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Journal articles on the topic 'Stains'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Iyare, G. I., S. Ehiremen, and N. T. Omorodion. "Demonstrating the staining ability of <i>Zingiber officinale</i> and <i>Curcuma longa</i> as replacement to Eosin Y in EA50." Scientia Africana 23, no. 3 (2024): 409–16. http://dx.doi.org/10.4314/sa.v23i3.36.

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Nicholas Papanicolaou developed the Pap stain, a combination of three major stains used for cervical smears. Eosin-Azure (EA50), one of these stains, includes Eosin Y, Light Green SF, and Bismarck brown. This study explores the use of Curcuma longa extract as a substitute for Eosin Y in EA50. Cervical smears from premenopausal women were collected and stained using both the standard Papanicolaou stain and a modified version where Eosin Y was replaced with Curcuma longa extract. The modified stain's effectiveness was assessed based on the quality of staining of superficial cells. The modified s

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Blagojević, Nikola, Igor Mihajlović, Jovana Džunić та ін. "Abstract LB348: ProMiSЕ: Probabilistic multi-stain estimator for color separation in multiplexed brightfield histopathology images". Cancer Research 85, № 8_Supplement_2 (2025): LB348. https://doi.org/10.1158/1538-7445.am2025-lb348.

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Abstract Introduction: Brightfield histopathology uses chromogenic staining to assign distinct colors to different specimen elements. Multiplexing biomarker staining on the same slide has valuable advantages, including the ability to capture the spatial distribution of different cell populations and tissue structures. Color separation is a computational method that separates contributions from each chromogen. It enables the analysis of individual stain expressions, and serves as a crucial preprocessing step in numerous histopathology image analysis tasks (e.g. nuclear segmentation, stain norma

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3

Tallman, B., O. T. Tan, Shawn Trainor, et al. "Location of Port-Wine Stains and the Likelihood of Ophthalmic and/or Central Nervous System Complications." Pediatrics 87, no. 3 (1991): 323–27. http://dx.doi.org/10.1542/peds.87.3.323.

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Of 310 patients with port-wine stains, 68% had more than one dermatome involved; 85% had unilateral and 15% had a bilateral distribution of their port-wine stain. At the time of examination, 8% of all patients with trigeminal port-wine stains had evidence of eye and/or central nervous system (CNS) involvement. Extensive involvement, with port-wine stain over the trunk and extremities as well as the head and neck, was observed in 12%. Patients who did not have port-wine stains on the areas served by branches V1 and V2 of the trigeminal nerve had no signs or symptoms of eye and/or CNS involvemen

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4

Brady, John G., Gordon E. Schutze, Robert Seibert, Hazel V. Horn, Barbara Marks, and David M. Parham. "Detection of Mycobacterial Infections Using the Dieterle Stain." Pediatric and Developmental Pathology 1, no. 4 (1998): 309–13. http://dx.doi.org/10.1007/s100249900044.

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Retrospective review comparing the modified Dieterle stain with standard acid-fast stains was performed on seven surgical pathology cases that contained culture-positive mycobacteria infections. Tissues examined comprised cervical and submandibular lymph nodes and soft tissues of the face and chest. Modified Dieterle staining was performed on paraffin-embedded tissue sections, and the results were compared with those of hematoxylineosin stains and auramine-rhodamines and carbol-fuchsin acid-fast stains. The acid-fast stain showed organisms in three of seven cases on initial review and five of

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Woods, G. L., and D. H. Walker. "Detection of infection or infectious agents by use of cytologic and histologic stains." Clinical Microbiology Reviews 9, no. 3 (1996): 382–404. http://dx.doi.org/10.1128/cmr.9.3.382.

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A wide variety of stains are useful for detection of different organisms or, for viruses, the cytopathologic changes they induce, in smears prepared directly from clinical specimens and in tissue sections. Other types of stains, such as hematoxylin and eosin, are used routinely to stain tissue sections and are most valuable for assessing the immunologic response of the host to the invading pathogen. In many cases, the pattern of inflammation provides important clues to diagnosis and helps to guide the selection of additional "special" stains used predominantly for diagnosis of infectious disea

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6

Pandey, Neetu, Rajendra Patil, Udita Singh, Himani Singh, Satarupa Debnath, and Deeksha Gahlot. "Evaluation of lugol’s iodine as a screening tool for identification of dried saliva stains." International Dental Journal of Student's Research 11, no. 1 (2023): 1–5. http://dx.doi.org/10.18231/j.idjsr.2023.001.

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The evidential value of saliva in stains encountered in crime investigations can be of considerable importance. Whether the stains are a result of expectoration or a consequence of some sexual or violent act resulting in saliva being mixed with other body fluids, the investigating forensic expert is faced with two fundamental problems: localization of stain and visualization of stain. Visualization of saliva is problematic unlike other body fluids like blood which has its own internal marker (i.e., hemoglobin) or semen that can be easily identified by acid phosphatase method.: Evaluate of lugo

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7

Zubir, Moondra, Ayi Darmana, Marini Damanik, et al. "Bleach Effectively in Removes The Stubborn Stains." Indonesian Journal of Chemical Science and Technology (IJCST) 3, no. 1 (2020): 20. http://dx.doi.org/10.24114/ijcst.v3i1.18312.

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Along with the development of science and technology, more and more types of manufactured goods produced to meet our needs we need to know that among these materials there are dangerous or toxic, therefore it is very important for us to know the type, nature, usefulness, as well as the dangers of every chemical that we use at home As we know, all kinds of objects that are around us are actual material, all material consists of chemicals but, in our daily lives we commonly use the term material instead of chemicals. Clothing that we use every day will be prone to stains so it is important to kn

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Menon, Gayathry S., Amit K. Walvekar, B. Radhika, K. B. Uthappa, and Sujitha S. Golla. "Power of Amplified Light Against Chromogenic Bacterial Stains - A Case Report." Journal of Multidisciplinary Dental Research 9, no. 2 (2023): 64–66. http://dx.doi.org/10.38138/jmdr/v9i2.22.46.

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The treatment of black stains has always been a grey area for the dentists to deal with due to its recurrence. The more effective treatments in current literature are lactoferrin, diode laser, laser Nd:YAG and phototherapy. An attempt was made to remove the stain using low level laser therapy with the similar principle of lethal laser photosensitization and photodynamic therapy without the use of any dye. Our study showed a positive result with the reduction of black stains over a period of 1 year. Hence, the use of LLLT could be considered as one of the adjunct treatment modality for chromoge

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Pratha, Dr Sruthy, Dr Prathap M. S, and Dr Nihala Sali. "Relationship between Black Chromogenic Stains and Hemoglobin Levels." South Asian Research Journal of Oral and Dental Sciences 4, no. 4 (2022): 37–41. http://dx.doi.org/10.36346/sarjods.2022.v04i04.001.

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Background: Occurance of black discolouration of teeth is very common and a clinical challenge in the present day dentistry. Stains associated with chromogenic bacteria have a high rate of recurrence even after thorough scaling and polishing procedures. The mechanism of stain formation is still not understood clearly though its chemical composition is mainly iron suiphide and bacteria like actinomyces have been identified in plaque samples of these patients which have the ability to form iron sulphides. The iron content of saliva is known to increase in iron deficiency anemia and iron overload

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10

Belkacem, Oussama. "USE OF ROUTINE SPECIAL STAINS FOR GASTRIC AND ESOPHAGEAL BIOPSIES." EPH - International Journal of Medical and Health Science 4, no. 1 (2018): 80–84. http://dx.doi.org/10.53555/eijmhs.v4i1.29.

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Problem: Special stains such as the Giemsa, the Alcian blue (AB) and the Periodic Acid Schiff (PAS) are widely used for gastric and/or esophageal biopsies for the diagnosis of Helicobacter pylori (H. pylori) and intestinal metaplasia (IM). The purpose of our study was to determine if these stains are actually needful Methods: We retrospectively studied 209 gastric and esophageal biopsies. We evaluated the H. pylori status on hematoxylin and eosin (H&E) stained slides and the presence of IM and finally we examined the special stains. Results: 23% of cases were H. pylori-positive

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Bradford, Jolene A., Pam Whitney, Timothy Huang, et al. "Novel Vybrant® DyeCycle ™ Stains Provide Cell Cycle Analysis in Live Cells Using Flow Cytometry with Violet, Blue, and Green Excitation." Blood 108, no. 11 (2006): 4234. http://dx.doi.org/10.1182/blood.v108.11.4234.4234.

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Abstract Flow cytometric approaches can resolve cell position in the cell cycle based on DNA content, and these analyses are widely used in the study of cell growth, cell cycle regulation, and oncology. These applications require dyes that bind to DNA in a stoichiometric manner and which, with the exception of some UV-excited dyes like Hoechst 33342, require fixation, permeabilization and RNAse treatment for reproducible results. The Vybrant® DyeCycle™ stains are DNA-selective, cell membrane-permeant dyes that show greatly-enhanced fluorescence when bound to DNA. DyeCycle violet stain can be e

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CHICK, ANDREW I. R. "Stains for entomological microtechnique: simple stains for whole mounts and dissection." Zootaxa 4790, no. 3 (2020): 447–72. http://dx.doi.org/10.11646/zootaxa.4790.3.2.

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Slide mounted entomological specimens often require the aid of contrast techniques to improve the clarity of morphological characteristics. Methods can involve the use of techniques such as Phase contrast, Dark field or differential interference contrast microscopy (DIC), however where an entomologist may only have access to simple brightfield microscopy chemical staining of the specimen may be used to improve contrast. For whole mounts of entomological specimens, a single stain, occasionally two, are often used, in comparison to histological sections that often employ multiple stains in compl

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13

Veresova, Nina M. "EMBRACE THE STAIN: EXPLORING THE POTENTIAL OF FAILURE IN TEXTILE DESIGN." Articult, no. 4 (December 2024): 84–98. https://doi.org/10.28995/2227-6165-2024-4-84-98.

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This paper addresses the issue of accidental stains on textiles as opposed to intentional printing and dyeing practices. The subject of study is individual and collaborative craft design strategies which are centred around the stain and its treatment. The nature and types of stains as well as varied opinions and interpretations that reflect social constructions, norms, and values around it are observed. The creative potential of the stain for textile artists and designers who experiment with print and dye, delving into the possibilities of stain and questioning the borderlines between those ar

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14

Mohammed Abdalla MA, Osman Amir, and Elnazir Mohammed E. "Detection of Helicobacter pylori (H. pylori) by histochemical stains in gastric biopsy comparing to immunohistochemistry." World Journal of Advanced Research and Reviews 15, no. 2 (2022): 155–59. http://dx.doi.org/10.30574/wjarr.2022.15.2.0793.

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Aim: A reliable diagnosis of Helicobacter pylori (H. pylori) is important in clinical practice and research. The aim of this study was to compare the sensitivity and specificity of Giemsa stain with hematoxylin and eosin (H&E), Toluidine blue, Gimenez and Warthin-Starry stain in detection of Helicobacter pylori (H. pylori) in gastric biopsy and also with immunohistochemistry (IHC) stain in detection of H. pylori organism in gastric biopsy. Method: A retrospective cross-sectional included 200 formalin-fixed paraffin-embedded (FFPE) gastric biopsies of age between 25 to 80 years in histopath

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Alturkistani, Hani A., Faris M. Tashkandi, and Zuhair M. Mohammedsaleh. "Histological Stains: A Literature Review and Case Study." Global Journal of Health Science 8, no. 3 (2015): 72. http://dx.doi.org/10.5539/gjhs.v8n3p72.

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<p>The history of histology indicates that there have been significant changes in the techniques used for histological staining through chemical, molecular biology assays and immunological techniques, collectively referred to as histochemistry. Early histologists used the readily available chemicals to prepare tissues for microscopic studies; these laboratory chemicals were potassium dichromate, alcohol and the mercuric chloride to harden cellular tissues. Staining techniques used were carmine, silver nitrate, Giemsa, Trichrome Stains, Gram Stain and Hematoxylin among others.</p> &

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Villalobos-Tinoco, Jose, Carlos A. Jurado, Silvia Rojas-Rueda, et al. "Micro- and Macroabrasion in the Esthetic Zone: A Narrative Review and Case Study." Dentistry Journal 13, no. 5 (2025): 183. https://doi.org/10.3390/dj13050183.

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Background: Micro- and macroabrasion represent a minimally invasive treatment approach for stained teeth in the esthetic zone. Diagnosing the type of stain is crucial for selecting the appropriate treatment approach. These clinical procedures involve several meticulous steps that may be confusing for less experienced clinicians. Methods: The objective of this article is to provide an updated review of the literature on the clinical procedures for micro- and macroabrasion and to present a clinical case in which a minimally invasive macroabrasion procedure was applied to treat a female patient s

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17

Warsi, Sumbul, Fatma Lubna, Medha Mani Gupta, and Ruquiya Afrose. "Histoplasmosis: A case report." IP Archives of Cytology and Histopathology Research 9, no. 2 (2024): 121–25. http://dx.doi.org/10.18231/j.achr.2024.025.

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Histoplasmosis is common in certain regions of America, Asia and Africa. Cutaneous manifestation of Histoplasma capsulatum shows a wide spectrum of lesions. Histopathology plays a very important role in the detection and confirmation of diagnosis. Special fungus stains such as GMS (Gomori’s methenamine silver stain) and PAS (Periodic acid Schiff) stains can be used for its study.

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Zarrin-Khameh, Neda, and Kim S. Kaye. "Alveolar Soft Part Sarcoma." Archives of Pathology & Laboratory Medicine 131, no. 3 (2007): 488–91. http://dx.doi.org/10.5858/2007-131-488-asps.

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Abstract This article provides an overview of the pathology of alveolar soft part sarcoma, focused on its morphology, special stains useful in diagnosis, and the clinical and radiographic features of the disease. Alveolar soft part sarcoma is a rare neoplasm of unknown histogenesis with poor prognosis. Although there are several immunohistochemical stains available to help reach the diagnosis, the morphology of the tumor should be considered the main diagnostic feature. The periodic acid–Schiff stain is the best single stain that supports the diagnosis.

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Jha, Prasanna Kumar, and Satyendra Kumar Singh. "A Rare Presentation of Acquired Port Wine Stain in an Elderly Male." Nepalese Medical Journal 4, no. 1 (2021): 457–58. http://dx.doi.org/10.3126/nmj.v4i1.36714.

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Acquired port wine stain though an uncommon entity that develops later in life, resembles congenital port wine stain morphologically and histologically. Congenital port wine stains are vascular lesions caused by progressive ectasia of blood vessels which is located in the vascular plexus of the dermis. Congenital port-wine stains may be associated with Sturge Weber syndrome causing neurological and eye abnormalities such as glaucoma. Here we report a 60-year-old male presenting with a complaint of asymptomatic reddish patches over the nose for 15 years.

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20

Cuc, Stanca, Codruta Sarosi, Ioan Petean, Amalia Moldovan, Cecilia Bacali, and Sorin Claudiu Man. "Whitening Efficiency of Papain and Bromelain Gels in Removing Dental Stains." Gels 11, no. 2 (2025): 100. https://doi.org/10.3390/gels11020100.

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This study aimed to evaluate the micro-nanostructure and color changes of dental enamel after treatment with new gel formulations containing papain or bromelain. Eighty caries-free, extracted human teeth were randomly divided into two groups (n = 40) and stained by immersion in either coffee or Tedi juice for 4 h daily over five consecutive days. After staining, the samples were washed and stored in artificial saliva at 37 °C. The stained samples were then treated with four different whitening gels (GC, G1, G2, and Opalescence 15%) for 4 h daily. Following each treatment, the samples were rins

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Saba, C., M. Solidani, F. Berlutti, A. Vestri, L. Ottolenghi, and A. Polimeni. "Black stains in the mixed dentition: A PCR microbiological study of the etiopathogenic bacteria." Journal of Clinical Pediatric Dentistry 30, no. 3 (2006): 219–24. http://dx.doi.org/10.17796/jcpd.30.3.q1561155x22u0774.

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The aim of this work is to emphasize that particular stains on the third cervical of the buccal and lingual surfaces in mixed dentition, called "black stain." Previous research showed the microbiological etiology of this discoloration by chromogen bacterias. Our study shows bacteria spp involved in stains by means of PCR process and electrophoresis gel on the agarose medium. Sample was formed by 100 subject with black stain and 100 control subjects stain-free.A statistical analysis (SPSS 10.0) using X2 was performed in this study. Porphyromonas gingivalis and Prevotella melaninogenica, were no

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OPPONG, DAVID, and BIRDELL H. SNUDDEN. "Comparison of Acridine Orange Staining Using Fluorescence Microscopy with Traditional Methods for Microbiological Examination of Selected Dry Food Products." Journal of Food Protection 51, no. 6 (1988): 485–88. http://dx.doi.org/10.4315/0362-028x-51.6.485.

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A comparison was made between the acridine orange stain, gram stain and methylene blue stain for direct microscopic counts (DMC) of microorganisms in gravy mixes, spices, cocoa products and baby foods. Bacteria were detected in 96% (45/47) of the samples stained with acridine orange, 64% (30/47) for the gram stain and 66% (31/47) for the methylene blue stain. In most instances, acridine-orange smears showed higher numbers of bacteria than the traditional stains. The staining quality of the acridine orange was better than the conventional stains with bacteria, yeast cells, and mold hyphae fluor

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Choudhary, Prabesh, Niraj Nepal, Rashmita Bhandari, Ritu Baral, Oshan Shrestha, and Sarita Choudhary. "Comparative study of conventional Papanicolaou and ultrafast Papanicolaou stains in fine needle aspiration samples and body fluids." Journal of Pathology of Nepal 14, no. 2 (2024): 2209–12. https://doi.org/10.3126/jpn.v14i2.71320.

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Background: Ultra Papanicolaou stain was introduced as a hybrid of Romanowsky and Papanicolaou stains. It enhances the quality and reduces the time. Materials and Methods: Five hundred and ninety-five fine needle aspiration and body fluid samples were collected. The fine needle aspiration procedure was performed by standard method; smears were fixed in 95% propanol and stained with conventional and ultra Papanicolaou stains. Four parameters were considered and scored (background, cell morphology, nuclear staining, and overall staining pattern). Results: The quality of ultra Papanicolaou stain

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Nathoo, S. A., and A. Gaffar. "Studies on Dental Stains Induced by Antibacterial Agents and Rational Approaches for Bleaching Dental Stains." Advances in Dental Research 9, no. 4 (1995): 462–70. http://dx.doi.org/10.1177/08959374950090041801.

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Extrinsic stain resides in the dental pellicle and can be caused by introduction of chromogenic materials or therapeutic agents into the oral cavity. In contrast, intrinsic tooth stain is found within the tooth structure and can be caused by a variety of agents, including hematological and developmental abnormalities and drugs such as tetracycline. The mechanisms of extrinsic stain formation differ with respect to the causative agent. For example, stain induced by chlorhexidine (CH) can be explained by an increased rate in the non-enzymatic browning reactions occurring at the pellicle surface,

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Clark, Penny, Traci Kurtzer, and Patrick Duff. "Role of Bacterial Vaginosis in Peripartum Infections." Infectious Diseases in Obstetrics and Gynecology 2, no. 4 (1994): 179–83. http://dx.doi.org/10.1155/s106474499400061x.

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Objective: The purpose of this prospective investigation was to determine if the presence of bacterial vaginosis (BV) at the time of delivery was associated with the development of maternal and neonatal infection.Methods: Vaginal fluid was collected from 390 laboring patients. Smears of the vaginal secretions were gram stained, and slides were scored and interpreted as normal, intermediate, and BV based on Gram's stain criteria. Results of the Gram's stains were correlated with the clinical diagnoses of chorioamnionitis, endometritis, and neonatal sepsis.Results: Eighty-eight percent of patien

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Wallis, Louise, Catherine Hitchcock, Dennis McNevin, and Jennifer Raymond. "Source Level Attribution: DNA Profiling from the ABAcard® HemaTrace® Kit." Forensic Sciences 1, no. 3 (2021): 116–29. http://dx.doi.org/10.3390/forensicsci1030011.

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ABAcard® HemaTrace® kits have been used for crime scene stains for confirmation of human blood for many years. However, when the stain is too small to allow for separate testing, confirmatory testing may be forgone to preference DNA analysis. This can lead to court challenges as to the biological source and therefore probative value of the DNA profile. This research aimed to develop a protocol for DNA analysis of a minute blood stain subsequent to HemaTrace® testing. Stains were collected and subjected to HemaTrace® testing. Swabs were then removed from the HemaTrace® buffer solution and proce

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Sun, Sheng, Jianyuan Wang, Yuan Ning, and Menglun Zhang. "Air-coupled piezoelectric micromachined ultrasonic transducers for surface stain detection and imaging." Nanotechnology and Precision Engineering 5, no. 1 (2022): 013004. http://dx.doi.org/10.1063/10.0009632.

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This paper proposes an air-coupled piezoelectric micromachined ultrasonic transducer (PMUT) for detection and imaging of surface stains. A 508 kHz PMUT array is designed, fabricated, and characterized in terms of its electrical and acoustic properties, and it is used in a pulse echo validation test. Imaging of stains on metal blocks is successfully demonstrated. Compared with existing optical methods for stain detection, the proposed approach can work in a dark environment without color requirements. This work provides a new and promising route for the development of miniaturized stain detecti

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Kropat, Marcel, Paul Frederik Laleicke, and Juan Jose Acosta. "Towards Inline Prediction of Color Development for Wood Stained with Chemical Stains Using Near-Infrared Spectroscopy." Forest Products Journal 72, no. 2 (2022): 130–39. http://dx.doi.org/10.13073/fpj-d-22-00021.

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Abstract The chemical composition of wood determines the color development when applying chemical stains to the surface of wood. However, different species and individuals from the same species can show variations in the chemical composition, resulting in the risk of nonuniform color development in industrial staining processes between different batches of wood. In the present study, near-infrared (NIR) models were developed to predict wood specimen color development after applying three different concentrations of the chemical stains iron acetate and sodium bicarbonate. The modeling dataset i

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Mohammed, Abdalla MA, Amir Osman, and Mohammed E. Elnazir. "Detection of Helicobacter pylori (H. pylori) by histochemical stains in gastric biopsy comparing to immunohistochemistry." World Journal of Advanced Research and Reviews 15, no. 2 (2022): 155–59. https://doi.org/10.5281/zenodo.7756057.

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<strong>Aim</strong>: A reliable diagnosis of <em>Helicobacter pylori </em>(<em>H. pylori</em>) is important in clinical practice and research. The aim of this study was to compare the sensitivity and specificity of Giemsa stain with hematoxylin and eosin (H&E), Toluidine blue, Gimenez and Warthin-Starry stain in detection of <em>Helicobacter pylori </em>(<em>H. pylori</em>) in gastric biopsy and also with immunohistochemistry (IHC) stain in detection of <em>H. pylori</em> organism in gastric biopsy. <strong>Method</strong>: A retrospective cross-sectional inc

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Aprilina, Reza Asri, Neneng Nurjannah, Nining Ningrum, and Tri Widyastuti. "The Relationship Of Coffee Consumption Habits With The Occurence Of Stain In Community In Sirap Village." Jurnal Terapi Gigi dan Mulut 2, no. 2 (2023): 68–72. http://dx.doi.org/10.34011/jtgm.v2i2.1401.

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Stain is a staining on the teeth caused by the attachment of food, drink, or other content, such as coffee. The habit of consuming coffee is one of the factors that cause stains due to caffeine contained in coffee. The purpose of this study was to determine the relationship between coffee consumption habits and the occurrence of stains on teeth. This type of research is analytic with a cross sectional research design which was carried out in March 2022 on 50 respondents with a sampling technique using purposive sampling technique. The habit of consuming coffee was obtained through interv

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Shobana G., Muthu Karuppaiah R., Bharath Kumar Garla, Taranath M., and Palanivel Pandian R. "Effect of Whitening Toothpastes on Extrinsic Dental Stains." Journal of Advanced Oral Research 10, no. 1 (2019): 19–23. http://dx.doi.org/10.1177/2320206819834411.

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Aim and objectives: To compare the effectiveness of the stain removing property of the whitening toothpastes (silica [Colgate Visible White], silica, papain and bromelain [Whitospark], and silica and calcium carbonate [Snowdent] containing toothpastes) on extrinsic dental stains and to assess the lasting of tooth whitening effect produced by the whitening toothpastes. Materials and methods: It is a randomized, concurrent parallel arm, non-invasive, controlled trial designed to compare the effectiveness of the whitening toothpastes on reducing extrinsic dental stains. Parametric t-test was used

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Zhou, Pingping, Miaole Hou, Shuqiang Lv, Xuesheng Zhao, and Wangting Wu. "Virtual Restoration of Stained Chinese Paintings Using Patch-Based Color Constrained Poisson Editing with Selected Hyperspectral Feature Bands." Remote Sensing 11, no. 11 (2019): 1384. http://dx.doi.org/10.3390/rs11111384.

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Stains, as one of most common degradations of paper cultural relics, not only affect paintings’ appearance, but sometimes even cover the text, patterns, and colors contained in the relics. Virtual restorations based on common red–green–blue images (RGB) which remove the degradations and then fill the lacuna regions with the image’s known parts with the inpainting technology could produce a visually plausible result. However, due to the lack of information inside the degradations, they always yield inconsistent structures when stains cover several color materials. To effectively remove the stai

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D'Orio, Eugenio, Francesco Mercante, and Teresa Di Sarno Giusto. "New Methodology for Non-destructive Identification of Body Fluid for Forensic Purposes." Arab Journal of Forensic Sciences and Forensic Medicine 6, Special Issue (2024): 137–44. http://dx.doi.org/10.26735/axem4254.

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The purpose of this research study was to test and subsequently validate a method based on the identification of biological fluids by nondestructive analysis, attributing phenotypic characteristics to biological stains. Specifically, a new methodology for non-destructive forensic histological analysis using Alternative Light Source 450 nm + orange filters was developed and validated - also testing its analytical efficiency and inherent degree of probabilistic error. The validation process has been carried out through the creation of a standard database of known samples, in which were included

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Al-kharouf, I., M. Stoieva, and A. Hamza. "Immunoreactivity of Histiocytes to OCT4; a diagnostic pitfall." American Journal of Clinical Pathology 160, Supplement_1 (2023): S44—S45. http://dx.doi.org/10.1093/ajcp/aqad150.100.

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Abstract Introduction/Objective Immunohistochemical stains are supposed to make a Pathologists’ life easier; however, interpretation of some stains in isolation can be a diagnostic pitfall. Immunoreactivity of paraganglia to OCT4 stain is a known pitfall for this stain when evaluating retroperitoneal lymph node dissection specimens for germ cell tumour metastasis. The purpose of this report is to highlight another pitfall of the OCT4 stain, albeit in a similar clinical scenario. Methods/Case Report A 49-year-old male with a history of left testicular seminoma metastatic to periaortic, retroper

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Sharma, Anurag, Pinky Bautista, and Yukako Yagi. "Balancing Image Quality and Compression Factor for Special Stains Whole Slide Images." Analytical Cellular Pathology 35, no. 2 (2012): 101–6. http://dx.doi.org/10.1155/2012/960684.

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The objective is to find a practical balance between quality and performance for daily high volume whole slide imaging. We evaluated whole slide images created by various scanners at different compression factors to determine the best suitable quality factor (QF) needed for pathological images of special stains.Method: We scanned two sets of eight special stains slides each at 0.50 μm/pixel resolution in Hamamatsu scanner at six and fiveQFlevels respectively to generate 72 images which were observed at a calibrated monitor by imaging specialists, a histo-technician, and a pathologist to find t

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Antonio, Franz E., Jomhel Cabatac, Syverson D. Cubillo, and Gecelene C. Estorico. "EXTRACTION OF LEMON PEEL (Citrus limon L.) AS AN ORGANIC STAIN REMOVER FOR PARTICULATE AND OXIDIZABLE STAINS." GUILD OF EDUCATORS IN TESOL INTERNATIONAL RESEARCH JOURNAL 2, no. 4 (2024): 48–57. https://doi.org/10.5281/zenodo.14293133.

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Fabric plays a key role in modern society, used widely in fashion and home decor. Different fabrics, including synthetics like polyester, have unique properties that determine their applications. Stains, caused by foreign substances, are categorized into four types: enzymatic, oxidizable, greasy, and particulate. Stain removal often involves chemical-based products, which can be harmful. This study shows that Lemon Peel Extract (Citrus Limon L.) can remove oxidizable and particulate stains. With titrimetric and colorimetric analysis, it was found that 54.83% of particulate and 54.14% of oxidiz

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Smith, Ronald W., and Victoria Bryg. "Staining Polymers for Microscopical Examination." Rubber Chemistry and Technology 79, no. 3 (2006): 520–40. http://dx.doi.org/10.5254/1.3547949.

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Abstract Polymers find widespread use in blends, graft copolymers, polymer alloys, and composites. Staining is an important tool for microscopically visualizing polymer phases and morphologies in these multi-component systems. Three stains have been used in most investigations. These stains (and the functional groups they are aimed at) are: osmium tetroxide for chemical unsaturation; ruthenium tetroxide for styrenics; and phosphotungstic acid for polyamides. Beyond these three basic stains there are a significant number of additional ones that have been developed for special cases. Aside from

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Gurung, P., S. Hirachand, R. Thapa, et al. "Utility of Rapid Brilliant Cresyl Blue Stain in Routine Fine Needle Aspiration Cytology." Journal of Institute of Medicine Nepal 39, no. 1 (2017): 16–21. http://dx.doi.org/10.59779/jiomnepal.697.

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Introduction: Rapid on-site evaluation is commonly performed by pathologists to check for cellular adequacy of fine needle aspirate smears to reduce hospital visits of patients and to make preliminary diagnosis. For rapid evaluation, laboratories use Romanowsky stain, Hematoxylin and Eosin stain, supravital stains and ultra-fast Papanicolaou stain. The aim of the study is to evaluate fine needle aspirate smears on-site for sample adequacy using supravital stain Brilliant Cresyl Blue (BCB), to make preliminary cytological diagnosis and to compare with the routine cytological Papanicolaou stain

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Warburton, M. J., S. A. Ferns, C. M. Hughes, and P. S. Rudland. "Characterization of rat mammary cell types in primary culture: lectins and antisera to basement membrane and intermediate filament proteins as indicators of cellular heterogeneity." Journal of Cell Science 79, no. 1 (1985): 287–304. http://dx.doi.org/10.1242/jcs.79.1.287.

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Three morphologically distinct major cell types were observed in primary cultures obtained from the mammary parenchyma of glands from virgin rats. These cell types consisted of small cuboidal epithelial cells, larger epithelioid cells and elongated cells. We have investigated the distribution of the basement membrane proteins laminin and type IV collagen, and the intermediate filament proteins vimentin and prekeratin, in these three cell types using immunofluorescence techniques. Antisera to the basement membrane proteins stain the large epithelioid cells and the elongated cells, but do not st

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Ming, Jun Feng, Ren Huang Wang, Su Xiang Tang, and Hong Jiang Liu. "Approaches to the Feather Stain Detection and Segmentation." Advanced Materials Research 317-319 (August 2011): 931–36. http://dx.doi.org/10.4028/www.scientific.net/amr.317-319.931.

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Extraction of feather defects is difficult because of their complicated and diversified characteristics. It is proposed that feather stain defects be extracted in HSI color space according to color characters of different types of feather defects. The improved variational level set method is adopted to locate and segment the feather stains on that basis. Experiment results show that the proposed methods can extract feather stains effectively and segment them accurately.

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Alonso, Lise, Thomas Pommier, Danis Abrouk, et al. "Microbiome Analysis of New, Insidious Cave Wall Alterations in the Apse of Lascaux Cave." Microorganisms 10, no. 12 (2022): 2449. http://dx.doi.org/10.3390/microorganisms10122449.

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Lascaux Cave is a UNESCO site that was closed to the public following wall surface alterations. Most black stains that had formed on wall surface are stable or receding, but a new type of alteration visually quite different (termed dark zones) developed in Lascaux’s Apse room in the last 15 years. Here, we tested the hypothesis that dark zones displayed a different microbial community than black stains previously documented in the same room, using metabarcoding (MiSeq sequencing). Indeed, dark zones, black stains and neighboring unstained parts displayed distinct microbial communities. However

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Lee, K. A. Bunding, Jeanne O'Brien, C. L. Kuesten, John Sramek, Mary H. Luccas, and Bonnie Aldrich. "Comparison of Human Panels, Colorimeter and near Infrared Spectroscopy for the Evaluation of Fabric Stain Removal." Journal of Near Infrared Spectroscopy 2, no. 2 (1994): 101–18. http://dx.doi.org/10.1255/jnirs.37.

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Near infrared (NIR) spectroscopy was used to analyse fabric stain samples used in a fabric stain prespotter test in which a colorimeter and human panels were used to evaluate the cleaning ability of the prespotters. The purpose of this was to see if the NIR results compared well with the other two techniques and could then be used instead for fabric stain analysis. NIR/visible instruments offer several advantages including determination of coloured and uncoloured components of the stains, ease of comparing the stain before and after cleaning, fast, accurate, reproducible and quantitative analy

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Aritonang, Intan, and Devi Ray Syahfitri Sinulingga. "EFEKTIVITAS PEMBERIAN CITRUS BAKING SODATERHADAP PENGHILANGAN STAIN PADA PRIA PEROKOK USIA 20-55 TAHUN DI KELURAHAN TANJUNGBALAI KOTA II, Lk. III KECAMATAN TANJUNGBALAI SELATAN." Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) 14, no. 1 (2019): 15–22. http://dx.doi.org/10.36911/pannmed.v14i1.555.

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Citrus and baking soda are kitchen ingredients that are often found in everyday life, have ingredients thatcan blot stains on teeth, especially stains of cigarettes or stains that can affect the color of teeth. This type ofresearch is analytic research with a quasi experiment method which aims to determine the effectiveness ofgiving citrus with baking soda to stain removal in smokers aged 20-55 years Tanjungbalai Kota II Village,Lk. III, South Tanjungbalai District with a sample of 30 people. The results showed that the average stainstate before being given was given citrus gel with baking sod

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Ghritlahare, Himanta, A. Einstein, Swatantra Patel, Rashmi C. Kerketta, Shubhangi D. Mishra, and Shirish K. Kujur. "Cost-Effective Staining Alternatives to Immunohistochemistry for Keratin Demonstration in Oral Epithelial Pathologies." Journal of Pharmacy and Bioallied Sciences 16, Suppl 3 (2024): S2827—S2829. http://dx.doi.org/10.4103/jpbs.jpbs_313_24.

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ABSTRACT Background: Demonstrating keratin is crucial in diagnosing various epithelial pathologies and is typically done through histological and immunohistochemical (IHC) methods. While IHC staining for keratin is highly specific, it can be costly and time-consuming. Therefore, common histological staining methods for keratin, suitable for routine histotechniques labs, are gaining importance. Methodology: Twenty formalin-fixed, paraffin-embedded tissue blocks, each representing histologically confirmed normal oral mucosa (NOM), hyperorthokeratosis (HOK), and well-differentiated squamous cell

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Hitalia, Jurish Pauleen, Yca Justerine Bringuelo, Ivan Henry Jordan, et al. "Kamias (Averrhoa Bilimbi), Starfruit (Averrhoa Carambola), and Karamay (Phyllanthus Acidus) Fruit Extract as Alternative Stain Remover." ASEAN Journal of Science and Engineering 1, no. 1 (2021): 19–22. http://dx.doi.org/10.17509/ajse.v1i1.33684.

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This study generally aimed to determine the effectiveness of kamias, star fruit, and karamay fruit extracts in removing stains. Previous studies revealed the effectiveness of Kamias as a stain remover, while, this study compared the effectiveness of different fruit extracts as a stain remover and the potential utilization of other fruit extracts from trees that are locally available. We determined the cost of making stain remover using kamias, karamay, and star fruit extracts, the ability of these fruit extracts as a stain remover in terms of the degree of strain reduction and whitening of the

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Attaway, C. C., H. Van Huele, and D. Rhoads. "Identifying strengths and weaknesses at the bench, an institution’s quest to improve the Gram stain bench experience and patient care." American Journal of Clinical Pathology 162, Supplement_1 (2024): S104. http://dx.doi.org/10.1093/ajcp/aqae129.232.

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Abstract Introduction/Objective The Gram stain is the first actionable result reported to guide treatment. In our laboratory, we noted instances of initial Gram stain results discrepant to subsequent cultures. In reevaluating the Gram stain bench, we devised a survey for medical laboratory scientists (MLS) to determine the strengths and weaknesses of the current workflow to search for areas of improvement. Results of the survey will help both leadership and MLS approach improving the interpretation and reporting of Gram stains. Methods/Case Report A 16-question survey was issued to all MLS in

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Al-Khafaji, Ali Khalil I., Haider H. Jaleel Al-Shammari, and Salim R. Hamudi Al-Obeidi. "Bone Marrow Fibrosis in Chronic myeloid leukemia (CML) and other Myeloproliferative Disorders Evaluated by Using Special Histochemical Stains for Collagen." Journal of the Faculty of Medicine Baghdad 53, no. 3 (2011): 296–300. http://dx.doi.org/10.32007/jfacmedbagdad.533833.

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Background: It is still difficult to give a final diagnosis in chronic myeloproliferative disorders (CMPDs) because of the overlap of the common pathological and clinical features of these disorders like bone marrow fibrosis which is considered important because it affects the normal function of the bone marrow. The collagen fibers are of different types, but in the bone marrow, the two main types are: collagen I, which is the most abundant type and collagen III (reticular) which is often associated with type I.Objectives:To study bone marrow fibrosis (BMF) in samples of bone marrow biopsies (

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Hanker, J. S., and B. L. Giammara. "Silver stains for the light and electron microscopic demonstration of spirochete changes in syphilis and AIDS." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 764–65. http://dx.doi.org/10.1017/s0424820100161382.

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Two new silver stains have been developed in our laboratories to stain, and possibly identify without culture or immunocytochemical staining, Gram(-) bacteria. The first is the PATS reaction which positively stains Gram(-) bacteria (Figs. 1, 2) including those like spirochetes which are difficult to culture. Another stain for Gram(-), as well as Gram(+), bacteria is a variation of the Gram stain. Ordinarily to stain Gram(+) bacteria, and not Gram(-) bacteria, the crystal violet stain is removed from Gram(-) microbes by rinsing with alcohol/acetone. If this rinse step is omitted, the crystal vi

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Karadayi, Sukriye, Elnaz Moshfeghi, Tulin Arasoglu, and Beytullah Karadayi. "Evaluating the persistence of laundered semen stains on fabric using a forensic light source system, prostate-specific antigen Semiquant test and DNA recovery-profiling." Medicine, Science and the Law 60, no. 2 (2020): 122–30. http://dx.doi.org/10.1177/0025802419896935.

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Semen stains on the clothes of victims of sexual assault can remain as evidence even after garments have been laundered. In this study, we aimed to investigate the effectiveness of commonly preferred methods to detect semen stains in two different fabric types that were laundered with different washing machine programmes and washing powders, and to obtain a DNA profile from the semen stains. For this purpose, a comprehensive study was performed on semen-stained underwear using three different methods for stain detection, confirmation and identification: a forensic light source (FLS) system, th

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Childs, Gwen V., Geda Unabia, and Jonathan Lloyd. "Adapting cytochemical protocols to localize antigens, labeled-ligands, and labeled probes for mRNA." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 992–93. http://dx.doi.org/10.1017/s0424820100129243.

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Current methods available in immunocytochemistry can be adapted to obtain multiple stains for antigens, labeled-ligands, and/or mRNA and thus achieve a wider view of the structural and functional organization of a cell. In this presentation, applications of immunocytochemical stains for pituitary hormones in combination with affinity cytochemical stains for labelled probes for ligand-receptor complexes or messenger RNA will be described along with a practical view of how each stain is quantified.The ABC peroxidase stains are useful for studies of antigens in semi-thin (1 μm) plastic-embedded s

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