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Journal articles on the topic 'Sterilizing agent'

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1

Amantayev, Nurbolat Gabdullaevich, Marina Mikhailovna Silantyeva, and Vadim Tagirovich Khassanov. "Selecting sterilization conditions for the explants of different potato varieties to be in vitro introduced into the culture." Bulletin of the Karaganda University. “Biology, medicine, geography Series” 111, no. 3 (2023): 16–24. http://dx.doi.org/10.31489/2023bmg3/16-24.

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An explant is a fragment of plant tissue or organ that is incubated on its own or used to produce a primary callus. The introduction of any plant’s tissues into the culture in vitro begins with the selection of an uninfected viable explant. The authors presented the results of the effect of various sterilizing agents as well as the treatment timing on the viability of plant explants during microclonal propagation of potatoes of the following varieties: Aladin, Gala, Nevsky, Udacha and Kostanay Novosti. The meristem sprouts from potato tubers were used as parent material. The following commerci
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2

Hashim, Saiyidah Nafisah, Siti Zulaiha Ghazali, Norrizah Jaafar Sidik, Tay Chia-Chay, and Azani Saleh. "Surface sterilization method for reducing contamination of Clinacanthus nutans nodal explants intended for in-vitro culture." E3S Web of Conferences 306 (2021): 01004. http://dx.doi.org/10.1051/e3sconf/202130601004.

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Surface sterilization is a vital step in preparation of healthy and viable explants in tissue culture. Most surface contaminants can be eliminated by surface sterilization with a suitable sterilizing agent. The study aimed to present an effective disinfection method for Clinacanthus nutans shoot regeneration using nodal segments. A total of four different sterilization approaches were conducted by treating nodal explants with various concentrations of sterilizing agent. Sterilizing agents used were Rhizophora apiculata Pyroligneous acid (PA), sodium hypochlorite (Clorox) thiophanate-methyl (fu
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3

Belete, Yonas Zeslase, and Hagos Kalu. "Protocol Optimization for Surface Sterilization of Sugarcane Variety (B-52/298)." International Letters of Chemistry, Physics and Astronomy 48 (March 2015): 50–60. http://dx.doi.org/10.18052/www.scipress.com/ilcpa.48.50.

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The aim of this study was to optimize conditions for surface sterilization of ex-plants of B-52/298 sugarcane cultivar variety. Experiment was carried out to determine the optimum concentrations of surface sterilizing agents and at what time they optimally sterilize. This experiment was conducted at MIT Plant Tissue Culture and Micro-propagation Laboratory Center, situated in Mekelle City. Three chemisterilants (Sodium Hypochlorite, Mercuric Chloride and Ethanol) were taken for the investigation. Three of the sterilizing agents were used in different concentrations at various time intervals an
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4

Belete, Yonas Zeslase, and Hagos Kalu. "Protocol Optimization for Surface Sterilization of Sugarcane Variety (B-52/298)." International Letters of Chemistry, Physics and Astronomy 48 (March 25, 2015): 50–60. http://dx.doi.org/10.56431/p-ta6564.

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The aim of this study was to optimize conditions for surface sterilization of ex-plants of B-52/298 sugarcane cultivar variety. Experiment was carried out to determine the optimum concentrations of surface sterilizing agents and at what time they optimally sterilize. This experiment was conducted at MIT Plant Tissue Culture and Micro-propagation Laboratory Center, situated in Mekelle City. Three chemisterilants (Sodium Hypochlorite, Mercuric Chloride and Ethanol) were taken for the investigation. Three of the sterilizing agents were used in different concentrations at various time intervals an
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5

Rakovsky, Slavcho, and Gennady Zaikov. "Application of Ozone in Medicine." Chemistry & Chemical Technology 3, no. 3 (2009): 237–48. http://dx.doi.org/10.23939/chcht03.03.237.

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This review deals with the application of ozone in medicine, its effects on the human organism and its use as a therapeutic approach and sterilizing agent. A particular attention is paid to the therapeutic properties, therapeutic dosage and scope of application. Some mechanisms of the ozone effect at exposure on different organs and systems in human body are also considered. Ozone toxicity is reviewed. The ozone use as a sterilizing agent in the pharmaceutical industry and cosmetics, as well as in the food processing industry is discussed.
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Neshumaeva, N. A., and M. A. Timina. "Selection of methods for sterilization of C. purpurea sclerotia for isolation of rye biosecurity agents." IOP Conference Series: Earth and Environmental Science 839, no. 4 (2021): 042010. http://dx.doi.org/10.1088/1755-1315/839/4/042010.

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Abstract Winter rye is a traditionally cultivated grain crop that is susceptible to ergot, a serious disease of the Poaceae family, caused by C. purpurea. Along with agrotechnical, organizational, and economic methods, biological control of ergot in grain crops is one of the ways to combat this disease. To isolate C. purpurea hyperparasites in a pure culture, the optimal sterilization options were selected, which were to exclude the infection of ergot sclerotia with saprophytic microflora. Before sterilization, C. purpurea were additionally washed in the running water with the addition of SAS
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7

B.M., Bhyravi, Athani S.I., D. P. Prakasha, et al. "Effect of sterilizing agent and seed treatment method on In vitro seed germination of guava cv. Arka Kiran." Ecology, Environment and Conservation 30, no. 02 (2024): 588–91. http://dx.doi.org/10.53550/eec.2024.v30i02.028.

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The in vitro germination of seeds of guava cv. Arka Kiran was mainly affected by contamination and seed coat dormancy. For successful in vitro germination of guava seeds, contamination control and breaking of seed dormancy are the two main steps one should fallow. An experiment on “Effect of sterilizing agent and seed treatment method on in vitro seed germination of guava cv. Arka Kiran” was carried out at College of Horticulture, Sirsi, Uttar Kannada, Karnataka during 2021-2023. The study revealed significant influence of different sterilizing agents and seed treatment method on contamination
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8

Markova, Marina, and Elena Somova. "Regeneration capacity of Cerasus fruticosa and Prunus domestica into the in vitro culture." Agrarian Bulletin of the 209, no. 06 (2021): 43–52. http://dx.doi.org/10.32417/1997-4868-2021-209-06-43-52.

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Abstract. The aim of these studies was to introduce into the in vitro culture the steppe cherry (Cerasus fruticosa) variety Shchedraya and the domestic plum (Prunus domestica) variety Sineokaya for subsequent micropropagation. Methods. Optimal conditions for obtaining viable explants, such as sterilizing agent and initiation time, have been investigated. The suitability of various nutrient media for in vitro cultivation of these cultures has also been tested. As a result of the experiments, it was revealed that the most effective sterilizing agents were 38 % perhydrol (control) and 6% chlorhex
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9

NAGASUPRIYA, T. VEERA, K. VENKATA LAXMI, P. SAIDAIAH, VEENA JOSHI, and M. GOPALA KRISHNA. "EFFECT OF DIFFERENT SURFACE STERILIZATION METHODS AND DIFFERENT PLANT GROWTH REGULATORS ON CULTURE ESTABLISHMENT FROM POMEGRANATE NODAL EXPLANTS OF cv. BHAGWA." Asian Journal of Microbiology, Biotechnology & Environmental Sciences 26, no. 04 (2024): 486–91. https://doi.org/10.53550/ajmbes.2024.v26i04.007.

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The present investigation was conducted to know the effect of different surface sterilization methods and different plant growth regulators on culture establishment from Pomegranate nodal explants of cv. Bhagwa with two factors: Sterilizing agents(S): 0.1% Mercuric chloride (HgCl2) and 1% Sodium hypochlorite (NaOCl) each at two different timing intervals i.e., 5 min and 10 min and growth regulators(G) G1: BAP 2 mg L-1, G2: Kinetin 2 mg L-1, G3: 2-ip 2 mg L-1, G4: zeatin 2 mg L-1). The results revealed that highest percentage of uncontamination was recorded in S2: 0.1% HgCl2 for 10min (93.33%)
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10

MARKOVA, M. G., and E. N. SOMOVA. "INITIATION OF HORTICULTURAL CROP EXPLANTS IN VITRO." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 4 (2022): 71–81. http://dx.doi.org/10.26897/0021-342x-2022-4-71-81.

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The stage of introduction into sterile culture plays an important role in clonal micropropagation. The purpose of these studies was to find the optimal sterilizing agent at the stage of introducing blue honeysuckle, red raspberry, garden strawberry, steppe cherry, house plum into in vitro culture, as well as the optimal nutrient medium at this stage for blue honeysuckle and red raspberry. As sterilizing agents with optimal exposure, the following were used: 48% ethyl alcohol (control) 8–10 min., 33% perhydrol 6–8 min., 6% chlorhexidine 8–10 min., 0.1% sublimate 1–2 min., 10.0% Domestos 6–7 min
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11

Mazzola, Priscila Gava, Angela Faustino Jozala, Letícia Célia de Lencastre Novaes, Patricia Moriel, and Thereza Christina Vessoni Penna. "Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents." Brazilian Journal of Pharmaceutical Sciences 45, no. 2 (2009): 241–48. http://dx.doi.org/10.1590/s1984-82502009000200008.

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Due to the growing number of outbreaks of infection in hospital and nurseries, it becomes essential to set up a sanitation program that indicates that the appropriate chemical agent was chosen for application in the most effective way. Validating the effectiveness of decontamination and disinfection is an important and often challenging task. In order to study and compare the behavior of selected microorganisms, they were submitted to minimal inhibitory concentration (MIC). The MIC intervals, which reduced bacteria populations over 6 log10, were: 59 to 156 mg/L of quaternary ammonium compounds
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12

Lal, Manmohan, Mahital Jamwal, Parshant Bakshi, et al. "Establishing surface sterilization protocol for clonal apple rootstock MM106." Journal of Applied and Natural Science 15, no. 4 (2023): 1654–59. http://dx.doi.org/10.31018/jans.v15i4.5147.

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Surface sterilization is crucial in preparing viable and uncontaminated explants for tissue culture. Utilizing the right sterilizing agent in this procedure can efficiently eliminate the majority of surface contaminants. The present study aimed to establish the best sterilization procedure for clonal apple rootstock MM106 using shoot tips and nodal segments. Two important sterilizing agents, mercuric chloride (ranging from 0.05% to 0.3%) for various durations (2 to 6 minutes) and sodium hypochlorite (ranging from 3.0% to 12%) for different durations (10 to 30 minutes), were employed. The resul
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13

Widjaksana, Widyastuti, Enny Lestari, Maskur Maskur, and Sudarsih Sudarsih. "Autosterilization Effect of Gamma Radiation in Non-sterile Radiopharmaceuticals." 3BIO: Journal of Biological Science, Technology and Management 4, no. 1 (2022): 19–25. http://dx.doi.org/10.5614/3bio.2022.4.1.3.

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Gamma radiation is commonly used in sterilizing various products because of its microbial-killing property. Several radiopharmaceuticals are processed aseptically, which requires a well-managed GMP-based facility. This study aims to ensure that radiopharmaceutical can behave as an auto sterilizing agent since it contains radionuclide that emits gamma radiation. Sm-153-ethylenediaminetetramethylenephosphonate (Sm-153-EDTMP) and I-131-Hippuran were used as models in this study, in which various radioactivities of these products were added into non-sterile vials in the non-aseptic working area an
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14

Mazzola, Priscila Gava, Angela Faustino Jozala, Letícia Célia de Lencastre Novaes, Patricia Moriel, and Thereza Christina Vessoni Penna. "Choice of sterilizing/disinfecting agent: determination of the Decimal ReductionTime (D-Value)." Brazilian Journal of Pharmaceutical Sciences 45, no. 4 (2009): 701–8. http://dx.doi.org/10.1590/s1984-82502009000400013.

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Efforts to diminish the transmission of infections include programs in which disinfectants play a crucial role. Hospital surfaces and medical devices are potential sources of cross contamination, and each instrument, surface or area in a health care unit can be responsible for spread of infection. The decimal reduction time was used to study and compare the behavior of selected strains of microorganisms. The highest D-values for various bacteria were obtained for the following solutions: (i) 0.1% sodium dichloroisocyanurate (pH 7.0) - E. coli and A. calcoaceticus (D = 5.9 min); (ii) sodium hyp
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15

Soto, Artur Ferronato, Rodrigo Alex Arthur, Myriam Pereira Kapczinski, Marcelo Lazzaron Lamers, and Cristiane Machado Mengatto. "Keratinocyte Cytotoxicity of Peracetic Acid Used as Sterilizing Agent for Implant Scaffolds." Annals of Plastic Surgery 83, no. 1 (2019): 99–103. http://dx.doi.org/10.1097/sap.0000000000001967.

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16

Bakuleva, N. P., N. M. Anuchina, M. V. Zelivyanskaya, et al. "Preclinical Trials of Dioxidine as a Sterilizing Agent for Heart Valve Bioprostheses." Biomedical Engineering 49, no. 3 (2015): 164–67. http://dx.doi.org/10.1007/s10527-015-9521-y.

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17

Tasneen, Rokeya, Kathy Williams, Opokua Amoabeng, et al. "Contribution of the Nitroimidazoles PA-824 and TBA-354 to the Activity of Novel Regimens in Murine Models of Tuberculosis." Antimicrobial Agents and Chemotherapy 59, no. 1 (2014): 129–35. http://dx.doi.org/10.1128/aac.03822-14.

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ABSTRACTNew regimens based on two or more novel agents are sought in order to shorten or simplify the treatment of both drug-susceptible and drug-resistant forms of tuberculosis. PA-824 is a nitroimidazo-oxazine now in phase II trials and has shown significant early bactericidal activity alone and in combination with the newly approved agent bedaquiline or with pyrazinamide with or without moxifloxacin. While the development of PA-824 continues, a potential next-generation derivative, TBA-354, has been discovered to havein vitropotency superior to that of PA-824 and greater metabolic stability
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18

Jahn, Beate, Gaby Sroczynski, Martin Bicher, et al. "Targeted COVID-19 Vaccination (TAV-COVID) Considering Limited Vaccination Capacities—An Agent-Based Modeling Evaluation." Vaccines 9, no. 5 (2021): 434. http://dx.doi.org/10.3390/vaccines9050434.

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(1) Background: The Austrian supply of COVID-19 vaccine is limited for now. We aim to provide evidence-based guidance to the authorities in order to minimize COVID-19-related hospitalizations and deaths in Austria. (2) Methods: We used a dynamic agent-based population model to compare different vaccination strategies targeted to the elderly (65 ≥ years), middle aged (45–64 years), younger (15–44 years), vulnerable (risk of severe disease due to comorbidities), and healthcare workers (HCW). First, outcomes were optimized for an initially available vaccine batch for 200,000 individuals. Second,
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19

Loraine, Jessica, Feifei Pu, Obolbek Turapov, and Galina V. Mukamolova. "Development of anIn VitroAssay for Detection of Drug-Induced Resuscitation-Promoting-Factor-Dependent Mycobacteria." Antimicrobial Agents and Chemotherapy 60, no. 10 (2016): 6227–33. http://dx.doi.org/10.1128/aac.00518-16.

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ABSTRACTTuberculosis is a major infectious disease that requires prolonged chemotherapy with a combination of four drugs. Here we present data suggesting that treatment ofMycobacterium tuberculosis, the causative agent of tuberculosis, andMycobacterium smegmatis, a model organism widely used for the screening of antituberculosis agents, with first-line drugs resulted in the generation of substantial populations that could be recovered only by the addition of a culture supernatant from growing mycobacteria. These bacilli failed to grow in standard media, resulting in significant underestimation
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20

Yoganarasimha, Suyog, William R. Trahan, Al M. Best, et al. "Peracetic Acid: A Practical Agent for Sterilizing Heat-Labile Polymeric Tissue-Engineering Scaffolds." Tissue Engineering Part C: Methods 20, no. 9 (2014): 714–23. http://dx.doi.org/10.1089/ten.tec.2013.0624.

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21

Adachi, Takahisa, Masaki Kawamura, Atsushi Nakanishi, et al. "The effectiveness of AMTEC QC-70: New sterilizing agent for dialysate supply systems." Journal of Japanese Society for Dialysis Therapy 23, no. 12 (1990): 1377–81. http://dx.doi.org/10.4009/jsdt1985.23.1377.

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Zhao, Yantao, Jun Yan, Yang Xia, Feimin Zhang, and Ning Gu. "Enhanced Osteogenesis of Polyvinylpyrrolidone-Iodine as a Sterilizing Agent for Preservation of Allografts." Journal of Biomaterials and Tissue Engineering 5, no. 6 (2015): 465–71. http://dx.doi.org/10.1166/jbt.2015.1336.

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23

Sulaiman, Helmi, Shabrina Yasmin Ramadhani, Kresna Fajri Wicaksana, et al. "Explant Sterilization of Sandalwood (Santalum Album) and Teak (Tectona Grandis) Micropropagation with Commercial Disinfectant." BIODIVERS - BIOTROP Science Magazine 2, no. 2 (2023): 56–65. http://dx.doi.org/10.56060/bdv.2023.2.2.2097.

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Sandalwood (Santalum album) and Teak (Tectona grandis) are essential wood sources for furniture. Sustainable micropropagation methods can benefit wood availability. This study evaluates NaOCl and Povidone-iodine effects, immersion duration, and concentrations on teak and sandalwood micropropagation. Teak is treated with 10% & 15% NaOCl and 10% & 15% Povidone-iodine. No significant difference was observed between teak treatments; however, povidone-iodine shows potential as an effective teak sterilizing agent. Sandalwood's best sterilization: NaOCl at 10% & 15%.
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Moisan, Michel, Jean Barbeau, Marie-Charlotte Crevier, Jacques Pelletier, Nicolas Philip, and Bachir Saoudi. "Plasma sterilization. Methods and mechanisms." Pure and Applied Chemistry 74, no. 3 (2002): 349–58. http://dx.doi.org/10.1351/pac200274030349.

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Utilizing a plasma to achieve sterilization is a possible alternative to conventional sterilization means as far as sterilization of heat-sensitive materials and innocuity of sterilizing agents are concerned. A major issue of plasma sterilization is the respective roles of ultraviolet (UV) photons and reactive species such as atomic and molecular radicals. At reduced gas pressure (£10 torr) and in mixtures containing oxygen, the UV photons dominate the inactivation process, with a significant contribution of oxygen atoms as an erosion agent. Actually, as erosion of the spore progresses, the nu
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Pan, Dongmei, Rui Li, Yakai Li, et al. "Effects of manual washing with three alkaline sterilizing agent solutions on egg quality during storage." Food Chemistry 396 (December 2022): 133733. http://dx.doi.org/10.1016/j.foodchem.2022.133733.

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26

Hassen, Nurul Izzah, Noor Afiza Badaluddin, Zakiah Mustapha, and Dhiya Dalila Zawawi. "Identification and Prevention of Microbial Contaminants in Musa paradisiaca Tissue Culture." Malaysian Applied Biology 51, no. 5 (2022): 129–43. http://dx.doi.org/10.55230/mabjournal.v51i5.2374.

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Banana (Musa spp.) is an economically vital fruit crop in Malaysia and therefore, micropropagation was applied to meet the market demand for disease-free seedlings. However, microbial contamination is a significant obstacle in micropropagation techniques. In this research, the microorganisms that were present in the banana culture were characterized and the efficacy of antimicrobial and antifungal agents to inhibit contaminants was evaluated. Three bacterial and two fungal isolates were isolated from the contaminated culture. Klebsiella pneumoniae, Klebsiella quasipneumoniae, and Klebsiella va
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27

Karakoyun, Zeynep Nisa, Ömer Faruk Karakoyun, Sümeyye Gül Yılmaz, and Yalcin Golcuk. "A case report on unintentional ingestion of formaldehyde solution." Ege Tıp Dergisi 63, no. 2 (2023): 313–15. http://dx.doi.org/10.19161/etd.1352530.

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Formaldehyde (FA) or formaldehyde solution, whose industrial name is formalin ad systematic name is methanal (CH2O), is an organic compound in the aldehyde structure. Industrially, FA is used for its preservative and sterilizing agent properties. Fish farms use FA solutions against bacterial diseases in juvenile fish, protect to fish, and for disinfection purposes. Although FA is a frequently used substance in the industrial, laboratory, and cosmetic fields, the literature is very scare in terms of toxic doses or symptoms in cases of oral intakes. This case describes the clinical course and wr
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28

Belov, Sergey V., Yury K. Danileiko, Alexey B. Egorov, et al. "Sterilizer of Knives in the Meat Industry, Working by Activating Aqueous Solutions with Glow Discharge Plasma." Processes 10, no. 8 (2022): 1536. http://dx.doi.org/10.3390/pr10081536.

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The development of approaches for the non-thermal sterilization of instruments is an urgent task to ensure the safety of meat industry products, where the use of hot water leads to the formation of condensates and a deterioration in the hygienic condition of the premises. In this study, an installation for sterilizing knives was created, which works by activating aqueous salt solutions with a glow discharge. The power consumption of the installation reactor is only 125–150 Wh. The temperature rise of the sterilizing agent used is about 1.1 ± 0.2 °C/min/L. The effectiveness of the installation
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Takahashi, H. A., M. Minami, T. Aramaki, et al. "A Suitable procedure for preparing of water Samples used in Radiocarbon Intercomparison." Radiocarbon 61, no. 6 (2019): 1879–87. http://dx.doi.org/10.1017/rdc.2019.104.

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ABSTRACTWe studied a suitable procedure for preparing of water samples used in radiocarbon intercomparisons involving dissolved inorganic carbon (DIC). The water samples must have inter-batch consistency and stable 14C concentrations and no sterilizing agent (e.g., HgCl2) should be added, in order to avoid the production of hazardous material. Six water samples, containing widely different amounts and types of salts, DIC, and 14C concentrations (1–100 pMC), were prepared in order to assess the procedure. Sample consistency was investigated through δ13C and chemical compositions; their low vari
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Park, Hyunjun, Kyunghwan Chai, Eugene Park, et al. "Optimization of Paper-Based Alveolar-Mimicking SERS Sensor for High-Sensitivity Detection of Antifungal Agent." Biosensors 14, no. 12 (2024): 566. http://dx.doi.org/10.3390/bios14120566.

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Crystal violet (CV) is a disinfectant and antifungal agent used in aquaculture that plays a vital role in treating aquatic diseases and sterilizing water. However, its potential for strong toxicity, including carcinogenicity and mutagenicity, upon accumulation in the body raises concerns regarding its safe use. Therefore, there is a growing need for the quantitative detection of CV in its early application stages to ensure human safety. Recently, Raman spectroscopy-based surface-enhanced Raman scattering (SERS) detection research has been actively conducted; consequently, an alveolar-mimicking
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ANDRADE JÚNIOR, Francisco Patricio de, Thalyta Karem Ferreira ROMANO, Brenda Tamires de Medeiros LIMA, et al. "ETHYLENE OXIDE AS A STERILIZING AGENT: GENERAL CHARACTERISTICS, TOXICITY AND COMPARISON WITH OTHERS SANEANTES AND STERILIZATION METHODS." Periódico Tchê Química 16, no. 31 (2019): 175–85. http://dx.doi.org/10.52571/ptq.v16.n31.2019.181_periodico31_pgs_175_185.pdf.

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Ethylene oxide gas is highly toxic, highly flammable and explosive. It is also carcinogenic, mutagenic, teratogenic and neurotoxic. However, it is an excellent sterilant especially when applied to thermally sensitive medical and hospital materials. The present study aimed to carry out a literature review on ethylene oxide emphasizing its general characteristics, toxicity and comparison with other methods of sterilization. This is a narrative review, in which the retrieval of studies occurred in the databases Lilacs, Scielo, PubMed, Science Direct, Periódicos Capes and Theses Banks and disserta
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Gerulova, Kristina, Ondrej Tatarka, Tomas Stefko, Zuzana Szabova, and Jozef Fiala. "Effect of Ozone Application to Microbial Contaminated Samples of In-Use Metalworking Fluids." Advanced Materials Research 884-885 (January 2014): 277–82. http://dx.doi.org/10.4028/www.scientific.net/amr.884-885.277.

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Metalworking fluids are prone to bacterial contamination during the operational time. They are unwanted due their impact on the quality of the fluid, health impact of the microbial toxins, pathogens and degradation products of fluid. Typically bacteria are eliminating by the adding of biocides, which could be also harmless to operators health. Ozone is used as a sterilizing agent in many industrial applications, mostly in water disinfection. Wide varieties of microorganisms can be treated by the ozone. Ozone, a powerful disinfectant, is very efficient to inactivate resistant microorganisms und
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Khromova, T. M., L. V. Tashmatova, and O. V. Matsneva. "Application of various growth regulators in clonal micropropagation of black currant (Ribes Nigrum L.)." Вестник российской сельскохозяйственной науки, no. 1 (May 20, 2024): 30–34. http://dx.doi.org/10.31857/s2500208224010071.

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Currently, the optimization and development of new techniques for microclonal reproduction of black currant (Ribes nigrum L.) are one of the traditional objects of research of domestic and foreign scientists. It is noted that the success of reproduction depends on a number of factors: the timing of introduction into culture in vitro, the type of explants, the sterilizing agent, the composition of the nutrient medium. At the same time, the survival and growth of explants at each stage of reproduction depends not only on the salt composition of the nutrient medium, but also on growth regulators,
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Gibbs, P. E. "Male genital defect (Dumpton Syndrome) in the dog-whelk Nucella lapillus (Neogastropoda): Mendelian inheritance inferred, based on laboratory breeding experiments." Journal of the Marine Biological Association of the United Kingdom 85, no. 1 (2005): 143–50. http://dx.doi.org/10.1017/s0025315405010969h.

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Laboratory breeding of the dog-whelk, Nucella lapillus, has established that the male-sterilizing Dumpton Syndrome (DS)—underdevelopment, or non-development (aphally), of the penis, incomplete formation (non-closure) of the vas deferens, resulting in a split prostate—can be readily observed in male F1 progeny. Cultivated under high ambient concentrations of the antifouling agent tributyltin (TBT), DS-carrying females can be recognized by their lesser degree of masculinization (imposex): sterilization is thereby avoided. When Dumpton females are crossed, under high ambient TBT, with individuals
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Mane, Abhishek, Siddhi Cheulkar, Romil Arora, Aarti Singh, and M. A. Gulbarga. "Design and Fabrication of Portable PPE Kit Sterilization." International Journal for Research in Applied Science and Engineering Technology 10, no. 4 (2022): 2870–79. http://dx.doi.org/10.22214/ijraset.2022.41881.

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Abstract: Medical demands during the COVID-19 pandemic have triggered a grave shortage of medical-grade personal protective equipment (PPE), especially, N95 respirators. N95 respirators are critical for the personal protection of medical providers and others when being exposed to individuals with infections caused by the SARS- CoV-2 coronavirus. To address the shortage of PPE Kit & N95 respirators, innovative methods are needed to decontaminate coronaviruses from N95 respirators & PPE Kit, allowing them to be safely reused by healthcare workers. For this research, we use a commercial o
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Arya, Raj Kumar, George D. Verros, and Devyani Thapliyal. "Towards a Mathematical Model for the Viral Progression in the Pharynx." Healthcare 9, no. 12 (2021): 1766. http://dx.doi.org/10.3390/healthcare9121766.

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In this work, a comprehensive model for the viral progression in the pharynx has been developed. This one-dimension model considers both Fickian diffusion and convective flow coupled with chemical reactions, such as virus population growth, infected and uninfected cell accumulation as well as virus clearance. The effect of a sterilizing agent such as an alcoholic solution on the viral progression in the pharynx was taken into account and a parametric analysis for the effect of kinetic rate parameters on virus propagation was made. Moreover, different conditions caused by further medical treatm
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Mok, Chulkyoon, and Nam-Hoon Lee. "Ultraviolet Inactivation of Escherichia coli in Stainless Steel Cups." Food Engineering Progress 13, no. 2 (2009): 122–29. http://dx.doi.org/10.13050/foodengprog.2009.13.2.122.

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Ultraviolet (UV) is widely used as a sterilizing agent in restaurants and catering facilities in Korea. Efficacy of UV sterilizers (UVS) was investigated against E. coli on the inner bottom of stainless steel cups. UV intensity on the bottom of cups varied widely with the locations of cups in UVS, showing higher values at center while lower values at outskirts. The deviations in UV intensity were remarkable on top shelf, but alleviated as proceeded to middle and bottom shelves. Inactivation of E. coli was proportional to the UV intensity and treatment time, consequently to UV dose, and showed
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Arsenijevic, Zorana, Zeljko Grbavcic, Bosko Grbic, Nenad Radic, Radmila Garic-Grulovic, and Mihal Djuris. "Removal of ethylene oxide from waste gases by absorption." Chemical Industry 65, no. 4 (2011): 389–95. http://dx.doi.org/10.2298/hemind110329026a.

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Ethylene oxide (EtO) is an organic compound, which is used as starting material in the production of polymers and as sterilizing agent for thermolabile materials. Although ethylene oxide is not common as an organic pollutant, its removal from numerous emission sources (e.g. ethylene oxide production plants or food and pharmaceutical sterilizing units) is of the crucial importance because of its mutagenic, teratogenic and cancerogenic effect on human health. The objective of this paper is the experimental investigation of ethylene oxide (EtO) absorption in diluted aqueous solution of sulfuric a
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Ali, Moazam, Misbah Ijaz, Asad Manzoor, et al. "Efficacy of Azadirachta indica (Neem) leaf extract and hypertonic saline solution as intratesticular chemical sterilizing agents in dogs." Brazilian Journal of Veterinary Research and Animal Science 57, no. 4 (2020): e171582. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2020.171582.

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Castration refers to induced sterility via physical, chemical, or hormonal methods. Chemical castration is an efficient and reliable technique in contrast to other sterilization procedures as it is less painful to physical methods and cost‑effective to hormonal methods. Azadirachta indica (neem), is a charismatic plant as its leaves possess anti-inflammatory, anti‑microbial, and anti-androgenic chattels. To abate the escalating human population in South Asia, neem oil and neem leaf extract have been effectively used as a contraceptive agent. The key determinant of the current study was to eval
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Eremina, Olga Yurevna, and Veronika Valentinovna Olifer. "Boric acid-based baits against pharaoh ants." Disinfection affairs, no. 1 (March 2021): 41–47. http://dx.doi.org/10.35411/2076-457x-2021-1-41-47.

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For control of the Pharaoh ant, liquid baits based on 5–6 % boric acid or sodium tetraborate, with a high content of food attractant and an agent that prevents drying, are effective. The death of workers, queens and brood occurs within 2–4 weeks. In laboratory conditions, when colonies of the Pharaoh ant feed with such baits, depletion of the colony was noted by the 4th week of the experiment (death of queen was 75–100 %, and critical decrease in the number of live workers and brood was occured). Dry boric baits do not significantly affect the viability of ant colonies. Solutions of sodium tet
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Mravlje, Jure, Marjana Regvar, and Katarina Vogel-Mikuš. "Development of Cold Plasma Technologies for Surface Decontamination of Seed Fungal Pathogens: Present Status and Perspectives." Journal of Fungi 7, no. 8 (2021): 650. http://dx.doi.org/10.3390/jof7080650.

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In view of the ever-growing human population and global environmental crisis, new technologies are emerging in all fields of our life. In the last two decades, the development of cold plasma (CP) technology has offered a promising and environmentally friendly solution for addressing global food security problems. Besides many positive effects, such as promoting seed germination, plant growth, and development, CP can also serve as a surface sterilizing agent. It can be considered a method for decontamination of microorganisms on the seed surface alternative to the traditional use of fungicides.
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Gaudet, Ryan G., Shiwei Zhu, Anushka Halder, et al. "A human apolipoprotein L with detergent-like activity kills intracellular pathogens." Science 373, no. 6552 (2021): eabf8113. http://dx.doi.org/10.1126/science.abf8113.

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Activation of cell-autonomous defense by the immune cytokine interferon-γ (IFN-γ) is critical to the control of life-threatening infections in humans. IFN-γ induces the expression of hundreds of host proteins in all nucleated cells and tissues, yet many of these proteins remain uncharacterized. We screened 19,050 human genes by CRISPR-Cas9 mutagenesis and identified IFN-γ–induced apolipoprotein L3 (APOL3) as a potent bactericidal agent protecting multiple non–immune barrier cell types against infection. Canonical apolipoproteins typically solubilize mammalian lipids for extracellular transport
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Whitlock, Greg C., Caroline Rowland, Roman A. Lukaszewski, et al. "Role of effector B and T lymphocytes and cytokine production in the protective host response to vaccination with heat killed bacilli against Burkholderia mallei (43.14)." Journal of Immunology 178, no. 1_Supplement (2007): S39. http://dx.doi.org/10.4049/jimmunol.178.supp.43.14.

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Abstract Burkholderia mallei, the etiologic agent of glanders disease, is a gram-negative, intracellular bacterium for which no vaccine is available. Consequently, we are interested in defining protective host immune responses to B. mallei infection with the goal of developing candidate vaccines. Here, we report on initial cell/cytokine depletion studies to investigate the possible role these effectors may play in response to vaccination with heat killed bacilli in a susceptible BALB/c mouse model of infection. While protection with heat killed bacilli does not result in sterilizing immunity,
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Richard, Khumoekae, Cole Schonhofer, Leila B. Giron, et al. "The African natural product knipholone anthrone and its analogue anthralin (dithranol) enhance HIV-1 latency reversal." Journal of Biological Chemistry 295, no. 41 (2020): 14084–99. http://dx.doi.org/10.1074/jbc.ra120.013031.

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A sterilizing or functional cure for HIV is currently precluded by resting CD4+ T cells that harbor latent but replication-competent provirus. The “shock-and-kill” pharmacological ap-proach aims to reactivate provirus expression in the presence of antiretroviral therapy and target virus-expressing cells for elimination. However, no latency reversal agent (LRA) to date effectively clears viral reservoirs in humans, suggesting a need for new LRAs and LRA combinations. Here, we screened 216 compounds from the pan-African Natural Product Library and identified knipholone anthrone (KA) and its basi
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Naitou, Shigezo, and Hirofumi Takahara. "Recent Developments in Food and Agricultural uses of Ozone as an Antimicrobial Agent-Food Packaging Film Sterilizing Machine using Ozone." Ozone: Science & Engineering 30, no. 1 (2008): 81–87. http://dx.doi.org/10.1080/01919510701813376.

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Talaviya, Harshang, Hiteshvari Korat, KAILASHPATI TRIPATHI, Muzaffar Hasan, and M. K. Mahatma. "Analytical Methods and Regulatory Perspectives on Residual Ethylene Oxide and Its Derivatives in Food Products." International Journal of Seed Spices 14, no. 1 (2025): 1–15. https://doi.org/10.56093/ijss.v14i1.1.

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Ethylene oxide (EtO), a crucial industrial chemical, has diverse applications ranging from the production of ethylene glycol to its use as a sterilizing agent for medical equipment and a fumigant for food and textiles. However, its potential health risks, particularly carcinogenicity, have raised significant concerns. This review explores the synthesis, chemical properties, and metabolism of EtO, focusing on its transformation into 2-chloroethanol (2-CE). Here we described the industrial uses of EtO and the implications of its residues in food products, particularly spices, which have recently
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Buchenkov, I. E., V. O. Lemiasheuski, A. G. Sysa, Sui Xin, Yang Liu, and Song Zengyi. "Optimization of conditions for microclonal propagation of SALVIA PRATENSIS L." BIO Web of Conferences 179 (2025): 13004. https://doi.org/10.1051/bioconf/202517913004.

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The possibility of introducing Salvia pratensis, a protected medicinal plant of the Republic of Belarus, into in vitro culture for mass reproduction and return to natural ecosystems, as well as practical use as medicinal plant raw materials, has been studied. A sterilization regime for Salvia pratensis when introducing it into culture has been identified, which provides the best ratio of the number of sterile plant explants and the number of viable seeds. The optimal sterilizing agent is 0.1% silver nitrate solution with an exposure time of 20 minutes. The effect of various concentrations of g
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ROTANOV, S. U., N. V. FRIGO, and A. A. KUBANOV. "Ep syphilis expert panel of control materials for serological diagnostics of syphilis." Vestnik dermatologii i venerologii 87, no. 2 (2011): 32–37. http://dx.doi.org/10.25208/vdv986.

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GNTSD Rosmedtekhnologiy (State Research Center for Dermatology and Venereology of the Federal Agency for High-Technology Medical Aid) created a set of agents entitled "EP SYPHILIS expert panel of reference serums for serological diagnostics of syphilis" intended for internal laboratory and external quality control for these types of clinical laboratory tests. The expert panel includes four samples of reference materials: two samples containing the anti-T. pallidum antibody in high and low concentrations, and two other samples without the antibodies. According to the international recommendatio
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Berestneva, Yuliya, and Alina Mezhevova. "Selection of the optimal sterilization protocol of safflower seeds by the “Lysoformin-3000”." E3S Web of Conferences 431 (2023): 01024. http://dx.doi.org/10.1051/e3sconf/202343101024.

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Safflower (Carthamus tinctorius L.) is an economically important oil seed crop. Contamination of its seed leads to a decrease in seed germination, crop yields and product quality. The research of determination of the safflower seeds optimal sterilization protocol by the "Lysoformin-3000" at the stage of introduction to the in vitro culture was carried out. The research consisted of several experiments. The first stage of the study included the seeds treatment by a sterilizing agent with a concentration of 0.5 % to 5 % and an exposure time of 3 and 7 minutes, followed by germination in Petri di
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Sharma, Viney, Pawan Mishra, Suman Sharma, and Narinder Singh. "Importance of ETO Sterilization in the Modern Era-A Review." International Research Journal of Ayurveda & Yoga 06, no. 07 (2023): 149–52. http://dx.doi.org/10.47223/irjay.2023.6722.

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Introduction-Model sterilizations of genuine medical device items with a known composition showed to be more effective than evaluation in medical devices from medical institutions for research of the behavior of various polymers in the sterilization process. Aim and Objective-To study the importance of eto-Sterilization in the Modern Era. Material and Methods-Various modern text books, Journals and websites were referred for this study. Discussion and Conclusion-Ethylene oxide gas is typically used to sterilize disposable medical equipment. When this sterilization technique is utilized, the me
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