Dissertations / Theses on the topic 'Sterilizace'

This diploma thesis is focused on studying of the effect of the dielectric barrier discharge (DBD) on eucaryotic microorganisms. Plasma sterilization is considered to be an alternative method to conventional sterilization processes. Contrary to standard decontamination methods it doesn´t stress exposed material by heat, pressure and chemicals. Plasma acts on eucaryotic and procaryotic systems by means of synergy of three inactivation mechanisms. They are various reactive species, UV radiation and heat. The Aspergillus niger has been chosen as a bio-indicator enabling to evaluate the effect of plasma assisted microbial inactivation. Plasma was generated in dielectric barrier discharge (DBD) at atmospheric pressure. Nitrogen and argon were used as working gases, paper and PET foil were used as carrying media. The influence of various working conditions on the sterilization effect was studied. Namely it was the influence of plasma exposition time, plasma power density, the type of operating gas and type of supporting medium. The effect of UV radiation in combination with temperature, temperature and direct plasma were studied separately. According to our results the efficiency of DBD increases with plasma power density, resp. plasma exposition time. When comparing sterilization efficiency of nitrogen and argon operating at the same conditions, the higher sterilization effect was observed in argon. The influence of the carrying medium on sterilization effectiveness was proved. It was caused by the different structure of surface. It was found out, that in our experimental setup the active species are probably the main inactivation mechanism. The influence of temperature on inactivation of microorganisms was negligible. The combination of UV radiation and temperature reached the decontamination level about 2 orders. The discharge parameters were studied by means of optical emmision spectroscopy. Scanning electron microscopy enabled to evaluate possible damage of exposed materials through DBD.

Presented master’s thesis is focused on the study of the elementary inactivation processes acting during sterilization of procaryotic systems in dielectric barrier discharge (DBD). Sterilization is an important biomedical and food-industry application and plasma sterilization is one of the methods, which are suitable for sterilization of heat and chemical sensitive materials. Biologically contaminated samples were treated in dielectric barrier discharge operated at atmospheric pressure. The discharge was generated in argon and in nitrogen. The plasma power density was 2725,93 mW cm–3 in argon and 2325,93 mW cm–3 in nitrogen. Gram positive bacteria Bacillus subtilis and gram negative bacteria Escherichia coli were used as a bioindicator. Bacteria were spread onto the surface of Whatman No.1 filtration paper. The influence of UV radiation, reactive species, heat and plasma discharge where the synergistic function of all of the agents was studied. Effects of UV radiation and temperature were studied separately. In order to separate the effect of UV radiation generated by DBD the quartz glass transmitting UV radiation was employed. During the plasma exposition selected samples were covered with the quartz window while other samples were directly exposed to the plasma. Covered samples were exposed to UV radiation and temperature (which cannot be eliminated), samples without quartz window were directly plasma exposed (treated). Results show that for covered samples the lower inactivation was reached than by the samples directly exposed to plasma. When studying the effect of temperature, the temperature between the DBD electrodes was measured by means of a thermocouple. Afterwards the samples were placed in an oven and exposed to the same temperature as was measured between the electrodes. By comparing the results of heat treated samples and plasma treated samples it can be assumed that the influence of the temperature during the sterilization process in DBD is very low. The discharge parameters were studied by means of the Optical Emission Spectroscopy. Plasma treated samples were assessed employing Scanning Electron Microscopy (SEM). Damage of Bacillus subtilis cell wall due to the effect of plasma was observed while no effect of plasma on the structure of filtration paper was detected.

The overall goal of the presented dissertation thesis was to study the sterilization efficiency of dielectric barrier discharge operated at atmospheric pressure. The fungi Aspergillus niger, gram-positive bacteria Bacillus subtilis and in some experiments also gram-negative bacteria Escherichia coli were used as a bio-indicator enabling to evaluate the effect of plasma assisted microbial inactivation. The samples of microorganism were placed on paper Whatman 1 or PET foil and exposed to plasma. The plasma was generated in argon, nitrogen, synthetic dry/humid air with frequency up to 10 kHz and plasma power density in the range of 1,2-2,9 W/cm3 (according to the process gas). The influence of process gas, plasma power density, plasma exposition time, type of microorganism and material of the substrate on the sterilization effect of dielectric barrier discharge was evaluated. Furthermore the contribution of each single mechanism (UV radiation, temperature and reactive species) to the sterilization effect of plasma and influence of gas humidity was evaluated. The DBD was analysed by means of optical emission spectroscopy, thermocouple was used to measure temperature during a sterilization process. In order to verify the mechanical damage of the microbial cell or the substrates during the plasma process the samples were studied by scanning electron microscopy. Generally, on the basis of experimental results, at increasing treatment times, the remaining number of spores (CFU) decreased. Similarly at increasing the plasma power input, the sterilization rate increased. When sterilising the spores of A. niger in plasma using different process gasses, the efficiency of plasma sterilization decreased as follows: argon, humid synthetic air, nitrogen and dry synthetic air. The results observed in argon plasma using different microorganism demonstrated that the sensitivity of vegetative cells resp. spores to DBD decreased as follows: A. niger spores, B. subtilis vegetative cells, E. coli vegetative cells and B. subtilis spores. Simultaneously results observed for sterilization of spores and vegetative cells of B. subtilis and A. niger demonstrated that the spores are generally more resistant to plasma than are the corresponding vegetative cells. Combining the results of contribution of each single mechanism, optical emission spectroscopy and inactivation characteristic it was found out that the reactive species significantly contribute to the plasma sterilization in all process gasses. Furthermore the inactivation process can be partly assisted by UV radiation and also the temperature can contribute in limited extent to inactivation process in some gasses. The contribution of UV radiation to the plasma sterilization decreased as follows: nitrogen, argon, dry syntetic air and humid syntetic air. Moreover it was found out that the contribution of each single mechanism can be species dependent, this is due to the different response of microorganism to the unfavorable external conditions. SEM analysis of the substrates prooved the etching actions of the plasma generated in all process gasses on the surface of the PET foil. The several minute plasma exposition of the PET foil resulted in the occurence of the „hole corrosion“ on the PET surface. Contrary to these there were no visible changes observed in the paper structure.

This master´s thesis is focused on the utilization of the sterilization effects of low temperature plasma towards the bacterial microorganisms that occur mainly on the human skin. The plasma sterilization process is fast efficient, non-toxic, environmentally friendly, cost-effective and safe for the operating staff as well as for the patient. Another advantage of using low temperature plasma is to support cell proliferation and wound healing. By combining these advantages, an effective method can be obtained, which would sterilize the wounds sparingly with regard to the surrounding healthy tissue and support the regeneration of the damaged tissue at the same time. In the experimental part, gram positive and gram negative bacteria were used to prove the sterilization effects with respect to different cell wall structure. Staphylococcus epidermidis and Propionibacterium acnes, which cause purulent skin inflammations, were used as gram-positive microorganisms. Serratia marcescens and Escherichia coli were selected from gram positive bacteria. These model organisms were inoculated at various concentrations on culture broths and treated by plasma at a distance of 1 mm from the agar surface. The microwave discharge was generated in argon at a power of 9 W, a gas flow rate of 5 l / min and water cooling to avoid thermal effects on the treated surface. Subsequently, model skin cells of HaCaT were exposed to low temperature plasma and tested for plasma cytotoxicity to demonstrate its healing effects. The obtained results make it possible to state that the sterilizing effects of low-temperature plasma in all tested gram-positive and gram-negative bacterial strains are verified in this work. Finally, tests were demonstrated using a suitable method of the treatment on human skin cells, where the safety and usefulness of the tested low-temperature plasma was demonstrated when applied to shorten the healing process.

The master’s thesis deals with finding, measurement and evaluation of suitable physical parameters for quality analysis of saturated steam sterilization according to ČSN EN 285 + A2, which is the first step for the replacement of outdated processes of the analysis by modern automation systems. The other part of the project contains description of physical sterilization, saturated steam, methods and sensors for the monitoring of steam sterilization and the measurement and evaluation of these issues.

This paper deals with proposal of new continual system for quality measurement of sterilization steam. At the begining the process of sterilization is introduced. Then the concept of saturated streams, parameters for sterilization and sensors for measurement are described. Then the current process of measurement of steam quality is described and in the last part the proposal of the new system is introduced. After the draft was the measurement data, analysis and evalution.

Diplomová práce se zabývá měřením, analýzou a následným nalezením vhodných fyzikálních parametrů pro analýzu kvality syté sterilizační páry. Tato práce si klade za cíl určit, kritické parametry systému vyvíječe páry pro provoz dle norem ČSN. Dílčí částí projektu je popis fyzikální sterilizace, syté páry, metod a senzorů pro kontrolu sterilizační páry.

The main subject of this diploma thesis is the study of the effect of humidity on the total sterilization effect of the dielectric barrier discharge. Sterilization is a process which can eliminate all forms of life. The plasma sterilization is one of the methods that are suitable for sterilization of temperature and chemical sensitive materials. This sterilization method was proved to be effective on the wide spectrum of procaryotic and eucaryotic microorganisms. Basically, the main inactivation factors for cells exposed to plasma are heat, UV radiation and various reactive species. Dielectric barrier discharge (DBD) operating at atmospheric pressure was used for the sterilization of the samples. The discharge was generated in dry air and in humid air. The plasma power densities were 2 160 mW.cm-3, 2 279 mW.cm-3 and 2 760 mW.cm-3 (dry air) or 2 326 mW.cm-3 and 2 850 mW.cm-3 (humid air). Humidity of air was achieved using a wash bottle filled with water through which air flowed into the DBD reactor. Fungi spores of Aspergillus niger were used as model microorganisms. Whatman paper No. 1 was used as the carrying medium. When comparing sterilization efficiency of humid and dry air operating at the same conditions, the higher sterilization effect was observed in humid air. The sterilization effect of the DBD generated in air was compared with results obtained during plasma generation in argon and nitrogen. At the same conditions, the highest sterilization effect was observed in argon, followed by humid air, nitrogen and dry air. It was found out that in our experimental setup the active species are probably the main inactivation mechanism. The influence of temperature on the inactivation of microorganisms was completely negligible. The discharge parameters were studied by means of the optical emission spectroscopy (OES). Plasma treated samples were analyzed employing scanning electron microscopy (SEM). Damage of the microorganisms due to the effect of plasma as well as plasma effect on the structure of the carrying medium was evaluated.

Whey proteins are mainly a group of small globular proteins. Their structures can be modified by physical, chemical and other means to improve their functionality. The objectives of this study were to investigate the effect of radiation on protein-protein interaction, microstructure, and microbiological properties of whey protein-water solutions. Whey protein isolate (WPI) solutions (27-36% protein) were treated with different dosages (10-35 KGy) of gamma radiation. The protein solutions were analyzed for viscosity, turbidity, soluble nitrogen, total plate count, and yeast and mold counts. The interactions between whey proteins were also analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and scanning electron microscopy (SEM). The viscosity of protein solution (27%, w/w) was increased from 2.19 for the control to 4.78 mPa*s for the sample treated at 25 KGy, respectively, and viscosity also increased during storage at 23°C. The soluble nitrogen (10%, w/w) was decreased from 100% to 54.7% for control and the sample treated at 35 KGy. The effects of gamma radiation and storage time on viscosity of whey protein solutions were significant (p

58 Název diplomové práce v anglickém jazyce: Legislation on abortion, sterilization and artificial insemination Resumé: This work deals with three areas of medical law, such as abortion, sterilization and assisted reproduction. These all three areas are characterized by a common role of women (patient) and the child to be born or not. Thesis describes the current legislation in comparison with the draft Law on specific health services, which was in November approved by the Government and now it will address to Parliament. The issue of abortion, as an intervention, which terminates pregnancy, the thesis set out the general interpretation, explanation of terminology, outlining the views of opponents and supporters of abortions and history of these treatments. It is also described current legislation and made its assessment in terms of application experience. There are pointed out the changes that will bring the new law. There is an example of case law. Subsequently, it is made an international comparison. Chapter relating to sterilization as a treatment, which ultimately deprives a woman of fertility, contains the first general outline of the issues and provides some historical context The work describes the current legislation in the Czech Republic. Within each of the relevant legislation is provided a...

This M.A. Thesis brings a survey about sexual sterlizations on Roma in the Czechoslovak area during the period from the first Czechoslovak Republic (1918 - 1939), over the Nazi's era (1939 - 1945), postwar and communist era (1945 - 1989) and post-communist era to the partition of the Czechoslovakia in 1993. Thesis also includes and discusses the process of investigation and admiting some sterilizations as illegal and refer to the role of the governmental and non-governmental institutions in this process.

Název diplomové práce v anglickém jazyce: Legal aspects of abortion, sterilization and assisted reproduction Summary: The thesis deals with the three topics from the field of medical law, particularly abortion, sterilization and assisted reproduction. The issue of giving birth is the key here. The new Special Medical Services Act regulating, inter alia, some aspects of sterilization and assisted reproduction came into effect in April 2012. The previous regulation of these matters was outdated and some legal issues were not tackled at all. The first part of the thesis focuses on legal regulation of abortion, which is contained in the act from 1986 and in the implementing legislation. Especially the conditions of intervention itself, payments and intervention on juveniles (under 18) and foreigners in the Czech Republic are tackled. The regulation of other countries is mentioned and compared as well. The second part of the thesis deals with the legal regulation of sterilization, conditions of sterilization and contraindication as well. The specific problem of illegal sterilizations of gypsy women in the second half of the twentieth century is pointed out. These disputes were present even before the European Court for Human Rights in Strasbourg. The methods of assisted reproduction, as a modern way of resolving...

Legal aspects of gender reassignment Abstract Thesis deals with legal aspects of gender reassignment, especially with legal conditions of gender reassignment witch are required by the Czech law. Furthermore, the thesis provides a general introduction to the issue of transsexuality, deals with the etiology of transsexuality, and defines its two fundamental types, namely the MtF and the FtM transsexuality. This thesis also recalls the position of transgender people in society, as transgender people continuously face various forms of discrimination. In particular, the text of the thesis is focused on the legal analysis of the issue of gender reassignment. The thesis deals with all legal regulations that contain provisions concerning gender reassignment. In this manner, it deals with all relevant legal provisions included in the Czech Civil Code, in the Act on Specific Health Services, and in the Registry Act. The thesis also deals with the possible future legal regulation of gender reassignment using the de lege ferenda considerations. This bill could adapt the Czech legal regulation of gender reassignment to the case-law of The European Court of Human Rights as well as the legal standarts in other european countries. In the thesis, there is also the whole process of gender reassignment including both the...

Sturgeons are commonly known as living fossils or ancient giants that diverged from ancient pre-Jurassic teleost lineage approximately ~300 million years ago (Mya). Sturgeons' 85% species are listed as critically endangered in the International Union for Conservation of Nature (IUCN). Sturgeons' reproductive traits such as delay in sexual maturation and periodic interrupted spawning cycles make their rehabilitation more difficult. However, among sturgeon species, the sterlet (Acipenser ruthenus) has shortest sexual maturation period. Therefore, it can be used as a host in surrogate production in sturgeons. Dnd1 was discovered as germ-plasm specific maternal RNA that exclusively expresses in vertebrate germ-line. Various studies have confirmed that dnd1 protein is essential for Primordial Germ Cells (PGCs) migration; and disruption of the PGCs migration affects fish fertility. Dnd1 deficient PGCs in zebrafish transdifferentiate into somatic cells. Previously our colleagues used morpholino oligonucleotide to knock down dnd1 in sterlet to produce germ cell free host for surrogate production. CRISPR/Cas9, a cutting-edge genome editing technology is being used in different research fields; here we thus aimed to harness the power of aforementioned technology to knock out dnd1 in sterlet. No or less number of PGCs were observed in CRISPR/Cas9 injected embryos as compared to control group injected with FITC-dextran only in order to label PGCs. Furthermore, we compared three different sterilization techniques viz., CRISPR/Cas9 and morpholino oligonucleotide (MO) targeting dnd1 and ultraviolet irradiation to eliminate PGCs in sterlet. Our data showed higher hatching and survival rates in CRISPR/Cas9, UV irradiation, and MO knockdown groups, respectively. Interestingly, some embryos treated with CRISPR/Cas9 displayed malformations. We presume that malformations were due to off-target effects and/or due to double injections i.e., injection of CRISPR/Cas9 at animal pole to knock-out the dnd1 and FITC-dextran at vegetal pole. Taking advantages of Iron Oxide nanoparticles (IONs) applications in various burgeoning research fields, we opted to use them to label PGCs in sturgeons. We injected IONs combined with FITC-dextran into vegetal pole of sturgeon embryos, and have successfully labelled the PGCs. Injection of IONs in sturgeons did not affect hatching and survival rates of embryos. Interestingly at 5 dpf, significantly less number of FITC-dextran labelled PGCs in FITC-dextran/IONs injected group were observed when compared with PGCs that were labelled with FITC-dextran only. Less number of PGCs in IONs injected group presumably could be because of interference posed by IONs to PGCs during the course of their migration. This is first study of its kind where germ cells of any species have been labelled by using nanoparticles. In conclusions, this thesis provides information regarding role of Dnd1 protein as potential germ-cell molecular marker in various fish species, and its use for conservation of fish species. Dnd1 knockout sterlet can be potentially used as sterile host for surrogate production in sturgeons. Moreover, labelling of PGCs in sturgeons by using IONs can thus open new avenues to study interactions of nanoparticles with cells that will ultimately help in hyperthermia where cells/tissues are exposed to electromagnetic field increasing temperatures to activate their death. After insertion of IONs to PGC in sturgeon embryo, it could be possible to isolate PGC using a magnetic field or to apply hyperthermia for host sterilization purpose.

The aim of the work: The aim of this thesis is to provide detailed information about organization and operation of today's operating rooms from nurse´s point of view. Operational treatment requires high demands on technical and material equipment, special staff training, perfect adherence to asepse, antisepsis, sterility as well as work organization. The thesis should also introduce the most urgent problems of the current situation of the operating rooms management. The most recent discoveries and proven methods are summarized in a simple and comprehensible form that will serve as a guide for perioperative nurses. Method: Qualitative research methods in the form of a semi-structured interview have been used. This thesis will use the qualitative survey method in the form of a semistructured interview along with snowball sampling. The research consists of respondents with finished specialization in instrumentation for the operating room who are still active in the given field. Current status: The organization and operation room management consists of two functional models. These are so called "sideline sterilization" versus central operating rooms. Both ways have their advantages and disadvantages related to workload, general nursing education, and operating rooms management. However, the least preferred option is a combination of both perioperative operations. Results: The results of the research focused on the centralization and operating rooms management show that "sideline sterilization" is considered an obsolete model of tool sterilization for operating rooms. The results also show that the model of central operating rooms with the combination of central sterilization is more modern and efficient both in terms of expenses and effort, not just for nurses but for all healthcare professionals. Conclusion: In accordance with the aim of this work the issue of centralization of operating rooms is pointed out, considering both the construction with the necessity to follow all known procedures in compliance with aseptic conditions, as well as the need of good management or nurse education. The results can be used for further research and as a further source of information for non-medical fields, mainly perioperative care.

The induction of germ-line chimerism is an expanding focus of fisheries research. This technique is having a potential to enhance the production of gametes of species that are commercially valuable, endangered, species with problematic reproduction, using a more common or easily available species or species adapted to artificial reproduction as a surrogate host. The main goal of this technology is to establish a small-bodied surrogate broodstock producing functional donor gametes based on germ cell transplantation. Extent preliminary experiments, including documentation of donor/host embryonic and larval development, characterization of germ cells enriched by documentation of their migratory activities, sterilization of the host, isolation and cryopreservation of donor germ cells, are key factors for launching this biotechnology. All these crucial points were the main objective of the present work. The whole thesis provided the focus on two different fish species. First, our commercially valuable fish, the tench, where we would like to apply our current knowledge to create a germ-line chimera within cyprinids by transplantation of tench germ cells to smaller and faster-reproducing fish species as white cloud mountain minnow. Secondly we focused on the endangered species (listed in IUCN Red List) of large body size with long reproductive cycle, the sturgeons. In this case, we have chosen sterlet as a host, providing an advantage of shorter generation interval and smaller body size, to produce gametes of donor, a critically endangered species of large body size with long reproductive cycle, such as beluga. This innovative technology could result in collection of sperm and eggs in shorter time from small-bodied host. In tench we firstly focused on embryonic and larval development documentation together with description of origin and migration pathways of primordial germ cells (PGCs). PGCs represent a powerful tool for creation a germ-line chimera within fish species because they transmit genetic information to the next generation (Linhartova et al., 2014a). Secondly, we reported a practical technique for isolation and cryopreservation of early stages of germ cells (GC), including spermatogonia (SG) and spermatocytes (Linhartova et al., 2014b). In case of sturgeons, Saito et al. (2014) firstly described the origin and migration patterns of sturgeon PGCs deposited at the vegetal pole of the egg similar to that in anurans. Secondly, Psenicka et al. (2015) reported isolation and cryopreservation of female and male GC, SG from testes and OG and pre-vitellogenic oocytes from ovary, of 2-4-year old Siberian sturgeon. Moreover the isolated GC were transplanted into host (sterlet) and process of transplantation resulted in successful colonization of sterlet genital ridge. The potential host for germ-cell tranplantation, sterlet, was sterilized by knock-down of germ cell specific gene, the dead end gene, by the morpholino antisense oligonucleotide (MO) agent (dnd-MO). These results reported the first known and functional method of sturgeon sterilization (Linhartova et al., manuscript). We provided important information on morphology and ultrastructure of beluga spermatozoa structure by scanning and transmission electron microscopy to increase knowledge of evolutionary and taxonomic relationships among sturgeons (Linhartova et al., 2013). Finally, this thesis presents several studies with differing focus of research but with one target goal to induce germ-line chimerism in fish. All these results are prerequisite of future application and development of surrogate production in these species.

The degree work is concentrated on basic characterization of nosocomial infections, on the prevention thereof and on the observance of antiepidemical measures in the sphere of stomatology. Although these infections were largely related to the mortality and morbidity in health service establishments in the past, their occurrence goes down significantly these days. The adoption and observance of fundamental hygienic principles and rules was of decisive importance in this sphare. The neglect of these principles can endanger the health and life of the patient seriously end bring about the increase of costs for further therapy of the patient. It is therefore necessary even at present time ot pay great attention to these diseases and to the prevention thereof. The research part of the work is devoted to the evaluation of preventive measures connected with nosocomial infections in stomatological practice. The literature search in the theoretical part follows therefore this orientation too. The theoretical part deals at first with the most frequent agents of nosocomial infections, that can be encountered. The attention is concentrated on brief characterization of these diseases, their sources and on the process of spreading. Then, the basic classification of these diseases and the concrete methods of prevention are mentioned. In the sphere of stomatology, the work resumes the basic historical relations and identifies particular sectors of this discipline. In conneciton with nosocomial infections, their impact upon the stomatology as well as the need of protection against these infections are discussed. It means epidemiological measures, importance of hand hygiene, necessity of sterilization, desinfection and of cleaning. The aim of the work is to point out the importance of prevention as to the origin and spreading of these infections and the necessity to observe the epidemical principles at the work in dental surgeries. The performed investigation resumes established facts about the observance of hygienic and epidemiological regimen and that not only as to the general judgement on problems, but in consideration of local differences, of differences in the duration of practice carried out by individual stomatologists and in relation to the frequency of conducted hygienic inspections as well. Problematic areas as to the nosocomial infections and as to the observance of decreed provisions by stomatologists are defined here. It refers also to the influence of the locality and to the frequency of inspections directed to the observance of these hygienic rules by physicians.

This diploma thesis deals mainly with utilization of plasma in technological aplications. The introductory part is apllied to the theoretical description of plasma generated by the surfatron. This launcher works with the frequency of 2,45 GHz and it is able to excite the surface wave, that sustains plasma column in a quartz tube. Plasma generated by surfatron was used for modification of surface properties of polyethylen to change its surface energy. By changing of surface energy it was reached of higher hydrophilicity. Plasma generated by surfatron was diagnosed in detail during experiments in a continual regime as well as in a pulse one with a help of a single {--} probe and a double {--} probe Langmuir measurement. In this diploma thesis there is discussed influence of pressure, power and other experimental options.

The orchid family covers 90 percent of the rarest plants under protection all over the world. The ecological and economic value of orchids has aroused public awareness. Since vegatitve propagation methods are available, development of in vitro methods are essential for conservation and commercialization of plants. Purposes work was elaboration progress of vegetative multiplication for tropical orchid Macodes petola in conditions in vitro. At research were proof 4 methods, which distinguish in using desinfection, in operating time and in using different nutritive mediums.

Diploma thesis "Nazi ideology in relation to mentally ill people" focuses on starting points and ideas of this ideology, which in its consequences led to social exclusion and subsequent systematic extermination of people with a certain handicap. Although the thesis is limited in time from 1933 - 1945, it also follows development line of selected scientific disciplines, which were significantly reflected in the Nazi ideology. Primarily it concerns historical science, medical disciplines, genetics, racial hygiene, eugenic origins and other related disciplines. The thesis also takes into account the economic situation of that time with its many social problems which arose as a result of the new geopolitical arrangement after the World War I and the changes in the social world of that period and it also captures the process of changes in the social status of mentally ill people in the course of history. Last but not least, it describes the practical consequences of these changes, which were "forced sterilization" and "euthanasia" of both children and adults. The Nazi ideology is viewed in this regard from three perspectives: legal, theoretical with a broader historical context and on the practical level in the form of the consequences of the two previous perspectives. Key words: nazi ideology, mental...