Relevant bibliographies by topics / Sterile medium without autoclaving

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Academic literature on the topic 'Sterile medium without autoclaving'

Author: Grafiati
Published: 3 June 2025
Last updated: 20 July 2025

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Journal articles on the topic "Sterile medium without autoclaving"

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Kovtun, Yu S., A. A. Kurilova, L. S. Katunina, and E. I. Vasilenko. "Assessment of the Use of Gentian Violet and Malachite Green as Selective Agents in the Isolation of Brucella." Problems of Particularly Dangerous Infections, no. 3 (October 23, 2021): 60–65. http://dx.doi.org/10.21055/0370-1069-2021-3-60-65.

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The aim of the work is to evaluate ability of gentian violet and malachite green to inhibit 2 Brucella test strains and 8 strains of unwanted organisms and the influence of autoclaving on their activity. The study also aims to evaluate variability of inhibition in different batches of colorants and necessity in adjustment of their concentration.Materials and methods. The study included 3 imported lots of gentian violet and malachite green. Inhibitors were put into nutrient medium containing pancreatic hydrolysates of gelatin and fsh meal, yeast extract, sodium chloride, glucose, sodium pyrosulfa
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Tsafouros, Athanasios, and Petros A. Roussos. "In Vitro Propagation of Commercially Used Krymsk 5® (Prunus fruticosa × Prunus lannesiana) Cherry Rootstock: Impact of Sugar Types and pH Levels." Agriculture 14, no. 1 (2024): 120. http://dx.doi.org/10.3390/agriculture14010120.

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In the present study, the effects of different types of sugars and cultivation medium pH levels on the micropropagation of Krymsk 5® cherry rootstock were investigated. During the proliferation stage, the effects of four sugars (sucrose, fructose, glucose, and sorbitol) both separately and in two combinations were studied, along with the effects of pre-adjusted pH (4.5, 5.0, 5.2, 5.5, 5.8, 6.0, 6.2, or 6.5) on shoot proliferation parameters, growth medium’s post-autoclaving and post-cultivation pH, and their relations. Similarly, during the rooting stage, the effects of four sugars (sucrose, g
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Terrones-Fernandez, Ines, Lourdes Rodero-De-Lamo, Asunción López, et al. "Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap." Applied Sciences 14, no. 6 (2024): 2340. http://dx.doi.org/10.3390/app14062340.

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There is currently increased interest in the use of alternatives to autoclaved culture media, in order to maintain the properties of the media, while saving energy and time. In this study, we assess a new system for culture media preparation, using a conventional microwave with a water bath and a glass bottle with a rubber cap that allows depressurization. Sterilization, using the proposed system (1000 W, 3 to 20 min), was compared with autoclaving for the preparation of tryptone soy agar (TSA), tryptone soy broth (TSB), Sabouraud 4% dextrose agar (SDA), and violet red bile glucose agar (VRBG)
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McConnell, J. "Two Tropical Plants as Plant Materials for Teaching Seed Germination." HortScience 31, no. 4 (1996): 650a—650. http://dx.doi.org/10.21273/hortsci.31.4.650a.

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A local ground orchid, Spathoglottis plicata Blume, and coconut, Cocos nucifera L., were used in the classroom to teach seed germination. S. plicata, a common orchid on Guam, was utilized to demonstrate the aseptic culture of seeds under non-sterile conditions. The procedures were done in the classroom without a laminar air-flow cabinet. Nonsterile seeds were sown on growing media which were prepared without autoclaving, but by incorporating sodium hypochlorite into the media. Students had a high rate of success in germinating the orchid seeds without contamination by spraying sodium hypochlor
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Ribeiro, Juliana Martins, Joselita Cardoso de Souza, Brenda Lima Ribeiro, et al. "Aseptic manipulation of cultures outside the laminar flow hood." Australian Journal of Crop Science, no. 18(05):2024 (May 18, 2024): 248–52. http://dx.doi.org/10.21475/ajcs.24.18.05.p3009.

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In the present study, laminar flow hoods were used as air filters in inoculation rooms. This study was developed in two stages. First, on a bench inside the laboratory inoculation room, with surfaces that had been cleaned with water and bleach; and second with air filtered by laminar flow hoods inside the room. In the first stage, the growth medium was sterilized by autoclaving and contamination was analyzed in culture flasks without explants. The tested media remained open on a bench for 10 minutes in an environment where laminar flow hoods were previously operated at different time ranges (2
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Wang, Haifeng, Juan Guo, Xing Chen, and Hongxuan He. "The Metabolomics Changes in Luria–Bertani Broth Medium under Different Sterilization Methods and Their Effects on Bacillus Growth." Metabolites 13, no. 8 (2023): 958. http://dx.doi.org/10.3390/metabo13080958.

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Luria–Bertani broth (LB) culture medium is a commonly used bacterial culture medium in the laboratory. The nutrient composition, concentration, and culture conditions of LB medium can influence the growth of microbial strains. The purpose of this article is to demonstrate the impact of LB liquid culture medium on microbial growth under different sterilization conditions. In this study, LB medium with four different treatments was used, as follows: A, LB medium without treatments; B, LB medium with filtration; C, LB medium with autoclaving; and D, LB medium with autoclaving and cultured for 12
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Vallejo-Labrada, Maricela, and Juan Carlos Ojeda-Garces. "Microbiological Study of Cast Posts before Cementation." International Journal of Dentistry 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/1090534.

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This study identifies the most common microorganisms present in type III gold cast posts related to pulpal disease and evaluates the sterilization/disinfection method before cementation in the root canal. Forty-five type III gold cast posts were aseptically collected in sterile sealed plastic bags and taken to the microbiology laboratory to carry out the study: fifteen cast posts had no treatment, fifteen were disinfected (immersion in 70% alcohol during 15 minutes), and fifteen were autoclaved at 121°C for 15 minutes by using saturated steam under 15 psi pressure. By using a two-proportionz-t
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Bleakley, Bruce H., and Xiang Chen. "Survival of insect pathogenic and human clinical isolates ofPhotorhabdus luminescensin previously sterile soil." Canadian Journal of Microbiology 45, no. 3 (1999): 273–78. http://dx.doi.org/10.1139/w98-231.

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Most characterized strains of the bacterium Photorhabdus luminescens are symbionts of entomopathogenic nematodes, whereas other strains have been isolated from human clinical specimens. The ability of P. luminescens strains to survive and grow in soil has received limited attention, with some studies indicating these bacteria have little or no ability to persist in soil. Survival and (or) growth of P. luminescens strains in previously sterilized soil, and examination of different soil amendments on their numbers in soil, have not been previously reported. Entomopathogenic P. luminescens (ATCC
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Perica, Mirna Curkovic, and Jasna Berljak. "In Vitro Growth and Regeneration of Drosera spatulata Labill. on Various Media." HortScience 31, no. 6 (1996): 1033–34. http://dx.doi.org/10.21273/hortsci.31.6.1033.

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Conditions for in vitro multiplication and flowering of Drosera spatulata plants were established. Shoot tips of greenhouse-grown plants were sterilized with 1% or 0.5% sodium hypochlorite. The influence of different media concentrations, hormone supplementation, and pH was investigated. Full MS medium without growth regulators was the best for regeneration and multiplication of plants. Regenerated shoots rooted spontaneously on medium without growth regulators and without transfer to additional medium. In 3 months, 100 to 200 plants were generated per explant. Flowering was induced on media s
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Cardoso, Jean Carlos, and Ana Carolina Petit Inthurn. "Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)." Ornamental Horticulture 24, no. 3 (2018): 218–24. http://dx.doi.org/10.14295/oh.v24i3.1222.

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Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlorine dioxide (ClO2) for in vitro cultivation of gerbera. There was used gerbera in vitro shoots in the stage of rooting for these experiments, using 0.0035%, 0.0070% and 0.0105% of chlorine dioxide in the culture medium. Also, peracetic acid was tested previously for sterilization, but resulted in micr
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Conference papers on the topic "Sterile medium without autoclaving"

1

Ocando, Lisseth, Matilde F. de Romero, Oladis T. de Rincón, et al. "Comparative Study of the Effect of Sulfate-Reducing Bacteria on Cathodic Protection under Simulated Blisters." In CONFERENCE 2023. AMPP, 2023. https://doi.org/10.5006/c2023-19465.

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Abstract This work presents a comparison of the effect generated by a mixed culture of sulfate-reducing bacteria (SRB) isolated from a natural estuary in western Venezuela, on the cathodic protection (CP) of carbon steel under simulated blisters. The laboratory study was carried out simultaneously measuring attack morphology, polarized potential, pH, and sessile growth, inside a simulated blister in a special cell arrangement, using two types of coatings: one 100% solids epoxy, and the other one, a tape type, which consists of the combination of a geo mesh-polypropylene textile and tarred rubb
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Ocando, Lisseth, Oladis Troconis de Rincón, Matilde F. de Romero, and Antonio De Turris. "Comparative Study of the Effect of Sulfate-Reducing Bacteria on Cathodic Protection under Simulated Blisters." In CONFERENCE 2024. AMPP, 2024. https://doi.org/10.5006/c2024-21195.

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Abstract This work presents a comparison of the effect generated by a mixed culture of sulfate-reducing bacteria (SRB) isolated from a natural estuary in western Venezuela, on the cathodic protection (CP) of carbon steel under simulated blisters. The laboratory study was carried out simultaneously measuring attack morphology, polarized potential, pH, and sessile growth, inside a simulated blister in a special cell arrangement, using two types of coatings: one 100% solids epoxy, and the other one, a tape type, which consists of the combination of a geo mesh-polypropylene textile and tarred rubb
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3

Padilla-Viveros, A. A., E. García-Ochoa, R. García-Esquivel, D. Alazard, M.-L. Fardeau, and B. Ollivier. "The Influence of the Culture Media on the Steel Corrosion Induced by Desulfovibrio vulgaris subsp. oxamicus: An Electrochemical Noise Study." In CORROSION 2006. NACE International, 2006. https://doi.org/10.5006/c2006-06522.

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Abstract The corrosion processes induced by Desulfovibrio vulgaris subsp. oxamicus (DSM192T) on carbon steel growing in lactate/sulfate and in lactate/nitrate media were analyzed through the use of electrochemical noise technique. Like some other sulfate-reducing bacteria, this Desulfovibrio species was able to reduce nitrate and produced ammonia in the absence of sulfate. Sterile control of lactate/sulfate culture medium without bacteria did not show any significant change in localization index (LI) evolution. However, the sterile control of lactate/nitrate culture medium showed the occurrenc
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Pagnussat, Mariana, Mónica Zapponi, Verónica Rodríguez Bauzada, et al. "Evaluation of Polymeric Coatings for MIC Problems During Oil and Gas Transportation." In LatinCORR 2023. AMPP, 2023. https://doi.org/10.5006/lac23-20428.

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Extended Abstract During oil and gas operations, pipelines are subjected to corrosion deterioration mechanisms, one of which is microbiologically influenced corrosion (MIC). The aim of this work was to analyze the behavior of different polymeric coatings in a continuous culture of sulfate-reducing bacteria (SRB) after 3 and 6 months of exposure. L80 API carbon steel samples with different coatings were placed in the bioreactor. At the end of the exposure periods, the samples were scraped with a sterile scalpel and cultured in Postgate B liquid medium to count biofilm-forming bacteria. Control
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de Paula, Renato M., Victor V. Keasler, and Timothy J. Tidwell. "Evaluation of Preservative Chemistries to Control Microbial Activity During Well Completion." In CORROSION 2015. NACE International, 2015. https://doi.org/10.5006/c2015-06069.

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Abstract Microbiologically-influenced corrosion (MIC) poses a serious concern to the production and the integrity of pipelines, vessels and tanks. High water content directly increases the risk for MIC as it results in an increase of the microbial load in the system. Compared to conventional production systems, unconventional fields are at a higher risk for premature MIC due to large volumes of water used to initially fracture the formation. Thus, microbial control during hydraulic fracturing is a critical step to prevent corrosion and maintain the integrity of the well. The use of traditional
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6

de Romero, Matilde F., Duque C. Zoilabet, Oladis T. de Rincón, Orlando Pérez, and Ismenia Araujo. "Hydrogen Permeation Study with Palladium in a Sulfate-Reducing Bacteria Culture." In CORROSION 2001. NACE International, 2001. https://doi.org/10.5006/c2001-01260.

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Abstract This study was undertaken to evaluate cathodic depolarization as the action mechanism triggered by sulfate-reducing bacterias (SRBs) in Microbiologically Induced Corrosion (MIC), using an inert substrate such as a 1-mm thick Palladium (Pd) strip with and without cathodic polarization, a H° permeation cell type by Devanathan and Stachurski, and the bacteria Desulfovibrio desulfuricans ssp. desulfuricans. The permeation tests were run using a de-aerated sterile culture medium inoculated with 10% D. desulfuricans at 108 cell/ml. Bacterial growth was evaluated by the serial dilution techn
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Lima, Maysa Ramos de, Ana Thereza da Cunha Uchoa, Ana Vitória de Sousa Melo, Maryanne Martim Furtado Lacerda, and Taynah de Almeida Melo. "SURGICAL EXERCISE OF INDUSTRIAL SILICONE IN A TRANSVESTI AFTER INFECTION: A CASE REPORT." In XXIV Congresso Brasileiro de Mastologia. Mastology, 2022. http://dx.doi.org/10.29289/259453942022v32s1076.

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Industrial liquid silicone (ILS) has been used clandestinely to modify body contours in Brazil. It is noted that both men (transvestites) and women (mainly sex workers) have been using ILS injections into the breasts. Its use can cause complications, such as infections, migration of the product to other areas of the body, deformities, siliconomas, tissue necrosis, and many other health problems. ILS is not sterile and is not intended to be applied to humans. In addition, a siliconoma is frequently found in silicone mastopathy and is probably related to the increase in breast cancer expansion,
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