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Dissertations / Theses on the topic 'Stenol'
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1
Joffrion, Tiffany Michelle. "Sterol biosynthesis and sterol uptake in the fungal pathogen Pneumocystis carinii." University of Cincinnati / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1267556286.
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Almeida, de Carvalho Maria Joao. "Sterol requirements in Drosophila melanogaster." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-24817.
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Sterol is an abundant component of eukaryotic cell membranes and is thought to influence membrane properties such as permeability, fluidity and microdomain formation. Drosophila is an excellent model system in which to study functional requirements for membrane sterol because, although it does not synthesize sterol, it nevertheless requires sterols to complete development. Moreover, Drosophila normally incorporates sterols into cell membranes. Thus, dietary sterol depletion can be used to specifically reduce membrane sterol levels. In contrast, vertebrates do synthesize cholesterol. In this way, sterol depletion in vertebrates demand the use of approaches such as chemical extractions, drug treatments or genetic manipulation which are prone to have side effects. We have controlled the level and type of dietary sterol available to developing Drosophila larvae in order to investigate the requirement for sterol in cell membranes, and to distinguish it from the function of sterol as a precursor for signaling molecules. Strikingly, we show that membrane sterol levels can be reduced 6-fold in most tissues without affecting cell or larval viability. Larvae respond to sterol depletion by arresting their growth and development, and by increasing the level of specific sphingolipid variants that promote survival when sterol is scarce. Thus, non-sterol lipids are able to substitute for sterols in the maintenance of basic membrane biophysical properties required for life. Despite this, Drosophila larvae regulate their growth to maintain membrane sterol levels within tight limits. The existence of this novel membrane sterol-dependent growth control mechanism indicates an important role for bulk membrane sterol in the tissue specific functions of differentiated cells.
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Bahr, Sara von. "Studies on sterol 27-hydroxylase (CYP27A1) /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-824-6/.
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Yates, Phillip John. "Sterol biosynthesis and plant culture growth." Thesis, University of Liverpool, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317319.
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Othman, Rgia Ali. "Assessment of sterol metabolism in sitosterolemia." Taylor & Francis, 2012. http://hdl.handle.net/1993/24317.
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Sitosterolemia (STSL) is a sterol storage disorder characterized by very high plasma plant sterol (PS) and 5α-stanol levels, and leads to premature atherosclerosis, xanthomas, macrothrombocytopenia and endocrine disruption. Ezetimibe (EZE), a sterol absorption inhibitor, reduces plasma PS levels in STSL but its effect on tissue pool of sterols has not been investigated yet. The research objectives were to assess if EZE reduces whole body sitosterol and cholesterol pool sizes, improves cholesterol homeostasis, enhance hematologic profile and reduce endocrine disruption in STSL. EZE effects on circulating levels of cholestanol and its precursors (cholesterol and bile acid derivative 7α-hydroxy-4-cholesten-3-one, 7α-H-C4) relative to exogenous stanols (sitostanol) were also studied.
Eight STSL patients were taken off EZE for 14 wks. After 4 wks off EZE they received intravenous doses of D7-sitosterol and 18O-cholesterol for sterol pool sizes assessments, and oral doses of 13C-cholesterol and deuterium oxide to measure fractional cholesterol absorption and synthesis rates. EZE (10 mg/d) was resumed and stable isotopes testing repeated. Measurement parameters included isotopic sterol enrichments, blood cell count, plasma and red blood cell (RBC) PS, cholesterol and its precursor (lathosterol), 5α-stanols and plasma 7α-H-C4, and thyroid hormones levels. EZE reduced plasma levels of sitosterol and total cholesterol, whole body sitosterol and cholesterol pool sizes and fractional cholesterol absorption rate while increasing cholesterol synthesis, production and clearance rates. EZE increased platelet count and decreased platelet size without affecting RBC indices of size or mass. A substantial decrease in circulating sitostanol but moderate decrease of cholestanol was noted with EZE. EZE increased lathosterol but not 7α-H-C4, suggesting increases in cholesterol biosynthesis and thus precursor availability for synthesis of cholestanol. In summary, EZE reduces body stores of PS and cholesterol, and increases cholesterol turnover by reducing cholesterol absorption and enhancing its synthesis and clearance. EZE reduces circulating PS and 5α-stanol levels, and improves macrothrombocytopenia and thyroid disruption. Endogenous cholestanol in STSL is mainly derived from cholesterol but not bile acid synthesis pathway. These data suggest that EZE may reduce the risks of developing premature atherosclerosis, bleeding and hormone disruption, thereby reinforcing the rationale for the use of EZE in treatment of STSL.<br>February 2015
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Bavikar, S. N. "Design and synthesis of Sterol-Polyamides, Sterol-Polyamines and bile acid bistriazoles: a new class of antimicrobials." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 2009. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2766.
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Tabata, Rena Christina. "Neuropathology induced by sterol glucosides in mice." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/24178.
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Sterol glucosides are a family of compounds characterized by a carbohydrate unit bound to a tetracyclic carbon chain. They are found in high concentrations in cycad seeds which have been linked to the etiology of amyotrophic lateral
scierosis-parkinsonism dementia complex (ALS-PDC). The neurotoxicities of the three main cycad sterol glucosides, campesterol β-D-glucoside, stigmasterol β-Dglucoside (SG), and β-sitosterol β-D-glucoside (BSSG) have been demonstrated
previously in vitro. In the present study, we demonstrate that SC and BSSG exert neurotoxic effects in vivo. An outbred strain of mice was fed BSSG or SG (1000 μg daily) for a period of 15 wk and sacrificed immediately after or at 17 wk
later. A battery of behavioural tests, including rotarod, wirehang, modified leg extension reflex test, treadmill, and open field were used to monitor behavioural disturbances. An array of histological measures was also conducted to assess
the cellular impact of BSSG and SG. Behavioural test performance scores revealed that BSSG and SG impaired spinal reflexes and decreased overall
movement. In addition to this, SC was found to impair motor coordination and strength. Also, ventral plane videography of performance on a treadmill and open field tests indicated that SG-exposed animals were more anxious and
tended to drag and shuffle their limbs during forward movement. The cellular impact of dietary BSSG and SG exposures were also different. Animals exposed to each of the sterol glucosides showed an upregulation of activating transcription factor-3 and an increase in the incidence of phosphorylation of junser⁷³ in response to stress. However, BSSG-exposure induced neurodegeneration that primarily targeted large motor neurons whereas SC impacted a more diverse motor neuron population, including large and small motor neurons. Exposure to each of the sterol glucoside caused intense proliferation of astrocytes and microglia as well as a depletion of dopamine levels
in the substantia nigra and striatum. Lastly, SG-exposure induced the pathological aggregation of either tau or transactivating DNA binding protein-43 in some animals. The insights gained from this study will be useful for elucidating
the pathogenesis of ALS-PDC and related disorders.
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Malik, Vatsala. "Functional analysis of UDP-sugar : sterol glucosyltransferases." Thesis, Northumbria University, 2011. http://nrl.northumbria.ac.uk/7259/.
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Glycosyltransferases (GTs) are essential for the biosynthesis and diversification of many therapeutically important natural products. Of these, UDP-sugar: sterol glucosyltransferases (UGTs) (2.4.1.173) catalyse the synthesis of therapeutically important steryl glycosides (SGs). Guided by the sequence similarity with a previously characterised N-terminally truncated UGT from Saccharomyces cerevisiae (UGT51), this study reports the cloning of the gene fragment encoding the C-terminal catalytic domains from related yeasts and the expression and characterisation of their encoded products produced. N-terminally histidine tagged proteins were purified for in vitro assays against a panel of sterol and steroidal acceptors. Liquid chromatography-mass spectrometry (LC-MS) and kinetic analysis led to the successful characterisation of two novel UGTs from Pichia angusta and Kluyveromyces lactis. In addition, testosterone was shown to be utilized by all UGTs, including the previously characterised S. cerevisiae UGT51. Random mutagenesis of UGTs and homology modelling of the S. cerevisiae UGT revealed structural similarities with family 1 bacterial glycopeptide GTs. Given the structural and mechanistic similarities among GT family 1 UGTs, this approach may provide a template for genetic manipulation of novel UGTs from other members of the GT superfamily with a better understanding of catalytic domains and for broadening their scope in drug development. It may also aid the development of a generic process in the synthesis of SGs.
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Pullen, Margaret Leighton. "Studies on sterol biosynthesis mutants of Arabidopsis." Thesis, Durham University, 2005. http://etheses.dur.ac.uk/2952/.
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This thesis examines the gene promoter activity and morphological characteristics of mutants of HYDRA 1 (HYD 1) and HYDRA2 I FACKEL (HYD2 I FK) (Mayer et al. 1991, Topping et al. 1997) from Arabidopsis. These loci are unique, and encode components of the sterol biosynthesis pathway (Schrick et al. 2000, Souter et at. 2002). Various patterning processes are disrupted in hydra mutants (Topping et at. 1997), and bulk sterol profiles are altered (Schrick et at. 2000, Souter et al. 2002). The mutants show heightened responses to auxin, and their phenotype is partly ameliorated by inhibition of ethylene signalling (Souter et al. 2002, 2004, He et al. 2003). Although much previous attention has been given to the analysis of their phenotype, the precise basis of the pleiotropic defects seen in the hydra mutants have not been attributed to any single phenomenon.This thesis examines the hydra mutants' body patterning and morphology, and aims to test the hypothesis that hydra mutants are defective in pattern coordination across the radial axis. The basis of phenotypic rescue through reduced ethylene perception, as conveyed by the ethylene insensitive2 (ein2) mutation (Alonso et al. 1999), is also examined using anatomical and transgenic markers of pattern definition and phytohormone signalling response. Mutants at the hydra loci have a substantial inter-sibling variability, including duplication or dissociation of the longitudinal axis. Reporter activities of the HYDRA 1 (HYD1) promoter implies an association of gene activity with stipules, and functional epidermal cells and ground tissue in both root and shoot tissues at the point of cell differentiation. Reporter expression defines a radial gradient across the root longitudinal axis which is maximal in thedifferentiation zone. All cell types highlighted by pHYD1 GUS activity sow anomalous cellular patterning in the root and rosette of hydra mutant seedlings, although pattern definition in lateral organs of the inflorescence stem appear relatively normal. Tissues of the hydra embryo and vegetative rosette have ectopic cell division activity; this persists in cotyledons beyond the point where wild-type cotyledon development has ceased Reduced ethylene perception via ein2 appears to confer a partial rescue of the hydra phenotype by facilitating an earlier transition from cell division to cell fate commitment, thus allowing greater coordination between cells in longitudinal cell files. This phenomenon may be attributable to enhanced auxin transport. In contrast, shoot dorsiventral cues are variably skewed or reversed, correlating with a loss of stipular function, in a manner independent of ethylene signalling. Other phytohormone signalling systems, as revealed by reporter constructs for auxin, cytokinin and gibberellin responsive genes, show a varied activity between seedlings. All of these responses appear anomalous in hydra single mutants, some with distinct differences between the two mutant sibling populations. These responses are partly modulated by ein2 in the hydra-ein2 double mutants, although ein2 itself has little or no effect on reporter activity. In particular the distinctive differences in cytokinin positional response between the two hydra mutants are abolished by the presence of ein2. HYDRA gene activity appears to modulate radial patterning and differentiation associated processes. The mutant shoot phenotypes suggest a role for sterolsin the definition of organ lateral boundaries and coordinated centrolateral expansion in flattened organs. In the mutant root, the control of the transition from division to differentiation in cortex cells is disrupted in hydra and may reflect a disrupted phosphate perception. As HYDRA gene activity is associated specifically with functional cells in the epidermis, this suggests that sterols may activate a mechanism for the timed differentiation of 'target cells'. Models are proposed to integrate the HYDRA gene expression data and the hydra mutant phenotype into a functional scheme of plant development.
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Williams, Steven Geraint. "Factors influencing sterol metabolism in brewing yeasts." Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.253296.
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Sullivan, David Patrick. "Intracellular sterol transport and distribution in saccharomyces cerevisiae /." Access full-text from WCMC, 2009. http://proquest.umi.com/pqdweb?did=1692359491&sid=3&Fmt=2&clientId=8424&RQT=309&VName=PQD.
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Hascalovici, Jacob. "Heme Oxygenase-1/sterol interactions in Alzheimer's disease." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119485.
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As average life expectancy increases, understanding the pathogenesis of age related degenerative brain disease such as Alzheimer's is of paramount importance. The brain is the most cholesterol dense human organ and normal neurophysiology is highly dependant upon this sterol. Sterol dys-regulation has been widely implicated in the pathogenesis of neurodegeneration, yet investigative efforts have failed to determine how these essential disturbances drive disease progression. The heme oxygenases (HOs) are dynamic sensors of cellular oxidative stress and likely mediators of tissue redox homeostasis across the phylogenetic spectrum. However, the heme catabolic process has proven to be one of janus face! Up-regulation of heme oxygenase-1 and altered cholesterol metabolism are characteristic of Alzheimer-diseased (AD) neural tissues. Our lab has previously demonstrated the propensity for HO-1 overexpression to impact sterol homeostasis. Herein, we query the mechanisms behind these HO-1/sterol interactions in vitro (cultured rat astroglia) and examine this relationship in post-mortem human AD brain (Religious Orders Study) and in vivo by comparing two mouse models; 1) a novel HO-1 transgenic mouse (GFAP.HMOX1) engineered to selectively express human HO-1 in the astrocytic compartment and 2) the previously described triple transgenic AD mouse (3xTg-AD). Data garnered from our experimentation indicate that HO-1 is a profound effecter of sterol regulation in the central nervous system and suggests that, under various conditions of cellular stress, HO-1 differentially impacts patterns of brain sterols and oxysterols. We present a novel model of brain sterol regulation in AD across the pathological spectrum and delineate the importance of HO-1 in this process. Aberrancy of HO-1/sterol dynamics akin to those reviewed herein constitute a ubiquitous pathway of pathological aging and, hence, a robust target for therapeutic intervention in AD and similar degenerative diseases.<br>Comprendre la pathogenèse d'une maladie dégénérative du cerveau telle la maladie d'Alzheimer est d'une importance primordiale pour notre population vieillissante. Le cerveau est l'organe du corps humain avec le plus haut taux de cholestérol, et la neurophysiologie normale dépend fortement de ce stérol. La dysrégulation du stérol a été largement impliquée dans la pathogenèse de la neurodégénérescence, mais les efforts de recherche n'ont pas réussi à déterminer comment ces perturbations nécessaires causent la maladie. Les oxygénases hème sont des capteurs dynamiques de stress oxydatif cellulaire et des médiateurs probables de l'homéostasie redox tissulaire à travers le spectre phylogénétique. Toutefois, le processus catabolique hème s'est avéré être l'un des visages de janus! La surexpression de l'hème oxygénase-1 et le métabolisme du cholestérol altéré est caractéristique des tissues neurologiques affectés par la maladie d'Alzheimer. Notre laboratoire a précédemment démontré que la propension de HO-1 surexpression peut avoir un impacte sur l'homéostasie des stérols. Dans les présentes, nous enquêtons les mécanismes derrière ces interactions HO-1/sterol in vitro (astrocytes de rat en culture) et examinons cette relation dans le cerveau atteint d'Alzheimer post-mortem (Religious Order Study) et in vivo en comparant deux modèles de souris; 1) une souris HO-1 transgénique (GFAP.HMOX1) conçue pour faire ressortir de façon sélective de la HO-1 humain dans le compartiment astrocytic et 2) une souris transgénique triple d'Alzheimer (3xTg-AD), décrite précédemment. Les données résultantes de notre expérimentation indiquent que la HO-1 est un profond effecteur de la régulation des stérols dans le système nerveux central et suggère que, dans diverses conditions de stress cellulaire, la HO-1 impact de façon différentielle les modes des stérols et oxystérols du cerveau. Nous présentons un nouveau modèle de régulation neurosterol d'Alzheimer's à travers le spectre pathologique et délimitons l'importance de la HO-1 dans ce processus. Mauvais dynamiques HO-1/stérol semblables à celles examinées dans les présentes constitue une voie omniprésente du vieillissement pathologique et, par conséquent, une cible solide pour l'intervention thérapeutique de la maladie d'Alzheimer et de maladies dégénératives similaires.
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Malmgren, Peter, and Pontus Steneros. "Stenen på Stadion." Thesis, Linnéuniversitetet, Institutionen för medier och journalistik (MJ), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-23848.
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Chan, Yen-Ming 1980. "The effect of fatty acid composition of plant sterol esters on blood lipid profiles and plasma plant sterol levels in hypercholesterolemic subjects /." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97923.
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To evaluate the relative efficacy of plant sterols (PS) esterified with the fatty acids from fish oil (PS-FO), olive oil (PS-OO) and sunflower oil (PS-SO) on blood lipid and PS concentrations, 21 hyperlipidemic subjects were randomly assigned to each of five treatments for 28 days using a cross-over design. The results showed that: (1) in a comparison of olive oil (OO), fish oil (FO), PS-FO and PS-SO subgroup, PS-FO reduced triacylglycerols (TG) relative to PS-SO. Total cholesterol (T-C)/high-density lipoprotein cholesterol (HDL-C) ratio was reduced with PS-FO compared with FO. Plasma PS levels were increased with PS-SO and PS-FO. (2) in a comparison of OO, PS-SO and PS-OO subgroup, PS-OO had a larger decrease in T-C than OO, while PS-SO and OO reduced T-C equally. Both PS-SO and PS-OO elevated plasma PS levels. Overall, PS-FO and PS-OO have a higher potential for decreasing the risk of cardiovascular disease in hyperlipidemic subjects than PS-SO and OO supplementations.
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Santos, Mariana De Fátima Silva. "Dissecting the intricacies of sterol dysregulation in Huntington's disease." Thesis, University of Leicester, 2016. http://hdl.handle.net/2381/38095.
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Background: Huntington’s disease (HD) is a devastating neurodegenerative condition, caused by an expanded polyglutamine tract in the Huntingtin (Htt) protein. HD is manifested by personality changes, movement disorders and/or dementia. Prior evidence exists for sterol dysregulation in HD models, observed both in neurons and astrocytes. In the brain the cholesterol is produced in situ, as the blood brain barrier prevents the cholesterol metabolized in the periphery to be used by the brain cells. The consensus stablished supports the idea that neurons synthesize the cholesterol needed in the soma, but rely on the cholesterol delivered by astrocytes in the form of apolipoproteins at synapses. A key player studied to be responsible to communicate the cholesterol status of neurons to astrocytes is the 24S-hydroxycholesterol (24S-OHC) also knows as the cerebrosterol (the brain oxysterol). Aim: Prior results from the lab showed cholesterol accumulation in primary striatal neurons infected with lentiviral expression vectors encoding a mutant Htt fragment (Htt17182Q) compared to cells expressing a wild-type Htt fragment (Htt17118Q). Following on these results and the previous published work from different laboratories, the aim of this thesis was to investigate the sterol status of HD striatal neurons in order to better understand this process and the consequences for the neurons and astrocytes’ health in the context of HD. Methods: Filipin staining, cholesterol oxidase assay, isotope-dilution gas chromatographymass spectrometry, NeuN positive staining cell count – neuronal cell death assay, Mitosox assay, Fluo-4 AM imaging, extracellular multi-electrode array recordings, immunocytochemistry and real-time quantitative PCR. Results: I observed sterol accumulation in primary striatal neurons expressing mutant Htt, which one release into the medium significantly increased levels of 24S-OHC. The cerebrosterol is toxic to striatal neurons and the mechanism elucidated was by inducing increased superoxide levels in the mitochondria. Besides the toxicity effect, 24S-OHC was observed to sensitize N-Methyl-D-aspartate (NMDA) receptor function, measured by increased calcium influx in the presence of NMDA and increased amplitude and frequency of firing in the presence of glutamate, modulating NMDA receptor activity. In addition, the studies collected support that 24S-OHC induces sterol dysregulation in astrocytes by downregulating SREBP2 target genes and the purposed mechanism is by blocking the transcription factor’s migration into the nucleus, in this way promotes inhibition of cholesterol and its precursors synthesis. In parallel 24S-OHC up-regulates LXR target genes aiming to increase cholesterol efflux. Conclusion: The results part of this thesis conveyed with others’ published work and brought novelty into the field. Like others I observed sterol accumulation at the plasma membrane and in lysosomes and stablished a link with the sterol dyshomeostasis observed in my model with HD hallmarks – mitochondrial stress and excitotoxicity, caused by the increased of 24SOHC efflux. In addition, I was also able to link the sterol dysregulation in neurons with astrocyte’s cholesterol dyshomeostasis, as evidenced by a different group. This thesis provides new insights into the potential aetiopathogenic mechanisms of HD and reconcile some apparently disparate previous findings regarding sterol disposition in this disorder.
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Zhu, Hong. "Numerical studies of diffusion in lipid-sterol bilayer membranes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ64487.pdf.
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Zhu, Hong 1975. "Numerical studies of diffusion in lipid-sterol bilayer membranes." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=30776.
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We examine tracer diffusion in lipid bilayer membranes containing either cholesterol or lanosterol, using a random lattice Ising model. Specifically the model is a two-state off lattice tethered network of hard disks which is dynamically triangulated and the interactions between the hard disks are only effective along the tethers linking the disks. The model was already applied to lipid-sterol systems and was successful in reproducing the phase diagrams and related physical properties. In this thesis we apply this model in conjunction with Monte Carlo simulation methods as follows. We calculate the diffusion constant for lipid-cholesterol and lipid-lanosterol bilayer membranes both as function of temperature and sterol concentration in all accessible regions of the relevant phase diagrams. Comparison with experiment and comments on sterol related evolution are included.
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Stengl, Helena [Verfasser]. "Mechanismen tDCS-induzierter Lernverbesserung – der Einfluss Serotonins / Helena Stengl." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2019. http://d-nb.info/1202044557/34.
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Serrano, Marco. "Stencil socialista: sistema iconográfico en stencil para la juventud socialista de Chile." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/101041.
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El presente proyecto pretende lograr a través de alternativas gráficas contemporáneas que la retórica del Partido Socialista de Chile se abra al imaginario juvenil contemporáneo. Otorgando un nuevo valor a la comunicación visual de la Juventud Socialista, a través de los códigos y el lenguaje contemporáneo del Stencil, de manera de posicionarlo como un partido moderno y vigente en el tiempo. Se definira la técnica Stencil, analizar sus características morfológicas e implicancias en la sociedad actual. Utilizare la técnica Stencil para pintar los diseños en los muros de las cuatro comunas socialistas de Santiago. Se generaran mensajes comunicacionales a partir del uso de la retórica de la imagen. Luego de atraer la atención de adolescentes y jóvenes santiaguinos con éstas atractivas propuestas aplicadas como módulo de repetición optimizare el tiempo de ejecución de los mensajes, comparativamente, respecto de otras técnicas de pintado utilizadas en la actualidad en los muros.
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Dailida, Robertas. "Stabdžių bandymo stendo modernizavimas." Bachelor's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140716_105956-24937.
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Dviračių stabdžių bandymo stendas yra skirtas, nustatyti įtvirtinto dviračio stabdžių
efektyvumui. Jų veikimą esant skirtingomis oro sąlygomis (lietus, purvas ir t.t.). Dviračio
slydimo atstumui ir stabdant stabdančiūjų dalių susidevėjimui. Šis stendą modernizuojame
pritaikant greičio matuoklį, kuris nustato besisukančio (bandomojo dviračio) rato greitį.
Įrenginys gaminamas iš paprastų medžiagų, kurias būtų galima nusipirkti metalu
prekiaujančiose įmonėse. Standartinius gaminius (tokius kaip guoiai, holo davikliai ir t.t.)
taipogi galima įsigyti specializuotuose parduotuvėse. Būtent gaminamoms detalėms
naudojamos medžiagos – plienas C45, konstrukcinis plienas S235, kaprolonas. Įrenginio
gamybai nereikia didelio kiekio medžiagų, taigi medžiagos bus naudojamos iš likučių
esančių gamyklos sandelyje.
Reikės suprojektuoti ir pagaminti, stabdžių bandymo stendui, besisukančio rato
greičio matuokį, kuris bandymu metu leis nustatyti bandomojo dviračio besisukančio rato
greitį. Ratukas yra pristumiamas, prie bandomojo rato, 400N jėga. Pagal gautus
skaičiavimus skersinė jėga ašelę veikia – 128 N jėga, o lenkimo momentas tik 4 N/m, tai
nesukels konstrukcijoje jokių žymių deformacijų. Dviračių stabdžių stendo modernizacija
reikalinga – dėl to, kad bandymų stendas naudojamas, bandymų centre, kuriame tikrinama
naujų gaminių kokybė. Šie bandymai turi atitikti standartus, o standartai reikalauja... [toliau žr. visą tekstą]<br>The theme of this bachelor project of Mechanical Engineering is contemporary, i.e. about the latest technology in modern industry, where everything is fully computerized and systematized. In this bachelor project we analyze the modernization of bicycle brake stand. The main purpose of this technical modernization is to create and adapt a device which measures the speed of spinning wheel of trial bike. As the brake stand is used for investigational purposes, during the tests, we have to follow standards (LST 14766) which we use to create design of the device. The device is designed to be an open, easily accessible place. Freely access was necessary in order to connect the device to a computer, which treated the information and performed calculations. In this project, we will examine several different options for analysis. After selecting the optimal one, we will try to perform technical calculations. Economical analysis showed that manufacturing, i.e. from designing to final product, will take long approximately about seven days. Total manufacturing costs to produce two speed meter devices would be 3386, 92 Lt. Cost of single device – 1693, 46 Lt. The product price can change depending on product standards and designing time.
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Tuckey, Donna M. "The Influence of Age and Sterol-Inhibiting Fungicides on the Sterol and Steryl Ester Composition of SIF Sensitive and Tolerant Non-Target Chlorella Species." Thesis, Virginia Tech, 2001. http://hdl.handle.net/10919/42764.
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1-substituted 1,2,4-triazoles form a class of agricultural chemicals known as sterol-inhibiting fungicides (SIFs). These fungicides function through the inhibition of sterol synthesis, which ultimately affects cell membrane fluidity and permeability. Of the two main types of sterols in plants, free sterols (FSs) are thought to be incorporated into membranes while conjugated sterols such as steryl esters (SEs), hypothetically, regulate homeostasis by inserting or removing FSs from cell membranes under changing environmental conditions. Non-target algae species possess sterol synthesis pathways that are affected by a range of SIFs. One of the main objectives of the current study was to determine the reason for the observed sensitivity of C. fusca and the tolerance of C. kessleri to SIFs relative to total lipid, FS, SE and FFA composition. These parameters were measured using gas chromatography and mass spectroscopy techniques. Both quantitative and qualitative differences in sterol number and type were noted relative to the FS and SE composition of the two species of algae over time. Notably, SEs were detected in both species of algae, although presence and amount varied with the organism. While SEs were more abundant in C. kessleri, higher amounts of FSs were found in C. fusca. The FS/SE ratios were 64/36 and 88/12 percent of the total sterol in C. kessleri and C. fusca, respectively. Treatment of C. fusca with 2, 4, and 6 ppm and C. kessleri with 6,12, and 24 ppm propiconazole caused an accumulation of methylated precursor sterols, resulting in slightly more FSs in both algae. Only 3 of the FSs produced following treatment were different from the control in C. fusca while 9 new sterols were found in C. kessleri. Treatment also altered the SE fraction in both species, with fewer SEs produced compared to the control, but more novel sterols in C. kessleri, suggesting a possible inverse relationship between FSs and SEs in both organisms. Several studies have implicated lipid/sterol concentrations with the potential for cellular bioaccumulation of lipophilic xenobiotics as they relate to membrane permeability. Cell age and environmental parameters can also affect lipid composition of algae. Although cell age did not affect the qualitative sterol composition of C. fusca and C. kessleri, quantitative differences were observed. Plants exposed to chemical and other environmental stresses accumulate free fatty acids (FFAs), which may be linked to biophysical membrane changes. SIF sensitive C. fusca, had inherently higher levels of FFAs than C. kessleri. Qualitatively, C. fusca exhibited higher percentages of 18:1 and lower ratios of 18:2/18:3 FFAs than C. kessleri. In response to increasing SIF treatment, the ratio of 18:2/18:3 FFAs increased in C. kessleri and declined in C. fusca. The amount of total lipid produced in the cells of C. fusca was higher than in C. kessleri during all growth stages. Variations were observed in lipid measured as a percent dry weight compared to lipid/cell as the cultures age. Inherent differences in FS, SE, and lipid composition of C. fusca and C. kessleri as well as age related changes could account for the differences in the susceptibility of the two algae to propiconazole.<br>Master of Science
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Arnqvist, Lisa. "Plant sterol metabolism with emphasis on glycoalkaloid biosynthesis in potato /." Uppsala : Dept. of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/2007128.pdf.
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Gros, Ludovic. "Sterol 24-methyltransferase as a drug target in parasitic protozoa." Thesis, Cardiff University, 2005. http://orca.cf.ac.uk/55386/.
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In order to investigate further the mode of action of these compounds, synthesis of proteomic probes was attempted. Synthetic design and attempts are presented chapter VII. To establish whether there was 24-SMT present in the blood stream form of T. b. brucei, a Northern blot was carried out. This confirmed transcription of the enzyme which was then cloned, over expressed and purified (Chapter VIII). Enzyme assays were carried out against the recombinant enzyme.
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Kaur, Achint. "The role of sterol 12α- hydroxylase (Cyp8b1) in glucose homeostasis". Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/46516.
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Iddon, Christopher R. "Investigation of the putative sterol pool that regulates SREBP cleavage." Thesis, University of Sheffield, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392930.
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Parker, Stephen R. "Sterol biosynthesis in Aspergillus and its inhibition by azole antimycotics." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307142.
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Wright, Edward A. "Studies on Sterol Metabolism in the Opportunistic Pathogen Pneumocystis carinii." University of Cincinnati / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1367934810.
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Modi, Anuja R. "MUTAGENESIS AND SPECTROSCOPIC STUDIES OF MYCOBACTERIUM TUBERCULOSIS STEROL 14ALPHA DEMETHYLASE." VCU Scholars Compass, 2009. http://scholarscompass.vcu.edu/etd/1912.
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P450s are heme containing enzymes which affect oxidation of substrates via catalytic intermediates having transient lifetimes. These oxidative catalytic intermediates are formed by a sequential interplay of electrons and protons at the active site of the enzyme bearing molecular dioxygen. The proton transfer to the active site from bulk solvent is coordinated by an “acid-alcohol” pair of active site residues which are conserved in all P450s. Sterol 14α-demethylases (CYP51) are P450 enzymes which catalyze oxidative deformylation of lanosterol in the cholesterol/ergosterol biosynthetic pathway. Both cholesterol and ergosterol are important regulators of membrane fluidity. CYP51 differs from other P450s in that the acid in the acid-alcohol pair in the active site is replaced by a His residue. This enzyme is present in tuberculosis (TB) causing pathogen Mycobacterium tuberculosis (Mtb). This finding was significant for primarily two reasons. The first one being the baffling presence of CYP51 in Mtb, as Mtb is not known to have any endogenous sterol biosynthetic pathways. The second being that CYP51 is a validated drug target in treating fungal infections. Thus given the global resurgence of multidrug resistant strains of Mtb and the deadly coexsistence of Mtb in immunocompromised HIV patients, CYP51 may be an ideal drug target for new generation of antimycobacterial drugs. The Mtb CYP51 enzyme was chosen to study the proton transfer pathways in the active site based on the outcome of explicit solvent molecular dynamics and hybrid quantum mechanics/molecular mechanics calculations performed in our laboratory. Based on these calculations of CYP51 catalysis, Glu173 was implicated to be the proton source. Proton transfer to the active site occurred by a coordinated shuttling via four water molecules, His259 and Thr260. To experimentally verify the roles of Glu173, His259 and Thr260 they were mutated to alanine and biophysically characterized. Ferredoxin, an accessory protein required to shuttle electrons from NADPH to the CYP51 active site for catalysis, was also cloned using ligation independent cloning. We were successfully able to reconstitute the electron transport chain for CYP51. The mutants were found to differentially bind type I and type II enzymes. Based on biophysical characterization, Thr260 can be implicated to have a role in modulating the spin state of the enzyme. The Mtb CYP51 enzyme was chosen to study the proton transfer pathways in the active site based on the outcome of explicit solvent molecular dynamics and hybrid quantum mechanics/molecular mechanics calculations performed in our laboratory. Based on these calculations of CYP51 catalysis, Glu173 was implicated to be the proton source. Proton transfer to the active site occurred by a coordinated shuttling via four water molecules, His259 and Thr260. To experimentally verify the roles of Glu173, His259 and Thr260 they were mutated to alanine and biophysically characterized. Ferredoxin, an accessory protein required to shuttle electrons from NADPH to the CYP51 active site for catalysis, was also cloned using ligation independent cloning. We were successfully able to reconstitute the electron transport chain for CYP51. The mutants were found to differentially bind type I and type II enzymes. Based on biophysical characterization, Thr260 can be implicated to have a role in modulating the spin state of the enzyme.
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Montpetit, Alessandro. "Measurement and Separation of Sterol Glycosides in Biodiesel and FAME." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32978.
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The major issue that hinder the widespread use of biodiesel is its poor cold weather stability and operability. This is attributed minor components identified as monoglycerides (MG), diglycerides (DG) and sterol glycosides (SG). There is currently no standard method to determine SG levels in biodiesel.
A method to isolate and measure SG concentration in biodiesel and FAME was first developed. This was accomplished by decompatibilizing SG from the biodiesel matrix using n-dodecane and purifying the solids using a Folch liquid-liquid extraction. The extracted SG was analyzed by GC-FID; the tricaprin internal standard was detected at 21.5 min and SG from 26-26 min. Recovery using this method was 100% ± 2.5% when 3 commercial canola biodiesel samples were spiked with 38 ppm SG and extracted.
This method was used to measure SG concentration of filtered FAME produced using 0.3wt%, 0.5wt% and 0.7wt% at a MeOH:Oil (mol/mol) ratio of 4:1, 5:1, 6:1, 7:1 and 9:1. The biodiesel produced was characterized according to ASTM D6584; MG, DG and TG decreased with increasing catalyst concentration and MeOH:Oil ratios. The SG solubility in reactive FAME was found to be lowest at high glycerol catalyst concentration. High levels of TG were found to solubilize SG in the reactive FAME. Finally, the solubility of SG in reactive FAME increased when high ratios of methanol were used.
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Voght, Stephen P. "Establishment of a Drosophila model of intestinal sterol absorption and trafficking /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/10303.
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Miciak, A. "The effects of some cyclohexanol derivatives on rat hepatic sterol synthesis." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355294.
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Haughan, Penelope. "Action of paclobutrazol and the sterol requirement for plant cell growth." Thesis, University of Liverpool, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237512.
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Blanc, Mathieu. "Sterol biosynthesis pathway is part of the interferon host defence response." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/5556.
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Recently, cholesterol metabolism has been shown to modulate the infection of several viruses and there is growing evidence that inflammatory response to infection also modulates lipid metabolism. However little is known about the role of inflammatory processes in modulating lipid metabolism and their consequences for the viral infection. This study investigates host-lipid viral interaction pathways using mouse cytomegalovirus, a large double-stranded DNA genome, which represents one of the few models for a natural infection of its natural host. In this study, transcriptomic and lipidomic profiling of macrophages shows that there is a specific coordinated regulation of the sterol pathways upon viral infection or treatment with IFNγ or β (but not TNFα, IL1β or IL6) resulting in the decrease of free cellular cholesterol. Furthermore, we show that pharmacological and RNAi inhibition of the sterol pathway augments protection against infection in vitro and in vivo and we identified that the prenylation branch of the sterol metabolic network was involved in the protective response. Finally, we show that genetic knock out of IFNβ results in a partial reduction while genetic knock out of Ifnar1 completely abolishes the reduction of the sterol biosynthetic activity upon infection. Overall these results support a role for part of the sterol metabolic network in protective immunity and show that type 1 IFN signalling is both necessary and sufficient for reducing the sterol metabolic network upon infection; thereby linking the sterol pathway with IFN defence responses.
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BERTOLIO, REBECCA. "Sterol Regulatory Element Binding Protein couples mechanical cues to lipid metabolism." Doctoral thesis, Università degli Studi di Trieste, 2020. http://hdl.handle.net/11368/2963753.
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Sterol regulatory element binding proteins (SREBPs) are a family of transcription factors that regulate lipid biosynthesis and adipogenesis by controlling the expression of several enzymes required for endogenous cholesterol, fatty acid, triacylglycerol, and phospholipid synthesis1. In vertebrates, SREBP activation is mainly controlled by a complex and well-characterized feedback mechanism mediated by cholesterol, a crucial bio-product of the SREBP-activated mevalonate pathway.
In this work, we identified the acto-myosin contractility and mechanical forces imposed by the extracellular matrix (ECM) as SREBP1 regulators. The control of SREBP1 by mechanical cues depends on geranylgeranyl pyrophosphate, another key bio-product of the mevalonate pathway, and impacts on stem cell fate in mouse and on fat storage in Drosophila melanogaster.
Mechanistically, we show that activation of AMP-activated protein kinase (AMPK) by ECM stiffening and geranylgeranylated RhoA-dependent acto-myosin contraction inhibits SREBP1 maturation and functional activation. Our results unveil an unpredicted and evolutionary conserved role of SREBP1 in rewiring cell metabolism in response to mechanical cues.
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Hesselbrandt, Max. "Luktreducering av stekos med absorptionsteknik." Thesis, KTH, Energiteknik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-170890.
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In exhaust and supply air ventilation with heat recovery, heat and moisture is transferred by a heat exchanger from the warm exhaust air flow to the cold supply air flow. Rotary regenerators are widely used due to their high efficiency, but have the drewback of transferring odourants from the kitchen hood exhaust air to the supply air. This causes odour problems that must be prevented. In this study, odour control by absorption has been studied theoretically in order to investigate whether it is possible to efficiently remove odorants from the kitchen hood exhaust air and minimizing heat losses simultaneously during the absorption process. By studying the olfactory system, some physicochemical properties of odorant molecules were identified. Furthermore, the mechanisms of mass and heat transfer were considered in order to identify the factors that affect the rate of mass and heat fluxes between the liquid and gas phases. Also, the main factors involving the design of packed columns were investigated. The study showed that there are several similarities between mass and heat transfer. Thus, some of the factors that were found to increase the rate of mass transfer also tend to cause an increase of heat transfer. Therefore, it is concluded that other options than absorption should be considered.<br>I FTX-system återvinns värme och fukt i den varma inomhusluften och överförs i en värmeväxlare till den kalla uteluften. För detta syfte används vanligen den roterande regenerativa värmeväxlaren på grund av dess höga temperaturverkningsgrad, men nackdelen med denna typ av värmeväxlare är att luktämnen från köksventilationen kan överföras till den inkommande tilluften. Detta orsakar luktproblem och måste förhindras. Syftet med denna studie är att undersöka huruvida absorption kan användas för att effektivt rena spiskåpans frånluft från luktämnen och andra föroreningar samtidigt som eventuella värmeförluster minimeras. Genom att studera luktsinnets funktion har de fysiokemiska egenskaper som är karaktäristiska för luktämnen kartlagts. Vidare har mekanismerna bakom mass- och värmeöverföring tagits i betraktande i syfte att identifiera de faktorer som påverkar graden av mass- och värmeflöden mellan gas- och vätskefas. Studien innefattar även den tekniska utformningen av en packad kolonn. Studien visar att det finns en tydlig analogi mellan mass- och värmeöverföring. Detta innebär att några av de faktorer som bidrar till en högre grad av massöverföring också tenderar att öka graden av värmeöverföring. Således kan en slutsats dras om att absorption som metod inte är optimal i detta avseende, varför andra lösningar bör övervägas.
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CHAPIER, JEAN-MARIE. "Lp(a) et stenose coronarienne." Strasbourg 1, 1991. http://www.theses.fr/1991STR15065.
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West, Kristi Lee. "Ecology and biology of the rough-toothed dolphin (Steno bredanensis)." Thesis, University of Hawaii at Manoa, 2002. http://hdl.handle.net/10125/990.
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Greater knowledge of the rough-toothed dolphin, Steno bredanensis, is needed to effectively contribute to conservation and management efforts for this species. The primary purpose of this research was to describe ecological and biological parameters for S. bredanensis that will be useful in future assessments of population stress. Several approaches were used to study S. bredanensis, including investigations of free-ranging populations, dead specimens, and captive individuals. Free-ranging rough-toothed dolphins distributed near small oceanic island environments were found to be more commonly sighted in-shore than off-shore. In the Windward islands of French Polynesia, this species preferred water depths of 1000 to 2000m and a distance of 1.8 to 5.5 km from the barrier reef. Group sizes ofrough-toothed dolphins sighted in French Polynesia range between 1 and 35 individuals with a mean size of 12.1. Endocrinology data for S. bredanensis was established in captive healthy and stranded individuals. Ranges and means were provided for progesterone, testosterone, cortisol and thyroid hormones. Changes in thyroid hormone concentrations were reflective of health status and testosterone appeared to be suppressed in ill individuals. Reproduction in S. bredanensis was investigated by determining the size and age range that this species attains sexual and physical maturity. Female rough-toothed dolphins attain sexual maturity by 9 to 10 years of age and males between 5 and 10 years at a similar length of approximately 216 cm. Physical maturity is generally reached at an older age and larger size for both males and
females. Ecologically healthy and unheahhy populations of S. bredanensis were described in this investigation and these fmding will be useful in assessing future threats to this species.
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West, Kristi L. "Écologie et biologie du dauphin à bec étroit (steno bredanensis)." Polynésie française, 2002. http://www.theses.fr/2002POLF0001.
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L'écologie et la biologie du dauphin steno bredanensis sont étudiées pour mieux évaluer le niveau de stress des populations et aider à la protection de l'espèce. En milieu insulaire, les populations ont été plus fréquemment observées près des côtes qu'au large, dans des zones où la profondeur varie de 1000 à 2000 mètres et à une distance de 1,8 à 1,5 km du récif. Le nombre moyen d'individus par groupe est de 12,1. Les taux de progestérone, de testostérone, de cortisol et d'hormones thyroi͏̈diennes ont été établis pour des dauphins en bonne santé en captivité et pour des animaux échoués ; la testostérone est généralement basse chez les animaux malades. L'âge à première maturité sexuelle est de 9 à 10 ans chez les femelles et de 5 à 10 ans pour les mâles, pour une taille moyenne de 216 cm. La maturité est plus tardive pour les deux sexes. La comparaison entre populations en bonne et en mauvaise santé sera utile pour évaluer les menaces qui pourraient peser sur cette espèce<br>In French Polynesia, steno bredanensis was found to prefer water depths of 1000 to 2000 m at a distance of 1,8 to 5,5 km from the barrier reef. Group sizes of rough-toothed dolphins sighted in French Polynesia range between 1 and 35 individuals with a mean size 12,1. Endocrinology data for steno bredanensis was established in captive healthy and stranded individuals. Ranges and means were provided for progesterone, testosterone, cortisol and thyroid hormones. Changes in thyroid hormones concentrations were reflective of health status and testosterone appeared to be surppressed in ill individuals. Reproduction in steno bredanensis was investigatited by determining the size and age range that this species attains sexual and physical maturity. Femal rough-toothed dolphins attain sexual maturity by 9 to 10 years of age and males between 5 and 10 years at the similar length of approximately 216 cm. Physical maturity is attained at a greater size and age in both sexes
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Frescatada-Rosa, Márcia. "Dissecting sterol function during clathrin-dependent endocytosis and cytokinesis in Arabidopsis thaliana." Doctoral thesis, Umeå universitet, Umeå Plant Science Centre (UPSC), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-80030.
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Sterols are lipid components of eukaryotic membranes. Alterations of membrane sterol composition perturb the execution of cell division, which in diverse eukaryotes can have severe consequences for development of the organism. Partitioning of the cytoplasm during cell division occurs at the final stage of cell division named cytokinesis. In somatic plant cells, cytokinesis is initiated by fusion of membrane vesicles in the plane of cell division resulting in a transient compartment termed the cell plate. Cell plate maturation relies on temporal and spatial orchestration of membrane fusion and endocytosis. Impaired vesicle fusion or defects in endocytosis result in cytokinetic defects. In Arabidopsis thaliana, the KNOLLE and DYNAMIN-RELATED PROTEIN 1A (DRP1A) contribute to cytokinesis. KNOLLE mediates fusion of vesicles at the plane of cell division while DRP1A appears to be involved in cell plate maturation through its role in clathrin-mediated endocytosis. This thesis shows that KNOLLE is specifically restricted to the cell division plane through sterol-dependent endocytosis that involves a clathrin- and DRP1A-mediated mechanism. Sterols affect internalization of KNOLLE through their role in lateral membrane organization by keeping diffusion of KNOLLE to lateral membranes in check via its endocytic removal. It is shown that the cell plate represents a high-lipid-order membrane domain that depends on the correct composition and the right concentration of sterols. Accumulation of DRP1A at the cell plate requires correct sterol concentration and composition similar to high-lipid order. Conversely, high-lipid-order at the cell plate relies on DRP1A activity suggesting a feedback between DRP1A function and lipid order establishment. Finally, it is shown that sterols are also present at the tonoplast of dividing and elongated root cells. Taken together, the results reveal that formation of the cell plate in Arabidopsis thaliana depends on an intricate interplay between cytokinetic vesicle fusion, sterol-dependent lateral membrane and high-lipid-order domain organization as well as endocytic machinery function.
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Miller, Luke Anthony Damien. "Development of sterol aster analysis for establishing the authenticity of edible oils." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295315.
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Lundén, Amanda. "Can Sterol Carrie Protein-2 function as a solubility tag in E.coli?" Thesis, Linköpings universitet, Biologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-129803.
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Expressing foreign proteins in E.coli is a major challenge because they often tend to develop into unsolvable and inactive proteins. They aggregate into so called inclusion bodies which prevent expression of the protein. This problem might be avoided by fusing the gene of the foreign protein with a soluble protein called solubility tags, which function is to enhance the solubility of the foreign protein. This report investigates whether Sterol Carrier Protein-2 (SCP-2) could function as a solubility tag. The experiment was carried out by fusing SCP-2 to two recombinant proteins, Green fluorescent protein (GFP) and a form of chloroamphenicol acetyl transferase (CATΔ9). The gene fusion was then inserted into a pET-15 vector and transformed into the E.coli strain BL21(DE3) to be expressed. The results obtained from Western blot and PageBlue staining indicates that SCP-2 does not enhance the solubility of GFP or CATΔ9 since neither of them was expressed. Furthermore, previous studies have shown that GFP can in fact be expressed usingmaltose binding protein (MBP) as a solubility tag. Unfortunately, no success has been made regarding CATΔ9. In conclusion, regarding the results from this report, SCP-2 does not function as a solubility tag. However, further studies should be carried out on SCP-2 with more experiments before rejecting the possibility to use SCP-2 as a solubility tag.
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Nishino, Tomohiro. "MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice." Kyoto University, 2016. http://hdl.handle.net/2433/215426.
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Shillito, Alex Benjamin. "How the Heart Became Muscle: From René Descartes to Nicholas Steno." Scholar Commons, 2019. https://scholarcommons.usf.edu/etd/7939.
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This dissertation addresses the heartbeat and the systems of natural philosophy that were used to explain it in the 17th century. Thus, I work in two domains of explanation. The first domain is physiology, in which William Harvey correctly ordered the heart’s systolic and diastolic motions, while René Descartes incorrectly reversed them. By looking at Harvey and Descartes’ more complete physiological models I reconsider the controversy that spun out of their divergent accounts. The second domain is the junction of physics and metaphysics, representing the frameworks of natural philosophy behind physiology. I argue that Harvey’s physiology was correct while his supporting principles were “wrong,” and Descartes’ physiology was incorrect while his supporting principles were “right.” Thus, my thesis is that Harvey was “right” but perhaps for the wrong reasons, while Descartes was “wrong” but perhaps for the right reasons. Of course, this judgement is made from a contemporary perspective. By using a contextualist approach to history, I aim to show how the controversy between Harvey and Descartes resolved in Nicolas Steno, when he discovered that the heart is a muscle.
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Choubey, Animesh. "Grain boundary etched 316L stainless steel (SS) surfaces for cardiovascular stents a dissertation /." San Antonio : UTHSC, 2008. http://proquest.umi.com.libproxy.uthscsa.edu/pqdweb?did=1594485681&sid=3&Fmt=2&clientId=70986&RQT=309&VName=PQD.
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Jučas, Tomas. "Elektros tinklo valdymo laboratorinio stendo prototipas." Bachelor's thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20120702_113342-54397.
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Parengtas bakalauro baigiamasis darbas yra skirtas suprojektuoti ir pagaminti automatinio rezervo įjungimo skydo prototipą, kurį būtų galima naudoti laboratoriniams darbams universitete.Šio darbo paskirtis yra papildyti elektros aparatų discipliną dar vienu, dažnai sutinkamu ir naudojamu visame pasaulyje stendu, paaiškinant jo veikimo principą, galimybes. Darbe aiškiai yra išdėstomos visos stendo dalys, stendui keliami reikalavimai, parodomos principinė ir valdymo schemos, paaiškinamas detalus veikimas, stendo valdiklio programos algoritmas. Taip pat supažindinama su Lucas-neulle mokomuoju dviejų atskirų tiekimo linijų stendu, parodant visas jo sudedamąsias dalis ir jas trumpai paaiškinant.<br>ATS helps to avoid serious problems in healthcare facilities for example by the same time when surgery is being held to save human lives and power supply interference occurs, it safely automatically switches power supply mains with no effect to the user. In industry it helps to avoid any power supply problems which every single of them could lead to destruction of expensive machinery or even harm people. Considering problems occurring in power supply lines, this was a great inspiration to produce automatic transfer switch prototype as my Bachelors degree thesis. Since Electrical Engineering Department had no real ATS system prototype before, it will surely supply existing basis and will help students to gain useful experience. Furthermore, all the details about ATS are expounded and will lead to full understanding of the system.
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Kaubrys, Evaldas. "Dažnio matavimo stendo sudarymas ir tyrimas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140616_164417-07979.
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Dažnio matavimo stendo sudarymas ir tyrimas. Magistro baigiamasis darbas elektronikos inžinerijos laipsniui. Vilniaus Gedimino technikos universitetas. Vilnius, 2014, 71 p., 31 iliustr., 3 lent., 36 bibl., 6 priedai. Sukurtas ir ištirtas dažnio matavimo stendas, atskleidžiantis skaitmeninių dažnio ir periodo matavimo būdų ypatumus. Stendas pakeis šiuo metu laboratoriniuose darbuose naudojamus pasenusius prietaisus. Stendą sudaro 3 užduodantys generatoriai, dažnio sintezatorius, dažniamatis ir sąsaja su asmeniniu kompiuteriu, bendrame korpuse. Matavimo rezultatams įvertinti sukurta specializuota programinė įranga. Atlikus stendo tyrimus įsitikinta, kad sukurtas dažniamačio stendas atitinka visus užduoties reikalavimus.<br>Creation and investigation of frequency counter. Master's thesis in electronics engineering degree. Vilnius Gediminas Technical University. Vilnius , 2014, 71 p., 31 pictures, 3 tables, 36 references, 6 extras. Frequency counter stand have been created and investigated. Stand will allow students to familiarize themselves with particularities of digital measurement methods of frequency and duration of the period. The new stand will replace old measurement devices that are used during laboratory works recently. The stand consists of frequency synthesizer, frequency counter, 3 different reference generators and interface to the computer packed in single case. Specialized software have been created to assess and treat measurements results. Testing results of the bench proved that created frequency counter stand meets all the requirements of the task.
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Mansukhani, Kirti Ramesh 1981. "A haptic stencil for manufacturing applications." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/27037.
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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.<br>Includes bibliographical references (p. 86-90).<br>The haptic stencil consists of a 5 DOF haptic device and an anti-collision algorithm that acts as a geometric stencil and can be used for a variety of applications ranging from training to rapid prototyping and manufacturing. Online manipulation of a three-axis desktop milling machine was established using this setup. This work describes the algorithm design used to achieve the required performance and stencil-like behavior with specific reference to machining applications. Some of the primary aspects of this design include the collision detection, collision remediation and control methodologies employed. The parameters on which these methodologies depended and how they were developed are the focus of this work. Collision detection is the core of any haptic interaction as it determines whether or not contact has been established between the virtual objects and therefore is essential in deciding the appropriate haptic feedback. In the case of the haptic stencil, the collision detection algorithm would have to identify whether or not contact occurs between the haptic probe-controlled tool object and the stationary part object. Collision remediation provides the stencil-like behavior by enforcing geometric constraints on the regions/surfaces by preventing penetration by the tool object. The results from the collision detection and collision remediation modules are used to control the desktop milling machine which cuts out a copy of the part object used in the haptic simulation from a given stock according to the motions specified on the haptic probe by the operator. Speed control is necessary in order to ensure that motions from the human operator are not lost due to the different communication speeds between the various modules of this setup.<br>(cont.) Speed control also helps in providing as 'real-time' a machining experience as possible for a given part and stock combination.<br>by Kirti Ramesh Mansukhani.<br>S.M.
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Traiven, Charlie. "Stonehenge-mer än bara stora stenar." Thesis, Uppsala universitet, Institutionen för arkeologi och antik historia, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-307412.
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Stonehenge is a place of mystery and wonder, where it stands as a last witness to long forgotten religious practices and rituals, and its sophisticated stone structure still makes a huge impression after over 4000 years. And it raises questions as to how it was built, and why? Stonehenge is today one of the world`s most famous megalithic monument in the world, and in its right. But Stonehenge is more than just big stones;it has a much longer and richer history than that. Stonehenge also has many surrounding monuments, from the same time period, and thought of as today, to coexist and fill different, specific functions, as a ritual landscape. The more archaeologists learn about Stonehenge, the more complex the picture gets. Today, the understanding of Stonehenge lies just as much in the surrounding landscape, as in the monument itself.
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Toussaint, Marcel. "Histologie myocardique dans la stenose pulmonaire." Paris 6, 1987. http://www.theses.fr/1987PA066029.
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Rawat, Prashant Singh. "Optimization of Stencil Computations on GPUs." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1523037713249436.
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