Log in

Relevant bibliographies by topics / Staphylococcal protein A (SpA) / Dissertations / Theses

To see the other types of publications on this topic, follow the link: Staphylococcal protein A (SpA).

Dissertations / Theses on the topic 'Staphylococcal protein A (SpA)'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 dissertations / theses for your research on the topic 'Staphylococcal protein A (SpA).'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.

1

Osei, Awuku Kwabena. "Superantigen-like interaction of IVIG with antibody Fab fragments cloned by phage display technology." Doctoral thesis, [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=96462785X.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Gao, Jinxin. "Regulatory elements in the Staphylococcus aureus protein A (Spa) gene promoter and characterization of SarS binding to the Spa promoter /." Search for this dissertation online, 2004. http://wwwlib.umi.com/cr/ksu/main.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Roberts, Jill Carolyne. "Characterization of Community-acquired Methicillin-resistant Staphylococcus aureus by Pulsed-field Gel Electrophoresis, Multilocus Sequence Typing, and Staphylococcal Protein A Sequencing: Establishing a Strain Typing Database." [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001489.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Sauerwald, Claudia. "Die subklinische Staphylococcus aureus-Mastitis - Sanierung eines hessischen Milcherzeugerbetriebes und epidemiologische Untersuchungen mittels Staphylokokken-Protein A (spa)-Typisierung." Doctoral thesis, Universitätsbibliothek Leipzig, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-127925.

Full text

Abstract:

In der vorliegenden Studie wurde die Sanierung einer durch S. aureus verursachten Eutergesundheitsstörung in einem hessischen Milchviehbestand mittels klassischer Sanierungsmaßnahmen über einen Zeitraum von 18 Monaten begleitet. Durch konsequente Einhaltung der Sanierungsmaßnahmen nach dem Fünf-Punkte-Plan und die räumliche Trennung der Herde in eine S. aureus-positive und -negative Gruppe sank die S. aureus-Prävalenz im Betrieb innerhalb von 15 Monaten von 30% auf <1%. Nach 18 Monaten waren erstmalig keine Neuinfektionen mehr mit S. aureus zu verzeichnen. Zusätzlich zu den im Abstand von dre

APA, Harvard, Vancouver, ISO, and other styles

5

Stephens, Alex J. "The development of rapid genotyping methods for methicillin-resistant Staphylococcus aureus." Thesis, Queensland University of Technology, 2008. https://eprints.qut.edu.au/20172/1/Alexander_Stephens_Thesis.pdf.

Full text

Abstract:

Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen that is endemic in hospitals all over the world. It has more recently emerged as a serious threat to the general public in the form of community-acquired MRSA. MRSA has been implicated in a wide variety of diseases, ranging from skin infections and food poisoning to more severe and potentially fatal conditions, including; endocarditis, septicaemia and necrotising pneumonia. Treatment of MRSA disease is complicated and can be unsuccessful due to the bacterium's remarkable ability to develop antibiotic resistance.

APA, Harvard, Vancouver, ISO, and other styles

6

Stephens, Alex J. "The development of rapid genotyping methods for methicillin-resistant Staphylococcus aureus." Queensland University of Technology, 2008. http://eprints.qut.edu.au/20172/.

Full text

Abstract:

Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen that is endemic in hospitals all over the world. It has more recently emerged as a serious threat to the general public in the form of community-acquired MRSA. MRSA has been implicated in a wide variety of diseases, ranging from skin infections and food poisoning to more severe and potentially fatal conditions, including; endocarditis, septicaemia and necrotising pneumonia. Treatment of MRSA disease is complicated and can be unsuccessful due to the bacterium's remarkable ability to develop antibiotic resistance.

APA, Harvard, Vancouver, ISO, and other styles

7

Sauerwald, Claudia [Verfasser], Axel [Akademischer Betreuer] Sobiraj, and Axel [Gutachter] Sobiraj. "Die subklinische Staphylococcus aureus-Mastitis - Sanierung eines hessischen Milcherzeugerbetriebes und epidemiologische Untersuchungen mittels Staphylokokken-Protein A (spa)-Typisierung / Claudia Sauerwald ; Gutachter: Axel Sobiraj ; Betreuer: Axel Sobiraj." Leipzig : Universitätsbibliothek Leipzig, 2013. http://d-nb.info/123852754X/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Taylor, M. J. "Protein engineering of staphylococcal protein A." Thesis, London School of Hygiene and Tropical Medicine (University of London), 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373965.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Löfblom, John. "Staphylococcal surface display for protein engineering and characterization." Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4584.

Full text

Abstract:

Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. Directed evolution has provided researchers with a tool for circumventing our limited knowledge and hence the possibility to create novel molecules that by no means could have been designed today. The approach is based on construction of high-complexity combinatorial librarie

APA, Harvard, Vancouver, ISO, and other styles

10

Löfblom, John. "Staphylococcal surface display for protein engineering and characterization /." Stockholm : Bioteknologi, Kungliga Tekniska högskolan, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4584.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Xu, Zhiqiang. "Molecular analysis of staphylococcal multidrug transport protein QacA." Thesis, The University of Sydney, 2005. https://hdl.handle.net/2123/27983.

Full text

Abstract:

The multidrug exporter QacA from Staphylococcus aureus confers resistance to a wide range of structurally-dissimilar monovalent and bivalent lipophilic, cationic compounds, including intercalating dyes, quaternary ammonium compounds (Qacs), diamidines, and biguanidines, many of which are used as antiseptics and disinfectants in current applications. To overcome such a problem, detailed understanding of the substrate-recognition and transport mechanisms of QacA is crucial. High-resolution structural studies of QacA can provide critical insights to these mechanisms. The preliminary requirement

APA, Harvard, Vancouver, ISO, and other styles

12

LOMBARDI, BENEDETTA. "Staphylococcal antigens characterization through a protein interaction approach." Doctoral thesis, Università del Piemonte Orientale, 2014. http://hdl.handle.net/11579/46757.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Wernérus, Henrik. "Engineering of staphylococcal surfaces for biotechnological applications." Doctoral thesis, KTH, Biotechnology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3450.

Full text

Abstract:

<p>The engineering of bacterial surfaces has in recent yearsattracted a lot of attention with applications in manydifferent areas of bioscience. Here we describe the use of twodifferent surface display systems for the gram-positivebacteria Staphylococcus carnosus and Staphylococcus xylosus invarious biotechnological applications.</p><p>Environmental microbiology currently attracts a lot ofattention since genetically engineered plants and bacteriamight be used as bioadsorbents for sequestration of toxicmetals. Bacterial surface display of metal-binding peptidesmight enable recycling of the biom

APA, Harvard, Vancouver, ISO, and other styles

14

Yarawsky, Alexander E. "Reversible assembly and amyloidogenesis of the staphylococcal biofilm protein, Aap." University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1560865959517373.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Kronqvist, Nina. "Staphylococcal surface display in directed evolution." Doctoral thesis, KTH, Molekylär Bioteknologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11555.

Full text

Abstract:

Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. In this approach, generally termed directedevolution, high-complexity combinatorial libraries are created from which affinity proteins areisolated using an appropriate selection method, thus cir

APA, Harvard, Vancouver, ISO, and other styles

16

Atkins, Karen. "Structural and functional studies of bacterial adhesion proteins : Staphylococcus aureus immunoglobulin-binding proteins Sbi and SpA and their interactions with serum proteins." Thesis, University of Bath, 2006. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432400.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Patel, Deepa. "Functional and structural characterisation of staphylococcal superantigen-like protein 10 (SSL10)." Thesis, University of Auckland, 2011. http://hdl.handle.net/2292/6733.

Full text

Abstract:

Staphylococcus aureus is a highly versatile gram-positive bacterium that owes its success to the remarkable range of pathogenic factors that it has at its disposal. Staphylococcal superantigen-like protein 10 (SSL10) is a tightly regulated, highly conserved protein exclusive to the arsenal of S.aureus. It is located on the genomic island vSa�� alongside ten other related ssl genes. The 2.75 �� crystal structure of SSL10 displays strong structural homology to the superantigen toxins with an N-terminal OB-fold domain linked to a C-terminal ��-grasp domain; however they are functionally distinct.

APA, Harvard, Vancouver, ISO, and other styles

18

Thomas, Christopher David. "Mechanistic studies of rep D, a staphylococcal plasmid replication initiator protein." Thesis, University of Leicester, 1988. http://hdl.handle.net/2381/35169.

Full text

Abstract:

The DNA sequences of many small staphylococcal plasmids possess open reading frames with 70-80 sequence identity to that of the repD product of the chloramphenico1-resistance plasmid pC221. This thesis describes the results of studies of the REP D protein, required for initiation of replication of pC221 both in vivo and in vitro. REP D was expressed in Escherichia coli, using a purpose-built expression vector, as 10-15% of the total cellular protein. Purification yields 40-50mg of REP D from one litre of induced culture. The protein displays a molecular weight of 35 kDa on denaturing gel elect

APA, Harvard, Vancouver, ISO, and other styles

19

Tsukamoto, Noriyuki. "Rap1 GTPase-activating Protein SPA-1 Negatively Regulate Cell Adhesion." Kyoto University, 1999. http://hdl.handle.net/2433/181245.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Bottomley, Stephen Paul. "The site-directed mutagenesis of an IgG-binding protein based upon protein A from Staphylococcal aureus." Thesis, University of Southampton, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295705.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Chaton, Catherine T. "Metal Binding Specificity and N-terminal Function of the Staphylococcal Biofilm Protein Aap." University of Cincinnati / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin151092604272917.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Plummer, Ben Thomas. "The influence of pH on the binding of immunoglobulin G to staphylococcal protein A." Thesis, University of Canterbury. Chemical and Process Engineering, 2013. http://hdl.handle.net/10092/7952.

Full text

Abstract:

The interaction between protein A and immunoglobulin G (IgG) was studied at a variety of pH values using a surface plasmon resonance (SPR) device, which provides real time kinetic data without labelling or molecular alteration. This study was carried out due to the large scale use of Protein A affinity chromatography for the purification of IgG for pharmaceutical purposes, and is one of the most costly steps in the purification process. The results produced were largely in line with those produced in previous literature with binding remaining strong between pH 7.4 and 5.0, although the associa

APA, Harvard, Vancouver, ISO, and other styles

23

Sade, Robert Silas. "Spectroscopic studies on a model denatured state of the B domain of staphylococcal protein A." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608677.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

James, Benjamin Arthur Frederick. "Chemical synthesis of Staphylococcal protein A IgG-binding domain peptides and their structural and biological characterisation." Thesis, Open University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335198.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Miles, Jr George Emmett. "On the structure and assembly of staphylococcal leukocidin: a study of the molecular architecture of beta-barrel pore-forming toxins." Texas A&M University, 2003. http://hdl.handle.net/1969.1/3952.

Full text

Abstract:

Staphylococcal leukocidin pores are formed by the obligatory interaction of two distinct polypeptides, one of class F and one of class S, making them unique in the family of β-barrel pore-forming toxins (β-PFTs). By contrast, other β-PFTs form homooligomeric pores. For example, the staphylococcal α- hemolysin is a homoheptamer. Limited and controversial data exist on the assembly and molecular architecture of the leukocidin pore. In this work, biochemical and biophysical methods were used to characterize the leukocidin pore produced by the LukF (HlgB) and LukS (HlgC) compon

APA, Harvard, Vancouver, ISO, and other styles

26

Hjelm, Barbara. "Epitope mapping of antibodies towards human protein targets." Doctoral thesis, KTH, Proteomik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-59529.

Full text

Abstract:

This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. Techniques for epitope mapping in a streamlined manner are therefore needed, particularly in high throughput projects as the Human Protein Atlas that aims to systematically generate two antibodies with se

APA, Harvard, Vancouver, ISO, and other styles

27

Harazaki, Masashi. "Specific Recruitment of SPA-1 to the Immunological Synapse : Involvement of Actin-bundling Protein Actinin." Kyoto University, 2004. http://hdl.handle.net/2433/147566.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Thompson, James Russell. "Imaging the assembly of the Staphylococcal pore-forming toxin alpha-Hemolysin." Thesis, University of Oxford, 2009. http://ora.ox.ac.uk/objects/uuid:e320004a-6118-4dac-af2a-eca6e90be7ac.

Full text

Abstract:

Alpha-hemolysin is a pore-forming toxin secreted by pathogenic Staphylococcus aureus. Its spontaneous oligomerization and assembly into a trans-bilayer beta-barrel pore is a model for the assembly of many other pore-forming toxins. It is studied here in vitro as a means to probe general membrane protein oligomerization and lipid bilayer insertion. This thesis details the results of experiments to develop and implement a novel in vitro lipid bilayer system, Droplet-on-Hydrogel Bilayers (DHBs) for the single-molecule imaging of alpha-hemolysin assembly. Chapter 2 describes the development of DHB

APA, Harvard, Vancouver, ISO, and other styles

29

Shannon, Oonagh. "Biological effects of extracellular fibrinogen binding protein (Efb) in Staphylococcus aureus infection /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-275-6/.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Scietti, Luigi Angelo Domenico <1986&gt. "Exploring host-pathogen interactions through protein microarray. Large-scale protein microarray analysis revealed novel human receptors for the staphylococcal immune evasion protein FLIPr and for the neisserial adhesin NadA." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amsdottorato.unibo.it/6994/1/Luigi_Scietti_PhD_thesis_final.pdf.

Full text

Abstract:

Adhesion, immune evasion and invasion are key determinants during bacterial pathogenesis. Pathogenic bacteria possess a wide variety of surface exposed and secreted proteins which allow them to adhere to tissues, escape the immune system and spread throughout the human body. Therefore, extensive contacts between the human and the bacterial extracellular proteomes take place at the host-pathogen interface at the protein level. Recent researches emphasized the importance of a global and deeper understanding of the molecular mechanisms which underlie bacterial immune evasion and pathogenesis. Thr

APA, Harvard, Vancouver, ISO, and other styles

31

Scietti, Luigi Angelo Domenico <1986&gt. "Exploring host-pathogen interactions through protein microarray. Large-scale protein microarray analysis revealed novel human receptors for the staphylococcal immune evasion protein FLIPr and for the neisserial adhesin NadA." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amsdottorato.unibo.it/6994/.

Full text

Abstract:

Adhesion, immune evasion and invasion are key determinants during bacterial pathogenesis. Pathogenic bacteria possess a wide variety of surface exposed and secreted proteins which allow them to adhere to tissues, escape the immune system and spread throughout the human body. Therefore, extensive contacts between the human and the bacterial extracellular proteomes take place at the host-pathogen interface at the protein level. Recent researches emphasized the importance of a global and deeper understanding of the molecular mechanisms which underlie bacterial immune evasion and pathogenesis. Thr

APA, Harvard, Vancouver, ISO, and other styles

32

Xie, Tian. "Scintillation proximity assay (SPA) measuring p53 DNA binding and total p53 level in human thyroid cancer cell line ARO." Diss., Online access via UMI:, 2007.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

33

Adie, Jillian E. "Structure-based drug design of 11β-hydroxysteroid dehydrogenase type 1 inhibitors". Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4673.

Full text

Abstract:

The enzyme 11β-Hydroxysteroid Dehydrogenase 1 (11β-HSD1) catalyses the intracellular biosynthesis of the active glucocorticoid cortisol. Tissue specific dysregulation of the enzyme has been implicated in the development of metabolic syndrome and other associated diseases. Experiments with transgenic mice and prototype inhibitors show that inhibition of 11β-HSD1 in visceral adipose tissue and liver leads to a resistance of diet-induced hyperglycemia and a favourable lipid and lipoprotein profile as compared to controls. 11β-HSD1 inhibition has thus been proposed as an effective strategy to decr

APA, Harvard, Vancouver, ISO, and other styles

34

Mandelli, Andrea Paola. "Unfolding the immune response against Staphylococcus aureus-mediated systemic sequelae of skin recurrences." Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1203731.

Full text

Abstract:

Staphylococcal protein A (SpA) is a surface-associated virulence factor of Staphylococcus aureus (S. aureus) which binds human immunoglobulins via both Fc and Fab fragment masking the pathogen to the host immune system. This activity interacts with the normal maturation of the host immune system during an infection and allows S. aureus to cause recurrent infections as, for example, skin recurrences. Skin recurrences are not only bothersome superficial infections that require continuous treatments, but may also evolve in more complicated and systemic complications. Immunization with SpA protec

APA, Harvard, Vancouver, ISO, and other styles

35

Chen, Jialong, Quanli Li, Jianguang Xu, Le Zhang, Manfred F. Maitz, and Jun Li. "Thromboresistant and rapid-endothelialization effects of dopamine and staphylococcal protein A mediated anti-CD34 coating on 316L stainless steel for cardiovascular devices." Royal Society of Chemistry, 2015. https://tud.qucosa.de/id/qucosa%3A36067.

Full text

Abstract:

There is convincing evidence in vivo that the vascular homing of endothelial progenitor cells (EPCs) contributes to rapid endothelial regeneration, which could prevent thrombosis and restenosis of cardiovascular devices. To enhance the EPC homing on cardiovascular devices, immobilization of an EPC capture agent (e.g. an anti-CD34 antibody) on the surface of cardiovascular devices is critical. We describe a way of immobilizing anti-CD34 Ab on 316L Stainless Steel (316L SS). For this, surface modification of 316L SS was performed via self-polymerization of dopamine (DA) and covalent grafting of

APA, Harvard, Vancouver, ISO, and other styles

36

Jariwala, Nidhi H. "Characterization of Staphylococcal nuclease and tudor domain containing protein 1 (SND1) as a molecular target in Hepatocellular carcinoma and Non-alcoholic steatohepatitis." VCU Scholars Compass, 2017. https://scholarscompass.vcu.edu/etd/5183.

Full text

Abstract:

CHARACTERIZATION OF STAPHYLOCOCCAL NUCLEASE AND TUDOR DOMAIN CONTAINING PROTEIN 1 (SND1) AS A MOLECULAR TARGET IN HEPATOCELLULAR CARCINOMA AND NON-ALCOHOLIC STEATOHEPATITIS Nidhi Jariwala, PhD A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Integrative Life Sciences Virginia Commonwealth University, 2017 Devanand Sarkar, M.B.B.S., PhD. Associate Professor, Department of Human and Molecular Genetics Virginia Commonwealth University Richmond, Virginia SND1, a subunit of the miRNA regulatory complex RISC, has been implicated as an onco

APA, Harvard, Vancouver, ISO, and other styles

37

Alm, Tove. "Interaction engineered three-helix bundle domains for protein recovery and detection." Doctoral thesis, KTH, Proteomik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-12823.

Full text

Abstract:

HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. Traditionally, monoclonal antibodies have been the natural choice for biotechnological and therapeutic applications due to their ability to bind a huge range of different molecules and their natural good affinity. However, the large size of antibodies (15

APA, Harvard, Vancouver, ISO, and other styles

38

Fleetwood, Filippa. "Bacterial display systems for engineering of affinity proteins." Doctoral thesis, KTH, Proteinteknologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-156420.

Full text

Abstract:

Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. An attractive technology for this purpose is cell surface display, offering many advantages, such as the quantitative isolation of high-affinity library members using flow-cytometric cell sorting. This thesis describes the development, evaluation and use of bacterial display technologies for the engineering of affinity proteins. Affinity proteins

APA, Harvard, Vancouver, ISO, and other styles

39

Åstrand, Mikael. "Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications." Doctoral thesis, KTH, Proteinteknologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-186279.

Full text

Abstract:

Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. In this thesis, the albumin-binding domain is explored as a scaffold for engineering novel affinity proteins with the possible benefit of combining a prolonged serum half-life with specific targeting in a single small scaffold protein. Previously, a library was created by randomizing surf

APA, Harvard, Vancouver, ISO, and other styles

40

Gülich, Susanne. "Engineering Proteinaceous Ligands for Improved Performance in Affinity Chromatography Applications." Doctoral thesis, KTH, Biotechnology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3327.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Röhrbein, Jan Hendrik [Verfasser]. "The influence of the staphylococcal Extracellular Adherence Protein on T cells functions in vitro in the context of the psoriasiform skin disease / Jan Hendrik Röhrbein." Ulm : Universität Ulm. Medizinische Fakultät, 2013. http://d-nb.info/1037395018/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Lindberg, Hanna. "Engineering of Affibody molecules targeting the Alzheimer’s-related amyloid β peptide". Doctoral thesis, KTH, Proteinteknologi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-173864.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Nilvebrant, Johan. "An albumin-binding domain as a scaffold for bispecific affinity proteins." Doctoral thesis, KTH, Proteomik, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-105425.

Full text

Abstract:

Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. In nature, antibodies are the primary affinity proteins and their usefulness has led to a widespread use both in basic and applied research. By means of combinatorial protein engineering and protein library technology, smaller antibody fragments or alternative non-immunoglobulin protein scaffolds can be engineered for various functions based on molecular recognition. In this thesis, a 46 amino acid small albumin-binding domain derived from streptococcal protein G was evaluated as a scaffold fo

APA, Harvard, Vancouver, ISO, and other styles

44

Hu, Francis Jingxin. "Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization." Doctoral thesis, KTH, Proteomik och nanobioteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-205410.

Full text

Abstract:

Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. Moreover, the growing interest in precision medicine requires a well-characterized target and antibody to predict the responsiveness of a treatment. This thesis describes the use of epitope information and display technologies to generate and characterize antibodies. In Paper I, we evaluated if the epitope information of a well-characterized pAb could be

APA, Harvard, Vancouver, ISO, and other styles

45

Ek, Moira. "Bacterial Display of a Tau-Binding Affibody Construct:Towards Affinity Maturation." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278580.

Full text

Abstract:

Aggregation of microtubule-associated protein tau is involved in the pathology of several neurodegenerative diseases, including Alzheimer’s disease. The affibody TP4 has been shown to inhibit this aggregation process, and its target-binding positions were previously grafted onto a dimeric affibody scaffold, creating the sequestrin seqTP4. This project constitutes a part of the affinity maturation process of seqTP4, using two different bacterial display methods. An error-prone PCR library was first expressed on Staphylococcus carnosus cells for selection of variants with improved tau-binding pr

APA, Harvard, Vancouver, ISO, and other styles

46

McGregor, Neil Roland. "An investigation of the association between toxin producing staphylococcus, biochemical changes and jaw muscle pain." University of Sydney. Prosthetic Dentistry, 2000. http://hdl.handle.net/2123/369.

Full text

Abstract:

Objectives: To assess the expression of the symptoms of jaw muscle pain and its association with alterations in biochemistry, other symptoms and the carriage of staphylococci. Methods: Three different study populations were assessed. The first was selected and examined by the author and consisted of 43 pain and 41 age and sex matched controls. The second was a study of CFS patients who were blinded to the author and the author subsequently examined the associations between jaw muscle symptom reporting and the standardised biochemistry measures. The third study was also blinded to the autho

APA, Harvard, Vancouver, ISO, and other styles

47

McGregor, Neil Roland. "An investigation of the association between toxin producing staphylococcus, biochemical changes and jaw muscle pain." Thesis, The University of Sydney, 1999. http://hdl.handle.net/2123/369.

Full text

Abstract:

Objectives: To assess the expression of the symptoms of jaw muscle pain and its association with alterations in biochemistry, other symptoms and the carriage of staphylococci. Methods: Three different study populations were assessed. The first was selected and examined by the author and consisted of 43 pain and 41 age and sex matched controls. The second was a study of CFS patients who were blinded to the author and the author subsequently examined the associations between jaw muscle symptom reporting and the standardised biochemistry measures. The third study was also blinded to the autho

APA, Harvard, Vancouver, ISO, and other styles

48

LING, DING-BANG, and 林定邦. "Application of staphylococcal protein A in diagnosis of toxoplasmosis." Thesis, 1987. http://ndltd.ncl.edu.tw/handle/67535815448704373339.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Chung, Matthew C. "Structural and functional characterisation of staphylococcal superantigen-like protein 11 (SSL11)." 2008. http://hdl.handle.net/2292/2613.

Full text

Abstract:

Staphylococcus aureus is a human pathogen with significant socioeconomic impact. It is the causative agent of many life-threatening disease states, including sepsis, staphylococcal toxic shock syndrome and necrotizing pneumonia. S. aureus is associated with 1% of all hospital stays and estimated costs of US$9.5 billion / year (for 2000 and 2001) in the USA alone (Noskin et al., 2005). Recently, a novel family of virulence factors produced by S. aureus was identified, which share sequence and structural homology to the infamous superantigens (Arcus et al., 2002; Williams et al., 2000) – protein

APA, Harvard, Vancouver, ISO, and other styles

50

Meinhold, Lars [Verfasser]. "Crystalline protein dynamics : a simulation analysis of staphylococcal nuclease / presented by Lars Meinhold." 2005. http://d-nb.info/977037444/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!