações no seu manejo, principalmente quanto à adubação nitrogenada, visto que vem sendo aplicadas doses muito maiores do que aquelas usuais em outras zonas cafeicultoras. A fertirrigação é muito usada nessa região, permitindo a divisão da dose de nitrogênio (N) em várias parcelas, o que comumente diminui as perdas
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Johns, Noel. "Stable Isotopes and Multiple Tissue Analysis: Reconstructing Life Histories for Individuals from Dakhleh Oasis, Egypt." Master's thesis, University of Central Florida, 2012. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5325.

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Stable isotope analysis is often used to evaluate elements of the lives of past peoples, such as diet and health status, at a societal level. Analysis at an individual level is exceptionally rare, and has not been conducted using a variety of tissues representing both early life and life approximate to death. In this study, [delta]13C and [delta]15N isotope signatures are used to create life histories for single individuals from Romano-Christian period Kellis 2 cemetery in the Dakhleh Oasis, Egypt. Samples are obtained from several different tissues, including tooth dentin, bone collagen, hair
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Coletta, Luciana Della. "Estudo da fixação biológica do nitrogênio em leguminosas (família Fabaceae) arbóreas tropicais através do enriquecimento isotópico do 15N." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/64/64135/tde-25112010-162659/.

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A família Fabaceae abundante nos trópicos, desperta grande interesse para o uso em recuperação de áreas degradas, já que em sua maioria, são lenhosas e perenes, se adaptam aos mais diversos ecossistemas brasileiros e são capazes de fazer associações simbiônticas com bactérias pertencentes ao gênero Rhizobium, que fixam nitrogênio atmosférico fornecendo-o para a planta em uma forma reativa. Dentro deste contexto, o presente estudo avaliou em um experimento em casa de vegetação como a fixação biológica de nitrogênio em três espécies pertencentes a família Fabaceae variou em função da adição de n
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Books on the topic "Stable isotope 15N"

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Segschneider, Hans-Josef. Untersuchungen zur Aufnahme und zum Einbau von anthropogenen Stickoxiden (NOx) durch Sonnenblumen und Mais mittels 15N-Isotopen-Markierung. Forschungszentrum Jülich GmbH, Zentralbibliothek, 1994.

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Porter, L. K. 15N techniques and analytical procedures: Indo/U.S. science and technology initiative. 1992.

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Metabolic Analysis Using Stable Isotopes. Elsevier, 2015. http://dx.doi.org/10.1016/s0076-6879(15)x0013-9.

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Book chapters on the topic "Stable isotope 15N"

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Buckley, Daniel H. "Stable Isotope Probing Techniques Using 15N." In Stable Isotope Probing and Related Technologies. ASM Press, 2014. http://dx.doi.org/10.1128/9781555816896.ch7.

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Angel, Roey. "Stable Isotope Probing Techniques and Methodological Considerations Using 15N." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9721-3_14.

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Unkovich, Murray, and John S. Pate. "Assessing N2 Fixation in Annual Legumes using 15N Natural Abundance." In Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9841-5_6.

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Fillery, Ian R. P., and Sylvie Recous. "Use of Enriched 15N Sources to study Soil N Transformations." In Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9841-5_9.

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Peoples, Mark B., Brian Palmer, and Robert M. Boddey. "The Use of 15N to study Biological Nitrogen Fixation by Perennial Legumes." In Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9841-5_7.

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Charteris, Alice Fiona. "Compound-Specific Amino Acid 15N Stable Isotope Probing of Nitrogen Assimilation by the Soil Microbial Biomass Using Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry." In 15N Tracing of Microbial Assimilation, Partitioning and Transport of Fertilisers in Grassland Soils. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-31057-8_3.

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McNeill, Ann. "Stable Isotope Techniques using Enriched 15N and 13C for Studies of Soil Organic Matter Accumulation and Decomposition in Agricultural Systems." In Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9841-5_10.

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Otto, Andreas. "Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8695-8_13.

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Shearer, G., and D. H. Kohl. "Estimates of N2 Fixation in Ecosystems: The Need for and Basis of the 15N Natural Abundance Method." In Stable Isotopes in Ecological Research. Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3498-2_20.

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Virginia, R. A., W. M. Jarrell, P. W. Rundel, G. Shearer, and D. H. Kohl. "The Use of Variation in the Natural Abundance of 15N to Assess Symbiotic Nitrogen Fixation by Woody Plants." In Stable Isotopes in Ecological Research. Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3498-2_21.

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Conference papers on the topic "Stable isotope 15N"

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Yang, Joon S., Teresa W.-M. Fan, Andrew N. Lane, Jason A. Brandon, and Richard M. Higashi. "Abstract PO-006: Tracking S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) biosynthesis pathway using stable isotope-resolved metabolomics (SIRM)." In Abstracts: AACR Special Virtual Conference on Epigenetics and Metabolism; October 15-16, 2020. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.epimetab20-po-006.

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Willems, M., P. Luycx, R. Gilis, C. I. Renard, H. Reyniers, and J. M. Cuchet. "The HRA/SOLARIUM Project: Processing of Historical Waste." In ASME 2003 9th International Conference on Radioactive Waste Management and Environmental Remediation. ASMEDC, 2003. http://dx.doi.org/10.1115/icem2003-4732.

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Starting in 2003, Belgoprocess will proceed with the treatment and conditioning of some 200 m3 of widely varying high- and medium-level waste from earlier research and development work, to meet standard acceptance criteria for later disposal. The gross volume of primary and secondary packages amounts to 2,600 m3. The waste has been kept in decay storage for up to 30 years. The project was started in 1997. Operation of the various processing facilities will take 7–8 years. The overall volume of conditioned waste will be of the order of 800 m3. All conditioned waste will be stored in appropriate
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Willems, M., L. Krieckemans, P. Luycx, and A. Meeus. "The HRA/Solarium Project: Processing of Widely Varying High- and Medium-Level Waste." In ASME 2001 8th International Conference on Radioactive Waste Management and Environmental Remediation. American Society of Mechanical Engineers, 2001. http://dx.doi.org/10.1115/icem2001-1209.

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Abstract Starting in 2002, Belgoprocess will proceed with the treatment and conditioning of some 200 m3 of widely varying high- and medium-level wastes from earlier research and development work, to meet standard acceptance criteria for later disposal. The gross volume of primary and secondary packages amounts to 2,600 m3. The wastes have been kept in decay storage for up to 30 years. The project was started in 1998. Operation of the various processing facilities will take 7–8 years. The overall volume of conditioned waste will be of the order of 800 m3. All conditioned waste will be stored in
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Reports on the topic "Stable isotope 15N"

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Guss, W. The plasma centrifuge: A compact, low cost, stable isotope separator. Phase 2 final technical report, September 15, 1991--September 14, 1995. Office of Scientific and Technical Information (OSTI), 1996. http://dx.doi.org/10.2172/345051.

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Ballentine, C. J., Alexander N. Halliday, and B. Sherwood Lollar. Conbined noble gas and stable isotope constraints on nitrogen gas sources within sedimentary basins. Final report for period 15 March 1996 - 14 March 1999 extended to 14 March 2000. Office of Scientific and Technical Information (OSTI), 2001. http://dx.doi.org/10.2172/810582.

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The plasma centrifuge (A compact, low cost, stable isotope separator). Progress report, [September 15, 1991--September 14, 1992]. Office of Scientific and Technical Information (OSTI), 1992. http://dx.doi.org/10.2172/10160001.

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